Digital Commons Network^™

Mitophagy In Depression: Pathophysiology And Treatment Targets, Ashutosh Tripathi, Giselli Scaini, Tatiana Barichello, João Quevedo, Anilkumar Pillai

Faculty, Staff and Student Publications

Mitochondria, the 'powerhouse' of eukaryotic cells, play a key role in cellular homeostasis. However, defective mitochondria increase mitochondrial ROS (mtROS) production and cell-free mitochondrial DNA (mtDNA) release, leading to increased inflammation. Mitophagy is a vital pathway, which selectively removes defective mitochondria through the process of autophagy. Thus, an impairment in the mitophagy pathway might trigger the gradual accumulation of defective mitochondria. Accumulating evidence suggest that inflammation and mitochondrial dysfunction are linked to the pathogenesis of depression. In this article, we have reviewed the role of impaired mitophagy as a contributing factor in depression pathophysiology. Further, we have discussed the potential …

Analysis Of Covid-19 Infection And Mortality Among Patients With Psychiatric Disorders, 2020, Antonio L Teixeira, Trudy Millard Krause, Lopita Ghosh, Lokesh Shahani, Rodrigo Machado-Vieira, Scott D Lane, Eric Boerwinkle, Jair C Soares

Faculty, Staff and Student Publications

IMPORTANCE: People with major psychiatric disorders are more likely to have comorbidities associated with worse outcomes of COVID-19. This fact alone could determine greater vulnerability of people with major psychiatric disorders to COVID-19.

OBJECTIVE: To assess the odds of testing positive for and mortality from COVID-19 among and between patients with schizophrenia, mood disorders, anxiety disorders and a reference group in a large national database.

DESIGN, SETTING, AND PARTICIPANTS: This cross-sectional study used an electronic health record data set aggregated from many national sources in the United States and licensed from Optum with current and historical data on patients tested …

Hop2 Interacts With The Transcription Factor Cebpα And Suppresses Adipocyte Differentiation, Tonghui Lin, Yang Zhang, Tingting Zhang, Rita A Steckler, Xiangli Yang

Faculty, Staff and Student Publications

CCAAT enhancer binding protein (CEBP) transcription factors (TFs) are known to promote adipocyte differentiation; however, suppressors of CEBP TFs have not been reported thus far. Here, we find that homologous chromosome pairing protein 2 (Hop2) functions as an inhibitor for the TF CEBPα. We found that Hop2 mRNA is highly and specifically expressed in adipose tissue, and that ectopic Hop2 expression suppresses reporter activity induced by CEBP as revealed by DNA transfection. Recombinant and ectopically expressed Hop2 was shown to interact with CEBPα in pull-down and coimmunoprecipitation assays, and interaction between endogenous Hop2 and CEBPα was observed in the nuclei …

Positron Emission Tomography In The Covid-19 Pandemic Era, Chentao Jin, Xiaoyun Luo, Shufang Qian, Kai Zhang, Yuanxue Gao, Rui Zhou, Peili Cen, Zhoujiao Xu, Hong Zhang, Mei Tian

Faculty, Staff and Student Publications

Coronavirus disease 2019 (COVID-19) has become a major public health problem worldwide since its outbreak in 2019. Currently, the spread of COVID-19 is far from over, and various complications have roused increasing awareness of the public, calling for novel techniques to aid at diagnosis and treatment. Based on the principle of molecular imaging, positron emission tomography (PET) is expected to offer pathophysiological alternations of COVID-19 in the molecular/cellular perspectives and facilitate the clinical management of patients. A number of PET-related cases and research have been reported on COVID-19 over the past one year. This article reviews the current studies of …

Associations Between Genetic Variants In The Vitamin D Metabolism Pathway And Severity Of Covid-19 Among Uae Residents, Fatme Al-Anouti, Mira Mousa, Spyridon N. Karras, William B. Grant, Zainab Alhalwachi, Laila Abdel-Wareth, Maimunah Uddin, Nawal Alkaabi, Guan K. Tay, Bassam Mahboub, Habiba Alsafar

All Works

Vitamin D has many effects on cells in the immune system. Many studies have linked low vitamin D status with severity of COVID-19. Genetic variants involved in vitamin D metabolism have been implicated as potential risk factors for severe COVID-19 outcomes. This study investigated how genetic variations in humans affected the clinical presentation of COVID-19. In total, 646 patients with SARS-CoV-2 infection were divided into two groups: noncritical COVID-19 (n = 453; 70.12%) and a critical group (n = 193; 29.87%). Genotype data on the GC, NADSYN1, VDR, and CYP2R1 genes along with data on serum 25-hydroxyvitamin D levels were …

Citalopram For Treatment Of Cocaine Use Disorder: A Bayesian Drop-The-Loser Randomized Clinical Trial, Robert Suchting, Charles E Green, Constanza De Dios, Jessica Vincent, F Gerard Moeller, Scott D Lane, Joy M Schmitz

Faculty, Staff and Student Publications

BACKGROUND: Medication development research for cocaine use disorder (CUD) has been a longstanding goal in addiction research, but has not resulted in an FDA-approved treatment. Rising cocaine use rates underscore the need for efficient adaptive designs. This study compared differences between two doses of the selective serotonin reuptake inhibitor (SSRI) citalopram (versus placebo) on duration of cocaine abstinence and applied adaptive decision rules to select the 'best efficacy' dose.

METHODS: A double-blind, placebo-controlled, randomized Bayesian drop-the-loser (DTL) trial with three arms compared placebo to citalopram 20 mg and 40 mg. Adults (N = 107) with CUD attended thrice-weekly clinic visits …

Revisiting The Controversy: The Role Of Fungi In Chronic Rhinosinusitis, Matthew A Tyler, Kent Lam, Michael J Marino, William C Yao, Isaac Schmale, Martin J Citardi, Amber U Luong

Faculty, Staff and Student Publications

In the last two decades, the development of culture-independent genomic techniques has facilitated an increased appreciation of the microbiota-immunity interactions and their role in a multitude of chronic inflammatory diseases such as chronic rhinosinusitis (CRS), asthma, inflammatory bowel disease and dermatitis. While the pathologic role of bacteria in chronic inflammatory diseases is generally accepted, the understanding of the role of fungi remains controversial.

Chronic rhinosinusitis, specifically the phenotype linked to nasal polyps, represents a spectrum of chronic inflammatory diseases typically characterized by a Type 2 immune response. Studies on the microbiota within sinus cavities from healthy and diseased patients have …

Diagnostics To Support Elimination Of Lymphatic Filariasis-Development Of Two Target Product Profiles, Kimberly Y. Won, Katherine Gass, Marco Biamonte, Daniel Argaw Dagne, Camilla Ducker, Christopher Hanna, Achim Hoerauf, Patrick J. Lammie, Sammy M. Njenga, Rahmah Noordin, Kapa D. Ramaiah, Reda Ramzy, Ronaldo G.Carvalho Scholte, Anthony W. Solomon, Ashley A. Souza, Jordan Tappero, Emily Toubali, Gary J. Weil, Steven A. Williams, Jonathan D. King

Biological Sciences: Faculty Publications

As lymphatic filariasis (LF) programs move closer to established targets for validation elimination of LF as a public health problem, diagnostic tools capable of supporting the needs of the programs are critical for success. Known limitations of existing diagnostic tools make it challenging to have confidence that program endpoints have been achieved. In 2019, the World Health Organization (WHO) established a Diagnostic Technical Advisory Group (DTAG) for Neglected Tropical Diseases tasked with prioritizing diagnostic needs including defining use-cases and target product profiles (TPPs) for needed tools. Subsequently, disease-specific DTAG subgroups, including one focused on LF, were established to develop TPPs …

Vaccine Clinical Trials And Data Infrastructure, Ana Santos Rutschman

Faculty Publications

We find ourselves at a momentous turn in the history of vaccines. The COVID-19 pandemic triggered a quasi-global vaccine race that not only compressed vaccine research and development (R&D) timelines, but also paved the way for the administration of a new type of vaccine technology – mRNA vaccines, which work in substantially different ways from the vaccines in use before the pandemic.

While the process of bringing emerging COVID-19 vaccines to market has taken place in an unusually short timeframe, it was largely predicated on the same scientific and regulatory processes that govern the development, approval and deployment of new …

The Influence Of Maternal Glucocorticoids On Offspring Phenotype In High-And Low-Risk Environments, Kirsty J. Macleod, Tracy Langkilde, Cameron P. Venable, David C. Ensminger, Michael J. Sheriff

Faculty Research, Scholarly, and Creative Activity

Elevated maternal glucocorticoid levels during gestation can lead to phenotypic changes in offspring via maternal effects. Although such effects have traditionally been considered maladaptive, maternally derived glucocorticoids may adaptively prepare offspring for their future environment depending upon the correlation between maternal and offspring environments. Nevertheless, relatively few studies test the effects of prenatal glucocorticoid exposure across multiple environments. We tested the potential for ecologically relevant increases in maternal glucocorticoids in the eastern fence lizard (Sceloporus undulatus) to induce adaptive phenotypic changes in offspring exposed to high or low densities of an invasive fire ant predator. Maternal treatment had limited effects …

Informing Wetland Management With Waterfowl Movement And Sanctuary Use Responses To Human-Induced Disturbance, Fiona Mcduie, Austen A. Lorenz, Robert C. Klinger, Cory T. Overton, Cliff L. Feldheim, Joshua T. Ackerman, Michael L. Casazza

Faculty Research, Scholarly, and Creative Activity

Long-term environmental management to prevent waterfowl population declines is informed by ecology, movement behavior and habitat use patterns. Extrinsic factors, such as human-induced disturbance, can cause behavioral changes which may influence movement and resource needs, driving variation that affects management efficacy. To better understand the relationship between human-based disturbance and animal movement and habitat use, and their potential effects on management, we GPS tracked 15 dabbling ducks in California over ~4-weeks before, during and after the start of a recreational hunting season in October/November 2018. We recorded locations at 2-min intervals across three separate 24-h tracking phases: Phase 1) two …

Reduced Toxicity Of Centruroides Vittatus (Say, 1821) May Result From Lowered Sodium Β Toxin Gene Expression And Toxin Protein Production, Aimee Bowman, Chloe Fitzgerald, Jeff Pummill, Douglas D. Rhoads, Tsunemi Yamashita

Biological Sciences Faculty Publications and Presentations

Body tissue and venom glands from an eastern population of the scorpion Centruroides vittatus (Say, 1821) were homogenized and molecular constituents removed to characterize putative sodium beta toxin gene diversity, RT-qPCR, transcriptomic, and proteomic variation. We cloned sodium beta toxins from genomic DNA, conducted RT-qPCR experiments with seven sodium beta toxin variants, performed venom gland tissue RNA-seq, and isolated venom proteins for mass spectrophotometry. We identified >70 putative novel sodium beta toxin genes, 111 toxin gene transcripts, 24 different toxin proteins, and quantified sodium beta toxin gene expression variation among individuals and between sexes. Our analyses contribute to the growing …

Nine-Banded Armadillo (Dasypus Novemcinctus) Activity Patterns Are Influenced By Human Activity, Brett A. Degregorio, Connor Gale, Ellery V. Lassiter, Andrhea Darleen Massey, Caleb P. Roberts, John T. Veon

Biological Sciences Faculty Publications and Presentations

As the human footprint upon the landscape expands, wildlife seeking to avoid human contact are losing the option of altering their spatial distribution and instead are shifting their daily activity patterns to be active at different times than humans. In this study, we used game cameras to evaluate how human development and activity were related to the daily activity patterns of the nine-banded armadillo (Dasypus novemcinctus) along an urban to rural gradient in Arkansas, USA during the winter of 2020–2021. We found that armadillos had substantial behavioral plasticity in regard to the timing of their activity patterns; >95% of …

Lanthorn, Vol. 56, No. 11, November 1, 2021, Grand Valley State University

Volume 56, August 2, 2021 - April 18, 2022

Lanthorn is Grand Valley State's student newspaper, published from 1968 to the present.

Algae Sympoiesis In Performance: Rendering-With Nonhuman Ecologies, Sarah Blissett

School of Art, Design and Architecture

This article explores an ecodramaturgical approach to performance-making and research with algae. The first part considers the notion of ‘algae rendering’ as a methodological tool for theorising algae ecological relations which highlights links between representations of algae and their material effects. The second part considers how my embodied encounters with cyanobacteria algae, in the form of lichen, inspire new modes of working with algae in creative practice that explore how algae agencies ‘render’ bodies and environments. I also draw on an artistic case study by The Harrissons (1971) to illustrate principles of what I consider examples of ‘algae rendering’ in …

Topological Analysis Of The Carbon-Concentrating Cetch Cycle And A Photorespiratory Bypass Reveals Boosted Co2-Sequestration By Plants, Özge Osmanoglu, Mariam Khaled Alseiari, Hasa Abduljaleel Alkhoori, Shabana Shams, Elena Bencurova, Thomas Dandekar, Muhammad Naseem

All Works

Synthetically designed alternative photorespiratory pathways increase the biomass of tobacco and rice plants. Likewise, some in planta–tested synthetic carbon-concentrating cycles (CCCs) hold promise to increase plant biomass while diminishing atmospheric carbon dioxide burden. Taking these individual contributions into account, we hypothesize that the integration of bypasses and CCCs will further increase plant productivity. To test this in silico, we reconstructed a metabolic model by integrating photorespiration and photosynthesis with the synthetically designed alternative pathway 3 (AP3) enzymes and transporters. We calculated fluxes of the native plant system and those of AP3 combined with the inhibition of the glycolate/glycerate transporter by …

Building Legal Datasets, Jerrold Soh

Research Collection Yong Pung How School Of Law

Data-centric AI calls for better, not just bigger, datasets. As data protection laws with extra-territorial reach proliferate worldwide, ensuring datasets are legal is an increasingly crucial yet overlooked component of “better”. To help dataset builders become more willing and able to navigate this complex legal space, this paper reviews key legal obligations surrounding ML datasets, examines the practical impact of data laws on ML pipelines, and offers a framework for building legal datasets.

Simultaneous Brain And Intramedullary Spinal Abscesses In A Patient With Streptococcus Intermedius Infection, David P. Friedman, Joseph Desimone Jr, Aaron Christensen, Matthew Pettengill

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

A male patient in his thirties presented to the hospital with meningismus and altered mental status. Cerebrospinal fluid (CSF) analysis confirmed meningitis, but no microorganisms were identified. Head imaging (MRI) revealed multiple brain abscesses. Surgically-collected brain specimens revealed Gram-positive cocci and subsequently grew Streptococcus intermedius. MRI of the thoracic spine subsequently also identified an intramedullary spinal abscess. While brain abscesses and spinal abscesses caused by S. intermedius have been previously reported, the extent of disseminated disease in the patient was noteworthy.

Significance Of Open Science Approaches Towards The Fight Against Covid-19 Pandemic, Rajkumari Sofia Devi, Gyanajeet Yumnam, Ibohal Singh Ch

Library Philosophy and Practice (e-journal)

The Open science approach is based on collaborative knowledge generation, and it makes accessible scientific development to the public domain through networks using advanced technologies and collaborative tools. COVID-19 pandemic put the global scientific community from different disciplines to contribute their disciplinary perspectives and research approach to address the complex issues. This pandemic has witnessed a changing scientific culture among the scientists and scholarly publishers towards a more open science approach through sharing scientific data or statistics, adoption of quick review and more accessibility by lifting paywall by journal publishers to fight the pandemic more collaboratively. The present study aims …

Higher Habitual Flavonoid Intakes Are Associated With A Lower Incidence Of Diabetes, Nicola P. Bondonno, Frederik Dalgaard, Kevin Murray, Raymond J. Davey, Catherine P. Bondonno, Aedin Cassidy, Joshua R. Lewis, Cecilie Kyrø, Gunnar Gislason, Augustin Scalbert, Anne Tjønneland, Jonathan M. Hodgson

Research outputs 2014 to 2021

Background:

Higher flavonoid intakes are hypothesized to confer protection against type 2 diabetes mellitus.

Objectives:

We aimed to 1) investigate associations between flavonoid intakes and diabetes, 2) examine the mediating impact of body fat, and 3) identify subpopulations that may receive the greatest benefit from higher flavonoid intakes in participants of the Danish Diet, Cancer, and Health Study followed up for 23 y.

Methods:

Cross-sectional associations between baseline flavonoid intake, estimated using FFQs and the Phenol Explorer database, and body fat, estimated by bioelectrical impedance, were assessed using multivariable-Adjusted linear regression models. Nonlinear associations between flavonoid intake and incident diabetes …

Silver Bow Creek/Butte Area Npl Site Butte Priority Soils Operable Unit, Atlantic Richfield Company

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.

The Effect Of Maternal Traumatic Life Events And Social Support On Maternal-Child Interaction At Child Age 3 Years, Sandra I. Madubuonwu

Theses and Dissertations (ETD)

Introduction: Becoming a parent is a very important role and responsibility in people’s lives and knowing the role traumatic life events (TLEs) may play on a mother-child relationship is a very important area to explore. TLEs are known to have adverse effect on individuals; however, little is known regarding the effect of maternal TLEs on maternal-child interaction. Social support has been known to positively affect the overall wellbeing of individuals, but little is known about the effect of social support on mothers who experienced TLEs and interaction with their children thus the need for this study. This study examines the …

The Molecular Mechanisms Of Estrogen Receptor Α On Two Single Nucleotide Polymorphisms To Regulate Wnt Signaling In Osteoblasts, Sarocha Suthon

Theses and Dissertations (ETD)

Osteoporosis is the most common bone metabolic disorder, affecting over 200 million people globally. It is characterized by bone mass depletion and microarchitectural deterioration, leading to bone fragility and susceptibility to bone fracture. Genetic factors, estrogen deficiency, and dysregulation of the WNT signaling pathway contribute to the development of this disease. Genome-wide association studies have predicted that the single nucleotide polymorphisms (SNPs) rs2887571 and rs9921222 associate with low bone mass, but the mechanism of these SNPs has remained unknown. Analysis of osteoblasts from 112 different joint replacement patients reveals that the genotype of rs2887571 correlates with WNT5B expression, and the …

Advancing Rna Virus Discovery And Biology With Whole Genome Sequencing, Mariah Katherine Taylor

Theses and Dissertations (ETD)

Two RNA virus families that pose a threat to human and animal health are Hantaviridae and Coronaviridae. These RNA viruses which originate in wildlife continue and will continue to cause disease, and hence, it is critical that scientific research define the mechanisms as to how these viruses spillover and adapt to new hosts to become endemic. One gap in our ability to define these mechanisms is the lack of whole genome sequences for many of these viruses. To address this specific gap, I developed a versatile amplicon-based whole-genome sequencing (WGS) approach to identify viral genomes of hantaviruses and severe acute …

Role Of Smad2 And Smad3 On Adipose Tissue Development And Function, Roshan Kumari

Theses and Dissertations (ETD)

Introduction: Obesity and its associated metabolic syndrome are major medical problems worldwide including United States. Adipose tissue is the primary site of energy storage, playing important roles in health. Adipose tissue also has other critical functions, producing adipocytokines and contributing to normal nutrient metabolism, which in turn play important roles in satiety, inflammation, and total energy homeostasis. Activin A and activin B play important roles in maintaining body composition and energy homeostasis. This dissertation highlights the role of activin/SMADs signaling in adipose tissue development, function, and maintenance.

SMAD2/3 proteins are downstream mediators of transforming growth factor-β (TGFβ) family signaling, including …

Beluga Whale (Delphinapterus Leucas) Acoustic Foraging Behavior And Applications For Long Term Monitoring, Manuel Castellote, Aran Mooney, Russel Andrews, Stacy L. Deruiter

University Faculty Publications and Creative Works

Cook Inlet, Alaska, is home to an endangered and declining population of 279 belugas (Delphinapterus leucas). Recovery efforts highlight a paucity of basic ecological knowledge, impeding the correct assessment of threats and the development of recovery actions. In particular, information on diet and foraging habitat is very limited for this population. Passive acoustic monitoring has proven to be an efficient approach to monitor beluga distribution and seasonal occurrence. Identifying acoustic foraging behavior could help address the current gap in information on diet and foraging habitat. To address this conservation challenge, eight belugas from a comparative, healthy population in Bristol Bay, …

The Prospector, November 16, 2021, Utep Student Publications

The Prospector

Headline: UTEP Police Department Addresses Delayed Security Response in Forum

Comparison Of Culture With Antibiogram To Next-Generation Sequencing Using Bacterial Isolates And Formalin-Fixed, Paraffin-Embedded Gastric Biopsies, Kristina G Hulten, Robert M Genta, Ira N Kalfus, Yi Zhou, Hongjun Zhang, David Y Graham

Faculty, Staff and Students Publications

BACKGROUND & AIMS: The decline in Helicobacter pylori cure rates emphasizes the need for readily available methods to determine antimicrobial susceptibility. Our aim was to compare targeted next-generation sequencing (NGS) and culture-based H pylori susceptibility testing using clinical isolates and paired formalin-fixed, paraffin-embedded (FFPE) gastric biopsies.

METHODS: H pylori isolates and FFPE tissues were tested for susceptibility to amoxicillin, clarithromycin, metronidazole, levofloxacin, tetracycline, and rifabutin using agar dilution and NGS targeted to 23S rRNA, gyrA, 16S rRNA, pbp1, rpoB and rdxA. Agreement was quantified using κ statistics.

RESULTS: Paired comparisons included 170 isolates and FFPE tissue for amoxicillin, clarithromycin, metronidazole, …

Bone Turnover In Pregnancy, Measured By Urinary Ctx, Is Influenced By Vitamin D Supplementation And Is Associated With Maternal Bone Health: Findings From The Maternal Vitamin D Osteoporosis Study (Mavidos) Trial, Elizabeth M. Curtis, Camille Parsons, Kate Maslin, Stefania D'Angelo, Rebecca J. Moon, Sarah R. Crozier, Fatma Gossiel, Nicholas J. Bishop, Stephen H. Kennedy, Aris T. Papageorghiou, Robert Fraser, Saurabh V. Gandhi, Ann Prentice, Hazel M. Inskip, Keith M. Godfrey, Inez Schoenmakers, M. Kassim Javaid, Richard Eastell, Cyrus Cooper, Nicholas C. Harvey, N. K. Arden, A. Carr, M. Clynes, E. M. Dennison, M. Z. Mughal, S. J. Woolford

School of Nursing and Midwifery

Background The pattern of change in maternal bone turnover throughout pregnancy is poorly characterized. Objectives We investigated changes across pregnancy in a marker of maternal bone resorption, urinary C-terminal telopeptide of type I collagen (CTX), the influence of gestational vitamin D supplementation, and associations between CTX and maternal postnatal bone indices. Methods MAVIDOS (the Maternal Vitamin D Osteoporosis Study) is a randomized, double-blind, placebo-controlled trial of 1000 IU cholecalciferol/d compared with placebo from 14 weeks of gestation to birth. Maternal second-void urinary α- and β-CTX were measured (ELISA) at 14 and 34 weeks of gestation; DXA was performed within 2 …

Uk B.1.1.7 (Alpha) Variant Exhibits Increased Respiratory Replication And Shedding In Nonhuman Primates, Kyle Rosenke, F Feldmann, Atsushi Okumura, Frederick Hansen, Tsing Lee Tang-Huau, Kimberly Meade-White, Benjamin Kaza, Julie Callison, Matthew C. Lewis, Brian J. Smith, Patrick W. Hanley, Jamie Lovaglio, Michael A. Jarvis, Carl Shaia, H Feldmann

School of Biomedical Sciences

The continuing emergence of SARS-CoV-2 variants calls for regular assessment to identify differences in viral replication, shedding and associated disease. In this study, we compared African green monkeys infected intranasally with either the UK B.1.1.7 (Alpha) variant or its contemporary D614G progenitor. Both variants caused mild respiratory disease with no significant differences in clinical presentation. Significantly higher levels of viral RNA and infectious virus were found in upper and lower respiratory tract samples and tissues from B.1.1.7 infected animals. Interestingly, D614G infected animals showed significantly higher levels of viral RNA and infectious virus in rectal swabs and gastrointestinal tissues. Our …