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Bacterial Community Dynamics In Rhode Island Coastal Ecosystems, Zachary Pimentel

Open Access Dissertations

In temperate coastal ecosystems, there are a variety of potential environmental niches and lifestyles for bacteria to occupy, along with constantly changing environmental conditions. Residing in such a diverse environment with broad habitats, bacterial adaptation can be observed at both the community and genome level. For example, an individual strain may acquire new mutations or genes to respond to varying conditions or occupy a new niche. In addition, the taxonomic composition of bacterial communities can shift, resulting in higher abundances of strains specialized for a new condition. In this dissertation, community and genome-level adaptations to varying lifestyles and environmental change …

Changes To California Alfalfa Production And Perceptions During The 2011-2017 Drought, Alida A. Cantor, Bethani Turley, Charles Cody Ross, Mathern Glass

Geography Faculty Publications and Presentations

California experienced a severe multiyear drought stretching from 2011 through 2017, significantly reducing surface water supply for ecosystems, agriculture, and humans and prompting coordinated conservation efforts. Given that agriculture is the largest consumptive use of water in the state, one anticipated response to a severe drought would be to decrease production of low-value, high-water-use crops such as alfalfa. In this article we use a multimethod approach to examine both spatial distribution and public perceptions of alfalfa production in California over the course of the 2011 through 2017 drought. We find that although California alfalfa production did decline at the state …

Self-Reported And Behavioral Perseverance Associated With The Maintenance Of Health Behaviors, Alivia Kay Frazier

Graduate Theses, Dissertations, and Problem Reports (ETD)

Research has shown that positive psychological traits, such as perseverance, may be associated with the maintenance of health behaviors aimed to prevent commonly fatal diseases (e.g., cardiovascular disease, stroke). Although there is limited research in this area, the current study examined whether there was an association between self-reported perseverance, behavioral perseverance, and distress tolerance with various health behaviors (e.g., sleep, physical activity, nutrition, vaping, alcohol use, general health status, and COVID-19 behaviors). A novel methodology was implemented for this study that employed using the online mirror tracing task as a measure of behavioral perseverance.

A sample of young adult participants …

Barriers And Opportunities To Exiting Prostitution: An Analysis Of Prostitution Diversion Programs, Marina Binti Mohd Hamdan

Graduate Theses, Dissertations, and Problem Reports (ETD)

Problem-solving courts incorporate therapeutic and punitive measures in the court process in the criminal justice system. Prostitution Diversion Programs (PDP) are recognition of women in prostitution's inherent systemic struggles to desist and recover. Specialized Prostitution Diversion in Baltimore City and Project Dawn Court in Philadelphia are two PDPs analyzed through a longitudinal study on participants throughout their involvement as well as after they graduate from the program. By addressing the barriers to exiting prostitution through women’s turning points and providing opportunities to overcome hardships, women are more likely to successfully exit prostitution and maintain their sobriety in the long-term. Findings …

Adherence To Dietary Recommendations For Adolescents With Cystic Fibrosis, Kristine M. Durkin

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Adhering to dietary recommendations and nutritional supplements to ensure adequate absorption of nutrients and malnutrition plays an important role in the health of patients with cystic fibrosis (CF). Non-adherence to the CF care regimen, including nutritional recommendations, is high in adolescents. Therefore, the overall goal of this study was to identify factors related to adherence to nutritional recommendations in adolescents with CF.

Method: A total of 42 adolescents (ages 12-18) were recruited from 5 CF centers across the US. Adolescents completed surveys measuring body image/satisfaction, health literacy, perceived barriers to enzyme use, and self-efficacy, as well as the quality …

Effects Of Depression And Stress On Oral Self-Care Among Perinatal Women In Appalachia: A Longitudinal Study, Jeongwi An

Graduate Theses, Dissertations, and Problem Reports (ETD)

Oral health during the perinatal period has an impact on the health of women and their offspring. Oral self-care behaviors are preventive methods that can be employed to maintain or improve oral health but may be compromised during the perinatal period. Identifying factors affecting oral self-care behaviors might provide direction on how to intervene in oral health during and after pregnancy. The purpose of this study was to investigate independent and interactive effects of depression and stress on oral self-care behaviors of perinatal women in Appalachia, given the burden of oral disease in this region. A total of 1,172 women …

Feed Conditioning Effects On Enzymes, Amino Acids, And Subsequent Broiler Performance, Elizabeth Ann Lynch

Graduate Theses, Dissertations, and Problem Reports (ETD)

There are many things that can be done to improve broiler performance including, but not limited to, pelleting, proper Lys inclusion, and exogenous enzyme supplementation. Pelleting has the ability to improve feed form, reduce wastage, improve bird performance metrics, and decrease pathogens within the feed. Pathogen reduction can be further reduced by hygienic pelleting practices which include an increase in temperature or retention time within a conditioner or hygieniser. However, hygienic pelleting can degrade valuable AA and enzymes (more specifically for the current thesis research Lys and phytase) and make them unavailable to the animal. Supplementing Lys to account for …

The Effects Of Feed Additives And The Use Of A Novel Wood Boiler Heat Exchanger On Litter Quality, Broiler Performance, And Immune Status, Victoria Ayres

Graduate Theses, Dissertations, and Problem Reports (ETD)

Experiments were conducted to investigate the effects of varying feed ingredients and heating systems on boiler performance metrics. Chapter Two investigated the effects of two corn-expressed phytases, differing in enzyme concentration within the grain (3,000 or 9,677 FTU/g), on broiler performance and tibia mineralization, when used as a component in pelleted feed. Dietary treatments included a positive control (PC), with industry-recommended levels of calcium and phosphorous, and a negative control (NC), lower in calcium and non-phytate phosphorous. Six diets containing corn-expressed phytase at high or low grain concentrations, formulated to 3,000, 6,000, or 9,000 FTU/kg, were …

Investigation Of A Novel Throughput Agent On Production Rate And Pellet Quality And The Development Of A Lab-Based Method To Predict Salmonella Reduction In Poultry Feed, Utilizing Varying Feed Manufacture Parameters, Timothy Paul Boltz

Graduate Theses, Dissertations, and Problem Reports (ETD)

Two experiments were conducted to evaluate a novel throughput agent and its mechanisms on production rate and pellet quality. An additional experiment using lab-based methods and models was conducted to predict Salmonella reduction in mash broiler feed. Feed mill pellet production rate is of great importance to integrated meat bird production. Feed hygienics are also of increasing concern for poultry producers.

In chapter 2, the effect of AZOMITE® (AZM) and varying inorganic phosphate sources (IPS) on pellet mill production rate and pellet quality was assessed. It is documented that the naturally abrasive properties of inorganic phosphate sources may scour the …

Characterization Of Two Krab-Containing Zinc Finger Transcription Factors In Bovine Preimplantation Embryonic Development, Mingxiang Zhang

Graduate Theses, Dissertations, and Problem Reports (ETD)

Oocyte developmental competence or oocyte intrinsic quality describes the capability of oocytes to resume meiosis, cleave and develop to blastocyst stage after fertilization, implant and develop to term in a good health. A growing number of evidences indicate that the majority of embryonic mortality occurs during early embryonic development in different species, including human, horse and cattle primarily due to poor oocyte quality. Maternal effect genes are key aspects of oocyte quality, which are transcribed during the process of oogenesis and folliculogenesis. The maternal factors are accumulated in oocytes, orchestrating various early developmental events including fertilization, epigenetic reprogramming and zygotic …

The Heart Of Everything In The Middle Of Nowhere: The Role Of Rural Identity In The Formation And Deployment Of Political Attitudes In Pennsylvania, Mikaela G. Zimmerman

Graduate Theses, Dissertations, and Problem Reports (ETD)

The world of American politics continues to infiltrate households across the United States as technological advancement extends the reach of breaking news and government action. With this expanding reach, communities all over the country are digesting and contemplating their place in national politics more fervently than ever. At the crux of this discussion is the backbone of political engagement and action—identity and its resulting political attitudes. For decades, partisanship has been a point of contention amongst American citizens. Cities across the nation showcase protests, demonstrations, town hall meetings, and more illustrating citizens’ care for their democratized input in government affairs. …

Critical Perspectives On Produce Prescription Programs & Us Federal Nutrition Policy, Alanna K. Higgins

Graduate Theses, Dissertations, and Problem Reports (ETD)

Produce prescription programs, interventions operating at the intersection of food access and public health, are steadily increasing in number across the United States since 2010. I leverage key informant interviews, participant observations, and event ethnographies to form a four-year institutional ethnography of the implementation of produce prescriptions within West Virginia alongside a legal-policy archaeology methodology to understand how produce prescriptions have been institutionalized and funded within the US Farm Bill. While much of produce prescription program growth is attributed to an expansion of federal funding starting in 2014, this dissertation demonstrates that these programs and the federal legislation which has …

Sleep And Cardiovascular Reactivity To An Acute Virtual Stressor, Alaina Grace Tiani

Graduate Theses, Dissertations, and Problem Reports (ETD)

Inappropriate response and magnitude of cardiovascular reactivity (CVR) to stress is a proposed mechanism through which environmental stressors are linked with poor cardiovascular health outcomes (Chida & Steptoe, 2010; Krantz & Manuck, 1984). Studies of reactivity to various laboratory tasks commonly control for factors known to influence CVR such as smoking, medication use, caffeine intake, and BMI; however, few have considered the influence of sleep on CVR. In order to determine whether sleep characteristics need to be assessed and considered in studies of CVR, this study aimed to examine the association between indices of sleep quality and the magnitude and …

Relation Of Child Feeding Behaviors & Caregiver Qualities To Adherence To Nutritional Therapy In Type 1 Diabetes Management, Corrine N. Ahrabi-Nejad

Graduate Theses, Dissertations, and Problem Reports (ETD)

Type 1 diabetes (T1D) is a chronic illness, typically diagnosed within childhood, that affects the ability of the pancreas to produce insulin. Significant daily self-management behaviors are required to maintain metabolic control for patients with T1D. Nutritional therapy, including adequate intake of macronutrients, is one self-management behavior necessary to optimize health. Adherence to such nutritional guidelines is associated with improved glycemic control, decreased instances of acute health complications (e.g., hyperglycemia, hypoglycemia), and can also prevent insulin resistance. Despite the critical importance of nutritional therapy, adherence to these guidelines remains low within pediatric populations. Therefore, it is important and necessary to …

Playing-Related Medical Injuries And Health Conditions In Collegiate Saxophonists: A Survey Of Saxophonists In North American Universities, Michael Anne Tolan

Graduate Theses, Dissertations, and Problem Reports (ETD)

When compared to other areas of musical performance, medical research on the performing arts is limited when it comes to studies on woodwind players, particularly saxophonists. The gap in research may be attributed to the circumstances surrounding selection processes and identifying subjects for study. For example, saxophonists are not salaried professionals in orchestras, which are commonly pooled for medical research. Establishing contact with saxophonists to conduct studies can prove difficult due to the wide range of genres that utilize the saxophone. Some issues may also be attributed to research methodology. To find the onset of injuries, researchers in previous studies …

What Is The Perceived Self-Efficacy Of Defined Job Competencies For West Virginia University Extension Faculty?, Lacey Dawn Seckman

Graduate Theses, Dissertations, and Problem Reports (ETD)

The central mission of the Extension Service is to diffuse knowledge and resources developed by teaching and research experts at land grant universities to clientele within local communities (Cummings, et al., 2015; Hall & Broyles, 2016; Seevers & Graham, 2012). Making sure faculty are prepared to carry out their roles in all aspects to serve their clientele is key to quality Extension programs. Extension faculty can come from various backgrounds, interests, and expertise, all of which are influencers of self-efficacy. Having defined competencies for employees in Extension can help express what to expect in this job day-to-day. McClelland (1973) described …

The Process And Case Study Approach To Implementing A Nutrition-Specific Dissemination And Implementation Science Training In Appalachia, Ayron E. Walker

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction: Nutrition professionals face implementation challenges due to their lack of knowledge to incorporate D&I science into intervention design. To improve intervention outcomes, nutrition professionals must be trained in D&I science, which are currently absent in the field.

Aims: To identify and assess nutrition educators and practitioners perceived motivations, self-efficacy, and attitudes in using D&I science to inform the educational design and strategies of a nutrition-specific D&I curriculum for nutrition educators. Then to test feasibility of the nutrition-specific curriculum in increasing knowledge, self-efficacy, and attitudes by a case study approach, pre/post intervention design.

Methods: First, scoping review methodology involved the …

Identification Of Sesquiterpenes In Wild Tomato Accessions With Activities Against The Potato Aphid And Their Tissue-Specific Engineering In Cultivated Tomato, Fumin Wang

Graduate Theses, Dissertations, and Problem Reports (ETD)

The potato aphid (Macrosiphum euphorbiae Thomas) poses a serious problem in the commercial production of horticultural crops including tomato, since it causes damage by stylet feeding and the transmission of viruses for which it serves as the vector. Application of conventional pesticides being the fundamental tactic in the control of the aphid is increasingly considered insufficient and problematic due to emerging pest resistances and biosafety issues, highlighting the continuing need to develop new efficient and sustainable approaches. Alternatively, glandular trichomes of plants are well-known epidermal hairy tissues producing various secondary metabolites that involve in plant-insect interactions, while the terpene …

Targeting Vulnerabilities In Cell State And Calcium Signaling For The Treatment Of Lung Cancer, Clark Jones

Graduate Theses, Dissertations, and Problem Reports (ETD)

Lung cancer remains the deadliest of all cancers due to the high mutational burden associated with the disease. Combating mutational drivers and drug resistance proves to be essential in the development of novel therapies to improve patient outcomes. A first-in-class cyclic peptide known as MTI-101 has been shown to induce necrotic cell death in a caspase independent manner. MTI-101 was derived from the linear peptide known as HYD1 that was found in a high throughput screen to block cell adhesion with the extracellular matrix. The compound was further optimized and cyclized to the currently used MTI-101 that was found to …

Impact Of Haemonchus Contortus On Feed Efficiency And Feeding Behavior Of Katahdin And Suffolk Lambs, Camren L. Maierle

Graduate Theses, Dissertations, and Problem Reports (ETD)

Nutritional impact of internal parasitism, specifically Haemonchus contortus (Hc), is a significant contributor to production losses in small ruminants. Both chronic and subclinical infections generate millions of dollars of losses worldwide. Understanding the nutritional impact of Hc on lamb performance is crucial in determining the additional nutrient requirements of infected individuals. The aim of this study was to evaluate nutritional impact of Hc on infected Katahdin lambs with extensive genetic selection for parasite resistance and susceptible Suffolk lambs. Growth, feeding behavior, and feed efficiency (RFI. F:G) were evaluated during artificial Hc infection. Katahdin ewes from the Virginia …

Machine Learning Approach To Health Risk Determination, Levi Butcher Cole

Graduate Theses, Dissertations, and Problem Reports (ETD)

In the United States, cardiovascular disease is the leading cause of death for both men and women. The factors that contribute to heart disease are well known but it is still an ever-evolving field of research. The possible benefits to being able to determine an individual’s Health Risk could provide the early insights needed to combat cardiovascular disease. However, two major problems within this area are the public availability of a dataset and the label imbalances for the dataset. In this thesis, we present a framework for exploring health risk using a combination of the NHANES dataset and open-source machine …

Vaping During Pregnancy: Effects On Vascular And Behavioral Outcomes In Offspring, Eiman A. Aboaziza

Graduate Theses, Dissertations, and Problem Reports (ETD)

Electronic cigarette (e-cig) use is increasing due to aggressive marketing, tempting flavors, and seemingly higher acceptability in the community (lesser perceived social stigma) despite unproven claims of safety. In an alarming trend, pregnant women smokers have turned to novel “modified risk” products, such as e-cigs, in response to heavy marketing of e-cigs as safer alternatives to cigarettes and a tool to help quit smoking. This is despite proven detrimental effects of nicotine on a growing fetus, and scarcity of information regarding toxicity of e-liquid (with and without nicotine) on child development. Moreover, rampant e-cig use among youth (nearly 4 million …

Evaluate The Efficacy Of A Mixture Of Peroxyacetic Acid And H2o2 Against The Survival And Cross-Contamination Of The Salmonella Surrogate Enterococcus Faecium On Tomatoes During Triple-Wash, Corey Waitman Coe

Graduate Theses, Dissertations, and Problem Reports (ETD)

Triple-wash with a mixture of peroxyacetic acid and H2O2 (SaniDate-5.0) during post-harvest processing of fresh produce has been recommended by West Virginia Small Farm Center to improve microbial safety. It has been well recognized that the washing of produce is more important for preventing cross-contamination than reducing foodborne pathogens. Furthermore, it may help improve public confidence in that the produce they obtain from locally grown farmers is safe for their consumption. determine the efficacy of SaniDate-5.0 for reducing the survival and preventing cross-contamination of the Salmonella surrogate Enterococcus faecium on tomatoes during triple-wash.

E. faecium ATCC-8459 (resistant to 100-ppm nalidixic-acid) …

Exploiting Modulation Of The Blood-Brain And Blood-Tumor Barrier Permeability By Translational Focused Ultrasound For Therapeutic Delivery To Cns Metastases, Tasneem A. Arsiwala

Graduate Theses, Dissertations, and Problem Reports (ETD)

Transcranial low-intensity focused ultrasound is a unique technology to modulate the integrity of tight endothelial junctions and transiently increase BBB/BTB permeability to enhance therapeutic delivery. Despite promising early studies, present literature lacks agreement on key experimental conditions, which restricts our knowledge and the technique's widespread translation. This dissertation first provides a critical review of the current gaps in knowledge regarding the universal use of LiFUS in preclinical and clinical use. We then identify key parameters for translational and predictable opening of the BBB using a 3T MRI coupled with a clinical device. Our investigation highlights that passive permeability of the …

The Relationship Between Oral Health And Health-Related Quality Of Life Among Hemodialysis Patients In Rural West Virginia, Kimberly A. Wallace

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction: Approximately 786,000 Americans are currently diagnosed with end-stage renal disease, 71% of whom are undergoing hemodialysis (HD) treatment. There is a bidirectional relationship between chronic kidney disease and periodontal disease. Among HD patients, poor oral health is prominent and can negatively impact general health and health-related quality of life. Appalachian residents experience extreme health and poverty inequities and limited access to healthcare and dental providers.

Method: The study was designed based on the Andersen Behavioral Model of Health Services Use. 120 participants from 6 outpatient HD centers were enrolled. Data were collected using a 34-item questionnaire including oral health …

An Investigation Into The Mechanism Of Proteasome Dysfunction In Neurodegenerative Disease And The Biological Impact Of Proteasome Hyperactivation In C. Elegans, Raymond T. Anderson

Graduate Theses, Dissertations, and Problem Reports (ETD)

Aging is an inevitable process that occurs as humans grow older. It is characterized by the chronological accumulation of cellular damage over time leading to functional decline as an organism grows older. Several processes are thought to contribute to the aging phenomenon, but one of the most prolific of these is the disruption of protein homeostasis (proteostasis). The collapse of proteostasis can lead to accelerated aging and the development of age-related diseases including devastating neurodegenerative diseases (NDs) like Alzheimer and Parkinson disease. Virtually all NDs are characterized by the buildup of proteins in and around neurons resulting in neuronal death …

Smallmouth Bass (Micropterus Dolomieu) Population Characteristics And An Evaluation Of Management Responses In Two West Virginia Watersheds, Jacob D. Harrell

Graduate Theses, Dissertations, and Problem Reports (ETD)

Smallmouth Bass (Micropterus dolomieu) population characteristics were examined within the Tygart and Cheat River watersheds of north-central West Virginia. Growth, mortality and recruitment were examined for each individual population prior to modeling potential effects of various management schemes. Smallmouth Bass were sampled for total length and weight, aged using sagittal otoliths and sexed through observations of gonadal or ovarian development. Growth for each population was examined using von Bertalanffy growth models and assessed for variation among populations. Population demographic characteristics differed between and among populations, most noticeably between riverine and lacustrine habitat, whereby growth, mortality and recruitment were higher …

Needs Assessment Of Diabetes Self-Management Education And Support Within West Virginia From Provider Viewpoints, Kasey M. Yost

Graduate Theses, Dissertations, and Problem Reports (ETD)

Diabetes is a chronic health condition that affects millions of Americans. It can have significant, lasting effects on an individual’s mental and physical wellbeing, especially when it goes unmanaged or untreated. Diabetes has been a growing problem in West Virginia (WV), which has the highest adult rate of diabetes at 15.7% in the US. Furthermore, WV has the highest mortality rate associated with diabetes at 36.2%. An effective method of management is through diabetes self-management education and support (DSMES). DSMES is a critical component of care for all individuals with diabetes. It is defined as the ongoing process of facilitating …

Global Engagement Model For Land-Grant Universities, A Grounded Theory., Lorena Ivonne Ballester

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of this study is to examine how land-grant universities engage with the public in the era of globalization; to explain the institutionalization of engagement processes accounting for the global context; and, to integrate this analysis into a Global Engagement Model for Land-Grant Universities (GEM) implementing a grounded theory research methodology.

GEM’s foundation is both theoretical and empirical. Applying the intensity sampling method, the universities selected for the empirical base were: The University of California, Davis, Michigan State University, and The Pennsylvania State University. Rather than an exact representation of individual universities’ engagement models, the model is the researcher’s …

The Mercury 2022

The Mercury

No abstract provided.