Digital Commons Network^™

Uncloaking Cell-Impermeant Gold Nanorods Via Tumor Microenvironmental Cathepsin B Facilitates Cancer Cell Penetration And Potent Radiosensitization, Subhiksha Raghuram, Yuri Mackeyev, Jessica Symons, Yasmin Zahra, Valeria Gonzalez, Krishnan K Mahadevan, Katherinne I Requejo, Anton Liopo, Paul Derry, Eugene Zubarev, Onur Sahin, Joseph Byung-Kyu Kim, Pankaj K Singh, Sang Hyun Cho, Sunil Krishnan

Faculty, Staff and Student Publications

Major impediments to conveyance of intravenously administered drugs to tumors are biofouling, opsonization, and rapid clearance from the circulation by macrophages and reticuloendothelial phagocytes. Cloaking nanoparticles with stealth epilayers partly overcomes these hurdles but it also foils interactions with tumor cells. Here, we describe the synthesis, characterization, and validation of smart gold nanorods (GNRs) that spontaneously transform from inert passengers in the blood stream to active cell-penetrating nanoparticles within tumors to potently sensitize tumors to radiation therapy. Intrinsically cationic and cell-penetrating GNRs were shielded from phagocytosis with a cloaking polyethylene glycol epilayer containing an intervening cleavable peptide. In the absence …

Metabolic Changes Associated With Gestational Diabetes And Weight Loss, Marykate Smith

Master's Theses

Introduction: Gestational Diabetes Mellitus (GDM) is diabetes diagnosed for the first time during pregnancy and negatively affects the mother and her child. Research suggests a multidisciplinary lifestyle intervention may aid in the reduction of GDM and its complications. Further, the use of metabolomics is needed to understand the alterations that happen prior to GDM diagnosis to reduce its recurrence.

Objective: Metabolomics analysis was conducted at baseline and 16-weeks post-intervention for women enrolled in a pre-pregnancy lifestyle intervention study to observe metabolic changes associated with GDM occurrence and weight loss.

Methodology: Sixteen samples from the parent Gestational Diabetes Prevention trial were …

Dynamic Soil Properties In The Lower Rio Grande Valley: Understanding Responses Of Infiltration Rate, Bulk Density, Aggregate Stability To Different Tillage Practices, Temiloluwa Faith Awotoye

Theses and Dissertations

Dynamic Soil Properties (DSPs) are soil properties that change in response to both natural and human-caused interruptions and pressures such as agro-based and wildland management. These soil properties can transform for months or even years in response to the farm use and management procedures. This research project investigates the relationship between different dynamic soil physical properties and tillage practices in the Texas Hidalgo sandy clay loam benchmark soil series. Data analyses was done to compare the effect of tillage to these soil physical properties and build a discussion about relationships between on-farm practices to other aspects of farm viability. We …

Dynamic Soil Properties In The Lower Rio Grande Valley: Carbon And Nitrogen Responses To Different Tillage Practices, Luzyannet Ballesteros Gonzalez

Theses and Dissertations

There has been increased attention in utilizing agriculture for carbon sequestration. Soil carbon and nitrogen are two dynamic soil properties (DSPs) that are indicators of soil health and function and have overlapping cycles. These soil properties change frequently as a result of environmental conditions and agricultural management practices. This study focuses on the impact of tillage practices in unirrigated agricultural fields in Hidalgo and Willacy counties located in South Texas on Hidalgo Sandy Clay Loam; With a particular focus on Soil Total Carbon (TC), Soil Inorganic Carbon (SIC), Permanganate Oxidizable Carbon (POXC), Carbon and Nitrogen ratio (C:N), Soil Organic Matter …

Wildlife Road Mortality Patterns In South Texas And Survey Methodology Improvement, Bradley E. Beer

Theses and Dissertations

Mortalities of wildlife caused by collisions with vehicles along roads are increasing in prevalence, threatening the existence of various species and populations. A better understanding of how mortalities change in response to natural and anthropogenic variables and efficient methods of obtaining mortality data are essential to mitigating such mortalities. This thesis investigates several key elements to improving road mortality surveys in south Texas. First, it was found that road mortality survey counts did not change under a pandemic-related lockdown and that 2 mortality survey observers detect more mortalities than 1. Analysis of brown pelican groundings on Texas State Highway 48 …

The Molecular Evolution Of Insect Opsin Genes, Maria Jose Cardenas Muedano

Theses and Dissertations

Opsins are part of the superfamily of G-coupled receptor proteins and together with chromophores, are responsible for initiating the signal transduction cascade responsible for animal vision. Since opsins have a critical role on visual perception these genes are crucial for a myriad of adaptive traits and behaviors and should show diverse signs of natural selection at the molecular level. Understanding the evolution of opsins across the wide diversity of insect groups will allow us to better understand the evolutionary pressure on insect opsins and serve as a model for both other gene systems and other taxonomic groups of animals that …

Winds & Waves: A Collection Of Short Stories & Poems [Vientos Y Olas: Una Colección De Cuentos Y Poemas], Joann Balderrama

Theses and Dissertations

Winds & Waves: A Collection of Short Stories & Poems, presents works of fiction telling a cohesive story with themes of survival, joy in simplicity, and resilience. My work will show the gradual exposing of moods as well as experimentation with writing techniques in several genres. My writing has developed to include a voice of introspection.

My thesis title includes winds and waves to signify change and transformation. They also refer to the movement in nature. Nature is included in a lot of my pieces. Water is an important symbolic component in several of the pieces as well.

Being …

My Journey As An Elementary Science Teacher: From Linear To Authentic, Johanna Lynn Esparza

Theses and Dissertations

The purpose of this dissertation is to provide a personalized narrative of one science teacher’s use of reflexive teaching as an agent of change. This dissertation is about a journey of change in instruction fostered by a change of identity as a science teacher, using the butterfly cycle as a metaphor. This dissertation narrates the identity evolution of the teacher. This study has relevance because the process utilized by the teacher provides a method of self-examination and identity construction for other elementary science classroom teachers who want to improve their science practices. This study also has relevance because it describes …

Monsters & Men, Christopher Ernest Garcia

Theses and Dissertations

Monsters & Men is a collection of poetry and illustrations that explores the complex emotions associated with masculinity, grief, and personal development. I achieve this by heavily reflecting on my relationship with my late father and using this medium as an outlet to express my trauma in a healthy manner. How we perceive ourselves as men, and what is possible, is not reflected enough in society and I aim to be a proponent in stimulating the conversation revolving around men’s mental health. Men and the patriarchy impose themselves on others to the point where we need to address the unethical …

Emotion Identification Ability And Perspective-Taking In Children With Autism Spectrum Disorder, Ana Lucia Garrido Huerta

Theses and Dissertations

Autism spectrum disorder (ASD) is characterized by persistent deficits in social interaction. Perspective-taking and emotion identification are important skills to develop appropriate social interactions. Previous research has shown that children with ASD may lack the ability to relate emotionally to others. Research has also shown a deficit in perspective-taking skills in children with ASD. The selected studies reviewed in this thesis analyze seven articles that show research on children with ASD regarding their emotional identification and perspective-taking skills. Articles searched under the words “emotion identification,” “perspective-taking,” and “autism” obtained from the Psychology and Behavioral Sciences Collection using the EBSCO database …

Salas Y Gómez Benthic Habitat And Community Assessment Of Seamounts And Oceanic Islands, Kara Eckley

Theses and Dissertations

Seamounts are essential benthic habitats that are ecologically important, providing habitat and functioning as stepping stones for dispersal and possible refugia, and economically valuable, supporting deep-sea fisheries and potential mineral mining activities. The Salas y Gómez Ridge in the southeast Pacific Ocean longitudinally contains dozens of seamounts that extend ~2,900 km across the South Pacific Subtropical Gyre. The ridge contains a range of oxygen and nutrient conditions that, along with the distance between seamounts, have led to high levels of endemism, reported in the few studies that have been conducted on seamounts of the region; this study is the first …

Atomlbs: An Atom Based Convolutional Neural Network For Druggable Ligand Binding Site Prediction, Md Ashraful Islam

Theses and Dissertations

Despite advances in drug research and development, there are few and ineffective treatments for a variety of diseases. Virtual screening can drastically reduce costs and accelerate the drug discovery process. Binding site identification is one of the initial and most important steps in structure-based virtual screening. Identifying and defining protein cavities that are likely to bind to a small compound is the objective of this task. In this research, we propose four different convolutional neural networks for predicting ligand-binding sites in proteins. A parallel optimized data pipeline is created to enable faster training of these neural network models on minimal …

Development Of Vegetable Oil-Based Nano-Lubricants Using Ag, H-Bn And Mgo Nanoparticles As Lubricant Additives, Kollol Sarker Jogesh

Theses and Dissertations

Because of the harmful impact of petroleum-based lubricant on the environment and human body, vegetable oil-based lubricant with eco-friendly nanoparticles has a great potential to be an alternative lubricant if it possesses proper lubricating properties. In this study, thermal conductivity, viscosity and tribological properties (wear scar diameter and coefficient of friction) of vegetable oil-based nanolubricant, developed from soybean oil and sunflower oil, modified with Ag, h-BN and MgO nanoparticles as lubricant additives, were evaluated. For thermal conductivity evaluation, a line heat source method was used with KD2 Pro-Thermal Property Analyzer. For viscosity evaluation, Haake Mars 40-rheometer was used to evaluate …

Effects Of Roundup Exposure On Redox Status, Cellular Apoptosis, And Antioxidant And Osmoregulatory Enzyme Expressions In Goldfish (Carassius Auratus), Md Imran Noor

Theses and Dissertations

Intense anthropogenic activities of industrialized nations dramatically increase environmental pollution. This study focused on the effects of Roundup, a glyphosate-based herbicide, exposure (low- and high-dose: 0.5 and 5 mg/L for 2 weeks) on dinitrophenyl protein (DNP), nitrotyrosine protein (NTP), superoxidase dismutase (SOD), catalase (CAT), Na+ /K+ - ATPase (NKA), and renin expressions, and cellular apoptosis in the gills and kidneys of goldfish. Histopathological analysis showed widespread tissue damage in both gills and kidneys. Immunohistochemical analysis provided insights into the expression of molecular biomarkers in tissues. Fish exposed to Roundup exhibited a significant (P<0.05) upregulation in DNP, NTP, SOD, and CAT expressions, and apoptotic nuclei in both tissues. Additionally, exposure to Roundup significantly increased renin expression in kidneys and decreased NKA expression in gills. Overall, our results suggest that exposure to Roundup induces oxidative/nitrative stress and cellular apoptosis and alters osmoregulatory and antioxidant systems which may lead to impaired physiological functions in goldfish.

Incorporating Native Plants In Insectary Strips To Promote Insect Diversity And Below-Ground Beneficial Microbes In South Texas, Lindsey N. Richards

Theses and Dissertations

Farm edges are generally the most undisturbed areas in a farm with diverse vegetation and can enhance agrobiodiversity and provide crucial food and shelter for wildlife, insects, and soil biota. Planting native wildflowers with the ability to reseed and withstand local climatic conditions for semi-permanent vegetation around farm edges or in between crops has potential to biologically control pests. To test this, we ran a two-year study and installed three different flowering insectary strips on a certified organic vegetable farm in South Texas during the winter season. The treatments were: 1) a commercially sourced 17-species native wildflower seed mix; 2) …

An Evaluation Of Media Messaging By African Fashion Designers And Marketing Message Strategies, Esther Oyindamola Oyanibi

Electronic Theses and Dissertations

In recent times, African brands have been featured heavily in global landscape fashion events, with brands from Nigeria, South Africa, and Kenya leading the way. Although such recognition could be traced to their expertise, the unique approach of African brands involves putting out unique designs that easily stand out in the global fashion market. However, their uniqueness goes beyond the designs they put out, as it is also strongly expressed in their media messaging and, consequentially, their marketing strategy.

This study provides a content analysis of the most important characteristics of eight brands from different regions of the African continent. …

A Frayed Edge: A Qualitative And Poetic Inquiry Analysis Of White Antiracist Protest In 2020, Emily Katt

Electronic Theses and Dissertations

This multiphasic study explored the narratives of five first-time Black Lives Matter protesters demonstrating during the historic confluence of conflicts in 2020 America. After positioning the liminal 2020 circumstances within an antiracist research lens, the author analyzed, first through grounded theory and then secondarily through poetic inquiry, how these five participants described their protest experiences. The grounded theory phase yielded an overarching theory that first-time protestors experienced a dual process of unsuturing and of calling-out, with three subthemes categorized within each of these two processes. The author moved into analysis with the poetic inquiry phase, crafting poems guided by six …

Carceral Intent, Danielle C. Jefferis

Michigan Journal of Race and Law

For decades, scholars across disciplines have examined the stark injustice of American carceralism. Among that body of work are analyses of the various intent requirements embedded in the constitutional doctrine that governs the state’s power to incarcerate. These intent requirements include the “deliberate indifference” standard of the Eighth Amendment, which regulates prison conditions, and the “punitive intent” standard of due process jurisprudence, which regulates the scope of confinement.

This Article coins the term “carceral intent” to refer collectively to those legal intent requirements and examines critically the role of carceral intent in shaping and maintaining the deep-rooted structural racism and …

The Effect Of Tharthar-Tigris Canal On The Environmental Properties Of The Tigris River Northern Baghdad, Iraq, Osama S. Majeed, Ahmed J. M. Al-Azawi, Muhanned R. Nashaat

Baghdad Science Journal

The present study is considered the first on this sector of the Tigris River after 2003. It is designed for two aims, the first is to demonstrate the seasonal variations in physicochemical parameters of Tharthar-Tigris Canal and Tigris River; the second is to explain the possible effects of canal on some environmental properties in the Tigris River. Water samples were being collected monthly. Six sampling sites were selected, two on Tharthar Canal and four along the Tigris River, one before the confluence as a control site and the others downstream the confluence with the canal. For a period from January …

Heat Waves And A Public-Private Partnership In Alaska, Karen Sandrik, Sarah Matsumoto

Alaska Law Review

The recently-passed Inflation Reduction Act represents the largest single step that Congress has taken to combat harms from climate change. In its over $360 billion commitment, the Act incentivizes clean energy development and generation, includes methods to directly lower residential utility bills and increase home efficiency, promotes cleaner transportation and agricultural practices, and funds states' and cities' efforts to meet their individual climate goals. While many environmental organizations applauded the Act's passage, some—even simultaneously—expressed concern about its tradeoffs: the Act continues to invest in fossil fuels, subsidizing pipeline construction and guaranteeing new oil and gas leases, specifically expanding leasing in …

Protecting Subsistence Lands While Boosting The Bottom Line: The Enhanced Federal Tax Incentive Available To Alaska Native Corporations For Donations Of Conservation Easements, Timothy Troll, Konrad Liegel

Alaska Law Review

Alaska Native corporations face a dilemma. They own land of immense and significant cultural and ecological value. Their lands are critical for maintaining the Alaska Natives' subsistence needs. But they are also corporations established under the law to maximize the economic value of their land holdings to provide financial dividends to their Native shareholders. This paper explores the enhanced federal tax incentive for donations of perpetual conservation easements that became available in 2015 to Alaska Native corporations. The tax incentive offers Alaska Native corporations a way to protect the aboriginal lands conveyed to them under the Alaska Native Claims Settlement …

Coastal Marine Debris In Alaska: Problems Withplastics, Pollution, & Policy, Savannah Artusi

Alaska Law Review

Plastics pollute people and the planet throughout their lifecycle, from intensive extraction of raw materials to chemical leaching during their use to entangling animals in discarded plastic products. Plastic waste is especially troublesome in Alaska, where the state's extensive shoreline and coastal communities are disproportionately inundated with plastic marine debris. Current policies internationally, in the United States, and in Alaska have not done enough to prevent plastic waste from ending up on Alaska's coasts, to hold plastic producers accountable for that waste, or to provide Alaskan communities the support needed to remove the waste themselves. This Note offers several proposals …

The Kenai Rule In Four Acts: Bear Baiting, Firearms, And Hunting: Comment & Analysis Of Alaska V. Bernhardt, Jon C. Nachtigal, Mike Stocz

Alaska Law Review

The Kenai Rule, enacted by the U.S. Fish and Wildlife Service in 2016, prohibits (a) the hunting of brown bears with bait in the Kenai National Wildlife Refuge, (b) most hunting in the Skilak Wildlife Recreation Area, and (c) the discharge of firearms along the Kenai and Russian Rivers. The Kenai Rule was challenged by the State of Alaska and Safari Club International in Alaska v. Bernhardt. This Comment provides an overview of the case as it was heard in the District Court of Alaska. This discussion includes arguments and counterarguments surrounding the application of four legislative acts: the …

Improving Parental Education, Daisy Martinez Ortiz

Capstone Projects and Master's Theses

Monterey County has one of the highest records of child maltreatment that leads to life-long consequences. COVID-19 has significantly impacted the way parent education classes are being delivered. Without the proper parental education, child maltreatment increases. Parental stress has also greatly contributed. All the stress brought among parents also posed as a factor for increased violence and child abuse. Low levels of parental education results in poor parenting. Poor parenting greatly affects a child’s social, emotional, and intellectual development. Community Human Services is a nonprofit organization dedicated to addressing a variety of issues. Among their different departments, under their mental …

Voltage-Gated Potassium Channel Dependent Mechanisms Of Cardiovascular Adaptation To Chronic Exercise., Sean Raph

Electronic Theses and Dissertations

Exercise increases cardiac workload, escalating the demand for oxygen. The myocardial vasculature responds to the greater demand for oxygen by increasing blood flow to match the needs of the heart during augmented work. Increasing blood flow requires the coronary arteries dilate (a.k.a. vasodilation); this is mediated predominantly by vascular smooth muscle cell relaxation. Vasodilation is driven through inhibition of calcium influx into vascular smooth muscle cells. The prevention of calcium influx is largely mediated by efflux of potassium via potassium channels causing membrane hyperpolarization, which in turn closes voltage-dependent (or gated) calcium channels (VDCC). Changes in vascular smooth muscle cell …

A Dialogical Approach To Peer Coaching For Asset Pedagogies, Elisabeth Van Beek

Dissertations

The purpose of this study was to understand how dialogical peer coaching using an asset pedagogical framework supported the development of culturally sustaining pedagogy (Gay, 2000; Ladson-Billings, 1994; Paris & Alim, 2017). This action research study followed one teacher and eight emergent bilingual students during an intervention. The intervention consisted of asset projects developed from the funds of identity framework (Esteban-Guitart, 2016). The peer coaching process consisted of a period of observation followed by repeated co-planning, coteaching, and reflection. The researcher took a dialogical stance on creating asset projects with the students as well as peer coaching with the teacher …

College Students’ Perceptions Of The Desert Canyons Of The Colorado Plateau, Jeremy Roger Deem

Dissertations

This study investigates eight college students’ perceptions of their experiences during an accredited outdoor education course entitled Technical Canyoneering. The course took place in four separate desert canyons of the Colorado Plateau, two in Arches National Park, and two on nearby Bureau of Land Management public space. Utilizing the data collection process of photo-elicitation, the participants’ time spent in the canyon settings was described in order to gain an understanding of their experiences, with particular attention paid to analyzing what perceptions those students ascribed to those experiences. The intent was threefold. First, the study makes understanding at how college students …

The Distribution And Reproductive Success Of The Western Snowy Plover Along The Oregon Coast - 2022, David J. Lauten, Kathleen A. Castelein, Daniel Farrar, Mary Lee, Eleanor P. Gaines

Institute for Natural Resources Publications

We monitored the distribution, abundance and productivity of the federally threatened Western Snowy Plover (Charadrius nivosus nivosus) along the central and south coast of Oregon from 1 April – 15 September 2022. We surveyed and monitored plover activity in a project area that included, from north to south, Sutton Beach, Siltcoos River estuary, the Dunes Overlook, North Tahkenitch Creek, Tenmile Creek, Coos Bay North Spit, Bandon Snowy Plover Management Area, New River Habitat Restoration Area (HRA) and adjacent lands, and Floras Lake. Our objectives for the project area in 2022 were to: 1) estimate the size of the adult Snowy …

A Cloning-Free Recyclable System For Crispr-Cas9 Mediated Mutant And Reversion Construction In Candida Albicans Clinical Isolates, Amanda K. Vogel

Theses and Dissertations (ETD)

Candida albicans is a ubiquitous opportunistic fungal pathogen and one of the most prevalent causes of fungal diseases worldwide. The reference isolate SC5314 is one of the most widely used strains for both experimental and genetic studies, but it is becoming increasingly evident that genetic diversity in clinical isolates plays an important role in antifungal resistance, virulence, and pathogenicity. These recent discoveries highlight the need for genetic tools that are capable of investigating genes in multiple strain backgrounds. Here we build on the SAT1-flipper method and combine it with CRISPR-Cas9 technology to achieve cloning-free homozygous deletion in a single transformation …

A Clean Slate: Green Slate Production And Exchange In The Mojave Desert, Jamie Marie Nord

Electronic Theses, Projects, and Dissertations

In this thesis, I examine the procurement, manufacturing process, and subsequent distribution of cultural greenstone artifacts, historically referred to as green slate, in the Mojave Desert of southeastern California from a landscape-level framework. The San Bernardino County Museum (museum) curates a collection of incised and blank green slate artifacts (n=51) from numerous archaeological sites in the study region. These cultural materials were uncovered together in a box during routine inventory. As part of this thesis, I catalogued, rehoused, and remarried the collection with each artifact’s respective site assemblage in consultation with San Manuel Band of Mission Indians (SMBMI) in order …