Digital Commons Network^™

Crispr/Cas9 Whole-Genome Screens Identify Genes Critical For Neutral Lipid Metabolism In Macrophages, Rifat Sultana

Electronic Theses and Dissertations

Lipid-laden macrophages contribute to atherosclerotic plaque formation in atherosclerosisrelated heart diseases. Thus, understanding formation and degradation of lipid would help identify therapeutic targets for the treatment of atherosclerosis. In the first chapter of this dissertation, I have explained the current understanding of cholesterol metabolism in macrophages and how mTOR could play a potential role in this process. Additionally, I have described how CRISPR/Cas9 whole-genome screens present an effective tool to identify genes regulating cholesterol metabolism including positive and negative regulators of lipid droplet formation and lipid droplet degradation. In Chapter 2, I have described the results of the CRISPR/Cas9 whole-genome …

Genome-Wide Association Mapping And Genomic Prediction For Enhancing Fhb Resistance In Hard Winter Wheat, Jinfeng Zhang

Electronic Theses and Dissertations

Wheat is one of the most important staple crops providing 20% of energy for 35% of the world population. Fusarium head blight (FHB), primarily caused by the fugal Fusarium graminearum Schwabe, is a damaging disease in wheat that affects global wheat production every year and causes food safety issues. The disease not only reduces the grain yield and quality but also produces mycotoxin in the diseased kernels making them unsuitable for human consumption or as livestock feeds. Breeding FHB resistant cultivar is the most effective and economical approach to managing the disease. This study combines genome-wide association study (GWAS) and …

Efficacy Of Nanomaterials And Biorationals On The Bacterial Leaf Streak Management In Wheat, Abraham Hangamaisho

Electronic Theses and Dissertations

Wheat (Triticum aestivum L.) is one of the most important cereal crops in the United States. Most wheat varieties are susceptible to bacterial leaf streak (BLS), a major disease caused by Xanthomonas translucens pv. undulosa (Xtpvu). BLS is challenging to manage since common chemicals do not provide adequate control. Nanomaterials and plant extracts have shown potential to provide a sustainable environmentally friendly control of animal and plant diseases. However, limited data are available on the efficacy of plant extracts and nanomaterials controlling BLS in wheat. The objective of this study was to evaluate the antibacterial activity of plant extracts and …

Genistein-Enriched Pig Gut Microbiota Library As A Potential Probiotic Consortium, Theresah Amponsah

Electronic Theses and Dissertations

Plant-based diets contain numerous flavonoid compounds that produce antibacterial effects and gut health improvement. Genistein is among the most abundant isoflavones present in a plant-based diet and can be found in high amounts in soy products (up to 1g Kg-1). We describe here a robust method to identify genistein tolerant and metabolizing bacteria in swine gut microbiota and to screen the strains that have antibacterial and immunestimulatory properties. Such strains could be developed as non-antibiotic alternatives to prevent enteric infections in pigs and improve gut immunity. To this end, a mini bioreactorbased system was used to enrich genistein metabolizing bacteria …

Design, Synthesis, Structure Optimization, Structure-Activity Relationship (Sar), And Biological Evaluation Of Novel And Potent Thiazolo[4,5-D]Pyrimidine Corticotropin Releasing Factor (Crf) Receptor Antagonists, Md Rabiul Islam

Electronic Theses and Dissertations

No abstract provided.

Disentangling The Complex Evolutionary History Of Invasive Russian Thistle: Phylogenetic Relationships, The Role Of Hybridization, And Public Engagement, Deva Raj Khanal

Electronic Theses and Dissertations

Invasive species are forces of environmental, economic, and social change with detrimental impacts on ecosystems, commerce, agriculture, and human health. However, we still have a poor understanding of the processes that could be driving invasive potential. Hybridization may contribute to the establishment and spread of invasive lineages, as it may enhance genetic variation and facilitate rapid adaptation. Focusing on Russian thistle (Salsola tragus s.l., family Amaranthaceae) and closely related species, my thesis aims to clarify phylogenetic relationships and investigate how hybridization has shaped the invasion dynamics of these lineages. Known as one of the fastest plant invasions in the United …

Prairie Cordgrass Cellulose: A Sustainable Resource To Develop Biodegradable Films, Mominul Hoque

Electronic Theses and Dissertations

Plastics are versatile and cost-effective materials with a wide variety of functionalities. Their non-biodegradability, however, is an environmental concern. Plastic waste accumulation in landfills and natural habitats results in health issues, mainly due to the leaching of chemicals from plastic products that further transfer to humans and wildlife. Many countries around the globe have imposed restrictions on plastic use. Despite these concerted efforts, a pressing scientific need exists to find an alternative to plastics. Cellulose from abundant agricultural biomass stands out as a viable alternative due to its robust structure, biocompatibility, biodegradability, low density, and non-toxicity. This research aims to …

A Post-Pneumonia Epizootic Evaluation Of The Rapid City, South Dakota Bighorn Sheep Herd, Amanda N. Ensrud

Electronic Theses and Dissertations

Pneumonia is a major factor affecting populations of free-ranging bighorn sheep (Ovis canadensis) across western North America. Pneumonia can occur in large-scale epizootics, during which greater than half of the population typically dies. After these epizootics, surviving ewes continue to conceive and bear lambs. However, lamb recruitment may remain low due to periodic or annual pneumonia outbreaks causing high lamb mortality rates, sometimes greater than 90%. Our study focused on the Rapid City, South Dakota bighorn sheep (BHS) herd that has recorded pneumonia-induced population decline since 2009. The first objective was to improve lamb health and survival by identifying and …

Northern Great Plains Saline Sodic Soil Development, Classification, Remediation, And Management., Shaina Westhoff

Electronic Theses and Dissertations

Soil salinity and sodicity are issues of growing concern in the United States (U.S.) and globally. Knowledge gaps for glaciated, dryland salt-affected soils exist because much original salinity and sodicity research focused on irrigated systems. Land managers are being asked to produce food, feed, fiber, and fuel for an expanding global population. The number of land managers and crop advisors who are affected by these soils is increasing. Addressing salinity and sodicity knowledge gaps will be critical for their success. Salinity and sodicity have been impeding crop productivity since the advent of cultivation. Saline and sodic soils form via multiple …

Covid-19, Churches, And Culture Wars, John D. Inazu

Scholarship@WashULaw

The First Amendment’s Free Exercise Clause often requires courts to balance competing interests of the highest order. On the one hand, the Constitution recognizes the free exercise of religion as a fundamental right. On the other hand, the government sometimes has compelling reasons for limiting free exercise, especially in situations involving dangers to health and safety. The shutdown and social distancing orders issued during the early phases of the COVID-19 pandemic not only restricted free exercise but also limited what many people consider to be the core of that exercise: religious worship. But the orders did so in order to …

Cost-Based California Effects, Jens Frankenreiter

Scholarship@WashULaw

The “California Effect” is a recurring trope in discussions about regulatory interdependence. This effect predicts that businesses active in multiple jurisdictions sometimes adopt the strictest standards that they face in any jurisdiction globally, even if the law does not require global compliance. There is a substantial literature that assumes the existence of California Effects both at the interstate level in the United States and the international level. However, empirical evidence documenting their existence and strength is scarce. This paper investigates the existence of California Effects in data privacy law, a field in which these effects have been said to be …

The Root And Branches Of Structural School Racism In Missouri: A Story Of Failure By Design And The Illusion And Hypocrisy Of School Choice, Kimberly Jade Norwood, Ronald Alan Norwood

Scholarship@WashULaw

Since Missouri was first admitted into the Union as a slave state, it has been hostile to the education of its Black residents. This article examines the evolution of that hostility from 1821 through 2021 (from the most overt and blatant in the early years, to the subtler and covert in the modern era). Starting with the original total ban on the education of Black slaves to the reluctant allowance of separate but equal education for Black Missouri residents in 1865 after the Civil War and continuing with the separate but unequal policies that have thrived in the state from …

The Role Of State Attorneys General In Improving Prescription Drug Affordability, Michelle M. Mello, Trish Riley, Rachel Sachs

Scholarship@WashULaw

Impact litigation initiated by state attorneys general has played an important role in advancing public health goals in contexts as diverse as tobacco control, opioids, and healthcare antitrust. State attorneys general also play a critical role in helping governors and legislatures advance health policies by giving input into their drafting and defending them against legal challenges. State attorneys general have entered the prescription drug affordability arena in both these ways—for example, by initiating lawsuits relating to price fixing by generic drug manufacturers and defending state laws requiring disclosures of pharmaceutical prices. Yet the scope of their collective efforts is not …

New Innovation Models In Medical Ai, W. Nicholson Price Ii, Rachel Sachs, Rebecca S. Eisenberg

Scholarship@WashULaw

In recent years, scientists and researchers have devoted considerable resources to developing medical artificial intelligence (AI) technologies. Many of these technologies—particularly those that resemble traditional medical devices in their functions—have received substantial attention in the legal and policy literature. But other types of novel AI technologies, such as those related to quality improvement and optimizing use of scarce facilities, have been largely absent from the discussion thus far. These AI innovations have the potential to shed light on important aspects of health innovation policy. First, these AI innovations interact less with the legal regimes that scholars traditionally conceive of as …

Judge Theodore Mcmillian: Beacon Of Hope And Champion For Justice, Karen L. Tokarz

Scholarship@WashULaw

“Judge Theodore McMillian: Beacon of Hope and Champion for Justice” illuminates the heroic groundbreaking accomplishments of Judge McMillian, who was a trailblazer in Missouri courts. Judge McMillian was Missouri’s first Black judge to sit on the state circuit court, state appellate court, and federal appellate court. Professor Tokarz traces Judge McMillian’s early life and career to demonstrate his life-long dedication to challenging disparities in the community and in the legal system. She discusses the Judge’s role on the St. Louis City Circuit Court, especially the Juvenile Court where he pushed for the expansion of constitutional rights for juveniles; his groundbreaking …

Optimizing Social Welfare In The Anthropocene, Max Bradley Sternberg

Student Scholarship

No abstract provided.

Asian/Americans: Model Students, Disempowered Citizens, Sueshin Sarah Moon

Student Scholarship

No abstract provided.

Stand Your Ground: Black Women And Perceived Gender Schema Trangessions, Chelsey Minerva Sarante

Student Scholarship

No abstract provided.

Female Domestic Literary Horror Through The Lens Of The Contemporary Work Of Carmen Maria Machado, Cassidy Crane

Student Scholarship

No abstract provided.

Kelly Brown Douglas: Womansim & Intersectional Theology, Rachel Ellen Cryberg

Student Scholarship

No abstract provided.

Kindness In Black And Latino Households, Mia Camille Caraballo-Collazo

Student Scholarship

No abstract provided.

Let Justice Be Done: Cancel Culture And Demanding Social Changes In Vietnam, Ha Thi Thanh Doan

Student Scholarship

No abstract provided.

Criminal Justice Expertise, Benjamin Levin

Scholarship@WashULaw

For decades, commentators have adopted a story of mass incarceration’s rise as caused by “punitive populism.” Growing prison populations, expanding criminal codes, and raced and classed disparities in enforcement result from “pathological politics”: voters and politicians act in a vicious feedback loop, driving more criminal law and punishment. The criminal system’s problems are political. But how should society solve these political problems? Scholars often identify two kinds of approaches: (1) the technocratic, which seeks to wrest power from irrational and punitive voters, replacing electoral politics with agencies and commissions; and (2) the democratic, which treats criminal policy as insufficiently responsive …

Intraoperative Post Partum Hemorrhage In A Patient With Dengue Fever, Usama Ahmed, Asiyah Aman

Department of Anaesthesia

A 33 year old obstetric patient with mild fever of undiagnosed etiology underwent emergency caesarean section under general anesthesia. She had platelet count of 98,000 per microliter and increased APTT of 37.8 s at the time of surgery. After uneventful anesthetic induction and delivery of fetus, slow and oozing type of bleeding led to massive hemorrhage. Patient remained vitally stable throughout perioperative phase and was extubated. Next day, patient's dengue IgM antibody was reported positive. Neonate was well and his dengue test was negative. Pregnant women are at high risk of developing severe complications of dengue fever with unclear mechanisms …

Pet Imaging Reveals Reduced Synaptic Density In People With Well-Controlled Hiv Infection, Julian Joseph Weiss

Yale Medicine Thesis Digital Library

Background: Synaptic injury is a pathological hallmark of neurological impairment in people living with HIV (PLWH), a common complication despite viral suppression with antiretroviral therapy (ART). However, there are no biomarkers sensitive to neurocognitive impairment in PLWH. Measurement of synaptic density in living humans may allow better understanding of HIV neuropathogenesis and provide a dynamic biomarker for therapeutic studies. We applied novel synaptic vesical protein 2A (SV2A) positron emission tomographic (PET) imaging to investigate synaptic density in the frontostriatalthalamic region in PLWH and HIV-uninfected (HIV-) participants.

Methods: In this cross-sectional pilot study,13 older male PLWH on ART underwent neurocognitive assessments, …

"How To Succeed": Determining And Comparing The Musical And Non-Musical Influences Behind The Broadway Adaptation Of How To Succeed In Business Without Really Trying, Gary Bogers

Electronic Theses and Dissertations, 2020-2023

The following research project is the result of comparing and contextualizing the original non-musical and musical drafts of the original 1961 Broadway production of How to Succeed in Business without Really Trying. The intention is to detail the process of musically adapting a non-musical source, specifically the 1952 satirical text How to Succeed in Business without Really Trying: The Dastard's Guide to Fame and Fortune by businessman-turned-satirist Shepherd Mead. Research was predominantly completed through analysis of the non-musical draft written by television writers Jack Weinstock and Willie Gilbert in the 1950s and acquired from the Billy Rose Theatre Division of …

Breaking The Mold: Haunted Attraction Mazes; A Study In Reducing Predictability & Increasing Intensity, Within Unconventional Halloween Attraction Experiences, Amy Avalos

Electronic Theses and Dissertations, 2020-2023

Every enchanted Fall season, a segment of the public flocks to a multitude of haunted Halloween attractions across the U.S., to experience fear as a form of amusement and entertainment. Psychologists continue to research the physiological and cognitive behaviors that are associated with fear and the "fight or flight" responses that are triggered when individuals are engaged in a heighten sense of danger. However, as guests traverse through these haunted attraction experiences, it's commonplace for guests to enter into these typical modes of "fight or flight" behaviors very quickly. Once this response kicks in, the guests' instinct is to scramble …

Choreographing Closeness: The Effects Of Intimacy Choreography Best Practices In Educational Theatre, Morgan Cobb

Electronic Theses and Dissertations, 2020-2023

Power dynamics between teacher/director and student/actors in an educational theatre setting are inevitable and directly impact the way consent is viewed and given, specifically with youth performers. In response to the #MeToo movement within theatre and film, the role of intimacy choreographer emerged with the goal of creating a consent-based rehearsal process that ensured the mental, emotional, and physical safety of the actor (Pace 25) while conveying the intimacy required to effectively support the script. The use of intimacy choreographers is almost exclusively used in professional film and theatre settings. Applying the pedagogies created and taught by leading intimacy organizations …

Nurse Managers' Patient Safety Communication, Christine Deatrick

Electronic Theses and Dissertations, 2020-2023

Medical errors are a continuing issue for healthcare organizations and can lead to patient harm or death. To bring about organizational learning and therefore reduce medical errors, information must successfully flow through an organization. Nurse managers play an essential role as the conduit of information between frontline staff and the organization. The primary purpose of this study is to describe the decision-making process of how nurse managers actively select and transmit information on patient safety concerns. A qualitative constructivist grounded theory approach was used. Nineteen unit-level nurse managers, working full-time in an acute care hospital with a minimum of one-year …

For The Very First Time: A Process Of Rehearsal And Performance In The Beginnings Of Covid-19., Andrew Hansen

Electronic Theses and Dissertations, 2020-2023

Acting is a discipline that changes over time not only for the audience, but for the actors who create it. Not only is their craft shaped through study, practice, and repetition that we as individuals have towards the art form, but it is strongly influenced by our environment and personal histories. As actors, we have to bear in mind what we are presenting, when we are presenting it, and why. These choices are a collaborative effort made through the discussion of various artists including, actor, director, writer, etc. For my thesis, I would like to show you what my journey …