Characterization Of Micronutrient Dependent Growth By Several Temperate Freshwater Phytoplankton, Purnank Shah

Theses and Dissertations (Comprehensive)

Harmful algal blooms (HABs) are a growing problem in many freshwater waterbodies in Canada and around the world. HABs have wide-ranging impacts to ecosystems and economies. Since the 1970s, the primary focus of policies with respect to curbing HABs has been to lowering the inputs of P into waters. However, reports of HABs continue to increase even with all these P-removing policies in place. Some jurisdictions have chosen to focus on N-removal instead or in conjunction with P-removal to help with HABs. However, because some cyanobacteria have the ability to fix atmospheric N₂,N-removal might only lead to the …

Composition And Homology In The Taxonomic Classification Of Escherichia Coli, Tanya Irani

Theses and Dissertations (Comprehensive)

As new techniques have been introduced, specifically the possibility of complete genome sequencing, better methods of defining bacterial species have also been proposed. One of the most recently proposed methods, using bioinformatic techniques, is to calculate the average nucleotide identity (ANI) between the homologous genome segments of different isolates. Another method for species discrimination that has been tested successfully is the similarity of DNA compositional signatures. However, in a recent update, DNA signatures split the available Escherichia coli complete genomes into three groups. To check if this result was consistent with such genomes belonging to different species, we tested methods …

To Like Or To Want? Exploring The Psychobiological Processes Of Human Food Reward, Angela Mastroianni

Theses and Dissertations (Comprehensive)

The Incentive Salience Theory (IST; Berridge and Robinson, 1998; 2016) has shown that wanting (craving) and liking (affective) processes can affect eating behaviour differently in animals. These processes often correlate but can dissociate (change differently) under certain circumstances as they rely on separate neural mechanisms (Berridge, 1996). More recently, work has been testing the IST in people by comparing broad food categories (Finlayson et al. 2007a; 2008). The results have been inconsistent, sparking debate about the theory's value for studying human feeding (Havermans, 2011; 2012).

This dissertation's aim was to explore if IST wanting and liking are independent processes controlling …

Decipher The Effect Of Gamification In Harnessing Boredom And Improving Performance, Zhuoyi Zhao

Theses and Dissertations (Comprehensive)

My thesis investigates the effectiveness of gamification in harnessing boredom and improving performance in a repetitive work process. In video games, “loot” rewards are unpredictable, intermittent gains used to motivate players to repeat boring actions. In a 2 X 2 experiment, I examine how loot rewards in point form may impact 1) disengagement, which is an immediate outcome of boredom, and 2) performance in settings where the points have and do not have cash value, respectively. More specifically, I manipulate the level of point reward unpredictability (fixed versus loot) and whether point rewards have cash value (absent versus present).

In …

A Bioinformatic Analysis Of The Biosynthesis Of Carotenoids In The Copepod Tigriopus Californicus, Anchalya Balasubramaniam

Theses and Dissertations (Comprehensive)

Abstract

Biological pigments, also called biochromes, are coloured compounds which are displayed by a variety of life forms, including animals, due to selective colour absorption. The combination of light absorption and reflection enables each pigment to portray a distinct colour which results in the broad spectrum of colours we observe in our surroundings. Carotenoids are a large group of yellow, orange, and red biological pigments found in living organisms. Our current biomolecular knowledge of carotenoids is heavily derived from studying the pathway in photosynthetic prokaryotes, bacteria, fungi, and plants. Carotenoid pigments are exceptionally multifunctional as they act as photo-protectors against …

Wildlife Forage Recovery Following Boreal Wildfire, Alexis Jorgensen, Jennifer Baltzer

Theses and Dissertations (Comprehensive)

Climate change is altering the boreal wildfire regime through increases in the extent and severity of burning and reductions in fire return intervals. These changes can alter the regeneration trajectory of canopy species and ground vegetation, with implications for wildlife habitat. There is some uncertainty about the timelines of when different animal species will use burned areas as their preferred forage taxa recover following fire, and how such recovery is mediated by environmental factors. Here, we aim to address these knowledge gaps through the following questions: 1) What are the main forage types consumed by boreal wildlife and how much …

"Learning" To Feel Valued: Exploring The Impact Of Community Service Learning On Student Mattering And Mental Health, Emily Weatherhead

Theses and Dissertations (Comprehensive)

Post-secondary students are facing an unprecedented level of mental health concerns. While treating mental illness has traditionally been prioritized, mental health promotion strategies are also essential. Existing research has shown that mattering, one’s ability to feel valued by and add value to their community, can promote positive mental health in post-secondary students. Using mixed methods, the goal of this research was to explore the potential of community service learning (CSL), which allows students to participate in community placements in exchange for course credit, to increase student mattering and positive mental health. Pre- and post-online surveys were administered to a sample …

The Combined Transitions Of Great Power Politics And The Global Energy System A Comparative Analysis Of China, The United States And Russia, Roger Boyd

Theses and Dissertations (Comprehensive)

The objective of this study is to provide a framework that allows for a holistic and integrated analysis of what will become, if they are not already, the two most significant determinants of foreign policy – the need for a fundamental change in the global energy system and the nature of major power competition within the international system. It specifically rejects the eco-modernist framing of the required energy transition as simply a technocratic challenge, and instead accepts the large-scale nature of the social, political and economic changes and disruptions that will be required. By framing these changes within the dynamic …

Investigating The Antitumor Effects Of A Dsrna-Nanoparticle Complex In An In Vitro Ovarian Cancer Model, Aaron Lewis

Theses and Dissertations (Comprehensive)

An estimated 1 in 70 women will be diagnosed with ovarian cancer in their lifetime. Despite advanced detection and treatment methods, it remains a silent killer with an expected survival rate of 50%. A developing method in cancer treatment is the use of compounds that stimulate the immune system to aid in the body's fight against the disease. This project focused on the use of the potent immune stimulant double-stranded RNA (dsRNA), commercially available as polyinosinic:polycytidylic acid, poly(I:C), to induce cytotoxicity in two ovarian cancer cell lines; SKOV-3 and OVCAR-3. Some challenges exist with the delivery of dsRNA due to …

Addressing Urgent Questions For Pfas In The 21st Century, Carla Ng, Ian T. Cousins, Jamie C. Dewitt, Juliane Glüge, Gretta Goldenman, Dorte Herzke, Rainer Lohmann, Mark Miller, Sharyle Patton, Martin Scheringer, Xenia Trier, Zhanyun Wang

Graduate School of Oceanography Faculty Publications

Despite decades of research on per- and polyfluoroalkyl substances (PFAS), fundamental obstacles remain to addressing worldwide contamination by these chemicals and their associated impacts on environmental quality and health. Here, we propose six urgent questions relevant to science, technology, and policy that must be tackled to address the “PFAS problem”: (1) What are the global production volumes of PFAS, and where are PFAS used? (2) Where are the unknown PFAS hotspots in the environment? (3) How can we make measuring PFAS globally accessible? (4) How can we safely manage PFAS-containing waste? (5) How do we understand and describe the health …

Information Requirements Under The Essential-Use Concept: Pfas Case Studies, Juliane Glüge, Rachel London, Ian T. Cousins, Jamie Dewitt, Gretta Goldenman, Dorte Herzke, Rainer Lohmann, Mark Miller, Carla A. Ng, Sharyle Patton, Xenia Trier, Zhanyun Wang, Martin Scheringer

Graduate School of Oceanography Faculty Publications

Per- and polyfluoroalkyl substances (PFAS) are a class of substances for which there are widespread concerns about their extreme persistence in combination with toxic effects. It has been argued that PFAS should only be employed in those uses that are necessary for health or safety or are critical for the functioning of society and where no alternatives are available (“essential-use concept”). Implementing the essential-use concept requires a sufficient understanding of the current uses of PFAS and of the availability, suitability, and hazardous properties of alternatives. To illustrate the information requirements under the essential-use concept, we investigate seven different PFAS uses, …

Finding Essentiality Feasible: Common Questions And Misinterpretations Concerning The “Essential-Use” Concept, Ian T. Cousins, Jamie C. De Witt, Juliane Glüge, Gretta Goldenman, Dorte Herzke, Rainer Lohmann, Mark Miller, Carla A. Ng, Sharyle Patton, Martin Scheringer, Xenia Trier, Zhanyun Wang

Graduate School of Oceanography Faculty Publications

The essential-use concept is a tool that can guide the phase-out of per- and polyfluoroalkyl substances (PFAS) and potentially other substances of concern. This concept is a novel approach to chemicals management that determines whether using substances of concern, such as PFAS, is truly essential for a given functionality. To assess the essentiality of a particular use case, three considerations need to be addressed: (1) the function (chemical, end use and service) that the chemical provides in the use case, (2) whether the function is necessary for health and safety and critical for the functioning of society and (3) if …

An Operational Overview Of The Export Processes In The Ocean From Remote Sensing (Exports) Northeast Pacific Field Deployment, Melissa M. Omand, Bethany D. Jenkins, Heather Mcnair, Tatiana A. Rynearson, Et Al

Graduate School of Oceanography Faculty Publications

The goal of the EXport Processes in the Ocean from RemoTe Sensing (EXPORTS) field campaign is to develop a predictive understanding of the export, fate, and carbon cycle impacts of global ocean net primary production. To accomplish this goal, observations of export flux pathways, plankton community composition, food web processes, and optical, physical, and biogeochemical (BGC) properties are needed over a range of ecosystem states. Here we introduce the first EXPORTS field deployment to Ocean Station Papa in the Northeast Pacific Ocean during summer of 2018, providing context for other papers in this special collection. The experiment was conducted with …

Differential Patterns Of Connectivity In Western Pacific Hydrothermal Vent Metapopulations: A Comparison Of Biophysical And Genetic Models, Corinna Breusing, Shannon B. Johnson, Satoshi Mitarai, Roxanne A. Beinart, Verena Tunnicliffe

Graduate School of Oceanography Faculty Publications

Hydrothermal ecosystems face threats from planned deep-seabed mining activities, despite the fact that patterns of realized connectivity among vent-associated populations and communities are still poorly understood. Since populations of vent endemic species depend on larval dispersal to maintain connectivity and resilience to habitat changes, effective conservation strategies for hydrothermal ecosystems should include assessments of metapopulation dynamics. In this study, we combined population genetic methods with biophysical models to assess strength and direction of gene flow within four species of the genus Alviniconcha (A. boucheti, A. kojimai, A. strummeri and A. hessleri) that …

Menthol To Induce Non-Shivering Thermogenesis Via Trpm8/Pka Signaling For Treatment Of Obesity, Owen Sanders, Jayalekshmi Rajagopalan, Lekshmy Rajagopal

Biology Faculty Publications and Presentations

Increasing basal energy expenditure via uncoupling protein 1 (UCP1)-dependent non-shivering thermogenesis is an attractive therapeutic strategy for treatment of obesity. Transient receptor potential melastatin 8 (TRPM8) channel activation by cold and cold mimetics induces UCP1 transcription and prevents obesity in animals, but the clinical relevance of this relationship remains incompletely understood. A review of TRPM8 channel agonism for treatment of obesity focusing on menthol was undertaken. Adipocyte TRPM8 activation results in Ca2+ influx and protein kinase A (PKA) activation, which induces mitochondrial elongation, mitochondrial localization to lipid droplets, lipolysis, β-oxidation, and UCP1 expression. Ca2+-induced mitochondrial reactive oxygen species activate UCP1. …

Lyme Disease Essentials For Healthcare Workers, Donita Blanken-Little

DNP Research Projects

No abstract provided.

Metaphors Of International Law, Harlan G. Cohen

Faculty Scholarship

This chapter explores international law in search of its hidden and not-so-hidden metaphors. In so doing, it discovers a world inhabited by states, where rules are mined or picked when ripe, where trade keeps boats forever afloat on rising tides. But is also unveils a world in which voices are silenced, inequality is ignored, and hands are washed of responsibility.

International law is built on metaphors. Metaphors provide a language to describe and convey the law’s operation, help international lawyers identify legal subjects and categorize situations in doctrinal categories, and provide normative justifications for the law. Exploring their operation at …

Genomic Diversity Of Salmonella Enterica -The Uowucc 10k Genomes Project, Mark Achtman, Zhemin Zhou, Nabil Fareed Alikhan, William Tyne, Julian Parkhill, Martin Cormican, Chien Shun Chiou, Mia Torpdahl, Eva Litrup, Deirdre M. Prendergast, John E. Moore, Sam Strain, Christian Kornschober, Richard Meinersmann, Alexandra Uesbeck, François Xavier Weill, Aidan Coffey, Helene Andrews-Polymenis, Roy Curtiss, Séamus Fanning

Department of Biological Sciences Publications

Background: Most publicly available genomes of Salmonella enterica are from human disease in the US and the UK, or from domesticated animals in the US. Methods: Here we describe a historical collection of 10,000 strains isolated between 1891-2010 in 73 different countries. They encompass a broad range of sources, ranging from rivers through reptiles to the diversity of all S. enterica isolated on the island of Ireland between 2000 and 2005. Genomic DNA was isolated, and sequenced by Illumina short read sequencing. Results: The short reads are publicly available in the Short Reads Archive. They were also uploaded to EnteroBase, …

In/Voluntary Surrogacy In Genesis, David J. Zucker

The Asbury Journal

This article re-examines the issue of surrogacy in Genesis. It proposes some different factors, and questions some previous conclusions raised by other scholars, and especially examining feminist scholars approaches to the issue in the cases of Hagar/Abraham (and Sarah), and Bilhah-Zilpah/Jacob (and Rachel, Leah). The author examines these cases in the light of scriptural evidence and the original Hebrew to seek to understand the nature of the relationship of these complex characters. How much say did the surrogates have with regard to the relationship? What was their status within the situation of the text, and how should we reflect on …

Journal In Entirety

The Asbury Journal

No abstract provided.

Drunk In The Spirit: Eschatology, Soteriology, And Ecclesiology, Matt Ayars

The Asbury Journal

There are a number of New Testament passages that associate the concepts of the new age of the Spirit, believer unity, covenant membership, and indwelling of the Holy Spirit with the symbols of the temple, Christ’s body, the Eucharist, table fellowship, and drinking wine. This article explores these associations and their theological implications with particular attention lent to the metaphor of drinking the Holy Spirit and its implications for soteriology, ecclesiology, and eschatology.

Journal In Entirety

The Asbury Journal

No abstract provided.

Lincoln Among The Methodists, Samuel J. Rogal

The Asbury Journal

While not a professed member of any religious denomination, the relationship between Abraham Lincoln and the Methodist Episcopal Church of his time is important, both in terms of their views on the abolition of slavery and the political rise of the number of Methodists in the United States. This article charts the course of that relationship from before Lincoln’s Presidency, his election campaign against Peter Cartwright ... this was when the rise of Methodism was to have serious implications politically because of thebrapid size, growth, and moral views of the church.

A Model Of Mission By And For The Marginalized: Cezms Missions To The Deaf, Thomas Hampton

The Asbury Journal

This paper shares the untold stories of three missions to the deaf and mute in India and Sri Lanka. Prior to Indian independence, three of the first schools in the country focused in the deaf were founded by the women of the Church of England Zenana Missionary Society (CEZMS). Their locations were Palamcottah, in Tirunelveli, India, Mylapore, in Chennai, India, and Kaitadi, near Jaffna, Sri Lanka. Each of these schools has adapted over time and continues to serve deaf students today with local leadership. there is a wider interest in the factors which allowed missionary institutions to transition to local …

Focus Stacking Images Of Morphological Character States For Differentiating The Adults Of Ixodes Affinis And Ixodes Scapularis (Acari: Ixodidae) In Areas Of Sympatry, Robyn M. Nadolny, Marcée Toliver, Holly D. Gaff, John G. Snodgrass, Richard G. Robbins

Biological Sciences Faculty Publications

Adult females and males of Ixodes affinis and Ixodes scapularis are illustrated by focus stacking image photography, and morphological character states are described that reliably differentiate the two species. In conjunction with other environmental cues, such as the questing phenology of adults, these characteristics will enable the rapid identification of adults of either sex along the southern Coastal Plain of the United States, where these species are sympatric.

Dietary Protein Content And Digestibility Influences Discrimination Of Amino Acid Nitrogen Isotope Values In A Terrestrial Omnivorous Mammal, John P. Whiteman, Mauriel Rodriguez Curras, Kelli L. Feeser, Seth D. Newsome

Biological Sciences Faculty Publications

RATIONALE: Ecologists increasingly determine the δ¹⁵N values of amino acids (AA) in animal tissue; "source" AA typically exhibit minor variation between diet and consumer, while "trophic" AA have increased δ¹⁵N values in consumers. Thus, trophic-source δ¹⁵N offsets (i.e., Δ¹⁵N_T-S) reflect trophic position in a food web. However, even minor variation in δ¹⁵N_{source AA} values may influence the magnitude of offset that represents a trophic step, known as the trophic discrimination factor (i.e., TDF_T-S). Diet digestibility and protein content can influence the δ¹⁵N values …

Tick Extracellular Vesicles Enable Arthropod Feeding And Promote Distinct Outcomes Of Bacterial Infection, Adela S. Oliva Chávez, Xiaowei Wang, Liron Marnin, Nathan K. Archer, Holly L. Hammond, Erin E. Mcclure Carroll, Dana K. Shaw, Brenden G. Tully, Amanda D. Buskirk, Shelby L. Ford, L. Rainer Butler, Preeti Shahi, Kateryna Morozova, Cristina C. Clement, Lauren Lawres, Anya J. O'Neal, Choukri Ben Mamoun, Kathleen L. Mason, Brandi E. Hobbs, Glen A. Scoles, Eileen M. Barry, Daniel E. Sonenshine, Utpal Pal, Jesus G. Valenzuela, Marcelo B. Sztein, Marcela F. Pasetti, Michael L. Levin, Michail Kotsyfakis, Steven M. Jay, Jason F. Huntley, Lloyd S. Miller, Laura Santambrogio, Joao H.F. Pedra

Biological Sciences Faculty Publications

Extracellular vesicles are thought to facilitate pathogen transmission from arthropods to humans and other animals. Here, we reveal that pathogen spreading from arthropods to the mammalian host is multifaceted. Extracellular vesicles from Ixodes scapularis enable tick feeding and promote infection of the mildly virulent rickettsial agent Anaplasma phagocytophilum through the SNARE proteins Vamp33 and Synaptobrevin 2 and dendritic epidermal T cells. However, extracellular vesicles from the tick Dermacentor andersoni mitigate microbial spreading caused by the lethal pathogen Francisella tularensis. Collectively, we establish that tick extracellular vesicles foster distinct outcomes of bacterial infection and assist in vector feeding by acting …

Global Extinction Risk For Seahorses, Pipefishes And Their Near Relatives (Syngnathiformes), Riley A. Pollom, Gina M. Ralph, Caroline M. Pollock, Amanda C.J. Vincent

Biological Sciences Faculty Publications

Few marine taxa have been comprehensively assessed for their conservation status, despite heavy pressures from fishing, habitat degradation and climate change. Here we report on the first global assessment of extinction risk for 300 species of syngnathiform fishes known as of 2017, using the IUCN Red List criteria. This order of bony teleosts is dominated by seahorses, pipefishes and seadragons (family Syngnathidae). It also includes trumpetfishes (Aulostomidae), shrimpfishes (Centriscidae), cornetfishes (Fistulariidae) and ghost pipefishes (Solenostomidae). At least 6% are threatened, but data suggest a mid-point estimate of 7.9% and an upper bound of 38%. Most of the threatened species are …

Diet Of Long-Eared Owl And Common Kestrel In An Urban Landscape (Ukraine), Ivan Zahorodnyi, Oleksii Dubovyk, Ivan Komarnytskyi, Ihor Dykyy

Biological Sciences Faculty Publications

In the present study we performed a comparative dietary analysis of two predatory birds, the Long-eared Owl (Asio otus) and the Common Kestrel (Falco tinnunculus) in the district of Lviv city. We found that the Long-eared Owl and the Common Kestrel are typical small mammal specialists within the urban ecosystem. Considering the abundance and biomass of prey, small mammals comprise 98.4% of the Long-eared Owl’s diet. The species composition of mammals coincides almost 50% in the food intake comparison of the two birds. It has been established that the main prey of both species is the …

Consensus Guidelines For Advancing Coral Holobiont Genome And Specimen Voucher Deposition, Christian R. Voolstra, Kate M. Quigley, Sarah W. Davies, John Everett Parkinson, Raquel S. Peixoto, Manuel Aranda, Andrew C. Baker, Adam R. Barno, Daniel J. Barshis, Francesca Benzoni, Victor Bonito, David G. Bourne, Carol Buitrago-López, Tom C.L. Bridge, Cheong Xin Chan, David J. Combosch, Jamie Craggs, Jörg C. Frommlet, Santiago Herrera, Andrea M. Quattrini, Till Röthig, James D. Reimer, Esther Rubio-Portillo, David J. Suggett, Helena Villela, Maren Ziegler, Michael Sweet

Biological Sciences Faculty Publications

Coral research is being ushered into the genomic era. To fully capitalize on the potential discoveries from this genomic revolution, the rapidly increasing number of high-quality genomes requires effective pairing with rigorous taxonomic characterizations of specimens and the contextualization of their ecological relevance. However, to date there is no formal framework that genomicists, taxonomists, and coral scientists can collectively use to systematically acquire and link these data. Spurred by the recently announced “Coral symbiosis sensitivity to environmental change hub” under the “Aquatic Symbiosis Genomics Project” - a collaboration between the Wellcome Sanger Institute and the Gordon and Betty Moore Foundation …