Digital Commons Network^™

Probing The Role Of Astrocytes In The Pathology Of Fragile X Syndrome With Human Stem Cells, Baiyan Ren

Theses & Dissertations

Fragile X syndrome (FXS) is an X-linked neurodevelopmental disorder related to intellectual disability and the most common monogenic cause of autism spectrum disorder. FXS is mainly caused by an expansion of CGG repeats in the 5’-untranslated region of fragile X mental retardation 1 (FMR1) gene, leading to the loss of expression of fragile X mental retardation protein (FMRP). Astrocytes are the most abundant glial cells in the central nervous system (CNS). Loss of FMRP in astrocytes has been found to contribute to structural and functional synaptic deficits in the Fmr1-KO mouse model. The contribution of human astrocytes, however, to the …

Glad To Be Out. I Was Running Out Of Womb: A Study Of Omega-6 And Omega-3 Fatty Acid Metabolites At Delivery And Their Relationship To Infant Growth, Prematurity, And Admission To The Neonatal Intensive Care Unit, Maranda Thompson

Theses & Dissertations

Normal pregnancy relies on inflammation for implantation, placentation, and parturition, but uncontrolled it can lead to poor infant outcomes. Maternal diet is one modifiable factor that can impact inflammation. Omega-3 and omega-6 fatty acids have been shown to be metabolized into bioactive metabolites that effect inflammation. The presence and importance of these metabolites at the time of delivery are not well understood. Therefore, the purpose of this dissertation was to first quantify omega-3 and omega-6 metabolites in maternal plasma, cord plasma, and placental tissue. Then the relationships between metabolites and infant outcomes, including birth weight percentile, length percentile, head circumferences …

Functional Characterization Of Cancer-Associated Dna Polymerase Ε Variants, Stephanie R. Barbari

Theses & Dissertations

Replicative DNA polymerases ε (Polε) and δ (Polδ) achieve high fidelity DNA synthesis through a precise balance of polymerization and exonucleolytic proofreading. Errors that escape proofreading are corrected by DNA mismatch repair (MMR). Ultramutated human cancers with proficient MMR carry alterations in the exonuclease domain of Polε, which were initially predicted to abolish proofreading. However, functional studies in yeast of the most recurrent Polε-P286R variant suggested defects beyond a loss of exonuclease activity. Indeed, biochemical analysis of the yeast Polε-P286R analog revealed increased polymerization capacity in addition to decreased proofreading, which enables efficient mismatch extension and bypass of replication-blocking non-B …

Understanding The Impact Of Chlamydia Trachomatis Infection On Host Vesicular Trafficking, Lisa M. Jorgenson

Theses & Dissertations

Chlamydia trachomatis is a highly evolved obligate intracellular bacterial pathogen. During infection, organisms reside within a host epithelial cell in a membrane-bound vacuole called the inclusion. The inclusion membrane contains both pathogen and host components, including unique chlamydial type III effector proteins called Incs and host lipids like cholesterol and sphingomyelin. The inclusion membrane serves as the main barrier between the bacteria and the host cytosol, and thus, functions as the platform supporting host-chlamydial interactions. Via the inclusion membrane, C. trachomatis intercepts components of host vesicular traffic including many host SNARE proteins, which are a conserved family of eukaryotic proteins …

Immunological Factors Associated With Siv/ Shiv Persistence In Diverse Tissue Niches, Omalla A. Olwenyi

Theses & Dissertations

The significant challenge towards a successful HIV cure lies in eradicating persistent viral reservoirs across diverse tissue niches. As a result, HIV-infected individuals have to resort to lifelong antiretroviral therapy. Recent news of supposed HIV eradication in a second patient has further re-invigorated the fields of HIV cure. However, a few barriers remain, such as the lack of currently available assays to accurately quantify viral reservoirs, limited information on cellular factors associated with persistence, and varied dynamics of the viral reservoir in various body compartments. Lastly, HIV-infected individuals live different lifestyles stemming from comorbid substance abuse including consumption of morphine, …

Metabolic Heterogeneity And The Roles Of Cody And Ccpa In Central Metabolism And S. Aureus Biofilm Formation., Logan L. Bulock

Theses & Dissertations

Staphylococcus aureus is a metabolically versatile human pathogen, causing disease in many areas of the body. Its versatility can be attributed to the fact that it utilizes a variety of tools to adapt to many different environments, including toxins to scavenge from the host and multiple transporters to compete for its preferred carbon sources. S. aureus can also survive in harsh conditions through biofilm development, which are notoriously recalcitrant to antibiotics and immune defenses. Biofilms exhibit marked heterogeneity, with division of labor for production of matrix components and differential gene expression among various niches within the biofilm.

In this study, …

Ud In The News Dec. 10-17, University Of Dayton

News Releases

The Baltimore Sun, Law360 and Jewish News of Northern California interviewed Charles Russo, director of the program in educational leadership and research professor of law, about a variety of legal topics facing schools. Slate talked to Vince Miller for its story "Santa Claus: Why do Italian priests have a vendetta against Father Christmas?" Associate Professor of Accounting Sarah Webber contributed a piece to The CPA Journal about the Cohan rule, which addresses the situation when a taxpayer lacks documentation to support items on a tax return.

The Lived Experiences Of Self-Concealment Among Lgbq+ Counselors In The Workplace, Richard Charette Ii

Electronic Theses and Dissertations

This study sought to unearth the narratives of LGBQ+ counselors’ experiences of self-concealment in the workplace. Self-concealment was an identity management strategy that was defined as an active, and sometimes persistent effort to conceal LGBQ+ identity. Self-concealment of affectional identity was well researched in workplace contexts, however only one study existing study examined self-concealment among LGBQ+ counselors. In that previous qualitative inquiry, Jeffery and Tweed (2014) called for further exploration of self-concealment among LGBQ+ counselors when self-concealment emerged as a surprising finding.

The phenomenon of self-concealment was under-researched in counseling but has been extensively conceptualized as both a developmental stage …

Characterization Of Phase Behavior In Idp Solutions By Uv-Vis Spectrophotometry And Phase Volume Fraction Measurements In Capillaries, Qing Sun

Biomedical Engineering ETDs

Intrinsically disordered proteins (IDPs) play vital roles in cell signaling and regulation by changing conformation dynamically under various stimulus. Thus, it is crucial to understand their physical properties in vitro especially liquid-liquid phase separation (LLPS). Here, we first used UV-vis spectrophotometry to investigate two of IDPs' physical properties – transition temperature (Tt) and coacervate size. This deeper insight allows us to further understand the dynamics of phase growth. We then constructed a binodal curve in the phase diagram by measurement of volume ratios in phase separated IDP solution in glass capillaries.

American Lotto, Kris Parker

Capstones

The Preka family won the diversity visa lottery and has immigrated to the seaside town of New London, Connecticut. They are a family of four that have dreamed of immigrating to the United States for much of their lives. Originally from Albania, a country with limited opportunities and riddled with corruption, the film will follow them in Connecticut as they adjust to life in the US and the challenges of learning a new language, finding decent work, and adjusting to a new culture. The film explores their emotional journey; their hopes, expectations, and disappointments, as they build a life without …

Maxed Out: How The Pandemic Put The Fitness Industry’S Fate In The Hands Of Its Consumers, Denny Jacob

Capstones

The pandemic's impact on the fitness industry has gone deeper than closing gyms. It has completely reoriented how people workout, where they workout, how gyms will look and run and the new balance between brick-and-mortar gym locations and virtual fitness. The seismic shifts that have taken place over nearly two years have moved the industry to a place that it can't revert from. Everything that has changed since is here to stay in some capacity.

Link to capstone: https://djacob1670.wixsite.com/capstone

A Perfect Storm For Slashing Athletic Budgets - Covid, Coaches Salaries, And Nil, Patrick L. Elliott

Capstones

Just when college athletes were beginning to see progress with landmark NIL legislation, a new threat emerged in college athletics. After years of fighting for the right to earn compensation from their athletics talents, more college teams than ever before are being eliminated due to budget concerns with universities and athletic departments.

This has been further amplified as a result of the COVID-19 pandemic and the loss of revenue from shutdowns and cancellations in 2020. Athletes in non-revenue earning sports account for well over 90% of all NIL and they are now at risk of losing the opportunity to compete …

Diabetic Education In An Uninsured Patient Population, Amanda Whistler

Doctor of Nursing Practice Projects

Abstract

Patient education is an important factor in the management of Type 2 Diabetes. Successful outcomes can be causally related to proper patient education. Many times, patients are left with more questions than answers about their newly diagnosed condition. Some groups of patients are affected by prediabetes and diabetes more than other groups. Differences in health status or access to health care among racial, ethnic, geographic, and socioeconomic groups are referred to as health disparities. This is a major concern in the United States for health care providers.

A spreadsheet was used to record the blood glucose levels of each …

Short Term Medical Mission Antibiotic Protocol, Greg Jennings

Doctor of Nursing Practice Projects

Background/Introduction: Short term medical mission trips are growing in popularity and with this growth the level of care provided must remain evidenced based. Mission groups traveling to developing countries consist primarily of volunteers. And while many of those serving on mission trips are medically trained, some have minimal healthcare knowledge. Pharmacists are not always available to dispense medications on mission trips and therefore it is common for registered nurses in the pharmacy to dispense the medications prescribed by the providers. Because the medications available on mission trips are limited, often times substitutions must be made, especially when it comes …

Prediction Equations To Determine Induced Force On Reinforcing Elements Due To Laterally Loaded Piles Behind Mse Wall And Lateral Load Test On Dense Sand, Pedro David Garcia Montesinos

Theses and Dissertations

Researchers performed 35 full-scale lateral load tests on piles driven within the reinforcement zone of a mechanically stabilized earth wall (MSE wall). Data defining the induced tensile force on the reinforcements during lateral pile loading was used to develop multi-linear regression equations to predict the induced tensile force. Equations were developed by previous researchers that did not consider the diameter of the pile, the fixed head condition, relative compaction, or cyclic loading. The purpose of this research was to include this tensile force data and develop prediction equations that considered these variables. Additionally, a full-scale lateral load test was performed …

Increase Access To Community-Based Health Care For Underserved Individuals: A Quality Improvement Project, Piper Oneal

Doctor of Nursing Practice (DNP) Manuscripts

Abstract

Background: Health care access disproportionately affects certain populations. Health care opportunities for uninsured adult members of a rural community can be limited. Suwannee County is a rural community with a population of 43,814 of which 40.8 % live below the 200% poverty income level (WellFlorida Council, 2020). Problem: In 2016, 23.8% of adults in Suwannee County were unable to see a doctor in the past year due to the inability to pay and lack of insurance (Florida Department of Health, 2020). Community-based health care initiatives can address such issues by targeting underserved individuals, reducing barriers, and making health services …

A Survey Of Faculty Perceptions Of Community College Career And Technical Education, Thomas Gauthier Dr.

Journal of Research in Technical Careers

Community colleges are the leaders in facilitating career and technical education (CTE), and faculty help develop program offerings on campus. This study explored faculty perceptions of community college CTE programs using the survey research method. Participants included 36 faculty members from various disciplines from 15 state colleges in Florida. Participants were sent a digital survey and asked to use a scale from 0 (do not agree) to 8 (agree) to score their agreement level with 43 statements of opinion. Data revealed that community college faculty perceive CTE as beneficial, but CTE programs must include the habits of mind and support …

Developing A Structured Ojt Program: The Case Of A Small Manufacturing Company In A Rural Region, Raymond A. Dixon Ph.D.

Journal of Research in Technical Careers

This study explored the process followed by a small manufacturing company in a rural area to implement a planned training program to develop the technical skills of its engineering/production workers as the company expands its operation. A case study design was used. Data was collected over an eight-month period by the examination of documents, direct observation, and interview. Findings indicate the process followed were consistent with elements of the Talent Development Architecture. The growth of the company presents learning opportunities for both the owner and his management team. The job analysis provided inputs to implement a planned training program and …

Beyoncé: A Powerful, Black Icon, Emma Kury

Faculty Curated Undergraduate Works

Abstract

Beyoncé, one of the most powerful Black, female artists is able to use her power to share her feminist perspectives with all of her fans. Originally in Destiny’s Child, Beyoncé decided to go solo to create her own music and career. Today, Beyoncé empowers her audience with her music and extravagant performances. Although Beyoncé faces various personal challenges during her career, she is still able to attain her main goal of giving to her fans. Additionally, Beyoncé is able to pave the way for other artists who follow her by giving an example of how artists can interact with …

The Rise Of Femicide & Digital Activism, Hannah Micciolo

Faculty Curated Undergraduate Works

Women’s voices are often silenced as compared to men, especially when it comes to speaking out against the injustice women have faced. In Mexico, women had been enduring femicide for decades, oftentimes being silenced and overshadowed by the government’s narrative. In this new wave of activism called digital activism, people are now using social media to inform their audience about specific issues. Florence Pugh is one of these people. Pugh uses digital activism to spread awareness for Femicide in Mexico and encourage her audience to also speak up on the issue in a time where women’s voices are silenced.

More Than An Athlete: A Qualitative Analysis Of How Student-Athletes Develop Self-Authorship Through Their Experiences In Athletics, Haley Kate Webb

Honors Theses

Within the context of the newly instituted NCAA Name, Image and Likeness legislation, student-athletes are able to profit from their personal brands. This legislation presents newfound exposure to student-athletes. In order to assess the preparedness of student-athletes to benefit monetarily from their personal brands, an analysis of student-athlete development provides beneficial insight regarding their cognitive development. Using self-authorship theory as a guide, this qualitative research used long interviews to explore how student-athletes are developing their identities through their journeys with athletics. The research analyzes the lived experiences told by five athletes from a medium-sized faith-based NCAA Division II university. The …

Pentagonal Extensions Of The Rationals Ramified At A Single Prime, Pablo Miguel Rodriguez

Theses and Dissertations

In this thesis, we define a certain group of order 160, which we call a hyperpentagonal group, and we prove that every totally real D5-extension of the rationals ramified at only one prime is contained in a hyperpentagonal extension of the rationals. This generalizes a result of Doud and Childers (originally conjectured by Wong) that every totally real S3 extension of the rationals ramified at only one prime is contained in an S4 extension.

Who Pays To Clean Up What A Dying Industry Leaves Behind?, Audrey Carleton

Capstones

As the birthplace of oil and gas, Pennsylvania is home to the highest number of orphaned abandoned oil and gas wells in the country — an estimated 300,000 to 500,000 dot the Keystone State, all remnants of a dying industry. These wells emit methane, a potent greenhouse gas, and have, for decades, caused alarm to hundreds of residents, documents obtained in a months-long capstone investigation show. The state is currently plugging 10 to 12 abandoned wells per year, on average, out of hundreds of thousands, in part because it has not collected enough in cleanup funds from producers to fund …

Trans Sex Workers - A Community In Resilience, Alejandra Pedraza Buenahora

Capstones

By exploring the ways the trans sex worker community supports itself and all the tactics community leaders are using to support community members, this engagement journalism projects details the way journalist can service the trans community and aid in their information acquisition needs. By using an array of journalistic skills this capstone explores what journalist can and should do to build trust with trans community members to better report on the experiences of individual community members. Here you will find detailed lessons learned from interviews, informal chats, reporting and gatherings with trans community leaders in the city of New York.

Living Positive, Cheyann Harris, Emani King Mack

Capstones

A short documentary by Emani King Mack & Cheyann Harris, that focuses on Black men who are living with HIV. This film features the stories of Malik Davon, Jah Love, and Melchizedek Roberson to destigmatize, empower, and set a new precedent in media on how to cover people living with HIV.

Link to capstone: https://cheyannharris.wixsite.com/livingpositive

"Our Strength Is Unity:" Delivery Bikers In Their Own Words, Connor W. Zaft

Capstones

"Our Strength Is Unity" is a year-long photographic essay on food delivery workers and their attempts to self-organize during the pandemic.

Retail Workers On The Frontlines, Anthony L. Medina

Capstones

Retail work culture has been forever altered by COVID 19. The pandemic shook what was life in New York City. Two years later the nation continues to grapple with the impact of the virus.

Next to frontline workers, retail workers who man the cashiers and maintain the sales floors at big chain stores that sell socks, shirts, shoes and other everyday items are just as vulnerable to the physical and social impact of the pandemic and in some ways provide services just as essential to health care workers.

This photo essay shares the lives of three Brooklyn natives: Cheyann Harris, …

History Matching And Prediction Of A Polymer Flood Pilot In Heavy Oil Reservoir On Alaska North Slope, Xindan Wang

Engineering

The first-ever polymer flood pilot to enhance heavy oil recovery on Alaska North Slope is ongoing. After more than 3 years of polymer injection, significant benefit has been observed from the decrease in water cut from 65% to less than 15% in the project producers. The primary objective of this study is to develop a robust history-matched reservoir simulation model capable of predicting future polymer flood performance. In this work, the reservoir simulation model has been developed based on the geological model and available reservoir and fluid data. In particular, four high transmissibility strips were introduced to connect the injector-producer …

Filling Information Gaps In Milwaukee’S Metcalfe Park Neighborhood, Kynala J. Phillips

Capstones

While in the Craig Newmark Graduate School of Journalism’s Engagement Journalism program, I got the chance to work with residents in Milwaukee’s predominantly Black Metcalfe Park neighborhood and the organizations and people that communicate most frequently with its residents.

During these past 18 months, I have practiced deep listening and slow and engaged journalism to build trust with my community. The goal: to create a product that is useful to the residents of Metcalfe Park.

To accomplish this, I joined a small group of journalists and community leaders to produce a print and SMS neighborhood newsletter titled “North 2 Center” …

Piled Up, Sophia Lebowitz

Capstones

Piled Up is a 10 minute film about Anna Sacks, a dumpster diver in New York City who has recently found viral social media fame for calling out wasteful corporate practices. Meanwhile, the physical build-up of the trash she collects, and the anxiety of the never-ending cycles of waste are weighing her down and keeping her from her goals.

This is a surprising story about a woman who has found overwhelming social media success while simultaneously dealing with the mechanism of that success, waste, building up in her life. Her videos have garnered her thousands of followers and millions of …