Digital Commons Network^™

Molecular Detection Of Campylobacter Species From Human And Cattle Faecal Samples In Kilosa District, Tanzania, Gahamanyi Noel, Leonard E.G Mboera, Mecky I. Matee, Dieudonné Mutangana, Raghavendra G. Amachawadi, Kye-Yoon Yoon, Mr. Humphrey, Cheol-Ho Pan, Erick V.G Komba

All Rsif Scholars' Publications

A growing number of Campylobacter species other than C. jejuni and C. coli have been considered as emerging human and animal pathogens but their contribution to human gastroenteritis is poorly documented. This study aimed at detecting Campylobacter species from human and cattle faecal samples in Kilosa District, Tanzania using molecular techniques without culture. Seventy (70) faecal samples were collected from five diarrheic and 65 non-diarrheic human patients attending Kilosa District Hospital in Tanzania from July to October 2019. During the same period, 30 faecal samples were also collected from healthy cattle in the same district. Genus and species identification of …

An Assessment Of The Epidemiology And Socioeconomic Impact Of The 2019 African Swine Fever Outbreak In Ngara District, Western Tanzania, Jean Nepomuscene Hakizimana, C.C Kivumbi, C Yona, Gerald M. Misinzo

All Rsif Scholars' Publications

African swine fever (ASF) is a contagious viral transboundary animal disease affecting domestic pigs caused by ASF virus (ASFV). This study was conducted in order to determine the genetic characteristics, risk factors and socioeconomic impact of an ASF outbreak in 2019 in Ngara, western Tanzania. Tissue samples from dead domestic pigs with clinical picture suggestive of ASF infection were collected for ASF confirmation and genetic characterization of ASFV. Data on the risk factors and socioeconomic impact associated with the ASF outbreak were collected from consenting farmers using a semi-structured questionnaire. Disease confirmation was done by detection of genomic ASFV DNA …

Nanobodies: Their Potential For Applications In Biotechnology, Diagnosis And Antiviral Properties In Africa; Focus On Application In Agriculture, Faith Njeri Njeru, Paul Mbogo Kusolwab

All Rsif Scholars' Publications

Antibodies are key elements in routine immunological tests, having a long history in detection of plant pathogens due to their specificity, reliability and fast performance. Heavy chain-only antibodies (HCAbs), circulating in the blood of camels are IgG isotypes that lack the light (L) chains and the heavy (H) chain is depleted of the first constant domain (CH1). The HCAbs interact with the antigen by virtue of only one single variable domain, referred to as VHH or nanobody (Nb). Owing to their unique properties: nanometer scale size, robust structure, stable and soluble behaviour in aqueous solution, high affinity and specificity for …

Prevalence And Antimicrobial Susceptibility Profiles Of Staphylococcus Aureus From Raw Bovine Milk In Dairy And Pastoral Farms In Morogoro Region, Tanzania, Nancy Kalee, Gahamanyi Noel, Abubakar S. Hoza

All Rsif Scholars' Publications

Increased resistance of Staphylococcus aureus isolates to existing antimicrobials constitutes a major concern in human and veterinary medicine. This study aimed to determine the prevalence, antimicrobial susceptibility profiles, and molecular characteristics of S. aureus from raw bovine milk in dairy and pastoral farms in Morogoro urban and Mvomero districts, Tanzania. In a cross-sectional study, 397 raw bovine milk samples were collected and carried to the laboratory. Conventional Gram staining, colony morphology on blood agar, and mannitol salt agar, along with biochemical tests, were used for S. aureus identification. Antimicrobial susceptibility testing (AST) was performed using the disk diffusion method, while …

Elucidation Of Multi-Origin Inula Helenium L. For Antimicrobial Activity And Heavy Metal Profiling As An Exemplar For The Use And Regulation Of Plants As Antibiotics., Ciara Ruth Kenny

Theses

An impending post-antibiotic era looms, thus new sources of therapeutic chemical entities are crucial to sustain effective antimicrobial chemotherapy worldwide. Plant natural products are a source of accessible, structurally diverse compounds that provide therapeutic potential. The pharmacological applications of plants in medicine can be guided by the attestation of traditional use or the application of this knowledge to uncover new leads in the drug discovery process. The range of modified in vitro methods commonly used to investigate preclinical antimicrobial efficacy of plant-derived natural products, and the associated limitations and challenges in the provision of new antimicrobial drugs from plants is …

For The Children? A Mixed Methods Analysis Of World Bank Structural Adjustment Loans, Health Projects, And Infant Mortality In Latin America, Shiri Noy

Faculty Publications

No abstract provided.

Prevalence And Correlates Of Physical Health Conditions In Jamaica And Guyana, Krim K. Lacey, Karen Powell Sears, Tazhmoye V. Crawford, Letroy O. Cummings, Sasha R. Drummond-Lewis, Rohan D. Jeremiah, James S. Jackson

Faculty Publications

No abstract provided.

The Case For Learning History In Science Class: How Historical Context Shapes Scientific Discovery, Mia Formato

The Synapse: Intercollegiate science magazine

No abstract provided.

Uvc Automatic Door Handle Sanitizer, Dean Dalton, Tate Patterson

Electrical and Computer Engineering

Infectious disease is easily spread through the use of door handles

• A virus placed on a single door handle can spread through the remainder of the building within 2 to 4 hours
• Ultraviolet Class C light (UVC) is effective at sanitizing surfaces easily and at a low cost
• UVC light can be harmful when there are high levels of direct exposure to the eyes

Automatic Door Handle Sanitizer

• The device applies UVC light to effectively sanitize while safely allowing the use of the door

Complete Genome Sequence Of Rickettsia Parkeri Strain Black Gap, Sandor E. Karpathy, Christopher D. Paddock, Stephanie L. Grizzard, Dhwani Batra, Lori A. Rowe, David T. Gauthier

Biological Sciences Faculty Publications

A unique genotype of Rickettsia parkeri, designated R. parkeri strain Black Gap, has thus far been associated exclusively with the North American tick, Dermacentor parumapertus. The compete genome consists of a single circular chromosome with 1,329,522 bp and a G+C content of 32.5%.

Impact Of New Coronavirus Pneumonia On Chinese Import And Export Trade, Hengyu Guo

Chulalongkorn University Theses and Dissertations (Chula ETD)

Coronavirus disease 2019 or COVID-19 is an epidemic caused by a severe acute respiratory syndrome coronavirus that has led to a persistent outbreak that has become one of the terrible epidemics in human history. The epidemic has had a huge impact on the world economy and trade business. In the background of the COVID-19 pandemic, how to mitigate the negative effects of the epidemic and prevent the impact of similar diseases on import and export trade has become a major issue for the international community to address. Based on China's import trade，export trade data before the epidemic, China's import trade，export …

Safe-Haven Property Of Gold Toward Multi Asset Portfolio During Covid-19 Pandemic, Naratporn Thamthonsiri

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study test whether gold has a property of safe-haven asset during the COVID-19 pandemic by performing multivariate time series analysis against multiple asset classes (equity, fixed income, property fund and REIT, and BITCOIN). Dividing testing period into pre- (2015 -2019) and on-going COVID-19 period (2020), the result of long run cointegration test came out that gold is shown to have a cointegrating relationship only during COVID-19 and no cointegration during pre-pandemic. This can be indicated that gold does not have the property of being a hedging instrument over a long run but consider itself to be a hedging asset …

Thai Foreign Policy Amid The Us-China Competition Of Vaccine Diplomacy Between 2020 And 2021, Vajaris Hemmaphat

Chulalongkorn University Theses and Dissertations (Chula ETD)

This research seeks to analyze Thai foreign policy’s posture toward the United States and China amid the competition of vaccine diplomacy in the Southeast Asia region between 2020 and 2021. This research focuses on the conduct of Thai foreign policy by applying ‘hedging strategy’ as a main theoretical framework in explaining how Thailand managed to diversify its COVID-19 vaccine portfolios, in order to have a clearer understanding of whether Bangkok can perform a well-crafted hedging strategy or Thai’s traditional diplomacy has lost ground in its default setting. This research has been done by examining official documents from the Thai government, …

ความร่วมมือระหว่างหน่วยงานภาครัฐกับชุมชนในการรับมือกับการแพร่ระบาดของโรคติดเชื้อไวรัสโคโรนา 2019: กรณีศึกษาการจัดตั้งศูนย์ดูแลผู้ป่วยติดเชื้อโควิด19 โรงเรียนวัดจุฬามณี ตำบลบ้านกุ่ม อำเภอบางบาล จังหวัดพระนครศรีอยุธยา, อภิชญา บูรณวัฒน์

Chulalongkorn University Theses and Dissertations (Chula ETD)

งานวิจัยนี้มีวัตถุประสงค์เพื่อศึกษาความร่วมมือระหว่างหน่วยงานภาครัฐกับชุมชนในการรับมือกับการแพร่ระบาดของโรคติดเชื้อไวรัสโคโรนา 2019 กรณีศึกษาการจัดตั้งศูนย์ดูแลผู้ป่วยติดเชื้อโควิด19 โรงเรียนวัดจุฬามณี ตำบลบ้านกุ่ม อำเภอบางบาล จังหวัดพระนครศรีอยุธยา โดยใช้กรอบแนวคิดเกี่ยวกับกระบวนการมีส่วนร่วมการบริหารราชการแบบมีส่วนร่วมและการบริหารราชการแบบร่วมมือกัน ซึ่งมีลักษณะเป็นการวิจัยเชิงคุณภาพและเก็บรวบรวมข้อมูลด้วยเทคนิคการสัมภาษณ์เชิงลึกจากผู้ให้ข้อมูลสําคัญที่เกี่ยวข้องในการดำเนินงานจัดตั้งศูนย์ดูแลผู้ป่วยติดเชื้อโควิด19 ทั้งในส่วนของภาครัฐและภาคประชาชนในชุมชนตำบลบ้านกุ่ม ทั้งนี้ผลการศึกษาพบว่าในช่วงที่มีการระบาดรุนแรง หน่วยงานภาครัฐภายในพื้นที่มีความพยายามที่จะจัดตั้งศูนย์พักคอย แต่มีข้อจำกัดด้านโครงสร้าง งบประมาณ และกฎระเบียบต่างๆ ที่ทำให้การดำเนินการจัดตั้งศูนย์พักคอยมีความล่าช้า ดังนั้น ชาวบ้านในชุมชนตำบลบ้านกุ่มจึงเป็นฝ่ายเริ่มรับมือกับการแพร่ระบาดของโรคดังกล่าวโดยการคัดแยกผู้ป่วยออกจากประชาชนที่ยังไม่ติดเชื้อในชุมชน และนำไปสู่การจัดตั้งศูนย์ดูแลผู้ป่วยติดเชื้อโควิด-19 ของตำบลบ้านกุ่มขึ้นมา ทั้งนี้ศูนย์ดังกล่าวได้มีการระดมทรัพยากรทั้งในด้านการเงิน แรงงาน วัสดุอุปกรณ์ และการประสานงานกับหน่วยงานต่างๆ ตลอดจนการได้รับการสนับสนุนจากเครือข่ายทางสังคมและหน่วยงานภาครัฐที่เกี่ยวข้อง อีกทั้งภาครัฐก็ปรับบทบาทจากการเป็นหน่วยงานหลักในการรับมือกับวิกฤตดังกล่าว มาสู่การเป็นฝ่ายสนับสนุนโดยการให้คำปรึกษาและการประสานงานเกี่ยวกับการดำเนินการจัดตั้งศูนย์ ในขณะเดียวกันเมื่อพิจารณาถึงแนวทางการพัฒนาการบริหารจัดการการมีส่วนร่วมอย่างยั่งยืนในชุมชน ชุมชนดังกล่าวได้เล็งเห็นถึงความจำเป็นเกี่ยวกับการถอดบทเรียนจากการดำเนินการบริหารจัดการศูนย์ดังกล่าว ซึ่งมีลักษณะเป็นการนำหลักการการมีส่วนร่วมของชุมชน และการจัดการปกครองสาธารณะแนวใหม่ มาปรับใช้เพื่อการเตรียมการรับมือกับการแพร่ระบาดของโรคติดเชื้อไวรัสโคโรนา 2019 หรือสถานการณ์วิกฤติอื่นๆ ที่อาจเกิดขึ้นภายในชุมชนในอนาคต ทั้งนี้จากการถอดบทเรียนในเบื้องต้นพบว่าปัจจัยสำคัญที่ส่งผลให้กระบวนการการมีส่วนร่วมของชุมชนประสบผลสำเร็จ ได้แก่ ความเข้มแข็งของผู้นำและชาวบ้าน, ผู้นำและเจ้าหน้าที่ภาครัฐทุกระดับมีทัศนคติที่ดีต่อกระบวนการสร้างการมีส่วนร่วมของประชาชน, ประชาชนสามารถเข้าถึงข้อมูลข่าวสารได้อย่างถูกต้องและเพียงพอผ่านการใช้เครื่องมือในการสื่อสารอย่างมีประสิทธิภาพ, การเปิดโอกาสให้ทุกภาคส่วนที่เกี่ยวข้องเข้ามามีส่วนร่วมในการดำเนินการแก้ไขปัญหาที่เกิดขึ้นในสังคม, การมีทรัพยากรที่เพียงพอและสามารถนำทรัพยากรไปใช้ได้อย่างมีประสิทธิภาพ

Covid-19 Vaccine And Social Media In The U.S.: Exploring Emotions And Discussions On Twitter, Amir Karami, Michael Zhu, Bailey Goldschmidt, Hannah R. Boyajieff, Mahdi M. Najafabadi

Faculty Publications

The understanding of the public response to COVID-19 vaccines is the key success factor to control the COVID-19 pandemic. To understand the public response, there is a need to explore public opinion. Traditional surveys are expensive and time-consuming, address limited health topics, and obtain small-scale data. Twitter can provide a great opportunity to understand public opinion regarding COVID-19 vaccines. The current study proposes an approach using computational and human coding methods to collect and analyze a large number of tweets to provide a wider perspective on the COVID-19 vaccine. This study identifies the sentiment of tweets using a machine learning …

Uncertainty Due To Infectious Diseases And Stock–Bond Correlation, Konstantinos Gkillas, Christoforos Konstantatos, Costas Siriopoulos

All Works

We study the non-linear causal relation between uncertainty-due-to-infectious-diseases and stock–bond correlation. To this end, we use high-frequency 1-min data to compute daily realized measures of correlation and jumps, and then, we employ a nonlinear Granger causality test with the use of artificial neural networks so as to investigate the predictability of this type of uncertainty on realized stock–bond correlation and jumps. Our findings reveal that uncertainty-due-to-infectious-diseases has significant predictive value on the changes of the stock–bond relation.

Cancer Incidence, Mortality, Years Of Life Lost, Years Lived With Disability, And Disability-Adjusted Life Years For 29 Cancer Groups From 2010 To 2019: A Systematic Analysis For The Global Burden Of Disease Study 2019, Jonathan M. Kocarnik, Global Burden Of Disease 2019 Cancer Collaboration, Ali Artaman

All Works

Importance: The Global Burden of Diseases, Injuries, and Risk Factors Study 2019 (GBD 2019) provided systematic estimates of incidence, morbidity, and mortality to inform local and international efforts toward reducing cancer burden. Objective: To estimate cancer burden and trends globally for 204 countries and territories and by Sociodemographic Index (SDI) quintiles from 2010 to 2019. Evidence Review: The GBD 2019 estimation methods were used to describe cancer incidence, mortality, years lived with disability, years of life lost, and disability-adjusted life years (DALYs) in 2019 and over the past decade. Estimates are also provided by quintiles of the SDI, a composite …

Effectiveness Of Communication Program On Reduction Of Tb Stigma And Discrimination Among High-School Students In Bangkok: A Quasi-Experimental Study, Saowaluk Moonsarn

Chulalongkorn University Theses and Dissertations (Chula ETD)

Tuberculosis (TB) is a contagious disease through airborne transmission, and it is still a major cause of illness in Thailand. In addition, some patients with TB infection suffer from stigma and discrimination while living in their community. However, the stigma of TB patients is also reported to be high among high school students. The study aimed to explore the effectiveness of communication program on reduction of TB stigma and discrimination among high-school students in Bangkok. A quasi-experimental study was implemented in two high schools with 212 high-school students (Experimental group =108; Control group=104). Purposive and systematic sampling techniques were adopted …

การศึกษาการแสดงออกของ Efflux Pump ในเชื้อ Acinetobacter Baumannii ที่ถูกเหนี่ยวนำให้ดื้อยา Amikacin, ณัฐนิชา บัวงามดี

Chulalongkorn University Theses and Dissertations (Chula ETD)

เชื้อแบคทีเรียแกรมลบ Acinetobacter baumannii เป็นเชื้อฉวยโอกาส ที่ก่อให้เกิดการติดเชื้อในโรงพยาบาล และมีการดื้อต่อยาปฏิชีวนะหลายชนิด จากหลายกลไกการดื้อยา รวมถึงการใช้ efflux pump ขับยาออกนอกเซลล์ การศึกษาครั้งนี้ผู้วิจัยได้ทดสอบหาค่าความเข้มข้นต่ำสุดที่ยับยั้งการเจริญเติบโตของเชื้อ (MIC) ต่อยา amikacin ในเชื้อ A. baumannii ทั้งหมด 8 ตัวอย่าง ที่ได้จากสิ่งส่งตรวจของผู้ป่วย และเชื้อสายพันธุ์มาตรฐาน ATCC 19606 พบว่าเชื้อ A. baumannii 7 ตัวอย่างมีค่า MIC อยู่ในช่วง 0.25 – 4 µg/mL (ไวต่อยา) และเชื้ออีก 2 ตัวอย่างมีค่า MIC เท่ากับ 64 และมากกว่า 256 µg/mL (ดื้อต่อยา) เมื่อเหนี่ยวนำเชื้อด้วยยา amikacin โดยเพาะเลี้ยงเชื้อในสภาวะที่มียา amikacin ความเข้มข้นเพิ่มขึ้นเป็นสองเท่า ทุก 48 ชั่วโมง ติดต่อกันเป็นเวลาหลายวัน พบว่าเชื้อ A. baumannii หลังเหนี่ยวนำด้วยยา amikacin ทั้ง 8 ตัวอย่าง มีค่า MIC เพิ่มขึ้นอยู่ในช่วง 32 ถึงมากกว่า 256 µg/mL ผลการศึกษาการใช้สารยับยั้ง efflux pump ได้แก่ สาร carbonylcyanide m-chlorophenylhydrazone (CCCP) พบว่าเชื้อ A. baumannii หลังเหนี่ยวนำด้วยยา amikacin ทั้ง 8 ตัวอย่าง มีค่า MIC ต่อยา amikacin เมื่อมีสาร CCCP ลดลงมากกว่าหรือเท่ากับ 4 เท่าของค่า MIC ต่อยา amikacin ที่ไม่มีสาร …

The Clinicopathological Study And Associated Causes Of Pleural Effusions In Cats, Hsu Mon Hla

Chulalongkorn University Theses and Dissertations (Chula ETD)

Pleural effusion is an excessive and abnormal accumulation of fluid in the pleural space resulting from increased hydrostatic pressure, increased permeability of vascular capillary, and often leading to significant mortality. The objectives of this study were to investigate the clinicopathological parameters obtained by cytological and biochemical examination of pleural effusion and evaluate the correlations between the abnormality of these parameters and underlying causes of the pleural effusion in cats. This study was carried out in 127 cats with pleural effusion visiting the Small Animal Hospital, Faculty of Veterinary Science, Chulalongkorn University during the year 2020–2021. Pleural fluid samples were routinely …

Analysis Of Protein-Protein Interaction Network From Leukocyte Transcriptomic Profiles In Severe Covid -19 Patients, Pakorn Sagulkoo

Chulalongkorn University Theses and Dissertations (Chula ETD)

Coronavirus disease 2019 (COVID-19) still provides global public health issues although several vaccines and antiviral agents have been developed. Some patients experience severe conditions needed medical intensive care, and some are dead due to the failure of treatments. Therefore, identifying the key genes and underlying molecular mechanisms is necessary to discover precisely targeted drugs. Analysis of protein-protein interaction (PPI) networks provides invaluable information to find disease mechanisms and effective alternative drugs. Hence, PPI network analysis based on leukocyte transcriptomic profiles of severe COVID-19 collected from Gene Expression Omnibus (GEO) DataSets was proposed for this study. A network diffusion method called …

Mrna Vaccine Encoding Neoantigen For Cancer Immunotherapy Using Mouse Melanoma As A Model, Chutamath Sittplangkoon

Chulalongkorn University Theses and Dissertations (Chula ETD)

Cancer is the leading cause of death globally with an urgent need to find more effective approaches for prevention and treatment. mRNA vaccine is a promising vaccine platform over conventional vaccines in terms of safety, ease of large-scale production and effectiveness in inducing both cellular and humoral immune responses. Breakthrough in mRNA technology allowed novel strategies to improve mRNA stability and efficacy including nucleoside modifications. Modification of mRNA with N1-methylpseudouridine (m1Ψ) was reported to enhance protein expression and decrease innate immunogenicity due to low binding affinity to pattern recognition receptors, and low type I interferon (IFN-I) secretion. Therefore, this study …

Association Between Gene Expression And Promoter Methylation Of Cgb3, Gstp1 And Nop56 Genes And Their Potential In Biomarker Development - Associated Cervical Cancer., Palak Singh

Chulalongkorn University Theses and Dissertations (Chula ETD)

Binding of DNMT1-E7 complex can induce promoter methylation in tumor suppressor genes, such as CCNA1, CADM1 leading to their expression suppression and thus, cause cancer progression. This research establishes the association between methylation-related expression of CGB3 and NOP56 genes in cervical cell lines treated with 5-Azacytidine drug. Using MSP-PCR and qPCR, it was found that the methylation of CGB3 and NOP56 were significantly decreased as concentration of 5-Aza increased and this was inversely related with their expression. This demonstrated the association between gene methylation and the regulation of gene expression which were assessed by 5’-aza drug treatment. Of note, the …

Object Detection In Intelligent Billing System For Conveyor Belt Sushi Restaurant, Rangrak Maitriboriruks

Chulalongkorn University Theses and Dissertations (Chula ETD)

Organization must automate wherever and whenever they can, particularly during today’s global changes in daily lifestyles. Trends regarding the use of technology, especially AI has emerged as a key enabler for disruptive innovation. This thesis thus presents the application programming interface of object detector implemented with YOLOv4 and OpenCV for classifying the prices of sushi plates distinguished by colors. The object detector is part of the smart cross-platform mobile application to facilitate billing process for conveyor belt sushi business. The frontend is developed with Flutter to build single codebase for UIs. To handle the variants of image colors resulting from …

Health Risk Assessment Of Toxic Metals In Commonly Consumed Salad Leafy Vegetables In Bangkok, Zin Htoo Hlyan

Chulalongkorn University Theses and Dissertations (Chula ETD)

The objectives of this study were 1) to investigate concentrations of toxic metals including As, Cd, and Pb in the commonly consumed salad leafy vegetables which were sold in Bangkok, and 2) to assess potential human health risks of As, Cd, and Pb exposure via salad leafy vegetables consumption. A total of 120 samples were randomly collected from local fresh markets in Bangkok. The total concentrations of toxic metals were determined by an inductively coupled plasma mass spectroscopy (ICP-MS). Total concentrations of As, Cd and Pb in coral lettuce (CL), red coral (RC) and green oak (GO) for soil cultivation …

Global Analysis Of A Discrete Sirs Epidemic Model With Nonlinear Incidence Rate, Butsayapat Chaihao

Chulalongkorn University Theses and Dissertations (Chula ETD)

In this thesis, we prove some behaviors of a discrete SIRS epidemic model with a nonlinear incidence rate and a distributed time-delay. This model is constructed from the discretization of the corresponding continuous model by using a nonstandard finite difference method. The basic properties including the positivity and the boundedness of the solutions are established. We derive the existence of the disease-free equilibrium and the endemic equilibrium of the model. In addition, by applying Lyapunov function techniques, we prove that the disease-free equilibrium is globally attractive. Moreover, we give a sufficient condition for the permanence of the model. In order …

The Effect Of Xylitol And Erythritol In The Presence Of Sucrose On Single And Mixed-Species Biofilm Of Early Colonizer And Cariogenic Streptococci, Panadda Yuapibanrak

Chulalongkorn University Theses and Dissertations (Chula ETD)

Dental caries is caused by multiple factors. An essential factor is sucrose. Dental caries is the result of an imbalance between early colonizers and cariogenic bacteria in biofilms. Sugar alcohols, xylitol and erythritol, become more popular because they do not cause cavities and are also effective at preventing caries. In everyday life, however, sucrose consumption is unavoidable. Thus, the aim of this study was to investigate the effect of xylitol and erythritol in the presence of sucrose on single- and mixed-species biofilm of early colonizers and cariogenic bacteria. Bacteria were cultured in broth containing 1% sucrose compared to broth containing …

Identification Of Snp Barcode For Classify Carbapenem-Resistant Enterobacteriaceae (Cre) And Non-Cre Using Nanopore Sequencing, Nicha Sangpiromapichai

Chulalongkorn University Theses and Dissertations (Chula ETD)

Background: The continuous increase of carbapenem-resistant Enterobacteriaceae (CRE) prevalence has been a serious problem for public health worldwide. In the previous study, 25% of 3864 Klebsiella pneumoniae isolates were carbapenem-resistant. One of the most important risks of bacterial drug resistance is the improper use of antibiotics. The routine antimicrobial-susceptibility test requires overnight and has a turnaround time of approximately 2–3 days which delays the proper antibiotics. This study aims to develop a technique to classify CRE and non-CRE by SNP barcode. Method: Forty Klebsiella pneumoniae (KP) clinical isolates were collected from 2020–2021 at King Chulalongkorn Memorial Hospital. Twenty samples were …

A Pilot Study, Randomized Control Trial On Effect Of Oral Calcium Carbonate To Fecal Functional Levofloxacin Concentration In Healthy Volunteer Taking Oral Levofloxacin, Ophat Janphet

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objective: We conducted a pilot randomized control trial (RCT) to study whether oral calcium carbonate (CaCO3) can lower fecal levofloxacin (LVX) concentration and preserve gut microbiota diversity in healthy volunteers. Methods: The healthy volunteers received a 5-day course of once-daily 500 mg LVX oral tablet and were randomly assigned to treatment (6-day course of 1,000 mg CaCO3 oral tablet twice daily) and control group (no CaCO3). The primary outcome was fecal LVX concentration by MIC and high-performance liquid chromatography (HPLC) on day 2 and 5. The secondary outcomes were fecal microbiota diversity Shannon index (H) by 16s rDNA analysis, plasma …

Expression Of Vascular Endothelial Growth Factor Protein In Mrna-Transfected Human Periodontal Ligament Cells, Mana Naratippakorn

Chulalongkorn University Theses and Dissertations (Chula ETD)

The complete regeneration of periodontal tissues following current periodontal therapy remain challenging and unpredictable. Nucleoside-modified messenger RNA (mRNA) technology can be a promising novel platform in regenerative medicine. The aims of this study were to evaluate whether pseudouridine modified mRNA encoding vascular endothelial growth factor (VEGF) could induce VEGF production in human periodontal ligament cells (PDLCs) and this translated protein function by promoting in vivo blood vessel formation using chorioallantoic membrane (CAM) assay. Isolated PDLCs from healthy periodontal tissue were transfected with modified mRNA encoding VEGF (VEGF mRNA) complexed with a transfecting agent, Lipofectamine 2000 (L2000) and L2000 alone (control). …