Digital Commons Network^™

Prmt5 Interacting Partners And Substrates In Oligodendrocyte Lineage Cells, David K. Dansu, Jialiang Liang, Ipek Selcen, Haiyan Zheng, Dirk F. Moore, Patricia Casaccia

Advanced Science Research Center

The protein arginine methyl transferase PRMT5 is an enzyme expressed in oligodendrocyte lineage cells and responsible for the symmetric methylation of arginine residues on histone tails. Previous work from our laboratory identified PRMT5 as critical for myelination, due to its transcriptional regulation of genes involved in survival and early stages of differentiation. However, besides its nuclear localization, PRMT5 is found at high levels in the cytoplasm of several cell types, including oligodendrocyte progenitor cells (OPCs) and yet, its interacting partners in this lineage, remain elusive. By using mass spectrometry on protein eluates from extracts generated from primary oligodendrocyte lineage cells …

A Qualitative Inquiry Of The Body Project On College Campuses In Illinois, Maria Ross

Theses and Dissertations

Negative body image is a social and psychological concern that plagues many young women in the United States and has a strong relationship to the onset of eating disorders. One outcome of past research done on body dissatisfaction and eating concerns has been the implementation of peer-led prevention programs such as The Body Project and More Than Muscles that address negative body image and disordered eating. These programs target the sociocultural factors that contribute to eating and body image issues on college campuses. This study uses interviews supplemented by ethnographic fieldnotes and content analysis to describe and evaluate the implementation …

Proteomic Profiling Based Classification Of Cll Provides Prognostication For Modern Therapy And Identifies Novel Therapeutic Targets, Ti'ara L Griffen, Fieke W Hoff, Yihua Qiu, James W Lillard, Alessandra Ferrajoli, Philip Thompson, Endurance Toro, Kevin Ruiz, Jan Burger, William Wierda, Steven M Kornblau

Faculty, Staff and Student Publications

Protein expression for 384 total and post-translationally modified proteins was assessed in 871 CLL and MSBL patients and was integrated with clinical data to identify strategies for improving diagnostics and therapy, making this the largest CLL proteomics study to date. Proteomics identified six recurrent signatures that were highly prognostic of survival and time to first or second treatment at three levels: individual proteins, when grouped into 40 functionally related groups (PFGs), and systemically in signatures (SGs). A novel SG characterized by hairy cell leukemia like proteomics but poor therapy response was discovered. SG membership superseded other prognostic factors (Rai Staging, …

Techno-Economic And Sustainability Analysis Of Renewable Natural Gas (Rng) Production, Eesha Bhattacharjee

USF Tampa Graduate Theses and Dissertations

Natural Gas (NG) is one of the major sources of energy currently used in the United States. Its major applications include generating electricity, domestic, and industrial heating purposes and as a transportation fuel. The largest component of NG is methane, and it contains trace amounts of hydrocarbons, some CO2 and water vapor.

Biogas (produced by anaerobic digestion (AD) of biomass) upgraded to have high purity methane (often termed as biomethane or renewable natural gas) can be used as an alternative to NG. The upgrading techniques may include CO2 separation from the biogas stream or via to a methanation facility where …

Asphaltene Or Polyvinylchloride Waste Blended With Cement To Produce A Sustainable Material Used In Nuclear Safety, Heba A. Esawii, Hosam M. Saleh, Ibrahim I. Bondouk, Elsayed Salama

Faculty Journal Articles

The current research uses sustainable methods to preserve the environment, such as exploiting municipal or industrial waste that may harm the environment. The wreckage of polyvinyl chloride (PVC) pipes and asphaltene are used as additives to cement to improve its mechanical properties, while stabilizing the radioactive waste resulting from the peaceful uses of nuclear materials, or enhancing its radiation shielding efficiency. New composites of Portland cement with ground PVC or asphaltene up to 50% are investigated. Fast neutron removal cross-section (ƩR) and gamma shielding parameters, such as mass attenuation coefficient (MAC), half-value layer (HVL), effective atomic number (Zeff), and exposure …

Acute And Chronic Bone Marker And Endocrine Responses To Resistance Exercise With And Without Blood Flow Restriction In Young Men, Debra A. Bemben, Vanessa D. Sherk, Samuel R. Buchanan, Sojung Kim, Kyle Sherk, Michael G. Bemben

Health & Human Performance Faculty Publications

In this study, we compared acute and chronic bone marker and hormone responses to 6 weeks of low intensity (20% 1RM) blood flow restriction (BFR20) resistance training to high intensity (70% 1RM) traditional resistance training (TR70) and moderate intensity (45% 1RM) traditional resistance training (TR45) in young men (18–35 years). Participants were randomized to one of the training groups or to a control group (CON). The following training programs were performed 3 days per week for 6 weeks for knee extension and knee flexion exercises: BFR20, 20%1RM, 4 sets (30, 15, 15, 15 reps) wearing blood flow restriction cuffs around …

A Non-Invasive Interpretable Diagnosis Of Melanoma Skin Cancer Using Deep Learning And Ensemble Stacking Of Machine Learning Models, Iftiaz A. Alfi, Mahfuzur Rahman, Mohammad Shorfuzzaman, Amril Nazir

All Works

A skin lesion is a portion of skin that observes abnormal growth compared to other areas of the skin. The ISIC 2018 lesion dataset has seven classes. A miniature dataset version of it is also available with only two classes: malignant and benign. Malignant tumors are tumors that are cancerous, and benign tumors are non-cancerous. Malignant tumors have the ability to multiply and spread throughout the body at a much faster rate. The early detection of the cancerous skin lesion is crucial for the survival of the patient. Deep learning models and machine learning models play an essential role in …

Amphiphilic Cyclic Peptide [W4Kr5]-Antibiotics Combinations As Broad-Spectrum Antimicrobial Agents, Eman H. M. Mohammed, Sandeep Lohan, Rakesh K. Tiwari, Keykavous Parang

Pharmacy Faculty Articles and Research

Linear and cyclic amphiphilic peptides, (W₄KR₅) and [W₄KR₅], were evaluated as antibacterial agents against Gram-positive and Gram-negative bacteria, including four multi-drug resistant strains and the corresponding four non-resistant strains. Cyclic peptide [W₄KR₅] showed higher antibacterial activity than the linear (W₄KR₅) counterpart. Cyclic [W₄KR₅] was subjected to combination (physical mixture or covalent conjugation) with meropenem as a model antibiotic to study the impact of the combination on antimicrobial activity. A physical mixture of meropenem and [W₄KR₅] showed …

The Therapeutic Effect Of Exogenous Melatonin On Depressive Symptoms: A Systematic Review And Meta-Analysis, Cancan Li, Dandan Ma, Min Li, Tao Wei, Xuan Zhao, Yuanyuan Heng, Delong Ma, Enoch Odame Anto, Yanbo Zhang, Mingyun Niu, Wangxin Zhang

Research outputs 2022 to 2026

Background: Depression-related mortality and morbidity pose growing public health burdens worldwide. Although the therapeutic effect of exogenous melatonin on depression has been investigated, findings remain inconsistent. We conducted this systematic review and meta-analysis to clarify the effectiveness of melatonin in the treatment of depression, including primary and secondary depression symptoms. Methods: We searched the online databases of PubMed, EMBASE, and the Cochrane Library for original studies published up to May 2021. We used STATA 14.0 software to synthesize the results of included studies. To evaluate the effectiveness of melatonin, we calculated the standardized mean differences (SMDs) and 95% confidence intervals …

How A 7-Week Food Literacy Cooking Program Affects Cooking Confidence And Mental Health: Findings Of A Quasi-Experimental Controlled Intervention Trial, Joanna Rees, Shih Ching Fu, Johnny Lo, Ros Sambell, Joshua R. Lewis, Claus T. Christophersen, Matthew F. Byrne, Robert U. Newton, Siobhan Boyle, Amanda Devine

Research outputs 2022 to 2026

Obesity and mental health disorders are rising simultaneously with shifting dietary behavior away from home cooking, toward typically nutrition-poor and energy-dense convenience meals. Food literacy strongly influences nutrition choices. Community-based cooking interventions target barriers to healthy eating and facilitate development of food literacy skills, thereby potentially increasing preparation of home-cooked meals and positively influencing health. This study of 657 healthy Australian adults explored the efficacy of a 7-week cooking program in improving cooking confidence, whether this transferred to behavior surrounding food, and/or affected mental health. Significant post-program improvements in cooking confidence and satisfaction (all p < 0.001, (Formula presented.) 1.12 large), ability to change eating habits (p < 0.001) and overcome lifestyle barriers (p = 0.005) were observed for the intervention group but not control. Participation also improved mental and general health (all p < 0.05, (Formula presented.) 0.02 small). No changes were observed for acquisition and consumption of food, or nutrition knowledge in either group. This 7-week cooking program built cooking confidence and improved general and mental health but did not change dietary behavior. To further improve nutrition related behaviors associated with better mental health, more effort is needed to recruit those with below-average nutrition knowledge and interest in cooking.

Parole Interview Transcript/Decision - Fusl000112 (2019-10-21)

Parole Interview Transcripts and Decisions

No abstract provided.

How Graduate Student Fellows Enhance What A Center For Digital Scholarship Does, Ben B. Chiewphasa

Transforming Libraries for Graduate Students

Multiple disciplines are increasingly embracing data science and digital scholarship. However, insufficient training for digital and computational methodologies within subject/departmental silos means that these needs often get overlooked. Opportunities for learning how to teach technical concepts (i.e., how to handle troubleshooting, live participatory coding, etc.) are also rare or non-existent via departmental offerings. To respond to these needs, the Navari Family Center for Digital Scholarship launched its Pedagogy Fellowship Program in Fall 2021 where Notre Dame PhD students/candidates build their instructional expertise and experience related to digital scholarship with an added bonus of enhancing their competitiveness on the job market. …

Repurposing Lansoprazole And Posaconazole To Treat Leishmaniasis: Integration Of In Vitro Testing, Pharmacological Corroboration, And Mechanisms Of Action, Yash Gupta, Steven Goicoechea, Jesus G. Romero, Raman Mathur, Thomas R. Caulfield, Daniel P. Becker, Ravi Durvasula, Prakasha Kempaiah

Journal of Food and Drug Analysis

Leishmaniasis remains a serious public health problem in many tropical regions of the world. Among neglected tropical diseases, the mortality rate of leishmaniasis is second only to malaria. All currently approved therapeutics have toxic side effects and face rapidly increasing resistance. To identify existing drugs with antileishmanial activity and predict the mechanism of action, we designed a drug-discovery pipeline utilizing both in-silico and in-vitro methods. First, we screened compounds from the Selleckchem Bio-Active Compound Library containing ~1,622 FDA-approved drugs and narrowed these down to 96 candidates based on data mining for possible anti-parasitic properties. Next, we completed preliminary in-vitro testing …

A Three-Step Stacking Capillary Electrophoresis Of Field-Amplified Sample Injection, Sweeping, And Micellar Collapse For Determination Of Dabigatran And Its Active Metabolite In Human Plasma, Cheng-Wei Cheng, Wen-Han Feng, Wei-Hua Tang, Hwang-Shang Kou, Chun-Chi Wang, Shou-Mei Wu

Journal of Food and Drug Analysis

A three-step stacking capillary electrophoresis (CE) composed of field-amplified sample injection, sweeping, and analyte focusing by micellar collapse (FASI-sweeping-AFMC) was developed to determine dabigatran (D) and its major active metabolite, dabigatran acyl-beta-d-glucuronide (DAG), in human plasma. After optimization and validation, this novel approach was further applied to monitor 5 real samples, and the 25.2 to 186.8 ng mL^-1 D could be observed among those. Based on these results, the novel CE stacking strategy was successfully applied for the analysis of D and DAG in human plasma and could be served as a tool for clinical assays.

The Antihyperlipidemic Equivalent Combinatorial Components From Peel Of Citrus Reticulata ‘Chachi’, Ping-Ting Xiao, Yu-Jia Kuang, Shi-Yu Liu, Zhi-Shen Xie, Jin-Hua Hao, E-Hu Liu

Journal of Food and Drug Analysis

Since the combinatorial components responsible for the antihyperlipidemic activity of Citrus reticulata‘Chachi’ (CRC) peels remains unclear, we herein developed a bioactive equivalence oriented feedback screening method to discover the bioactive equivalent combinatorial components (BECCs) from CRC peels. Using palmitic acid (PA)- stimulated hepatocyte model, a combination of five polymethoxyflavones (PMFs) including tangeretin, sinensetin, nobiletin, 5,7,8,4′-tetramethoxyflavone and 3,5,6,7,8,3',4'-heptamethoxyflavone was identified to be responsible for the antihyperlipidemic effect of CRC peels. Via evaluation of combination effect by combination index (CI), these 5 PMFs were found to take effect via a synergistic mode. Our data indicated that the antihyperlipidemic mechanism of PMF …

Food Glycomics: Dealing With Unexpected Degradation Of Oligosaccharides During Sample Preparation And Analysis, Yu-Ping Huang, Randall C. Robinson, Daniela Barile

Journal of Food and Drug Analysis

This study reveals that unexpected degradation of food oligosaccharides can occur during conventional glycomics workflows, including sample preparation and analysis by liquid chromatography-mass spectrometry (LC-MS). With the present investigation, we aim to alert the scientific community of the susceptibility of specific glycosidic linkages to degradation induced by heat and acid. Key standard oligosaccharides representing the major types found in foods (3’-sialyllactose and 6’-sialyl-N-acetyllactosamine for milk, raffinose and stachyose for legumes) were selected as model systems and underwent each of the following treatments independently: (1) labeled with the derivatizing agent 1-aminopyrene-3,6,8-trisulfonic (APTS) (followed by analysis with a capillary electrophoresis system coupled …

Ulva Prolifera Polysaccharide Exerts Anti-Obesity Effects Via Upregulation Of Adiponectin Expression And Gut Microbiota Modulation In High-Fat Diet-Fed C57bl/6 Mice, Hai-Ching Pung, Wei-Sheng Lin, Yi-Chen Lo, Cheng-Chih Hsu, Chi-Tang Ho, Min-Hsiung Pan

Journal of Food and Drug Analysis

Obesity is characterized by metabolic disorder and accompanying an altered and less diverse gut microbiota composition during a fat-enriched diet. Recent studies indicated that sulphated polysaccharide prevents high-fat diet (HFD) induced obesity, reduces metabolic disorder, and restores the gut microbiota. However, there are few studies about Ulva prolifera polysaccharide (UPP) may induce anti-obesogenic effects. Therefore, the present study investigates the enzymatic extracted UPP effects in HFD-fed mice. The results showed that UPP considerably slowed down the HFD-induced weight gain and improved metabolic disorders in HFD-fed mice. Notably, the effects were associated with lower body weight gain, reduced adipose tissue hypertrophy, …

Direct Immersion Solid-Phase Microextraction Combined With Ambient Ionization Tandem Mass Spectrometry To Rapidly Distinguish Pesticides In Serum For Emergency Diagnostics, Hung Su, Hsin-Hua Lin, Lin-Jhen Su, Chia-Chen Lin, Zong-Han Jiang, Shiang-Jin Chen, Jentaie Shiea, Chi-Wei Lee

Journal of Food and Drug Analysis

Despite the fact that carbamates and organophosphates cause acute poisoning via different mechanisms and require disparate management, they are indistinguishable by current clinical assays. Herein, direct immersion solid-phase microextraction (DI-SPME) plus thermal desorption-electrospray ionization tandem mass spectrometry (TD-ESI/MS/MS) was developed to discern them. Both pesticides spiked in human serum were extracted by SPME and analyzed by TD-ESI/MS/MS. This is a promising emergency care platform as rapid analyses could be done in tiny sample volumes with satisfactory recovery (89.46%–116.32%), precision (covariance < 20%), sensitivity (LOD < 0.1 µg/mL), turnaround time (< 5 minutes), and linearity (R² = 0.9827–0.9992) within 0.1–100 µg/mL.

The Next Generation Beneficial Actions Of Novel Probiotics As Potential Therapeutic Targets And Prediction Tool For Metabolic Diseases, Hsin-Lin Cheng, Gow-Chin Yen, Shih-Chien Huang, Shiuan-Chih Chen, Chin-Lin Hsu

Journal of Food and Drug Analysis

The prevalence of metabolic disease has rising and affected over 1,000 million populations globally. Since the metabolic disease and its related complication are board, it has become the major health hazard of modern world. However, Long term medication of metabolic disease may cause serious side effects and risk for adverse health problems. Recently, emerging studies focus on exploring the mechanistic details of metabolic state in disease development and progression. Gut bacteria ecosystem was considered to play a pivotal role in regulating energy homeostasis and great associated with the development of metabolic disease. Accumulated evidences indicated that Akkermansia muciniphila, Faecalibacterium prausnitzii, …

Protection Of Karst Features During Project Development And Construction, Sandra Bowman, Jessica Eichhorst, Kia Gillette

Purdue Road School

INDOT released a new procedure in July 2021 to protect karst features during project development and construction. Three key components of the new procedure are: (1) an expanded Indiana Karst Region, (2) integration with other INDOT environmental procedures and federal and state environmental laws, and (3) an educational component that includes best management practices and karst geology and biology. This presentation will provide an overview of the procedure, implementation guidance, and key concepts.

The Relation Between Selected Anthropometric Variables And The Sports Results Of Early Pubescent Swimmers, Grzegorz Bielec, Daniel Jurak

Baltic Journal of Health and Physical Activity

Background: Relationships between anthropometric parameters and sports results in swimming are well documented, but only few studies concern young athletes. Scientific evidence does not indicate definitely which anthropometric variables determine swimming performance among pubertal athletes. Material and methods: Forty-one swimmers at the mean age of 12.2 years (including 15 females) were examined. Body composition was assessed with a Tanita BC 545N device. Body height, hand length, hand width and arm span were also measured. Anthropometric data were compared to results of 50 m freestyle and 200 m individual medley. Results: Calculations made by Pearson’s coefficient revealed significant correlations between body …

Physical Activity Of Working Age Wroclaw Residents With Reference To Their Income, Daniel Puciato, Michal Rozpara, Piotr Olesniewicz, Julita Markiewicz-Patkowska, Soňa Jandová

Baltic Journal of Health and Physical Activity

Background: ‪The aim of the paper was to assess the relationship between physical activity and the material status in working age residents of Wrocław, Poland. Material and methods: ‪The research covered 3,495 people aged 18–65 years. The research tool was the short version of the IPAQ survey questionnaire. The following information was obtained: age, sex, height, weight, and gross monthly income per capita in the household. Basic statistical characteristics were calculated, as well as the Mann-Whitney test for samples with n > 20 and the Kruskal-Wallis ANOVA test. Statistical significance was assumed for p < 0.05. Results: ‪In the weekly physical activity structure of the surveyed Wrocław residents, low-intensity activity was dominant. Usually, men were more engaged in physical activity of high intensity than women; women turned out more physically active than men within the range of low-intensity exercise. The gross monthly income per capita differentiated the categories of overall, moderate-intensity, and low-intensity physical activity in the surveyed women, and those of overall and low-intensity physical activity in the surveyed men. In both sexes, respondents with low income were characterised by the lowest physical activity. Women with medium income and men with high income were the most physically active. Conclusions: ‪It seems necessary to include the poorest working age people in public health programs aimed at increasing the level of physical activity.

A Preparation Cycle In Strength Training As A Form Of Health Training For Adults, Malgorzata Kalwa, Tadeusz Stefaniak, Dariusz Harmacinski, Bartosz Groffik

Baltic Journal of Health and Physical Activity

Background: ‪The authors assume that 8-month systematic physical activity with individually selected resistance of progressively increasing volume and intensity will counteract the processes related to regress of motricity and the unfavourable change of body composition in people over 40 years old. Material and methods: ‪The research involved 178 persons (92 women and 86 men) aged 40–61 years old. The participants pursued training with small and medium weights, broken down into a younger (40–50 years) and an older (51–61 years) group. The workout plan was modelled on the adaptive strength training method (ASTM), which is used by weightlifters and bodybuilders. The …

Climate Change, Air Pollution, And Children’S Mental Health, Wuyue Yu, Michael Weitzman

Journal of Applied Research on Children: Informing Policy for Children at Risk

Climate change and air pollution are twin global public health challenges of high priority. Sharing linked causes and solutions, both climate change and air pollution are suggested by a growing body of research to inflict negative mental health outcomes through multiple mechanisms. However, there are still gaps in understanding the links between climate change, air pollution and mental health, especially in children and adolescents, who are particularly at-risk. Current findings are summarized and their implications for future research as well as public health policies are discussed.

Delineating The Exocytic Fusion Machinery Required For Mast Cell Exocytosis, Pratikshya Adhikari

Dissertations

Mast cells undergo exocytosis to release a wide array of inflammatory mediators by utilizing membrane fusion proteins-SNAREs (soluble-N-ethyl-maleimide sensitive factor attachment protein receptor) along with essential regulatory Munc18 and Munc13 proteins. Accumulating evidence in mast cell biology suggests the existence of distinct pools of mast cell mediators. However, the precise mechanism underlying the release of each mast cell mediator is not clear. To determine whether different exocytic machineries are required for differential mediator release, I used reconstitution to investigate the differential role of Munc18s in fusion machinery regulation. Munc18a and Munc18c stimulated VAMP2 and VAMP3-mediated lipid mixing, whereas Munc18b only …

Deep Learning Approach For Chemistry And Processing History Prediction From Materials Microstructure, Amir Abbas Kazemzadeh Farizhandi, Omar Betancourt, Mahmood Mamivand

Mechanical and Biomedical Engineering Faculty Publications and Presentations

Finding the chemical composition and processing history from a microstructure morphology for heterogeneous materials is desired in many applications. While the simulation methods based on physical concepts such as the phase-field method can predict the spatio-temporal evolution of the materials’ microstructure, they are not efficient techniques for predicting processing and chemistry if a specific morphology is desired. In this study, we propose a framework based on a deep learning approach that enables us to predict the chemistry and processing history just by reading the morphological distribution of one element. As a case study, we used a dataset from spinodal decomposition …

Benthic Biolayer Structure Controls Whole-Stream Reactive Transport, Kevin R. Roche, Marco Dentz

Civil Engineering Faculty Publications and Presentations

Hyporheic zone reaction rates are highest just below the sediment-water interface, in a shallow region called the benthic biolayer. Vertical variability of hyporheic reaction rates leads to unexpected reaction kinetics for stream-borne solutes, compared to classical model predictions. We show that deeper, low-reactivity locations within the hyporheic zone retain solutes for extended periods, which delays reactions and causes solutes to persist at higher concentrations in the stream reach than would be predicted by classical approaches. These behaviors are captured by an upscaled model that reveals the fundamental physical and chemical processes in the hyporheic zone. We show how time scales …

Transcriptional Control Of Retinal Ganglion Cell Death After Axonal Injury., Stephanie B Syc-Mazurek, Hongtian Stanley Yang, Olivia J Marola, Gareth R Howell, Richard T Libby

Faculty Research 2022

Injury to the axons of retinal ganglion cells (RGCs) is a key pathological event in glaucomatous neurodegeneration. The transcription factors JUN (the target of the c-Jun N-terminal kinases, JNKs) and DDIT3/CHOP (a mediator of the endoplasmic reticulum stress response) have been shown to control the majority of proapoptotic signaling after mechanical axonal injury in RGCs and in other models of neurodegeneration. The downstream transcriptional networks controlled by JUN and DDIT3, which are critical for RGC death, however, are not well defined. To determine these networks, RNA was isolated from the retinas of wild-type mice and mice deficient in Jun, Ddit3, …

Adult Mouse Fibroblasts Retain Organ-Specific Transcriptomic Identity., Elvira Forte, Mirana Ramialison, Hieu T Nim, Madison Mara, Jacky Y Li, Rachel Cohn, Sandra Daigle, Sarah Boyd, Edouard G Stanley, Andrew G Elefanty, J Travis Hinson, Mauro W Costa, Nadia Rosenthal, Milena B Furtado

Faculty Research 2022

Organ fibroblasts are essential components of homeostatic and diseased tissues. They participate in sculpting the extracellular matrix, sensing the microenvironment, and communicating with other resident cells. Recent studies have revealed transcriptomic heterogeneity among fibroblasts within and between organs. To dissect the basis of interorgan heterogeneity, we compare the gene expression of murine fibroblasts from different tissues (tail, skin, lung, liver, heart, kidney, and gonads) and show that they display distinct positional and organ-specific transcriptome signatures that reflect their embryonic origins. We demonstrate that expression of genes typically attributed to the surrounding parenchyma by fibroblasts is established in embryonic development and …

The Apoeε3/Ε4 Genotype Drives Distinct Gene Signatures In The Cortex Of Young Mice, Kate E Foley, Amanda A Hewes, Dylan Garceau, Kevin P Kotredes, Gregory W. Carter, Michael Sasner, Gareth R Howell

Faculty Research 2022

Introduction: Restrictions on existing APOE mouse models have impacted research toward understanding the strongest genetic risk factor contributing to Alzheimer's disease (AD) and dementia, APOE^ε4 , by hindering observation of a key, common genotype in humans - APOE^ε3/ε4 . Human studies are typically underpowered to address APOE^ε4 allele risk as the APOE^ε4/ε4 genotype is rare, which leaves human and mouse research unsupported to evaluate the APOE^ε3/ε4 genotype on molecular and pathological risk for AD and dementia.

Methods: As a part of MODEL-AD, we created and validated new versions of humanized APOE^ε3/ε3 and APOE^{ε4/ε4 …}