Digital Commons Network^™

Characterization Of The Cazy Repertoire From The Marine-Derived Fungus Stemphylium Lucomagnoense In Relation To Saline Conditions, Wissal Ben Ali, David Navarro, Abhishek Kumar, Elodie Drula

Open Access archive

Even if the ocean represents a large part of Earth’s surface, only a few studies describe marine-derived fungi compared to their terrestrial homologues. In this ecosystem, marine-derived fungi have had to adapt to the salinity and to the plant biomass composition. This articles studies the growth of five marine isolates and the tuning of lignocellulolytic activities under different conditions, including the salinity. A de novo transcriptome sequencing and assembly were used in combination with a proteomic approach to characterize the Carbohydrate Active Enzymes (CAZy) repertoire of one of these strains. Following these approaches, Stemphylium lucomagnoense was selected for its adapted …

Microglial Response To Aspergillus Flavus And Candida Albicans: Implications In Endophthalmitis, Jaishree Gandhi, Poonam Naik, Inderjeet Kaur, Ashok Kumar

Open Access archive

Aspergillus flavus is the most common etiology of fungal endophthalmitis in India, while Candida albicans is the causative agent in the West. In this study, we determined the role of microglial cells in evoking an inflammatory response following an infection with A. flavus and C. albicans strains isolated from patients with endophthalmitis. Microglia (CHME-3) cells were infected with A. flavus and C. albicans and the expression of Toll-Like Receptors (TLRs), cytokines and Matrix metalloproteinases (MMPs) were assessed at various time intervals. A. flavus infected cells induced higher expressions of TLR-1,-2,-5,-6,-7 and-9 and cytokines such as IL-1α, IL-6, IL-8, IL-10 and …

Recognition And Repetition Counting For Local Muscular Endurance Exercises In Exercise-Based Rehabilitation: A Comparative Study Using Artificial Intelligence Models, Ghanashyama Prabhu, Noel E. O’Connor, Kieran Moran

Open Access archive

Exercise-based cardiac rehabilitation requires patients to perform a set of certain prescribed exercises a specific number of times. Local muscular endurance exercises are an important part of the rehabilitation program. Automatic exercise recognition and repetition counting, from wearable sensor data, is an important technology to enable patients to perform exercises independently in remote settings, e.g., their own home. In this paper, we first report on a comparison of traditional approaches to exercise recognition and repetition counting (supervised ML and peak detection) with Convolutional Neural Networks (CNNs). We investigated CNN models based on the AlexNet architecture and found that the performance …

Genetic And Epigenetic Stability Of Stem Cells: Epigenetic Modifiers Modulate The Fate Of Mesenchymal Stem Cells, Shikha Sharma, Ramesh Bhonde

Open Access archive

Stem cell research has progressed widely and has been receiving a considerable attention for its advantages and drawbacks. Despite their extensive therapeutic potential in regenerative medicine, they are debatable for their genetic and epigenetic stability. In fact lineage specific differentiation is mediated via epigenetic changes in DNA methylation, acetylation, histone modifications etc. Thus epigenetics plays an important role in stem cell biology. For therapeutic interventions stem cells need to be genetically and epigenetically stable for their maximum paracrine secretions for bringing about expected tissue repair and regeneration. In this review we have focused on the current status of genetic and …

Gardner-Webb Announces New Vice President For University Advancement, Office Of University Communications

Gardner-Webb NewsCenter Archive

Officials at Gardner-Webb University have announced the selection of a new member of senior staff to lead the Advancement team. Nathaniel “Nate” J. Evans, of Greenville, N.C., will begin his role as Vice President of Advancement on October 5, 2020, and will direct the offices of alumni relations, major gifts, the Bulldog Club, and the annual fund. Most recently, Evans served as senior advancement officer at East Carolina University (ECU) in Greenville, N.C.

Colorado, Richard Arnett, Emeka Ezekwemba, Micah Hardy

Water Law Review

No abstract provided.

Taxonomic Revision Of Agraphydrus Régimbart , 1903 V. Philippine Species And Their First Dna Barcodes (Coleoptera: Hydrophilidae: Acidocerinae), Hendrik Freitag, Albrecht Komarek

Biology Faculty Publications

The genus Agraphydrus RÉGIMBART , 1903 (Coleoptera: Hydrophilidae) of the Republic of the Philippines is taxonomically reviewed aided by COI mtDNA barcodes. Nine new species are described, all currently regarded as endemic to the Philippines: A. abrasus sp.n., A. ampullatus sp.n., A. batak sp.n., A. brevilobatus sp.n., A. occultus sp.n., A. palawanensis sp.n., A. pelingeni sp.n., A. tenuipalpis sp.n., and A. zetteli sp.n. Agraphydrus coomani (O RCHYMONT , 1927), widespread in the Oriental and Australasian regions, is recorded from the Philippines for the first time. COI sequences and thorough morphological examination suggest that this and further species represent a group …

Analyses Of The Expression, Immunohistochemical Properties And Serodiagnostic Potential Of Schistosoma Japonicum Peroxiredoxin-4, Minh-Anh Dang-Trinh, Jose Ma. M. Angeles, Kharleezelle J. Moendeg, Adrian Miki C. Macalanda, Thu-Thuy Nguyen, Luna Higuchi, Shotaro Nakagun, Masahi Kirinoki, Yuichi Chigusa, Yasuyuki Goto, Shin-Ichiro Kawazu

Biology Faculty Publications

Background

Schistosoma japonicum, which inhabits the mesenteric vein of the mammalian hosts for about 20 to 30 years, is subjected to the oxidative stresses from the host defense mechanism during their intra-mammalian stages. To counteract this host immune attack, the parasite utilizes their antioxidant system for survival inside the host. Peroxiredoxins (Prxs), thiol-specific antioxidant proteins, play an essential role for protecting the parasite against oxidative stress by reducing hydrogen peroxide to water. Only three types of 2-Cys Prxs have been previously characterized in S. japonicum whereas a fourth Prx has been identified for Schistosoma mansoni as Prx-4. A sequence …

Hss 404-H01: Human Subjects - The History Of Experimenting On Humans, Rosanna Dent

History Syllabi

No abstract provided.

September 2020 News Releases, University Of Montana--Missoula. Office Of University Relations

University of Montana News Releases, 1928, 1956-present

No abstract provided.

Forage News [2020-09], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

No abstract provided.

A Thing Called Character: Bringing The Export-Import Bank Of The United States And West Virginia To The World, Kimberly A. Reed

West Virginia Law Review

No abstract provided.

Efficacy And Safety Of First-Line Avelumab In Patients With Advanced Non-Small Cell Lung Cancer: Results From A Phase Ib Cohort Of The Javelin Solid Tumor Study., Claire F Verschraegen, Guy Jerusalem, Edward F Mcclay, Nicholas Iannotti, Charles H Redfern, Jaafar Bennouna, Franklin L Chen, Karen Kelly, Janice Mehnert, John C Morris, Matthew H Taylor, David Spigel, Ding Wang, Hans Juergen Grote, Dongli Zhou, Neru Munshi, Marcis Bajars, James L Gulley

Articles, Abstracts, and Reports

INTRODUCTION: Avelumab, an antiprogrammed death ligand-1 antibody, is approved as a monotherapy for treatment of metastatic Merkel cell carcinoma and advanced urothelial carcinoma, and in combination with axitinib for advanced renal cell carcinoma. We report the efficacy and safety of first-line avelumab in advanced non-small cell lung cancer (NSCLC).

METHODS: In a phase I expansion cohort of the JAVELIN Solid Tumor trial, patients with treatment-naive, metastatic, or recurrent NSCLC received 10 mg/kg avelumab intravenously every 2 weeks. Endpoints included best overall response, duration of response (DOR), progression-free survival (PFS), overall survival (OS), and safety.

RESULTS: Overall, 156 patients were enrolled …

Defining The Proteolytic Landscape During Enterovirus Infection., Mohsan Saeed, Sebastian Kapell, Nicholas T Hertz, Xianfang Wu, Kierstin Bell, Alison W Ashbrook, Milica Tesic Mark, Henry A Zebroski, Maxwell L Neal, Malin Flodström-Tullberg, Margaret R Macdonald, John D Aitchison, Henrik Molina, Charles M Rice

Articles, Abstracts, and Reports

Viruses cleave cellular proteins to remodel the host proteome. The study of these cleavages has revealed mechanisms of immune evasion, resource exploitation, and pathogenesis. However, the full extent of virus-induced proteolysis in infected cells is unknown, mainly because until recently the technology for a global view of proteolysis within cells was lacking. Here, we report the first comprehensive catalog of proteins cleaved upon enterovirus infection and identify the sites within proteins where the cleavages occur. We employed multiple strategies to confirm protein cleavages and assigned them to one of the two enteroviral proteases. Detailed characterization of one substrate, LSM14A, a …

Trident - Fall 2020, Dugoni School Of Dentistry

Trident

The newsletter of the Pacific Orthodontic Alumni Chapter at University of the Pacific, Arthur A. Dugoni School of Dentistry.

Unh: The Magazine Of The University Of New Hampshire, Fall 2020, Unh Magazine

UNH Magazine

This editon of UNH Magazine was digital only due to the COVID-19 Pandemic. This is an archived version of web content originally found at https://magazine.unh.edu/

Ubc-9 Acts In Gaba Neurons To Control Neuromuscular Signaling In C. Elegans, Victoria A. Kreyden, Elly B. Mawi, Jennifer Kowalski

Scholarship and Professional Work - LAS

Regulation of excitatory to inhibitory signaling balance is essential to nervous system health and is maintained by numerous enzyme systems that modulate the activity, localization, and abundance of synaptic proteins. SUMOylation is a key post-translational regulator of protein function in diverse cells, including neurons. There, its role in regulating synaptic transmission through pre- and postsynaptic effects has been shown primarily at glutamatergic central nervous system synapses, where the sole SUMO-conjugating enzyme Ubc9 is a critical player. However, whether Ubc9 functions globally at other synapses, including inhibitory synapses, has not been explored. Here, we investigated the role of UBC-9 and the …

Cooperation In The International System: An Interdisciplinary Investigation At The Intersection Of International Relations And International Law, Kalyani Unkule

Maurer Theses and Dissertations

A conversation between the disciplines of International Relations and International Law illuminates the nature of interstate cooperation and enhances our understanding of the nature and potential of international law. There are methodological and practical asymmetries between International Relations and International Law which create ideal conditions for interdisciplinary work. Studying international cooperation on protecting cultural heritage enable us to address the above questions and reevaluate and extend underlying theoretical frameworks.

Quantifying Long-Term Health And Economic Outcomes For Survivors Of Group B Streptococcus Invasive Disease In Infancy: Protocol Of A Multi-Country Study In Argentina, India, Kenya, Mozambique And South Africa, Proma Paul, Simon R. Procter, Ziyaad Dangor, Quique Bassat, Amina Abubakar, Sridhar Santhanam, Romina Libster, Bronner P. Gonçalves, Shabir A. Madhi, Azucena Bardají

Institute for Human Development, East Africa

Sepsis and meningitis due to invasive group B Streptococcus (iGBS) disease during early infancy is a leading cause of child mortality. Recent systematic estimates of the worldwide burden of GBS suggested that there are 319,000 cases of infant iGBS disease each year, and an estimated 147,000 stillbirths and young-infant deaths, with the highest burden occurring in Sub-Saharan Africa. The following priority data gaps were highlighted: (1) long-term outcome data after infant iGBS, including mild disability, to calculate quality-adjusted life years (QALYs) or disability-adjusted life years (DALYs) and (2) economic burden for iGBS survivors and their families. Geographic data gaps were …

Γ9Δ2t Cell Diversity And The Receptor Interface With Tumor Cells, Anna Vyborova, Dennis X. Beringer, Domenico Fasci, Froso Karaiskaki, Eline Van Diest, Lovro Kramer, Aram De Haas, Jasper Sanders, Anke Janssen, Trudy Straetemans, Daniel Olive, Jeanette Leusen, Lola Boutin, Steven Nedellec, Samantha L. Schwartz, Michael J. Wester, Keith A. Lidke, Emmanuel Scotet, Diane S. Lidke, Albert Jr Heck, Zsolt Sebestyen, Jürgen Kuball

Pathology Research and Scholarship

γ9δ2T cells play a major role in cancer immune surveillance, yet the clinical translation of their in vitro promise remains challenging. To address limitations of previous clinical attempts using expanded γ9δ2T cells, we explored the clonal diversity of γ9δ2T cell repertoires and characterized their target. We demonstrated that only a fraction of expanded γ9δ2T cells was active against cancer cells and that activity of the parental clone, or functional avidity of selected γ9δ2 T cell receptors (γ9δ2TCRs), was not associated with clonal frequency. Furthermore, we analyzed the target-receptor interface and provided a 2-receptor, 3-ligand model. We found that activation was …

A Novel Flow Cytometric Assay To Identify Inhibitors Of Rbpj-Dna Interactions, Robert J. Lake, Mark K. Haynes, Kostiantyn Dreval, Rabeya Bilkis, Larry A. Sklar, Hua-Ying Fan

Pathology Research and Scholarship

Notch signaling is often involved in cancer cell initiation and proliferation. Aberrant Notch activation underlies more than 50% of T-cell acute lymphoblastic leukemia (T-ALL); accordingly, chemicals disrupting Notch signaling are of potential to treat Notch-dependent cancer. Here, we developed a flow cytometry-based high-throughput assay to identify compounds that disrupt the interactions of DNA and RBPJ, the major downstream effector of Notch signaling. From 1492 compounds, we identified 18 compounds that disrupt RBPJ-DNA interactions in a dose-dependent manner. Cell-based assays further revealed that auranofin downregulates Notch-dependent transcription and decreases RBPJ-chromatin interactions in cells. Most strikingly, T-ALL cells that depend on Notch …

Minimizing Surgical Blood Loss At Cesarean Hysterectomy For Placenta Previa With Evidence Of Placenta Increta Or Placenta Percreta: The State Of Play In 2020, John C. Kingdom, Sebastian R. Hobson, Ally Murji, Lisa Allen, Rory C. Windrim, Evelyn Lockhart, Sally L. Collins, Hooman Soleymani Majd, Moiad Alazzam, Feras Naaisa, Alireza A. Shamshirsaz, Michael A. Belfort, Karin A. Fox

Pathology Research and Scholarship

The evolution of multidisciplinary team-based care for women with placenta accreta spectrum disorder has delivered stepwise improvements in clinical outcomes. Central to this overall goal is the ability to limit blood loss at surgery. Placement of inflatable balloons within the pelvic arteries, most commonly in the anterior divisions of the internal iliac arteries, became popular in many centers, at the expense of prolonging surgical care and with attendant risks of vascular injury. In tandem, the need to expose pelvic sidewall anatomy to safely identify the course of the ureters re-popularized the alternative strategy of ligating the same anterior divisions of …

Postmortem Computed Tomography In Firearm Homicides: A Retrospective Case Series, Marloes E M Vester, Kurt B Nolte, Gary M. Hatch, Chandra Y. Gerrard, Reinoud D. Stoel, Rick R. Van Rijn

Pathology Research and Scholarship

Postmortem computed tomography (PMCT) is integrated into the evaluation of decedents in several American medical examiner offices and medicolegal death investigative centers in many other countries. We retrospectively investigated the value of PMCT in a series of firearm homicide cases from a statewide centralized medical examiner's office that occurred during 2016. Autopsies were performed or supervised by board-certified forensic pathologists who reviewed the PMCT scans prior to autopsy. PMCT scans were re-evaluated by a forensic radiologist blinded to the autopsy findings and scored by body region (head-neck, thoracoabdominal, and extremities). Injury discrepancies were scored using a modified Goldman classification and …

A Delicate Balance: Limiting Consolidation In Agricultural Seed Markets Without Stifling Innovation, Justin Brickey

The Business, Entrepreneurship & Tax Law Review

Agricultural seed markets have experienced significant consolidation over the past fifty years, apparently driven by private investment in research and development (“R&D”) made profitable through extensive intellectual property protections. The current intellectual property law regime on plants allows an innovator to obtain a wide variety of protections on new plant varieties, whether created through natural breeding processes or genetic engineering. Investment has paid off and the United States has experienced significant benefits through increased productivity, meaning we produce significantly more food today without using more land than our grandparents. However, agricultural markets deserve, and are often given, unique consideration because …

Summer Field Report, June-July 2020, W. Ross Silcock

Nebraska Bird Review

Overall, this was a fairly routine summer although birders in Nebraska know that “routine” still holds promise of a few happenings out of the ordinary. The summer season primarily examines new breeding records, range expansions, and changes in numbers, up or down. Mississippi Kites were conspicuous, a pair providing Scottsbluff’s first breeding record, but a repeating pair in Lincoln, Lancaster lost its nest in a windstorm. The first nesting record for Violet-Green Swallow for Sheridan, 7th nesting for White-winged Dove, and 9th for Short-eared Owl were all encouraging, as was an apparent family group of Northern Saw-whet Owls in Sioux. …

Robert Wolcott And The Art Of Nest Records, Thomas E. Labedz

Nebraska Bird Review

The autumn of 1894 was fortuitous for Nebraska ornithology because it is when Robert Henry Wolcott accepted an assistantship at the University of Nebraska, moving to Lincoln from the University of Michigan (Swenk 1935). Wolcott, from that point onward, made significant contributions to our knowledge of Nebraska birds (Bruner et al. 1903, Wolcott 1909, 1919), was a founding member of the Nebraska Ornithologists’ Union (NOU), and was editor for the first three Proceedings of the NOU from 1899-1902. His work expanded beyond local focus and he eventually authored a text on animal biology (Wolcott 1933). The topic of this paper, …

The Nebraska Bird Review, Volume 88 September 2020, Number 3

Nebraska Bird Review

Summer Field Report, June - July 2020, by W. Ross Silcock …94.

Robert Wolcott and the Art of Nest Records, by Thomas E. Labedz …112

2019 (31st) Report of the NOU Records Committee, by Mark A. Brogie …124

Subscription and Organization Information …135

Racism In Singapore, Kayne Augustin, Matthew Davaraj Danapaul, Muhammad Irfan Bin Arshad, Muhammad Fakhrudin, Jun Jie Jeremy Teng

Introduction to Research Methods RSCH 202

Racism is a prevalent issue around the world. In Singapore, concerns of discrimination against minority racial groups, such as Malays and Indians, have been raised with regards to companies’ employing procedures. However, there have been very little studies done on this issue. We have included relevant studies that support and do not support the claim of presence of racism in Singapore. In addition, we intend to replicate a previous study conducted by Marianne Bertrand and Sendhil Mullainathan (2004) in America by sending fabricated resumes to white-collared industries, such as the law, finance, and real estate firms in Singapore. These firms …

Issues Of Theory And Method In The Analysis Of Paleolithic Mortuary Behavior: A View From Shanidar Cave, Emma Pomeroy, Chris O. Hunt, Tim Reynolds, Dlshad Abdulmutalb, Eleni Asouti, Paul Bennett, Marjolein Bosch, Ariane Burke, Lucy Farr, Robert Foley, Charles French, Amos Frumkin, Paul Goldberg, Evan Hill, Ceren Kabukcu, Marta Mirazón Lahr, Ross Lane, Curtis Marean, Bruno Maureille, Giuseppina Mutri, Christopher E. Miller, Kaify Ali Mustafa, Andreas Nymark, Paul Pettitt, Nohemi Sala, Dennis Sandgathe, Chris Stringer, Emily Tilby, Graeme Barker

KIP Articles

Mortuary behavior (activities concerning dead conspecifics) is one of many traits that were previously widely considered to have been uniquely human, but on which perspectives have changed markedly in recent years. Theoretical approaches to hominin mortuary activity and its evolution have undergone major revision, and advances in diverse archeological and paleoanthropological methods have brought new ways of identifying behaviors such as intentional burial. Despite these advances, debates concerning the nature of hominin mortuary activity, particularly among the Neanderthals, rely heavily on the rereading of old excavations as new finds are relatively rare, limiting the extent to which such debates can …

Applying The Values Of Geoethics For Sustainable Speleotourism Development, Aleksandar Antić, Silvia Peppoloni, Giuseppe Di Capua

KIP Articles

Establishing sustainable and responsible speleotourism development is a major challenge and involves complex activities. Adequate theoretical starting point is the application of geoethical values related to the conservation and protection of the caves to be used for touristic purposes. Positive and negative cases of human behaviors towards speleological geoheritage are discussed, in order to highlight what should be done in cave management to avoid malpractices and on what elements could be founded adequate strategies aimed at promoting sustainable speleotourism. This is important to tourism management organizations involved in the promotion of caves and in creating economic opportunities for local populations, …