Digital Commons Network^™

Deep Learning For Automated Analysis Of Cellular And Extracellular Components Of The Foreign Body Response In Multiphoton Microscopy Images, Mattia Sarti, Maria Parlani, Luis Diaz-Gomez, Antonios G Mikos, Pietro Cerveri, Stefano Casarin, Eleonora Dondossola

Faculty, Staff and Student Publications

The Foreign body response (FBR) is a major unresolved challenge that compromises medical implant integration and function by inflammation and fibrotic encapsulation. Mice implanted with polymeric scaffolds coupled to intravital non-linear multiphoton microscopy acquisition enable multiparametric, longitudinal investigation of the FBR evolution and interference strategies. However, follow-up analyses based on visual localization and manual segmentation are extremely time-consuming, subject to human error, and do not allow for automated parameter extraction. We developed an integrated computational pipeline based on an innovative and versatile variant of the U-Net neural network to segment and quantify cellular and extracellular structures of interest, which is …

Recent Advances Of Integrative Bio-Omics Technologies To Improve Type 1 Diabetes (T1d) Care, Nisha Karwal, Megan Rodrigues, David D. Williams, Ryan Mcdonough, Diana Ferro

Manuscripts, Articles, Book Chapters and Other Papers

Type 1 diabetes (T1D) is a complex autoimmune disease that currently cannot be cured, only managed. Optimal treatment the of T1D symptoms, requires a multidisciplinary care team, including endocrinologists, educators, primary care providers, health care specialists, genetic counselors, and data scientists. This review summarizes how an integrative approach to T1D drives innovation and quality improvements in health care. Specifically, we highlight how “-omics” technologies facilitate the understanding of different aspects of the disease, including prevention, pathogenesis, diagnostics, and treatment. Furthermore, we explore how biological data can be combined with personal and electronic health records to tailor medical interventions to the …

Prevalence And Factors Associated With Mental Health Symptoms In Adults Undergoing Covid-19 Testing, Ikponmwosa Osaghae, Linh K Nguyen, Tong Han Chung, Olivia Moffitt, Yen-Chi L Le, Mark B Suh, Pooja N Prasad, Eric J Thomas, Christine D Gordon, Kevin O Hwang

Faculty, Staff and Student Publications

BACKGROUND AND OBJECTIVE: Understanding the mental health impact of the COVID-19 pandemic on persons receiving COVID-19 testing will help guide mental health interventions. We aimed to determine the association between sociodemographic factors and mental health symptoms at 8 weeks (baseline) after a COVID-19 test, and compare prevalence of mental health symptoms at baseline to those at 16-week follow-up.

MATERIALS AND METHODS: Prospective cohort study of adults who received outpatient COVID-19 testing at primary care clinics. Logistic regression analyses were used to assess the association between sociodemographic characteristics and COVID-19 test results with mental health symptoms. Mental health symptoms reported at …

Prevalence And Factors Associated With Mental Health Symptoms In Adults Undergoing Covid-19 Testing, Ikponmwosa Osaghae, Linh K Nguyen, Tong Han Chung, Olivia Moffitt, Yen-Chi L Le, Mark B Suh, Pooja N Prasad, Eric J Thomas, Christine D Gordon, Kevin O Hwang

Faculty, Staff and Student Publications

BACKGROUND AND OBJECTIVE: Understanding the mental health impact of the COVID-19 pandemic on persons receiving COVID-19 testing will help guide mental health interventions. We aimed to determine the association between sociodemographic factors and mental health symptoms at 8 weeks (baseline) after a COVID-19 test, and compare prevalence of mental health symptoms at baseline to those at 16-week follow-up.

MATERIALS AND METHODS: Prospective cohort study of adults who received outpatient COVID-19 testing at primary care clinics. Logistic regression analyses were used to assess the association between sociodemographic characteristics and COVID-19 test results with mental health symptoms. Mental health symptoms reported at …

Influenza Vaccination For The Prevention Of Cardiovascular Disease In The Americas: Consensus Document Of The Inter-American Society Of Cardiology And The Word Heart Federation, Álvaro Sosa Liprandi, María Inés Sosa Liprandi, Ezequiel José Zaidel, Gabriel M Aisenberg, Adrián Baranchuk, Eduardo Costa Duarte Barbosa, Gabriela Borrayo Sánchez, Bryce Alexander, Fernando Tomás Lanas Zanetti, Ricardo López Santi, Ana Girleza Múnera-Echeverri, Pablo Perel, Daniel Piskorz, Carlos Enrique Ruiz-Mori, Jorge Saucedo, Osiris Valdez, José Ramón González Juanatey, Daniel José Piñeiro, Fausto J Pinto, Fernando Stuardo Wyss Quintana

Faculty, Staff and Student Publications

BACKGROUND: Cardiovascular mortality is decreasing but remains the leading cause of death world-wide. Respiratory infections such as influenza significantly contribute to morbidity and mortality in patients with cardiovascular disease. Despite of proven benefits, influenza vaccination is not fully implemented, especially in Latin America.

OBJECTIVE: The aim was to develop a regional consensus with recommendations regarding influenza vaccination and cardiovascular disease.

METHODS: A multidisciplinary team composed by experts in the management and prevention of cardiovascular disease from the Americas, convened by the Inter-American Society of Cardiology (IASC) and the World Heart Federation (WHF), participated in the process and the formulation of …

Mir-9-1 Suppresses Cell Proliferation And Promotes Apoptosis By Targeting Uhrf1 In Lung Cancer, Cheng-You Jia, Wei Xiang, Ji-Bin Liu, Geng-Xi Jiang, Feng Sun, Jian-Jun Wu, Xiao-Li Yang, Rui Xin, Yi Shi, Dan-Dan Zhang, Wen Li, Zavuga Zuberi, Jie Zhang, Gai-Xia Lu, Hui-Min Wang, Pei-Yao Wang, Fei Yu, Zhong-Wei Lv, Yu-Shui Ma, Da Fu

Faculty, Staff and Student Publications

Lung cancer is listed as the most common reason for cancer-related death all over the world despite diagnostic improvements and the development of chemotherapy and targeted therapies. MicroRNAs control both physiological and pathological processes including development and cancer. A microRNA-9 to 1 (miR-9 to 1) overexpression model in lung cancer cell lines was established and miR-9 to 1 was found to significantly suppress the proliferation rate in lung cancer cell lines, colony formation in vitro, and tumorigenicity in nude mice of A549 cells. Ubiquitin-like containing PHD and RING finger domains 1 (UHRF1) was then identified to direct target of miR-9 …

Assessing How Understory Plant And Pollinator Interactions Are Affected By Differing Canopy Phenologies, Jack Everatt

School of Biological and Marine Sciences Theses

Advancements in springtime phenologies of plants and their pollinators have been widely observed within the northern hemisphere due to climate change. The observed advancements have led to concern over potential mismatching occurring between plant-pollinator interactions. The effect of forest canopies on understory phenological interactions has scarcely been investigated. We studied the effect of differing canopy closure parameters on understory phenological interactions within Plymbridge woods, Devon, UK, over a 6-month study period. The temporal overlap between canopy, understory flower and pollinators were investigated using Pianka overlap indices. Canopy phenology was modelled using NLSTIMEDIST package of R, giving three canopy parameters, canopy …

The Influence Of Emotion On Preservice Teachers As They Learn To Assess Student Learning, Frances Edwards

Australian Journal of Teacher Education

_{This paper explores the experience of emotion for eight preservice teachers as they learn to assess their students while concurrently being assessed. This qualitative study utilised semi-structured interviews and assessment-related artefacts. Findings indicate that emotional engagement influenced preservice teachers’ assessment decision making. The teachers also experienced emotional reactions as in turn they were assessed. This paper argues for the need of preservice teachers to be cognisant of the influence of emotion on themselves and their work, to allow them to better rationalise their assessment decision making and reflect on their practice.}

Teachers’ Experiences Of Educating Eal Students In Mainstream Primary And Secondary Classrooms, Jessica Premier

Australian Journal of Teacher Education

Many schools in Victoria, Australia, are multicultural, with students coming from a variety of cultures and backgrounds. Content area teachers often educate EAL students in their classrooms, even though they may not have specialised EAL teaching qualifications. This paper presents the experiences of primary and secondary teachers working in multicultural schools in Victoria. It explores the way in which teachers meet the needs of EAL students in their classrooms, and the support that is available to assist them to do so. This paper reports that teaching practice, school leadership, professional learning, and identity, influence the way in which teachers educate …

Shifting Emergency Remote Teaching Into The Future Of Kentucky Education, Amy Argo

Murray State Theses and Dissertations

During the 2020 COVID-19 pandemic, Kentucky educators discovered the difficulties of a rapid transition to emergency remote teaching. This phenomenological, qualitative research study explores middle and high school teachers’ lived experiences of teaching Kentucky public school students during the shutdown of school buildings during the spring and fall 2020 semesters.

Twenty-nine educator participants freely discussed their greatest successes and barriers concerning parental support, teacher mental health, communication, digital divides, student motivation, and teacher preparedness. This project gives voice to the teachers of Kentucky with a practical significance: effective online instruction can only occur if educators are properly prepared to teach …

Opportunities, Barriers, And Recommendations In Down Syndrome Research., James A. Hendrix, Angelika Amon, Leonard Abbeduto, Stamatis Agiovlasitis, Tarek Alsaied, Heather A. Anderson, Lisa J. Bain, Nicole Baumer, Anita Bhattacharyya, Dusan Bogunovic, Kelly N. Botteron, George Capone, Priya Chandan, Isabelle Chase, Brian Chicoine, Cécile Cieuta-Walti, Lara R. Deruisseau, Sophie Durand, Anna Esbensen, Juan Fortea, Sandra Giménez, Ann-Charlotte Granholm, Laura J. Hahn, Elizabeth Head, Hampus Hillerstrom, Lisa M. Jacola, Matthew P. Janicki, Joan M. Jasien, Angela R. Kamer, Raymond D. Kent, Bernard Khor, Jeanne B. Lawrence, Catherine Lemonnier, Amy Feldman Lewanda, William Mobley, Paul E. Moore, Linda Pollak Nelson, Nicolas M. Oreskovic, Ricardo S. Osorio, David Patterson, Sonja A. Rasmussen, Roger H. Reeves, Nancy Roizen, Stephanie Santoro, Stephanie L. Sherman, Nasreen Talib, Ignacio E. Tapia, Kyle M. Walsh, Steven F. Warren, A Nicole White, Guang William Wong, John S. Yi

Manuscripts, Articles, Book Chapters and Other Papers

BACKGROUND: Recent advances in medical care have increased life expectancy and improved the quality of life for people with Down syndrome (DS). These advances are the result of both pre-clinical and clinical research but much about DS is still poorly understood. In 2020, the NIH announced their plan to update their DS research plan and requested input from the scientific and advocacy community.

OBJECTIVE: The National Down Syndrome Society (NDSS) and the LuMind IDSC Foundation worked together with scientific and medical experts to develop recommendations for the NIH research plan.

METHODS: NDSS and LuMind IDSC assembled over 50 experts across …

Hacking The Seams: Elevating Digital Autonomy And Agency For Humans, Richard S. Whitt

Colorado Technology Law Journal

No abstract provided.

Effect Of Foliar Application Of Nitrogen-Phosphorus-Potassium Fertilizers On Nutrient Uptake And Protein Content Of Maize, Drocelle Nirere, S. R Mbaraka, F Murorunkwere, F. R Musana, H Rukangantambara

All Rsif Scholars' Publications

The effect of foliar application of nitrogen-phosphorus-potassium (NPK) fertilizers on nutritional status and yield of maize (Zea mays L.) were investigated through a study conducted in a zonal agricultural research station of University of Agricultural Sciences, GKVK Bengaluru (India). The experiment was laid out in randomized complete block design. The treatments consisted of two levels of soil application (75 and 100%) of recommended dose of fertilizer (RDF) and foliar applications of NPK (19:19:19). The fertilizers were applied at 6-leaf, tasselling, knee height and tasseling growth stages. Nutrient uptake, protein content and available soil nutrients at harvest were analyzed. Results indicated …

Genomic Characterization Of Fluoroquinolone-Resistant Thermophilic Campylobacter Strains Isolated From Layer Chicken Feces In Gangneung, South Korea By Whole-Genome Sequencing, Gahamanyi Noel, Dae-Geun Song, Kye-Yoon Yoon, Leonard E. G. Mboera, Mecky I. Matee, Dieudonné Mutangana, Erick V. G Komba, Cheol-Ho Pan, Raghavendra G. Amachawadi

All Rsif Scholars' Publications

Thermophilic Campylobacter species of poultry origin have been associated with up to 80% of human campylobacteriosis cases. Layer chickens have received less attention as possible reservoirs of Campylobacter species. Initially, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of two archived Campylobacter isolates (Campylobacter jejuni strain 200605 and Campylobacter coli strain 200606) from layer chickens to five antimicrobials (ciprofloxacin, nalidixic acid, erythromycin, tetracycline, and gentamicin) were determined using broth microdilution while the presence of selected antimicrobial resistance genes was performed by polymerase chain reaction (PCR) using specific primers. Whole-genome sequencing (WGS) was performed by the Illumina HiSeq X …

Complete Genome Analysis Of African Swine Fever Virus Responsible For Outbreaks In Domestic Pigs In 2018 In Burundi And 2019 In Malawi, Jean Nepomuscene Hakizimana, Jean B. Ntirandekura, Clara Yona, Lionel Nyabongo, Gladson Kamwendo, Julius L.C Chulu, Désiré Ntakirutimana, Olivier Kamana, Hans Nauwynck, Gerald Misinzo

All Rsif Scholars' Publications

Several African swine fever (ASF) outbreaks in domestic pigs have been reported in Burundi and Malawi and whole-genome sequences of circulating outbreak viruses in these countries are limited. In the present study, complete genome sequences of ASF viruses (ASFV) that caused the 2018 outbreak in Burundi (BUR/18/Rutana) and the 2019 outbreak in Malawi (MAL/19/Karonga) were produced using Illumina next-generation sequencing (NGS) platform and compared with other previously described ASFV complete genomes. The complete nucleotide sequences of BUR/18/Rutana and MAL/19/Karonga were 176,564 and 183,325 base pairs long with GC content of 38.62 and 38.48%, respectively. The MAL/19/Karonga virus had a total …

Molecular Detection Of Campylobacter Species From Human And Cattle Faecal Samples In Kilosa District, Tanzania, Gahamanyi Noel, Leonard E.G Mboera, Mecky I. Matee, Dieudonné Mutangana, Raghavendra G. Amachawadi, Kye-Yoon Yoon, Mr. Humphrey, Cheol-Ho Pan, Erick V.G Komba

All Rsif Scholars' Publications

A growing number of Campylobacter species other than C. jejuni and C. coli have been considered as emerging human and animal pathogens but their contribution to human gastroenteritis is poorly documented. This study aimed at detecting Campylobacter species from human and cattle faecal samples in Kilosa District, Tanzania using molecular techniques without culture. Seventy (70) faecal samples were collected from five diarrheic and 65 non-diarrheic human patients attending Kilosa District Hospital in Tanzania from July to October 2019. During the same period, 30 faecal samples were also collected from healthy cattle in the same district. Genus and species identification of …

Antimicrobial Resistance Profiles, Virulence Genes, And Genetic Diversity Of Thermophilic Campylobacter Species Isolated From A Layer Poultry Farm In Korea, Gahamanyi Noel, Dae-Geun Song, Kye-Yoon Yoon, Leonard E. G Mboera, Mecky I. Matee, Mutangana Dieudonne Dieudonne, Raghavendra G. Amachawadi, Erick Komba, Cheol-Ho Pan

All Rsif Scholars' Publications

Thermophilic Campylobacter species are among the major etiologies of bacterial enteritis globally. This study aimed at assessing the antimicrobial resistance (AMR) profiles, virulence genes, and genetic diversity of thermophilic Campylobacter species isolated from a layer poultry farm in South Korea. One hundred fifty-three chicken feces were collected from two layer poultry farms in Gangneung, South Korea. The Campylobacter species were isolated by cultural techniques, while PCR and sequencing were used for species confirmation. Antimicrobial susceptibility testing for six antimicrobials [ciprofloxacin (CIP), nalidixic acid (NAL), sitafloxacin (SIT), erythromycin (ERY), tetracycline (TET), and gentamicin (GEN)] was carried out by broth microdilution. Three …

An Assessment Of The Epidemiology And Socioeconomic Impact Of The 2019 African Swine Fever Outbreak In Ngara District, Western Tanzania, Jean Nepomuscene Hakizimana, C.C Kivumbi, C Yona, Gerald M. Misinzo

All Rsif Scholars' Publications

African swine fever (ASF) is a contagious viral transboundary animal disease affecting domestic pigs caused by ASF virus (ASFV). This study was conducted in order to determine the genetic characteristics, risk factors and socioeconomic impact of an ASF outbreak in 2019 in Ngara, western Tanzania. Tissue samples from dead domestic pigs with clinical picture suggestive of ASF infection were collected for ASF confirmation and genetic characterization of ASFV. Data on the risk factors and socioeconomic impact associated with the ASF outbreak were collected from consenting farmers using a semi-structured questionnaire. Disease confirmation was done by detection of genomic ASFV DNA …

Exposure To Salinity And Light Spectra Regulates Glucosinolates, Phenolics, And Antioxidant Capacity Of Brassica Carinata L. Microgreens, Sylvia Wairimu Maina, Da Hye Ryu, Jwa Yeong Cho, Da Seul Jung, Jai-Eok Park, Chu Won Nho, Gaymary Bakari, Gerald Misinzo, Je Hyeong Jung, Seung-Hoon Yang, Ho-Youn K Kim

All Rsif Scholars' Publications

The effect of salt treatment on Brassica carinata (BC) microgreens grown under different light wavelengths on glucosinolates (GLs) and phenolic compounds were evaluated. Quantifiable GLs were identified using ultra-high performance-quadrupole time of flight mass spectrometry. Extracts’ ability to activate antioxidant enzymes (superoxide dismutase (SOD) and catalase (CAT)) was evaluated on human colorectal carcinoma cells (HCT116). Furthermore, BC compounds’ ability to activate expression of nuclear transcription factor-erythroid 2 related factor (Nrf2) and hemeoxygenase- 1 (HO-1) proteins was examined using specific antibodies on HCT116 cells. Sinigrin (SIN) was the abundant GLs of the six compounds identified and its content together with total …

Comparing Effectiveness, Cost- And Time-Efficiency Of Control Options For Xanthomonas Wilt Of Banana Under Rwandan Agro-Ecological Conditions, Guy Blomme, Petronille Dusingizimana, Jules Ntamwira, Elizabeth Kearsley, Svetlana Gaidashova, Anne Rietveld, Boudy Van Schagen, Walter Ocimati

All Rsif Scholars' Publications

Xanthomonas wilt of banana (XW) is a major disease affecting banana throughout East and Central Africa (ECA). Initial control was through complete diseased mat uprooting (CDMU), which had limited adoption due to high labour demands and cost. Further research demonstrated single dis- eased stem removal (SDSR) was an effective, less labour intensive and less costly alternative to CDMU. A comparative assessment of the two con- trol practices was needed to foster uptake by policy makers, especially in countries like Rwanda where SDSR had not been tested or scaled up. To test SDSR in Rwanda, a study was conducted in farmers’ …

Nanobodies: Their Potential For Applications In Biotechnology, Diagnosis And Antiviral Properties In Africa; Focus On Application In Agriculture, Faith Njeri Njeru, Paul Mbogo Kusolwab

All Rsif Scholars' Publications

Antibodies are key elements in routine immunological tests, having a long history in detection of plant pathogens due to their specificity, reliability and fast performance. Heavy chain-only antibodies (HCAbs), circulating in the blood of camels are IgG isotypes that lack the light (L) chains and the heavy (H) chain is depleted of the first constant domain (CH1). The HCAbs interact with the antigen by virtue of only one single variable domain, referred to as VHH or nanobody (Nb). Owing to their unique properties: nanometer scale size, robust structure, stable and soluble behaviour in aqueous solution, high affinity and specificity for …

Elucidation Of Multi-Origin Inula Helenium L. For Antimicrobial Activity And Heavy Metal Profiling As An Exemplar For The Use And Regulation Of Plants As Antibiotics., Ciara Ruth Kenny

Theses

An impending post-antibiotic era looms, thus new sources of therapeutic chemical entities are crucial to sustain effective antimicrobial chemotherapy worldwide. Plant natural products are a source of accessible, structurally diverse compounds that provide therapeutic potential. The pharmacological applications of plants in medicine can be guided by the attestation of traditional use or the application of this knowledge to uncover new leads in the drug discovery process. The range of modified in vitro methods commonly used to investigate preclinical antimicrobial efficacy of plant-derived natural products, and the associated limitations and challenges in the provision of new antimicrobial drugs from plants is …

Evaluation Of A ‘Well-Elderly’ Physical Activity Programme, Robert Purcell

Theses

Both in Ireland and internationally people are living longer and there is an increasing proportion of older adults in the population (Clark 2020). As such, the maintenance of health and physical function for older adults is of critical importance. Physical activity is widely acknowledged as being crucial for the maintenance of positive health, particularly for older adults (Spartano et al., 2019). Research has suggested that physical activity programmes are an effective means of engaging older adults in physical activity for the promotion of positive health (Sherrington et al., 2017). The central aim of this research project was to explore the …

Effects Of Body Temperature On Initial Bite Force In Three Species Of Rock- And Crevice-Dwelling Lizards From Mexico, Guillermo A. Woolrich-Piña, Geoffrey R. Smith, Norberto Martínez-Méndez, Julio A. Lemos-Espinal, Héctor Gadsden-Esparz

Faculty Publications

No abstract provided.

Fecal Pellet Mass As A Function Of Body Size, Color Morph, And Season In Plethodon Cinereus (Green, 1818) (Amphibia: Plethodontidae), Geoffrey R. Smith, Abhishek V. Henry, Logan E. Smith, Wesley O. Smith

Faculty Publications

No abstract provided.

Data Science In Undergraduate Life Science Education: A Need For Instructor Skills Training, Nathan C. Emery, Erika Crispo, Sarah R. Supp, Kaitlin J. Farrell, Andrew J. Kerkhoff

Faculty Publications

No abstract provided.

A Statistical Analysis Of Drug Seizures And Opioid Overdose Deaths In Ohio From 2014 To 2018, David White, Lam Tran, Lin Ma

Faculty Publications

No abstract provided.

Bright Chromenylium Polymethine Dyes Enable Fast, Four-Color "In Vivo" Imaging With Shortwave Infrared Detection, Emily D. Cosco, Bernardo A. Arús, Anthony L. Spearman, Timothy Luke Atallah, Irene Lim

Faculty Publications

No abstract provided.

Data Science In Undergraduate Life Science Education: A Need For Instructor Skills Training, Nathan C. Emery, Erika Crispo, Sarah R. Supp, Kaitlin J. Farrell, Andrew J. Kerkhoff

Faculty Publications

No abstract provided.

Estimating The Movements Of Terrestrial Animal Populations Using Broad-Scale Occurrence Data, Sarah R. Supp, Gil Bohrer, John Fieberg, Frank A. La Sorte

Faculty Publications

No abstract provided.