Digital Commons Network^™

Early Antibiotic Exposure Is Not Detrimental To Therapeutic Effect From Immunotherapy In Hepatocellular Carcinoma, Petros Fessas, Muntaha Naeem, Matthias Pinter, Thomas U Marron, David Szafron, Lorenz Balcar, Anwaar Saeed, Tomi Jun, Sirish Dharmapuri, Anuhya Gampa, Yinghong Wang, Uqba Khan, Mahvish Muzaffar, Musharraf Navaid, Pei-Chang Lee, Anushi Bulumulle, Bo Yu, Sonal Paul, Neil Nimkar, Dominik Bettinger, Hannah Hildebrand, Yehia I Abugabal, Tiziana Pressiani, Nicola Personeni, Naoshi Nishida, Masatoshi Kudo, Ahmed Kaseb, Yi-Hsiang Huang, Celina Ang, Anjana Pillai, Lorenza Rimassa, Abdul Rafeh Naqash, Elad Sharon, Alessio Cortellini, David J Pinato

Faculty, Staff and Students Publications

BACKGROUND AND RATIONALE: Immune checkpoint inhibitor (ICI) therapy is an expanding therapeutic option for hepatocellular carcinoma (HCC). Antibiotics (ATB) taken prior to or early during ICI therapy can impact immunotherapy efficacy across indications; however, the effect of ATB is undefined in HCC.

METHODS: In a large international cohort of 450 ICI recipients from Europe, North America, and Asia, we categorized patients according to timing of ATB focusing on exposure within -30 to +30 days from ICI (early immunotherapy period [EIOP]). EIOP was evaluated in association with overall survival (OS), progression-free survival (PFS), and best radiologic response using RECIST 1.1 criteria. …

Improving Phytoremediation Of Oil Polluted Soil In The Uae Using Acc Deaminase Producing Bacteria Under Arid Conditions, Maitha Mubarak Almansoori

Theses

Many 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase-producing and oil-degrading rhizosphere actinobacteria were isolated from oil-contaminated soils in the United Arab Emirates. These actinobacterial isolates were further selected based on their ability to solubilize phosphorus, to produce siderophores, and to produce plant growth regulators (PGRs) including auxins and polyamines. The ability of the most-promising isolate #20 (Streptomyces sp.) to promote the growth of the hyper-accumulating plants “Bermuda grass” in soil polluted with Light Arabian Crude Oil (LACO) was evaluated under greenhouse conditions. Under controlled greenhouse conditions, the application of the strongest Streptomyces sp. #20 which exhibited the maximum production of ACC deaminase and …

Neurotoxicological Effects Of Chlorpyrifos And Deltamethrin On Dopamine And Acetylcholine Systems In Drosophila Melanogaster, Hala Husam Abdul Baki

Theses

Emerging evidence suggests a positive association between pesticide exposure and sporadic Parkinson's disease (PD) development. The molecular mechanisms of PD and other neurodegenerative diseases are not fully understood, which hinders the development of therapeutic agents to cure or prevent the development of such diseases. Drosophila has been widely used as a model organism to study various neurodegenerative diseases and to screen for promising therapeutic agents. The aims of this study were: (i) investigating the toxic effect of 24 hours exposure to chlorpyrifos (CPF) and deltamethrin (DLM) on the dopaminergic system and AChE activity in Drosophila melanogaster; (ii) comparing different methodologies …

Response Of Five Chenopodium Quinoa Varieties To Different Salinity Levels, Elevated Co₂ And Uvb, Saif Ali Matar Al Blooshi

Theses

Ecosystems have been affected by climate changes. Both agriculture and environmental changes are correlated with various features since climate change is the main cause of abiotic and biotic stress which affects crop plants. The climate changes and their severe impact on plant productivity showed great intensities due to the effects of abiotic stress. In the present investigation, five quinoa varieties viz KAUST- 05395/CHFN-68 (V1), KAUST-05398/PI-614889 (V2), KAUST-05397/PI-614885 (V3), KAUST-05403/ICBA-Q3 (V4), and KAUST-05399/PI-614888 (V5) were screened for their salinity stress response by measuring the morphological parameters such as total plant height, fresh and dry weight of shoot and roots. V4 and …

Biological Treatment Of Produced Water Using Algae: A Proof Of Concept, Shibin Hashem Mohammed Nadersha

Theses

Produced water (PW) is the effluent generated during oil mining and extraction. On average, for every barrel of oil, 4 - 5 barrels of PW are generated worldwide. In UAE, 1.22 billion cubic meters of PW were generated in the year 2017. Proper management of PW is thus very important taking into account the large volumes of it being generated. In addition, PW is considered toxic as it contains various contaminants such as aliphatic and aromatic hydrocarbons, inorganic salts, metals, phenols, radioactive materials, and chemical additives. Disposal of untreated PW into oceans and water bodies can cause adverse effects on …

The Effect Of Natural Elicitors And Cold Storage Period On Quality Improvement Of Uae Date Palm Fruits (Phoenix Dactylifera, Cv. Barhi), Fatima Yaaqoub Yousef Al Shaibani

Theses

Date palm is an agricultural crop of economic importance in most Arab countries. Among the different varieties of date palms grown in the United Arab Emirates, the Barhi variety is extremely popular and is often consumed at the bisr stage (first edible stage). However, the major challenge for this fruit at bisr stage is to maintain its quality over a long period after harvest and during marketing. The objective of this study was to evaluate the synergistic effect of preharvest spraying with a natural elicitor, namely chitosan (Ch) 1%, alone and in combination with salicylic acid (SA) 2 mM and …

Abiotic Stress Effect On Growth And Photosynthetic Characteristics In Emirati Date Palm Varieties, Nasser Abdullah Ghdayer Al Kaabi

Theses

Plants are naturally exposed to various environmental stresses that affect their growth and development. As a desert plant, date palm has developed strategies to protect itself from most abiotic stresses. However, projected climate changes and the interaction between the various abiotic stressors will have profound effects on date palm adaptation and production. In the present study, five date palm cultivars, namely Chichi, Kalas, Nabt Saif, Sultana and Zamli, were initially evaluated for salinity tolerance by measuring the growth parameters such as plant height, fresh weight and dry weight of shoots and roots. Based on the obtained results, Sultana and Zamli …

Impact Of Date-Pit Activated Carbon In Different Applications: Biomedical And Environment, Betty Titus Mathew

Dissertations

Activated carbon (AC) or activated charcoal are black carbonaceous porous solid material with prominent physico-chemical properties such as low volume pore size distribution, high specific surface area, and high-degree of surface reactivity for adsorption and other chemicals reactions. AC as strong and reliable adsorbent have been reported to be employed in diverse applications stretching from water/air purification, medical, sewage treatment, energy storage devices, catalyst, supercapacitor electrodes fabrication, and as biosensors component. In this current work, AC was produced from date pits, which is a common biological waste product in UAE, and used for biomedical and environmental applications instead of using …

Impact Of E-Resources Literacy On E-Resources Consumption Habits Among College Students: An Experimental Study, Gervasius Nongkseh Mr., Moses Mark Naga Professor, Kamaljit Chirom Dr

Library Philosophy and Practice (e-journal)

E-resources and its usages are escalating rapidly today. This study is an attempt to assess the consumption of e-resources and the impact of e-literacy on the college students of Shillong. For this an experimental method was adopted. Students were given guidance on e-resources and the data was collected on different stages using a structured questionnaire to see the impact on their consumption habits. One premier institute, that is, St. Anthony’s College was selected for the study. It is spectacular to find that there is a significant difference in e-resources consumption among college students at the beginning and at the end …

Drivers Of Transcriptional Variance In Human Intestinal Epithelial Organoids, Zachary K Criss, Nobel Bhasin, Sara C Di Rienzi, Anubama Rajan, Kali Deans-Fielder, Ganesh Swaminathan, Nabiollah Kamyabi, Xi-Lei Zeng, Harsha Doddapaneni, Vipin K Menon, Deepavali Chakravarti, Clarissa Estrella, Xiaomin Yu, Ketki Patil, Joseph F Petrosino, James C Fleet, Michael P Verzi, Sylvia Christakos, Michael A Helmrath, Sumimasa Arimura, Ronald A Depinho, Robert A Britton, Anthony W Maresso, K Jane Grande-Allen, Sarah E Blutt, Sue E Crawford, Mary K Estes, Sasirekha Ramani, Noah F Shroyer

Faculty, Staff and Students Publications

Human intestinal epithelial organoids (enteroids and colonoids) are tissue cultures used for understanding the physiology of the human intestinal epithelium. Here, we explored the effect on the transcriptome of common variations in culture methods, including extracellular matrix substrate, format, tissue segment, differentiation status, and patient heterogeneity. RNA-sequencing datasets from 276 experiments performed on 37 human enteroid and colonoid lines from 29 patients were aggregated from several groups in the Texas Medical Center. DESeq2 and gene set enrichment analysis (GSEA) were used to identify differentially expressed genes and enriched pathways. PERMANOVA, Pearson's correlation, and dendrogram analysis of the data originally indicated …

Mortality, Kidney Failure, And Hospitalization Among Medicare Beneficiaries With Ckd And Pulmonary Hypertension, Sankar D Navaneethan, Carl P Walther, L Parker Gregg, Shweta Bansal, Wolfgang C Winkelmayer, Vijay Nambi, Jingbo Niu

Faculty, Staff and Students Publications

RATIONALE & OBJECTIVE: Pulmonary hypertension (PH) is highly prevalent among patients with chronic kidney disease (CKD) not requiring kidney replacement therapy. We studied the associations of PH with mortality, kidney failure, as well as cardiovascular (CV) and non-CV hospitalization among Medicare beneficiaries with a CKD diagnosis.

STUDY DESIGN: Retrospective, observational study using a matched cohort design.

SETTING & PARTICIPANTS: Patients with PH (based on 2 claims within 2 years) and patients without PH matched on CKD stage from the Medicare 5% CKD sample (1996-2016).

PREDICTOR: Presence of pulmonary hypertension.

OUTCOME: Mortality, kidney failure, and all-cause, CV, and non-CV hospitalization.

ANALYTICAL …

Microbial Dark Matter: Culturing The Uncultured In Search Of Novel Chemotaxonomy, Sarah J. Kennedy

USF Tampa Graduate Theses and Dissertations

Although <1% of environmental isolates can be cultured, insights into the culturable fraction refine future techniques to unlock the cryptic biodiversity. This cryptic biodiversity is omnipresent within culture-independent surveys of environmental bacterial populations. Subsequently, these elusive organisms are termed “microbial dark matter” due to the intrigue surrounding their potential taxonomic and biosynthetic impacts. To this end of investigating bacterial-specific dark matter, we employed high-throughput culturing techniques to examine Gulf of Mexico sediments and sponges for chemotaxonomically important bacteria. Through stepwise implementation of permissive and selective recovery techniques, we succeeded in recovering over 150 unique bacterial isolates. We observed several isolates that pose as chemotaxonomically interesting, including several strains that belong to critical bioremediation taxa. In addition to these biotechnological-amenable isolates, we have also succeeded in culturing taxa historically attributed with antibacterial discoveries. One such isolate was a previously sponge-associated organism belonging to the rarely cultured Verrucosispora genus. Through extensive comparative genomics, we identified it as a novel species and subsequently named it Verrucosispora sioxanthis sp. nov. with the published genome serving as a resource for continued microbial dark matter investigations. Following continued passaging of the organism, we observed improved growth, biomass, and liquid growth dispersal within each increasingly sub-passaged generation of the isolate. As such, the laboratory attenuation, or domestication, of the organism warranted in-depth whole genomic analyses to generate insight into the increased laboratory sustainability. Through phenotypically-guided bioinformatics, we determined that the domestication was mediated through genome-wide selective pseudo- or non-pseudogenization of genes. As the previously sponge-associated organism evolved with the selection pressures of the static laboratory, we demonstrate that isolate effectively underwent genomic atrophy to resolve into a largely stable, sustainable laboratory-domesticated strain. Collectively, our chemotaxonomic discovery pipeline expands on the culturable bacterial biodiversity and ultimately provides a previously uncultured model organism for initial forays into resolving the microbial dark matter knowledge gap.

Learning, Planning For, And Reflecting On Teaching Mathematics For Social Justice: A Case Study Investigation Of A High School Mathematics Methods Course, Queshonda J. Kudaisi

USF Tampa Graduate Theses and Dissertations

While much is known about learning to teach for social justice in other content areas, less is known about how mathematics education students learn to teach mathematics for social justice, specifically at the high school level. Hence, this mixed-methods convergent case study addresses this gap in the research literature by examining and describing the experiences of mathematics education students learning to teach mathematics for social justice within the context of a high school mathematics methods course at a large research university in the southeastern United States. The data sources accessed for this study include a survey measuring beliefs about teaching …

Workshops Mise En Place: Working With Campus Partners To Cook Up Tech Workshops In The Library, Nancy R. Curtis, Grace Liu, Anne Marie Engelsen

Faculty Research, Scholarly, and Creative Activity

No abstract provided.

Mitophagy In Depression: Pathophysiology And Treatment Targets, Ashutosh Tripathi, Giselli Scaini, Tatiana Barichello, João Quevedo, Anilkumar Pillai

Faculty, Staff and Student Publications

Mitochondria, the 'powerhouse' of eukaryotic cells, play a key role in cellular homeostasis. However, defective mitochondria increase mitochondrial ROS (mtROS) production and cell-free mitochondrial DNA (mtDNA) release, leading to increased inflammation. Mitophagy is a vital pathway, which selectively removes defective mitochondria through the process of autophagy. Thus, an impairment in the mitophagy pathway might trigger the gradual accumulation of defective mitochondria. Accumulating evidence suggest that inflammation and mitochondrial dysfunction are linked to the pathogenesis of depression. In this article, we have reviewed the role of impaired mitophagy as a contributing factor in depression pathophysiology. Further, we have discussed the potential …

Analysis Of Covid-19 Infection And Mortality Among Patients With Psychiatric Disorders, 2020, Antonio L Teixeira, Trudy Millard Krause, Lopita Ghosh, Lokesh Shahani, Rodrigo Machado-Vieira, Scott D Lane, Eric Boerwinkle, Jair C Soares

Faculty, Staff and Student Publications

IMPORTANCE: People with major psychiatric disorders are more likely to have comorbidities associated with worse outcomes of COVID-19. This fact alone could determine greater vulnerability of people with major psychiatric disorders to COVID-19.

OBJECTIVE: To assess the odds of testing positive for and mortality from COVID-19 among and between patients with schizophrenia, mood disorders, anxiety disorders and a reference group in a large national database.

DESIGN, SETTING, AND PARTICIPANTS: This cross-sectional study used an electronic health record data set aggregated from many national sources in the United States and licensed from Optum with current and historical data on patients tested …

Effects Of Drying Process On The Volatile And Non-Volatile Flavor Compounds Of Lentinula Edodes, Lijia Zhang, Xiaobo Dong, Xi Feng, Salam A. Ibrahim, Wen Huang, Ying Liu

Faculty Research, Scholarly, and Creative Activity

In this study, fresh Lentinula edodes was dehydrated using freeze-drying (FD), hot-air drying (HAD), and natural drying (ND), and the volatile and non-volatile flavor compounds were analyzed. The drying process changed the contents of eight-carbon compounds and resulted in a weaker “mushroom flavor” for dried L. edodes. HAD mushrooms had higher levels of cyclic sulfur compounds (56.55 µg/g) and showed a stronger typical shiitake mushroom aroma than those of fresh (7.24 µg/g), ND (0.04 µg/g), and FD mushrooms (3.90 µg/g). The levels of 5′-nucleotide increased, whereas the levels of organic acids and free amino acids decreased after the drying process. …

Silica Hydride: A Separation Material Every Analyst Should Know About, Joseph J. Pesek, Maria T. Matyska

Faculty Research, Scholarly, and Creative Activity

This review describes the development, special features and applications of silica hydridebased stationary phases for HPLC. The unique surface of this material is in contrast to ordinary, standard silica, which is the material most frequently used in modern HPLC stationary phases. The standard silica surface contains mainly silanol (Si-OH) groups, while the silica hydride surface is instead composed of silicon-hydrogen groups, which is much more stable, less reactive and delivers different chromatographic and chemical characteristics. Other aspects of this material are described for each of the different bonded moieties available commercially. Some applications for each of these column types are …

The Effects Aerobic Fitness Has On Heart Rate Responses For A Custody Assistant Recruit Class Performing A Formation Run, Karly Rodas, Matthew Moreno, Ashley Bloodgood, J. Jay Dawes, Joseph Dulla, Robin Marc Orr, Robert G. Lockie

International Journal of Exercise Science

International Journal of Exercise Science 14(4): 1219-1233, 2021. This study measured the heart rate (HR) responses to a formation run (group run completed along a set route) performed by higher fitness (HF), moderate fitness (MF), or lower fitness (LF) custody assistant (CA) recruits. Retrospective data from 26 recruits (12 males, 14 females) were analyzed. Prior to academy training, a YMCA step test was administered. Recruits were divided into three groups based on recovery HR: top 25% were HF; bottom 25% were LF; the rest were MF. Recruit HR was measured during a formation run completed at an ~11-minute mile· pace …

Forage Quality, Yield And Palatability Of Quackgrass (Elytrigia Repens (L.) Nevski), T. Yagi, R. Meguro, E. Fukuda

IGC Proceedings (1977-2023)

Quackgrass (Elytrigia repens (L.) Nevski) is a competitive perennial invader of pastures and hay meadows which is frequently harvested as forage in mixtures with desired forage species. Field experiments were conducted to compare quackgrass with cool-season perennial grasses grown under the same soil and climatic conditions, in terms of forage quality, productivity, and palatability. The forage quality of the hays was influenced by the grass species. Quackgrass showed forage crude protein (CP) concentration that was equal to those of perennial ryegrass (Lolium perenne), reed canarygrass (Phalaris arundinacea) and Kentucky bluegrass (Poa pratensis), and …

Supplementation With Brosimum Alicastrum Swartz To Pelibuey Sheep Fed Low Quality Rations, Luis Ramírez-Avilés, V. Valdivia-Salgado, Juan C. Ku-Vera

IGC Proceedings (1977-2023)

The objective of the present study was to assess the influence of supplementing increasing levels of ramón (Brosimum alicastrum) foliage to Pelibuey sheep fed guinea grass (Panicum maximum) hay. Rate and extent of rumen degradation of organic matter (OM) and crude protein (CP) of ramón foliage were high. Dry matter (DM) intake of the diet was increased by the inclusion of ramón foliage. However, rate and extent of digestion of guinea grass was not affected by the ramón foliage. Rate of passage of solid was linearly increased as a result of the inclusion of ramón foliage …

A Study Of Interests And Science Process Skills, Retni Sulistiyoning Budiarti, Dwi Agus Kurniawan, Putri Ayu Rivani

Jurnal Inovasi Pendidikan IPA

This study aims to determine the differences and the relationship between interest and science process skills with 80 female students and 56 male students. This research uses quantitative methods so that this research is more systematic and objective. The sampling technique used in this study used simple random sampling. Analysis of the data used using hypotheses in the form of normality, linearity and homogeneity tests and assumption tests in the form of T test and correlation test. From the results of the T test and correlation test, it was found that there were differences and relationships between the sexes in …

Healthy Eating And Physical Activity Among New Graduate Nurses: A Qualitative Study Of Barriers And Enablers During Their First Year Of Clinical Practice, Elizabeth Brogan, Chris Rossiter, Christine Duffield, Elizabeth Denney-Wilson

Research outputs 2014 to 2021

Background: New graduate nurses entering the workforce experience numerous barriers to maintaining a healthy lifestyle including shift work, the high cost of healthy foods at the workplace and high levels of exhaustion which reduce motivation to participate in regular physical activity. Research has documented unhealthy lifestyles among nurses across the profession. However, few studies focus on graduates' experiences during their transition into their careers. Aim: To investigate the barriers and enablers to healthy eating and participation in physical activity for new graduate nurses during their first year of clinical practice, and to explore attitudes to participation in workplace health promotion …

Musculoskeletal Responses To Exercise Plus Nutrition In Men With Prostate Cancer On Androgen Deprivation: A 12-Month Rct, Jack Dalla Via, Patrick J. Owen, Robin M. Daly, Niamh L. Mundell, Patricia M. Livingston, Timo Rantalainen, Stephen J. Foulkes, Jeremy L. Millar, Declan G. Murphy, Steve F. Fraser

Research outputs 2014 to 2021

Purpose: Androgen deprivation therapy (ADT) for prostate cancer has multiple adverse effects on musculoskeletal health. This 12-month randomized controlled trial aimed to assess the effects of multicomponent exercise training combined with whey protein, calcium and vitamin D supplementation on bone mineral density (BMD), structure and strength, body composition, muscle strength, and physical function in ADT-treated men.

Methods: Seventy ADT-treated men were randomized to exercise plus supplementation (Ex + Suppl; n = 34) or usual care (control; n = 36). Ex + Suppl involved thrice weekly progressive resistance training plus weight-bearing impact exercise with daily multinutrient supplementation. Primary outcomes were DXA …

Aqueous Formulations Of 1h-Cyclopropabenzene Modulate Ethylene Production And Fruit Quality In Japanese Plums, Poe N. Kyaw, Zora Singh, Vijay Y. Tokala

Research outputs 2014 to 2021

The efficacy of aqueous formulations of 1H-cyclopropabenzene (BC) containing different adjuvants to retard ethylene production and maintain fruit quality of Japanese plums (Prunus salicina Lindl. cvs. ‘Angeleno’, ‘Fortune’ and ‘Tegan Blue’) following 25 d and 40 d cold storage (1 °C) was evaluated. Plum fruit were sprayed with different solutions of 2 μM BC (i.e., aqueous solutions containing distilled water only or 5 % ethanol or 0.02 % Tween® 20 or 5 % β-cyclodextrin) or fumigated with 1 μM BC at ambient temperature. Plum fruit without any treatment were regarded as control. Regardless of the cultivars tested, all formulations of …

Hotel Complex – Case Study The Town Of Deçan, Ardit Shehu, Lulzim Beqiri

UBT International Conference

The hotel industry is at the heart of the hospitality system. This comes from the oldest traditions in the history of mankind - respect for the guest, celebration of his reception and service. It organically and naturally connects restaurants, cafes, transport and excursions into a single chain of tourist and excursion services. Today, the hotel industry as a kind of economic activity involves the provision of services and the organization of short stays in hotels, motels, camps and other accommodation facilities for a fee and represents the most powerful economic system in a region or tourist center. In our country, …

Analysis Of Anthocyanins In Cornelian Cherry (Cornus Mas L.), Hyrie Koraqi

UBT International Conference

This study aimed to determine and analyze the total anthocyanin content in extracts from cornelian cherry (Cornus mas L.). The fresh fruits of cornelian cherry (Cornus mas L.) were extracted with the solvent of EtOH 96% /0.1% HC l (1:1, ratio) at room temperature for 4 h in the dark. The extraction process was an important step in the isolation and identification of anthocyanins. For this purpose, the total anthocyanin contents in cornelian cherry (Cornus mas L.) fruits was performed with a variety of pH and analyzed by UV-Vis Spectroscopy. The results showed that the total anthocyanin content (expressed as …

A Review On Active Food Packing As Innovation Strategies For The Future, Violeta Lajqi, I. Malollari, Hyrie Koraqi, J. Llupa

UBT International Conference

Due to the dynamic life, consumers are constantly seeking to increase the shelf life of packaged food products, encouraging scientists in various fields to develop innovative packaging that meets the demands of consumers and at the same time producers. The purpose of this paper is to review the latest trends in the development of active packaging (AP) for foods. Given the specific properties of each type of food as well as the storage and distribution conditions, the choice of packaging material with accompanying elements requires a careful approach to each type of food. The knowledge of packaging materials, food properties, …

International Conference Agriculture Food Science And Technology, University For Business And Technology (Ubt) In Kosovo

UBT International Conference

UBT Annual International Conference is the 9th international interdisciplinary peer reviewed conference which publishes works of the scientists as well as practitioners in the area where UBT is active in Education, Research and Development. The UBT aims to implement an integrated strategy to establish itself as an internationally competitive, research-intensive university, committed to the transfer of knowledge and the provision of a world-class education to the most talented students from all background. The main perspective of the conference is to connect the scientists and practitioners from different disciplines in the same place and make them be aware of the recent …

International Conference On Pharmaceutical And Natural Sciences, University For Business And Technology - Ubt

UBT International Conference

UBT Annual International Conference is the 9th international interdisciplinary peer reviewed conference which publishes works of the scientists as well as practitioners in the area where UBT is active in Education, Research and Development. The UBT aims to implement an integrated strategy to establish itself as an internationally competitive, research-intensive university, committed to the transfer of knowledge and the provision of a world-class education to the most talented students from all background. The main perspective of the conference is to connect the scientists and practitioners from different disciplines in the same place and make them be aware of the recent …