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Thinking Outside Malaria: A Rare Case Of Disseminated Cysticercosis With Cardiopulmonary Involvement From Urban Tanzania, Frederick R. Lyimo, Ahmed Jusabani, Hilda Makungu, Maria Mtolera, Salim Surani

Imaging & Diagnostic Radiology, East Africa

Dissemination of the cysticerci throughout the body with cardiopulmonary involvement represents a very rare occurrence and an uncommon form of cysticercosis manifestation. We report a rare case of a 48-yearold African male from urban Tanzania who was, at first, referred to our radiology department for a coronary computed tomography angiography (CCTA), but incidentally on further evaluation of the patient revealed a history of recurrent convulsions, loss of consciousness, a single episode of temporary loss of vision and recent skin nodules. The value of a full clinical and radiological evaluation of the patient presenting with adult-onset seizures cannot be overemphasized for …

Activating Dormant Strongyloidiasis Secondary To Covid-19 Treatment, Holman Li, Fahad Alkaaba, Farrah Ibrahim

UAB Huntsville Research Day - All Presentations

2021 clinical vignette poster

Coronavirus Disease (Covid-19) In A Solid Organ Transplant Recipient, Mariam Riad, Dhivya Velu, Qurrat-Ul-Ain Aziz, Ali Hassoun

UAB Huntsville Research Day - All Presentations

2021 clinical vignette poster

Chronic Salmonella Prosthetic Joint Infection Complicated By Atrial Fibrillation, James A. Stewart, Ali Ahmed, Ali Hassoun

UAB Huntsville Research Day - All Presentations

2021 clinical vignette poster

Covid-19 Reinfection With A Rare Variant Of Sars-Cov-2: The First Reported Case Of P.2 Variant In Alabama, Gabrielle Wilhelm, Ali Hassoun

UAB Huntsville Research Day - All Presentations

2021 clinical vignette poster

Challenges In The Diagnosis Of Covid-19 Patients, Miriam Riad, Elizabeth Thottacherry, Ali Hassoun

UAB Huntsville Research Day - All Presentations

2021 research poster

Global Distribution, Host Range And Prevalence Of Trypanosoma Vivax: A Systematic Review And Meta-Analysis, Eyerusalem Fetene, Samson Leta, Fikru Regassa, Philippe Büscher

Harold W. Manter Laboratory of Parasitology: Library Materials

Background

Trypanosomosis caused by Trypanosoma vivax is one of the diseases threatening the health and productivity of livestock in Africa and Latin America. Trypanosoma vivax is mainly transmitted by tsetse fies; however, the parasite has also acquired the ability to be transmitted mechanically by hematophagous dipterans. Understanding its distribution, host range and prevalence is a key step in local and global efforts to control the disease.

Methods

The study was conducted according to the methodological recommendations of the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) checklist. A systematic literature search was conducted on three search engines, namely PubMed, …

Human Body-Fluid Proteome: Quantitative Profiling And Computational Prediction, Lan Huang, Dan Shao, Yan Wang, Xueteng Cui, Yufei Li, Qian Chen, Juan Cui

School of Computing: Faculty Publications

Empowered by the advancement of high-throughput bio technologies, recent research on body-fluid proteomes has led to the discoveries of numerous novel disease biomarkers and therapeutic drugs. In the meantime, a tremendous progress in disclosing the body-f luid proteomes was made, resulting in a collection of over 15 000 different proteins detected in major human body fluids. However, common challenges remain with current proteomics technologies about how to effectively handle the large variety of protein modifications in those fluids. To this end, computational effort utilizing statistical and machine-learning approaches has shown early successes in identifying biomarker proteins in specific human diseases. …

Effect Of High-Pressure Processing On The Microbial Load And Functionality Of Sugar-Cookie Dough, Luis Sabillón, Jayne E. Stratton, Devin Rose, Kent M. Eskridge, Andreia Bianchini

Department of Food Science and Technology: Faculty Publications

Background and objectives: Refrigerated dough products have the potential to be a safety hazard to consumers because they could be consumed raw or undercooked. The objectives of this study were designed to evaluate the microbial and functionality changes in high pressured sugar-cookie dough as a function of a_w (0.80–0.87), pressure level (100–600 MPa), and holding time (1–6 min).

Findings: Endogenous microbial populations were marginally reduced (0.2–0.5 log CFU/g) by pressure treatments. However, treating the dough at 600 MPa for 6 min significantly reduced counts of inoculated Escherichia coli by as much as 2.0 log CFU/g. Increasing the a_{w …}

Bovine Colostrum And Its Potential For Human Health And Nutrition, Aysenur Arslan, Merve Kaplan, Hatice Duman, Ayse Bayraktar, Melih Ertürk, Bethany M. Henrick, Steven A. Frese, Sercan Karav

Department of Food Science and Technology: Faculty Publications

Colostrum is the first milk produced post-partum by mammals and is compositionally distinct from mature milk. Bovine colostrum has a long history of consumption by humans, and there have been a number of studies investigating its potential for applications in human nutrition and health. Extensive characterization of the constituent fractions has identified a wealth of potentially bioactive molecules, their potential for shaping neonatal development, and the potential for their application beyond the neonatal period. Proteins, fats, glycans, minerals, and vitamins are abundant in colostrum, and advances in dairy processing technologies have enabled the advancement of bovine colostrum from relative limitations …

Interspecies Variation In Hominid Gut Microbiota Controls Host Gene Regulation, Amanda L. Muehlbauer, Allison L. Richards, Adnan Alazizi, Michael B. Burns, Andres Gomez, Jonathan B. Clayton, Klara Petrzelkova, Camilla Cascardo, Justyna Resztak, Xiaoquan Wen, Roger Pique-Regi, Francesca Luca, Ran Blekhman

Department of Food Science and Technology: Faculty Publications

The gut microbiome exhibits extreme compositional variation between hominid hosts. However, it is unclear how this variation impacts host physiology across species and whether this effect can be mediated through microbial regulation of host gene expression in interacting epithelial cells. Here, we characterize the transcriptional response of human colonic epithelial cells in vitro to live microbial communities extracted from humans, chimpanzees, gorillas, and orangutans. We find that most host genes exhibit a conserved response, whereby they respond similarly to the four hominid microbiomes. However, hundreds of host genes exhibit a divergent response, whereby they respond only to microbiomes from specific …

Dietary Fiber Intake, The Gut Microbiome, And Chronic Systemic Inflammation In A Cohort Of Adult Men, Wenjie Ma, Long H. Nguyen, Mingyang Song, Dong D. Wang, Eric A. Franzosa, Yin Cao, Amit Joshi, David A. Drew, Raaj Mehta, Kerry L. Ivey, Lisa L. Strate, Edward L. Giovannucci, Jacques Izard, Wendy Garrett, Eric B. Rimm, Curtis Huttenhower, Andrew T. Chan

Department of Food Science and Technology: Faculty Publications

Background: A higher intake of dietary fiber is associated with a decreased risk of chronic inflammatory diseases such as cardiovascular disease and inflammatory bowel disease. This may function in part due to abrogation of chronic systemic inflammation induced by factors such as dysbiotic gut communities. Data regarding the detailed influences of long-term and recent intake of differing dietary fiber sources on the human gut microbiome are lacking.

Methods: In a cohort of 307 generally healthy men, we examined gut microbiomes, profiled by shotgun metagenomic and metatranscriptomic sequencing, and long-term and recent dietary fiber intake in relation to plasma …

Integration Of The Metabolome And Transcriptome Reveals The Metabolites And Genes Related To Nutritional And Medicinal Value In Coriandrum Sativum, Wu Tong, Feng Shu-Yan, Yang Qi-Hang, Preetida J. Bhetariya, Gong Ke, Cui Chun-Lin, Song Jie, Ping Xiao-Rui, Pei Qiao-Ying, Yu Tong, Song Xiao-Ming

Department of Food Science and Technology: Faculty Publications

Coriandrum sativum (Coriander) or Chinese parsley is a culinary herb with multiple medicinal effects, which is widely used in cooking and traditional medicine. It is enriched with essential oils and anti-oxidant compounds with unknown significance. To explore the untapped reservoir of Coriander, we studied the transcriptome and metabolic profiles from three developmental stages. Here, we identified 10 tyrosine metabolic pathway-related genes (TMPRGs), six porphyrins and chlorophyll metabolic pathway-related genes (PCMPRGs), and five Vitamin E metabolic pathway-related genes (VEMPRGs). These genes were associated with the early development of Coriander. Our analysis suggests that these pathways …

High Prevalence Of Pre-Existing Serological Cross-Reactivity Against Severe Acute Respiratory Syndrome Coronavirus-2 (Sars- Cov-2) In Sub-Saharan Africa, For Yue Tso, Salum Lidenge, Phoebe B. Peña, Ashley A. Clegg, John R. Ngowi, Julius Mwaiselage, Owen Ngalamika, Peter Julius, John T. West, Charles Wood

Nebraska Center for Virology: Faculty Publications

Objective: Significant morbidity and mortality have occurred in the USA, Europe, and Asia due to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), whereas the numbers of infections and deaths in sub-Saharan Africa (SSA) have remained comparatively low. It has been hypothesized that exposure of the population in SSA to other coronaviruses prior to the COVID-19 pandemic resulted in some degree of cross-protection against SARS-CoV-2 infection and pathogenesis. We evaluated this hypothesis by comparing SARS-CoV-2 cross-reactive antibodies in pre-pandemic plasma samples collected from SSA and the USA.

Method: Pre-COVID-19 pandemic plasma samples from SSA and the USA were collected and tested by …

The 20th Anniversary Meeting Of The Rocky Mountain Virology Association, Joel Rovnak, Laura A. St Clair, Carley Mcalister, Chinemerem P. Ogbu, Jessica Smolenske, Randall J. Cohrs, Rushika Perera

Nebraska Center for Virology: Faculty Publications

Due to the COVID-19 pandemic and multiple devastating forest fires, the 2020 meeting of the Rocky Mountain Virology Association was held virtually. The three-day gathering featured talks describing recent advances in virology and prion research. The keynote presentation described the measles virus paradox of immune suppression and life-long immunity. Special invited speakers presented information concerning visualizing antiviral effector cell biology in mucosal tissues, uncovering the T-cell tropism of Epstein-Barr virus type 2, a history and current survey of coronavirus spike proteins, a summary of Zika virus vaccination and immunity, the innate immune response to flavivirus infections, a discussion concerning prion …

Chlorovirus Pbcv-1 Multidomain Protein A111/114r Has Three Glycosyltransferase Functions Involved In The Synthesis Of Atypical N-Glycans, Eric Noel, Anna Notaro, Immacolata Speciale, Garry A. Duncan, Cristina De Castro, James L. Van Etten

Nebraska Center for Virology: Faculty Publications

The structures of the four N-linked glycans from the prototype chlorovirus PBCV-1 major capsid protein do not resemble any other glycans in the three domains of life. All known chloroviruses and antigenic variants (or mutants) share a unique conserved central glycan core consisting of five sugars, except for antigenic mutant virus P1L6, which has four of the five sugars. A combination of ge- netic and structural analyses indicates that the protein coded by PBCV-1 gene a111/114r, conserved in all chloroviruses, is a glycosyltransferase with three putative domains of approximately 300 amino acids each. Here, in addition to in silico sequence …

Strategies Targeting Hemagglutinin As A Universal Influenza Vaccine, Brianna L. Bullard, Eric A. Weaver

Nebraska Center for Virology: Faculty Publications

Influenza virus has significant viral diversity, both through antigenic drift and shift, which makes development of a vaccine challenging. Current influenza vaccines are updated yearly to in- clude strains predicted to circulate in the upcoming influenza season, however this can lead to a mismatch which reduces vaccine efficacy. Several strategies targeting the most abundant and im- munogenic surface protein of influenza, the hemagglutinin (HA) protein, have been explored. These strategies include stalk-directed, consensus-based, and computationally derived HA immunogens. In this review, we explore vaccine strategies which utilize novel antigen design of the HA protein to improve cross-reactive immunity for development …

Zika Virus Infection Induces Endoplasmic Reticulum Stress And Apoptosis In Placental Trophoblasts, Philma Glora Muthuraj, Prakask K. Sahoo, Madison Kraus, Taylor Bruett, Arun S. Annamalai, Aryamav Pattnaik, Asit K. Pattnaik, Siddappa N. Byrareddy, Sathish Kumar Natarajan

Nebraska Center for Virology: Faculty Publications

Zika virus (ZIKV) infection to a pregnant woman can be vertically transmitted to the fetus via the placenta leading to Congenital Zika syndrome. This is characterized by microcephaly, retinal defects, and intrauterine growth retardation. ZIKV induces placental trophoblast apoptosis leading to severe abnormalities in the growth and development of the fetus. However, the molecular mechanism behind ZIKV-induced apoptosis in placental trophoblasts remains unclear. We hypothesize that ZIKV infection induces endoplasmic reticulum (ER) stress in the trophoblasts, and sustained ER stress results in apoptosis. HTR-8 (HTR-8/SVneo), a human normal immortalized trophoblast cell and human choriocarcinoma-derived cell lines (JEG-3 and JAR) were …

A Study On The Pathological Effects Of Trypanorhyncha Cestodes In Dusky Groupers Epinephelus Marginatus From The Canary Islands, Carolina De Sales-Ribeiro, Miguel A. Rivero, Antonio Fernández, Natalia García-Álvarez, Jorge Francisco González, Oscar Quesada-Canales, María José Caballero

Harold W. Manter Laboratory of Parasitology: Library Materials

Trypanorhyncha are cestodes commonly infecting marine fish. Numerous studies have detailed the biology of Trypanorhyncha species, but information on the pathological changes produced by these parasites is limited. Dusky groupers are keystone species necessary for the preservation of several marine ecosystems. Considering their vulnerable state of conservation and the efforts being made to culture them, identification of the effects caused by Trypanorhyncha is vital. Here, we aimed to determine the prevalence and pathological changes produced by Trypanorhyncha in dusky groupers from the Canary Islands. The prevalence of trypanorhynch plerocerci was 96%. Grossly, in the abdominal cavity, there were numerous larvae-filled …

Of Pigs And Men: The Best-Laid Plans For Prevention And Control Of Swine Fevers, Jishu Shi, Lihua Wang, David Scott Mcvey

School of Veterinary and Biomedical Sciences: Faculty Publications

John Steinbeck drew the title of his novel “Of Mice and Men” from a line in a Robert Burns poem “To a mouse”: “The best-laid plans of mice and men/Go often awry.” Unlike John Steinbeck who used the title to mirror the characters who were struggling during the Great Depression to the mouse whose nest was accidentally destroyed by the poet (Burns 1785), we chose this line to emphasize that the best-laid plan can go wrong in infectious disease control and prevention. Here, we will discuss the contributing factors behind the global successes and failures in the prevention and control …

Special Issue: Shiga Toxin-Producing Escherichia Coli, Rodney A. Moxley

School of Veterinary and Biomedical Sciences: Faculty Publications

Globally, Shiga toxin-producing Escherichia coli (STEC) is an important cause of diarrheal disease, most notably hemorrhagic colitis, and post-diarrheal sequela, such as hemolytic-uremic syndrome (HUS) [1]. Cattle are a major reservoir of STEC, with approx- imately half of the cases in humans attributable to foodborne exposure [2]. Prevention of human illness has mainly been through food safety measures [2]. Despite extensive research, no other generally accepted and effective preventive measures or therapies for STEC infections in human patients are available [3]. Many questions remain about STEC virulence factors, pathogenesis, detection, and other aspects that necessitate a continua- tion of basic …

Inhibition Of Zika Virus Replication By G-Quadruplex-Binding Ligands, Prativa Majee, Aryamav Pattnaik, Bikash R. Sahoo, Uma Shankar, Asit K. Pattnaik, Amit Kumar, Debasis Nayak

School of Veterinary and Biomedical Sciences: Faculty Publications

Zika virus (ZIKV), a mosquito-transmitted Flavivirus, emerged in the last decade causing serious diseases and affecting human health globally. Currently, no licensed vaccines or antivirals are available to combat ZIKV, although several vaccine candidates are in the pipeline. In recent years, the presence of non-canon- ical G-quadruplex (GQ) secondary structures in viral genomes has ignited significant attention as potential targets for anti- viral strategy. In this study, we identified several novel conserved potential GQ structures by analyzing published ZIKV genome sequences using an in-house algorithm. Bio- physical and biochemical analysis of the RNA sequences con- taining these potential GQ sequences …

An Analysis Of Covid-19 Vaccine Allergic Reactions, Robert Schumaker, Michael Veronin, Trevor Rohm, Rohit Dixit, Shadi Aljawarneh, Juan Lara

Journal of International Technology and Information Management

From our study, all three covid-19 vaccines have a similar proportion of adverse reaction reports in which the patient had a history of allergies. However, the proportion of life-threatening outcomes were lower for those with the Janssen vaccine (0.62% hospitalization rate for Janssen versus 2.59% for Pfizer and 0.60% death for Janssen versus 5.15% for Moderna). In terms of specific allergies, patients with *cillin or sulfa allergies had the most adverse reactions to covid-19 vaccines, however, Janssen again had the lowest percentage of reported deaths (1.39% for *cillin-related allergy deaths for Janssen versus 6.10% for Pfizer). In terms of patient …

Single-Dose Oral Challenges To Validate Eliciting Doses In Children With Cow’S Milk Allergy, Paul J. Turner, Yvonne M. D'Art, Bettina Duca, Sophia A. Chastell, Guadalupe Marco-Martin, Rosialzira N. Vera-Berrios, Olaya Alvarez, Raphaëlle Bazire, Pablo Rodríguez Del Río, Marta Vazquez-Ortiz, Joseph L. Baumert, Ronald Van Ree, Clare E.N. Mills, Montserrat Fernandez-Rivas, Jonathan O.B. Hourihane

Department of Food Science and Technology: Faculty Publications

Background: There is increasing interest in the use of eliciting doses (EDs) to inform allergen risk management. The ED can be estimated from the distribution of threshold doses for allergic subjects undergoing food challenges within a specified population. Estimated ED₀₅ values for cow's milk (the dose expected to cause objective allergic symptoms in 5% of the milk-allergic population) range from 0.5 mg to 13.9 mg cow's milk protein. We undertook a single-dose challenge study to validate a predicted ED₀₅ for cow's milk of 0.5 mg protein.

Methods: Participants were recruited from 4 clinical centres. Predetermined criteria were used …

Predicted Number Of Peanut-Allergic Patients Needed To Treat With Epicutaneous Immunotherapy (Epit) To Prevent One Allergic Reaction: A Novel Approach To Assessing Relevance, Benjamin C. Remington, Stef J. Koppelman, Todd D. Green, Gideon Lack, Graham Roberts, Dianne E. Campbell

Department of Food Science and Technology: Faculty Publications

Letter to the editor about the predicted number of peanut-allergic patients needed to treat with epicutaneous immunotherapy (EPIT) to prevent one allergic reaction, including a novel approach to assessing relevance.

Spatial Transferability Of Expert Opinion Models For American Beaver Habitat, Isidro Barela, Leslie M. Burger, Guiming Wang, Kristine O. Evans, Qingmin Meng, Jimmy D. Taylor

United States Department of Agriculture Wildlife Services: Staff Publications

Species distribution models and habitat suitability models (HSMs) have become a popular tool in the conservation of biodiversity. However, the ability to predict species spatial distributions at sites beyond the data source sites (i.e., spatial transferability) is critical for the applications of HSMs in the management and conservation of rare or endangered species. The main objective of our study was to assess the predictive performance and spatial transferability of expert opinion models (EOMs). To build EOMs, we identified through extensive literature reviews 17 key landscape variables to characterize habitat use by American beaver (Castor canadensis). We developed 31 pairwise opinion …

Evidence For Continental-Scale Dispersal Of Antimicrobial Resistant Bacteria By Landfill-Foraging Gulls, Christina A. Ahlstrom, Mariëlle L. Van Toor, Hanna Woksepp, Jeffrey C. Chandler, John A. Reed, Andrew B. Reeves, Jonas Waldenström, Alan B. Franklin, David C. Douglas, Jonas Bonnedahl, Andrew M. Ramey

United States Department of Agriculture Wildlife Services: Staff Publications

Anthropogenic inputs into the environment may serve as sources of antimicrobial resistant bacteria and alter the ecology and population dynamics of synanthropic wild animals by providing supplemental forage. In this study, we used a combination of phenotypic and genomic approaches to characterize antimicrobial resistant indicator bacteria, animal telemetry to describe host movement patterns, and a novel modeling approach to combine information fromthese diverse data streams to investigate the acquisition and long-distance dispersal of antimicrobial resistant bacteria by landfill-foraging gulls. Our results provide evidence that gulls acquire antimicrobial resistant bacteria from anthropogenic sources, which they may subsequently disperse across and between …

Food Habits Of Wintering Double-Crested Cormorants In The Mississippi Delta, Terrel W. Christie, Brian S. Dorr, J. Brian Davis, Luke A. Roy, Carole R. Engle, Katie Hanson-Dorr, Anita M. Kelly

United States Department of Agriculture Wildlife Services: Staff Publications

Given its ubiquity, it is not surprising that agriculture, including fin fish aquaculture, contributes to food webs worldwide and is used by numerous wildlife for foraging and meeting other needs. Double-crested Cormorants (Phalacrocorax auritus) impact United States commercial aquaculture and are considered the primary avian predator in catfish (Ictalurus spp.) aquaculture facilities in the Mississippi Delta. Recent changes in aquaculture practices, regulatory policies, and decreased overall hectares in production prompted this study that assessed cormorant consumption of catfish in relation to their night roosts through surveys and diet analysis. Cormorants were collected from night roosts from October …

Environmental Correlates Of Genetic Variation In The Invasive European Starling In North America, Natalie R. Hofmeister, Scott J. Werner, Irby J. Lovette

United States Department of Agriculture Wildlife Services: Staff Publications

Populations of invasive species that colonize and spread in novel environments may differentiate both through demographic processes and local selection. European starlings (Sturnus vulgaris) were introduced to New York in 1890 and subsequently spread throughout North America, becoming one of the most widespread and numerous bird species on the continent. Genome-wide comparisons across starling individuals and populations can identify demographic and/or selective factors that facilitated this rapid and successful expansion. We investigated patterns of genomic diversity and differentiation using reduced-representation genome sequencing of 17 winter-season sampling sites. Consistent with this species' high dispersal rate and rapid expansion history, …

Population Genomic Transformations Induced By Isolation Of Wild Bird Avian Influenza Viruses (Orthomyxoviridae) In Embryonated Chicken Eggs, Matthew W. Hopken, Antoinette J. Piaggio, K. L. Pabilonia, James Pierce, Theodore Anderson, Courtney Pierce, Zaid Abdo

United States Department of Agriculture Wildlife Services: Staff Publications

Isolation and cultivation of wild-type viruses in model organism cells or tissues is standard practice in virology. Oftentimes, the virus host species is distantly related to the species from which the culture system was developed. Thus, virus culture in these tissues and cells basically constitutes a host jump, which can lead to genomic changes through genetic drift and/or adaptation to the culture system. We directly sequenced 70 avian influenza virus (Orthomyxoviridae) genomes from oropharyngeal/cloacal swabs collected from wild bird species and paired virus isolates propagated from the same samples following isolation in specific-pathogen-free embryonated chicken eggs. The data were analyzed …