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2021

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Identification Of Snp Barcode For Classify Carbapenem-Resistant Enterobacteriaceae (Cre) And Non-Cre Using Nanopore Sequencing, Nicha Sangpiromapichai Jan 2021

Identification Of Snp Barcode For Classify Carbapenem-Resistant Enterobacteriaceae (Cre) And Non-Cre Using Nanopore Sequencing, Nicha Sangpiromapichai

Chulalongkorn University Theses and Dissertations (Chula ETD)

Background: The continuous increase of carbapenem-resistant Enterobacteriaceae (CRE) prevalence has been a serious problem for public health worldwide. In the previous study, 25% of 3864 Klebsiella pneumoniae isolates were carbapenem-resistant. One of the most important risks of bacterial drug resistance is the improper use of antibiotics. The routine antimicrobial-susceptibility test requires overnight and has a turnaround time of approximately 2–3 days which delays the proper antibiotics. This study aims to develop a technique to classify CRE and non-CRE by SNP barcode. Method: Forty Klebsiella pneumoniae (KP) clinical isolates were collected from 2020–2021 at King Chulalongkorn Memorial Hospital. Twenty samples were …

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Ultrastructural Changes Of Endothelial Cell, Pericyte And Vascular Basement Membrane In Cisplatin-Induced Neuropathy In Rats: Correlation With Neuropathic Changes After Treatments, Phetnarin Kobutree Jan 2021

Ultrastructural Changes Of Endothelial Cell, Pericyte And Vascular Basement Membrane In Cisplatin-Induced Neuropathy In Rats: Correlation With Neuropathic Changes After Treatments, Phetnarin Kobutree

Chulalongkorn University Theses and Dissertations (Chula ETD)

Cisplatin is an antineoplastic agent used to treat cancers of several organs. Peripheral neuropathy is one of its major side effects leading to dose reduction or cessation. Underlying mechanisms of cisplatin-induced neuropathy are not fully understood. According to current evidence, vascular dysfunction may play a role. Curcumin is a polyphenol found in the root of Curcuma longa with the anti-oxidant property and neuroprotection. Curcumin has shown effectiveness against experimental cisplatin neuropathy. B vitamins are a class of water-soluble vitamins that play important roles in cell metabolism and maintaining nervous system functions. Increasing evidence suggests the efficacy of vitamin B1-6-12 for …

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Study Of G6pd Deficiency With Clinical And Hematological Correlation In Newborn Infants, Thanaporn Pimpakan Jan 2021

Study Of G6pd Deficiency With Clinical And Hematological Correlation In Newborn Infants, Thanaporn Pimpakan

Chulalongkorn University Theses and Dissertations (Chula ETD)

G6PD deficiency is the most common enzymatic aberration, affecting more than a million people worldwide. If severe cases at birth are not diagnosed and treated promptly, they can develop into cerebral jaundice and hyperbilirubinemia. Hyperbilirubinemia is caused by an imbalance between increased bilirubin production and ineffective excretion of bilirubin. This can result in the neurotoxicity of bilirubin, kernicterus, and fatal mental retardation. Besides, It has been speculated that the presence of a high number of reticulocytes in newborns interferes with the diagnosis of G6PD deficiency since reticulocytes contain higher amounts of G6PD enzyme than mature erythrocytes. Therefore, the purposes of …

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Precise Extratemporal Pattern And Intramuscular Distribution Of The Facial Nerve, Sukanya Uruwan Jan 2021

Precise Extratemporal Pattern And Intramuscular Distribution Of The Facial Nerve, Sukanya Uruwan

Chulalongkorn University Theses and Dissertations (Chula ETD)

An arborization of the branches of the facial nerve has the most complexity and variability. Moreover, knowledge of the facial nerve has great importance for surgical and non-surgical interventions in the face region. However, few studies have attempted to determine the entire course and pattern of terminal branching of the facial nerve in the extratemporal and intramuscular parts of the face. Therefore, the purpose of this study was to investigate the precise detail of the facial nerve in extratemporal and intramuscular parts. This study was conducted in forty-two hemifaces of embalmed cadavers by conventional dissection, fourteen hemi-faces of embalmed cadavers …

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Effects Of Strongyloides Stercoralis Antigens On Immune Responses In Dendritic Cells, Vittawin Sutaveesup Jan 2021

Effects Of Strongyloides Stercoralis Antigens On Immune Responses In Dendritic Cells, Vittawin Sutaveesup

Chulalongkorn University Theses and Dissertations (Chula ETD)

Strongyloidiasis is a parasitic disease caused by Strongyloides stercoralis. Infection in the immunocompromised hosts such as organ transplants, SLE patients, or HIV and HIPV infected patients can cause severe strongyloidiasis. Infected patients have high levels of Th2, and Treg related cytokines, including IL-4, IL-5, IL-9, IL-10, IL-13, and TGF-β. These cytokines are then decreased to normal levels after the treatments. Parasites have several mechanisms to suppress or evade the host’s immune response, such as suppressing the expression of co-stimulatory molecules on antigen-presenting cells (APCs), stimulating apoptosis of APCs, or stimulating Th2 and Treg responses. Dendritic cells (DCs) play a crucial …

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An Anatomical Study Of The Central Myelin Portion And Transitional Zone Of The Oculomotor And Abducens Nerves, Wiyada Quanchareonsap Jan 2021

An Anatomical Study Of The Central Myelin Portion And Transitional Zone Of The Oculomotor And Abducens Nerves, Wiyada Quanchareonsap

Chulalongkorn University Theses and Dissertations (Chula ETD)

Neurovascular syndrome is a dysfunction of an individual cranial nerve which is compressed by vessels. Several neurovascular compression syndromes are well known such as trigeminal neuralgia, hemifacial spasm, and glossopharyngeal neuralgia. Having neurovascular conflicts at oculomotor nerves and abducens nerve cause ocular neuromyotonia and abducens nerve palsy. Site of compression is still controversial because of unclear definition. The term root entry/ exit zone (REZ) is defined variously and used interchangeably with transitional zone making it hard to compare between studies. It is generally thought to be at transitional zone which is defined as junction between central and peripheral nervous system. …

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A Pilot Study, Randomized Control Trial On Effect Of Oral Calcium Carbonate To Fecal Functional Levofloxacin Concentration In Healthy Volunteer Taking Oral Levofloxacin, Ophat Janphet Jan 2021

A Pilot Study, Randomized Control Trial On Effect Of Oral Calcium Carbonate To Fecal Functional Levofloxacin Concentration In Healthy Volunteer Taking Oral Levofloxacin, Ophat Janphet

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objective: We conducted a pilot randomized control trial (RCT) to study whether oral calcium carbonate (CaCO3) can lower fecal levofloxacin (LVX) concentration and preserve gut microbiota diversity in healthy volunteers. Methods: The healthy volunteers received a 5-day course of once-daily 500 mg LVX oral tablet and were randomly assigned to treatment (6-day course of 1,000 mg CaCO3 oral tablet twice daily) and control group (no CaCO3). The primary outcome was fecal LVX concentration by MIC and high-performance liquid chromatography (HPLC) on day 2 and 5. The secondary outcomes were fecal microbiota diversity Shannon index (H) by 16s rDNA analysis, plasma …

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Attenuation Of Chronic Kidney Disease (Ckd) And Uremia Induced Gut-Leakage By Lactobacillus Rhamnosus L34, A Probiotic Derived From Thai Population, In 5/6 Nephrectomy Model Mice; An Experimental Study, Somkanya Tungsanga Jan 2021

Attenuation Of Chronic Kidney Disease (Ckd) And Uremia Induced Gut-Leakage By Lactobacillus Rhamnosus L34, A Probiotic Derived From Thai Population, In 5/6 Nephrectomy Model Mice; An Experimental Study, Somkanya Tungsanga

Chulalongkorn University Theses and Dissertations (Chula ETD)

Background: Although pathogenic gut microbiota causes gut leakage, increases translocation of uremic toxins into circulation, and accelerates CKD progression, the local strain of Lactobacillus rhamnosus L34 (L34) might attenuate gut leakage. We explored the effects of L34 on kidney fibrosis and levels of gut-derived uremic toxins (GDUTs) in 5/6-nephrectomy (5/6 Nx) mice. Methods: At 6 weeks post-5/6 Nx in mice, either L34 (1x106 CFU) or phosphate buffer solution (as 5/6 Nx control) were daily fed for 14 weeks. In vitro, the effects of L34-conditioned media with or without indoxyl sulfate (a representative GDUT) on inflammation and cell integrity (transepithelial electrical …

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Effect Of Dielectric Barrier Discharge Plasma Hydrogenation On Oxidation Stability Of Biodiesel Derived From Vegetable Oils, Grittima Kongprawes Jan 2021

Effect Of Dielectric Barrier Discharge Plasma Hydrogenation On Oxidation Stability Of Biodiesel Derived From Vegetable Oils, Grittima Kongprawes

Chulalongkorn University Theses and Dissertations (Chula ETD)

A parallel-plate type dielectric barrier discharge (DBD) plasma was utilized to produce partially hydrogenated fatty acids methyl ester (PH-FAME) derived from soybean and palm FAME. PH-FAME exhibits improved oxidation resistance, resulting in a longer storage time and a delay in changing fuel properties. The DBD plasma reaction occurred at atmospheric pressure and ambient temperature without a catalyst. The best condition for 35 mL of soybean FAME hydrogenation was using 25% H2 at ambient temperature for 5.5 h. An increase in saturated and monounsaturated FAMEs corresponded to a reduction of iodine value from 128 to 67.4. This condition created trans fatty …

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Production Of Low Trans-Fatty Acid Margarine By Plasma Hydrogenation, Kunlanan Puprasit Jan 2021

Production Of Low Trans-Fatty Acid Margarine By Plasma Hydrogenation, Kunlanan Puprasit

Chulalongkorn University Theses and Dissertations (Chula ETD)

Margarine is a widely used raw material in the food and bakery industry. A successfully novel technique for refined palm olein hydrogenation to free trans-fat margarine production using non-thermal dielectric barrier discharge (DBD) plasma has been demonstrated. The new green method does not use chemicals as a catalyst, thus making it extremely environmentally friendly. This also performs well at room temperature and atmospheric pressure making it easier to manage. Two-electrode arrangement patterns were studied in this research: parallel plate and needle-in-tube. The results showed that using the parallel plate electrodes, 1.44% trans-fat (was detected) and a trans-fat generation rate of …

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The Effects Of A Recurrent Stroke Prevention Program On Blood Pressure, Low Density Lipoprotein Cholesterol, And Blood Glucose Among Patients With First Ischemic Stroke, Orapin Jullmusi Jan 2021

The Effects Of A Recurrent Stroke Prevention Program On Blood Pressure, Low Density Lipoprotein Cholesterol, And Blood Glucose Among Patients With First Ischemic Stroke, Orapin Jullmusi

Chulalongkorn University Theses and Dissertations (Chula ETD)

This cluster randomized control trial research with control group and repeated measure. The objectives of this study were to explore the effect of a recurrent stroke prevention program on blood pressure, low density lipoprotein cholesterol, and blood glucose among patients with first ischemic stroke. The participants were the patients who have been diagnosed with first ischemic stroke. They were recruited from two stroke units from two tertiary hospitals in public health region 2, Thailand. Sixty participants were randomly recruited and divided into experimental group (n=30) and control group (n=30). The participants in the experimental group received the recurrent stroke prevention …

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Predictors Of Time To Hospital Among People With Colorectal Cancer, Saimai Tumwijit Jan 2021

Predictors Of Time To Hospital Among People With Colorectal Cancer, Saimai Tumwijit

Chulalongkorn University Theses and Dissertations (Chula ETD)

This retrospective, cross-sectional study aims to describe time to hospital, relating factors, and determine predictors of time to hospital among people with colorectal cancer. Multistage random sampling was used to yield a probability of 240 people with colorectal cancer visiting medical and surgical outpatient departments, oncology units, radiology units, endoscopic units, and inpatient departments. Research instruments consisted of personal information sheet, time to colorectal cancer diagnosis questionnaire, knowledge about colorectal cancer questionnaire (KR-20 = .786), The modified illness perception questionnaire-revised (a = .674 - .836), health care seeking behavior questionnaire (a = .706), and perceived seriousness of warning signs and …

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Effect Of Different Placement Methods On Voids Formationin Class Ii Cavity Restored With Bulk-Fill Resin Composite, Parinthorn Siyoputhawong Jan 2021

Effect Of Different Placement Methods On Voids Formationin Class Ii Cavity Restored With Bulk-Fill Resin Composite, Parinthorn Siyoputhawong

Chulalongkorn University Theses and Dissertations (Chula ETD)

The aim of this study was to evaluate void formation by micro-computed tomography in two-surface Class II cavities restored with two bulk-fill resin composites using different placement methods. Standardized Class II cavities were prepared in forty intact human maxillary first premolar teeth. The teeth were randomly divided into four groups and restored with (n=10): 1) one bulk placement with hand instrument; 2) two-bulk placement with hand instrument; 3) one bulk placement with injectable dispenser; 4) one bulk placement with injectable dispenser and sonic-activated handpiece. Percentage of void formation in the entire restoration was evaluated. Welch's ANOVA and Games-Howell post hoc …

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Factors Influencing The Dentist’S Decision To Propose A Tooth Autotransplantation In The Faculty Of Dentistry, Chulalongkorn University, Thunchanok Sinprasurdsook Jan 2021

Factors Influencing The Dentist’S Decision To Propose A Tooth Autotransplantation In The Faculty Of Dentistry, Chulalongkorn University, Thunchanok Sinprasurdsook

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objectives: To study factors which influence a dentist’s decision to propose the Tooth Autotransplantation (AT). Material and methods: A cross-sectional study was conducted among 99 dentists between January and March 2021. A questionnaire comprised demographic characteristics, unprimed scenario, primed scenario, reasoning behind decisions, experience, and knowledge of AT. Data were analyzed using the Chi-square test, McNemar’s test, and multiple logistic regression. Results: The respondents comprised 73 females and 26 males with a mean age of 30.84 ± 6.238 years. In the unprimed scenario, there were significant associations between fields of expertise, experience, knowledge of current indications, outcomes, and the benefits …

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Role Of Extracellular Adenosine Triphosphate (Eatp) On Immunomodulatory Function Of Human Periodontal Ligament Cells, Maythwe Kyawsoewin Jan 2021

Role Of Extracellular Adenosine Triphosphate (Eatp) On Immunomodulatory Function Of Human Periodontal Ligament Cells, Maythwe Kyawsoewin

Chulalongkorn University Theses and Dissertations (Chula ETD)

Background: Adenosine triphosphate, a nucleotide that acts as an important extracellular messenger, is released into the extracellular environment during various physiologic and pathological conditions. Human periodontal ligament cells (hPDLCs) can release adenosine triphosphate into the extracellular environment in response to mechanical stress. Extracellular adenosine triphosphate (eATP) plays role in both inflammation and osteogenic differentiation processes. eATP also participates in immunosuppressive action on immune cells. However, the role of eATP on the immunomodulatory function of hPDLCs is still unclear. This study aims to investigate the effects of eATP on the immunomodulatory function of hPDLCs and the participation of specific purinergic P2 …

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Anti-Inflammatory And Regeneration Properties Of Iloprost On Inflamed Human Dental Pulp Cells Model: An In Vitro Study, Rahman Wahyudi Jan 2021

Anti-Inflammatory And Regeneration Properties Of Iloprost On Inflamed Human Dental Pulp Cells Model: An In Vitro Study, Rahman Wahyudi

Chulalongkorn University Theses and Dissertations (Chula ETD)

In the clinical situation, pulp exposure can happen during caries removal procedures. Inflammation can occur on the pulp of the exposed area because of infiltration of bacteria from caries or as a result of pulp reaction to caries removal drilling. When the pulp was exposed during the caries removal procedure, traditional methods to preserve the pulp such as pulp capping and pulpotomy were performed. The ideal treatment outcome of the pulp exposure is to regain the primary structure of tubular dentin as well as maintain the vitality and healthiness of the dental pulp Thus, good dental materials or drugs used …

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Effect Of Shear Force On Immunosuppressive Property Of Human Periodontal Ligament Cells, Ravipha Suwittayarak Jan 2021

Effect Of Shear Force On Immunosuppressive Property Of Human Periodontal Ligament Cells, Ravipha Suwittayarak

Chulalongkorn University Theses and Dissertations (Chula ETD)

The immunomodulatory property is to regulate immune cell function. IDO and TGF-β1 inhibited T cell proliferation and induced regulatory T (Treg) cells differentiation. However, this property must be activated by inflammation. Recently, mechanical forces have also activated this property. Hence, this study investigated effect of shear stress on immunosuppressive property of hPDL cells. Cells were subjected to shear stress then expression of IDO, TGF-β1, IFN-gamma and COX2 mRNA and protein was examined by qRT-PCR, ELISA, and IDO activity. Conditioned media derived shear stress were treated with CD4+ T cells. The proliferative T cell effect was determined using resazurin. The FOXP3, …

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Efficacy Of Cannabidiol (Cbd) For The Treatment Of Recurrent Aphthous Ulcers, Chalapinyo Umpreecha Jan 2021

Efficacy Of Cannabidiol (Cbd) For The Treatment Of Recurrent Aphthous Ulcers, Chalapinyo Umpreecha

Chulalongkorn University Theses and Dissertations (Chula ETD)

The objectives of this research were 1) To investigate the allergic reactions to cannabidiol (CBD) when used on human skin. 2) To assess local and systemic side effects of CBD when used on normal oral mucosa. 3) To compare the efficacy among 0.1% CBD, 0.1% triamcinolone acetonide (TA), and placebo (pure oral paste) in recurrent aphthous ulcer (RAU) treatment. This project was composed of 3 phases. Phase 1, a skin patch test was performed on 100 healthy subjects. Phase 2, CBD was applied to normal oral mucosa of 50 healthy subjects 3 times/day for 7 days. Oral examination, vital signs, …

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Effects Of Local Versus Systemic Administration Of Cxcr4 Inhibitor Amd3100 On Orthodontic Tooth Movement In Rats, Narubhorn Ongprakobkul Jan 2021

Effects Of Local Versus Systemic Administration Of Cxcr4 Inhibitor Amd3100 On Orthodontic Tooth Movement In Rats, Narubhorn Ongprakobkul

Chulalongkorn University Theses and Dissertations (Chula ETD)

Chemokines play pivotal roles in orthodontic tooth movement (OTM) through osteoclast-mediated bone resorption, but the underlying mechanism remains unclear. We aimed to elucidate the effects of serial local vs systemic administration of the chemokine receptor CXCR4 antagonist AMD3100 on OTM. The maxillary right first molar (M1) of rats was moved mesially using a 10 g of force nickel-titanium coil spring. The injections were performed every other day with phosphate-buffered saline as a control, whereas local and systemic animals were injected with AMD3100 at the buccal palatal mucosa adjacent to M1 and subcutaneously, respectively. OTM distance and alveolar bone were examined …

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Differences Of Sound And Morphology In Skeletal Class Iii Patients, Natthaporn Pravitharangul Jan 2021

Differences Of Sound And Morphology In Skeletal Class Iii Patients, Natthaporn Pravitharangul

Chulalongkorn University Theses and Dissertations (Chula ETD)

[Background] This study aimed to explore differences in vowel production using acoustic analysis in skeletal Class III and Class I Japanese participants, and to investigate the correlation between vowel sounds and cephalometric variables in skeletal Class III subjects. [Materials and Methods] Japanese males with skeletal Class III (ANB<0°) and Class I skeletal relationships (0.62°

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The Designs Of Miniscrew-Assisted Transpalatal Arch Affect Molar Intrusion, Ornnicha Pooktuantong Jan 2021

The Designs Of Miniscrew-Assisted Transpalatal Arch Affect Molar Intrusion, Ornnicha Pooktuantong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Abstract: An anterior open bite is one of the most difficult malocclusions in orthodontic treatment. For such malocclusion, orthodontic miniscrews insertion into both buccal and palatal alveolar regions has been indicated for molar intrusion, but it involves a risk of tooth root injury. To solve the problem, midpalatal miniscrew-attached extension arm (MMEA) is adopted. However, this method causes palatal tipping of the molar because intrusive loads were applied only from palatal side. Currently, transpalatal arch is added to avoid tipping movement, but it induces patient's discomfort. Hence, the objective of this study was to evaluate the loading conditions for maxillary …

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Effects Of Intrauterine Intermittent Hypoxia On Skeletal Muscle In Offspring Rats, Wirongrong Wongkitikamjorn Jan 2021

Effects Of Intrauterine Intermittent Hypoxia On Skeletal Muscle In Offspring Rats, Wirongrong Wongkitikamjorn

Chulalongkorn University Theses and Dissertations (Chula ETD)

Gestational intermittent hypoxia (IH), a hallmark of OSA, alters the offspring's respiratory neural control and diaphragm contractile function. Thus, we aimed to investigate the effects of gestational IH on the muscle development and metabolism of geniohyoid (GH), which is innervated by the respiratory-related hypoglossal nerve and plays a role in tongue traction and suckling, in male offspring rats compared with masseter (MAS), the largest masticatory muscle. Pregnant Sprague-Dawley rats were exposed to IH (3-min periods of 4–21% O2) compared to Normoxia for 8 hours/day during gestational days 7–20. GH and MAS from 35-day-old male offspring (n = 6 /group) were …

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Cellular Responses Of Stem Cells Isolated From Human Exfoliated Deciduous Teeth Towards Different Concentration Of Calcium Ion, Thanika Phlinyos Jan 2021

Cellular Responses Of Stem Cells Isolated From Human Exfoliated Deciduous Teeth Towards Different Concentration Of Calcium Ion, Thanika Phlinyos

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objectives: The purpose of this study was to investigate the effect of Ca2+ on proliferation, osteogenic differentiation, and migration of stem cells from human exfoliated teeth (SHEDs) in vitro Materials and methods: SHEDs were seeded in culture media and osteogenic induction media containing 1.8-16.2 mM of Ca2+. SHEDs proliferation was determined using MTT assay and colony forming unit assay. Osteogenic differentiation was evaluated using mineralization assay and osteogenic marker gene expression and cell migration was evaluated using wound healing assay. Values were expressed as mean + S.D. Statistical analysis of MTT assay and wound healing assay were performed using two-way …

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Expression Of Vascular Endothelial Growth Factor Protein In Mrna-Transfected Human Periodontal Ligament Cells, Mana Naratippakorn Jan 2021

Expression Of Vascular Endothelial Growth Factor Protein In Mrna-Transfected Human Periodontal Ligament Cells, Mana Naratippakorn

Chulalongkorn University Theses and Dissertations (Chula ETD)

The complete regeneration of periodontal tissues following current periodontal therapy remain challenging and unpredictable. Nucleoside-modified messenger RNA (mRNA) technology can be a promising novel platform in regenerative medicine. The aims of this study were to evaluate whether pseudouridine modified mRNA encoding vascular endothelial growth factor (VEGF) could induce VEGF production in human periodontal ligament cells (PDLCs) and this translated protein function by promoting in vivo blood vessel formation using chorioallantoic membrane (CAM) assay. Isolated PDLCs from healthy periodontal tissue were transfected with modified mRNA encoding VEGF (VEGF mRNA) complexed with a transfecting agent, Lipofectamine 2000 (L2000) and L2000 alone (control). …

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Clinical Efficacy Of Silk Sericin Dressing With Collagen For Split-Thickness Skin Graft Donor Site Treatment, Apirujee Punjataewakupt Jan 2021

Clinical Efficacy Of Silk Sericin Dressing With Collagen For Split-Thickness Skin Graft Donor Site Treatment, Apirujee Punjataewakupt

Chulalongkorn University Theses and Dissertations (Chula ETD)

Sericin dressing containing collagen hydrolysate (SDC) was evaluated in this study, consisting of two phases of clinical trials. The clinical safety of SDC was determined using a patch test in 103 healthy volunteers (phase I). Most of the results (around 90%) in the SDC group were interpreted as negative and doubtful reactions, and the remaining results (around 10%) were weak reactions. After that, the clinical efficacy and safety of SDC were investigated in 21 patients with 30 split-thickness skin graft (STSG) donor sites (phase II). Each donor site was divided into two equal sites. SDC or the commercial dressing (Bactigras®) …

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Production Optimization Of Porcine Reproductive And Respiratory Syndrome (Prrs) Virus Nucleocapsid Protein In Nicotiana Benthamiana, Boonyasit Porngarm Jan 2021

Production Optimization Of Porcine Reproductive And Respiratory Syndrome (Prrs) Virus Nucleocapsid Protein In Nicotiana Benthamiana, Boonyasit Porngarm

Chulalongkorn University Theses and Dissertations (Chula ETD)

Porcine Reproductive and Respiratory Syndrome virus (PRRSV) is a considerable pathogen to occur disease in pigs and effect to economic on the swine industry around the world including Thailand. Routine observation, rapid diagnosis of PRRSV infection is essential for effective monitoring and disease control. The serological assays using highly conserved viral nucleocapsid (N) protein are widely used for the detection of antibodies to the PRRSV. Accordingly, this study was aimed to produce the N-protein of the PRRSV in the plant system by using Nicotiana benthamiana. The N-protein gene was designed into four constructs that contained with and without the plant …

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Antioxidant Activity Of Thunbergia Laurifolia Lindl. Ethanolic Extract In Keratinocytes, Buakotchaphan Jirabanjerdsiri Jan 2021

Antioxidant Activity Of Thunbergia Laurifolia Lindl. Ethanolic Extract In Keratinocytes, Buakotchaphan Jirabanjerdsiri

Chulalongkorn University Theses and Dissertations (Chula ETD)

Fine particulate matter (PM2.5) can cause skin damage through the induction of oxidative stress in epidermis. Antioxidants help counteract with cellular oxidant species and maintain cell homeostasis. This study aims to examine the protective effect of Thunbergia laurifolia lindl. ethanolic extract on PM2.5-mediated oxidative stress in epidermal keratinocytes. High-performance liquid chromatography with diode array detection was conducted for quantitative analysis of rosmarinic acid content in the extract. Cell viability was detected by the 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide assay. Intracellular ROS was monitored using 2’,7’-Dichlorodihydrofluorescein diacetate. Proteins related to p62-Keap1-Nrf2 signaling pathway were examined by western blot analysis. Expressions of Nrf2 and p62 …

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Antioxidant With Antimelanogenic Activities Of Sericin Hydrolysates Optimized By Response Surface Methodology In Human Melanin-Generating Mnt-1 Cells, Keerati Joyjamras Jan 2021

Antioxidant With Antimelanogenic Activities Of Sericin Hydrolysates Optimized By Response Surface Methodology In Human Melanin-Generating Mnt-1 Cells, Keerati Joyjamras

Chulalongkorn University Theses and Dissertations (Chula ETD)

Sericin, a protein presenting in the wastewater from silk industry, causes water pollution and ecological problem. To increase economic value to this waste product, the optimum enzymatic condition that could create sericin hydrolysates with high antioxidant and antimelanogenic capacities was generated through response surface methodology (RSM). Response surface plots demonstrate the major role of temperature on scavenging capacity of sericin hydrolysates assessed via DPPH, FRAP and ORAC assays. Alcalase®-hydrolyzed sericin consisted of sericin-related peptides in differing amounts and smaller sizes compared with unhydrolyzed sericin, as respectively demonstrated by peptidomic and SDS-PAGE analysis. Pre-incubation with 20 mg/mL sericin hydrolysates digested by …

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Development Of Plant-Produced Subunit Vaccine And Therapeutic Protein For Covid-19, Konlavat Siriwattananon Jan 2021

Development Of Plant-Produced Subunit Vaccine And Therapeutic Protein For Covid-19, Konlavat Siriwattananon

Chulalongkorn University Theses and Dissertations (Chula ETD)

Due to the rapid transmission of the Coronavirus Disease 2019 (COVID-19) causing serious public health problems and economic burden, the development of effective therapeutic interventions is urgently needed for controlling the ongoing pandemic disease. In this study, we have transiently produced recombinant SARS-CoV-2 RBD and human ACE2 fused with the Fc region of human IgG1 in Nicotiana benthamianain in order to use as a preventive subunit vaccine and therapeutic against SARS-CoV-2. The recombinant SARS-CoV-2 RBD-Fc subunit vaccine was formulated with different commercially available adjuvants including aluminium hydroxide gel (Alum), AddaVaxTM (MF59), monophosphoryl lipid A from Salmonella Minnesota R595 (mPLA-SM), and …

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Taxonomy And Biological Activities Of A Novel Saccharothrix Species From Soil Of Sichang Island, Md Abul Kalam Azad Jan 2021

Taxonomy And Biological Activities Of A Novel Saccharothrix Species From Soil Of Sichang Island, Md Abul Kalam Azad

Chulalongkorn University Theses and Dissertations (Chula ETD)

Actinobacteria are the sources of bioactive natural products. Rare actinobacteria are the less frequently isolated genera of actinobacteria. During an investigation of diversity of rare actinobacteria, actinobacteria isolate SC076T was isolated from a soil sample collected from Sichang Island, Chonburi Province, Thailand. The strain showed the highest 16S rRNA gene similarity to Saccharothrix australiensis DSM 43800T (98.58%) and Saccharothrix espanaensis DSM 44229T (98.58%). The zigzag morphology of the spore chain was observed on the aerial mycelia. meso-diaminopimelic acid was detected in the peptidoglycan. Whole-cell sugars contained rhamnose, ribose, mannose glucose and galactose. Polar lipids are phosphatidylethanolamine, hydroxyphosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol …

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