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The Enemy Within: An Investigation Of The Intracellular Bacteria In Urinary Tract Infections, Jennie Elizabeth Hazen

Arts & Sciences Graduate Student Theses and Dissertations

Urinary tract infections (UTIs) are common diseases that are associated with significant morbidities. Multiple studies have indicated that multiple species of uropathogenesis bacteria invade and persist within bladder epithelial cells as a necessary step of uropathogenesis. Interestingly, many of these species are not canonically associated with intracellular infections. Although the first study describing bacteria within the urothelium was published two decades ago, this critical step of uropathogenesis remains relatively understudied.I established a murine model of community-acquired A. baumannii UTI, a previously unstudied manifestation of the disease. While immunocompetent mice resolved their infections quickly, immunocompromised mice displayed high bacterial burdens throughout …

A Universal Mechanism Of G Protein Inhibition, Tyson Daniel Todd

Arts & Sciences Graduate Student Theses and Dissertations

G protein coupled receptors transduce a truly staggering number of diverse extracellular signals including chemical messengers, physical force, and even photons into specific cellular responses through their coupling to heterotrimeric G proteins. G proteins amplify the originating signal through their binding to downstream effectors, activating a complex network of overlapping responses that allow the cell to respond perfectly to that specific stimulus. It is critical to the cell that this process is carried out faithfully in order to respond to the myriad environmental cues and avoid injury, exhaustion, and death for the individual cell or the development of pathology if …

Non-Canonical Beta-Lactam Antibiotics, Brett Wesley Virgin-Downey

Arts & Sciences Graduate Student Theses and Dissertations

In canonical β-Lactam antibiotics, such as penicillin, the strained nature of the β-Lactam ring leads to acylation of the catalytic serine in the active site of transpeptidases. In 2018, our lab reported a novel mechanism for monocyclic β-lactam antibiotics in the inhibition of glutamine synthetase (GS) by a natural product, tabtoxinine-β-lactam (TβL) using a 3-hydroxy-β-lactam pharmacophore. This work aims to generalize the β-lactam scaffold from TβL to act as a warhead against ATP-dependent carboxylate-amine ligase enzymes, with dihydrofolate synthetase (DHFS) as the test case. A pure 3-hydroxy-β-lactam warhead was synthesized and ligated both chemically and chemoenzymatically to different pteridine mimics. …

Dysregulation Of Gene Expression In Non-Photosensitive Trichothiodystrophy, Brittany Townley

Arts & Sciences Graduate Student Theses and Dissertations

The pre-mRNA life cycle requires intron processing; yet, how intron processing defects influence splicing and gene expression is unclear. Here, we find TTDN1, which is frequently mutated in non-photosensitive trichothiodystrophy (NP-TTD), functionally links intron lariat processing to the spliceosome. The conserved TTDN1 C-terminal region directly binds lariat debranching enzyme DBR1, while its N-terminal intrinsically disordered region (IDR) binds the intron binding complex (IBC). The IDR forms condensates in vitro and is needed for IBC interaction. TTDN1 loss causes significant intron lariat accumulation, as well as splicing and gene expression defects, mirroring phenotypes observed in NP-TTD patient cells. Ttdn1∆/∆ mice recapitulate …

Tracking Reducing Equivalents Between Cellular Compartments To Understand The Metabolic Demands Of Proliferation, Yahui Wang

Arts & Sciences Graduate Student Theses and Dissertations

Proliferating cancer cells compartmentalize metabolic reactions, many of which require reducing equivalents. This compartmentalization is functionally important, but it is challenging to study with current technologies. As a consequence, our understanding of proliferating metabolism has been limited. Here, I developed an approach to track reducing equivalents between cellular compartments. By applying this method to a panel of different cell lines under various conditions, I investigated a dysregulated metabolic phenotype in proliferating cells known as “aerobic glycolysis”. Aerobic glycolysis is characterized by an elevated rate of glucose fermentation to lactate irrespective of oxygen availability. Using the NCI-60 cell lines, I first …

Understanding Gene Regulation With High-Throughput Genome-Integrated Reporter Assays, Clarice Hong

Arts & Sciences Graduate Student Theses and Dissertations

Proper spatiotemporal gene expression depends on specific interactions between cis-regulatory sequences (CRSs) and chromatin modifications at a genomic location. However, we do not yet understand the rules that determine how CRSs work together to influence gene expression. The goal of this thesis is to systematically assay and quantify how different genomic environments interact with CRSs to control gene expression output using a variety of genome-integrated massively parallel reporter assays (MPRAs). First, we tested the hypothesis that core promoters confer specificity by only responding to compatible enhancers and chromatin in specific genomic environments. We measured the activities of hundreds of core …

The Effect Of Ionization Density In Applications Of Radiation Detection, Dosimetry, And Therapy, Daniel Mulrow

Arts & Sciences Graduate Student Theses and Dissertations

This dissertation covers a wide range of topics but is linked by the common theme of radiation interacting with materials and studying the result of those interactions. The introduction describes the fundamentals of how radiation interacts with material and how we are able to detect that radiation and the application of how we use those interactions in radiation oncology. The thesis starts with a chapter detailing the temperature dependence of the photophysics in two organic scintillators. This chapter is the foundation for a future study that will look the degree to which these scintillators can distinguish between gammas and neutrons …

Interpersonal Emotion Regulation In Current And Remitted Major Depressive Disorder: An Experience Sampling Study, Yunjing Liu

Arts & Sciences Graduate Student Theses and Dissertations

Individuals with major depressive disorder (MDD) have difficulties regulating emotion on their own. As people also turn to others for help with emotion regulation (i.e., interpersonal emotion regulation [IER]), we examined whether these difficulties extend to IER in current and remitted MDD. We generally expected individuals with current MDD (and remitted MDD to a lesser extent) to utilize IER in distinct ways compared to those with no history of psychiatric disorders (i.e., controls) due to differences in emotional, cognitive, and interpersonal functioning. Using experience sampling, adults with current MDD (n=48), remitted MDD (n=80), and controls (n=87) reported on how frequently …

Systems Level Analysis Of Organelle Biogenesis In Saccharomyces Cerevisiae, Kiandokht Panjtan Amiri

Arts & Sciences Graduate Student Theses and Dissertations

Eukaryotic cells contain hundreds of subcellular structures that serve different functions to maintain cellular homeostasis. A hallmark of Eukaryotic cells is its compartmentalization into membrane-bound organelles. One of the grand challenges in quantitative cell biology is understanding the precision with which cells assemble and maintain subcellular organelles. Despite identification of numerous molecular factors that regulate organelle sizes we lack insight into the quantitative principles underlying organelle size control. We examine organelle sizes from Saccharomyces cerevisiae and human iPS cells with mathematical theory to show that cells can robustly control average fluctuations in organelle size. By demonstrating that organelle sizes obey …

The Effects Of Nmda Antagonism On Neuronal Activity And Neurovascular Coupling In Non-Human Primate Cortex, Benjamin Thomas Acland

Arts & Sciences Graduate Student Theses and Dissertations

The N-methyl-D-aspartate receptor (NMDAR) plays a variety of important roles in the development and function of primate central nervous systems. This thesis describes three nonhuman primate studies that, together, demonstrate that pharmacologically reducing NMDAR activity not only causes dramatic changes in neural activity in cortex, but also changes the relationship between that activity and a key signal often used as its proxy in human studies, the blood-oxygen-level-dependent (BOLD) signal. The first study reveals that NMDA antagonism is sufficient to induce powerful low-frequency modulation of spiking activity and extracellular local field potential (LFP) and discusses the implications of this finding for …

The Role Of The Neurodevelopmental Disorder Gene Myt1l In Mammalian Brain Development, Jiayang Chen

Arts & Sciences Graduate Student Theses and Dissertations

Recent human genetic studies have associated mutations in a gene called Myelin Transcription Factor 1 Like (MYT1L) with neurodevelopmental disorders (NDDs). Patients with MYT1L loss of function (LoF) mutations (MYT1L Syndrome patients) demonstrate shared symptoms such as microcephaly, attention deficit and hyperactivity disorder (ADHD), and obesity. Despite prior studies showing MYT1L overexpression facilitates neuronal differentiation in vitro, its functions in vivo, especially in the mammalian brain, and how its mutation leads to human disease pathology remains poorly understood. Here, I established the first mouse model of MYT1L Syndrome mimicking a patient specific LoF mutation. I found mice with Myt1l heterozygous …

Genetic And Transcriptomic Aspects Of Major Depressive Disorder: In Vivo Functional Assays Of Risk-Associated Variation, Candidate Disease Cell Types, And Their Pharmacologic And Sex Interactions, Bernard Mulvey

Arts & Sciences Graduate Student Theses and Dissertations

Major depressive disorder (MDD) is a debilitating illness that affects hundreds of millions globally, with substantial personal, medical, economic, and societal consequences. While depression occurs more commonly in females, the biology of the brain and sex underlying this skewed prevalence remains unclarified. This body of work explores two aspects of how biological sex may influence the brain at the level of gene expression: through intrinsic sex differences and through sex-mediated effects of depression risk genetics.The monoamine hypothesis of depression suggests that modulatory neurotransmitters including serotonin and norepinephrine constitute a key axis in development of MDD. Large-scale studies of MDD treatment …

Quasiparticle And Excitonic Effects In Two-Dimensional Van Der Waals Materials, Linghan Zhu

Arts & Sciences Graduate Student Theses and Dissertations

Since their discovery, low dimensional van der Waals materials have attracted increasing research interests. They serve as ideal platforms to study novel physics in reduced-dimensional systems, and are critical in nowadays’ nanotechnology applications. Due to the reduced dielectric screening in low dimensions, strong excited state properties dictate their electronic, transport and optical properties, the study of which calls for a description of the many-particle interactions beyond the traditional density functional theory. This is where the many-body perturbation theory comes into play. In this thesis, I will present a comprehensive study of the quasiparticle and excitonic properties of a variety of …

Modernism As Public Art: Reawakening The Social Agency Of Abstract Public Sculpture, 1950–1980, Tola Porter

Arts & Sciences Graduate Student Theses and Dissertations

This dissertation presents a critical reassessment of postwar modern public sculpture and offers a framework for both the reevaluation and reawakening of these significant, inherited public images. Between 1950 and 1980, as a result of new national policies, the United Kingdom and the United States experienced a boom in public sculpture. That phenomenon was augmented by architectural modernism, whose stark buildings called for the addition of sculpture. The growing prestige of abstract art made it a popular choice for architects and commissioners, who sought to leverage the cachet of contemporary artists. For both private sector architects and public sector patrons, …

Functionalized Plasmonic Nanostructures For Ultrasensitive Single Cell Analysis, Priya Rathi

Arts & Sciences Graduate Student Theses and Dissertations

Ultrasensitive detection and quantification of soluble, secreted and cell surface-bound proteins is critical for advancing our understanding of cellular systems, enabling effective drug development, novel therapies, and bio-diagnostics. However, exiting technologies are largely limited by their sensitivity, making the detection and quantification of low-abundant proteins extremely challenging. This forms a major barrier in various fields of biology and biomedical sciences. In this work, we introduce novel cellular analysis methodologies based on plasmon-enhanced fluorescence for analyzing cell structure and probing surface and secreted proteins from cells. In the first part, we introduce plasmon-enhanced expansion microscopy and demonstrate the effectiveness of employing …

An Online Vignette Study To Examine The Outcomes Of A Preclinical Alzheimer Disease Diagnosis, Matthew John Wynn

Arts & Sciences Graduate Student Theses and Dissertations

As Alzheimer disease research forges ahead, and new potential treatments are developed, a conceptualization is emerging of a presymptomatic disease stage. This stage, known as preclinical Alzheimer disease, is characterized by the buildup of amyloid beta and tau proteins in the brain to abnormal levels in a cognitively normal person. There are unknown potential risks and benefits of communicating biological marker risk information for Alzheimer disease using the preclinical Alzheimer disease diagnostic label. The current study uses a vignette methodology to measure older adults’ understanding of risk information when presented with information regarding their risk for developing Alzheimer dementia. Participants …

Jurisprudence Rules Related To The Crime Of Electronic Extortion, Haifa Mohammed Abd

Alustath Journal for Human and Social Sciences

In the light of the rapid technological development that we live in, the world has become a small village, in which a lot of science and information has grown, and new problems have been born in it that are rapidly spreading like wildfire, the most prominent of which are (social communication problems), and they are many, and the most important of them is the problem of (extortion), which has become an assault on the sanctities of people and their reputation as a prominent feature in it, in which the weak of souls found a way to bargain in the exploitation …

Morphological And Grammatical Issues In The Interpretation Of (Liberation And Enlightenment) In Surat Yusuf From Verse (11 To 21), Zainab Jasim Hasan

Alustath Journal for Human and Social Sciences

Al-Taher bin Ashour (d. 1393 AH-1973 AD) is one of the brightest linguistic interpreters in the modern era, as he was interested in explaining the meanings of the Holy Qur’an about its hidden and deep implications, and revealing the subtleties of his knowledge and secrets of his eloquence. He wrote his famous book (Al-Tahrir and Enlightenment) in the interpretation of the Holy Qur’an, in which he applied the linguistic lesson. Ibn Ashour was not satisfied with explaining the verses and explaining the reasons for their revelation. Rather he listed the linguistic aspects with their refutation and discussion.

The importance of …

Testing The Ability Of Efl Postgraduate Certificate Teachers To Identify Vocabulary Idiomatic Meaning In Nineveh Schools, Hiba M. Yaqoob

Alustath Journal for Human and Social Sciences

Given the importance of the idiomatic meaning of expressions, especially for EFL teachers who have the responsibility of writing research, so the present study aims at testing the ability of (40) of EFL teachers in Nineveh schools who have post-graduate certificates and work in schools, to identify two types of the idiomatic meaning in some sentences. The researcher prepared a test consisting of (40) items; each item consisted of four options, one is correct. Two types of idioms are included in the test which is divided equally: idioms of encoding and idioms of decoding. After confirming the validity and reliability …

Roles Of Behavioral Novelty And Organismal Energetics In The Evolution Of Extreme Encephalization, Erika Laurie Schumacher

Arts & Sciences Graduate Student Theses and Dissertations

Brains and their component brain regions vary widely in size and structure across vertebrates. However, extreme increases in total brain size relative to body size (extreme encephalization) and increases in specific major brain region sizes independent of other brain regions (mosaic brain evolution) are relatively rare. There are several hypotheses as to why, but the energetic cost of increased brain tissue and the regional interconnectedness in both brain development and function likely constrain how brains are able to change in response to selection. In this dissertation, I sought to address both how behavioral novelty relates to evolutionary changes in brain …

Contribution To Data Science: Time Series, Uncertainty Quantification And Applications, Dhrubajyoti Ghosh

Arts & Sciences Graduate Student Theses and Dissertations

Time series analysis is an essential tool in modern world statistical analysis, with a myriad of real data problems having temporal components that need to be studied to gain a better understanding of the temporal dependence structure in the data. For example, in the stock market, it is of significant importance to identify the ups and downs of the stock prices, for which time series analysis is crucial. Most of the existing literature on time series deals with linear time series, or with Gaussianity assumption. However, there are multiple instances where the time series shows nonlinear trends, or when the …

Characterizing Complex Affective Processing Across Age And In Relation To Social Anxiety Symptoms, M. Catalina Camacho

Arts & Sciences Graduate Student Theses and Dissertations

Humans require a shared understanding of others’ emotions for adaptive social functioning. For instance, if someone tells a joke and another person laughs, we assume the laughing person was happy because we have a shared concept for such positive interactions. Children develop and refine these concepts as they grow up, but the underlying neural processing that supports this development is unknown. Identifying the neurodevelopment underlying emotion processing could provide important insight to emotional disorders. For example, social anxiety is associated differences in the detection and interpretation of negative or neutral emotional cues, but the underlying neurodevelopment of this behavior is …

Perceptual Experience, Representation, And The Environment, James Gulledge

Arts & Sciences Graduate Student Theses and Dissertations

Recent cognitive science suggests that the system responsible for perceptual experience is flexible, can learn, and is robustly affected by information stored in subjects’ minds. My dissertation is about the account of the mind and subjective awareness that emerges if this picture of perceptual experience is true. Whether perceptual experience is affected by stored information is usually tied to discussions of so-called ‘cognitive penetration’ (what I call cognitive ‘permeation’). I consider the focus on cognitive permeation largely as a red herring, instead holding that we should view the relevant epistemic issue more broadly: as that of whether changing stores of …

Laywoman Of Right Faith: The Religious Writings Of Wang Peihua (1767-1792), Meijie Shen

Arts & Sciences Graduate Student Theses and Dissertations

ABSTRACT OF THE DISSERTATIONLaywoman of Right Faith: The Religious Writings of Wang Peihua (1767-1792) by Meijie Shen Doctor of Philosophy in Chinese Language and Literature Washington University in St. Louis, 2022 Professor Beata Grant, ChairThis dissertation is a case study of an eighteenth-century Buddhist laywoman named Wang Peihua (1767-1792) from the affluent Jiangnan area of imperial China. This period saw the flourishing of women’s education and writings, thanks to which we have collections left behind by them that document their own lives and in their own voice, which enabled us to explore their religious experience. As women started to enter …

Behavioral, Neural, And Psychiatric Correlates Of Responses To Social Feedback, Brent Rappaport

Arts & Sciences Graduate Student Theses and Dissertations

Problems responding to peer feedback and disrupted interpersonal relationships arise in numerous psychiatric disorders; yet heterogeneity and homogeneity across disorders suggests both common and unique mechanisms of impaired social function. Identifying brain correlates of these disruptions could help explain diagnostic comorbidities as well as unique pathways, thus informing more individualized treatments. However, studies seeking to understand the link between psychological and neural mechanisms often have to rely on reverse inference assumptions to match psychological processes to brain activity. One hypothesis is that social feedback is processed similar to other forms of feedback (e.g., monetary). Thus, we aimed to test such …

Politics During And After Democratic Backsliding, Benjamin Rieth Schneider

Arts & Sciences Graduate Student Theses and Dissertations

Why do voters support backsliding incumbents? Under what conditions are voters more permissive to backsliding? In recent years, scholars have been interested in what leads to voters to support backsliding incumbents where we should expect them to be sanctioned and generally these answers have been focused on partisanship and polarization. I build on this literature by arguing that societal threat reduces the negative evaluation of backsliding actions and highlights the need for competent leaders to protect against future crisis. I develop an original formal model that considers the different strategies available to backsliding incumbents and shows that societal threat benefits …

Drawing From The Past, Pengpeng Chen

Theses

Drawing From the Past is a short, 3D animated film that portrays the different thoughts about drawing between two generations and is based on my personal experience. Some art students get less encouragement from their families than other students. In Asian traditional thinking, majoring in art was not the correct career direction, which is correct in that period， because of the economy. Majoring in art means students need to pay more money than other majors. In addition, art is not necessary for some people who worry about food. In the previous period, many people worry about their lives. That means …

Listening To First Generation College Students In Engineering: Implications For Libraries & Information Literacy, Emily Dommermuth, Linds W. Roberts

Communications in Information Literacy

First-generation college students (FGCS) in engineering bring a wealth of knowledge to their academic and social experiences in higher education, in contrast to deficit-based narratives that students are underprepared. By listening to FGCS’ own experiences navigating higher education and using information literacy in their project-based work, librarians and educators can better understand students’ funds of knowledge, social capital, and identities, as well as the institutional barriers that must be removed. This paper shares interview findings with (n = 11) FGCS and suggests implications for professional practice that are relevant to information literacy for design, project-based, or practitioner focused disciplines.

Working Paper No. 67, Insights Into Project Cybersyn, Leah Herrera

Working Papers in Economics

This inquiry seeks to establish that back in the 1970s Chile’s “Project Cybersyn” offered novel approaches and specific technologies that appeared to have benefitted capitalism as a system. The Spanish name, SYNCO served as an acronym for Sistema de Información y Control. President Salvador Allende expected that the attributes associated with Project Cybersyn could assist his efforts in implementing his variant of socialism. Cybersyn consisted of a network (Cybernet), software (Cyberstride), computers, a economic simulator known as CHECO, and a control room (Opsroom.) Cybersyn reached an advanced prototype stage; however, its fate was also tied to the interests of the …

Pnp Maxtools: Autonomous Parameter Control In Maxmsp Utilizing Mir Algorithms, Austin Alexander Franklin

LSU Doctoral Dissertations

This research presents a new approach to computer automation through the implementation of novel real-time music information retrieval algorithms developed for this project. It documents the development of the PnP.Maxtools package, a set of open source objects designed within the popular programming environment MaxMSP. The package is a set of pre/post processing filters, objective and subjective timbral descriptors, audio effects, and other objects that are designed to be used together to compose music or improvise without the use of external controllers or hardware. The PnP.Maxtools package objects are designed to be used quickly and easily using a `plug and play' …