Digital Commons Network^™

The Effects Of Nuclear Factor Erythroid 2 (Nfe2)-Related Factor 2 (Nrf2) Activation In Preclinical Models Of Peripheral Neuropathic Pain, Paramita Basu, Dayna L. Averitt, Camelia Maier, Arpita Basu

Kinesiology and Nutrition Sciences Faculty Research

Oxidative stress, resulting from an imbalance between the formation of damaging free radicals and availability of protective antioxidants, can contribute to peripheral neuropathic pain conditions. Reactive oxygen and nitrogen species, as well as products of the mitochondrial metabolism such as superoxide anions, hydrogen peroxide, and hydroxyl radicals, are common free radicals. Nuclear factor erythroid 2 (NFE2)-related factor 2 (Nrf2) is a transcription factor encoded by the NFE2L2 gene and is a member of the cap ‘n’ collar subfamily of basic region leucine zipper transcription factors. Under normal physiological conditions, Nrf2 remains bound to Kelch-like ECH-associated protein 1 in the cytoplasm …

Recent Advances In Probiotic Application In Animal Health And Nutrition: A Review, Sarayu Bhogoju, Samuel Nahashon

Internal Medicine Faculty Publications

Biotechnological advances in animal health and nutrition continue to play a significant role in the improvement of animal health, growth, and production performance. These biotechnological advancements, especially the use of direct-fed microbials, also termed probiotics, those genetically modified and otherwise, have minimized many challenges facing livestock production around the world. Such advancements result in healthy animals and animal products, such as meat, for a growing population worldwide. Increasing demand for productivity, healthy animals, and consumer food safety concerns, especially those emanating from excessive use of antibiotics or growth promoters, are a driving force for investing in safer alternatives, such as …

Effects Of Three Different Modes Of Resistance Training On Appetite Hormones In Males With Obesity, Ali Ataeinosrat, Marjan Mosalman Haghighi, Hossein Abednatanzi, Mohammad Soltani, Abbass Ghanbari-Niaki, Akbar Nouri-Habashi, Sadegh Amani-Shalamzari, Ali Mossayebi, Mitra Khademosharie, Kelly E. Johnson, Trisha A. Vandusseldorp, Ayoub Saeidi, Hassane Zouhal

Kinesiology

Purpose: This study explored the effect of three different modes of resistance training on appetite hormones [leptin, ghrelin, cholecystokinin (CCK), glucagon-like peptide-1 (GLP-1), and peptide tyrosine–tyrosine (PYY)], cardiometabolic and anthropometric measures in males with obesity. Methods: Forty-four males with obesity (age: 27.5 ± 9.4 yrs.; mean weight: 93.2 ± 2.2 kg, body mass index: 32.9 ± 1.2 kg/m²) were randomized to traditional resistance training (TRT, n = 11), circuit resistance training (CRT, n = 11), interval resistance training (IRT, n = 11) or control (C, n = 11) groups. All resistance training groups received 50 min of supervised …

Imaging Techniques For Aortic Aneurysms And Dissections In Mice: Comparisons Of Ex Vivo, In Situ, And Ultrasound Approaches, Sohei Ito, Hong S. Lu, Alan Daugherty, Hisashi Sawada

Saha Cardiovascular Research Center Faculty Publications

Aortic aneurysms and dissections are life-threatening conditions that have a high risk for lethal bleeding and organ malperfusion. Many studies have investigated the molecular basis of these diseases using mouse models. In mice, ex vivo, in situ, and ultrasound imaging are major approaches to evaluate aortic diameters, a common parameter to determine the severity of aortic aneurysms. However, accurate evaluations of aortic dimensions by these imaging approaches could be challenging due to pathological features of aortic aneurysms. Currently, there is no standardized mode to assess aortic dissections in mice. It is important to understand the characteristics of each approach for …

Changes In Apolipoprotein Abundance Dominate Proteome Responses To Prolonged Fasting In Elephant Seals, Jane I. Khudyakov, Rachel R. Holser, Craig A. Vierra, Serena T. Ly, Theron K. Niel, Basma M. Hasan, Daniel E. Crocker, Daniel P. Costa

Pacific Faculty Work

Unlike many animals that reduce activity during fasting, northern elephant seals (NES) undergo prolonged fasting during energyintensive life-history stages such as reproduction and molting, fueling fasting energy needs by mobilizing fat stores accrued during foraging. NES display several unique metabolic features such as high fasting metabolic rates, elevated blood lipid and high-density lipoprotein (HDL) cholesterol levels, efficient protein sparing and resistance to oxidative stress during fasting. However, the cellular mechanisms that regulate these adaptations are still not fully understood. To examine how metabolic coordination is achieved during prolonged fasting, we profiled changes in blubber, skeletal muscle and plasma proteomes of …

The Ability Of Resveratrol To Attenuate Ovalbumin-Mediated Allergic Asthma Is Associated With Changes In Microbiota Involving The Gut-Lung Axis, Enhanced Barrier Function And Decreased Inflammation In The Lungs, Prakash Nagarkatti, Esraah Alharris, Hasan Alghetaa, Juhua Zhou, Mitzi Nagarkatti

Faculty Publications

Asthma is a chronic respiratory disease highly prevalent worldwide. Recent studies have suggested a role for microbiome-associated gut–lung axis in asthma development. In the current study, we investigated if Resveratrol (RES), a plant-based polyphenol, can attenuate ovalbumin (OVA)-induced murine allergic asthma, and if so, the role of microbiome in the gut–lung axis in this process. We found that RES attenuated allergic asthma with significant improvements in pulmonary functions in OVA-exposed mice when tested using plethysmography for frequency (F), mean volume (MV), specific airway resistance (sRaw), and delay time(dT). RES treatment also suppressed inflammatory cytokines in the lungs. RES modulated lung …

Pathological Human Tau Induces Alterations In The Brain Insulin Signaling Cascade, Abdeslem El Idrissi, Alejandra Del Carmen Alonso

Publications and Research

The process of neurodegeneration in Alzheimer’s disease has been associated with a disruption of insulin signaling cascade in neurons, and to insulin resistance. T2DM correlates with Alzheimer’s disease, but mechanisms of interaction are unknown. We have developed a mouse model of tau induced neurodegeneration expressing pseudo-phosphorylated tau [Pathological Human Tau (PH-Tau)] in neurons. This model (PH-Tau-Tg) recapitulated cognitive decline and neurodegeneration observed in AD. In this study we examined if expression of PH-Tau could affect neuronal excitability and insulin receptor signaling. Neuronal excitability was investigated using intracerebral recordings of extracellular field potentials from prefrontal cortex after insulin and kainic acid …

Activation Of Ras/Mapk Pathway Confers Mcl-1 Mediated Acquired Resistance To Bcl-2 Inhibitor Venetoclax In Acute Myeloid Leukemia, Qi Zhang, Bridget Riley-Gillis, Lina Han, Yannan Jia, Alessia Lodi, Haijiao Zhang, Saravanan Ganesan, Rongqing Pan, Sergej N Konoplev, Shannon R Sweeney, Jeremy A Ryan, Yulia Jitkova, Kenneth Dunner, Shaun E Grosskurth, Priyanka Vijay, Sujana Ghosh, Charles Lu, Wencai Ma, Stephen Kurtz, Vivian R Ruvolo, Helen Ma, Connie C Weng, Cassandra L Ramage, Natalia Baran, Ce Shi, Tianyu Cai, Richard Eric Davis, Venkata L Battula, Yingchang Mi, Jing Wang, Courtney D Dinardo, Michael Andreeff, Jeffery W Tyner, Aaron Schimmer, Anthony Letai, Rose Ann Padua, Carlos E Bueso-Ramos, Stefano Tiziani, Joel Leverson, Relja Popovic, Marina Konopleva

Faculty, Staff and Student Publications

Despite high initial response rates, acute myeloid leukemia (AML) treated with the BCL-2-selective inhibitor venetoclax (VEN) alone or in combinations commonly acquires resistance. We performed gene/protein expression, metabolomic and methylation analyses of isogenic AML cell lines sensitive or resistant to VEN, and identified the activation of RAS/MAPK pathway, leading to increased stability and higher levels of MCL-1 protein, as a major acquired mechanism of VEN resistance. MCL-1 sustained survival and maintained mitochondrial respiration in VEN-RE cells, which had impaired electron transport chain (ETC) complex II activity, and MCL-1 silencing or pharmacologic inhibition restored VEN sensitivity. In support of the importance …

Natural Biomaterial Honey-Based Resistive Switching Device For Artificial Synapse In Neuromorphic Systems, Brandon Sueoka, Kuan Yew Cheong, Feng Zhao

Electrical and Computer Engineering Faculty Research & Creative Works

Resistive switching is a promising technology for artificial synapses, the most critical component and building block of a neural network for brain-inspired neuromorphic computing. The artificial synapse is capable of emulating a signal process and memory functions of biological synapses. The artificial synapse fabricated by natural bioorganic materials is essential for developing soft, flexible, and biocompatible electronics and sustainable, biodegradable, and environmentally friendly neuromorphic systems. In this work, a natural biomaterial - honey based resistive switching device - was demonstrated to emulate some important functionalities of biological synapses, including synaptic potentiation and depression, short-term and long-term memory, spatial summation, and …

Dose-Intensified Stereotactic Ablative Radiation For Localized Prostate Cancer, Lily Chen, Bhavani S. Gannavarapu, University Of Texas Southwestern Medical Center At Dallas, Michael R. Folkert, Michael Dohopolski, Ang Gao, Chul Ahn, Jeffrey Cadeddu, Aditya Bagrodia

School of Medicine Publications

Purpose: Stereotactic ablative radiation (SAbR) has been increasingly used in prostate cancer (PCa) given its convenience and cost efficacy. Optimal doses remain poorly defined with limited prospective comparative trials and long-term safety/efficacy data at higher dose levels. We analyzed toxicity and outcomes for SAbR in men with localized PCa at escalated 45 Gy in 5 fractions.

Methods and Materials: This study retrospectively analyzed men from 2015 to 2019 with PCa who received linear-accelerator-based SAbR to 45 Gy in 5 fractions, along with perirectal hydrogel spacer, fiducial placement, and MRI-based planning. Disease control outcomes were calculated from end of treatment. Minimally …

Synthesis, Characterization, Photocatalysis, And Antibacterial Study Of Wo3, Mxene And Wo3/Mxene Nanocomposite, Al-Zoha Warsi, Fatima Aziz, Sonia Zulfiqar, Sajjad Haider

Faculty Journal Articles

Tungsten oxide (WO3), MXene, and an WO3/MXene nanocomposite were synthesized to study their photocatalytic and biological applications. Tungsten oxide was synthesized by an easy and cost-effective hydrothermal method, and its composite with MXene was prepared through the sonication method. The synthesized tungsten oxide, MXene, and its composite were characterized by X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), Fourier transform infrared (FTIR), energy-dispersive X-ray analysis (EDX), and Brunauer–Emmett–Teller (BET) for their structural, morphological, spectral, elemental and surface area analysis, respectively. The crystallite size of WO3 calculated from XRD was ~10 nm, the particle size of WO3 was 130 nm, …

Synthesis, Characterization And Host-Guest Complexation Of Asplatin: Improved In Vitro Cytotoxicity And Biocompatibility As Compared To Cisplatin, Sherif Ashraf Fahmy, Fortuna Ponte, Giulia Grande, Iten M. Fawzy

Faculty Journal Articles

Para-sulfocalix[n]arenes are promising host molecules that can accommodate various chemotherapeutic drugs. Pt(IV)-based complexes, including satraplatin and asplatin, are promising alternatives that overcome the shortcomings of Pt(II) complexes. In this study, asplatin has been synthesized by fusing acetylsalicylic acid (aspirin) and cisplatin. Furthermore, it has been characterized using 1H NMR, mass spectrometry, elemental analysis, and UHPLC. A host-guest complex of asplatin and p-sulfocalix[4]arene (PSC4) has been developed and characterized using UV, Job’s plot analysis, HPLC, and density functional theory (DFT) calculations. The experimental and computational investigations propose that a 1:1 complex between asplatin and PSC4 is formed. The stability constant of …

Covid-19 And Myocarditis: A Brief Review, Fatma Taher, Athar Eysa, Dalia Fahmy, Ahmed Shalaby, Ali Mahmoud, Moumen El-Melegy, Ahmed Abdel Khalek Abdel Razek, Ayman El-Baz

All Works

Cardiovascular complications (especially myocarditis) related to COVID-19 viral infection are not well understood, nor do they possess a well recognized diagnostic protocol as most of our information regarding this issue was derived from case reports. In this article we extract data from all published case reports in the second half of 2020 to summarize the theories of pathogenesis and explore the value of each diagnostic test including clinical, lab, ECG, ECHO, cardiac MRI and endomyocardial biopsy. These tests provide information that explain the mechanism of development of myocarditis that further paves the way for better management.

Eml1 Is Essential For Retinal Photoreceptor Migration And Survival, Deepak Poria, Chi Sun, Andrea Santeford, Michel Kielar, Rajendra S Apte, Oleg G Kisselev, Shimming Chen, Vladimir J Kefalov

Open Access Publications

Calcium regulates the response sensitivity, kinetics and adaptation in photoreceptors. In striped bass cones, this calcium feedback includes direct modulation of the transduction cyclic nucleotide-gated (CNG) channels by the calcium-binding protein CNG-modulin. However, the possible role of EML1, the mammalian homolog of CNG-modulin, in modulating phototransduction in mammalian photoreceptors has not been examined. Here, we used mice expressing mutant Eml1 to investigate its role in the development and function of mouse photoreceptors using immunostaining, in-vivo and ex-vivo retinal recordings, and single-cell suction recordings. We found that the mutation of Eml1 causes significant changes in the mouse retinal structure characterized by …

Understanding Information Needs And Barriers To Accessing Health Information Across All Stages Of Pregnancy: Systematic Review, Yu Lu, Laura A Barrett, Rebecca Z Lin, Muhammad Amith, Cui Tao, Zhe He

Open Access Publications

BACKGROUND: Understanding consumers' health information needs across all stages of the pregnancy trajectory is crucial to the development of mechanisms that allow them to retrieve high-quality, customized, and layperson-friendly health information.

OBJECTIVE: The objective of this study was to identify research gaps in pregnancy-related consumer information needs and available information from different sources.

METHODS: We conducted a systematic review of CINAHL, Cochrane, PubMed, and Web of Science for relevant articles that were published from 2009 to 2019. The quality of the included articles was assessed using the Critical Appraisal Skills Program. A descriptive data analysis was performed on these articles. …

Everyday Creativity: Singing Goddesses In The Himalayan Foothills, Nadia Chana

Yale Journal of Music & Religion

A book review is presented for Kirin Narayan, Everyday Creativity: Singing Goddesses in the Himalayan Foothills (Chicago: University of Chicago Press, 2016).

A Scalable And Participatory Sustainable Rangeland Management Toolkit With A Holistic And Multidisciplinary Approach To Rehabilitate Degraded Rangelands, Mounir Louhaichi, Mouldi Gamoun, Sawsan Hassan

IGC Proceedings (1977-2023)

Rangelands contribute significantly toward improving livelihoods, offering food security, trade and tourism for pastoral communities. Numerous challenges include poor government policies, loss of indigenous knowledge and top-down approaches toward sustainable rangeland rehabilitation that often fail to consider local development adoption and sustainability. In such situations, effective management is needed for sustainable rangeland ecosystem goods and services in a context characterized by rainfall unreliability, poor soil nutrient status and high uncontrolled grazing. This paper presents a new comprehensive toolkit for identifying and combining suitable and site-specific interventions aimed at reversing the trend of degraded arid rangelands. This toolbox is founded on …

Pastoralist Use Of Reseeded Fields For Additional Resilience To Climate Variability Through Alternate Livelihood Activities, D. W. Githu, J. S. Fehmi, M. Mcclaran, M. Elizabeth, A. Josephson

IGC Proceedings (1977-2023)

Land degradation, climate variability, socio-economic changes and population increase are some of the factors that shrink grazing lands making forage availability less predictable and affecting the resilience of pastoralists communities. Reseeding as an intervention can rehabilitate degraded lands for profitable use through offering pastoralists alternative sources of livelihood and encourage livelihood diversification. This study evaluated two pastoral communities, the Tugen and the Njemps from Baringo County, Kenya, who have owned, managed and used reseeded fields to study the dynamics of reseeding land. A total of 193 pastoralist households were interviewed. These two pastoral communities used their fields for: dry season …

Utilizing Perennial Grass Species’ Population Patterns To Detect Looming Desertification Tipping Points In Semi-Arid Regions, F. Munyebvu-Chambara, W. C. Nesongano, M. C. Bilton, A. Linstädter

IGC Proceedings (1977-2023)

Namibia is one of the global dryland regions facing desertification threats due to overgrazing and recurrent drought. This has disastrous effects on forage provision and consequently on local livelihoods. Unfortunately, the mechanisms underlying sudden shifts of rangeland ecosystems towards a desertified state are still poorly understood. The ability to predict desertification tipping points with the aid of suitable ecological indicators is critical to ensure sustainable management of rangeland resources, which are a backbone of rural livelihood in drylands worldwide. Accordingly, we aimed to identify key perennial grass species that can be used as early-warning indicators for desertification tipping points; and …

Effects Of Hypoxia And Reperfusion On The Neuronal Plasma Membrane Calcium Pump, Kailey Kowalski, Ehab Sarsour, Asma Zaidi

Faculty and Staff Publications

Stroke is the fifth cause of death and a leading cause of disability in the United States. In an ischemic stroke, blood flow to the brain is blocked, leading to lack of oxygen, ATP, membrane damage and eventual neuronal cell death. Therapies to treat ischemic stroke restore blood flow but cause further neuronal damage by generating reactive oxygen species (ROS) and creating oxidative stress. The plasma membrane calcium ATPase (PMCA) is a high-affinity calcium transporter critical for the maintenance of calcium homeostasis and optimal neuronal function. Previous studies from our laboratory have shown a unique sensitivity of PMCA to ROS. …

How Much Margin Is Left For Degrading Agricultural Soils? The Coming Soil Crises, Maheteme Gebremedhin, Mark S. Coyne, Karamat R. Sistani

Plant and Soil Sciences Faculty Publications

Agricultural soils are in peril. Multiple lines of observational and empirical evidence suggest that we are losing the world’s fertile soils at an alarming rate, worsening the on-going global food crisis. It is increasingly clear that the risk of soil crises driven by erratic precipitation, warming air, and farming mismanagement is coming sooner rather than later. At this critical time, society cannot avoid looking for ways to curb soil crises. We argue that now is the right time for science-based mitigation strategies and new insights to protect soils. We offer four research priority areas that society needs to address. Arresting …

Spatial And Temporal Variation Of Selected Water Quality Parameters In The Tanzanian Side Of Lake Victoria, Richard Joseph Kimwaga

Tanzania Journal of Engineering and Technology (TJET)

Lake Victoria’s water quality is increasingly becoming under heavy pressure mainly due to land based activities and aerial inputs which are taking place within the basin. This study was carried out to assess the spatial, including vertical and temporal, variation of the water quality of Lake Victoria on the Tanzanian side. Historical water quality data for the period from 2000 to 2016 was collected. Temperature, pH, Dissolved Oxygen (DO) and Turbidity were selected for study since they are the mostly measured and monitored water quality parameters. Land use-land cover changes were analysed using ArcGIS. ILWIS 3.7 software was used to …

Single-Cell Transcriptome And Network Analyses Unveil Key Transcription Factors Regulating Mesophyll Cell Development In Maize, Shentong Tao, Peng Liu, Yining Shi, Yilong Feng, Jingjing Gao, Lifen Chen, Hairong Wei, Et, Al,

Michigan Tech Publications, Part 1

BACKGROUND: Maize mesophyll (M) cells play important roles in various biological processes such as photosynthesis II and secondary metabolism. Functional differentiation occurs during M-cell development, but the underlying mechanisms for regulating M-cell development are largely unknown. RESULTS: We conducted single-cell RNA sequencing (scRNA-seq) to profile transcripts in maize leaves. We then identified coregulated modules by analyzing the resulting pseudo-time-series data through gene regulatory network analyses. , , , and () families were highly expressed in the early stage, whereas () and families were highly expressed in the late stage of M-cell development. Construction of regulatory networks revealed that these transcript …

Overview Of Cutting-Edge Innovation In Life Sciences: Learning From Perspective Of Broad Institute, Runzhou Zhao, Shu Liu

Bulletin of Chinese Academy of Sciences (Chinese Version)

Since its establishment in 2004, the Broad Institute in the United States has been dedicated to combination research innovation with management innovation. In less than 10 years, it has been enlisted as one of the world-class life sciences research institutions and become the leader and signpost of cutting-edge innovation in life sciences. Its unique development model is worthy of reference. This study showed that the high-quality innovation and development of the Broad Institute is inseparable from:(1) its focus on the diagnosis and treatment needs of major diseases for scientific research; (2) the opening up of the channel from basic research …

Scientific And Technological Reasons, Contents And Corresponding Policies Of Constructing “Coastal Grass Belt”, Weihua Xu, Jianlin Wang, Xiaojing Liu, Qi Xie, Weicai Yang, Xiaofeng Cao, Zhensheng Li

Bulletin of Chinese Academy of Sciences (Chinese Version)

Not only the grain rations must be secured absolutely in China, but also the feed grains. However, the limited arable land resource in China determines that grass planting shall not "compete for land with staple food". Planting high quality salt-tolerant forage grass on the saline wasteland and developing animal husbandry production can not only meet the demand of increasing proportion of animal protein in Chinese diet structure, but more importantly, it can help to solve the problems of insufficient forage planting and import over-dependence. Hence it plays an important role in guaranteeing national food security. Therefore, it is necessary to …

Prediction Of Soil Water Content And Electrical Conductivity Using Random Forest Methods With Uav Multispectral And Ground-Coupled Geophysical Data, Yunyi Guan, Katherine R. Grote, Joel Schott, Kelsi Leverett

Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works

The volumetric water content (VWC) of soil is a critical parameter in agriculture, as VWC strongly influences crop yield, provides nutrients to plants, and maintains the microbes that are needed for the biological health of the soil. Measuring VWC is difficult, as it is spatially and tempo-rally heterogeneous, and most agricultural producers use point measurements that cannot fully capture this parameter. Electrical conductivity (EC) is another soil parameter that is useful in agricul-ture, since it can be used to indicate soil salinity, soil texture, and plant nutrient availability. Soil EC is also very heterogeneous; measuring EC using conventional soil sampling …

Decreases In G0-G1 Switch 2 Expression And Increases In Lipid Peroxidation Impaired Wound Healing In Aging Normal Human Fibroblasts, Kami Pearson, Spoorthi Hiremagalore, Jenna Fussell, Amanda Kalen, Prabhat Goswami, Ehab Sarsour

Faculty and Staff Publications

Human longevity is increasing; in fact, the United States Census Bureau predicts that there will be a 10% increase in individuals 65 y of age and older by 2050. Unfortunately, increases in longevity is also associated with increased risk factors for numerous health issues, and decreased effectiveness of medical interventions. Aging cells lose their ability to renew and repair damage. RNA sequencing and bioinformatics analysis of primary cultures of Normal Human Fibroblasts (NHFs) from healthy donors of different ages (3 d – 70 y) ranked lipid metabolism as the number one metabolic process that is significantly altered during aging. Using …

Rescuing Calcium Homeostasis With A Little Oil, Ronithe Senatus, Asma Zaidi, Ehab Sarsour

Faculty and Staff Publications

The plasma membrane calcium ATPase (PMCA) is a neuronal calcium transporter that plays a critical role in regulating intracellular calcium levels by pumping calcium out of cells, against its 10,000-fold concentration gradient. Previous studies from our laboratory have shown that PMCA undergoes a progressive reduction in activity and protein levels with increasing age and in neurodegenerative disorders. We have further demonstrated the unique sensitivity of PMCA to oxidative stress. Loss of PMCA causes dysregulation of calcium homeostasis, creates calcium toxicity, and induces cell death. A mechanism to prevent loss of PMCA and/or boost its activity would serve as a therapeutic …

Type Ii Diabetes – A Disease Of Unregulated Calcium?, Emily Hagan, Ehab Sarsour, Asma Zaidi

Faculty and Staff Publications

The plasma membrane calcium ATPase (PMCA) is a high affinity ion transporter that pumps Ca2+ out of cells to maintain optimal intracellular Ca2+ levels essential for cell function. Previous studies from our laboratory have shown the PMCA to be uniquely sensitive to oxidative stress. Exposure to reactive oxygen species (ROS) of physiological relevance caused dramatic inactivation, aggregation, and proteolytic degradation of the protein. Type 2 diabetes, an extremely prevalent metabolic pathophysiological condition is associated with oxidative stress. Cellular damage caused by ROS exacerbates diabetes and paves the way for the development of other pathological conditions such as cardiovascular disease and …

An Inverse Correlation Between Hypoxia Inducible Factor-1Α Expression And Lipid Droplets Associated With A Decline In The Proliferative Index Of Aging Normal Human Fibroblasts, Spoorthi Hiremagalore, Kami Pearson, Jyung Son, Amanda Kalen, Prabhat Goswami, Ehab Sarsour

Faculty and Staff Publications

Aging is a critical risk factor for numerous chronic diseases, and it presents a rising clinical problem as approximately 19 percent of the US population is predicted to be 65 y and older by 2030 (U.S. Census Bureau). However, the mechanisms that regulate the process of aging are still not well understood. Results from RNA sequencing and bioinformatics analysis showed a significant difference in global gene expression between old (58 - 70 y) compared to young (3 d – 12 y) Normal Human Fibroblasts (NHFs). Gene set enrichment analysis ranked lipid metabolism as the top pathway that is altered in …