Digital Commons Network^™

Isolation And Characterization Of Chi-Like Salmonella Bacteriophages Infecting Two Salmonella Enterica Serovars, Typhimurium And Enteritidis, Addisu D. Teklemariam, Mona G. Alharbi, Rashad R. Al-Hindi, Ibrahim Alotibi, Abdullah A. Aljaddawi, Sheren A. Azhari, Ahmed Esmael

Nebraska Center for Virology: Faculty Publications

Salmonella enterica Serovar Typhimurium and Salmonella enterica Serovar Enteritidis are well-known pathogens that cause foodborne diseases in humans. The emergence of antibiotic-resistant Salmonella serovars has caused serious public health problems worldwide. In this study, two lysogenic phages, STP11 and SEP13, were isolated from a wastewater treatment plant in Jeddah, KSA. Transmission electron microscopic images revealed that both phages are new members of the genus “Chivirus” within the family Siphoviridae. Both STP11 and SEP13 had a lysis time of 90 min with burst sizes of 176 and 170 PFU/cell, respectively. The two phages were thermostable (0 ^◦C …

Chemical Control And Understanding Of Horizontal Gene Transfers, Drug-Resistance Development, And Filament And Biofilm Formation, Yuchen Jin

Dissertations - ALL

Biofilms formed by microbes on surfaces are the sources for persistent infectious diseases and environmental problems. The mechanism and details of how antibiotics promote biofilm formation is largely unknown. For instance, it not clear what stages of biofilm growth are promoted to proceed faster than without antibiotics, what phenotypes of bacteria form in an antibiotic-promoted biofilm, and what different biofilm compositions and structures are caused by the presence of antibiotics. Among other effects, antibiotics can cause bacteria to form filaments of living bacteria. Here, we conduct a real-time study of the adherence of bacteria and antibiotic-induced filamentous bacteria on surfaces …

Historical And Recent Breeding Of The Western Grebe And Clark's Grebe In A Severely Impaired Ecosystem At Clear Lake, California, Floyd E. Hayes, Bryan J. Mcintosh, Dylan G. Turner, Douglas E. Weidemann

Monographs of the Western North American Naturalist

Clear Lake is a large (176.7-km²) natural lake in northern California, USA. An estimated 1000 pairs of Western Grebes (Aechmophorus occidentalis) and Clark’s Grebes (A. clarkii) nested on the lake prior to massive applications of the pesticide dichlorodiphenyldichloroethane (DDD) to kill the Clear Lake gnat (Chaoborus astictopus) during 1949–1957. Biomagnification of DDD was implicated as the cause of several mass mortality events of grebes and short-term reproductive failure in the 1950s, although other factors such as mercury (Hg) poisoning may have contributed. Successful nesting resumed in 1960 and increased gradually, with up …

A Unified Model Of Age-Related Cardiovascular Disease, Michael Fossel, Joe Bean, Nina Khera, Mikhail G Kolonin

Faculty, Staff and Student Publications

Despite progress in biomedical technologies, cardiovascular disease remains the main cause of mortality. This is at least in part because current clinical interventions do not adequately take into account aging as a driver and are hence aimed at suboptimal targets. To achieve progress, consideration needs to be given to the role of cell aging in disease pathogenesis. We propose a model unifying the fundamental processes underlying most age-associated cardiovascular pathologies. According to this model, cell aging, leading to cell senescence, is responsible for tissue changes leading to age-related cardiovascular disease. This process, occurring due to telomerase inactivation and telomere attrition, …

The Rheumatoid Arthritis Drug Auranofin Lowers Leptin Levels And Exerts Antidiabetic Effects In Obese Mice, Aaron R Cox, Peter M Masschelin, Pradip K Saha, Jessica B Felix, Robert Sharp, Zeqin Lian, Yan Xia, Natasha Chernis, David A Bader, Kang Ho Kim, Xin Li, Jun Yoshino, Xin Li, Gang Li, Zheng Sun, Huaizhu Wu, Cristian Coarfa, David D Moore, Samuel Klein, Kai Sun, Sean M Hartig

Faculty, Staff and Student Publications

Low-grade, sustained inflammation in white adipose tissue (WAT) characterizes obesity and coincides with type 2 diabetes mellitus (T2DM). However, pharmacological targeting of inflammation lacks durable therapeutic effects in insulin-resistant conditions. Through a computational screen, we discovered that the FDA-approved rheumatoid arthritis drug auranofin improved insulin sensitivity and normalized obesity-associated abnormalities, including hepatic steatosis and hyperinsulinemia in mouse models of T2DM. We also discovered that auranofin accumulation in WAT depleted inflammatory responses to a high-fat diet without altering body composition in obese wild-type mice. Surprisingly, elevated leptin levels and blunted beta-adrenergic receptor activity achieved by leptin receptor deletion abolished the antidiabetic …

An Artificial Intelligence (Ai) Model For Prediction Of Covid 19 Infection Risk In The Uae Population, Sara Alshaya, Sheik Abdullah Jamal Mohideen, Aji Gopakumar, Vibhor Mathur

The First Scientific Conference on Health and Medical Research in the UAE 5-6 December 2022

No abstract provided.

The Prevalence Of Sars-Cov-2 Antibodies Within The Community Of A Private Tertiary University In The Philippines: A Serial Cross Sectional Study, Lourdes S. Tanchanco, Jenica Clarisse Y. Sy, Angel Belle C. Dy, Myla D. Levantino, Arianna Maever L. Amit, John Wong, Kirsten Angeles, John Paul Vergara

Ateneo School of Medicine and Public Health Publications

The COVID-19 pandemic has caused a public health emergency in all sectors of society, including universities and other academic institutions. This study determined the seroprevalence of SARS-CoV-2 antibodies among administrators, faculty, staff, and students of a private tertiary academic institution in the Philippines over a 7 month period. It employed a serial cross-sectional method using qualitative and quantitative COVID-19 antibody test kits. A total of 1,318 participants were tested, showing 47.80% of the study population yielding IgG antibodies to SARS-CoV-2 virus. A general increase in seroprevalence was observed from June to December 2021, which coincided with the vaccine roll-out of …

Optimal Dose Of Vitamin D For Covid-19 Treatment, Dita Mega Utami, Muhammad Abdurrahman Rasyid Ash-Siddiq, Desi Rianti Rahmahani, Muhammad Iqbal Mubarok, Muhammad Zulkifly Tasman, Jeremy Nicolas Sibarani, Habibah Teniya Ariq Fauziyah, Budi Utomo, Shifa Fauziyah

Folia Medica Indonesiana

Highlights: • Vitamin D supplementations in different doses yield different outcomes. • Multi-day vitamin D administration of 1000-6000 IU in patients with COVID-19 has more positive impacts than a single high dose of vitamin D. • Patient morbidity, length of hospitalization, and patient mortality improved with multi-day vitamin D administration. Abstract: This meta-analysis aimed to determine whether there is any optimal dose of vitamin D for morbidity, length of hospitalization, and mortality in patients with COVID-19. We conducted a comprehensive search in three online databases for eligible studies until February 28, 2022. Odds ratio (OR) and standardized mean difference (SMD) …

Managing Tuberculosis In Refugee Populations, Roselyn Fierkens

Senior Honors Theses

Tuberculosis (TB) is an airborne-transmitted infectious disease, responsible for more global deaths than HIV. Multidrug-resistant strains are developing, which heightens the need for swift eradication. Current data states that 71.4% of all TB cases in the United States occur in foreign-born individuals, so populations traveling from TB endemic areas are a key target population for prevention strategies. Various screening procedures exist, but no successful standard is in place. Beyond prevention and treatment strategies, adaptations to cultural communication differences are integral to successful medical interventions. This synthesis of TB pathophysiology, treatments, risk factors, and cultural considerations is intended for use in …

Effect Of Secondary Metabolite Crude Of Metarhizum Anisopliea Fungus On The Second Larval Stage Of The Housefly Musca Domestica, Soolaf A Kathiar, Hazim Idan Al Shammari, Aliaa Abdul Aziz Hammed

Baghdad Science Journal

The house flies Musca domestica )Diptera:musidae) are the primary carrier of many pathogens such as cholera, typhoid, anthrax, and others. The use of chemical pesticides as a basic method of control leads to many problems at the environmental and health level. The use of safe alternatives to chemical pesticides has become an urgent necessity. The research aims to find biological alternatives that are environment-friendly and non-pathogenic to humans in controlling house flies through the possibility of extracting and diagnosing some secondary metabolites produced by the fungus Metarhizium anisopliae and testing their effects on the second larval stage of house flies …

Nursing Considerations For Elderly Adults With Urinary Tract Infections In Nursing Homes, Mary Marlett

D.U.Quark

No abstract provided.

Six-Dimensional Single-Molecule Imaging With Isotropic Resolution Using A Multi-View Reflector Microscope, Oumeng Zhang, Zijian Guo, Yuanyuan He, Tingting Wu, Michael D. Vahey, Matthew D. Lew

Electrical & Systems Engineering Publications and Presentations

Imaging of both the positions and orientations of single fluorophores, termed single-molecule orientation-localization microscopy, is a powerful tool for the study of biochemical processes. However, the limited photon budget associated with single-molecule fluorescence makes high-dimensional imaging with isotropic, nanoscale spatial resolution a formidable challenge. Here we realize a radially and azimuthally polarized multi-view reflector (raMVR) microscope for the imaging of the three-dimensional (3D) positions and 3D orientations of single molecules, with precisions of 10.9 nm and 2.0° over a 1.5-μm depth range. The raMVR microscope achieves 6D super-resolution imaging of Nile red molecules transiently bound to lipid-coated spheres, accurately resolving …

Exposure Of Egyptian Rousette Bats (Rousettus Aegyptiacus) And A Little Free-Tailed Bat (Chaerephon Pumilus) To Alphaviruses In Uganda, Rebekah C. Kading, Erin M. Borland, Eric C. Mossel, Teddy Nakayiki, Betty Nalikka, Jeremy P. Ledermann, Mary B. Crabtree, Nicholas A. Panella, Luke Nyakarahuka, Amy T. Gilbert, Julian C. Kerbis-Peterhans, Jonathan S. Towner, Brian R. Amman, Tara K. Sealy, Barry R. Miller, Julius J. Lutwama, Robert M. Kityo, Ann M. Powers

United States Department of Agriculture Wildlife Services: Staff Publications

The reservoir for zoonotic o’nyong-nyong virus (ONNV) has remained unknown since this virus was first recognized in Uganda in 1959. Building on existing evidence for mosquito bloodfeeding on various frugivorous bat species in Uganda, and seroprevalence for arboviruses among bats in Uganda, we sought to assess if serum samples collected from bats in Uganda demonstrated evidence of exposure to ONNV or the closely related zoonotic chikungunya virus (CHIKV). In total, 652 serum samples collected from six bat species were tested by plaque reduction neutralization test (PRNT) for neutralizing antibodies against ONNV and CHIKV. Forty out of 303 (13.2%) Egyptian rousettes …

Mortality Assessment Of Pediatric Septic Patients Through Pediatric Sofa+Anion Gap And Pelod-2 Scores, Johaan Pawe Siampa, Arie Utariani, Elizeus Hanindito

Folia Medica Indonesiana

Highlight: • Sepsis and septic shock cause morbidity and mortality in pediatric patients. • The accuracy of pediatric sequential organ failure assessment and anion gap (pSOFA+AG) was compared with AG and pediatric logistic organ dysfunction-2 (AG+PELOD-2). • The mortality assessment of pediatric septic patients showed that pSOFA was more sensitive than PELOD-2, while pSOFA+AG was not more sensitive than PELOD-2. Abstract: Sepsis and septic shock are some of the causes of morbidity and mortality (50-60%) in pediatric patients treated in intensive care rooms. This study aimed to compare the accuracy of pediatric Sequential Organ Failure Assessment (pSOFA) score combined with …

The Influence Of Safety Communications And Safety Promotion Policies On Safety Performance Among Nurses In The Emergency Department At A Tertiary Hospital In Surabaya, Indonesia, Ratih Berliana, Noeroel Widajati, Nurhayati Saridewi, Endang Dwiyanti

Folia Medica Indonesiana

Highlights: • To reduce the occurrence of work-related accidents, the application of occupational safety and health principles in work activities is necessary. • This research analyzed the safety communications and safety promotion policies on the safety performance among nurses. • Safety communications have influence on safety performance, while safety promotion policies have no influence on safety performance. Abstract: The application of the occupational safety and health principles in work activities is expected to reduce the occurrence of work-related accidents. This research was conducted in the Emergency Department of a tertiary hospital of Dr. Soetomo General Academic Hospital, Surabaya, Indonesia, in …

A Cross-Sectional Study Of The Prevalence Of Anal Dysplasia Among Women With High-Grade Cervical, Vaginal, And Vulvar Dysplasia Or Cancer: The Panda Study, Samantha Batman, Craig A. Messick, Andrea Milbourne, Ming Guo, Mark F. Munsell, Joel Fokom-Domgue, Mila Salcedo, Ashish Deshmukh, Kristina R. Dahlstrom, Mallory Ogburn

School of Medicine Publications

Background: High-risk human papillomavirus (HR-HPV) infection is a risk factor for anal cancer, yet no anal cancer screening guidelines exist for women with lower genital tract HPV-related disease. We sought to describe the prevalence of anal HR-HPV or cytologic abnormalities in such women.

Methods: This cross-sectional study was performed between October 2018 and December 2021. Inclusion criteria were ≥21 years of age and a prior diagnosis of high-grade dysplasia/cancer of the cervix, vagina, or vulva. Participants underwent anal cytology and anal/cervicovaginal HR-HPV testing. Women with abnormal anal cytology were referred for high-resolution anoscopy (HRA).

Results: 324 evaluable women were enrolled. …

A Cross-Sectional Study Of The Prevalence Of Anal Dysplasia Among Women With High-Grade Cervical, Vaginal, And Vulvar Dysplasia Or Cancer: The Panda Study, Samantha Batman, Craig A Messick, Andrea Milbourne, Ming Guo, Mark F Munsell, Joel Fokom-Domgue, Mila Salcedo, Ashish Deshmukh, Kristina R Dahlstrom, Mallory Ogburn, Anthony Price, Nicole D Fleming, Jolyn Taylor, Aaron Shafer, Lauren Cobb, Keith Sigel, Erich M Sturgis, Elizabeth Y Chiao, Kathleen M Schmeler

Faculty, Staff and Students Publications

BACKGROUND: High-risk human papillomavirus (HR-HPV) infection is a risk factor for anal cancer, yet no anal cancer screening guidelines exist for women with lower genital tract HPV-related disease. We sought to describe the prevalence of anal HR-HPV or cytologic abnormalities in such women.

METHODS: This cross-sectional study was performed between October 2018 and December 2021. Inclusion criteria were ≥21 years of age and a prior diagnosis of high-grade dysplasia/cancer of the cervix, vagina, or vulva. Participants underwent anal cytology and anal/cervicovaginal HR-HPV testing. Women with abnormal anal cytology were referred for high-resolution anoscopy (HRA).

RESULTS: 324 evaluable women were enrolled. …

How I Approach Leishmaniasis: Diagnosis And Treatment In The United States, Tara E Ness, Rachel Martin-Blais, Jill E Weatherhead

Faculty, Staff and Students Publications

Leishmaniasis is a vector-borne disease caused by over 20 species of obligate intracellular protozoa belonging to the genus Leishmania. Leishmaniasis has a global distribution, including in the United States, and can cause a spectrum of clinical syndromes, including cutaneous, mucosal, and visceral diseases depending on host factors and the infecting Leishmania spp. Accurate diagnosis, including Leishmania species identification, is an important step to guide the most appropriate therapeutic intervention. Antileishmanial therapy is dependent on the Leishmania spp. identified, the clinical syndrome, and the child's immune system. However, many treatment regimens for children have been extrapolated from adult clinical trials, which …

Impact Of International Travel And Diarrhea On Gut Microbiome And Resistome Dynamics, Manish Boolchandani, Kevin S Blake, Drake H Tilley, Miguel M Cabada, Drew J Schwartz, Sanket Patel, Maria Luisa Morales, Rina Meza, Giselle Soto, Sandra D Isidean, Chad K Porter, Mark P Simons, Gautam Dantas

2020-Current year OA Pubs

International travel contributes to the global spread of antimicrobial resistance. Travelers' diarrhea exacerbates the risk of acquiring multidrug-resistant organisms and can lead to persistent gastrointestinal disturbance post-travel. However, little is known about the impact of diarrhea on travelers' gut microbiomes, and the dynamics of these changes throughout travel. Here, we assembled a cohort of 159 international students visiting the Andean city of Cusco, Peru and applied next-generation sequencing techniques to 718 longitudinally-collected stool samples. We find that gut microbiome composition changed significantly throughout travel, but taxonomic diversity remained stable. However, diarrhea disrupted this stability and resulted in an increased abundance …

Quantitative Measures Used In Empirical Evaluations Of Mental Health Policy Implementation: A Systematic Review, Meagan Pilar, Eliot Jost, Callie Walsh-Bailey, Byron J Powell, Stephanie Mazzucca, Amy Eyler, Jonathan Purtle, Peg Allen, Ross C Brownson

2020-Current year OA Pubs

BACKGROUND: Mental health is a critical component of wellness. Public policies present an opportunity for large-scale mental health impact, but policy implementation is complex and can vary significantly across contexts, making it crucial to evaluate implementation. The objective of this study was to (1) identify quantitative measurement tools used to evaluate the implementation of public mental health policies; (2) describe implementation determinants and outcomes assessed in the measures; and (3) assess the pragmatic and psychometric quality of identified measures.

METHOD: Guided by the Consolidated Framework for Implementation Research, Policy Implementation Determinants Framework, and Implementation Outcomes Framework, we conducted a systematic …

Clark/Floyd Crisis Intervention Team Capstone Project, Troy L. Mansfield

Social Work Masters Capstone Projects

No abstract provided.

Salus Populi: Educating Judges On The Social Determinants Of Health, Faith Khalik, Alisa Lincoln

Journal of Legal Education

No abstract provided.

An Integrative Review: The Experiences Of Stress, Burnout, And Compassion Fatigue By Ambulatory Care Nurses, Rajean Adams

Master of Science in Nursing Final Projects

Abstract

Aim: One aim of this integrative review is to highlight workplace issues outpatient (ambulatory care) nurses experience, related to stress, burnout, and compassion fatigue. Another aim is to bring awareness to and discuss recently implemented tools and strategies to help alleviate these manifestations.

Background: Stressors in the outpatient setting date back to the 1970s when psychologist Herbert Freudenberger mentioned the burnout he experienced while in a Free Clinic. In modern-day, half of nurses experiencing workplace burnout, confess to feeling emotional exhaustion. Various studies discuss the challenges nurses endure in the inpatient setting, but only a few focus on ambulatory …

Medical School Watercooler Newsletter - December 2, 2022, Lindsey Lyle

Watercooler Newsletter

This is the December 2, 2022 edition of the Frederick P. Whiddon College of Medicine's newsletter - Watercooler.

Contents include:

• USA mourns the loss of Johnson Haynes Jr., M.D.
• Charles M. Baugh Lectureship set for Dec. 8
• Pathology research seminar to focus on community-based cancer screenings
• Whiddon College of Medicine faculty recognized as 'Top Profs'
• Study identifies potential biomarkers for earlier detection of pancreatic cancer

Does The Impact Of Arthritis On Participation In Social Activity Vary By Depression?, Mehnaaz Ali

Master of Public Health Program Student Publications

Objective As the population’s longevity is increasing over time, the number of comorbidities that individuals are suffering with are also becoming evident. The objective is to determine whether individuals living with arthritis have difficulty in social activity participation while suffering with depression. Using the 2019 National Health Interview Survey, this analysis included males and females between 18 to 85 years of age. The dependent variable was social activity participation, where it was identifying participants whether they are or are not having difficulty taking part in social activities. The primary independent variables were depression and arthritis. Other covariates included the demographics …

Antimicrobial Efficacy Of A Citric Acid/Hydrochloric Acid Blend, Peroxyacetic Acid, And Sulfuric Acid Against Salmonella On Inoculated Non-Conventional Raw Chicken Products, Emma Nakimera

Department of Food Science and Technology: Dissertations, Theses, and Student Research

The utilization of non-conventional chicken parts as human food varies widely across countries. The high prevalence of Salmonella, especially in the giblets, poses a high risk to public health. Poultry processors have implemented multiple hurdle technology to reduce this gram-negative pathogen in chicken parts. This study was conducted to evaluate the antimicrobial efficacy of a commercial blend of citric acid/ hydrochloric acid (CP), peroxyacetic acid (PAA), and sulfuric acid (SA) in reducing Salmonella inoculated on two chicken giblets: livers and hearts. Chicken hearts and livers were inoculated (6 log CFU/ml of rinsate) by individually immersing them in a cocktail …

December 2, 2022 Ereporter, University Of Alabama At Birmingham

eReporter (campus newsletter)

No abstract provided.

Descriptive Dna Barcoding Of Argas (Persicargas) Arboreus And Argas (Persicargas) Persicus Ticks (Ixodida: Argasidae) Infesting Birds In Egypt, Enas H. Ghallab, Ayat Yousery, Mona G. Shaalan

Harold W. Manter Laboratory of Parasitology: Library Materials

Argas ticks are primary parasites of birds with veterinary importance. Nevertheless, these ticks have received little attention regarding molecular identification studies. DNA barcoding is a powerful technique for identifying tick species besides traditional morphological identification. The present work is a first effort to divulge DNA sequences of Argas (Persicargas) arboreus from Egypt and worldwide. We used cytochrome c oxidase subunit I (COI) from A. arboreus infesting herons, and from the fowl tick Argas (Persicargas) persicus. Our results pointed out another success for the Folmer primers that are widely used in DNA barcoding, permitting the discrimination …

Addressing The Stigma Associated With Antiquated Blood Donation Policies And Donor Eligibility For Msm, Jay S. Pickern, Helena R. Costakis

Journal of the Georgia Public Health Association

The crises of blood product shortages pose real health risks and threats to the greater population in the United States (U.S.). Current U.S. blood donation policies continue to restrict or limit donations from willing and healthy individuals on the basis of sex, classified as men who have sex with men (MSM). This essay explores the historical progression of the current blood donation policy through the lens of enacted stigmatization and stigma consciousness for MSM, identifies implications of the current policy, explores the impact of the current policy and proposed changes, and calls for action to modernize the current blood donation …

Isolation And Characterization Of Flavonoid Naringenin And Evaluation Of Cytotoxic And Biological Efficacy Of Water Lilly (Nymphaea Mexicana Zucc.), Shajrath Din, Saima Hamid, Aadil Yaseen, Ali Mohd Yatoo

Faculty and Student Publications

Despite its limited exploration, Nymphaea mexicana Zucc. can be beneficial if pharmacology, isolation, and biological evaluation are given attention. It is an aquatic species that belongs to the family Nymphaeaceae. The thrust area of the work was the extraction, isolation, and biological evaluation of different extracts of the N. mexicana Zucc. plant. The primary goal of this research was to assess the antimicrobial, antioxidant, and anticancer activities of the extracts and to isolate the target naringenin compound. Comparative FT IR analysis of different extracts of this plant revealed the presence of functional groups of plant secondary metabolites, including polyphenols, flavonoids, …