Multiplex Immunofluorescence-Guided Laser Capture Microdissection For Spatial Transcriptomics Of Metastatic Melanoma Tissues., Jan Martinek, Te-Chia Wu, Lili Sun, Jianan Lin, Kyung In Kim, Florentina Marches, Paul Robson, Joshy George, Karolina Palucka

Faculty Research 2022

We describe a pipeline for optimized and streamlined multiplexed immunofluorescence-guided laser capture microdissection allowing the harvest of individual cells based on their phenotype and tissue localization for transcriptomic analysis with next-generation RNA sequencing. Here, we analyze transcriptomes of CD3+ T cells, CD14+ monocytes/macrophages, and melanoma cells in non-dissociated metastatic melanoma tissue. While this protocol is described for melanoma tissues, we successfully applied it to human tonsil, skin, and breast cancer tissues as well as mouse lung tissues. For complete details on the use and execution of this protocol, please refer to Martinek et al. (2022).

Extrachromosomal Dna (Ecdna): An Origin Of Tumor Heterogeneity, Genomic Remodeling, And Drug Resistance., Lauren T Pecorino, Roel G W Verhaak, Anton Henssen, Paul S Mischel

Faculty Research 2022

The genome of cancer cells contains circular extrachromosomal DNA (ecDNA) elements not found in normal cells. Analysis of clinical samples reveal they are common in most cancers and their presence indicates poor prognosis. They often contain enhancers and driver oncogenes that are highly expressed. The circular ecDNA topology leads to an open chromatin conformation and generates new gene regulatory interactions, including with distal enhancers. The absence of centromeres leads to random distribution of ecDNAs during cell division and genes encoded on them are transmitted in a non-mendelian manner. ecDNA can integrate into and exit from chromosomal DNA. The numbers of …

Frederick Smith, Jr. V. Springhill Suites

2022 Decisions

USDC for the District of Delaware

Jonathan Gould V. Robert O\'Neal

2022 Decisions

USDC for the District of New Jersey

Vietnam Places Market Logic Above Workers’ Rights, Angie Ngọc Trần

SSGS Faculty Publications and Presentations

No abstract provided.

Contributions Of Capitals To Chinese International Graduates’ Employability In Australia

Journal of Comparative & International Higher Education

No abstract provided.

Transcranial Direct Current Stimulation Of Frontal/Occipital Cortices Modulates Neural Correlates Of Cognition In Healthy Young Adults, Yasra Arif

Theses & Dissertations

An exponential rise in the use of transcranial direct current stimulation (tDCS) to modify the human brain and behavior has been observed over the last 20 years, with studies often yielding mixed outcomes. Such heterogeneity is partially attributed to the lack of a concise understanding of the underlying mechanisms. Though considered to exert modulatory effects by changing neuronal membrane polarization and synaptic efficacy, the interactive effects of offline tDCS and the neural underpinnings of cognition, both at the regional and network-level, are yet to be fully understood. Using High-Definition tDCS (HD-tDCS), Magnetoencephalography (MEG), a range of well-established cognitive paradigms, and …

Culture Care Values And Beliefs Of Puerto Rican Adults Related To Suicide: An Ethnonursing Study, Griselle Estrada

Electronic Theses and Dissertations

BACKGROUND: Suicide is a public health issue among the growing U.S. Latinx population; however, Latinx subgroups are diverse and represent different cultural backgrounds. Puerto Ricans are the second largest Latinx subgroup in the nation. Although research points to possible mental health vulnerabilities among Puerto Ricans when compared to other Latinx subgroups, there is a dearth of research exploring cultural influences that can impact suicide prevention efforts for Puerto Ricans living in the U.S. mainland. AIMS: This study aimed to explore the culture care values, beliefs, and needs of Puerto Rican adults pertaining to suicide and its prevention. METHODS: The …

An Assessment Of Improvement Challenges & Lessons Learned From Feeding Students Of A Large Urban School District During The Covid-19 Pandemic, Jamar King

Electronic Theses and Dissertations

This qualitative dissertation study took place in the midst of the COVID-19 pandemic. The purpose of this study was to examine the work of school food service professionals as this has implications for all school food service staff. Specifically, this study focused on the role of the cafeteria managers, through their practices, beliefs, the support needed, and challenges they faced during a complicated reality of meeting student needs, and to contribute to the improvement practices in school food service for future emergencies. The research questions, methods, and data analysis are framed using the first three principles of Improvement Science described …

Adaptive Microlearning: An Empirical Study Among The Students In The Rural Areas Of China, Jingwei Liu

Electronic Theses and Dissertations

Through multiple educational reforms, China has pursued developing well-rounded learners with solid content knowledge, higher-order thinking skills, and the ability to transfer knowledge to real-world problem spaces. The imbalance of learning resources between urban and rural China, however, has caused disadvantages for learners in rural China to gain access to quality educational opportunities necessary for becoming well-rounded learners. Luckily, the quickly growing Internet service coverage in rural China makes educational interventions possible. Online intervention aiming for many qualities of well-rounded learners, such as deeper learning, is particularly promising.

The purpose of this study was to investigate the potential of adaptive …

Assessments Of Water Chemistry And Fish Community Structure In The Buffalo Creek Watershed, Tributary To The Allegheny River In Armstrong And Butler Counties, Pennsylvania, Kathleen Wilson

Electronic Theses and Dissertations

The Buffalo Creek watershed has experienced water quality impairments for various reasons, including but not limited to, agricultural influence, abandoned mine drainage (AMD), on-site wastewater malfunction, and stormwater. The source and cause of the most prevalent impaired areas remains unknown. The goal of this study was to analyze water chemistry using state water quality standards and mass ratio analysis, then to conduct fish community analysis. We found toxic metal pollution across the watershed, with iron, cadmium, copper, and selenium frequently exceeding criteria. We determined which streams are polluted by resource extraction activities by conducting mass ratio analysis. We found conventional …

The Usability And Feasibility Of A Nursing Evidence-Based Comorbidity Educational Website For An Urban Mental Health Assertive Community Treatment (Act) Program, Andrea Brudigan, Laurie Sewell-Muller, Kokuvi Sena Tsogbevi

Doctor of Nursing Practice Projects: College of Nursing

Objective: Serious mental illness (SMI) causes an increased risk for comorbidities like heart disease, obesity, metabolic syndrome, and diabetes. Many times, these comorbidities are due to side effects of antipsychotics and poor self-management from low insight and other symptoms of mental illness. This project aims to enhance client care enrolled in an Assertive Community Treatment (ACT) program by providing evidence-based resources tailored to clients with SMI, providing ACT team members with support to promote healthy behaviors in clients.

Methods: A website was developed that provides printable, easy-to-read, evidence-based resources that address physical comorbidities. The ACT team integrated these resources into …

2022-12-16_Michiganoverview_Covidbedppe, Michigan Department Of Health & Human Services

Michigan COVID-19 Hospital Data

Contains the tables Statewide Hospital Capacity Report for COVID-19, COVID-19 Metrics, Statewide Hospital Available PPE Tracking for COVID-19, as well as the tables PPE Days On Hand and Patient Census.

Open Educational Resources Adoption Reduces Textbook Costs Without Sacrificing Student Performance In Business And Economics Courses At A Community College, Dorina Tila, Dawn Levy

Publications and Research

This study analyzed the possibility of saving on textbook costs without sacrificing student performance by using Open Educational Resources (OER) at no cost to students as a replacement for high-priced commercially printed books. Approximately two hundred students enrolled in business and economics courses at Kingsborough Community College of the City University of New York from Fall 2016 to Fall 2018 were assessed in this study. Using summative assessments and course grades as a measure of students’ performance, the findings showed that students in courses using OER as compared to students in the same subject area using traditional textbooks perform equally …

Possible Overestimation Of Nitrogen Dioxide Outgassing During The Beirut 2020 Explosion, Ashraf Farahat, Nayla El-Kork, Ramesh P. Singh, Feng Jing

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

On 4 August 2020, a strong explosion occurred near the Beirut seaport, Lebanon and killed more than 200 people and damaged numerous buildings in the vicinity. As Amonium Nitrate (AN) caused the explosion, many studies claimed the release of large amounts of NO₂ in the atmosphere may have resulted in a health hazard in Beirut and the vicinity. In order to reasonably evaluate the significance of NO₂ amounts released in the atmosphere, it is important to investigate the spatio-temporal distribution of NO₂ during and after the blast and compare it to the average day-to-day background emissions from …

Should I Stay Or Should I Go: The Impact Of Crossing Migrants In Local Communities In Mexico, Norma M. De La Rosa-Bustamante

Whittier Scholars Program

The interactions between migrants and Mexican local communities have positive and negative outcomes. A report by Human Rights First found that more than 630 violent crimes against asylum seekers were reported in the first few months of the “Remain in Mexico” policy. Still, some migrants have been able to assimilate and stay in Mexico, particularly in large cities such as Tijuana, Baja California and Ciudad Juarez, Chihuahua. This research project combines qualitative data collected through interviews with local NGOs between September 2020 to February 2021 and secondary research data. It focuses on the living conditions of migrants who have stayed …

2022 In Review: A Banner Blazer Year, Reporter Staff

Browse All News

No abstract provided.

2022 In Review: Uab's Gold-Medal Performance, Reporter Staff

Browse All News

No abstract provided.

Mitigation Impact Of Statewide Non-Pharmaceutical Policies On Covid-19: An Application Of Infectious Disease Transmission Model And Partially Observed Markov Process To New Mexico, Xingya Ma

Mathematics & Statistics ETDs

This thesis is an application of epidemiological models for infectious disease transmission and the use of partially observed Markov process (POMP) for model fitting. It focuses on COVID-19 pandemic in the state of New Mexico. The analysis covered March 2020 to June 2021. Daily data of COVID19 cases and deaths and a daily index of eleven statewide government non-pharmaceutical intervention (NPI) policies were collected from six public sources and were validated. These data were integrated through the Susceptible-Exposed-Infected-Removed (SEIR) model. Estimated daily transmission rates between the model compartments quantify the impact of the mitigation policies, and show that transmission rates …

Delineating Differences In How Us High Schools Are Racialized, Dara Shifrer, C. J. Appleton

Sociology Faculty Publications and Presentations

Schools’ overt or explicit practices are a dominant lens through which education researchers and policymakers attempt to understand how schools are racially inequitable. Yet, Lewis and Diamond argue that contemporary racial inequalities are largely sustained through implicit factors, like institutional practices and structural inequalities. Ray’s framework on racialized organizations similarly outlines how our racialized sociopolitical structure becomes embedded in organizations, legitimating and perpetuating the racialized hierarchy. We apply illustrative cluster analysis techniques to rich data on schools, teachers, and students from the nationally representative High School Longitudinal Study of 2009 to find that structural inequities (e.g., student body, sector, average …

Diabetes Prevention And Management Education For Punjabi-Sikh Older Adults, Mandeep K. Gill

Doctor of Nursing Practice (DNP) Projects

Abstract

Background: South Asians from Bangladesh, Pakistan, and India have a higher prevalence of diabetes than the general global population and are also more susceptible to developing diabetes due to biological and lifestyle factors. The South Asian diet is high in refined grains and monounsaturated fatty acids, increasing the risk of diabetes.

Local Problem: Congregants of a Sikh gurdwara (place of worship) lacked knowledge of diet and lifestyle in relation to preventing and managing diabetes. Many senior congregants have been diagnosed with diabetes or are at high risk of developing the disease.

Methods: Knowledge of diabetes prevention and management were …

Oleoresin Capsicum Gel: Enhancing Safety For The Home Health Clinician, Lilly Dickens

Doctor of Nursing Practice (DNP) Projects

Problem: Home health clinicians (HHC) are at high risk for workplace violence. Providing necessary health care alone in unpredictable and remote environments are associated with poor outcomes from physical violence. Background: Pepper spray is known for its active ingredient, oleoresin capsicum (OC), as a self-defense tool. OC gel is used worldwide as a non-lethal incapacitating agent. There is an opportunity to expand workplace violence prevention (WPV) in home health by training and allowing HHCs to carry OC gel. Intervention: This DNP QI project implemented a safety intervention for HHCs, including nurses, aides, medical social workers, physical therapists, occupational therapists, and …

Capstone Portfolio: Four Health Stories, Starring Unruly Uteri, Julie S. Schneider

Capstones

This capstone portfolio by Julie Schneider features four health stories, each starring unruly uteri. Articles include a feature story about hysterectomy and grief, an explainer about what happens to a uterus after a hysterectomy, a deep dive into funding for “femtech,” and a look at the potential impact that updated guidelines might have on endometriosis patients.

Link to capstone project: http://julie-schneider-capstone.tilda.ws/
(username = capstone & password = capstone)

Human Composting Is Changing The Landscape Of Death Care (Literally) — And It Could Be Legal In New York State As Soon As Next Year, Rachael Robertson

Capstones

Soon New Yorkers might have a new option for end of life care: Natural Organic Reduction, or human composting. Rather than being buried or cremated, New Yorkres might have the option of having their body composted and turned into soil as an environmentally friendly burial alternative. A bill legalizing Natural Organic Reduction in NYS and setting regulations similar to cremation is sitting on Governor Hochul's desk. Death, remembrance, and disposition are especially difficult in New York City, where there is not enough cemetery space for the number of people who die annually. Through audio, data, and print, I explore how …

Death Doulas And The Changing Face Of Passing On In America, Uvie M. Bikomo

Capstones

This project examines the damages of the current death culture in the country and the ways in which it is evolving to a more communal thing. It focuses on death doulas and how they are changing death in America.

Link to capstone project:https://ubikomo.github.io/doula/

Football Can Save America, Francisco Attie

Capstones

Looking to cure the malaise of our day, I went to the football game, and, in the dying breaths of the match, found something much greater. https://medium.com/@Fran_Attie/football-can-save-america-2bb918f19a97

To Destroy Or Transform? Two Fossil Fuel Transitions Offer Glimpse Into Industry’S Future, Chloe Bennett, Sarah Kerson

Capstones

In 2019, the Philadelphia Energy Solutions oil refinery closed after a dangerous explosion. But for years before the accident, members of the local environmental justice organization Philly Thrive had been advocating for its closure. Neighborhood residents who live near the refinery site had been complaining for years of health problems, ranging from asthma to cancer. “Enough is enough,” says Sylvia Bennett, 79, who lives in the Grays Ferry neighborhood near where the refinery is located. Her two daughters have both been diagnosed with cancer.

What will become of the refinery site remains to be seen as conversations between Hilco and …

Report Card: Nyc's Student-Powered Newsroom, Julian Roberts-Grmela

Capstones

During my time in the engagement journalism program at CUNY, I aimed to use journalism to serve the community of students in New York City’s public school system. At first, I tried to serve students through my reporting, by aiming to center student perspectives in education-related stories in order to uplift their feedback about the system. But I realized I could do more to report with students, instead of just about them. So, during my final semester, I launched Report Card: NYC’s Student-Powered Newsroom. Report Card is a Substack-based newsletter and a training program for middle-high school aged students …

Decreasing Intent To Leave In Nurse Residents (Nr) With The Addition Of Wellness Interventions: A Quality Improvement Project, Susan Eckman

Doctor of Nursing Practice (DNP) Manuscripts

The US nursing workforce decreased 1.8 percent between 2019 and 2021, marking the largest drop seen in four decades. New nurses or nurse residents NRs) are included in this loss. Many nurses, but particularly new nurses, experience burnout. If workplace stress is not managed, it can become chronic. The first year of employment as a nurse has been shown to be a very vulnerable time. Within the first 2 years of nurses’ employment, there is a reduction in commitment along with increased stress. New graduate nurses’ lack of life experiences, along with the recent pandemic have contributed to more than …

A Process Evaluation On The Health Care Transition Process For Youth With Osteogenesis Imperfecta With Special Health Care Needs, Janet Amirkhanian

Doctor of Nursing Practice (DNP) Manuscripts

With medical, surgical, and technological advances, children with childhood diseases with special health care needs (SHCNs) are surviving and living into adulthood (Cohen et al., 2014). Health care transition (HCT) should be a process through which youth are expected to achieve the skills necessary to manage their health as adults (Sawicki et al., 2017). HCT should be a purposeful, organized, and coordinated process involving the youth, family, and health care team (Stringer et al., 2015; Gabriel et al., 2017; White et al., 2018). Evidence supports that adolescents and young adults with SHCNs are often unprepared for transition (Jensen et al., …