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Student Preferences In Using Affordable Learning Materials To Teach Research Methods Online, Phillip D. Grant, Elizabeth M. Pope Jan 2022

Student Preferences In Using Affordable Learning Materials To Teach Research Methods Online, Phillip D. Grant, Elizabeth M. Pope

International Journal for the Scholarship of Teaching & Learning

This basic qualitative study assessed students’ perceptions of affordable learning materials in an asynchronous master’s level research methods course at a regional university. Students preferred instructor-created mini-lectures (45%) more than required readings (39%), supplementary items concerning a specific topic (7%), sample studies (5%), CITI training modules (3%), and continuing education materials (2%). We found that students tended to prefer material that offered foundational information about research methods. Students also preferred material that “translated” academic language. Students preferred materials that were compatible with their perceived learning style. Finally, students preferred materials that related research methods to their own professional practice.


Influence Of Discussion Prompts On Fostering Student Teachers’ Competence In Knowledge Of English Instruction, Chin-Wen Chien Jan 2022

Influence Of Discussion Prompts On Fostering Student Teachers’ Competence In Knowledge Of English Instruction, Chin-Wen Chien

International Journal for the Scholarship of Teaching & Learning

This study analyzed questionnaire, pre-test, post-test, and online posts to explore influence of discussion prompts on the competence in English instruction of seven student teachers of elementary school EFL (English as a Foreign Language) in a practicum in Taiwan. This study reached the following conclusions. First, student teachers had good perceptions of the discussion prompts, particularly their effects on reflection on and relevance of English instruction. Secondly, student teachers had good perceptions of discussion prompts because of their clarity; however, they were not satisfied with inactive discussions among participants. Finally, participants’ education background and teaching experience affected their perceptions and …


Psychopathy And Emotion Regulation: The Mediating Role Of Motives And Goals, Morning S. Baker Jan 2022

Psychopathy And Emotion Regulation: The Mediating Role Of Motives And Goals, Morning S. Baker

Dissertations and Theses @ UNI

The purpose of this study was to examine the relation between psychopathy and emotion regulation as serially mediated by emotional motives and goals in an all-male sample. Six serial mediation models were proposed, three for each motive-type (i.e., hedonistic, instrumental), and two for each emotion (i.e., anger, fear, joy). Eight-hundred and seventy-eight participants were recruited via Amazon’s Mechanical Turk (MTurk). Results indicate that male MTurk workers with greater levels of psychopathy have poorer emotion regulation, emotion goals for experiencing anger and fear, hedonistic and instrumental motives for experiencing anger and fear, emotion goals for not experiencing joy, and hedonistic and …


Machine Learning Land Cover And Land Use Classification Of 4-Band Satellite Imagery, Lorelei Turner, Torrey J. Wagner, Paul Auclair, Brent T. Langhals Jan 2022

Machine Learning Land Cover And Land Use Classification Of 4-Band Satellite Imagery, Lorelei Turner, Torrey J. Wagner, Paul Auclair, Brent T. Langhals

Faculty Publications

Land-cover and land-use classification generates categories of terrestrial features, such as water or trees, which can be used to track how land is used. This work applies classical, ensemble and neural network machine learning algorithms to a multispectral remote sensing dataset containing 405,000 28x28 pixel image patches in 4 electromagnetic frequency bands. For each algorithm, model metrics and prediction execution time were evaluated, resulting in two families of models; fast and precise. The prediction time for an 81,000-patch group of predictions wasmodels, and >5s for the precise models, and there was not a significant change in prediction time when a …


Africana Legal Studies: A New Theoretical Approach To Law & Protocol, Angi Porter Jan 2022

Africana Legal Studies: A New Theoretical Approach To Law & Protocol, Angi Porter

Scholarly Articles in Law Reviews & Journals

INTRODUCTION: In 1743, a group of enslaved Africans from various estates in French colonial New Orleans gathered, held a musical ceremony sung in their native language, and discussed the actions and fate of a slaveholder named Corbin. Earlier, Corbin had threatened to shoot one of the enslaved Africans in this group, and Corbin’s brother then actually shot that person with a gun loaded with salt. Now, as the group of Africans gathered, they determined that Corbin had to die. Two months later, Corbin disappeared and was never found.

If we use a traditional (Western) legal framework to describe this …


Review Of Age-Related Trends Of Infection Among People Hospitalized For Infection With Concurrent Substance Use, Julia Catherine Lucia Gregorio Jan 2022

Review Of Age-Related Trends Of Infection Among People Hospitalized For Infection With Concurrent Substance Use, Julia Catherine Lucia Gregorio

Honors Theses and Capstones

Abstract

Background: Opioids and related injections are the most common drug associated with infection (Capizzi et al., 2020). Overall, data overwhelmingly shows infection and overdose rates on the rise in recent years (Kaiser Family Foundation, 2019).

Methods: The aim of this study was to identify trends between age and concurrent infection rates related to substance use from 2012 to 2019. This quantitative study utilized data from the NH Hospital Discharge Summaries (Office of Health Statistics, 2021). Specifically, trends among three age groups (young adults (18-34), middle-aged (35-54), and older adults (55+)) who were admitted to an inpatient unit or the …


Western Herbalism: What Drives Individuals Within Rhode Island To Study This Ancient Healing Modality?, Kyla Shea Jan 2022

Western Herbalism: What Drives Individuals Within Rhode Island To Study This Ancient Healing Modality?, Kyla Shea

Student Publications

People globally rely on herbal practices as their main form of healing, yet few people in the West understand how to properly identify and effectively use herbs. This loss of knowledge regarding traditional healing practices was done so intentionally during enslavement and colonization, resulting in exploitation and misinterpretation of herbal practices. By reclaiming this knowledge, it is not only a way to improve the health of communities and the land, but it is also a way of reclaiming ancestral traditions and connection with oneself. Furthermore, this research paper explores the complexities of Western herbalism and determines what drives individuals within …


Electronic Word Of Mouth, Online Advertisement Value And Attitude Toward Egyptian Websites As Antecedents Of Online Purchase Intention: An Extended Technology Acceptance Model, Mayada Aref Jan 2022

Electronic Word Of Mouth, Online Advertisement Value And Attitude Toward Egyptian Websites As Antecedents Of Online Purchase Intention: An Extended Technology Acceptance Model, Mayada Aref

Faculty Journal Articles

The understanding of the antecedent of online purchase intentions is imperative for the growth of electronic commerce. This paper extended the technology acceptance model by three factors electronic word of mouth, online advertisement value, and attitude toward Egyptian websites, and empirically examined the proposed model among Egyptian Internet users. Furthermore, based on online purchase intention, market segmentation was conducted. A structured online questionnaire was used to collect 479 responses. Explanatory factor analysis and cluster analysis were performed using SPSS software; the structural equation modeling and hypotheses testing were examined using Amos IBM. The paper contributed by identifying the main factors …


Channa Reddy In Telangana Statehood Movement: Initiation, Intervention And Contribution, Jakkula Koteswar Rao, Ram Shepherd Bheenaveni Jan 2022

Channa Reddy In Telangana Statehood Movement: Initiation, Intervention And Contribution, Jakkula Koteswar Rao, Ram Shepherd Bheenaveni

School of Social Work Faculty Publications

The present paper tries to explore some of the repeated accusations and half-truths against the Telangana leader – Dr. Marri Channa Reddy, who led the movement in 1969. It is believed that the leaders started the Telangana Statehood movement as a problem-solving movement against subregionalism. Dr. Reddy was a staunch opponent of Andhra’s internal colonization. This is not only absurd but also a parody of the truth. This is mainly due to the lack of original documents in an organized campaign for the separate state of Telangana. The print and electronic media reports speak of alarming national integration at the …


Credit, Crises And Infrastructure: The Differing Fates Of Large And Small Businesses, Todd Baker, Kathryn Judge, Aaron Klein Jan 2022

Credit, Crises And Infrastructure: The Differing Fates Of Large And Small Businesses, Todd Baker, Kathryn Judge, Aaron Klein

Faculty Scholarship

This Essay sheds new light on the importance of credit creation infrastructure in determining who actually receives government support during periods of distress, and who continues to benefit after the acute phase of a crisis and the government’s formal support programs come to an end. The pandemic revealed, and the government’s response accentuated, meaningful asymmetries in the capacities of small and large businesses to access needed funding.

At first glance, it would seem that small businesses benefitted more than large ones from the government’s pandemic-support programs, as more government funds flowed into small businesses. Yet closer inspection of the range …


The State Of Our Community Social Capital In Kensington, New Hampshire, Sawyer B. Rogers Jan 2022

The State Of Our Community Social Capital In Kensington, New Hampshire, Sawyer B. Rogers

Honors Theses and Capstones

This study investigates the transformation of social capital over time, using the example of a small New England town: Kensington, New Hampshire. National social capital assessments indicate a substantial decline in social capital since the post-WW2 era. Kensington does not follow this extreme decline in social capital, with a significant rise during the 90s and into the early 2000s. Additionally, Kensington has high levels of social capital when compared to New Hampshire residents overall. Survey findings point to strong trust, yet weak social infrastructure in Kensington. Therefore, the most consistent way to maintain social capital between shifts is to create …


Il-17 Producing Lymphocytes Cause Dry Eye And Corneal Disease With Aging In Rxrα Mutant Mouse, Jehan Alam, Ghasem Yazdanpanah, Rinki Ratnapriya, Nicholas Borcherding, Cintia S De Paiva, Dequan Li, Rodrigo Guimaraes De Souza, Zhiyuan Yu, Stephen C Pflugfelder Jan 2022

Il-17 Producing Lymphocytes Cause Dry Eye And Corneal Disease With Aging In Rxrα Mutant Mouse, Jehan Alam, Ghasem Yazdanpanah, Rinki Ratnapriya, Nicholas Borcherding, Cintia S De Paiva, Dequan Li, Rodrigo Guimaraes De Souza, Zhiyuan Yu, Stephen C Pflugfelder

Faculty, Staff and Students Publications

PURPOSE: To investigate IL-17 related mechanisms for developing dry eye disease in the Pinkie mouse strain with a loss of function RXRα mutation.

METHODS: Measures of dry eye disease were assessed in the cornea and conjunctiva. Expression profiling was performed by single-cell RNA sequencing (scRNA-seq) to compare gene expression in conjunctival immune cells. Conjunctival immune cells were immunophenotyped by flow cytometry and confocal microscopy. The activity of RXRα ligand 9-cis retinoic acid (RA) was evaluated in cultured monocytes and γδ T cells.

RESULTS: Compared to wild type (WT) C57BL/6, Pinkie has increased signs of dry eye disease, including decreased tear …


Impact Of Covid-19 On Intensive Care Unit Nurse Duty Of Care And Professional Roles: A Qualitative Content Analysis, Jacqueline Christianson, Jill L. Guttormson, Natalie S. Mcandrew, Kelly Calkins Jan 2022

Impact Of Covid-19 On Intensive Care Unit Nurse Duty Of Care And Professional Roles: A Qualitative Content Analysis, Jacqueline Christianson, Jill L. Guttormson, Natalie S. Mcandrew, Kelly Calkins

College of Nursing Faculty Research and Publications

Background

Nurse duty of care, the balance between nursing occupational obligations to provide care, the personal costs for providing such care, and the reward for providing care, has been significantly altered by the COVID-19 pandemic. ICU nurses are increasingly burdened with higher personal costs to fulfill their jobs, but little additional reward for continuing to provide care.

Objectives

The purpose of this study was to examine the impact of the COVID-19 pandemic on the duty of care balance among ICU nurses who manage COVID-19 patients.

Design

This was a descriptive qualitative study using semi-structured interviews.

Methods

Nurses were recruited for …


Estimation Of Intrafamilial Dna Contamination In Family Trio Genome Sequencing Using Deviation From Mendelian Inheritance, Christopher J Yoon, Brian Wiley, Malachi Griffith, Obi L Griffith, Et Al. Jan 2022

Estimation Of Intrafamilial Dna Contamination In Family Trio Genome Sequencing Using Deviation From Mendelian Inheritance, Christopher J Yoon, Brian Wiley, Malachi Griffith, Obi L Griffith, Et Al.

2020-Current year OA Pubs

With the increasing number of sequencing projects involving families, quality control tools optimized for family genome sequencing are needed. However, accurately quantifying contamination in a DNA mixture is particularly difficult when genetically related family members are the sources. We developed TrioMix, a maximum likelihood estimation (MLE) framework based on Mendel's law of inheritance, to quantify DNA mixture between family members in genome sequencing data of parent-offspring trios. TrioMix can accurately deconvolute any intrafamilial DNA contamination, including parent-offspring, sibling-sibling, parent-parent, and even multiple familial sources. In addition, TrioMix can be applied to detect genomic abnormalities that deviate from Mendelian inheritance patterns, …


Variation In Ampicillin Dosing For Lower Respiratory Tract Infections And Neonatal Bacterial Infections In Us Children's Hospitals, Elizabeth A Daniels, Christopher C Mcpherson, Jason G Newland, Brian R Lee Jan 2022

Variation In Ampicillin Dosing For Lower Respiratory Tract Infections And Neonatal Bacterial Infections In Us Children's Hospitals, Elizabeth A Daniels, Christopher C Mcpherson, Jason G Newland, Brian R Lee

2020-Current year OA Pubs

OBJECTIVE: We examined ampicillin dosing in pediatric patients across 3 conditions: (1) bacterial lower respiratory tract infections (LRTIs) in infants and children >3 months, (2) neonates with suspected or proven sepsis, and (3) neonates with suspected central nervous system (CNS) infections. We compared our findings to dosing guidance for these specific indications.

DESIGN: Retrospective cohort study.

SETTING: The study included data from 32 children's hospitals in the United States.

METHODS: We reviewed prescriptions from the SHARPS study of antimicrobials, a survey of antibiotic prescribing from July 2016 to December 2017. Prescriptions were analyzed for indication, total daily dose per kilogram, …


Self-Esteem And Weight Status Of Young Adults: Findings From A Pilot Study, Echu Liu, Su-Hsin Chang Jan 2022

Self-Esteem And Weight Status Of Young Adults: Findings From A Pilot Study, Echu Liu, Su-Hsin Chang

2020-Current year OA Pubs

BACKGROUND: Researchers have examined the association between self-esteem and obesity in past studies. However, most studies have focused on pediatric or adolescent populations. In this pilot study, we aim to explore the association of self-esteem with weight status in young adults, a group of individuals in a significant and pivotal period of their lives whose needs and challenges have received little attention in previous studies.

MATERIALS AND METHODS: Exactly 127 college juniors and seniors in the Midwest participated in late 2017. For our survey, we collected the following information: body weight, height, self-esteem score from the state self-esteem scale, grade …


Follistatin Protein Enhances Satellite Cell Counts In Reinnervated Muscle, Mark A. Feger, Jonathan Isaacs, Satya Mallu, Dorne Yager, Mary Shall, Gaurangkumar Patel, Omar Protzuk, Akhil S Bokkisam Jan 2022

Follistatin Protein Enhances Satellite Cell Counts In Reinnervated Muscle, Mark A. Feger, Jonathan Isaacs, Satya Mallu, Dorne Yager, Mary Shall, Gaurangkumar Patel, Omar Protzuk, Akhil S Bokkisam

2020-Current year OA Pubs

No abstract provided.


Distinguishing Tumor Admixed In A Radiation Necrosis (Rn) Background: 1h And 2h Mr With A Novel Mouse Brain-Tumor/Rn Model, Xia Ge, Kyu-Ho Song, John A Engelbach, Liya Yuan, Feng Gao, Sonika Dahiya, Keith M Rich, Joseph J H Ackerman, Joel R Garbow Jan 2022

Distinguishing Tumor Admixed In A Radiation Necrosis (Rn) Background: 1h And 2h Mr With A Novel Mouse Brain-Tumor/Rn Model, Xia Ge, Kyu-Ho Song, John A Engelbach, Liya Yuan, Feng Gao, Sonika Dahiya, Keith M Rich, Joseph J H Ackerman, Joel R Garbow

2020-Current year OA Pubs

PURPOSE: Distinguishing radiation necrosis (RN) from recurrent tumor remains a vexing clinical problem with important health-care consequences for neuro-oncology patients. Here, mouse models of pure tumor, pure RN, and admixed RN/tumor are employed to evaluate hydrogen (

MATERIALS AND METHODS: A pipeline of common quantitative

RESULTS: Differences in quantitative

CONCLUSIONS: These findings, employing a pipeline of quantitative


Diabetic Retinopathy As A Predictor Of Cardiovascular Morbidity And Mortality In Subjects With Type 2 Diabetes, Joan Barrot, Jordi Real, Bogdan Vlacho, Pedro Romero-Aroca, Rafael Simó, Didac Mauricio, Manel Mata-Cases, Esmeralda Castelblanco, Xavier Mundet-Tuduri, Josep Franch-Nadal Jan 2022

Diabetic Retinopathy As A Predictor Of Cardiovascular Morbidity And Mortality In Subjects With Type 2 Diabetes, Joan Barrot, Jordi Real, Bogdan Vlacho, Pedro Romero-Aroca, Rafael Simó, Didac Mauricio, Manel Mata-Cases, Esmeralda Castelblanco, Xavier Mundet-Tuduri, Josep Franch-Nadal

2020-Current year OA Pubs

This study aimed to evaluate the predictive value of diabetic retinopathy (DR) and its stages with the incidence of major cardiovascular events and all-cause mortality in type 2 diabetes mellitus (T2DM) persons in our large primary healthcare database from Catalonia (Spain). A retrospective cohort study with pseudo-anonymized routinely collected health data from SIDIAP was conducted from 2008 to 2016. We calculated incidence rates of major cardiovascular events [coronary heart disease (CHD), stroke, or both-macrovascular events] and all-cause mortality for subjects with and without DR and for different stages of DR. The proportional hazards regression analysis was done to assess the …


Low Cancer Incidence In Naked Mole-Rats May Be Related To Their Inability To Express The Warburg Effect, Pedro Freire Jorge, Matthew L Goodwin, Maurits H Renes, Maarten W Nijsten, Matthew Pamenter Jan 2022

Low Cancer Incidence In Naked Mole-Rats May Be Related To Their Inability To Express The Warburg Effect, Pedro Freire Jorge, Matthew L Goodwin, Maurits H Renes, Maarten W Nijsten, Matthew Pamenter

2020-Current year OA Pubs

Metabolic flexibility in mammals enables stressed tissues to generate additional ATP by converting large amounts of glucose into lactic acid; however, this process can cause transient local or systemic acidosis. Certain mammals are adapted to extreme environments and are capable of enhanced metabolic flexibility as a specialized adaptation to challenging habitat niches. For example, naked mole-rats (NMRs) are a fossorial and hypoxia-tolerant mammal whose metabolic responses to environmental stressors markedly differ from most other mammals. When exposed to hypoxia, NMRs exhibit robust hypometabolism but develop minimal acidosis. Furthermore, and despite a very long lifespan relative to other rodents, NMRs have …


Prescribing Unproven Cancer Drugs: Physician Perspectives On Expanded Access And Right To Try, Haley Manley, Bryan A Sisk, Zubin Master, Christopher Thomas Scott Jan 2022

Prescribing Unproven Cancer Drugs: Physician Perspectives On Expanded Access And Right To Try, Haley Manley, Bryan A Sisk, Zubin Master, Christopher Thomas Scott

2020-Current year OA Pubs

BACKGROUND: For gravely ill patients who have no treatment options and who are ineligible for clinical trials, the US Food and Drug Administration (FDA) established the Expanded Access Program (EAP). Motivated by efforts to weaken FDA regulation and sold as providing greater access to experimental drugs, the federal Right to Try Act (RTT) was passed in 2017. It reduces FDA oversight by not requiring physicians to report safety data and foregoes approval of protocols by local institutional review boards.

METHODS: This study explored the views of 17 neuro-oncologists from 15 different academic medical centers with varying experience with EAP and …


Understanding The Contribution Of Metabolism To Mycobacterium Tuberculosis Drug Tolerance, Amanda N Samuels, Erin R Wang, Gregory A Harrison, Joy C Valenta, Christina L Stallings Jan 2022

Understanding The Contribution Of Metabolism To Mycobacterium Tuberculosis Drug Tolerance, Amanda N Samuels, Erin R Wang, Gregory A Harrison, Joy C Valenta, Christina L Stallings

2020-Current year OA Pubs

Treatment of


Analysis Of Neuronal Injury Transcriptional Response Identifies Ctcf And Yy1 As Co-Operating Factors Regulating Axon Regeneration, Oshri Avraham, Jimmy Le, Kathleen Leahy, Tiandao Li, Guoyan Zhao, Valeria Cavalli Jan 2022

Analysis Of Neuronal Injury Transcriptional Response Identifies Ctcf And Yy1 As Co-Operating Factors Regulating Axon Regeneration, Oshri Avraham, Jimmy Le, Kathleen Leahy, Tiandao Li, Guoyan Zhao, Valeria Cavalli

2020-Current year OA Pubs

Injured sensory neurons activate a transcriptional program necessary for robust axon regeneration and eventual target reinnervation. Understanding the transcriptional regulators that govern this axon regenerative response may guide therapeutic strategies to promote axon regeneration in the injured nervous system. Here, we used cultured dorsal root ganglia neurons to identify pro-regenerative transcription factors. Using RNA sequencing, we first characterized this neuronal culture and determined that embryonic day 13.5 DRG (eDRG) neurons cultured for 7 days are similar to e15.5 DRG neurons


Binary Acoustic Metasurfaces For Dynamic Focusing Of Transcranial Ultrasound, Zhongtao Hu, Yaoheng Yang, Lu Xu, Yao Hao, Hong Chen Jan 2022

Binary Acoustic Metasurfaces For Dynamic Focusing Of Transcranial Ultrasound, Zhongtao Hu, Yaoheng Yang, Lu Xu, Yao Hao, Hong Chen

2020-Current year OA Pubs

Transcranial focused ultrasound (tFUS) is a promising technique for non-invasive and spatially targeted neuromodulation and treatment of brain diseases. Acoustic lenses were designed to correct the skull-induced beam aberration, but these designs could only generate static focused ultrasound beams inside the brain. Here, we designed and 3D printed binary acoustic metasurfaces (BAMs) for skull aberration correction and dynamic ultrasound beam focusing. BAMs were designed by binarizing the phase distribution at the surface of the metasurfaces. The phase distribution was calculated based on time reversal to correct the skull-induced phase aberration. The binarization enabled the ultrasound beam to be dynamically steered …


Program Adaptation By Health Departments, Louise Farah Saliba, Peg Allen, Stephanie L Mazzucca, Emily Rodriguez Weno, Sarah Moreland-Russell, Margaret Padek, Ross C Brownson Jan 2022

Program Adaptation By Health Departments, Louise Farah Saliba, Peg Allen, Stephanie L Mazzucca, Emily Rodriguez Weno, Sarah Moreland-Russell, Margaret Padek, Ross C Brownson

2020-Current year OA Pubs

INTRODUCTION: The dissemination of evidence-based interventions (i.e., programs, practices, and policies) is a core function of US state health departments (SHDs). However, interventions are originally designed and tested with a specific population and context. Hence, adapting the intervention to meet the real-world circumstances and population's needs can increase the likelihood of achieving the expected health outcomes for the target population from the implemented intervention. This study identified how SHD employees decide to adapt public health programs and what influences decisions on how to adapt them.

MATERIALS AND METHODS: SHD employees (

RESULTS: Data, outcomes, and health department evaluations influenced decisions …


Bridging The Technological Divide: Stigmas And Challenges With Technology In Digital Brain Health Studies Of Older Adults, Jessica Nicosia, Andrew J Aschenbrenner, Sarah L Adams, Marisol Tahan, Sarah H Stout, Hannah Wilks, Joyce E Balls-Berry, John C Morris, Jason Hassenstab Jan 2022

Bridging The Technological Divide: Stigmas And Challenges With Technology In Digital Brain Health Studies Of Older Adults, Jessica Nicosia, Andrew J Aschenbrenner, Sarah L Adams, Marisol Tahan, Sarah H Stout, Hannah Wilks, Joyce E Balls-Berry, John C Morris, Jason Hassenstab

2020-Current year OA Pubs

The COVID-19 pandemic has increased adoption of remote assessments in clinical research. However, longstanding stereotypes persist regarding older adults' technology familiarity and their willingness to participate in technology-enabled remote studies. We examined the validity of these stereotypes using a novel technology familiarity assessment (


Fluorescence Imaging Of Beta Cell Primary Cilia, Zipeng A Li, Jung Hoon Cho, Louis G Woodhams, Jing W Hughes Jan 2022

Fluorescence Imaging Of Beta Cell Primary Cilia, Zipeng A Li, Jung Hoon Cho, Louis G Woodhams, Jing W Hughes

2020-Current year OA Pubs

Primary cilia are slender cell-surface organelles that project into the intercellular space. In pancreatic beta cells, primary cilia coordinate a variety of cell responses including GPCR signaling, calcium influx, and insulin secretion, along with likely many underappreciated roles in islet development and differentiation. To study cilia function in islet biology, direct visualization of primary cilia by microscopic methods is often a necessary first step. Ciliary abundance, distribution, and morphology are heterogeneous among islet cells and are best visualized by fluorescence microscopy, the tools for which are readily accessible to most researchers. Here we present a collection of fluorescence imaging methods …


Actin Capping Protein Regulates Postsynaptic Spine Development Through Cpi-Motif Interactions, Kenneth R Myers, Yanjie Fan, Patrick Mcconnell, John A Cooper, James Q Zheng Jan 2022

Actin Capping Protein Regulates Postsynaptic Spine Development Through Cpi-Motif Interactions, Kenneth R Myers, Yanjie Fan, Patrick Mcconnell, John A Cooper, James Q Zheng

2020-Current year OA Pubs

Dendritic spines are small actin-rich protrusions essential for the formation of functional circuits in the mammalian brain. During development, spines begin as dynamic filopodia-like protrusions that are then replaced by relatively stable spines containing an expanded head. Remodeling of the actin cytoskeleton plays a key role in the formation and modification of spine morphology, however many of the underlying regulatory mechanisms remain unclear. Capping protein (CP) is a major actin regulating protein that caps the barbed ends of actin filaments, and promotes the formation of dense branched actin networks. Knockdown of CP impairs the formation of mature spines, leading to …


One Vaccine To Counter Many Diseases? Modeling The Economics Of Oral Polio Vaccine Against Child Mortality And Covid-19, Angela Y Chang, Peter Aaby, Michael S Avidan, Christine S Benn, Stefano M Bertozzi, Lawrence Blatt, Konstantin Chumakov, Shabaana A Khader, Shyam Kottilil, Madhav Nekkar, Mihai G Netea, Annie Sparrow, Dean T Jamison Jan 2022

One Vaccine To Counter Many Diseases? Modeling The Economics Of Oral Polio Vaccine Against Child Mortality And Covid-19, Angela Y Chang, Peter Aaby, Michael S Avidan, Christine S Benn, Stefano M Bertozzi, Lawrence Blatt, Konstantin Chumakov, Shabaana A Khader, Shyam Kottilil, Madhav Nekkar, Mihai G Netea, Annie Sparrow, Dean T Jamison

2020-Current year OA Pubs

INTRODUCTION: Recent reviews summarize evidence that some vaccines have heterologous or non-specific effects (NSE), potentially offering protection against multiple pathogens. Numerous economic evaluations examine vaccines' pathogen-specific effects, but less than a handful focus on NSE. This paper addresses that gap by reporting economic evaluations of the NSE of oral polio vaccine (OPV) against under-five mortality and COVID-19.

MATERIALS AND METHODS: We studied two settings: (1) reducing child mortality in a high-mortality setting (Guinea-Bissau) and (2) preventing COVID-19 in India. In the former, the intervention involves three annual campaigns in which children receive OPV incremental to routine immunization. In the latter, …


Environmentally-Triggered Contraction Of The Norovirus Virion Determines Diarrheagenic Potential, Emily W Helm, Elizabeth A Kennedy, Megan T Baldridge, Et Al. Jan 2022

Environmentally-Triggered Contraction Of The Norovirus Virion Determines Diarrheagenic Potential, Emily W Helm, Elizabeth A Kennedy, Megan T Baldridge, Et Al.

2020-Current year OA Pubs

Noroviruses are the leading cause of severe childhood diarrhea and foodborne disease worldwide. While they are a major cause of disease in all age groups, infections in the very young can be quite severe with annual estimates of 50,000-200,000 fatalities in children under 5 years old. In spite of the remarkable disease burden associated with norovirus infections in people, very little is known about the pathogenic mechanisms underlying norovirus diarrhea, principally because of the lack of tractable small animal models. We recently demonstrated that wild-type neonatal mice are susceptible to murine norovirus (MNV)-induced acute self-resolving diarrhea in a time course …