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Effect Of Sowing Time On Phytomass Production During Early Growth Of Two Varieties Of Stylosanthes Guianensis (Aubl.) Sw., M. L.S. Paterniani, T. J. D. Rodrigues, G. T. Pereira, L. R. A. Rodrigues

IGC Proceedings (1977-2023)

The objective of this research was to determine the effect of two sowing times on phytomass production of two varieties of Stylosanthes guianensis (var. pauciflora and var. vulgaris). Two experimental periods were studied (1: January - May/1998 and 2: November/1998 - March/1999) using a completely randomized factorial design 2 x 2 x 14 (two periods, two varieties and fourteen ages of evaluation), with four replications. The results showed a difference between the periods concerning the growth and development of Stylosanthes, and that period 2 was the most favourable to this forage plant. There was, also, different adaptability between …

Silk-Cellulose Acetate Biocomposite Materials Regenerated From Ionic Liquid, Ashley Rivera-Galetti, Chrtstopher R. Gough, Farhan Kaleem, Michael Burch, Chris Ratcliffe, Ping Lu, David Salas-De La Cruz, Xiao Hu

College of Science & Mathematics Departmental Research

The novel use of ionic liquid as a solvent for biodegradable and natural organic biomaterials has increasingly sparked interest in the biomedical field. As compared to more volatile traditional solvents that rapidly degrade the protein molecular weight, the capability of polysaccharides and proteins to dissolve seamlessly in ionic liquid and form fine and tunable biomaterials after regeneration is the key interest of this study. Here, a blended system consisting of Bombyx Mori silk fibroin protein and a cellulose derivative, cellulose acetate (CA), in the ionic liquid 1-ethyl-3-methylimidazolium acetate (EMIMAc) was regenerated and underwent characterization to understand the structure and physical …

Cardiac Cellularity Is Dependent Upon Biological Sex And Is Regulated By Gonadal Hormones., Galen T Squiers, Michael A Mclellan, Alexei Ilinykh, Jane Branca, Nadia Rosenthal, Alexander R Pinto

Faculty Research 2021

AIMS: Sex differences have been consistently identified in cardiac physiology and incidence of cardiac disease. However, the underlying biological causes for the differences remain unclear. We sought to characterize the cardiac non-myocyte cellular landscape in female and male hearts to determine whether cellular proportion of the heart is sex-dependent and whether endocrine factors modulate the cardiac cell proportions.

METHODS AND RESULTS: Utilizing high-dimensional flow cytometry and immunofluorescence imaging, we found significant sex-specific differences in cellular composition of the heart in adult and juvenile mice, that develops postnatally. Removal of systemic gonadal hormones by gonadectomy results in rapid sex-specific changes in …

Thousands Of Cpgs Show Dna Methylation Differences In Acpa-Positive Individuals., Yixiao Zeng, Kaiqiong Zhao, Kathleen Oros Klein, Xiaojian Shao, Marvin J. Fritzler, Marie Hudson, Inés Colmegna, T Pastinen, Sasha Bernatsky, Celia M T Greenwood

Manuscripts, Articles, Book Chapters and Other Papers

High levels of anti-citrullinated protein antibodies (ACPA) are often observed prior to a diagnosis of rheumatoid arthritis (RA). We undertook a replication study to confirm CpG sites showing evidence of differential methylation in subjects positive vs. negative for ACPA, in a new subset of 112 individuals sampled from the population cohort and biobank CARTaGENE in Quebec, Canada. Targeted custom capture bisulfite sequencing was conducted at approximately 5.3 million CpGs located in regulatory or hypomethylated regions from whole blood; library and protocol improvements had been instituted between the original and this replication study, enabling better coverage and additional identification of differentially …

Use Of Immunohistochemistry To Determine Expression Of Rab5 Subfamily Of Gtpases In Mature And Developmental Brains, Kwok Ling Kam, Paige Parrack, Marcellus Banworth, Sheeja Aravindan, Guangpu Li, Kar Ming Fung

Books and Book Chapters

Rab GTPases are essentially molecular switches. They serve as master regulators in intracellular membrane trafficking from the formation and transport of vesicles at the originating organelle to its fusion to the membrane at the target organelle. Their functions are diversified and each has their specific subcellular location. Their expression may vary significantly in the same cell when the level of protein production is significantly different in different physiologic status. One of the best examples is the transition from fetal to mature status of cells. Expression and localization of Rab GTPases in mature and developing brains have not been well studied. …

Stable Thermally-Modulated Nanodroplet Ultrasound Contrast Agents, Anastasiia Vasiukhina, Javad Estraghi, Adib Ahmadzadegan, Craig J. Goergen, Pavlov P. Vlachos, Luis Solorio

School of Mechanical Engineering Faculty Publications

Liquid perfluorocarbon-based nanodroplets are stable enough to be used in extravascular imaging, but provide limited contrast enhancement due to their small size, incompressible core, and small acoustic impedance mismatch with biological fluids. Here we show a novel approach to overcoming this limitation by using a heating–cooling cycle, which we will refer to as thermal modulation (TM), to induce echogenicity of otherwise stable but poorly echogenic nanodroplets without triggering a transient phase shift. We apply thermal modulation to high-boiling point tetradecafluorohexane (TDFH) nanodroplets stabilized with a bovine serum albumin (BSA) shell. BSA-TDFH nanodroplets with an average diameter under 300 nanometers showed …

Ixekizumab For Patients With Plaque Psoriasis Affected By Multiple Sclerosis: Case Report, Enrique Herrera-Acosta, Gustavo G. Garriga-Martina, Jorge A. Suárez-Pérez, Eliseo A. Martínez-García, Enrique Herrera-Ceballos

Sultan Qaboos University Medical Journal

Multiple sclerosis is an autoimmune demyelinating disorder of the central nervous system that shares similar immunopathogenic mechanisms with chronic plaque psoriasis, such as the overexpression of the Th17 pathway. We report the case of a patient with multiple sclerosis and severe chronic plaque psoriasis successfully treated with ixekizumab (anti IL-17A and IL-17A/F monoclonal antibody). The treatment achieved a complete skin clearance (PASI 100 response) with no adverse events or evidence of progression of the neurological disease.

Effects Of Patch Size, Fragmentation, And Invasive Species On Plant And Lepidoptera Communities In Southern Texas, James A. Stilley, Christopher A. Gabler

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Simple Summary

Human land use has removed habitats, separated habitats into small and disconnected fragments, and introduced foreign species, which all harm wildlife. South Texas is highly diverse and home to many endangered species, but human disturbance threatens its wildlife. In south Texas, we poorly understand how different aspects of human land use influence wildlife diversity and abundance. We studied this by surveying plants and butterflies in 24 habitat fragments in south Texas that differed in size, shape, type, and land use history. Human disturbance was extensive, and foreign and weedy species were dominant in most habitats. Habitat types had …

Influence Of Water Stress On Protein Concentration In Themeda Triandra Forsk. In A Semi-Arid Climate Of South Africa, I. B. Oosthuizen, Hennie A. Snyman

IGC Proceedings (1977-2023)

The objective of this study was to determine the influence of four water treatments (T1 = 75-100%, T2 = 50-75%, T3 = 25-50% and T4 = 0-25% of Field Water Capacity) on the concentration of water-soluble protein as growth reserve during three growth stages (vegetative, pipe and reproductive) in three plant parts (roots, stubble and leafs). The water-soluble protein concentration (WSPC) in the plants under the four water treatments differed (P≤0.01) among the plant parts as well as among growth stages (P≤0.05). WSPC in leafs increased (P≤0.01) with increased water stress from T1 to T4 for the pipe growth stage. …

Effects Of Photoperiod, Low Temperature And N Nutrition On Vsp Accumulation In Taproot Of Alfalfa, J. C. Avice, C. Noquet, A. Ourry, J. J. Volenec

IGC Proceedings (1977-2023)

In Medicago sativa L., vegetative storage protein (VSP), specifically accumulated in taproot, are strongly involved in nitrogen storage. How the accumulation of such VSPs is regulated remains largely unknown. Experiments were designed with non-nodulated alfalfa to determine if length of the photoperiod, a decrease of temperature, or high availability of mineral nitrogen may induce the accumulation of VSPs. ¹⁵N labelling was used to quantify nitrogen uptake and its further relative translocation within the plant while VSPs accumulation was analysed by ELISA quantification. Results showed that environmental factors such as shortening daylength or low temperature changed biomass allocation within the …

Fructans From Elongating Leaf Bases Are A Source Of Carbon For Regrowth After Defoliation In Lolium Perenne, A. Morvan-Bertrand, J. C. Avice, M. P. Prud'homme

IGC Proceedings (1977-2023)

A detailed study of carbohydrate metabolism in perennial ryegrass (Lolium perenne L.) during the first 48 h of regrowth showed that the decline in fructan concentration occurred not only in the differentiation zone (30-60 mm from leaf base), but also in the elongation zone of the elongating leaf bases. Unlike other soluble carbohydrates, the net deposition rate of fructose remained positive and even rose during the first day following defoliation. FEH (fructan exohydrolase) activity, which was maximum in the differentiation zone before defoliation, increased in all segments but peaked in the elongation zone after defoliation. Taken all together, these …

Effect Of Climatic Factors On The Plant Population Dynamics In Temperate Pasture Implantation, B. Martin, R. Refi, S. Montico, M. Costanzo

IGC Proceedings (1977-2023)

The objective of this experiment was to determine the environmental influence on seedling emergence of alfalfa and fescue in mixture. We hypothesized that the temperature and precipitation data could be used to estimate field emergence of alfalfa and fescue. A field trial was carried out at Zavalla, Argentina and consisted of sowing a tall fescue – alfalfa mixture at different seeding date (March, April and June). Relationships between the range of temperature, rainfalls and alfalfa and fescue seedling emergence were described by linear regression models. Patterns of emergence and death of alfalfa and fescue seedling were found in the different …

Preparation Of Meta-Nitrobenzoylhydrazone Ferrocenoylacetone And Synthesis On Its Basis, Zilola Abduraxmonovna Sulaymonova, Bako Bafayevich Umarov Doctor Chemical Science, Professor

Chemical Technology, Control and Management

β-diketone-ferrocenoylacetone was obtained by Claisen condensation. Meta-nitrobenzoylhydrazone of ferrocenoylacetone (H₂L) was synthesized by the interaction of meta-nitrobenzoic acid hydrazide with ferrocenoylacetone. On its basis, complexes with copper(II) and nickel(II) ions were synthesized. The obtained compounds were characterized by the methods of elemental analysis, IR-, ¹H NMR andelectronic spectroscopy. The research results showed that H₂L in solution exists in the form of a tautomeric mixture: hydrazone, α-hydroxyazine, and cyclic 5-hydroxypyrazoline forms. According to the results of IR and ¹H NMR spectra, the complexes were assigned a planar-square structure, and in them the doubly deprotonated …

Application Of Methods Of Intermittent Ventilation Of Industrial Premises Using A Digital Data Transmission System, Yuriy Shipulin, Abdullayev Temur, Jamolidin Usmanov, Shahnoza Ergasheva

Chemical Technology, Control and Management

The article is devoted to topical applied issues, the application of methods of intermittent ventilation of industrial buildings using a digital data transmission system. The requirements for the parameters of the environment are determined, which include: composition, purity, temperature, air humidity, the content of positive and negative ions, the presence of fields of various origins (thermal, optical, biological, gas, and electromagnetic). The authors of the article argue that some of the listed parameters can be supported by ventilation and air conditioning (SCA) systems.

It is shown that when the controlled parameters are limited to a multidimensional area, the SCA control …

International Symposium Will Probe Berlin And Cold War, Impact On History And Import For The Future, Ohio University College Of Arts & Sciences

All Forum Articles

No abstract provided.

Growth, Maturation, And Weight Status: Insights From A Longitudinal Cohort Of Nebraska Youth, John William Masker

Kinesiology and Sport Sciences Theses, Dissertations, and Student Creative Activity

Pediatric obesity continues to be a major public health concern in the United States, with well-known short-term and long-term consequences. In efforts to combat pediatric obesity and identify children at high-risk for potential health problems, physicians and health professionals widely practice screening and classifying weight status using age-and-sex-specific body mass index (BMI) percentiles. However, some studies suggest the use of BMI for establishing weight status in relation to health risk in youth is problematic, especially during the period of the adolescent growth spurt. More importantly, maturation-related misclassification may result in overestimations of overweight prevalence among early-maturing youth, and underestimations of …

Analysis Of The Main Chemical And Volatile Components Of Xianggan Tea, Xiang-Style Ganpu Tea, Li Xiang, Fu Fu-Hua, Pan Zhao-Ping, Wang Chen, He Shuang

Food and Machinery

Objective: Xianggan tea, was made from Hunan Anhua dark tea, i. e. Tianjian tea and the mandarin peel of Yueyang Jinpen pomelo, based on the preparation method of Ganpu tea, and this contributed to ascertaining the quality of Xianggan tea. Methods: The main chemical components, mineral element content and volatile components were analyzed in the Tianjian tea, mandarin peel and Xianggan tea. Results: The moisture content of Tianjian tea, mandarin peel, and Xianggan tea were all less than 20%, and made them easy for storage. The content of crude polysaccharides, total flavonoids, and total phenols was in the order of …

Active Components, Antioxidant Capacity And Quality Evaluation Of Sichuan Dark Tea, Zhu Bo-Yu, Xia Chen, Luo Ke-Bin, Zhu Yong-Qing, Tang Xiao-Bo, Chen Jian

Food and Machinery

Objective: The contents of tea polyphenol monomer, caffeine (CAF), total polyphenols acid (TPC) and total flavonoids (TFC) in 26 kinds of commercial Sichuan dark tea (S1～S26) were analyzed, and the in vitro antioxidant activities were investigated, to provide a theoretical basis for the quality evaluation of Sichuan dark tea. Methods: Gradient elution was used to analyze the content of tea polyphenol monomer and caffeine. The contents of TPC and TFC were determined by the folin phenol method and chemical analysis method, respectively. The antioxidant activity was evaluated by DPPH and ABTS radical scavenging capacity. Results: The content of gallic acid …

Adsorption Of Caffeic Acid In Sucrose Solution By Magnetic Chitosan Modified Kaolin, Pan Yan-Mei, Wu Hai-Ling, Wei Qiao-Yan, Su Long, Liu Fu-Jie

Food and Machinery

Objective: Studied the adsorption characteristics of magnetic chitosan modified kaolin on caffeic acid in simulated sugarcane juice, and provided the reference for the removal of phenolic pigments in sugarcane juice. Methods: Magnetic chitosan modified kaolin was prepared by coprecipitation of chitosan, nano ferric oxide and kaolin. The surface structure and group changes of kaolin before and after modification were characterized by FT-IR, VSM and SEM; The adsorption characteristics of caffeic acid were studied by adsorption test. Results: the modified chitosan and nano-Fe₃O₄ were successfully loaded on kaolin, and the adsorption performance of caffeic acid was improved. The …

Determination Of 10-Hydroxy-2-Decenoic Acid In Honey By Ultra High-Performance Liquid Chromatography-Tandem Mass Spectrometry, Hu Li-Li, Li Ying, Wang Hui, Pi Lu-Lu, Chen Xian-Gui, Jiang Li

Food and Machinery

Objective: This study aimed to establish a method using ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) to determine 10-hydroxy-2-decenoic acid in honey. Methods: The samples were extracted with methanol-water and cleaned up by a PLS solid-phase extraction column. The separation was performed on an Agilent ZOXBAR SB-C18 column (2.1 mm×50 mm, 1.8 μm) and methanol -5 mmol/L ammonium acetate solution was used as the mobile phase for gradient elution. Mass spectrometry was carried out under the multiple reaction monitoring (MRM) mode with negative electrospray ionization (ESI-) and standard external method for quantitative analysis. Results: Good linearity of the developed analytical method …

Simultaneous Rapid Determination Of 18 Quinolones Residues In Raw Milk By Liquid-Liquid Extraction And Ultra Performance Liquid Chromatography Tandem Mass Spectrometry, Zhou Yan-Hua, Li Tao, Pan Xiao-Hong, Xiang Jun

Food and Machinery

Objective: A rapid method for simultaneous determination of 18 quinolones residues in raw milk was established. Methods: The raw milk was pretreated by liquid-liquid extraction, and the residues of 18 quinolones in raw milk were analyzed by ultra-performance liquid chromatography-tandem mass spectrometry. Results: The linearity of all the 18 quinolones from 2.0 to 100.0 ng/mL had correlation coefficients greater than 0.992. The limits of detection (LOD) of this method ranged from 0.02 to 0.50 μg/kg, and the limits of quantification (LOQ) were 0.06 to 1.50 μg/kg. The average recoveries of the 18 quinolones were 74.0% to 106.5%, with the relative …

Rapid Determination Simultaneously Of Twelve Kinds Of Nitrophenolic Compounds In Water By Modified Quechers-Gc-Ms, Wu Jian-Qiang, Zhao Zhong-Jing, Hong Xia, He Hai-Ning, Yuan Cai-Xia

Food and Machinery

Objective: A simple, efficient, rapid, sensitive, reproducible, accurate and rapid method was established for the simultaneous determination of 12 nitrophenols in water. Methods: Magnetic Fe₃O₄ nanoparticles loaded with bifunctional octadecylamine functionalized carbon nanotube composites were synthesized by coprecipitation and hydrothermal methods. The sample pretreatment method of QuEChERS was optimized and combined with gas chromatography-mass spectrometry to establish a method for the simultaneous determination of 12 kinds of nitrophenols in water quality. Results: The optimized QuEChERS sample pretreatment method gave the advantages of magnetic nanoparticles and carbon nano materials. Under the control of these conditions, high efficiency of …

Solid-Phase Extraction And Accurate Analysis On Food Additives In Marinated Meat Products, Wang Xiao-Jun, Li Feng-Ling

Food and Machinery

Objective: To establish an accurate method for the determination of benzoic acid, sorbic acid and saccharin sodium in soy sauce brine meat products. Methods: The samples were extracted by ethyl acetate, SPE-MCX solid phase extraction, and detected by HPLC simultaneously. Results: The determination was performed on Agilent Zorbax Eclipse XDB-C18 column （250 mm×4.6 mm, 5 μm） with mobile phase consisting of 75% 0.02 mol/L ammonium acetate solution and 25% acetonitrile. The column temperature was set at 25 ℃, and the flow rate was 0.8 mL/min. The sample size was 10 μL. The detection wavelength was 230 nm. In the range …

Effects Of Different Technologies On Enzyme Deactivation And Storage Stability Of Wheat Germs, Zhang Nan, Shi Lin, Meng Ji-Kun, Ge Xin-Hui, Cheng Yong-Qiang, Zhang Xiu-Qing

Food and Machinery

Objective: To obtain the best technology and treatment conditions of wheat germs stablizaiton, three different technologies were used to treat wheat germs. Methods: Microwave, superheated steam and hot air drying were used to treat wheat germs with enzyme deactivation, and their deactivation effects on lipase and lipoxygenase in wheat germs were compared. Then, the optimal treatment conditions of each technology were selected for the determination of total number of colonies and accelerated storage test. Results: Under the optimal treatment conditions, the lipase and lipoxygenase in wheat germs could be effectively deactivated by the three treatments. The superheated steam treatment had …

Effect Of Chlorine Dioxide Concentration On Storage Quality Of Cuihong Plum, Zhao Zhi-Bing, Liu Yong-Ling, Li Ying, Ba Liang-Jie, Luo Dong-Lan

Food and Machinery

Objective: This study aimed to exploring the fresh-keeping effect of chlorine dioxide onCuihong plum, and then providing a new way for postharvest fresh-keeping technology of Prunus. Methods: Cuihong plum fruits were treated with different concentrations of chlorine dioxide (20, 40, 60 mg/L) and then refrigerated at (1.0±0.5) ℃. Results: Compared with the control group, the group treated with 40 mg/L chlorine dioxide significantly reduced the decay rate, respiratory intensity, polyphenol oxidase activity (P<0.05), and inhibited the color difference L^* value, hardness, soluble solids content, titratable acid content, V_C content, peroxidase activity, the total number of mold and yeast colonies of Prunus (P<0.05). Conclusion: Treatment with 40 mg/L chlorine dioxide has the best storage effect, which can delay the metabolic process of Cuihong plum and maintain good storage quality.

Flavonoid Constituents And Antioxidant Activities Of Crude Ethanolic Extracts From Different Tissues Of Premna Puberula Pamb. In Guizhou, Yang Ning-Xian, Yang Jiao, Wang Yan-Qiu, Zhang Ming-Sheng

Food and Machinery

Objective: To enrich natural sources of antioxidants. Methods: The scavenging ability of DPPH and ABTS⁺ of extracts from Guizhou Premna puberula Pamb. was detected by ultrasonic extraction of total phenols and total flavonoids from roots, stems and leaves. Results: All the tissues had certain antioxidant activity and the antioxidant activity of leaves was the strongest, which was positively correlated with the contents of total phenols and total flavonoids. When the mass concentration was 0.1 mg/mL, the DPPH radical scavenging rate of leaves could reach 82.5%, which was close to the total reducing power of VC. Conclusion: It indicated that …

Optimization Of Polysaccharide Extraction Process From Aralia Elata（Miq.） Seem By Complex Enzymes And The Research Of Its Antioxidant Activity, Zhang Gui-Juan, Chen Ji-Ling, Tao Hui-Zhu

Food and Machinery

Objective: This study aimed to optimization of polysaccharide extraction process from Aralia elata (Miq.) Seem by complex enzymes and research of its antioxidant activity. Methods: The cellulase, pectinase and papain were combined as the compound enzymes and used to extract the polysaccharide from A. elata (AEPS). Enzymolysis temperature, enzymolysis duration, and solid-liquid ratio as the independent variables, a three-level-three-factor scheme was designed using the Box-Behnken method. After that, the antioxidant activity of AEPS was determined by the free radical scavenging ability experiments. Results: The optimal process conditions for the extraction process of AEPS using complex enzymes were as follows: enzymolysis …

Tomato Dry Peeling Technology Based On Catalytic Infrared, Liu Ying, Qu Wen-Juan, Luo Sheng-Tong, Ma Hai-Le, Pan Zhong-Li, Jiang Qun-Hui

Food and Machinery

Objective: An efficient and green method of peeling tomatoes was explored. Methods: A dry peeling method of tomatoes was carried out based on catalytic infrared equipment, and the effects of technological parameters on peeling performance and pulp quality of tomatoes were investigated and compared with traditional peeling methods of hot water and lye. Results: Infrared heating distance, temperature and time all had significant effects on the peelability and pulp quality of tomatoes. The optimum technological parameters of tomato dry peeling based on catalytic infrared was infrared heating temperature of 450 ℃, distance of 25 cm and time of 5 min. …

Preparation And Process Optimization Of Coconut Oil-Based Bile Salt Liposomes, Shi Zhen-Zhen, Bai Xin-Peng, Yue Yi-Jing

Food and Machinery

Objective: this study focuses on improving the water solubility of coconut oil and expand its application in food industry. Methods: According to the characteristics of the solubility of coconut oil, soybean lecithin - deoxycholate was used as shell material with coconut-oil core to establish coconut-oil bile salt liposomes by thin film dispersion method. Response surface optimization (RSM) was carried out according to the encapsulation rate, and the effects of several factors, including soybean lecithin/deoxycholate ratio, PBS/ anhydrous ethanol ratio, sample fat ratio, ultrasonic time, hydration time and hydration temperature, on the encapsulation rate were investigated. Results: The results showed that …

Review On Separation, Purification And Functional Activity Of Plant Tannin, Huang Da-Rong, Zhang Ya-Zhen, Zheng Bai-Tao, Du Bing, Li Pan

Food and Machinery

The types and applications of botanical tannin was reviewed. Moreover, the purification process and functional activity of tannin were summarized, and the relative mechanism was preliminarily introduced. The research direction in the future was also prospected.