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2022

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Toxicity And Repellency Of Plant Extract And Termiticide Against Fungus Growing Subterranean Termites (Blattodea: Termitidae), Muhammad Shahid Nisar, Talha Nazir, Shah Zaman, Syed Ismat Hussain, Nasir Ahmad Khan, Hafiz Muhammad Usman Aslam, Wajad Nazeer, Muhammad Akhtar Jun 2022

Toxicity And Repellency Of Plant Extract And Termiticide Against Fungus Growing Subterranean Termites (Blattodea: Termitidae), Muhammad Shahid Nisar, Talha Nazir, Shah Zaman, Syed Ismat Hussain, Nasir Ahmad Khan, Hafiz Muhammad Usman Aslam, Wajad Nazeer, Muhammad Akhtar

Journal of Bioresource Management

Different methods such as physical, biological and chemical are used to manage soil fungus increasing termites. Synthetic insecticide plays a vital part in the management of termites. The pesticide used in big quantities causes phytotoxicity, mammalian toxicity and resistance to pesticides in target pests and insect outbreaks. Intensive pesticides exert chronic effects on living organisms with annoyance for beneficial insects. It also accompanied with environmental hazards and developed resistance. Plant leaves extracts provide a distinct variety of biochemical compounds with diverse prospective uses. Resistance development requires the discovery of fresh biological compounds with a wide spectrum of action. Plant leaves …


Efficacy Of Insecticides Against Fall Armyworm, Spodoptera Frugiperda (Lepidoptera, Noctuidae) In Maize, Fawzan Masood Mian, Imtiaz Khan, Najeeb Ullah, Aqarab Husnain Gondal, Muhamad Saqib Ajmal, Muhammad Sajid Qureshi, Adnan Ihsan, Muhammad Raziq, Imran Qazi, Abdul Jabbar Jun 2022

Efficacy Of Insecticides Against Fall Armyworm, Spodoptera Frugiperda (Lepidoptera, Noctuidae) In Maize, Fawzan Masood Mian, Imtiaz Khan, Najeeb Ullah, Aqarab Husnain Gondal, Muhamad Saqib Ajmal, Muhammad Sajid Qureshi, Adnan Ihsan, Muhammad Raziq, Imran Qazi, Abdul Jabbar

Journal of Bioresource Management

Fall armyworm (FAW), Spodoptera frugiperda (Lepidoptera: Noctuidae) is most destructive specie of genus Spodoptera for several agricultural crops. In Pakistan's Sindh province, the invasive fall armyworm Spodoptera frugiperda was first documented causing serious maize damage in 2019. There is need to develop management strategies against this pest in the country. The current study was conducted to check the toxicity of different insecticides against FAW in maize field. The results showed among tested insecticides, deltamethrin was recorded most toxic insecticide followed by chlorantraniliprole and emamectin benzoate. At 1d days after first spray, least number of larvae were recorded with deltamethrin (0.07 …


Presence Of Blaper-1 And Blaveb-1 Beta-Lactamase Genes Among Isolates Of Pseudomonas Aeruginosa From Burn And Trauma Hospital Peshawar, Pakistan, Suleman Khan, Samiyah Tasleem, Alfarah Rehmat Ullah,, Sarwat Moon, Saad Alghamdi, Raina Saad Suliman, Muhammad Ateeq, Muhmmad Salman, Anas S. Dablool,, Banan Atwah, Farkad Bantun Jun 2022

Presence Of Blaper-1 And Blaveb-1 Beta-Lactamase Genes Among Isolates Of Pseudomonas Aeruginosa From Burn And Trauma Hospital Peshawar, Pakistan, Suleman Khan, Samiyah Tasleem, Alfarah Rehmat Ullah,, Sarwat Moon, Saad Alghamdi, Raina Saad Suliman, Muhammad Ateeq, Muhmmad Salman, Anas S. Dablool,, Banan Atwah, Farkad Bantun

Journal of Bioresource Management

Pseudomonas aeruginosa spp are the most prevalent bacteria that cause nosocomial infections in hospitals. Most antibiotics, including novel new β-lactams, are already resistant to them, and they can become resistant during treatment, which can make the treatment fail. P. aeruginosa isolates from ICU patients who had Per-1 and VEB-1 were the main focus of this study. These two ESBLs are the two most common in ICU patients who had them. 50 isolates were gathered from Peshawar's LRH ICU facilities in the year 2021. The antibiotic susceptibility test was conducted in accordance with the Clinical and Laboratory Standards Institute's standards (CLSI). …


Development Of Enhanced Food Packaging Systems Based On Blends Of Bio-Based And Biodegradable Polymers Exhibiting Antimicrobial Properties, Aishat Agboluaje Jun 2022

Development Of Enhanced Food Packaging Systems Based On Blends Of Bio-Based And Biodegradable Polymers Exhibiting Antimicrobial Properties, Aishat Agboluaje

Theses and Dissertations

Food packaging is one of the important aspects of food processing, which contributes greatly to decreasing post-harvest food losses. While ensuring that foods are adequately packaged, it is also important to consider the impact of these food packaging materials on the environment. Accordingly, this research work was aimed at investigating the potential of bio-based and biodegradable blends of polyvinyl alcohol (PVA) and chitosan loaded with antimicrobial agents such as turmeric, clove, and cinnamon for use in food packaging applications. The methodology involved creating PVA/chitosan films with varying compositions via solvent casting method and selecting the best composition exhibiting the highest …


Critical P, K And S Concentrations In Soil And Shoot Samples For Optimal Tedera Productivity And Nodulation, Daniel Real, Richard G. Bennett, Nazanin K. Nazeri, David M. Weaver Jun 2022

Critical P, K And S Concentrations In Soil And Shoot Samples For Optimal Tedera Productivity And Nodulation, Daniel Real, Richard G. Bennett, Nazanin K. Nazeri, David M. Weaver

Grain and Other Field Crops Research Articles

Tedera is a forage legume that can provide out-of-season green feed in Mediterranean climates. To date, growers have had no comprehensive soil nutrition guidelines to optimise tedera production. We undertook field and glasshouse studies to understand tedera’s macronutrient requirements. Three field experiments were sown with tedera cv. Lanza® at Cunderdin, Dandaragan and Three Springs in Western Australia. These experiments evaluated seven levels of phosphorus (P) (0–30 kg ha−1) and potassium (K) (0–80 kg ha−1) and two combined treatments with P and K. Glasshouse pot experiments were conducted using tedera cultivars Lanza® and Palma and lucerne cultivar SARDI Grazer. Ten concentrations …


Regulation Of Age-Associated Insulin Resistance By Mt1-Mmp-Mediated Cleavage Of Insulin Receptor, Sheung Kin Ken Wong, Lixiang Zhai, Zening Wang, Xin Ge, Zhixin Jiang, Jiayan Wu, Yijing Zhang, Xiaoyu Wu, Keyang Xu, Cheng Yuan Lin, Hiu Yee Kwan, Aiping Lyu, Zhongjun Zhou, Zhao-Xiang Bian, Hoi Leong Xavier Wong Jun 2022

Regulation Of Age-Associated Insulin Resistance By Mt1-Mmp-Mediated Cleavage Of Insulin Receptor, Sheung Kin Ken Wong, Lixiang Zhai, Zening Wang, Xin Ge, Zhixin Jiang, Jiayan Wu, Yijing Zhang, Xiaoyu Wu, Keyang Xu, Cheng Yuan Lin, Hiu Yee Kwan, Aiping Lyu, Zhongjun Zhou, Zhao-Xiang Bian, Hoi Leong Xavier Wong

Faculty, Staff and Student Publications

Insulin sensitivity progressively declines with age. Currently, the mechanism underlying age-associated insulin resistance remains unknown. Here, we identify membrane-bound matrix metalloproteinase 14 (MT1-MMP/MMP14) as a central regulator of insulin sensitivity during ageing. Ageing promotes MMP14 activation in insulin-sensitive tissues, which cleaves Insulin Receptor to suppress insulin signaling. MT1-MMP inhibition restores Insulin Receptor expression, improving insulin sensitivity in aged mice. The cleavage of Insulin Receptor by MT1-MMP also contributes to obesity-induced insulin resistance and inhibition of MT1-MMP activities normalizes metabolic dysfunctions in diabetic mouse models. Conversely, overexpression of MT1-MMP in the liver reduces the level of Insulin Receptor, impairing hepatic insulin …


Florida In Publications, 2016, Florida Historical Society Jun 2022

Florida In Publications, 2016, Florida Historical Society

Florida Historical Quarterly

No abstract provided.


Book Reviews, Florida Historical Society Jun 2022

Book Reviews, Florida Historical Society

Florida Historical Quarterly

de la Cova, Colonel Henry Theodore Titus: Antebellum Soldier of Fortune and Florida Pioneer. by Victor Andres Triay; Porcher Jr. and Judd, The Market Preparation of Carolina Rice: An lllustrated History of Innovations in the Lowcountry Rice Kingdom. by Hayden R. Smith; Warren, The Rebel Yell: A Cultural History. by Michael T. Bernath; Dom, Challenges on the Emmaus Road: Episcopal Bishops Confront Slavery, Civil War, and Emancipation. by Glenn Robins; McKinley, Stinking Stones and Rocks of Gold: Phosphate, Fertilizer, and Industrialization in Postbellum South Carolina. by Peter A. Coclanis; Stanonis, Faith in Bikinis: Politics and Leisure in the Coastal South …


Book Reviews, Florida Historical Society Jun 2022

Book Reviews, Florida Historical Society

Florida Historical Quarterly

Zettler, The Biohistory of Florida, by James C. Clark; Osborn, Indian River Lagoon: An Environmental History. by Devin Leigh; Emberton, Beyond Redemption: Race, Violence, and the American South after the Civil War. by Gregory Mixon; Escott, Uncommonly Savage: Civil War and Remembrance in Spain and the United States. by James W. Cortada; Downs, Declarations of Dependence: The Long Reconstruction of Popular Politics in the South, 1861-1908. by Margaret M. Mulrooney; Bennett, When Tobacco Was King: Families, Farm Labor, and Federal Policy in the Piedmont. by Peter Benson; Vickers and Wilson-Graham, Remembering Paradise Park: Tourism and Segregation at Silver Springs. by …


Post-Exercise Arterial Stiffness Responses Are Similar After Acute Eccentric And Concentric Arm Cycling, Travis R. Wakeham, Dakota J. Anderson, Steven J. Elmer, John J. Durocher Jun 2022

Post-Exercise Arterial Stiffness Responses Are Similar After Acute Eccentric And Concentric Arm Cycling, Travis R. Wakeham, Dakota J. Anderson, Steven J. Elmer, John J. Durocher

International Journal of Exercise Science

International Journal of Exercise Science 15(2): 884-895, 2022. Upper-body resistance exercise effectively increases muscular strength, but may concomitantly increase arterial stiffness. Eccentric exercise can lead to muscle soreness and arterial stiffness in untrained participants. However, it is unclear if upper-body eccentric exercise could reduce arterial stiffness in a single session for participants that have undergone progressive training. Our purpose was to compare acute responses to upper-body eccentric (novel, ECCarm) and concentric (traditional, CONarm) steady state arm cycling. We hypothesized that arm arterial stiffness would be reduced after both ECCarm and CONarm. Twenty-two young healthy individuals performed either ECCarm (n …


Food Allergies And Disabilities In Children, Angela D. Vaughn Jun 2022

Food Allergies And Disabilities In Children, Angela D. Vaughn

LC Journal of Special Education

There is increased curiosity and research regarding the role that food allergies may or may not have in relation to major disabilities in children. Some researchers claim that symptoms, from simple aches and pains to cancer, are caused by food allergies. More specifically, in regards to children, there has been much speculation and research concerning specific food allergies and its possible link to two major disabilities in particular, Attention Deficit Hyperactivity Disorder (ADHD) and autism.


Investigating The Overrepresentation Of Ethnic Minorities In Special Education, Damien Murtagh Jun 2022

Investigating The Overrepresentation Of Ethnic Minorities In Special Education, Damien Murtagh

LC Journal of Special Education

Debates on the overrepresentation of minority students, particularly African- Americans, are not new in special education and have characterized research in this field for over three decades. Regardless of time, legislative debate and a host of research theories, this problem remains. “In general, research has supported the public concern but the picture is unclear because studies have varied so much with respect to definitions of minority representation in findings across ethnic groups and technical methods (Coutinho & Oswald, 1999, p. 66). It is on the definition of minorities, the methods used to calculate date and the identification of minority students …


Reactive Attachment Disorder: A Review, Malia C. King Jun 2022

Reactive Attachment Disorder: A Review, Malia C. King

LC Journal of Special Education

Using recent research, this paper will provide an overview of reactive attachment disorder, its symptoms, characteristics and causal factors, as well as assessment and treatment tools. It will conclude with a discussion of controversies relating to the present DSM-IV definition as well as attention to prevention considerations.


Williams Syndrome, Vickie Davis Jun 2022

Williams Syndrome, Vickie Davis

LC Journal of Special Education

Williams syndrome is known by several names: Beuren's syndrome, Williams-Beuren syndrome, Fanconi-Schlesinger syndrome, Williams‟ elfin face syndrome, Williams-Barratt syndrome, and Williams‟ syndrome (Also known as . . . 2001). Williams syndrome (WS) affects 1 in 7500-20,000 births regardless of gender or race (Stromme, Bjornstad, and Ramstad 2002). It is typically diagnosed in infancy or in early childhood. Recently, there has been a great deal of research regarding all aspects of this perplexing syndrome. Therefore, the scope of this paper will be an overview of the history, etiology, characteristics, and interventions surrounding Williams syndrome.


Contribución A La Conservación De La Orquídea Rodriguezia Granadensis (Lindl.) Rchb.F. En La Hacienda Betania (Fusagasugá, Colombia), Arlette Ivonne Gil Clavijo, Jenny Paola Moreno Lopez, Laguandio Del Cristo Banda Sánchez Jun 2022

Contribución A La Conservación De La Orquídea Rodriguezia Granadensis (Lindl.) Rchb.F. En La Hacienda Betania (Fusagasugá, Colombia), Arlette Ivonne Gil Clavijo, Jenny Paola Moreno Lopez, Laguandio Del Cristo Banda Sánchez

Ciencias Agropecuarias

Esta cartilla, creada por el Grupo de Investigación PROSAFIS de la Universidad de Cundinamarca, presenta los resultados del proyecto Aproximación a la ecología de orquídeas nativas en el ecosistema hacienda Betania con proyección a su conservación, manejo y producción. Se destaca la orquídea Rodriguezia granadensis, adaptada a forofitos (phorophytes) de cítricos y guayabos, representando el 83,3 % de los individuos observados. Predominó la fase vegetativa (fenología) durante todo el período, con más floración y cápsulas en épocas lluviosas, sincronizando su fase reproductiva con el régimen de lluvias. En las raíces se encontró el hongo endófito (endophyte) Rhizoctonia en …


Cell Origin-Dependent Cooperativity Of Mutant Dnmt3a And Npm1 In Clonal Hematopoiesis And Myeloid Malignancy., Jennifer M Sanmiguel, Elizabeth Eudy, Matthew A Loberg, Linde A Miles, Timothy M Stearns, Jayna J Mistry, Michael J Rauh, Ross L Levine, Jennifer J. Trowbridge Jun 2022

Cell Origin-Dependent Cooperativity Of Mutant Dnmt3a And Npm1 In Clonal Hematopoiesis And Myeloid Malignancy., Jennifer M Sanmiguel, Elizabeth Eudy, Matthew A Loberg, Linde A Miles, Timothy M Stearns, Jayna J Mistry, Michael J Rauh, Ross L Levine, Jennifer J. Trowbridge

Faculty Research 2022

In adult acute myeloid leukemia (AML), the acquisition of driver somatic mutations may be preceded by a benign state termed clonal hematopoiesis (CH). To develop therapeutic strategies to prevent leukemia development from CH, it is important to understand the mechanisms by which CH-driving and AML-driving mutations cooperate. Here, we use mice with inducible mutant alleles common in human CH (DNMT3AR882; mouse Dnmt3aR878H) and AML (NPM1c; mouse Npm1cA). We find that Dnmt3aR878H/+ hematopoietic stem cells (HSCs), but not multipotent progenitor cell (MPP) subsets, have reduced cytokine expression and proinflammatory transcriptional signatures and a functional competitive advantage over their wild-type counterparts. Dnmt3aR878H/+ …


Transcriptional And Translational Dynamics Of Zika And Dengue Virus Infection., Kamini Singh, Maria Guadalupe Martinez, Jianan Lin, James Gregory, Trang Uyen Nguyen, Rawan Abdelaal, Kristy Kang, Kristen Brennand, Arnold Grünweller, Zhengqing Ouyang, Hemali Phatnani, Margaret Kielian, Hans-Guido Wendel Jun 2022

Transcriptional And Translational Dynamics Of Zika And Dengue Virus Infection., Kamini Singh, Maria Guadalupe Martinez, Jianan Lin, James Gregory, Trang Uyen Nguyen, Rawan Abdelaal, Kristy Kang, Kristen Brennand, Arnold Grünweller, Zhengqing Ouyang, Hemali Phatnani, Margaret Kielian, Hans-Guido Wendel

Faculty Research 2022

Zika virus (ZIKV) and dengue virus (DENV) are members of the Flaviviridae family of RNA viruses and cause severe disease in humans. ZIKV and DENV share over 90% of their genome sequences, however, the clinical features of Zika and dengue infections are very different reflecting tropism and cellular effects. Here, we used simultaneous RNA sequencing and ribosome footprinting to define the transcriptional and translational dynamics of ZIKV and DENV infection in human neuronal progenitor cells (hNPCs). The gene expression data showed induction of aminoacyl tRNA synthetases (ARS) and the translation activating PIM1 kinase, indicating an increase in RNA translation capacity. …


Identity Formation Of Migrant Characters In Catalin Dorian Florescu’S Novels Jacob Beschließt Zu Lieben And Der Mann, Der Das Glück Bringt, Aratee Kaewsumrit Jun 2022

Identity Formation Of Migrant Characters In Catalin Dorian Florescu’S Novels Jacob Beschließt Zu Lieben And Der Mann, Der Das Glück Bringt, Aratee Kaewsumrit

Journal of Letters

This research article outlines the process of identity formation of migrant characters in the novels Jacob beschließt zu lieben (2011) and Der Mann, der das Glück bringt (2016) by Catalin Dorian Florescu, a famous contemporary Swiss author who was born in Romania. The study shows that the characters in both novels form their identities in similar ways. Due to hard social contexts and their longings for survival, they form flexible and fluid identities which do not adhere to their ethnic and cultural roots. Such identity formation which crosses cultural boundaries, and the settings in places of cultural hybridity like Banat …


Methods And Devices For Sample Capture Using Gas-Pulse Nanoparticle Displacement, Kermit King Murray, Fabrizio Donnarumma, Jamira Stephenson Jun 2022

Methods And Devices For Sample Capture Using Gas-Pulse Nanoparticle Displacement, Kermit King Murray, Fabrizio Donnarumma, Jamira Stephenson

College of Science Patents

The present disclosure provides for sampling instruments and methods of collecting sample particles. The sampling instrument can include a high-pressure pulsed valve coupled to a gas flow system to displace a sample from a surface. Also included can be a voltage supply coupled to a voltage switch, a suction device, a sample collector, and a collection filter. To collect a sample, extractive particles can be deposited onto a sample present on a substrate. At least a portion of the sample becomes coupled to a portion of the extractive particles to form sample particles. High-pressure gas can be discharged at the …


Ptrwox13a Promotes Wood Formation And Bioactive Gibberellins Biosynthesis In Populus Trichocarpa, Yang Zhang, Yingying Liu, Xueying Wang, Ruiqi Wang, Xuebing Chen, Shuang Wang, Hairong Wei, Zhigang Wei Jun 2022

Ptrwox13a Promotes Wood Formation And Bioactive Gibberellins Biosynthesis In Populus Trichocarpa, Yang Zhang, Yingying Liu, Xueying Wang, Ruiqi Wang, Xuebing Chen, Shuang Wang, Hairong Wei, Zhigang Wei

Michigan Tech Publications, Part 1

WUSCHEL-related homeobox (WOX) genes are plant-specific transcription factors (TFs) involved in multiple processes of plant development. However, there have hitherto no studies on the WOX TFs involved in secondary cell wall (SCW) formation been reported. In this study, we identified a Populus trichocarpa WOX gene, PtrWOX13A, which was predominantly expressed in SCW, and then characterized its functions through generating PtrWOX13A overexpression poplar transgenic lines; these lines exhibited not only significantly enhanced growth potential, but also remarkably increased SCW thicknesses, fiber lengths, and lignin and hemicellulose contents. However, no obvious change in cellulose content was observed. We revealed that PtrWOX13A directly …


Recognition Of The Tdp-43 Nuclear Localization Signal By Importin Α1/Β, Steven G Doll, Hamed Meshkin, Alexander J Bryer, Fenglin Li, Ying-Hui Ko, Ravi K Lokareddy, Richard E Gillilan, Kushol Gupta, Juan R Perilla, Gino Cingolani Jun 2022

Recognition Of The Tdp-43 Nuclear Localization Signal By Importin Α1/Β, Steven G Doll, Hamed Meshkin, Alexander J Bryer, Fenglin Li, Ying-Hui Ko, Ravi K Lokareddy, Richard E Gillilan, Kushol Gupta, Juan R Perilla, Gino Cingolani

Department of Biochemistry and Molecular Biology Faculty Papers

Cytoplasmic mislocalization of the TAR-DNA binding protein of 43 kDa (TDP-43) leads to large, insoluble aggregates that are a hallmark of amyotrophic lateral sclerosis and frontotemporal dementia. Here, we study how importin α1/β recognizes TDP-43 bipartite nuclear localization signal (NLS). We find that the NLS makes extensive contacts with importin α1, especially at the minor NLS-binding site. NLS binding results in steric clashes with the C terminus of importin α1 that disrupts the TDP-43 N-terminal domain (NTD) dimerization interface. A putative phosphorylation site in the proximity of TDP-43 R83 at the minor NLS site destabilizes binding to importins by reducing …


Explainable And Cooperative Autonomy Across Networks Of Distributed Systems, Peter Joseph Jorgensen Jun 2022

Explainable And Cooperative Autonomy Across Networks Of Distributed Systems, Peter Joseph Jorgensen

USF Tampa Graduate Theses and Dissertations

Large networks of complex systems-of-systems are commonplace and evermore present in both mundane and extraordinary facets of human existence. From the exponential growth of connectivity via the internet and other information networks, to the miniaturization of computers and sensors, to cross-domain sensor and communication networks, these networks of distributed systems-of-systems (NDSS) present incredible benefits and challenges. Autonomy is perhaps the most important and most difficult to achieve enabling technology for efficient performance of the NDSS. Giving each individual agent in a network the ability to manage its internal state in dynamic operating environments and in pursuit of multiple complex and …


Nf-Kb (P50/P65)-Mediated Pro-Inflammatory Microrna (Mirna) Signaling In Alzheimer's Disease (Ad), Walter J. Lukiw Jun 2022

Nf-Kb (P50/P65)-Mediated Pro-Inflammatory Microrna (Mirna) Signaling In Alzheimer's Disease (Ad), Walter J. Lukiw

School of Medicine Faculty Publications

No abstract provided.


Nanophotonics And Nanomaterials For Microbial Inactivation, Sharad Ambardar Jun 2022

Nanophotonics And Nanomaterials For Microbial Inactivation, Sharad Ambardar

USF Tampa Graduate Theses and Dissertations

The study of light-matter interaction at nanoscale, also termed as nanophotonics, has gained vast attention due to its multidisciplinary application in the field of chemical engineering for the synthesis of nanomaterials, in the field of physics to study non-linear optical processes and optical phenomena in nanocavities and in the field of biology, biomedicine to study and develop novel optical nanoprobes for diagnostics, nanobiosensing and near-field imaging.

We studied UV-irradiation-based inactivation of SARS-CoV-2 and other respiratory viruses. In this work, we fabricated a device comprising a pulsed nanosecond 266 nm UV laser coupled to an integrating cavity (LIC), composed of a …


Hope 4 Boys: The Impact Of A Youth Diversion Program, Justine C. Connolly Jun 2022

Hope 4 Boys: The Impact Of A Youth Diversion Program, Justine C. Connolly

USF Tampa Graduate Theses and Dissertations

The Florida Department of Juvenile Justice (DJJ) is developing programs targeting first-time nonviolent offenders who might benefit from community-based treatment and positive youth development to prevent these individuals from becoming adult criminals. Thus, the DJJ seeks new and innovative ways to prevent juvenile delinquency and reduce recidivism among first-time nonviolent offenders. The present study aimed to evaluate the Hope 4 Boys program, a juvenile diversion program that addresses the academic and social-emotional needs of juvenile delinquents. The study examined how participation in the program is associated with changes in participants’ levels of hope for the future, life satisfaction, and resiliency …


Isolation Of A Potently Neutralizing And Protective Human Monoclonal Antibody Targeting Yellow Fever Virus, Michael P Doyle, Joseph R Genualdi, Adam L Bailey, Nurgun Kose, Christopher Gainza, Jessica Rodriguez, Kristen M Reeder, Christopher A Nelson, Prashant N Jethva, Rachel E Sutton, Robin G Bombardi, Michael L Gross, Justin G Julander, Daved H Fremont, Michael S Diamond, James E Crowe Jun 2022

Isolation Of A Potently Neutralizing And Protective Human Monoclonal Antibody Targeting Yellow Fever Virus, Michael P Doyle, Joseph R Genualdi, Adam L Bailey, Nurgun Kose, Christopher Gainza, Jessica Rodriguez, Kristen M Reeder, Christopher A Nelson, Prashant N Jethva, Rachel E Sutton, Robin G Bombardi, Michael L Gross, Justin G Julander, Daved H Fremont, Michael S Diamond, James E Crowe

Open Access Publications

Yellow fever virus (YFV) causes sporadic outbreaks of infection in South America and sub-Saharan Africa. While live-attenuated yellow fever virus vaccines based on three substrains of 17D are considered some of the most effective vaccines in use, problems with production and distribution have created large populations of unvaccinated, vulnerable individuals in areas of endemicity. To date, specific antiviral therapeutics have not been licensed for human use against YFV or any other related flavivirus. Recent advances in monoclonal antibody (mAb) technology have allowed the identification of numerous candidate therapeutics targeting highly pathogenic viruses, including many flaviviruses. Here, we sought to identify …


Structure Of A Vaccine-Induced, Germline-Encoded Human Antibody Defines A Neutralizing Epitope On The Sars-Cov-2 Spike N-Terminal Domain, Clara G Altomare, Daniel C Adelsberg, Juan Manuel Carreno, Iden A Sapse, Fatima Amanat, Ali H Ellebedy, Viviana Simon, Florian Krammer, Goran Bajic Jun 2022

Structure Of A Vaccine-Induced, Germline-Encoded Human Antibody Defines A Neutralizing Epitope On The Sars-Cov-2 Spike N-Terminal Domain, Clara G Altomare, Daniel C Adelsberg, Juan Manuel Carreno, Iden A Sapse, Fatima Amanat, Ali H Ellebedy, Viviana Simon, Florian Krammer, Goran Bajic

Open Access Publications

Structural characterization of infection- and vaccination-elicited antibodies in complex with antigen provides insight into the evolutionary arms race between the host and the pathogen and informs rational vaccine immunogen design. We isolated a germ line-encoded monoclonal antibody (mAb) from plasmablasts activated upon mRNA vaccination against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and determined its structure in complex with the spike glycoprotein by electron cryomicroscopy (cryo-EM). We show that the mAb engages a previously uncharacterized neutralizing epitope on the spike N-terminal domain (NTD). The high-resolution structure reveals details of the intermolecular interactions and shows that the mAb inserts its heavy …


Invl, An Invasin-Like Adhesin, Is A Type Ii Secretion System Substrate Required For Acinetobacter Baumannii Uropathogenesis, Clay D Jackson-Litteken, Gisela Di Venanzio, Nguyen-Hung Le, Nichollas E Scott, Bardya Djahanschiri, Jesus S Distel, Evan J Pardue, Ingo Ebersberger, Mario F Feldman Jun 2022

Invl, An Invasin-Like Adhesin, Is A Type Ii Secretion System Substrate Required For Acinetobacter Baumannii Uropathogenesis, Clay D Jackson-Litteken, Gisela Di Venanzio, Nguyen-Hung Le, Nichollas E Scott, Bardya Djahanschiri, Jesus S Distel, Evan J Pardue, Ingo Ebersberger, Mario F Feldman

Open Access Publications

Acinetobacter baumannii is an opportunistic pathogen of growing concern, as isolates are commonly multidrug resistant. While A. baumannii is most frequently associated with pulmonary infections, a significant proportion of clinical isolates come from urinary sources, highlighting its uropathogenic potential. The type II secretion system (T2SS) of commonly used model Acinetobacter strains is important for virulence in various animal models, but the potential role of the T2SS in urinary tract infection (UTI) remains unknown. Here, we used a catheter-associated UTI (CAUTI) model to demonstrate that a modern urinary isolate, UPAB1, requires the T2SS for full virulence. A proteomic screen to identify …


Ultrasmall Nanodiamonds: Perspectives And Questions, Shery L.Y. Chang, Philipp Reineck, Anke Krueger, Vadym Mochalin Jun 2022

Ultrasmall Nanodiamonds: Perspectives And Questions, Shery L.Y. Chang, Philipp Reineck, Anke Krueger, Vadym Mochalin

Chemistry Faculty Research & Creative Works

Nanodiamonds are at the heart of a plethora of emerging applications in areas ranging from nanocomposites and tribology to nanomedicine and quantum sensing. The development of alternative synthesis methods, a better understanding, and the availability of ultrasmall nanodiamonds of less than 3 nm size with a precisely engineered composition, including the particle surface and atomic defects in the diamond crystal lattice, would mark a leap forward for many existing and future applications. Yet today, we are unable to accurately control nanodiamond composition at the atomic scale, nor can we reliably create and isolate particles in this size range. In this …


Deutetrabenazine For The Treatment Of Chorea Associated With Huntington’S Disease, Harshit Gupta, Wil Perkins, Cain Stark, Sathya Kikkeri, Juyeon Kakazu, Adam M. Kaye, Alan David Kaye Jun 2022

Deutetrabenazine For The Treatment Of Chorea Associated With Huntington’S Disease, Harshit Gupta, Wil Perkins, Cain Stark, Sathya Kikkeri, Juyeon Kakazu, Adam M. Kaye, Alan David Kaye

School of Pharmacy Faculty Articles

This is a comprehensive review of the literature regarding the use of Deutetrabenazine in treating chorea associated with Huntington’s disease. Unfortunately, treatment has been limited for many aspects of this neurodegenerative disease. The present investigation presents the background, evidence, and indications for the use Deutetrabenazine in the setting of Huntington’s disease. Huntington’s disease is characterized by a variety of motor, psychiatric, and cognitive symptoms with chorea being one of the more notable ones. Chorea is a movement disorder present in multiple neurologic diseases that causes involuntary and irregular muscle movements theorized to be stemming from high dopamine levels. Deutetrabenazine is …