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Probing The Assembly, Functional Relevance, And Dynamics Of Acra In Multi Drug Efflux In Escherichia Coli, Isoiza Ojo

Theses and Dissertations--Chemistry

Efflux pumps and low permeability of the outer membrane of gram-negative bacteria are major culprits in drug resistance. The most studied of these multidrug efflux pumps is the AcrAB-TolC, a complex of three proteins, the inner membrane transporter AcrB, the periplasmic adaptor AcrA and outer membrane factor TolC. This pump exports a wide range of molecules such as dyes, detergents, and antibiotics out of the bacteria system into the external environment. AcrB and TolC exist as obligate trimers, but the structure of AcrA during the assembly process is not well defined. My research aims to explore the functional and structural …

From Quandary Cases To Ordinary Life: New Opportunities To Connect Social Ethics And Health Care Ethics, Conor M. Kelly

Theology Faculty Research and Publications

In Christian bioethics, the call for a greater integration of social ethics and medical ethics is a popular refrain, yet lasting progress toward this goal has been elusive, in part due to the traditional emphasis on quandary cases in medical ethics. This article develops an alternative approach to moral discernment in health care, employing a theological interpretation of solidarity to promote greater social consciousness in ordinary health care decision making. This shifts the ethical analysis from abstract scenarios to everyday choices, elevating the moral significance of seemingly mundane concerns like antibiotic use and diet and exercise.

Application Of Data And Geospatial Analysis In Energy And Health Systems, Ali Faghani

Graduate Research Theses & Dissertations

Having sustainable energy and health systems are the main factors in the vision plan of every country. Both of these systems are correlated with a variety of frameworks, including social, physical, technological, political, and economic factors. Therefore, different types of analytics methods can be implemented to develop the required assessments for those who make plans since understanding the effect of such factors individually, also their interactions, and the overall effect is crucial. With this regard, applications of data analysis and geospatial techniques in both energy, and health systems have recently gained attention. The proposed research here deployed advanced data analytics …

Characterization Of Potential Anti-Infective Agents By Targeting Burkholderia Pseudomallei And Escherichia Coli Ispf, Dakota Lane Grote

Graduate Research Theses & Dissertations

With the likelihood of contracting an antibiotic resistant infection increasing, the need for novel anti-infective agents is critical. The development of novel anti-infective agents benefits from the identification of new target pathways necessary for the survival of the pathogen. One such target is the methylerythritol phosphate (MEP) pathway, responsible for the synthesis of the isoprenoid precursors isopentyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP) in bacteria and other pathogenic organisms. The MEP pathway is an attractive target because it is specific to a wide range of pathogenic organisms, while humans have a separate pathway, the mevalonate (MVA) pathway, for the biosynthesis …

A Time Series Analysis Approach To Forecasting Covid-19 Cases And Deaths: An Analysis Of Covid-19 Data In Colombia, Andrea Jackson-Sagredo

Graduate Research Theses & Dissertations

The novel Coronavirus, known as COVID-19 is a highly contagious and transmissible infectious disease that has taken a toll throughout the entire world for over a year. The inner workings and long term effects of COVID-19 continue to be misunderstood. While COVID-19 has impacted all countries tremendously, Latin American countries and specifically Colombia have been impacted significantly by the virus. This thesis investigates the potential to forecast COVID-19 cases and deaths using Time Series Analysis methods and models for the South American country of Colombia. Time series analysis on Colombian COVID-19 data begins with data processing on a data set …

Development Of Novel Polymeric Materials For Their Application In Monitoring And Remediation Of Environmental Pollutants, Rishabh Shah

Theses and Dissertations--Chemical and Materials Engineering

Remediation of environmental pollutants from water is one of the major challenges in the 21st century. Utilizing novel polymeric materials to accomplish this challenge has garnered a lot of interest in recent times. Flexibility in synthesizing as well as functionalizing makes them attractive for their application in pollutant remediation. This work is based on development and characterization of novel crosslinked polymeric as well as linear polymeric materials from biphenyl-based monomers, biphenyl based crosslinker and a temperature responsive monomer (Nisopropylacrylamide (NIPAAm)) for their application in remediation of toxic pollutants such as polychlorinated biphenyls (PCBs), polyaromatic hydrocarbons (PAHs) and iron oxide nanoparticle …

Cumulative Risk, Parenting, And Adolescents’ Problem Behaviors In Ghana, Braima Salaam

Graduate Research Theses & Dissertations

The accumulation of multiple risk factors predisposes adolescents to problem behaviors such as anxiety, depression, and aggression, whereas parental warmth and behavioral control are protective by lowering adolescents’ experiences of these problem behaviors. Research in predominantly Western, industrialized, and rich contexts has shown that in stressful family environments, cumulative co-occurring risks and parenting factors act together to influence adolescents’ problem behaviors. In particular, studies have shown that parental warmth and behavioral control mediate and moderate the effect of cumulative risk to predict adolescents’ problem behaviors. The present study examines the effect of cumulative risk, and mothers’ and fathers’ warmth and …

Vbms 403: Integrated Principles And Prevention Of Livestock Diseases, Christina L. Topliff

UNL Faculty Course Portfolios

This portfolio documents the teaching objectives, strategies, assessments, and changes implemented for VBMS 403: Integrated Principles and Prevention of Livestock Diseases, an Achievement-Centered Education (ACE) 10 Capstone course taught during the Spring Semester through the School of Veterinary Medicine and Biomedical Sciences at the University of Nebraska-Lincoln. Teaching methods and course activities included traditional lecturing with quizzes and examinations, in-class discussions, short reading assignments, and the generation of a scholarly term paper demonstrating broad knowledge, technical proficiency, information collection, synthesis, interpretation, and presentation. This portfolio documents the qualitative and quantitative methods used to assess the course learning objectives (goals). My …

Global Regulation Of Germline Genome Editing: Ethical Considerations And Application Of International Human Rights Law, Scott J. Schweikart

Loyola of Los Angeles International and Comparative Law Review

No abstract provided.

The Primary Care Provider’S Role In Screening And Intervening In The Human Trafficking Of Adolescents: A Systematic Literature Review, Brittany Schmid

All Graduate Theses, Dissertations, and Other Capstone Projects

Human trafficking (HT) is a growing concern within the United States (U.S.) and internationally. With the alarming number of HT victims presenting for medical care, primary care providers (PCPs) are in a unique and privileged position to encounter HT victims within the healthcare system. Adolescents, in particular, are vulnerable to HT because of minimal life experiences, inadequate social support, and limited coping mechanisms (Ertl et al., 2020). Failure to recognize and intervene in cases involving HT victims who are minors is detrimental to the individual’s health, wellbeing, and safety. With the assistance of education and training, community resources, guideline adoption, …

Understanding The Implications Of Hpv Infection: Does Parental Education Impact Hpv Vaccination Completion Rates?, Heather A. Pederson

All Graduate Theses, Dissertations, and Other Capstone Projects

Up to 80% of United States (U.S.) citizens will contract the human papillomavirus (HPV) at some point in their lives. HPV has the ability to cause multiple types of cancer, including cervical, vulvar, vaginal, penile, anal, and oropharyngeal. The most recent HPV vaccine is effective against nine strains of the virus, which account for most cases of cancer and genital warts caused by HPV. Parents are a key component in reaching Healthy People 2030’s goal to have 80% of all adolescents in the U.S. completely vaccinated against HPV. Objective: The purpose of this systematic review was to determine if …

An Evaluation Of The Performance Of Proc Arima's Identify Statement: A Data-Driven Approach Using Covid-19 Cases And Deaths In Florida, Fahmida Akter Shahela

Electronic Theses and Dissertations, 2020-2023

Understanding data on novel coronavirus (COVID-19) pandemic, and modeling such data over time are crucial for decision making at managing, fighting, and controlling the spread of this emerging disease. This thesis work looks at some aspects of exploratory analysis and modeling of COVID-19 data obtained from the Florida Department of Health (FDOH). In particular, the present work is devoted to data collection, preparation, description, and modeling of COVID-19 cases and deaths reported by FDOH between March 12, 2020, and April 30, 2021. For modeling data on both cases and deaths, this thesis utilized an autoregressive integrated moving average (ARIMA) times …

Witch Pamphlets, Tsea M. Francisconi

Graduate Student Theses, Dissertations, & Professional Papers

The witch hysteria that overtook Christian Europe during the Early Modern era inspired a mass paranoia over the conspiratorial belief that the Abrahamic religion’s personification of the world’s evils, also known as Satan, the Devil, demons, or Lucifer interchangeably, was attempting to rise up and cause harm to Christian communities during this time period. It was believed that in order to achieve this goal the Christian version of the Devil had been recruiting humans within Christian communities and turning these chosen humans into witches by granting them the ability to wield magical powers to spread their destruction, murder, and terror …

Characterizing A Novel Cocksackievirus B3 Protease Mutant And Its Response To Polyamine Depletion, Bridget Hulsebosch

Master's Theses

Enteroviruses, including Coxsackievirus B3 (CVB3), are pervasive pathogens that cause significant disease, including cardiomyopathies. Unfortunately, no treatments or vaccines are available for infected individuals. We identified the host polyamine pathway as a potential drug target, as inhibiting polyamine biosynthesis significantly reduces enterovirus replication in vitro and in vivo. Here, we show that CVB3 is sensitive to polyamine depletion through the polyamine analog diethylnorspermidine (DENSpm) which enhances polyamine catabolism through induction of polyamine acetylation. We demonstrate that CVB3 acquires resistance to DENSpm via mutation of the 2A protease, which enhances proteolytic activity in the presence of DENSpm. Resistance to DENSpm occurred …

The Impact Of The Th17:Treg Axis On The Iga-Biome Across The Glycemic Spectrum, Heather T Essigmann, Kristi L Hoffman, Joseph F Petrosino, Goo Jun, David Aguilar, Craig L Hanis, Herbert L Dupont, Eric L Brown

Faculty, Staff and Student Publications

Secretory IgA (SIgA) is released into mucosal surfaces where its function extends beyond that of host defense to include the shaping of resident microbial communities by mediating exclusion/inclusion of respective microbes and regulating bacterial gene expression. In this capacity, SIgA acts as the fulcrum on which host immunity and the health of the microbiota are balanced. We recently completed an analysis of the gut and salivary IgA-Biomes (16S rDNA sequencing of SIgA-coated/uncoated bacteria) in Mexican-American adults that identified IgA-Biome differences across the glycemic spectrum. As Th17:Treg ratio imbalances are associated with gut microbiome dysbiosis and chronic inflammatory conditions such as …

N95 Respirator Reuse, Decontamination Methods, And Microbial Burden: A Randomized Controlled Trial, Zi Yang Jiang, Zhen Huang, Isaac Schmale, Eric L Brown, Michael C Lorenz, Scott J Patlovich, Karan Goswami, Hannah B Wilson, Jumah Ahmad, Ronda Alexander, William Bryan, Luke Burke, Martin J Citardi, Jose Elias, Tang Ho, Jack Jacob, Garren Low, Pedro Miramón, Aniruddha U Patki, William C Yao, Amber U Luong

Faculty, Staff and Student Publications

PURPOSE: to evaluate the effectiveness and ease of N95 respirator decontamination methods in a clinic setting and to identify the extent of microbial colonization on respirators associated with reuse.

METHODS: In a prospective fashion, N95 respirators (n = 15) were randomized to a decontamination process (time, dry heat, or ultraviolet C light [UVC]) in outpatient clinics. Each respirator was re-used up to 5 separate clinic sessions. Swabs on each respirator for SARS-CoV-2, bacteria, and fungi were obtained before clinic, after clinic and post-treatment. Mask integrity was checked after each treatment (n = 68). Statistical analyses were performed to determine factors …

Conservation And Conversation In The Anthropocene, Brittany Alyse Garner

Graduate Student Theses, Dissertations, & Professional Papers

The driving questions of this dissertation consider both the scientific and communicative understanding of conservation and extinction in the Anthropocene. Overall, this dissertation portfolio explores this topic via genetic and genomic data, international policy, big data analytics, human values, and the performing arts. Chapter 1 analyzes the use of genetics and genomics in conservation, finding multiple case studies within and outside of peer-reviewed journals. Chapters 2 and 3 quantify and describe the use of genetic principles and tools in a global species extinction risk assessment, the IUCN Red List, and finds low overall use but high potential for genetics to …

Discourse Of Renewal: A Qualitative Analysis Of The University Of Montana’S Covid-19 Crisis Communication, Haley Renae Gabel

Graduate Student Theses, Dissertations, & Professional Papers

The COVID-19 pandemic has proven to be a public health crisis that has affected lives and impacted organizations across the world. The pandemic has interrupted student learning and threatened universities nationwide. Crises are trying times for organizations, and when faced with a crisis, an organization has an obligation to keep its members informed; therefore, it must carefully choose how to respond and communicate about the crisis. This study uses discourse of renewal theory as a lens to analyze the University of Montana’s communication about COVID-19, studying 37 pages of university emails and documents on the university website. Results indicate that …

Impact Of Out-Of-Pocket Fees On Under-5 Infant Mortality In Cameroon, Amelie Olga Mafotsing Fopoussi

Walden Dissertations and Doctoral Studies

Health facilities in Cameroon charge fees for treatment and services, which can affect health outcomes for those unable to pay for services. Some studies have shown that the removal of user fees had a positive impact on child health outcomes in selected African countries. The aim of this quantitative, cross-sectional study was to understand whether there is a link between out-of-pocket expenses and under-5 mortality in Cameron. The study was guided by the social ecological framework. Secondary data from the 2011 Cameroon Demographic Health Survey were used in the study. Chi-square, binary logistic regression, and multicollinearity analyses were used to …

Examining Terrorism Attacks And Regional Preparedness In The United States, Trebor Curtis Yocum

Walden Dissertations and Doctoral Studies

After the 9/11 terrorist attacks, the U.S. government renewed efforts to prepare for future attacks. Despite research on federal and state government preparedness, there was a lack of scholarship on trends in terrorist attacks at the local level. The purpose of this quantitative descriptive study was to examine trends in terrorism attacks in the United States between 2001 and 2018 and determine whether significance exists between characteristics of the terrorism incidents (weapon used, target type) and region. The conceptual framework included Grundmann’s risk management and Tomuzia et al.’s risk assessment scenario models. Answering the research questions entailed examining trends in …

Demographic And Socioeconomic Determinants Of Lassa Fever In Edo State, Nigeria, Faith Ogagaroghene Ireye

Walden Dissertations and Doctoral Studies

Lassa fever is a viral hemorrhagic fever caused by the Lassa virus and associated with significant morbidity and mortality. Yearly outbreaks of the disease occur in Edo State, Nigeria. The purpose of this study was to examine the demographic and socioeconomic factors that can influence transmission of the disease. The Commission on Social Determinants of Health conceptual framework was used in this research study. This framework describes the relationship between structural and intermediary factors and how they determine health outcomes. To answer the research question of how demographic and socioeconomic factors influence Lassa fever transmission in Edo State, a cross-sectional …

Staff Education Program For Chlamydia And Gonorrhea Self-Collected Vaginal Swabbing, Cheryl Marie Tugman

Walden Dissertations and Doctoral Studies

Girls and women carry the burden of negative consequences of untreated sexually transmitted diseases (STDs), such as chlamydia and gonorrhea. Screening for chlamydia and gonorrhea by increasing self-collected vaginal swabbing (SCVS) can improve access of care and prevent delayed treatment. Nurses may support their patients if they receive training in how to teach screening for sexual self-healthcare. The purpose of this project was to develop and conduct a staff education program in an urban primary care clinic. The project addressed whether the education program would increase nurses’ knowledge of SCVS. The goal is to ultimately increase female patients’ access to …

The Role Of The Public During The Decision-Making Process In Preparation For And In Response To A Pandemic, Ludmila M. Flores

Walden Dissertations and Doctoral Studies

AbstractMany advances have been made in technology and medicine; however, humanity remains vulnerable to existing, emerging, and re-emerging infectious diseases that have a profound negative impact on our society. This study investigated how individuals balance socioeconomics, demographics, and religious views with their actual behavior in response to public health guidelines during an epidemic/pandemic. The participants' perceptions and experiences of a practical problem, how the public was asked to respond, and how the public ultimately responded to public health guidelines were explored. The theoretical model for the study, the polarity of democracy model, has been explored as a possible decision-making tool …

Sociodemographic Factors Associated With Childhood Vaccination Status In Sokoto State, Nigeria, Dauda Milgwe Madubu

Walden Dissertations and Doctoral Studies

Immunization remains one of the most successful and cost-effective public health interventions worldwide. Despite the critical role of vaccines in improving childhood and maternal health, Sokoto state recorded the lowest childhood vaccination completion rate among the 37 states in Nigeria with only 5% of children aged 12-23 months having had full childhood vaccination based on data from the 2018 demographic and health survey. The factors associated with the state’s low childhood vaccination status have not been explored. The study examined the relationship between the sociodemographic factors including parents’ socioeconomic and ethnoreligious factors, place of residence, and children’s biological characteristics and …

Associations Between The Use Of Bed Nets And Malaria Infection On The School Performance Of Juveniles In Ghana, Ans Bumbokuri

Walden Dissertations and Doctoral Studies

Millions of malaria cases are reported yearly in sub-Saharan Africa, events which adversely influence children’s school performance. The purpose of this quantitative, cross-sectional study was to investigate the effectiveness of major malaria control policies in reducing malaria cases as a means of improving academic performance among school children in the greater Accra region of Ghana. A secondary data set was obtained from the 2014-2016 data from the Ghana Malaria Indicator Survey (GMIS). Findings indicated that bed net use and malarial episodes in children of participant recorded a p-value of 0.43 which is a significant association between the variables. The likelihood …

Surgical Site Infection Rates After Implementation Of The Surgical Care Improvement Project Initiative, Victor Lao De Guzman Jr

Walden Dissertations and Doctoral Studies

Surgical site infections (SSIs) reflect a serious complication in modern healthcare and are a substantial burden to healthcare systems and service payers worldwide in terms of patient morbidity, mortality, and additional costs. The Surgical Care Improvement Project (SCIP), introduced in 2006, was developed by the Centers for Medicare and Medicaid Services to reduce SSI rates by 25%. However, SCIP was retired in 2015. Given the considerable financial burden of SSIs and because SSIs may be prevented using evidence-based measures, it was worth revisiting and re-evaluating the quality improvement efforts brought about by the success of the evidence-based SCIP initiative. This …

The Experience Of Women With Opioid Use Disorder Accessing Methadone Treatment, Lizette Cristine Keenan

Walden Dissertations and Doctoral Studies

The number of women experiencing opioid use disorder (OUD) in Canada has increased exponentially. In Canada, healthcare is socialized and free for all citizens and often medications like methadone are free as well, yet few individuals with OUD access treatment services. The purpose of this study was to describe the lived experiences of Canadian women with OUD who were receiving methadone treatment. Interpretive phenomenology was used to investigate the treatment experiences of seven women with OUD. The conceptual framework of self-care of chronic illness was used examine this phenomenon. Data was analyzed using a seven step process of interpretive phenomenological …

Determinants Of Substance Abuse Among Users With Mental Health Issues In Ogun State, Nigeria, Michael Olusegun Adejimi

Walden Dissertations and Doctoral Studies

In Nigeria, about 14.3 million people use drugs without physician prescription and approximately 30% of Nigerians suffer from mental illness, some of which is due to substance abuse. This study examined the associations between (a) socio-demographic factors and substance abuse among users with mental health issues in Ogun State Nigeria, (b) clinical variables and substance abuse among users with mental health issues, (c) negative life events and substance abuse among users with mental health issues, and (d) social supports and substance abuse among users with mental health issues. This study theoretical framework was based on the definition of mental health …

Reducing Infection Rates Through Handwashing Education, Maureen N. Osegbe

Walden Dissertations and Doctoral Studies

AbstractHealth care-associated infections (HCAIs) affect millions of people worldwide, causing morbidity and mortality among hospitalized patients. Hand hygiene is the single most effective way to prevent HCAIs. This project addressed a gap in practice regarding nurses’ knowledge on the effective use of hand hygiene in a medical/surgical unit in a hospital located in northeastern Maryland. This project addressed the question of whether an educational program on handwashing for nurses reduced HCAI rates. A literature review was conducted to support the development of the educational program; relevant sources included peer-reviewed journal articles, systematic reviews, and guidelines that supported the practice-focused question, …

Antibiotic Stewardship: Decreasing Antibiotic Use For Viral Upper Respiratory Infections, Merkessie Taresa Redix

Walden Dissertations and Doctoral Studies

AbstractOverprescribing antibiotics for viral upper respiratory infections is a common practice in the United States. This overprescribing of antibiotics can cause several health issues and increase health care costs. Despite the current guidelines recommended by the Centers for Disease Control and Prevention (CDC) the overprescribing of antibiotics by providers remains an issue. The purpose of this project was to educate providers on antibiotic stewardship for viral upper respiratory infections. The practice question was used to explore whether an educational program using concepts from Lewin’s change theory and Kolb’s learning theory would increase provider’s knowledge about the current evidence- based prescriptive …