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2021

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Cellular Responses Of Stem Cells Isolated From Human Exfoliated Deciduous Teeth Towards Different Concentration Of Calcium Ion, Thanika Phlinyos Jan 2021

Cellular Responses Of Stem Cells Isolated From Human Exfoliated Deciduous Teeth Towards Different Concentration Of Calcium Ion, Thanika Phlinyos

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objectives: The purpose of this study was to investigate the effect of Ca2+ on proliferation, osteogenic differentiation, and migration of stem cells from human exfoliated teeth (SHEDs) in vitro Materials and methods: SHEDs were seeded in culture media and osteogenic induction media containing 1.8-16.2 mM of Ca2+. SHEDs proliferation was determined using MTT assay and colony forming unit assay. Osteogenic differentiation was evaluated using mineralization assay and osteogenic marker gene expression and cell migration was evaluated using wound healing assay. Values were expressed as mean + S.D. Statistical analysis of MTT assay and wound healing assay were performed using two-way …

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Expression Of Vascular Endothelial Growth Factor Protein In Mrna-Transfected Human Periodontal Ligament Cells, Mana Naratippakorn Jan 2021

Expression Of Vascular Endothelial Growth Factor Protein In Mrna-Transfected Human Periodontal Ligament Cells, Mana Naratippakorn

Chulalongkorn University Theses and Dissertations (Chula ETD)

The complete regeneration of periodontal tissues following current periodontal therapy remain challenging and unpredictable. Nucleoside-modified messenger RNA (mRNA) technology can be a promising novel platform in regenerative medicine. The aims of this study were to evaluate whether pseudouridine modified mRNA encoding vascular endothelial growth factor (VEGF) could induce VEGF production in human periodontal ligament cells (PDLCs) and this translated protein function by promoting in vivo blood vessel formation using chorioallantoic membrane (CAM) assay. Isolated PDLCs from healthy periodontal tissue were transfected with modified mRNA encoding VEGF (VEGF mRNA) complexed with a transfecting agent, Lipofectamine 2000 (L2000) and L2000 alone (control). …

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Clinical Efficacy Of Silk Sericin Dressing With Collagen For Split-Thickness Skin Graft Donor Site Treatment, Apirujee Punjataewakupt Jan 2021

Clinical Efficacy Of Silk Sericin Dressing With Collagen For Split-Thickness Skin Graft Donor Site Treatment, Apirujee Punjataewakupt

Chulalongkorn University Theses and Dissertations (Chula ETD)

Sericin dressing containing collagen hydrolysate (SDC) was evaluated in this study, consisting of two phases of clinical trials. The clinical safety of SDC was determined using a patch test in 103 healthy volunteers (phase I). Most of the results (around 90%) in the SDC group were interpreted as negative and doubtful reactions, and the remaining results (around 10%) were weak reactions. After that, the clinical efficacy and safety of SDC were investigated in 21 patients with 30 split-thickness skin graft (STSG) donor sites (phase II). Each donor site was divided into two equal sites. SDC or the commercial dressing (Bactigras®) …

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Production Optimization Of Porcine Reproductive And Respiratory Syndrome (Prrs) Virus Nucleocapsid Protein In Nicotiana Benthamiana, Boonyasit Porngarm Jan 2021

Production Optimization Of Porcine Reproductive And Respiratory Syndrome (Prrs) Virus Nucleocapsid Protein In Nicotiana Benthamiana, Boonyasit Porngarm

Chulalongkorn University Theses and Dissertations (Chula ETD)

Porcine Reproductive and Respiratory Syndrome virus (PRRSV) is a considerable pathogen to occur disease in pigs and effect to economic on the swine industry around the world including Thailand. Routine observation, rapid diagnosis of PRRSV infection is essential for effective monitoring and disease control. The serological assays using highly conserved viral nucleocapsid (N) protein are widely used for the detection of antibodies to the PRRSV. Accordingly, this study was aimed to produce the N-protein of the PRRSV in the plant system by using Nicotiana benthamiana. The N-protein gene was designed into four constructs that contained with and without the plant …

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Antioxidant Activity Of Thunbergia Laurifolia Lindl. Ethanolic Extract In Keratinocytes, Buakotchaphan Jirabanjerdsiri Jan 2021

Antioxidant Activity Of Thunbergia Laurifolia Lindl. Ethanolic Extract In Keratinocytes, Buakotchaphan Jirabanjerdsiri

Chulalongkorn University Theses and Dissertations (Chula ETD)

Fine particulate matter (PM2.5) can cause skin damage through the induction of oxidative stress in epidermis. Antioxidants help counteract with cellular oxidant species and maintain cell homeostasis. This study aims to examine the protective effect of Thunbergia laurifolia lindl. ethanolic extract on PM2.5-mediated oxidative stress in epidermal keratinocytes. High-performance liquid chromatography with diode array detection was conducted for quantitative analysis of rosmarinic acid content in the extract. Cell viability was detected by the 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide assay. Intracellular ROS was monitored using 2’,7’-Dichlorodihydrofluorescein diacetate. Proteins related to p62-Keap1-Nrf2 signaling pathway were examined by western blot analysis. Expressions of Nrf2 and p62 …

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Antioxidant With Antimelanogenic Activities Of Sericin Hydrolysates Optimized By Response Surface Methodology In Human Melanin-Generating Mnt-1 Cells, Keerati Joyjamras Jan 2021

Antioxidant With Antimelanogenic Activities Of Sericin Hydrolysates Optimized By Response Surface Methodology In Human Melanin-Generating Mnt-1 Cells, Keerati Joyjamras

Chulalongkorn University Theses and Dissertations (Chula ETD)

Sericin, a protein presenting in the wastewater from silk industry, causes water pollution and ecological problem. To increase economic value to this waste product, the optimum enzymatic condition that could create sericin hydrolysates with high antioxidant and antimelanogenic capacities was generated through response surface methodology (RSM). Response surface plots demonstrate the major role of temperature on scavenging capacity of sericin hydrolysates assessed via DPPH, FRAP and ORAC assays. Alcalase®-hydrolyzed sericin consisted of sericin-related peptides in differing amounts and smaller sizes compared with unhydrolyzed sericin, as respectively demonstrated by peptidomic and SDS-PAGE analysis. Pre-incubation with 20 mg/mL sericin hydrolysates digested by …

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Development Of Plant-Produced Subunit Vaccine And Therapeutic Protein For Covid-19, Konlavat Siriwattananon Jan 2021

Development Of Plant-Produced Subunit Vaccine And Therapeutic Protein For Covid-19, Konlavat Siriwattananon

Chulalongkorn University Theses and Dissertations (Chula ETD)

Due to the rapid transmission of the Coronavirus Disease 2019 (COVID-19) causing serious public health problems and economic burden, the development of effective therapeutic interventions is urgently needed for controlling the ongoing pandemic disease. In this study, we have transiently produced recombinant SARS-CoV-2 RBD and human ACE2 fused with the Fc region of human IgG1 in Nicotiana benthamianain in order to use as a preventive subunit vaccine and therapeutic against SARS-CoV-2. The recombinant SARS-CoV-2 RBD-Fc subunit vaccine was formulated with different commercially available adjuvants including aluminium hydroxide gel (Alum), AddaVaxTM (MF59), monophosphoryl lipid A from Salmonella Minnesota R595 (mPLA-SM), and …

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Taxonomy And Biological Activities Of A Novel Saccharothrix Species From Soil Of Sichang Island, Md Abul Kalam Azad Jan 2021

Taxonomy And Biological Activities Of A Novel Saccharothrix Species From Soil Of Sichang Island, Md Abul Kalam Azad

Chulalongkorn University Theses and Dissertations (Chula ETD)

Actinobacteria are the sources of bioactive natural products. Rare actinobacteria are the less frequently isolated genera of actinobacteria. During an investigation of diversity of rare actinobacteria, actinobacteria isolate SC076T was isolated from a soil sample collected from Sichang Island, Chonburi Province, Thailand. The strain showed the highest 16S rRNA gene similarity to Saccharothrix australiensis DSM 43800T (98.58%) and Saccharothrix espanaensis DSM 44229T (98.58%). The zigzag morphology of the spore chain was observed on the aerial mycelia. meso-diaminopimelic acid was detected in the peptidoglycan. Whole-cell sugars contained rhamnose, ribose, mannose glucose and galactose. Polar lipids are phosphatidylethanolamine, hydroxyphosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol …

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Effect Of Hydroquinone 5-O-Cinnamoyl Ester Of Renieramycin M On The Suppression Of Lung Cancer Stem Cells, Nattamon Hongwiangchan Jan 2021

Effect Of Hydroquinone 5-O-Cinnamoyl Ester Of Renieramycin M On The Suppression Of Lung Cancer Stem Cells, Nattamon Hongwiangchan

Chulalongkorn University Theses and Dissertations (Chula ETD)

Cancer stem cells (CSCs) are distinct cancer populations with tumorigenic and self-renewal abilities. CSCs are drivers of cancer initiation, progression, therapeutic failure, and disease recurrence. Thereby, novel compounds targeting CSCs offer a promising way to control cancer. In this study, the hydroquinone 5-O-cinnamoyl ester of renieramycin M (CIN-RM) was demonstrated to suppress lung cancer CSCs. CIN-RM was toxic to lung cancer cells with a half-maximal inhibitory concentration around 15 µM. CIN-RM suppressed CSCs by inhibiting colony and tumor spheroid formation. In addition, the CSC population was isolated and treated and the CSCs were dispatched in response to CIN-RM within 24 …

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Α-Glucosidase Inhibitors From Dendrobium Delacourii, Dendrobium Gibsonii And Aerides Multiflora, May Thazin Thant Jan 2021

Α-Glucosidase Inhibitors From Dendrobium Delacourii, Dendrobium Gibsonii And Aerides Multiflora, May Thazin Thant

Chulalongkorn University Theses and Dissertations (Chula ETD)

In this study, three plants from the Orchidaceae family, i.e., Dendrobium delacourii, Dendrobium gibsonii, and Aerides multiflora, were investigated for their chemical constituents and α-glucosidase inhibitory activities. A total of thirty compounds were isolated and structurally characterized. Eleven known compounds were identified from Dendrobium delacourii, including hircinol, ephemeranthoquinone, densiflorol B, moscatin, 4,9-dimethoxy-2,5-phenanthrenediol, gigantol, batatasin III, lusianthridin, 4,4',7,7'-tetrahydroxy-2,2'-dimethoxy-9,9',10,10'-tetrahydro-1,1'-biphenanthrene, phoyunnanin E, and phoyunnanin C. Two new compounds, i.e., dihydrodengibsinin and dendrogibsol, were isolated from Dendrobium gibsonii, along with seven known compounds including ephemeranthol A, dengibsinin, nobilone, aloifol I, lusianthridin, denchrysan A, and 4-methoxy-9H-fluorene-2,5,9-triol. Four new compounds, i.e., aerimultins A-C and dihydrosinapyl dihydroferulate, …

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Lipase Inhibitors From Dendrobium Senile, Myat Pann Phyu Jan 2021

Lipase Inhibitors From Dendrobium Senile, Myat Pann Phyu

Chulalongkorn University Theses and Dissertations (Chula ETD)

Obesity is characterized as an abnormal lipid metabolism due the imbalance between the energy intake and output. Pancreatic lipase is secreted by the pancreas and plays an important role in the digestion and absorption of lipids in the small intestine. Inhibition of pancreatic lipase enzyme can reduce dietary fat absorption which will lead to loss of weight and finally result in hypocholesterolemia and hypoglycemia. From the whole plant of Dendrobium senile, a new phenanthrene derivative was isolated, together with seven known compounds, including 2,5,7-trihydroxy-4-methoxyphenanthrene (1), moscatin (2), 2,5-dihydroxy-4,9-dimethoxyphenanthrene (3), moscatilin (4), aloifol I (5), 4,4ʹ,8,8ʹ-tetramethoxy[1,1ʹ-biphenanthrene]-2,2ʹ,7,7ʹ-tetrol (6), 2,2ʹ,7,7ʹ-tetrahydroxy-4,4ʹ-dimethoxy-1,1ʹ-biphenanthrene (7) and bleformin …

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Identification, Antifungal And Plant Growth Promoting Activities Of Endophytic Actinomycetes From Orchids, Nisachon Tedsree Jan 2021

Identification, Antifungal And Plant Growth Promoting Activities Of Endophytic Actinomycetes From Orchids, Nisachon Tedsree

Chulalongkorn University Theses and Dissertations (Chula ETD)

Sixty-two endophytic actinomycetes were isolated from 15 Thai orchids. They were identified as Streptomyces (34 isolates), Micromonospora (21 isolate), Streptosporangium (2 isolates), Actinomadura (1 isolates), Amycolatopsis (3 isolates) and Pseudonocardia (1 isolates) based on their phenotypic, chemotaxonomic and genotypic characteristics. The antimicrobial activity screening revealed that Streptomyces (18 isolates), Micromonospora (12 isolates) and Streptosporangium (1 isolate) exhibited antimicrobial activity against Bacillus subtilis ATCC 6633, Kocuria rhizophila ATCC 9341, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and Candida albicans ATCC 10231. Strain DR2-2 could inhibit all tested pathogens. Based on 16S rRNA gene sequencing, strain DR6-1 …

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Extraction Of Cannabinoids Using Vegetable Oils And Its Uva-Photoprotective Effect On Human Skin Keratinocytes, Nur Alfan Muhammad Zen Jan 2021

Extraction Of Cannabinoids Using Vegetable Oils And Its Uva-Photoprotective Effect On Human Skin Keratinocytes, Nur Alfan Muhammad Zen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Nowadays, vegetable oil has attracted attention in its role as an alternative solvent. Its ability to dissolve various active compounds from plants has been proven by several previous studies. Moreover, other benefits of the use of vegetable oil as a solvent are affordable, non-toxic, and eco-friendly. The extraction process is one of the crucial factors that affect the quality of cannabis. Ethanol is still consistently the best solvent for cannabis extraction in terms of yield production compared to other organic solvents used in this study including oil whose yield cannot be calculated since vegetable oil cannot be evaporated. HPTLC fingerprint …

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Production And Characterization Of Escherichia Coli Outer Membrane Vesicles Displaying Anti-Muc1 Single-Chain Variable Fragment Via Spytag/Spycatcher System, Sedthawut Laotee Jan 2021

Production And Characterization Of Escherichia Coli Outer Membrane Vesicles Displaying Anti-Muc1 Single-Chain Variable Fragment Via Spytag/Spycatcher System, Sedthawut Laotee

Chulalongkorn University Theses and Dissertations (Chula ETD)

Outer membrane vesicles (OMVs) secreted from Gram-negative bacteria have been discovered for a decade. Special features like high stability, ease of production, and intrinsic adjuvanticity highlight the use of OMVs in diverse applications such as vaccination, bioimaging, and multifunctional delivery systems. Synthetic biological approaches can functionalize OMVs to present heterologous proteins by expressing genetic fusion of target protein to outer membrane proteins. However, complex proteins cannot be incorporated into the OMVs due to lack of some post-translational modifications in bacteria. Herein, we demonstrated a convenient method for presentation of versatile proteins in OMVs with the implementation of the bio-ligation system …

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3,4-Dihydroxy-5,4’-Dimethoxybibenzyl Sensitizes Non-Small Cell Lung Cancer Cells To Cisplatin-Induced Apoptosis Via P53 Upregulation, Hardyanti Eka Putri Jan 2021

3,4-Dihydroxy-5,4’-Dimethoxybibenzyl Sensitizes Non-Small Cell Lung Cancer Cells To Cisplatin-Induced Apoptosis Via P53 Upregulation, Hardyanti Eka Putri

Chulalongkorn University Theses and Dissertations (Chula ETD)

Lung cancer is a leading fatal malignancy with the highest number of cancer deaths. Targeting a specific protein regulating cancer progression and metastasis has been attracting much attention for the development of cancer therapy. Thus, this study was aimed to investigate the effect of 3,4-dihydroxy-5,4′-dimethoxybibenzyl (DS-1) in targeting MDM2-attenuating p53 function and inhibition EMT in lung cancer cells. The efficacy of DS-1 or combined with cisplatin in lung cancer cells was determined by MTT, nuclear staining, and Annexin V/PI assays. The expression of apoptosis- and EMT-related proteins was determined by western blot analysis. Metastatic behaviors were evaluated by cell shape …

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Cross-Cultural Adaptation And Psychometric Properties Of The Thai Version Of The Neurophysiology Of Pain Questionnaire, Pichet Pasangkayo Jan 2021

Cross-Cultural Adaptation And Psychometric Properties Of The Thai Version Of The Neurophysiology Of Pain Questionnaire, Pichet Pasangkayo

Chulalongkorn University Theses and Dissertations (Chula ETD)

Background: Pain neurophysiology knowledge is hypothesized to influence pain beliefs and physical performance in individuals with chronic low back pain (LBP). Valid and reliable measures of such knowledge is important to evaluate the pain treatment on this domain as well as to understand its role in both physical and psychological functions of individuals with chronic pain. This study aimed to culturally adapt the revised Neurophysiology of Pain Questionnaire into Thai (T-rNPQ), using the Functional Assessment of Chronic Illness Therapy (FACIT) methodology, and to evaluate its reliability and validity. Two hundred sixty-three individuals with chronic LBP completed the T-rNPQ and seven …

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Ab Initio Calculations On Superconducting Metal-Hydride And Perovskites For Energy Applications, Wiwittawin Sukmas Jan 2021

Ab Initio Calculations On Superconducting Metal-Hydride And Perovskites For Energy Applications, Wiwittawin Sukmas

Chulalongkorn University Theses and Dissertations (Chula ETD)

Superconducting hydrides and photovoltaic perovskites for energy applications are studied by means of the density functional theory-based calculations. First of all, the new decoration of Mg0.5Ca0.5H6 is confirmed thermodynamically stable over the pressures ranging from 200 to 400 GPa. The electronic properties reveal the possibility for this very material of becoming a superconductor under pressures. As a result, by performing electron-phonon coupling (EPC) calculations, Mg0.5Ca0.5H6 is found to superconduct at a maximum critical temperature of 288 K at 200 GPa, which completely exceeds those of its parent compounds, i.e. MgH6 and CaH6. Secondly, the van de Waals (vdW) interaction is …

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Effect Of Pongol Methyl Ether On Cancer Stem Cell Phenotypes In Human Non-Small Cell Lung Cancer Cells, Arnon Silapech Jan 2021

Effect Of Pongol Methyl Ether On Cancer Stem Cell Phenotypes In Human Non-Small Cell Lung Cancer Cells, Arnon Silapech

Chulalongkorn University Theses and Dissertations (Chula ETD)

Cancer stem cells (CSCs) are an important therapeutic target. The therapeutic agents targeting CSCs should lead to improved clinical outcomes. This study demonstrated the CSC suppressing activity of pongol methyl ether (PME), a pure compound from Millettia erythrocalyx Gagnep. CSC-suppressing effects were evaluated by colony formation assay, anchorage independent growth assay, spheroid formation assay and detection of CSC markers. The related CSC cell signals were evaluated by Western blot, immunofluorescence and molecular docking analysis. Proteins affected by PME treatment were subjected to bioinformatic analysis. Protein–protein interaction (PPI) networks were constructed by the Search Tool for Interactions of Chemicals (STITCH). The …

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Effects Of Low Degree Of High Ambient Temperature On Neural Pathways From Median Preoptic Nucleus To Arcuate Nucleus In Related To Food Intake., Pornsiri Suwannapaporn Jan 2021

Effects Of Low Degree Of High Ambient Temperature On Neural Pathways From Median Preoptic Nucleus To Arcuate Nucleus In Related To Food Intake., Pornsiri Suwannapaporn

Chulalongkorn University Theses and Dissertations (Chula ETD)

High ambient temperature (HTa) is one important environmental factor that decreases food intake (FI). It is well accepted that high degree HTa induced heat stress decreased FI by the activation of corticotropin-releasing factor (CRF) via hypothalamic pituitary adrenal axis. However, short-term low-degree HTa exposure has been demonstrated to decrease FI earlier than activated physiological and stress responses. This effect was related to the neuronal connection between the median preoptic nucleus (MnPO) and arcuate nucleus (Arc). Therefore, the present study aimed to investigate the effect of short-term low-degree HTa exposure on the activity of hypothalamic Arc in regarding to FI, and …

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The Efficacy Of Universal Ceramic Polishing Kit On The Surface Roughness Of Various Ceramic Types, Ekaluck Poosanthanasarn Jan 2021

The Efficacy Of Universal Ceramic Polishing Kit On The Surface Roughness Of Various Ceramic Types, Ekaluck Poosanthanasarn

Chulalongkorn University Theses and Dissertations (Chula ETD)

The objective of this vitro study was to determine the effect of polishing performance of a universal ceramic polishing kit on the surface roughness of various ceramics. The ceramic specimen size 5 x 7 x 4 mm of lithium disilicate glass ceramic (IPS e.max CAD, Ivoclar Vivadent, Liechtenstein), translucent zirconia (VITA YZ XT, VITA Zahnfabrik, Germany) and zirconia-reinforced lithium silicate (Celtra Duo, Dentsply Sirona, United States) (n=8) were fixed with clear resin in PVC block, 14 mm in diameter. The specimens were ground with fine diamond bur for 15 seconds to simulate clinical gross contouring.The two-step polishing process started with …

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Antifungal, Physical, And Mechanical Properties Of Tissue Conditioner Incorporated With Zinc Oxide Nanoparticles, Woraporn Homsiang Jan 2021

Antifungal, Physical, And Mechanical Properties Of Tissue Conditioner Incorporated With Zinc Oxide Nanoparticles, Woraporn Homsiang

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study evaluated the antifungal, physical, and mechanical properties of tissue conditioner incorporated with different amounts of zinc oxide nanoparticles (ZnOnps) at different storage times (0, 7, and 14 days). Specimens of 0, 5, 10, 15 wt% ZnOnps, or 15 wt% nystatin incorporated into tissue conditioner were fabricated. The direct contact test (n=6) was performed to evaluate the antifungal effect against C. albicans suspension. Scanning electron microscopy and energy dispersive X-ray spectroscopy (n=6) were used to observe the amount and distribution of superficial zinc element. The penetration depth (n=6) and tensile bond strength (n=8) were evaluated following ISO 13139. The …

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Facebook And Depression In Covid-19 Pandemic: The Role Of Time Spent On Information Overload And Depressive Symptoms With Trait Rumination As A Moderator, Vidchuda Taechajinda Jan 2021

Facebook And Depression In Covid-19 Pandemic: The Role Of Time Spent On Information Overload And Depressive Symptoms With Trait Rumination As A Moderator, Vidchuda Taechajinda

Chulalongkorn University Theses and Dissertations (Chula ETD)

The current COVID-19 pandemic has led to concerns for health and well-being, especially when the quarantine strategy such as social isolation has been implemented. Since social media has become an essential source of information, it might overwhelm its users by providing too much information which could yield many negative psychological outcomes, including depressive symptoms. The proposed research is designed to investigate the role of time spent on Facebook for searching and receiving information about COVID-19, as well as trait rumination on information overload and depressive symptoms. Non-clinical 140 active Facebook users aged between 18 to 34 who live in Bangkok …

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Effectiveness Of Positive Deviance, A Behavior Change Approach, To Improve Knowledge, Attitude And Practices On Dengue Prevention And Control In Selected Slums In Islamabad, Pakistan : A Mixed-Method Study, Muhammad Shafique Jan 2021

Effectiveness Of Positive Deviance, A Behavior Change Approach, To Improve Knowledge, Attitude And Practices On Dengue Prevention And Control In Selected Slums In Islamabad, Pakistan : A Mixed-Method Study, Muhammad Shafique

Chulalongkorn University Theses and Dissertations (Chula ETD)

This is the first practical application of positive deviance on dengue in the two selected low-income slums of Islamabad which was conducted during June-October 2020. A total of 112 participants (56 for intervention and 56 for the control group) participated in the study. The intervention arm received the positive deviance intervention through the identified positive deviance role models during the regular PD sessions, role plays, illustration competitions, and songs for two months. Three surveys were conducted, before the intervention, after two months, and after four months to assess the changes in knowledge, attitude, and practices. At the baseline, no statistically …

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Effect Of Line Application On Action And Coping Plans On Exercise Adherence And Functional Performance For Older Adults With Knee Pain In Suburban Area Of Bangkok Metropolis, Thailand : A Quasi Experimental Study, Pattaraporn Piwong Jan 2021

Effect Of Line Application On Action And Coping Plans On Exercise Adherence And Functional Performance For Older Adults With Knee Pain In Suburban Area Of Bangkok Metropolis, Thailand : A Quasi Experimental Study, Pattaraporn Piwong

Chulalongkorn University Theses and Dissertations (Chula ETD)

The known advantage of exercise for older adults who had knee pain is limited by low adherence to an exercise program. LINE application on action and coping plans do improve exercise adherence, knee functional performance and reduce knee pain. This quasi-experimental study determined the effect of LINE application on action and coping plans on exercise adherence, self-efficacy for exercise (SEE) , specific self-efficacy (task, maintenance, recovery), functional performance including knee range of motion (ROM), time up and go (TUG), 30-second chair stand (30CST) , knee outcome for activities of daily living (KOS-ADLS) and knee pain scale among older adults with …

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Association Between Physical Activity And Mental Health Among University Students In Bangladesh : A Cross Sectional Study, Sanjida Sultana Jan 2021

Association Between Physical Activity And Mental Health Among University Students In Bangladesh : A Cross Sectional Study, Sanjida Sultana

Chulalongkorn University Theses and Dissertations (Chula ETD)

Introduction: Mental health is considered one of the most significant topics of public health as it makes up an integral part of a person’s ability to lead a fulfilling life. University students are one of the most vulnerable groups to be affected by mental health disorders due to a change in their life and societal pressure. Lifestyle modifications like physical activity can be one way to combat these Mental Health problems. Although a lot of research has been carried out to analyze the health impact of Physical exercise, impact of it on Mental Health still lags, especially during the Covid-19 …

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Assessment Of Knowledge, Attitude And Usage On Personal Protective Equipment Among Mechanical Maintenance Workers Of A Power Generation Unit In Thailand, Titiphorn Tankian Jan 2021

Assessment Of Knowledge, Attitude And Usage On Personal Protective Equipment Among Mechanical Maintenance Workers Of A Power Generation Unit In Thailand, Titiphorn Tankian

Chulalongkorn University Theses and Dissertations (Chula ETD)

A Power Generation Unit has duty of electric power supply to the people by producing and distributing electric power. The mechanical maintenance department is one part of their responsibility to achieve the target. However, 207 accident cases were reported during 2012 to 2019. The main causes were found to be unsafe act and unsafe condition including improper usage and lack of personal protective equipment (PPEs). Thus, knowledge, attitude regarding to PPEs, its usage, and factors related to PPEs usage of mechanical maintenance workers need to be assessed. This survey study collected data through self-response questionnaire from 379 mechanical maintenance workers …

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Factors Associated To Mental Health And Stress Among Frontline Workers At Suvarnabhumi Airport During Covid-19 Pandemic, Thailand : A Cross-Sectional Study, Toonlaya Direkwutthikun Jan 2021

Factors Associated To Mental Health And Stress Among Frontline Workers At Suvarnabhumi Airport During Covid-19 Pandemic, Thailand : A Cross-Sectional Study, Toonlaya Direkwutthikun

Chulalongkorn University Theses and Dissertations (Chula ETD)

During COVID-19 pandemic,the frontline airport workers are the first group of people who have to screening all passengers from other countries to control and mitigate the imported case at the point of entry. The effects of COVID-19 are deeply down to individual health, especially the frontline airport workers who perform screening duties at the airport. The anxiousness and stress can occur from scarcity of equipment and afraid to be infected and can affects mental health and stress. This study is aimed to investigate the association between socio-demographic, job characteristics, and personal preventive measure factors to the mental health and stress …

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Prevalence And Associated Factors Of Food Insecurity During Covid-19 Pandemic In Bangkok, Thailand : A Cross-Sectional Study, Wimonmanee Mekkhum Jan 2021

Prevalence And Associated Factors Of Food Insecurity During Covid-19 Pandemic In Bangkok, Thailand : A Cross-Sectional Study, Wimonmanee Mekkhum

Chulalongkorn University Theses and Dissertations (Chula ETD)

The prevalence of food insecurity (FI) in Thailand has fluctuated throughout time. The COVID-19 pandemic had a substantial impact on Thailand's food system and the factors that contribute to food insecurity differ greatly across developed and developing countries. Consequently, this study may contribute as a step in achieving the SDGs by 2030. The aim was to estimate the prevalence and associated factors of food insecurity during COVID-19 pandemic among Bangkokian. A cross-sectional study was conducted over 440 Bangkokians and data were collected through self-administrated. The questionnaire included the general characteristics, associated factors and the Food Insecurity Experience Scale (FIES) in …

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Effects Of Selected Aroma Compounds On Physiological Activities And Emotions, Akarat Sivaphongthongchai Jan 2021

Effects Of Selected Aroma Compounds On Physiological Activities And Emotions, Akarat Sivaphongthongchai

Chulalongkorn University Theses and Dissertations (Chula ETD)

Current literature has revealed the effects and therapeutic properties of a wide variety of aromatic compounds but studies on the inhalation effects of single aromatic compounds in terms of central nervous system, emotional states and autonomic nervous system are still very limited. So, this study aimed to examine the physiological and emotional effects of selected volatile compounds inhalation namely d-borneol (BO), d-camphor (CH), methyl eugenol (ME), methyl chavicol (MC) using scientific techniques on autonomic nervous system (ANS), self-evaluated questionnaire on emotional states and brain wave activities. Sweet almond oil was used as a carrier oil and a diluent control. This …

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Compilation, Explanation And Restoration Of Moh Phon's Herbal Formulations For Skin Conditions, Qiaoling Ye Jan 2021

Compilation, Explanation And Restoration Of Moh Phon's Herbal Formulations For Skin Conditions, Qiaoling Ye

Chulalongkorn University Theses and Dissertations (Chula ETD)

Moh Phon’s traditional Thai medicine formularies were developed, compiled and recorded by Abhakara Kiartivongse or Moh Phon until 1915. This study aimed to elucidate traditional Thai medical wisdom regarding to Moh Phon formularies, especially for skin diseases. Herbal crude drugs in Moh Phon’s traditional Thai medicine formularies for skin diseases were compiled and authenticated for their vernacular and scientific names. The descriptions of plant species, ethnomedical uses, pharmacological activities and active constituents and toxicities were scientifically reviewed and established. The prevalence of plant species used in the formularies was analyzed. The formularies for 26 symptoms or diseases in the skin …

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