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2021

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Progress And Prospect Of Anodic Oxidation For The Remediation Of Perfluoroalkyl And Polyfluoroalkyl Substances In Water And Wastewater Using Diamond Electrodes, Sean T. Mcbeath, Adrián Serrano Mora, Fatemeh Asadi Zeidabadi, Brooke K. Mayer, Patrick J. Mcnamara, Madjid Mohseni, Michael R. Hoffman, Nigel J. D. Graham Dec 2021

Progress And Prospect Of Anodic Oxidation For The Remediation Of Perfluoroalkyl And Polyfluoroalkyl Substances In Water And Wastewater Using Diamond Electrodes, Sean T. Mcbeath, Adrián Serrano Mora, Fatemeh Asadi Zeidabadi, Brooke K. Mayer, Patrick J. Mcnamara, Madjid Mohseni, Michael R. Hoffman, Nigel J. D. Graham

Civil and Environmental Engineering Faculty Research and Publications

Although diamond electrodes are widely used in the field of electroanalysis and sensing, their application in the field of environmental engineering has yet to be fully realized. Many research studies have considered their potential application in water and wastewater treatment, where the in-situ electrochemical process can avoid the need for chemical additives by facilitating the oxidation of pollutants on the electrode surface or mediated by electrochemically synthesized oxidants in solution. Diamond-based electro-oxidation can effectively treat a number of organic micropollutants and is now being evaluated for the abatement of perfluoroalkyl and polyfluoroalkyl substances, which pose health concerns and are ubiquitous …


New Common Fixed Points For Total Asymptotically Nonexpansive Mapping In Cat(0) Space, Ahmed Jameel Kadhim Dec 2021

New Common Fixed Points For Total Asymptotically Nonexpansive Mapping In Cat(0) Space, Ahmed Jameel Kadhim

Baghdad Science Journal

Strong and ∆-convergence for a two-step iteration process utilizing asymptotically nonexpansive and total asymptotically nonexpansive noneslf mappings in the CAT(0) spaces have been studied. As well, several strong convergence theorems under semi-compact and condition (M) have been proved. Our results improve and extend numerous familiar results from the existing literature.


Comparison Of Different Image Data Augmentation Approaches, Loris Nanni, Michelangelo Paci, Sheryl Brahnam, Alessandra Lumini Dec 2021

Comparison Of Different Image Data Augmentation Approaches, Loris Nanni, Michelangelo Paci, Sheryl Brahnam, Alessandra Lumini

College of Business

Convolutional neural networks (CNNs) have gained prominence in the research literature on image classification over the last decade. One shortcoming of CNNs, however, is their lack of generalizability and tendency to overfit when presented with small training sets. Augmentation directly confronts this problem by generating new data points providing additional information. In this paper, we investigate the performance of more than ten different sets of data augmentation methods, with two novel approaches proposed here: one based on the discrete wavelet transform and the other on the constant-Q Gabor transform. Pretrained ResNet50 networks are finetuned on each augmentation method. Combinations of …


Incorporation Of Zinc In Pre-Alloyed Cuin[Zn]S2/Zns Quantum Dots, Jean Carlos Morales Orocu Dec 2021

Incorporation Of Zinc In Pre-Alloyed Cuin[Zn]S2/Zns Quantum Dots, Jean Carlos Morales Orocu

Graduate Theses and Dissertations

Since the early 2000s heavy-metal-free quantum dots (QDs) such as CuInS2/ZnS have attempted to replace CdSe, their heavy-metal-containing counterparts. CuInS2/ZnS is synthesized in a two-step process that involves the fabrication of CuInS2 (CIS) nanocrystals (NCs) followed by the addition of zinc precursors. Instead of the usual core/shell architecture often exhibited by binary QDs, coating CIS QDs results in alloyed and/or partially alloyed cation-exchange (CATEX) QDs. The effect that zinc has on the properties of CIS NCs was studied by incorporating zinc during the first step of the synthesis. Different In:Cu:Zn ratios were employed in this study, maintaining a constant 4:1 …


Evaluation Of Two Neonatal Challenge Models For Broiler Chickens And Their Effects On Early Performance Parameters, Callie Selby Dec 2021

Evaluation Of Two Neonatal Challenge Models For Broiler Chickens And Their Effects On Early Performance Parameters, Callie Selby

Graduate Theses and Dissertations

Formaldehyde fumigation in poultry hatch cabinets has been utilized for sanitation of hatching eggs for over a century. Formaldehyde is utilized to minimize pathogenic microbes on the surface of the egg as well as the microbial bloom during the hatching process. While formaldehyde is effective, its use is regulated in the United States and Europe due to its carcinogenic nature. Formaldehyde has been shown to damage the cuticle of the egg, cause embryonic death, and damage the epithelial lining of the respiratory tract of freshly hatched chicks, predisposing them for respiratory infection. Alternatives for formaldehyde fumigation must be identified and …


Efficacy Of Selected Powdered Floor Treatments And Turmeric Rhizome Powder Against Salmonella, E. Coli, And L. Monocytogenes On Polyurethane-Concrete Flooring Material Carriers, Robert Sidney Fuller Dec 2021

Efficacy Of Selected Powdered Floor Treatments And Turmeric Rhizome Powder Against Salmonella, E. Coli, And L. Monocytogenes On Polyurethane-Concrete Flooring Material Carriers, Robert Sidney Fuller

Graduate Theses and Dissertations

The flooring in a food processing environment can become contaminated with pathogenic bacteria in many ways including foot and equipment traffic, incoming materials, and drain backups. Turmeric (Curcuma longa L.) rhizome powder and seventeen other commercially available powdered floor treatments with a variety of active ingredients including quaternary ammonium compounds (QAC), sodium percarbonate and similar perhydrates (SPC), urea, and borax, were investigated for reducing the levels of pathogens on flooring thereby reducing the risk of cross-contamination from the floor to food contact surfaces. Some of the commercially available floor treatments were Environmental Protection Agency (EPA) registered antimicrobials while others were …


Analysis Of Bacteriorhodopsin Suspended In A Bilayer Lipid Membrane, Moath Alhejji Dec 2021

Analysis Of Bacteriorhodopsin Suspended In A Bilayer Lipid Membrane, Moath Alhejji

Graduate Theses and Dissertations

The bacteriorhodopsin protein’s unique characteristic of proton pumping can convert light energy to electric energy. The aim of this research was to generate photocurrent using bacteriorhodopsin in a bi-layer lipid membrane. Lipid monolayer and bilayer were formed using painting and folding methods, respectively. Capacitance and resistance of the lipid membranes were measured and used to validate the best methodology. My results show that the folding method is more efficient in incorporating Bacteriorhodopsin. The photocurrent was generated by illuminating a green laser (532 nm) on the bilayer lipid membranes. The patch clamp electrophysiology technique was used to apply voltage across the …


Sedimentary Dna And Molecular Evidence For Early Human Occupation Of The Faroe Islands, Lorelei Curtin, William J. D’Andrea, Nicholas L. Balascio, Sabrina Shirazi, Beth Shapiro, Gregory A. De Wet, Raymond S. Bradley, Jostein Bakke Dec 2021

Sedimentary Dna And Molecular Evidence For Early Human Occupation Of The Faroe Islands, Lorelei Curtin, William J. D’Andrea, Nicholas L. Balascio, Sabrina Shirazi, Beth Shapiro, Gregory A. De Wet, Raymond S. Bradley, Jostein Bakke

Geosciences: Faculty Publications

The Faroe Islands, a North Atlantic archipelago between Norway and Iceland, were settled by Viking explorers in the mid-9th century CE. However, several indirect lines of evidence suggest earlier occupation of the Faroes by people from the British Isles. Here, we present sedimentary ancient DNA and molecular fecal biomarker evidence from a lake sediment core proximal to a prominent archaeological site in the Faroe Islands to establish the earliest date for the arrival of people in the watershed. Our results reveal an increase in fecal biomarker concentrations and the first appearance of sheep DNA at 500 CE (95% confidence interval …


Leadership Influence On Collective Teacher Efficacy In Multi-Cultural Teams: A Mixed-Methods Study, Yaru Zhou Dec 2021

Leadership Influence On Collective Teacher Efficacy In Multi-Cultural Teams: A Mixed-Methods Study, Yaru Zhou

Education Dissertations

As evidence for positive effects of collective teacher efficacy on student performance and teacher well-being grow, increasing efforts have been made worldwide to understand how teachers’ beliefs in the team’s capacity form in schools. This study adopts a mixed-methods approach; 90 teachers from seven international schools in China participated in the survey, and eight teachers from these schools joined focus group interviews. This paper seeks to understand the relationship between teachers’ perceptions of instructional school leadership and collective efficacy, and how individual leaders’ character strength of encouragement and structural factors, including school levels and cultural dimensions, affect the relationship. Quantitative …


Complete Vertebrate Mitogenomes Reveal Widespread Repeats And Gene Duplications, Giulio Formenti, Arang Rhie, Jennifer Balacco, Bettina Haase, Jacquelyn Mountcastle, Olivier Fedrigo, Samara Brown, Marco Rosario Capodiferro, Farooq O. Al-Ajli, Roberto Ambrosini, Peter Houde, Sergey Koren, Karen Oliver, Michelle Smith, Jason Skelton, Emma Betteridge, Jale Dolucan, Craig Corton, Iliana Bista, James Torrance, Alan Tracey, Jonathan Wood, Marcela Uliano-Silva, Kerstin Howe, Shane Mccarthy, Sylke Winkler, Woori Kwak, Jonas Korlach, Arkarachai Fungtammasan, Et Al, Tanya M. Lama Dec 2021

Complete Vertebrate Mitogenomes Reveal Widespread Repeats And Gene Duplications, Giulio Formenti, Arang Rhie, Jennifer Balacco, Bettina Haase, Jacquelyn Mountcastle, Olivier Fedrigo, Samara Brown, Marco Rosario Capodiferro, Farooq O. Al-Ajli, Roberto Ambrosini, Peter Houde, Sergey Koren, Karen Oliver, Michelle Smith, Jason Skelton, Emma Betteridge, Jale Dolucan, Craig Corton, Iliana Bista, James Torrance, Alan Tracey, Jonathan Wood, Marcela Uliano-Silva, Kerstin Howe, Shane Mccarthy, Sylke Winkler, Woori Kwak, Jonas Korlach, Arkarachai Fungtammasan, Et Al, Tanya M. Lama

Biological Sciences: Faculty Publications

Background: Modern sequencing technologies should make the assembly of the relatively small mitochondrial genomes an easy undertaking. However, few tools exist that address mitochondrial assembly directly. Results: As part of the Vertebrate Genomes Project (VGP) we develop mitoVGP, a fully automated pipeline for similarity-based identification of mitochondrial reads and de novo assembly of mitochondrial genomes that incorporates both long (> 10 kbp, PacBio or Nanopore) and short (100–300 bp, Illumina) reads. Our pipeline leads to successful complete mitogenome assemblies of 100 vertebrate species of the VGP. We observe that tissue type and library size selection have considerable impact on mitogenome …


An Outbreak Of Canine Schistosomiasis In Utah Acquisition Of A New Snail Host (Galba Humilis) By Heterobilharzia Americana, A Pathogenic Parasite On The Move, Eric S. Loker, Scott Z. Dolginow, Suzanne Pape, Colin D. Topper, Randall J. Dejong Dec 2021

An Outbreak Of Canine Schistosomiasis In Utah Acquisition Of A New Snail Host (Galba Humilis) By Heterobilharzia Americana, A Pathogenic Parasite On The Move, Eric S. Loker, Scott Z. Dolginow, Suzanne Pape, Colin D. Topper, Randall J. Dejong

University Faculty Publications and Creative Works

Parasites with complex life cycles engaging multiple host species living among different environments well-exemplify the value of a cross-cutting One Health approach to understanding fundamental concerns like disease emergence or spread. Here we provide new information regarding a pathogenic schistosome trematode parasite of both wild and domestic mammals that has recently expanded its known range from mesic/wet environments of the southeastern United States to the arid southwest. In 2018, 12 dogs living near a man-made pond in Moab, Utah, were found positive for Heterobilharzia americana, the most westerly report of this endemic North American schistosome, and the first from Utah. …


Comparison Of Sample Types From White-Tailed Deer (Odocoileus Virginianus) For Dna Extraction And Analyses, Jessie Edson, Justin Brown, William L. Miller, David Walter Dec 2021

Comparison Of Sample Types From White-Tailed Deer (Odocoileus Virginianus) For Dna Extraction And Analyses, Jessie Edson, Justin Brown, William L. Miller, David Walter

University Faculty Publications and Creative Works

Collection of biological samples for DNA is necessary in a variety of disciplines including disease epidemiology, landscape genetics, and forensics. Quantity and quality of DNA varies depending on the method of collection or media available for collection (e.g., blood, tissue, fecal). Blood is the most common sample collected in vials or on Whatman Flinders Technology Associates (FTA) cards with short- and long-term storage providing adequate DNA for study objectives. The focus of this study was to determine if biological samples stored on Whatman FTA Elute cards were a reasonable alternative to traditional DNA sample collection, storage, and extraction. Tissue, nasal …


An Investigation Of Phosphorus Removal By Purple Bacteria In Dairy Wastewater Lagoons, Lauren M. Gomez, Andrew J. Schuler, Jose M. Cerrto, David T. Hanson Dec 2021

An Investigation Of Phosphorus Removal By Purple Bacteria In Dairy Wastewater Lagoons, Lauren M. Gomez, Andrew J. Schuler, Jose M. Cerrto, David T. Hanson

Civil Engineering ETDs

The objective of this study was to determine whether the polyphosphate metabolism in purple photosynthetic bacteria (PPB) is similar to that of wastewater polyphosphate-accumulating organisms (PAOs) such as Accumulibacter. Dairy lagoon samples were studied in continuous and batch laboratory tests to evaluate whether cyclic light and dark conditions for PPB are similar to aerobic and anaerobic conditions for PAOs in domestic wastewater treatment systems. 16s rRNA gene amplicon sequencing indicated the presence of the purple sulfur bacterium Thiolamprovum (53.92%) in one lagoon source, and Thiodictyon (3.50%) in a second lagoon. Phosphorus contents were at least 0.7% higher than the …


Applying Behavioral And Physiological Measures To Assess The Relative Impact Of The Prolonged Covid-19 Pandemic Closure On Two Mammal Species At The Oregon Zoo: Cheetah (A. Jubatus) And Giraffe (G. C. Reticulata And G. C. Tippelskirchii), Laurel Fink, Candace D. Scarlata, Becca Vanbeek, Todd Bodner, Nadja C. Wielebnowski Dec 2021

Applying Behavioral And Physiological Measures To Assess The Relative Impact Of The Prolonged Covid-19 Pandemic Closure On Two Mammal Species At The Oregon Zoo: Cheetah (A. Jubatus) And Giraffe (G. C. Reticulata And G. C. Tippelskirchii), Laurel Fink, Candace D. Scarlata, Becca Vanbeek, Todd Bodner, Nadja C. Wielebnowski

Biology Faculty Publications and Presentations

The effect of visitor presence on zoo animals has been explored in numerous studies over the past two decades. However, the opportunities for observations without visitors have been very limited at most institutions. In 2020, the Oregon Zoo was closed, in response to the global SARSCoV-2 (COVID-19) pandemic, from 15 March 2020 to 12 July 2020, resulting in approximately four consecutive months without visitor presence. This study aimed to quantify potential behavioral and hormonal changes expressed during two transition periods in zoo visitor attendance: the initial time period before and after closure in March 2020 and time before and after …


Evaluation Of A Novel Approach For Assessing Biological Activity In Agricultural Soils, Karla Melgar Dec 2021

Evaluation Of A Novel Approach For Assessing Biological Activity In Agricultural Soils, Karla Melgar

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Soil health is a key factor impacting soil resilience and fertility in crop production systems. Favorable soil physical and biological conditions facilitate plant nutrient absorption and nutrient cycling. Demonstrating to farmers the impacts and changes in soil biological activity under different soil management practices has been a challenge due to the limited availability of inexpensive tools for quantifying this component of soil health. The primary goal of this study was to present a simple and readily accessible tool for evaluating soil biological activity to promote the use of organic amendments in crop fields. Research plots were established in two studies …


High-Frequency Unmanned Aircraft Flights For Crop Canopy Imaging During Diurnal Moisture Stress, Suresh Pradhyun Kashyap Dec 2021

High-Frequency Unmanned Aircraft Flights For Crop Canopy Imaging During Diurnal Moisture Stress, Suresh Pradhyun Kashyap

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Previous research has used unmanned aerial vehicle (UAV) technology for calculating CWSI (Crop Water Stress Index) values in the context of irrigation scheduling. Typically, these estimations were taken at one time of day, usually near shortly after solar noon. A significant limitation with these CWSI values is that the UAV thermal imagery captured at this point in time can be affected by various factors like atmospheric air temperature, solar radiation, wind speed, relative humidity, and other micrometeorological disturbances in the air. In order to address these temporal effects, high-frequency UAV flights were conducted over different daylight hours to analyze and …


Assessment Of Soil Sampling Equipment For Guiding Fertiliser Decisions, David Weaver, Robert Summers, David Rogers, Peta Richards, David Rowe Dec 2021

Assessment Of Soil Sampling Equipment For Guiding Fertiliser Decisions, David Weaver, Robert Summers, David Rogers, Peta Richards, David Rowe

Resource management technical reports

A range of methods, technologies and equipment are used to collect representative composite soil samples from paddocks. Once collected, soil samples are analysed for various parameters that provide evidence to guide fertiliser decisions. The sampling methods, technologies and equipment used must result in samples that consistently represent the parameter of interest.

Soil sampling technology and equipment has advanced from manual devices (such as pogo-stick-style foot-thrust core samplers [pogo]) to a variety of mechanised core-thrust samplers and augers that are fitted to vehicles or battery drills. Each device may function differently under different conditions, and each requires differing levels of human …


Open System Metabolism Of The Grand River From Headwaters To Mouth, Colin J. Assenmacher Dec 2021

Open System Metabolism Of The Grand River From Headwaters To Mouth, Colin J. Assenmacher

Honors Projects

The Grand River is the longest river in Michigan and has been greatly impacted by human activities, particularly logging in the mid-1800s, which when coupled with 20th-century urbanization and continued agricultural use, led to historically poor river health. Despite this, actions throughout the past 50 years by federal, state, and local citizen involvement, have resulted in increased river health and broader water quality monitoring within the watershed. During the summer of 2021, rapid bioassessments targeting benthic macroinvertebrates were conducted along the Grand River, with the primary goal of following up on prior state-led surveys conducted at different locations along the …


Insights Into The Role And Regulation Of The Mitochondrial Unfolded Protein Response During Pathogen Infection, Mohammed Adnan Qureshi Dec 2021

Insights Into The Role And Regulation Of The Mitochondrial Unfolded Protein Response During Pathogen Infection, Mohammed Adnan Qureshi

Biology Dissertations - Archive

Mitochondria are essential cellular organelles known for their role in energy production, apoptosis, aging, calcium signaling and others. Due to their essential nature, mitochondria are often under attack by numerous pathogens as a means to promote their infection. Consequently, mitochondrial function is often perturbed during infection, a phenomenon that must be mitigated in order for the host to survive. Cells employ various tactics to repair damaged mitochondria during stresses such as pathogenic infection. One way of countering mitochondrial stress is through the recovery mechanism known as the mitochondrial unfolded protein response (UPRmt). The UPRmt supports mitochondrial recovery by promoting mitochondrial …


Virtual Reality Marketing In Hospitality And Tourism, King-Yin Wong Dec 2021

Virtual Reality Marketing In Hospitality And Tourism, King-Yin Wong

University of South Florida (USF) M3 Publishing

OVERVIEW

In the information explosion era, consumers are now better informed about different products and services than ever. It becomes increasingly challenging for every marketer to cater to the needs of these sophisticated consumers, which is their top priority amid the fierce competition. Capitalizing on its novelty and experiential nature, virtual reality (VR) is one of the latest technologies shown to have been effective in attracting and engaging consumers, particularly in the tourism industry. A complete understanding of VR technology and its strengths and weaknesses is conducive to a better assessment of the technological tool for marketers to determine the …


Livelihood And Happiness In A Resource (Natural And Cultural)-Rich Rural Municipality In The Philippines, Rosalina Palanca-Tan, Sheila Bayog Dec 2021

Livelihood And Happiness In A Resource (Natural And Cultural)-Rich Rural Municipality In The Philippines, Rosalina Palanca-Tan, Sheila Bayog

Economics Department Faculty Publications

This paper looks at the economic and welfare conditions of residents in Lake Sebu, a largely rural but natural and cultural resource-rich municipality in Southern Mindanao in the Philippines. Two notions of welfare are used in the study: economic welfare, measured in terms of household income and vulnerability to hunger; and social welfare, measured in terms of self-reported happiness. The study uses primary data collected through a household survey and analyzed with statistical and econometric procedures (tests of difference between sub-populations; and ordinary least squares, binary probit, and ordered logistic regressions). The results suggest mixed implications of abundant natural and …


Preclinical Development Of Epicertin, A Novel Biotherapeutic For The Treatment Of Ulcerative Colitis., Micaela Ann Reeves Dec 2021

Preclinical Development Of Epicertin, A Novel Biotherapeutic For The Treatment Of Ulcerative Colitis., Micaela Ann Reeves

Electronic Theses and Dissertations

Epicertin (EPT) is a recombinant variant of the cholera toxin B subunit with a C-terminal KDEL endoplasmic retention motif. Previously, orally administered EPT demonstrated mucosal healing activity in acute and chronic dextran sulfate sodium colitis models, indicating its therapeutic potential for ulcerative colitis (UC) treatment. However, oral dosing with EPT requires neutralization of gastric acid prior to administration, hindering its facile application in the treatment of chronic diseases like UC. One solution to this issue is that EPT may also be administered intracolonically without losing efficacy. This work describes the determination of a target intracolonic dose of EPT for future …


Pyridine Nucleotide Redox Potential In Coronary Smooth Muscle Couples Myocardial Blood Flow To Cardiac Metabolism., Marc Matthew Dwenger Dec 2021

Pyridine Nucleotide Redox Potential In Coronary Smooth Muscle Couples Myocardial Blood Flow To Cardiac Metabolism., Marc Matthew Dwenger

Electronic Theses and Dissertations

The maintenance of myocardial oxygen supply during stress is essential for sustaining cardiac health. Enhancement of coronary blood flow upon increases in myocardial oxygen demand (i.e., hyperemia) relies on regulation of voltage-gated K+ (Kv) channels by their intracellular β subunits (i.e., Kvβ proteins). Considering that, Kvβ proteins are aldo-keto reductases (AKRs) and regulate Kv channel gating, we tested the hypothesis that elevation of myocardial oxygen demand modifies intracellular NAD(H) in arterial myocytes. Furthermore, we tested whether the resultant change in the redox state of the pyridine nucleotide pool directly regulates coronary Kv1 channel activity. High-resolution imaging mass spectrometry and …


Investigations Of Potential Mechanisms Underlying Spinal Cord Injury-Induced Polyuria., Jason H. Gumbel Dec 2021

Investigations Of Potential Mechanisms Underlying Spinal Cord Injury-Induced Polyuria., Jason H. Gumbel

Electronic Theses and Dissertations

Spinal cord injury (SCI) results in neurological impairments including motor, sensory, and autonomic dysfunction. These neurological deficits result in a litany of complications apart from muscular paralysis, including bladder, bowel, cardiovascular, and sexual function. SCI-induced polyuria (the overproduction/passage of urine) remains understudied, and therefore mechanisms behind it are largely unknown and require extensive investigation for potential targeted therapies to improve quality of life. The objective of this dissertation was to investigate potential mechanisms of SCI-induced polyuria and explore potential therapies to improve quality of life in the SCI population. Metabolic cages, Western blot, enzyme-linked immunoassay, and immunostaining were first used …


Exploring Service Provider Perspectives On Facilitators And Barriers To Needle Exchange Program Participation By Females Who Inject Drugs., Tammi Alvey Thomas Dec 2021

Exploring Service Provider Perspectives On Facilitators And Barriers To Needle Exchange Program Participation By Females Who Inject Drugs., Tammi Alvey Thomas

Electronic Theses and Dissertations

As the opioid epidemic lingers on across the country, many areas have set up harm reduction strategies such as needle exchange programs (NEPs) to combat the long-term consequences of injection drug use (IDU). Males and females face a plethora of health issues associated with injection drug use such as human immunodeficiency virus (HIV) and hepatitis C. While males comprise the largest portion of the injection drug use population, most research is gender neutral, which makes it difficult to discern issues specifically related to females inhibiting our ability to design interventions and procedures targeted to address their needs. Females require varying …


Impact Of Pank1 Deletion On Mitochondrial Acetylation And Cardiac Function During Pressure Overload., Timothy N. Audam Dec 2021

Impact Of Pank1 Deletion On Mitochondrial Acetylation And Cardiac Function During Pressure Overload., Timothy N. Audam

Electronic Theses and Dissertations

Recent studies have associated elevated protein acetylation levels with heart failure in humans. Although mechanisms promoting elevated acetylation levels are not fully known, excess acetyl-CoA may drive enzyme-independent acetylation of cardiac proteins. Accumulation of acetyl-CoA depends on the availability of sufficient CoA, whose production is regulated by pantothenate kinases in the CoA biosynthetic pathway. We show that cardiac proteins are hyperacetylated during heart failure in humans and tested in mice whether limiting CoA abundance would improve ventricular remodeling during pressure overload-induced hypertrophy. We limited cardiac CoA levels by deleting the rate-limiting enzyme in CoA biosynthesis, Pank1 (one of three PANK-encoding …


Assessing The Role Of Arsenic Exposure And Mirna-186 In Skin Tumorigenesis And Chromosomal Instability., Angeliki Lykoudi Dec 2021

Assessing The Role Of Arsenic Exposure And Mirna-186 In Skin Tumorigenesis And Chromosomal Instability., Angeliki Lykoudi

Electronic Theses and Dissertations

Chronic arsenic exposure through drinking water is a global health issue, affecting more than 200 million people. Arsenic is a group I human carcinogen and causes chromosomal instability (CIN). Arsenic exposure is the second most cause of skin cancer after UV radiation. MiR-186 is overexpressed in arsenic-induced squamous cell carcinoma relative to premalignant hyperkeratosis. Predicted targets of miR-186 are cell cycle regulators. Thus, we hypothesize that miR-186 overexpression drives malignant transformation of HaCaT cells by induction of CIN. Stable clones of HaCaT transfected with pEP-miR-186 expression vector or empty vector were maintained under puromycin selection were exposed to 0 or …


Characterizing The Role Of Macrophages In Cisplatin-Induced Kidney Injury And Progression To Chronic Kidney Disease., Sophia M. Sears Dec 2021

Characterizing The Role Of Macrophages In Cisplatin-Induced Kidney Injury And Progression To Chronic Kidney Disease., Sophia M. Sears

Electronic Theses and Dissertations

Cisplatin is a commonly used chemotherapeutic for treatment of many solid-organ cancers. Unfortunately, 30% of patients treated with cisplatin develop acute kidney injury (AKI), and even patients who do not develop AKI are at risk for long term declines in kidney function and development of chronic kidney disease (CKD). While traditional rodent toxicity studies have utilized a single, lethal dose of cisplatin, new models of cisplatin-induced kidney injury have revealed that repeated, low doses of cisplatin lead to development of kidney fibrosis. This model can be used to examine AKI-to-CKD transition processes. C57BL/6 mice are one of the most used …


Towards Long Term Colloid Suspension In A Vertically Rotated System., Md Mahmudur Rahman Dec 2021

Towards Long Term Colloid Suspension In A Vertically Rotated System., Md Mahmudur Rahman

Electronic Theses and Dissertations

Within a colloidal suspension gravity may compromise the observation of governing physical interactions, especially those that are weak and/or take significant time to develop. Conducting the experiment in a long-term microgravity environment is a viable option to negate gravitational effects, though significant resources are required to do so. While it may not be possible to simulate long-term microgravity terrestrially, particles can resist quick sedimentation in a confined suspension system rotating vertically with appropriate rotation speed. The goal of the investigation is to demonstrate the existence of long-term particle suspension regime for a certain colloidal suspension while characterizing colloidal behavior due …


The Role Of Sphingolipids In Aki And The Progression To Ckd: Potential Therapuetic Targets., Nicholas A. Hoffman Dec 2021

The Role Of Sphingolipids In Aki And The Progression To Ckd: Potential Therapuetic Targets., Nicholas A. Hoffman

Electronic Theses and Dissertations

Acute Kidney Injury (AKI) is most simply defined as a rapid decline in kidney function over a period of hours to days. There is currently a lack of effective treatment options for patients with AKI, highlighting the need to identify new therapeutic targets. Sphingolipids play a number of roles in different models of AKI, suggesting they could be promising future targets for treating kidney injury. Specifically, sphingosine-1-phosphate (S1P) and its receptors (S1PRs) have been implicated in numerous inflammatory disorders and models of AKI. The purpose of this review is to better characterize the role of S1P receptors in models of …