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2021

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Advances In Forage Legumes: Shrub Legumes, M. H. Shelton Nov 2021

Advances In Forage Legumes: Shrub Legumes, M. H. Shelton

IGC Proceedings (1977-2023)

Forage tree legumes benefit agriculture through their multi-purpose contributions to livestock feeding, to productivity of farming systems, and to protection of the environment.

Of the several hundred forage tree legume species regarded as having potential for forage only c. twenty are in common use. These are listed together with their agroecological range and tolerance of adverse environments including acid soils, cool temperatures, low rainfall, poor drainage and high salinity. The value of many species to ruminants is limited due to low nutritive value arising from low protein availability and low acceptability caused principally by high condensed tannin content. Only 10 …


Alfalfa, J. H. Bouton Nov 2021

Alfalfa, J. H. Bouton

IGC Proceedings (1977-2023)

Cultivated alfalfa or lucerne (Medicago sativa spp. sativa) is the most important forage legume in the world with approximately 32 million hectares cultivated mainly in the temperate regions of both the northern and southern hemispheres. Intensive research has been conducted on the management and physiology of the crop and a major seed industry has developed world wide. Since insect and disease pests are numerous in alfalfa, development of cultivars with the proper fall dormancy and a broad genetic base for pest resistance has been pursued in order to provide increased adaptation, persistence, and yield. There has been a …


Carbon And Nitrogen Isotopic Discrimination In Alfalfa For High Yield And Quality Forage, W. S. Oliveira, P. P. A. Oliveira, M. Corsi, S. M. Tsai Nov 2021

Carbon And Nitrogen Isotopic Discrimination In Alfalfa For High Yield And Quality Forage, W. S. Oliveira, P. P. A. Oliveira, M. Corsi, S. M. Tsai

IGC Proceedings (1977-2023)

Biological nitrogen fixation and assimilation of CO2 can be indirectly monitored in alfalfa (Medicago sativa L.) through the variation of the stable N and C isotopes in the plant (Δ values). Such measurements result in a simple, non-destructive and reliable method for identification of alfalfa cultivars of high yield and nutritional quality. A program for selection of 12 alfalfa cultivars under field and controlled conditions (lysimeters) included determination of biomass and N accumulation. Under controlled conditions yields ranged from 1.77 to 2.47 ton/ha/month (Pioneer 5312 and WL-605) and N2-fixed varied from 77.43 to 84.01% for total N (Brazilian …


Power Reclaimed And Given: Female Passivity And Agency In Harris, Brand, And Smith, Annika Northrop Nov 2021

Power Reclaimed And Given: Female Passivity And Agency In Harris, Brand, And Smith, Annika Northrop

Journal of Interdisciplinary Undergraduate Research

The paper “Power Reclaimed and Given: Female Passivity and Agency in Harris, Brand, and Smith” explores representations of women in art in Claire Harris’ “After Image,” Dionne Brand’s “Blues Spiritual for Mammy Prater,” and Ali Smith’s How to be both. Harris’ poem depicts a woman, who is the subject of a male photographer’s photos and who has been objectified and relegated to passivity by the photographer, in the active process of reclaiming the power to create her own identity. Brand’s poem shows Mammy Prater as a woman who, although formerly subjugated and abused, has already reclaimed that power; in …


Effects Of Freshwater Input On Nutrient Loading, Toxic Cyanobacteria Bloom Development And Cyanotoxin Production In Lake Pontchartrain Estuary, Louisiana, Callie L. Snow Nov 2021

Effects Of Freshwater Input On Nutrient Loading, Toxic Cyanobacteria Bloom Development And Cyanotoxin Production In Lake Pontchartrain Estuary, Louisiana, Callie L. Snow

LSU Master's Theses

Estuaries in the northern Gulf of Mexico (GoM) are undergoing profound alterations due to changes in climate and implementation of coastal restoration activities and these may lead to the increasing frequency of harmful algal blooms (HABs). The introduction of large volumes of nutrient-rich, fresh Mississippi River water into nutrient-poor Lake Pontchartrain Estuary is known to substantially change the chemistry and ecology of the estuary, and warm temperatures paired with the persistent nutrient loading into the estuary supports the proliferation of potentially toxic cyanobacteria blooms (CyanoHABs). Throughout 2019, a long-term opening of the Bonnet Carré Spillway (BCS), initially from February to …


Hemp Microgreen Mineral Content, Cannabinoids, Total Phenolics, And Antioxidants, Cheston T. Schayot Nov 2021

Hemp Microgreen Mineral Content, Cannabinoids, Total Phenolics, And Antioxidants, Cheston T. Schayot

LSU Master's Theses

Hemp (Cannabis sativa) L. microgreens were grown to 7 and 12 days with and without supplemental lighting and/or fertilizer. The principal macronutrient found in hemp microgreens was nitrogen (N), followed by potassium (K), phosphorus (P), magnesium (Mg), calcium (Ca), and sulfur (S). The principal micronutrient found in hemp microgreens was iron (Fe), followed by manganese (Mn), zinc (Zn), boron (B), and copper (Cu). Hemp microgreens were similar to other commonly grown microgreen species in minerals except for P and Mg, where they could potentially be an excellent source, and Zn, where they fell below previously reported amounts. The …


Response Of Roseau Cane (Phragmites Australis) To Two Biotic Stresses: Hyalopterus Pruni And Bipolaris Yamadae, Heather E. Cizek Nov 2021

Response Of Roseau Cane (Phragmites Australis) To Two Biotic Stresses: Hyalopterus Pruni And Bipolaris Yamadae, Heather E. Cizek

LSU Master's Theses

Roseau cane (Phragmites australis) is considered an invasive plant because of its ability to replace native plant species. However, in Louisiana it plays an important role protecting coastal infrastructure and being part of the marsh ecosystem in the lower Mississippi River Delta. In recent years, Roseau cane has been affected by a die-off, a problem that has also been reported in some European countries. Possible biotic and abiotic factors that have been associated with the die-off include scale insects, climate change, pollution, salinity levels, and pathogens.

In this research, the individual and combined effect of a foliar disease …


3-Dimensional Structural Determination Of Epidermal Growth Factor-Like Repeat 27 From Mouse Notch1 Using Nuclear Magnetic Resonance Spectroscopy, Justin Grennell Nov 2021

3-Dimensional Structural Determination Of Epidermal Growth Factor-Like Repeat 27 From Mouse Notch1 Using Nuclear Magnetic Resonance Spectroscopy, Justin Grennell

LSU Doctoral Dissertations

NOTCH1 has been associated with a variety of human ailments, such as cancer and heart disease. NOTCH1 is a transmembrane protein that contains 36 epidermal growth factor-like (EGF) repeats, which facilitate cell signaling by binding proteins on neighboring cells. Each repeat contains about 40 amino acids and has to be folded in a specific manner to function properly. The amino acid sequence, carbohydrate pattern, and calcium-binding sites of EGF-like repeats vary, but the pattern along the 36-repeat chain is conserved. The Abruptex region (EGF24-29) of NOTCH1 is a highly glycosylated (having undergone sugar addition) and flexible region, which gained its …


Can Open-Mindedness Be Primed? An Investigation Into Creativity, Openness To Experience, And Open-Mindedness Among College Students With Implications For Counselors, Alex Gallimore Nov 2021

Can Open-Mindedness Be Primed? An Investigation Into Creativity, Openness To Experience, And Open-Mindedness Among College Students With Implications For Counselors, Alex Gallimore

Journal of Interdisciplinary Graduate Research

With an ever-diversifying population, open-mindedness regarding an individual’s values and beliefs is important, especially for counselors. This study hypothesized that creativity could be a means to increase one’s open-mindedness. Utilizing a quasi-experimental design, this study examined the impact of a creative task on participants’ open-mindedness. Additionally, a correlation was sought between open-mindedness and the Big Five personality trait of Openness to Experience as well as a comparison between participants studying for careers in the helping professions (counselors, psychologists, and social workers) and all other participants. Two hundred and forty-four students at a small, private university participated. Results found no significant …


Episode 13: Elena Cyrus: Expertise On Infectious Diseases And Vaccines, University Of Central Florida Nov 2021

Episode 13: Elena Cyrus: Expertise On Infectious Diseases And Vaccines, University Of Central Florida

Knights Do That Podcast Transcripts

In episode 13 of Knights Do That, we speak with Elena Cyrus, an infectious disease epidemiologist at UCF’s College of Medicine. She discusses her expertise and research in infectious diseases, public health and COVID-19 vaccines.

Produced by UCF, the podcast highlights students, faculty, staff, administrators and alumni who do incredible things on campus, in the community and around the globe.


Insight - Fall 2021, Dugoni School Of Dentistry Nov 2021

Insight - Fall 2021, Dugoni School Of Dentistry

Insight

Spotlighting the work of people at the Dugoni School, including clinical and biomedical research, the scholarship of teaching and learning, improvement of the health care system, professional partnerships that advance the field and more.


Which Outcomes Are Most Important To Measure In Patients With Covid-19 And How And When Should These Be Measured? Development Of An International Standard Set Of Outcomes Measures For Clinical Use In Patients With Covid-19: A Report Of The International Consortium For Health Outcomes Measurement (Ichom) Covid-19 Working Group., William H Seligman, Luz Fialho, Nick Sillett, Christina Nielsen, Farhala M Baloch, Philip Collis, Ingel K M Demedts, Marcelo P Fleck, Maiara A Floriani, Lucinda E K Gabriel, Joel J Gagnier, Anju Keetharuth, Ana Londral, Ingvar I L Ludwig, Carlos Lumbreras, Alejandro Moscoso Daza, Nasreen Muhammad, Gisele A Nader Bastos, Christine W Owen, John H. Powers, Anne-Marie Russell, Michaela K Smith, Tracy Y-P Wang, Evan K Wong, Douglas C Woodhouse, Eyal Zimlichman, Kees Brinkman Nov 2021

Which Outcomes Are Most Important To Measure In Patients With Covid-19 And How And When Should These Be Measured? Development Of An International Standard Set Of Outcomes Measures For Clinical Use In Patients With Covid-19: A Report Of The International Consortium For Health Outcomes Measurement (Ichom) Covid-19 Working Group., William H Seligman, Luz Fialho, Nick Sillett, Christina Nielsen, Farhala M Baloch, Philip Collis, Ingel K M Demedts, Marcelo P Fleck, Maiara A Floriani, Lucinda E K Gabriel, Joel J Gagnier, Anju Keetharuth, Ana Londral, Ingvar I L Ludwig, Carlos Lumbreras, Alejandro Moscoso Daza, Nasreen Muhammad, Gisele A Nader Bastos, Christine W Owen, John H. Powers, Anne-Marie Russell, Michaela K Smith, Tracy Y-P Wang, Evan K Wong, Douglas C Woodhouse, Eyal Zimlichman, Kees Brinkman

Medicine Faculty Publications

No abstract provided.


A 6-Cpg Validated Methylation Risk Score Model For Metabolic Syndrome: The Hypergen And Goldn Studies, Bertha A. Hidalgo, Bre Minniefield, Amit Patki, Rikki Tanner, Minoo Bagheri, Hemant K. Tiwari, Donna K. Arnett, Marguerite Ryan Irvin Nov 2021

A 6-Cpg Validated Methylation Risk Score Model For Metabolic Syndrome: The Hypergen And Goldn Studies, Bertha A. Hidalgo, Bre Minniefield, Amit Patki, Rikki Tanner, Minoo Bagheri, Hemant K. Tiwari, Donna K. Arnett, Marguerite Ryan Irvin

Epidemiology and Environmental Health Faculty Publications

There has been great interest in genetic risk prediction using risk scores in recent years, however, the utility of scores developed in European populations and later applied to non-European populations has not been successful. The goal of this study was to create a methylation risk score (MRS) for metabolic syndrome (MetS), demonstrating the utility of MRS across race groups using cross-sectional data from the Hypertension Genetic Epidemiology Network (HyperGEN, N = 614 African Americans (AA)) and the Genetics of Lipid Lowering Drugs and Diet Network (GOLDN, N = 995 European Americans (EA)). To demonstrate this, we first selected cytosine-guanine dinucleotides …


Predicting Thermal Responses Of An Arctic Lake To Whole-Lake Warming Manipulation, Qunhui Zhang, Jiming Jin, Phaedra Budy, Sarah E. Null, Xiaochun Wang, Casey A. Pennock Nov 2021

Predicting Thermal Responses Of An Arctic Lake To Whole-Lake Warming Manipulation, Qunhui Zhang, Jiming Jin, Phaedra Budy, Sarah E. Null, Xiaochun Wang, Casey A. Pennock

Watershed Sciences Faculty Publications

We investigated how lake thermal processes responded to whole lake warming manipulation in an arctic lake through observations and numerical modeling. The warming manipulation was conducted by artificially heating the epilimnion as a proxy for climate warming. We performed numerical modeling with an improved lake scheme based on the Community Land Model (CLM). We simulated a control run (CTL) without warming and a warming manipulation simulation (WARM). Results indicated WARM accurately captured observed temperatures where water stratification was extended in time, and water stability was strengthened. Two additional sensitivity tests with different warming onset dates and of the same warming …


Doomed To Fail: Ag-Gag Laws And The Canadian Charter, Samantha Lynne Skinner Nov 2021

Doomed To Fail: Ag-Gag Laws And The Canadian Charter, Samantha Lynne Skinner

LLM Theses

In late 2019, ag-gag laws began being introduced in Canada. Ag-gag laws are named for their intended effect of gagging activists from exposing the realities of the animal agriculture industry. Animal activists seek to gather and publicly disseminate information using means of bearing witness, undercover investigations, and civil disobedience. Ag-gag laws originated in the US in the 1990s, but saw a revival in the 2010s. In the US, animal law organizations such as the Animal Legal Defense Fund have been successfully challenging the constitutionality of ag-gag laws, with courts in six states finding ag-gag laws to violate the First Amendment …


Faculty Senate Executive Committee Agenda, November 15, 2021, Utah State University Nov 2021

Faculty Senate Executive Committee Agenda, November 15, 2021, Utah State University

Faculty Senate & Faculty Senate Executive Committee

  • Approval of Minutes – October 18, 2021
  • University Business
  • Education Policies Committee
  • Academic Freedom and Tenure Annual Report
  • Libraries Advisory Council Annual Report
  • Faculty Evaluation Committee Annual Report
  • USUSA Annual Report
  • Old Business
  • Code 401 Track Changes | Clean 1st Reading
  • Code 402 Track Changes | Clean 1st Reading


Relating Multivariate Shapes To Genescapes Using Phenotype-Biological Process Associations For Craniofacial Shape., Jose D Aponte, David C Katz, Daniela M Roth, Marta Vidal-García, Wei Liu, Fernando Andrade, Charles C Roseman, Stephen A Murray, James Cheverud, Daniel Graf, Ralph S Marcucio, Benedikt Hallgrímsson Nov 2021

Relating Multivariate Shapes To Genescapes Using Phenotype-Biological Process Associations For Craniofacial Shape., Jose D Aponte, David C Katz, Daniela M Roth, Marta Vidal-García, Wei Liu, Fernando Andrade, Charles C Roseman, Stephen A Murray, James Cheverud, Daniel Graf, Ralph S Marcucio, Benedikt Hallgrímsson

Faculty Research 2021

Realistic mappings of genes to morphology are inherently multivariate on both sides of the equation. The importance of coordinated gene effects on morphological phenotypes is clear from the intertwining of gene actions in signaling pathways, gene regulatory networks, and developmental processes underlying the development of shape and size. Yet, current approaches tend to focus on identifying and localizing the effects of individual genes and rarely leverage the information content of high-dimensional phenotypes. Here, we explicitly model the joint effects of biologically coherent collections of genes on a multivariate trait - craniofacial shape - in a sample of n = 1145 …


Contact Point Autumn 2021, Arthur A. Dugoni School Of Dentistry Nov 2021

Contact Point Autumn 2021, Arthur A. Dugoni School Of Dentistry

Contact Point

No abstract provided.


Relief And Reward Drinking Across Time In Community And Treatment-Seeking Samples, Julia E. Swan Nov 2021

Relief And Reward Drinking Across Time In Community And Treatment-Seeking Samples, Julia E. Swan

Psychology ETDs

Classifying people with alcohol use disorder into homogenous groups based on observed characteristics (i.e., phenotypes) could match individuals to specific treatments. The reward-relief phenotype classifies individuals based on whether they drink to enhance rewarding experiences and/or to relieve negative states. The current study is a secondary data analysis of a community sample of non-treatment seeking heavy drinkers (n = 189) and two randomized clinical trial samples of individuals with alcohol use disorder (n = 1726, 1383) that aimed to determine if the reward-relief phenotype is identified across samples, is stable over time, and predicts long-term alcohol consumption and consequences. We …


The Utah Statesman, November 15, 2021, Utah State University Nov 2021

The Utah Statesman, November 15, 2021, Utah State University

The Utah Statesman

Weekly student newspaper of Utah State University in Logan.


The Rider (2021-11-15), Verilu Infante Nov 2021

The Rider (2021-11-15), Verilu Infante

The Rider Archives

Vol.7 No.13


Beeutiful Partnership For Pollinators And Our Ecosystems, Cassie Hollasch Nov 2021

Beeutiful Partnership For Pollinators And Our Ecosystems, Cassie Hollasch

Sustainability Institute Blog

No abstract provided.


The Daily Gamecock, Traditions, November 2021, University Of South Carolina, Office Of Student Media Nov 2021

The Daily Gamecock, Traditions, November 2021, University Of South Carolina, Office Of Student Media

November

No abstract provided.


Audacious Hope: The Inauguration Of Vincent D. Rougeau, 2021, College Of The Holy Cross Nov 2021

Audacious Hope: The Inauguration Of Vincent D. Rougeau, 2021, College Of The Holy Cross

Holy Cross Magazine

Audacious Hope: The Inauguration of Vincent D. Rougeau
Relive the three-day celebration of the College's 33rd president and the events that surrounded his installation as Holy Cross' first lay leader. From faculty symposium to fireworks, dive into the photos and words that accompanied three historic days in October 2021.

From the Chair of the Board of Trustees
“The Character and Vision of Vincent D. Rougeau”
By Richard “Rick” Patterson ’80

“Global Engagement and Democratic Citizenship” Faculty Symposium Keynote Address
A transcript of the speech presented by Martha Minow, 300th Anniversary University Professor, Harvard University

What is Holy Cross’ Role, Responsibility …


Protocol For Qualitative Systematic Review, Abigail Ekeigwe, Bethany S. Mcgowan, Kari Clase, Stephen Byrn, P. Shivanand, L. Parker Nov 2021

Protocol For Qualitative Systematic Review, Abigail Ekeigwe, Bethany S. Mcgowan, Kari Clase, Stephen Byrn, P. Shivanand, L. Parker

BIRS Technical Report Papers

No abstract provided.


Discovery Of Imidazole-Based Inhibitors Of Plasmodium Falciparum Cgmp-Dependent Protein Kinase, Rammohan R. Yadav Bheemanaboina, Mariana Laureano De Souza, Mariana Lozano Gonzalez, Shams Ul Mahmood, Tyler Eck, Tamara Kreiss, Samantha O. Aylor, Alison Roth, Patricia Lee, Brandon S. Pybus, Dennis J. Colussi, Wayne E. Childers, John Gordon, John J. Siekierka, Purnima Bhanot, David P. Rotella Nov 2021

Discovery Of Imidazole-Based Inhibitors Of Plasmodium Falciparum Cgmp-Dependent Protein Kinase, Rammohan R. Yadav Bheemanaboina, Mariana Laureano De Souza, Mariana Lozano Gonzalez, Shams Ul Mahmood, Tyler Eck, Tamara Kreiss, Samantha O. Aylor, Alison Roth, Patricia Lee, Brandon S. Pybus, Dennis J. Colussi, Wayne E. Childers, John Gordon, John J. Siekierka, Purnima Bhanot, David P. Rotella

Department of Chemistry and Biochemistry Faculty Scholarship and Creative Works

The discovery of new targets for the treatment of malaria, in particular those aimed at the pre-erythrocytic stage in the life cycle, advanced with the demonstration that orally administered inhibitors of Plasmodium falciparum cGMP-dependent protein kinase (PfPKG) could clear infection in a murine model. This enthusiasm was tempered by unsatisfactory safety and/or pharmacokinetic issues found with these chemotypes. To address the urgent need for new scaffolds, this paper presents initial structure–activity relationships in an imidazole scaffold at four positions, representative in vitro ADME, hERG characterization, and cell-based antiparasitic activity. This series of PfPKG inhibitors has good in vitro PfPKG potency, …


Comparative Phylogeography Across Multiple Scales: Small Mammals, Their Ecology, Pathogens, And Drivers Of Diversification, Schuyler W. Liphardt Nov 2021

Comparative Phylogeography Across Multiple Scales: Small Mammals, Their Ecology, Pathogens, And Drivers Of Diversification, Schuyler W. Liphardt

Biology ETDs

Comparative phylogeography has historically been defined as the study of how genetic variation of co-distributed species has been shaped by biogeographical history. This is a mature field of study out of which several techniques have been developed directed at identifying the role ecology and geography in diversification patterns across time. I employ the tools developed in classical comparative phylogeography across multiple taxonomic scales and across regions that historically have been understudied. My first two chapters study the complex history of host-switching, codiversification, and reassortment of hantaviruses in their mammal hosts across North America. By taking a host-centric comparative phylogeographic approach …


Peruvian Fur Seals As Archivists Of El Niño Southern Oscillation Effects, Mickie Rae Edwards, Susana Cardenas-Alayza, Michael Adkesson, Mya Daniels-Abdulahad, Amy Hirons Nov 2021

Peruvian Fur Seals As Archivists Of El Niño Southern Oscillation Effects, Mickie Rae Edwards, Susana Cardenas-Alayza, Michael Adkesson, Mya Daniels-Abdulahad, Amy Hirons

Marine & Environmental Sciences Faculty Articles

Peru’s coastal waters are characterized by significant environmental fluctuation due to periodic El Niño- La Niña- Southern Oscillation (ENSO) events. This variability results in ecosystem-wide food web changes which are reflected in the tissues of the Peruvian fur seal (Arctocephalus australis). Stable isotope ratios (δ13C and δ15N) in Peruvian fur seal vibrissae (whiskers) are used to infer temporal primary production and dietary variations in individuals. Sea surface temperature anomaly (SSTA) recordings from the Niño 1+2 Index region captured corresponding ENSO conditions. Fluctuations in δ15N values were correlated to SSTA records, indicating that …


Which Outcomes Are Most Important To Measure In Patients With Covid-19 And How And When Should These Be Measured? Development Of An International Standard Set Of Outcomes Measures For Clinical Use In Patients With Covid-19: A Report Of The International Consortium For Health Outcomes Measurement (Ichom) Covid-19 Working Group, William H. Seligman, Luz Fialho, Nick Sillett, Christina Nielsen, Farhala Mari Baloch, Philip Collis, Ingel K M. Demedts, Marcelo P. Fleck, Maiara A. Floriani, Nasreen Muhammad Nov 2021

Which Outcomes Are Most Important To Measure In Patients With Covid-19 And How And When Should These Be Measured? Development Of An International Standard Set Of Outcomes Measures For Clinical Use In Patients With Covid-19: A Report Of The International Consortium For Health Outcomes Measurement (Ichom) Covid-19 Working Group, William H. Seligman, Luz Fialho, Nick Sillett, Christina Nielsen, Farhala Mari Baloch, Philip Collis, Ingel K M. Demedts, Marcelo P. Fleck, Maiara A. Floriani, Nasreen Muhammad

Section of Cardiology

Objectives: The COVID-19 pandemic has resulted in widespread morbidity and mortality with the consequences expected to be felt for many years. Significant variation exists in the care even of similar patients with COVID-19, including treatment practices within and between institutions. Outcome measures vary among clinical trials on the same therapies. Understanding which therapies are of most value is not possible unless consensus can be reached on which outcomes are most important to measure. Furthermore, consensus on the most important outcomes may enable patients to monitor and track their care, and may help providers to improve the care they offer through …


Resource Allocation And Phenotypic Plasticity Of Simultaneous Hermaphroditic Turtle Barnacles (Chelonibia Testudinaria), Kevin C. Cash Nov 2021

Resource Allocation And Phenotypic Plasticity Of Simultaneous Hermaphroditic Turtle Barnacles (Chelonibia Testudinaria), Kevin C. Cash

All HCAS Student Capstones, Theses, and Dissertations

This research addresses the knowledge gap of phenotypic plasticity in a commonly found and important species of epizoic barnacle, Chelonibia testudinaria. Limited research has been published regarding how phenotypic expression is mediated the spatial distribution of barnacles on a mobile host. To investigate this potential relationship, barnacles were collected from the backs of turtles along the beaches of Fort Lauderdale Florida. These barnacles were assessed for various phenotypic traits as well as their corresponding spatial distribution on the turtle carapace. Barnacles were safely removed from the carapace using a chisel before their preservation in ethanol. Barnacles were then numbered …