Digital Commons Network^™

Fungal Associations In An Urban Forest, Jordan Leis

Senior Theses

Fungal communities worldwide are scarcely understood in part due to their vast networks stretching underground or woven into the biomass of plant life. Even macrofungi which produce fruiting bodies colloquially called ‘mushrooms’ hold a transient lifestyle above soil. Urbanization has introduced another challenge towards studying fungi as highly disturbed soils and a lack of preferred host plant species can alter fungal community composition and prevalence. In the summer of 2020 the macrofungal community of Forest Park in Portland, Oregon was surveyed in addition to control sites in the Mount Hood National Forest. Fungi samples were identified by genus and when …

Characterization Of Streptomyces Nymphaeiformis Sp. Nov., And Its Taxonomic Relatedness To Other Polyhydroxybutyrate-Degrading Streptomycetes, Gary R. Hix, Muhammad S. Khan, Mikayla T. Miller, Elisha C. Napier, Allison L. O'Brien, Roger P. White, Stephen F. Baron Ph.D.

Biology Faculty Scholarship

A polyhydroxybutyrate (PHB)-degrading actinomycete, strain SFB5A^T, was identified as a species of Streptomyces based on its membrane fatty acid profile and the presence of LL-diaminopimelic acid in the cell wall. It formed sporulating mycelia on most agar media, but flat or wrinkled, moist colonies on trypticase soy agar. Spores were smooth, cylindrical, and borne on long, straight to flexuous chains. It produced a light brown diffusible pigment, but not melanin. Comparison of genomic digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values indicated that strain SFB5A^T was related to Streptomyces litmocidini JCM 4394^T , Streptomyces …

Rationing Healthcare During A Pandemic: Shielding Healthcare Providers From Tort Liability In Uncharted Legal Territory, Frederick V. Perry, Miriam Weismann

University of Miami Business Law Review

As the coronavirus pandemic intensified, many communities in the U.S. experienced shortages of ventilators, ICU beds, and other medical supplies and treatment. There was no single national response providing guidance on the allocation of scarce healthcare resources. There has been no consistent state response either. Instead, various governmental and nongovernmental state actors in several but not all states formulated “triage protocols,” known as Crisis Standards of Care, to prioritize patient access to care where population demand exceeded supply. One intended purpose of the protocols was to immunize or shield healthcare providers from tort liability based on injuries resulting from a …

Impact Of Polygenic Risk For Coronary Artery Disease And Cardiovascular Medication Burden On Cognitive Impairment In Psychotic Disorders, Lusi Zhang, Scot Kristian Hill, Bin Guo, Baolin Wu, Ney Alliey-Rodriguez, Seenae Eum, Paulo Lizano, Elena I. Ivleva, James L. Reilly, Richard S. E. Keefe

School of Medicine Publications

Background: Cognitive impairment is a core deficit across psychotic disorders, the causes and therapeutics of which remain unclear. Epidemiological observations have suggested associations between cognitive dysfunction in psychotic disorders and cardiovascular risk factors, but an underlying etiology has not been established.

Methods: Neuropsychological performance using the Brief Assessment of Cognition in Schizophrenia (BACS) was assessed in 616 individuals of European ancestry (403 psychosis, 213 controls). Polygenic risk scores for coronary artery disease (PRSCAD) were quantified for each participant across 13 p-value thresholds (PT 0.5-5e-8). Cardiovascular and psychotropic medications were categorized for association analyses. Each PRSCAD was examined in relation to …

One Planet: One Health. A Call To Support The Initiative On A Global Science-Policy Body On Chemicals And Waste, Werner Brack, Damia Barcelo Culleres, Alistair B.A. Boxall, Helene Budzinski, Sara Castiglioni, Adrian Covaci, Valeria Dulio, Beate I. Escher, Peter Fantke, Faith Kandie, Despo Fatta-Kassinos, Felix J. Hernandez, Klara Hilscherova, Juliane Hollender, Henner Hollert, Annika Jahnke, Barbara Kasprzyk-Hordern, Stuart J. Khan, Andreas Kortenkamp, Klaus Kuemmerer, Brice Lalonde, Marja H. Lamoree, Yves Levi, Pablo Antonio Lara Martin, Cassiana C. Montagner, Christian Mougin, Titus Msagati, Joerg Oehlmann, Leo Posthuma, Malcolm Reid, Martin Reinhard, Susan D. Richardson, Et. Al.

Faculty Publications

The chemical pollution crisis severely threatens human and environmental health globally. To tackle this challenge the establishment of an overarching international science–policy body has recently been suggested. We strongly support this initiative based on the awareness that humanity has already likely left the safe operating space within planetary boundaries for novel entities including chemical pollution. Immediate action is essential and needs to be informed by sound scientific knowledge and data compiled and critically evaluated by an overarching science–policy interface body. Major challenges for such a body are (i) to foster global knowledge production on exposure, impacts and governance going beyond …

Wind And Fire: Rapid Shifts In Tree Community Composition Following Multiple Disturbances In The Southern Boreal Forest, Elias Anoszko, Lee E. Frelich, Roy L. Rich, Peter B. Reich

Aspen Bibliography

Under a warming climate, the southern boreal forest of North America is expected to see a doubling in fire frequency and potential for increased wind disturbance over the next century. Although boreal forests are often considered fire-adapted, projected increases in disturbance frequency will likely result in novel combinations of disturbances with severities and impacts on community composition outside historic norms. Using a network of repeatedly measured vegetation monitoring plots, we followed changes in tree community composition in areas of the Boundary Waters Canoe Area Wilderness (BWCAW), in Minnesota, USA, experiencing disturbances ranging from severe windstorms or wildfires to areas affected …

Functional Evolutionary Adaptation Of Binding Dynamics In The Sars-Cov-2/Ace2 Interface From Bats To Humans, Madhusudan Rajendran

Theses

The COVID-19 pandemic highlights the substantial public health, economic, and societal consequences of virus spillover from a wildlife reservoir. Widespread human transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) also presents a new set of challenges when considering viral spillover from people to naïve wildlife and other animal populations. Here, we used molecular dynamic (MD) simulations to understand the transmission of the SARS-CoV-2 virus from bats to humans and investigate the evolution of the various human variants. More specifically, we used MD simulations to understand the atomic fluctuation dampening at the receptor-binding domain (RBD)/ angiotensin-converting enzyme 2 (ACE2) interface …

Sublethal Injuries And Physical Abnormalities In Maldives Manta Rays, Mobula Alfredi And Mobula Birostris, Elspeth M. Strike, Joanna L. Harris, Kirsty L. Ballard, Julie P. Hawkins, Jennifer Crockett, Guy M.W. Stevens

School of Biological and Marine Sciences

Manta ray populations worldwide are vulnerable to sublethal injuries resulting from human activities, e.g., entanglement in fishing line and boat strikes, which have the potential to impact an individual’s health, fitness, and behaviour. Sublethal injuries and physical abnormalities also occur naturally from predation events, deformity, parasites, and disease. To determine the type and frequency of anthropogenic and natural originated injury events affecting Mobula alfredi and M. birostris in the Maldives, we examined data from the Manta Trust’s Maldivian Manta Ray Project (MMRP) database, which contains 73,638 photo-identification (photo-ID) sightings of the two manta ray species from 1987 to 2019. The …

A Review Of Camera-Trapping Methodology For Eastern Spotted Skunks, Casey G. Dukes, David S. Jachowski, Stephen N. Harris, Luke E. Dodd, Andrew J. Edelman, Summer H. Larose, Robert C. Lonsinger, Blake D. Sasse, Maximilian L. Allen

EKU Faculty and Staff Scholarship

Eastern spotted skunks Spilogale putorius are an understudied species that has experienced range-wide declines. Over the past 16 y, camera traps have become an increasingly common tool to monitor and understand their current distribution. To inform best surveying practices, we reviewed 16 camera-trap studies specifically targeting this species. We focused on reported latency to initial detection and three main aspects of study design: seasonality of detections, baits and lures, and camera-trap brands. Latency to initial detection ranged from 1 to 82 d with a mean of 17.1 d (SD = 9.1). Attractants varied among projects, but most (75%) used sardines …

Juanita Reid, Oral History Interview, 2021, Cheyenne Travioli, Matt Jones

Oral Histories

Juanita Reid served EMU for 28 years in vital roles such as Vice President of University Relations, Executive Associate to the President and Secretary of the Board of Regents. Reid's integral roles gave her a front row seat to the workings of upper administration and her view of the EMU logo and mascot change reflects this close, professional proximity. Explaining the rationale of the Board of Regents and the president in changing the mascot and logo, as well as the reactions from the community both on and off campus, Reid paints a uniquely vivid portrait of a university enmeshed in …

Gardner-Webb Biology Honor Society Inducts New Members, Office Of University Communications

Gardner-Webb NewsCenter Archive

Beta Beta Beta (Tri-Beta) Biology Honor Society held its induction ceremony on March 2. The society is dedicated to the understanding and appreciation of the biological sciences.

Multi-Year Data Analysis And Genomic Selection To Improve The Efficiency Of A Rice Breeding Program, Tommaso Cerioli

LSU Doctoral Dissertations

Plant breeding dramatically improved crops performances during human history and will play a pivotal role in shaping the future of agriculture. However, this activity requires large amount of time and resources. The availability of multi-year datasets and abundant DNA information enables new analyses and breeding approaches that can increase the productivity and efficiency of a breeding program. In recent years, the LSU Rice Breeding Program has implemented marker assisted selection (MAS) and genomic selection (GS) to predict performance of rice genotypes before field testing. The goal of this project was to conduct analyses and test molecular approaches to increase the …

Dose-Finding Study Of Ibrutinib And Venetoclax In Relapsed Or Refractory Mantle Cell Lymphoma, Craig A Portell, Nolan A Wages, Brad S Kahl, Lihua E Budde, Robert W Chen, Jonathon B Cohen, Nikole E Varhegyi, Gina R Petroni, Michael E Williams

Open Access Publications

Relapsed Mantle cell lymphoma (MCL) is often treated with Bruton's tyrosine kinase inhibitors (BTKi); however, post-BTKi relapse can be challenging. Adding venetoclax (VEN) to ibrutinib (IBR) has shown synergy in preclinical MCL models. Prior MCL studies of the combination show promising efficacy but have conducted limited dose finding. We sought to identify the optimal dosing combination, based on efficacy and toxicity, utilizing a continual reassessment method of 6 combinations of IBR (280 mg, 420 mg, and 560 mg by mouth daily) and VEN (max dose of 200 mg and 400 mg by mouth daily). Eligible participants were not previously exposed …

Engineered Fgf1 And Fgf2 Compositions And Methods Of Use Thereof, Thallapuranam Krishnaswamy Suresh Kumar, Shilpi Agarwal, Ravi Kumar Gundampati, Srinivas Jayanthi, Tengjiao Wang, Jake Jones, Olivia Kolenc, Ngoc Thien Lam, Isabelle Ishimwe Niyonshuti, Kartik Balachandran, Kyle Quinn, Jingyi Chen

Patents Granted

Engineered FGF1 and FGF2 polypeptides, polynucleotides encoding these polypeptides and DNA constructs, vectors and compositions including these engineered polypeptides are provided herein. The engineered FGF1 and FGF2 polypeptides are more stable than their wild-type counterparts and may be more effective at treating a variety of conditions that FGF1 and FGF2 are useful for treating such as wound healing.

Mapping Bacterial Biofilm On Features Of Orthopedic Implants In Vitro, Kelly Moore, Niraj Gupta, Tripti Thapa Gupta, Khushi Patel, Jacob R. Brooks, Anne Sullivan, Alan S. Litsky, Paul Stoodley

Publications

Implant-associated infection is a major complication of orthopedic surgery. One of the most common organisms identified in periprosthetic joint infections is Staphylococcus aureus, a biofilm-forming pathogen. Orthopedic implants are composed of a variety of materials, such as titanium, polyethylene and stainless steel, which are at risk for colonization by bacterial biofilms. Little is known about how larger surface features of orthopedic hardware (such as ridges, holes, edges, etc.) influence biofilm formation and attachment. To study how biofilms might form on actual components, we submerged multiple orthopedic implants of various shapes, sizes, roughness and material type in brain heart infusion broth …

Barriers To Mental Health Help-Seeking Among The Latinx Population In The United States: Primary Care, Family Influences, And Cultural Stigmas, Taylor Percival

Undergraduate Honors Theses

This thesis explores the disparity between the incidence of mental illness and help-seeking and resource utilization among the Latinx population. Research supports that the incidence of mental health issues is as high among this population as it is among any other, but help-seeking behaviors and resource utilization among this population are significantly lower. It is proposed that this difference is caused by family influence, cultural stigma, and lack of access to education and resources. Based on these factors, this thesis will discuss potential solutions that are culturally appropriate and empirically supported. These include family support interventions (psychoeducation, family-centered treatments, and …

Differential Recognition Of Canonical Nf-Κb Dimers By Importin Α3, Tyler J. Florio, Ravi K Lokareddy, Daniel P Yeggoni, Rajeshwer S Sankhala, Connor A Ott, Richard E Gillilan, Gino Cingolani

Department of Biochemistry and Molecular Biology Faculty Papers

Nuclear translocation of the p50/p65 heterodimer is essential for NF-κB signaling. In unstimulated cells, p50/p65 is retained by the inhibitor IκBα in the cytoplasm that masks the p65-nuclear localization sequence (NLS). Upon activation, p50/p65 is translocated into the nucleus by the adapter importin α3 and the receptor importin β. Here, we describe a bipartite NLS in p50/p65, analogous to nucleoplasmin NLS but exposed in trans. Importin α3 accommodates the p50- and p65-NLSs at the major and minor NLS-binding pockets, respectively. The p50-NLS is the predominant binding determinant, while the p65-NLS induces a conformational change in the Armadillo 7 of importin …

Cluttering In The Speech Of Young Men With Fragile X Syndrome, Katherine Bangert, Kathleen Scaler Scott, Charley Adams, Jessica S. Kisenwether, Lisa Giuffre, Jenna Reed, Angela John Thurman, Leonard Abbeduto, Jessica Klusek

Faculty Publications

Purpose: Cluttering is a fluency disorder that has been noted clinically in individuals with fragile X syndrome (FXS). Yet, cluttering has not been systematically characterized in this population, hindering identification and intervention efforts. This study examined the rates of cluttering in male young adults with FXS using expert clinical opinion, the alignment between expert clinical opinion and objectively quantified features of cluttering from language transcripts, and the association between cluttering and aspects of the FXS phenotype.

Method: Thirty-six men with FXS (aged 18-26 years; M = 22, SD = 2.35) contributed language samples and completed measures of nonverbal cognition, autism …

Analysis Of The Circrna And T-Ucr Populations Identifies Convergent Pathways In Mouse And Human Models Of Rett Syndrome, Edilene Siqueira, Aida Obiols-Guardia, Olga C Jorge-Torres, Cristina Oliveira-Mateos, Marta Soler, Deepthi Ramesh-Kumar, Fernando Setién, Daniëlle Van Rossum, Ainhoa Pascual-Alonso, Clara Xiol, Cristina Ivan, Masayoshi Shimizu, Judith Armstrong, George A Calin, R Jeroen Pasterkamp, Manel Esteller, Sonia Guil

Faculty, Staff and Student Publications

Noncoding RNAs play regulatory roles in physiopathology, but their involvement in neurodevelopmental diseases is poorly understood. Rett syndrome is a severe, progressive neurodevelopmental disorder linked to loss-of-function mutations of the MeCP2 gene for which no cure is yet available. Analysis of the noncoding RNA profile corresponding to the brain-abundant circular RNA (circRNA) and transcribed-ultraconserved region (T-UCR) populations in a mouse model of the disease reveals widespread dysregulation and enrichment in glutamatergic excitatory signaling and microtubule cytoskeleton pathways of the corresponding host genes. Proteomic analysis of hippocampal samples from affected individuals confirms abnormal levels of several cytoskeleton-related proteins together with key …

Identification Of The Global Mir-130a Targetome Reveals A Role For Tbl1xr1 In Hematopoietic Stem Cell Self-Renewal And T(8; 21) Aml, Gabriela Krivdova, Veronique Voisin, Erwin M Schoof, Sajid A Marhon, Alex Murison, Jessica L Mcleod, Martino M Gabra, Andy G X Zeng, Stefan Aigner, Brian A Yee, Alexander A Shishkin, Eric L Van Nostrand, Karin G Hermans, Aaron C Trotman-Grant, Nathan Mbong, James A Kennedy, Olga I Gan, Elvin Wagenblast, Daniel D De Carvalho, Leonardo Salmena, Mark D Minden, Gary D Bader, Gene W Yeo, John E Dick, Eric R Lechman

Faculty, Staff and Students Publications

Gene expression profiling and proteome analysis of normal and malignant hematopoietic stem cells (HSCs) point to shared core stemness properties. However, discordance between mRNA and protein signatures highlights an important role for post-transcriptional regulation by microRNAs (miRNAs) in governing this critical nexus. Here, we identify miR-130a as a regulator of HSC self-renewal and differentiation. Enforced expression of miR-130a impairs B lymphoid differentiation and expands long-term HSCs. Integration of protein mass spectrometry and chimeric AGO2 crosslinking and immunoprecipitation (CLIP) identifies TBL1XR1 as a primary miR-130a target, whose loss of function phenocopies miR-130a overexpression. Moreover, we report that miR-130a is highly expressed …

Defining The Mammalian Coactivation Of Hepatic 12-H Clock And Lipid Metabolism, Huan Meng, Naomi M Gonzales, Sung Yun Jung, Yue Lu, Nagireddy Putluri, Bokai Zhu, Clifford C Dacso, David M Lonard, Bert W O'Malley

Faculty, Staff and Students Publications

The 12-h clock coordinates lipid homeostasis, energy metabolism, and stress rhythms via the transcriptional regulator XBP1. However, the biochemical and physiological bases for integrated control of the 12-h clock and diverse metabolic pathways remain unclear. Here, we show that steroid receptor coactivator SRC-3 coactivates XBP1 transcription and regulates hepatic 12-h cistrome and gene rhythmicity. Mice lacking SRC-3 show abnormal 12-h rhythms in hepatic transcription, metabolic functions, systemic energetics, and rate-limiting lipid metabolic processes, including triglyceride, phospholipid, and cardiolipin pathways. Notably, 12-h clock coactivation is not only preserved, with its cistromic activation priming ahead of the zeitgeber cue of light, but …

Genetically Based Adaptive Trait Shifts At An Expanding Mangrove Range Margin, John Paul Kennedy, Giles N. Johnson, Richard F. Preziosi, Jennifer K. Rowntree

School of Biological and Marine Sciences

AbstractNeotropical black mangrove (Avicennia germinans) is expanding poleward into temperate salt marsh along Atlantic Florida, USA, with field evidence of trait shifts consistent with greater cold tolerance within range margin populations. However, whether these shifts have a genetic basis remains unanswered. To address this gap, we measured multiple phenotypic traits of twenty A. germinans maternal cohorts from areas in both the Atlantic Florida range core and margin in a 2-year greenhouse common garden with annual temperatures analogous to range margin conditions. Compared to those from the range core, range margin cohorts survived in greater numbers, established (i.e., produced first true …

Structural And Computational Studies Of The Sars-Cov-2 Spike Protein Binding Mechanisms With Nanobodies: From Structure And Dynamics To Avidity-Driven Nanobody Engineering, Gennady M. Verkhivker

Mathematics, Physics, and Computer Science Faculty Articles and Research

Nanobodies provide important advantages over traditional antibodies, including their smaller size and robust biochemical properties such as high thermal stability, high solubility, and the ability to be bioengineered into novel multivalent, multi-specific, and high-affinity molecules, making them a class of emerging powerful therapies against SARS-CoV-2. Recent research efforts on the design, protein engineering, and structure-functional characterization of nanobodies and their binding with SARS-CoV-2 S proteins reflected a growing realization that nanobody combinations can exploit distinct binding epitopes and leverage the intrinsic plasticity of the conformational landscape for the SARS-CoV-2 S protein to produce efficient neutralizing and mutation resistant characteristics. Structural …

Intergenerational Risk And Resilience Pathways From Discrimination And Acculturative Stress To Infant Mental Health, Sabrina R. Liu, Curt A. Sandman, Elyssia Poggi Davis, Laura M. Glynn

Psychology Faculty Articles and Research

Preconception and prenatal stress impact fetal and infant development, and women of color are disproportionately exposed to sociocultural stressors like discrimination and acculturative stress. However, few studies examine links between mothers’ exposure to these stressors and offspring mental health, or possible mitigating factors. Using linear regression, we tested associations between prenatally assessed maternal acculturative stress and discrimination on infant negative emotionality among 113 Latinx/Hispanic, Asian American, Black, and Multiethnic mothers and their children. Additionally, we tested interactions between stressors and potential pre- and postnatal resilience-promoting factors: community cohesion, social support, communalism, and parenting self-efficacy. Discrimination and acculturative stress were related …

The Relationship Between Perceived Health Outcomes Of Recreation, Exercise Dependence, And Life Satisfaction: A Study With Fitness Center Members, Emrah Serdar

Baltic Journal of Health and Physical Activity

Background: This study aimed to investigate the relationship between perceived health outcomes of recreation, exercise dependence, and life satisfaction among fitness center members. Material and methods: The study sample consisted of 490 participants, 336 males, and 154 females, who were members of a private fitness center in Istanbul. The participants were selected using a purposeful sampling method. Data collection tools included the Perceived Health Outcomes of Recreation Scale, developed by Gomez et al. and adapted to Turkish by Yerlisu Lapa et al., the Exercise Dependence-21 Scale, developed by Hausenblans and Downs and adapted by Gürbüz and Aşçı and the Life …

The Investigation Of The Relationship Between Perceived Freedom And Brief Resilience: The Role Of Participation In Physical Activity, Tülay Avcı Taşkıran, Bülent Gürbüz

Baltic Journal of Health and Physical Activity

Background: This study was designed to examine the relationship between perceived freedom in leisure and psychological resilience of university staff. Material and Methods: The sample group of the study was composed of 189 academic and 81 administrative university staff (Mage=36.11±7.34). Participants were assessed based on the “Perceived Freedom in Leisure Scale (T-PFLS)” and the “Brief Resilience Scale (T-BRS)”. ANOVA, independent samples t-test, and Pearson correlation analysis methods were used to analyze the data. Results: T-test analysis indicated that there was no significant difference between the mean scores of “T-PFLS”; however, there was a statistically significant difference between the mean scores …

Too Masculine For Healthcare, Too Feminine For Intense Sports: Correlation Between Gender Conformity And Pro-Health Behaviours, Aleksandra Pawlowska, Kornelia Lipowska, Daniel Krokosz

Baltic Journal of Health and Physical Activity

Background: The goal of this paper is to examine to what extent levels of femininity and masculinity are associated with practicing several types of health behaviours among individuals who are physically active and inactive. Material/Methods: A total of 559 individuals aged between 35 and 45 (M = 39.4; SD = 3.2) took part in the study. The inactive group consisted of 211 individuals (n = 85 women, n = 126 men), and the active group consisted of 348 individuals (n = 131 women, n = 217 men). The participants completed the following pen-and-paper questionnaires: the Psychological Gender Inventory, the Health …

Addressing Poverty, Children’S Mental Health, And Education; Coming Together As A Community, Jim C. Taylor Mr.

National Youth Advocacy & Resilience Conference

Existing within every society are various cultures and sub-cultures. This presentation examines the relationship between poverty, children’s mental health, and education. We will review existing literature and practices designed to address these issues both in and out of the classroom and discuss what we as a community can do to minimize the effects of poverty and mental health and maximize successful education.

Proceedings Of The 20th Annual Urban Ecology & Conservation Symposium (2022), Urban Ecosystem Research Consortium Of Portland/Vancouver

Urban Ecosystem Research Consortium of Portland/Vancouver

These proceedings document the agenda and abstracts for presentions and posters from the annual conference organized by the Urban Ecosystem Research Consortium of Portland/Vancouver (UERC). The UERC is a consortium of people from various universities and colleges, state and federal agencies, local governments, non-profit organizations and independent professionals interested in supporting urban ecosystem research and creating an information-sharing network of people that collect and use ecological data in the Portland/Vancouver area. The annual conference has an open call for abstracts. The UERC organizing committee selects the final oral and poster presentations, along with 2 invited keynote presentations.

Taking The "Fam" Out Of Family: Adjudicating The State Department's Discriminatory Treatment Of Same-Sex Parents On The Merits, Camrin M. Rivera

Maine Law Review

Cisgender same-sex male married couples, unlike cisgender opposite-sex married couples, will always require artificial reproductive technology (ART) for at least one of the spouses to attain biological parenthood. Due to legal and financial barriers to ART, many of these couples turn to international ART services to grow their families. In doing so, these families may face immigration battles when they apply for recognition of their child’s United States citizenship. For example, a prior State Department policy sparked three lawsuits after the State Department refused to recognize children as United States citizens from birth because the children were not biologically related …