Digital Commons Network^™

Biological And Computational Studies Of The Structure And Function Of Pul103, A Human Cytomegalovirus Tegument Protein, Ashley N. Anderson

Wayne State University Dissertations

Human cytomegalovirus (HCMV) is an enveloped, single segment, double-stranded DNA virus. HCMV infection causes disease in immunocompromised (HIV patients, transplant recipients) and immunodeficient (fetuses, neonates) populations. Current treatments are effective but are either limited in use or can lead to organ damage and/or antiviral resistance, and no vaccines are available. Additional antiviral targets are needed. HCMV pUL103 is a potential antiviral target. pUL103 is a conserved herpesvirus protein present in the tegument, layer of proteins and RNA between the envelope and capsid of HCMV virions. pUL103 helps reorganize cellular secretory machinery (Golgi, endosomes) to form the cytoplasmic virion assembly compartment …

Novel Insights Into The Critical Role Of Cardiolipin In Cellular Metabolism And Mitochondrial Physiology, Jiajia Ji

Wayne State University Dissertations

Cardiolipin (CL) is the signature phospholipid of mitochondria. CL and its remodeling exert critical roles in biological processes both inside and outside of mitochondria. CL abnormalities have been associated with various mitochondrial disorders and aging. Understanding the role of CL in mitochondrial physiology and cellular metabolism could provide valuable insights into cell biology and human health. Several metabolic alterations have been reported in CL-deficient cells, including accumulated lactate, decreased PDH activity, and decreased TCA cycle function. This dissertation connected these findings by showing abnormal NAD+ metabolism in various models lacking CL. Importantly, it shows that NAD+ supplementation improves mitochondrial function …

Towards Personalized Medicine: Computational Approaches For Drug Repurposing And Cell Type Identification, Azam Peyvandipour

Wayne State University Dissertations

The traditional drug discovery process is extremely slow and costly. More than 90% of drugs fail to pass beyond the early stage of development and toxicity tests, and many of the drugs that go through early phases of the clinical trials fail because of adverse reactions, side effects, or lack of efficiency. In spite of unprecedented investments in research and development (R&D), the number of new FDA-approved drugs remains low, reflecting the limitations of the current R&D model.

In this context, finding new disease indications for existing drugs sidesteps these issues and can therefore increase the available therapeutic choices at …

A Mechanism For Sex Determination In Dioecious Cultivated Spinach, Nicholas West

Wayne State University Dissertations

While unisexual flowers have evolved repeatedly throughout angiosperm families, the actual identity of sex determining genes has been elusive, and their regulation within populations remains largely undefined. Additionally, sex liability is often observed in unisexual plants and has been correlated to external and internal cues, suggesting that the genes responsible for unisexual morphology are not necessarily segregating but rather differentially regulated. Understanding these processes will be of significant theoretical and agronomical importance.

Cultivated spinach is a dioecious species in which an individual will bear alternative sexual organs. Previous work has identified spinach B class floral organ identity genes, SpAP3 and …

Germinal Center B Cell Expression Of Aire Regulates Antibody Diversification And Autoimmunity, Jordan Zheng Zhou

Wayne State University Dissertations

B cells are a unique subset of immune cells that, in response to antigen, diversify their antibody repertoire to generate progressively higher affinity antibodies of different isotypes through the processes of affinity maturation/somatic hypermutation (SHM) and class switch recombination (CSR). One of the major sites in which this diversification occurs is in T cell dependent microanatomical structures known as germinal centers (GC). Here, we find that GC B cells express the protein, autoimmune regulator (Aire) in a CD40 dependent manner. In these cells, Aire interacts with activation induced cytidine deaminase (AID), the protein responsible for initiating the processes of diversification …

Innovation In Meat Production: A Problem And An Opportunity, Christopher J. Bryant

Animal Sentience

Innovation in meat production has enabled modern humans to inflict far greater harm on animals, the environment, and public health than was possible just a few decades ago. Wiebers & Feigin aptly express the urgency with which these issues must be addressed. Those advocating for animals on moral grounds face resistance from omnivores citing taste, price and convenience. Further innovation in meat production (plant-based and cultured meat) will enable us to preserve the experience of eating meat whilst phasing out the many problems caused by industrial animal farming.

Re-Engage With The World For Global Health And Animal Welfare, Bradley J. Bergstrom

Animal Sentience

Wiebers & Feigin (2020) make a strong argument for measures that would limit future zoonoses, such as the COVID-19 pandemic, by closing live-animal markets, changing our habits of food consumption and production, and reducing habitat destruction. These would help human health, animal welfare, and conservation of at-risk wildlife all at the same time. China’s command-and-control government can accomplish some of these things by edict, but slower-to-act western democracies play a surprisingly large role in these global problems by the power of their consumerism, including the illicit wildlife trade. We citizens need to insist that our government use all of its …

Thinking Longer, Looking Deeper, Ronnie Z. Hawkins

Animal Sentience

We need to situate the present crisis within the larger context of what we humans have done to the nonhuman forms of life with which we evolved, taking a longer view of our own evolutionary origins and a deeper look at what might be a more appropriate role for our species to play within the Biosphere.

Mental Illness Awareness In The State Of New Hampshire, Susan Pomerleau

Selected Social Change Portfolios in Prevention, Intervention, and Consultation

New Hampshire is a small state with 1.36 million people reported in 2019. In New Hampshire, 4% of adults live with serious mental health conditions, such as schizophrenia, bipolar disorder, and major depression (Resources to Recover, 2020). The National Alliance of Mental Illness (NAMI) has a chapter in New Hampshire to help support, promote, and educate on the topic of mental illness. I would like to partner my effort with NAMI NH and also with the STAMP OUT STIGMA Campaign through the Association for Behavioral Health and Wellness (ABHW) to bring greater awareness of serious mental illness (SMI) and any …

Minority Women Experiencing Postpartum Depression In Colorado, Heather Schunke

Selected Social Change Portfolios in Prevention, Intervention, and Consultation

One of the most common complications associated with childbirth is pregnancy-related depression. Postpartum Depression (PPD) affects a new mother’s ability to bond with and take care of their baby. When left untreated PPD not only impacts the mother’s health but can also cause behavioral, eating, and sleeping issues for the baby (CDC, 2020). New mothers experiencing PPD have reported feeling stressed, worried, and anxious about their children. New mothers also reported having doubts about their ability to be a good mother. Almost half of the new mothers experiencing PPD will not seek treatment. Minority women are even less likely to …

Cpet Is The Phycoerythrobilin Lyase For Cys-165 On Beta-Phycoerythrin From Fremyella Diplosiphon And The Chaperone-Like Protein Cpez Greatly Improves Its Activity., Wendy M. Schluchter, A. A. Nguyen, K. L. Joseph, A. N. Bussell, S. Pokhrel, A. J. Karty, M. C. Kronfel, D. M. Kehoe

Biological Sciences Faculty Publications

Bilin lyases are enzymes which ligate linear tetrapyrrole chromophores to specific cysteine residues on light harvesting proteins present in cyanobacteria and red algae. The lyases responsible for chromophorylating the light harvesting protein phycoerythrin (PE) have not been fully characterized. In this study, we explore the role of CpeT, a putative bilin lyase, in the biosynthesis of PE in the cyanobacterium Fremyella diplosiphon. Recombinant protein studies show that CpeT alone can bind phycoerythrobilin (PEB), but CpeZ, a chaperone-like protein, is needed in order to correctly and efficiently attach PEB to the beta-subunit of PE. MS analyses of the recombinant beta-subunit of …

Triflic Acid Mediated Sequential Cyclization Of Ortho-Alkynylarylesters With Ammonium Acetate, Maciej E. Domaradzki, Xiaochen Liu, Jiye Ong, Gyeongah Yu, Gan Zhang, Ariel Simantov, Eliyahu Perl, Yu Chen

Publications and Research

A triflic acid (TfOH) mediated sequential cyclization of ortho-alkynylarylesters and ammonium acetate (NH₄OAc) was reported. The reaction took place via a Brønsted acid-mediated intramolecular cyclization of ortho-alkynylarylesters followed by an ammonium acetate participated substitution reaction, forming isoquinolin-1-ones as the major products. Different from most of the known synthetic methods of isoquinolin-1-ones, no metal catalyst was required in the reported reaction. The regioisomers – isoindolin-1-ones were obtained together with isoquinolin-1-ones in a few cases. The intermediate compounds – isochromen-1-ones and isobenzofuran-1-ones were also isolated. The interconversion experiments showed that the regioisomers formed during the Brønsted acid induced …

Barrels Xxxii Meeting Report: Whiskers In The Windy City, Giuseppe Cataldo, Chia-Chien Chen, Alicia C. Barrientos, Joshua C. Brumberg

Publications and Research

The 32^nd Annual Barrels meeting was hosted at the Northwestern University Feinberg School of Medicine in Chicago, Illinois on October 17^th and 18^th, 2019. The annual meeting brings together researchers who utilize the rodent whisker-to-barrel system as a means to understand cortical function and development. This year’s meeting focused on social behaviors, development and cerebellar functions within the barrel system and beyond.

Learning And Motivation For Rewards In Schizophrenia: Implications For Behavioral Rehabilitation, Victoria Martin, Alexandra Brereton, Jicheng Tang

Publications and Research

Purpose of review: Impaired reward processing and amotivation are well documented in schizophrenia. We aim to review the current state of neuroimaging and behavioral research addressing components of motivational deficits in this complex and impairing syndrome. Evidence will be integrated to inform the ongoing development of effective strategies for behavioral rehabilitation. Recent findings: While striatal dopamine and aberrant reward prediction errors have been connected to amotivation in schizophrenia, they are not sufficiently full explanations of reward processing impairments. Frontal dysfunction and associated cognitive control deficits also have evidenced involvement in atypical reward prediction, learning, and valuation. Ongoing work supports the …

Keynesian Uncertainty: The Great Divide Between Joan Robinson And Paul Samuelson In Their Correspondence And Public Exchanges, Harvey Gram, G. C. Harcourt

Publications and Research

Joan Robinson and Paul Samuelson found little to agree upon in a correspondence which began in 1946, shortly after the death of Keynes, and ended a year prior to Robinson’s death in 1983. One way to read the correspondence is to keep in mind that Keynesian uncertainty was central to Robinson’s understanding of how capitalist economies function. Samuelson, never impressed by Keynes’s handling of uncertainty, understood capital theory—if not capitalism—in terms of dynamic programming, with its perfect foresight entailments. This is evident throughout his letters to Robinson, although rarely acknowledged in a straightforward way, particularly during the period from 1971 …

A National Study Of Internship Directors' Perspectives On Psychological Assessment Practices, Elizabeth Shipley

Theses and Dissertations

This study examined the perspectives of a national sample of pre-doctoral internship directors regarding psychological testing and assessment practices at the internship level. Study participants were from member programs of the Association of Psychology Postdoctoral and Internship Centers (APPIC). The study's goals were to better understand the current state of affairs regarding the role of assessment in internship programs and to develop findings that may be useful to academic training programs and graduate students in psychology. A 32-item questionnaire was developed which inquired about general internship program information, types of assessment instruments used at each internship setting, the form and …

Best Teaching Practices To Increase Student Interest In Stem Subjects, Talar Gullapyan

Theses and Dissertations

More than ever, jobs at all levels, not just for scientists, are requiring STEM knowledge and specific abilities that are associated with a STEM education (Lacey & Wright, 2009; Rothwell, 2013). Additionally, The Bureau of Labor and Statistics (Vilorio, 2014) reports that STEM occupations will continue to grow faster than other occupations. While the demand for jobs in STEM fields continues to grow, many of these positions remained unfilled due to an unskilled labor force. Students in the U.S. also pursue far fewer degrees in STEM subjects compared to other competitive countries (National Science Board, 2012). The results from the …

#Livingwhileblack: A Mixed-Method Exploratory Analysis Of The Social Media Documentation Of Racism Via Twitter And Interviews, Judy A. Jackson

Theses and Dissertations

African Americans have endured marginalization related to racial and cultural disparities. #LivingWhileBlack has become a unique repository of all related experiences that Black people are encountering, while performing every day, seemingly mundane tasks and activities. The #LivingWhileBlack hashtag went viral on Twitter (https://twitter.com/), allowing users, including Black Twitter (https://twitter.com/), to use the international platform to share their own Black story and experience. The three research questions that were explored included the following. First: How are profiling events related to #LivingWhileBlack events documented, reported and expressed by Social Media Users? Second: How are others/people using Twitter (https://twitter.com/); affected by various incidences …

Resource For A Brief Early Somatic Intervention To Reduce Symptoms Of Post-Traumatic Stress For Victims Of Violence Crime In Acute Hospital Settings In Southeast Los Angeles, Jamie Gamboa

Theses and Dissertations

The goal of this dissertation was to create an intervention resource guide containing recommendations that can be utilized by the South Los Angeles Trauma Recovery Center (SLATRC) to implement a brief early somatic intervention for victims of violent crime in acute hospital settings in Southeast Los Angeles. It was decided that an intervention based upon the Trauma Resiliency Model/Community Resiliency Model (TRM/CRM) would best fit the needs of the population. First, a systematic literature review was conducted to increase the efficacy of the resource by gathering information on similar interventions. The specific questions guiding this review were as follows: (1) …

Development Of A Resilience-Oriented Stress Management Group Intervention For Mothers Experiencing Homelessness, Lily Danielle Rowland

Theses and Dissertations

Family homelessness, particularly of mothers and their children, continues to remain an unfortunate epidemic throughout the United States. Personal, relational, and systemic adversity and trauma leave this population at high risk for instability, physical and mental health challenges, and impaired wellbeing. However, despite these difficulties, research strongly suggests that positive functioning can remain in families experiencing homelessness and that many go on to leave shelters and maintain stability, particularly when mothers have effective strategies to manage stress in contexts of adversity. In addition to identifying the needs of mothers experiencing homelessness, the literature also has highlighted factors of individual and …

Developing Ei: Leadership Journeys In Emotional Intelligence, Erin Bric

Theses and Dissertations

Emotional intelligence (EI) has been deemed a critical competency for leaders in today's world. However, there is little information to help OD practitioners, leaders, and organizations understand how leaders can develop these skills in ways that meaningfully and positively impact their teams and organizations. This study examined the journeys of senior leaders as they developed their emotional intelligence. The study addressed two research questions: how leaders perceive they have developed their emotional intelligence throughout their lifetimes and how leaders are continuing to develop their emotional intelligence in their lives and careers. Key themes emerged out of leader interviews and were …

Climate Change, Social Media, And Generation Z, Melanie Morris

Theses and Dissertations

Recent publications report that adults known as Generation Z, between the ages of 18 to 23, increasingly rely upon social media to gain knowledge of social issues. Given social media's embeddedness in Generation Z's life, this study sought to understand if or how social media has influenced and possibly empowered Generation Z to act on social issues, particularly global climate change. This study used a phenomenological research method, which focused on the commonality of Generation Z's lived experience. Emerging themes collected through a literature review and data portray Generation Z as maturing into adulthood as tech-savvy, diverse, and inclusive self-starters. …

Leading, Coaching, & Mentoring: A Study Of Coach-Athlete Relationships As Associated Factors In Performance, Katie D. Kamachi

Theses and Dissertations

This research focused on global leadership and change and competitive athletics. Servant leadership, including the five servant leadership variables; altruistic calling, emotional healing, wisdom, persuasive mapping, and organizational stewardship, as well coach athlete relationships, including the three coach-athlete relationship variables; closeness, commitment, and complementarity, were used to analyze athlete perceptions. This study then found correlations of the associated factors to outcome, at team and individual levels. Findings from this study include recognizing the commitment of the head coach as a factor in team mins; individual athlete perception of closeness as related to games started; the emotional healing aspects from the …

Homeless With Breast Cancer On Skid Row, Los Angeles: A Review Of Services For Women, Eva Dittrich

Theses and Dissertations

This study reviews the literature on breast cancer in general and narrows to within the context of the homeless population of Skid Row, Los Angeles, positing that the experience of breast cancer while homeless is a compounded traumatic experience with complex and numerous practical challenges. It poses the following question: What services are available to homeless women on Skid Row, Los Angeles, the information about which can be found online? Which and how many services are dedicated to breast cancer screenings and treatment? Are other types of cancer screening and treatment services advertised on the included service organizations' websites? A …

Advancing Leadership Consciousness: Integrity From The Inside Out, Jessica Plancich Shinners

Theses and Dissertations

A poll conducted by the World Economic Forum (2015) found that 86% of respondents perceive that we are facing a global leadership crisis. At the time of this study, the COVID-19 pandemic has impacted the global ecology in dramatic and widespread ways. In light of this uncertain political, economic, sociological, and ecological climate, humanity is in need of consistent and reliable ways of developing exemplary leaders. As society enters into massive technological advancement, leaders and collaborators are at risk of obsolescence if we do not find innovative ways to harness innate human capacities to advance consciousness and co-evolve with technology. …

Inaccurate And Biased Global Media Coverage Underlies Public Misunderstanding Of Shark Conservation Threats And Solutions, Sarah Joy Bittick

Biology Faculty Works

Sharks are a taxon of significant conservation concern and associated public interest. The scientific community largely supports management policies focusing on sustainable fisheries exploitation of sharks, but many concerned members of the public and some environmental advocates believe that sustainable shark fisheries cannot and do not exist and therefore support total bans on all shark fisheries and/or trade in shark products. The belief that sustainable shark fisheries cannot and do not exist persists despite scientific evidence showing that they can and do, and are important to livelihoods. Additionally, many concerned members of the public are only aware of one threat …

Mississippi Teachers Who Are Nationally Board Certified Or Those With Advanced Degrees: Effects On Student Achievement, Amy Tate Barnett

Electronic Theses and Dissertations

The purpose of this research study was to determine the interaction effects national board certified teachers (NBCTs) and teachers with advanced degrees (ADs); while considering school accountability levels, had on student achievement. This study examined whether significant differences existed in student achievement between the eight identified groups of teachers and how the factors of NBCT status, AD status, and accountability contributed. The study examined student achievement in grades three through eight on the end-of-the-year state assessments for the 2017-18 school year. This research endeavor relied upon the recruitment of local Mississippi school districts and their willingness to participate and share …

Chemical And Biological Evaluations Of Asimina Triloba And Rollinia Mucosa., Taghreed Ali Majrashi

Electronic Theses and Dissertations

Studies have shown the potential risks of neurodegeneration associated with chronic consumption of plants of the Annonaceae family, which emphasize the need for further studies to identify the neurotoxic compounds in the edible annonaceous plants and compare their risk to their benefits in defeating cancer. Our study is focused on the alkaloidal constituents of two plants from the Annonaceae family, Asimina triloba and Rollinia mocusa. This study was carried out to explore the anticancer and neurotoxic constituents of the plants with the intention that separating neurotoxic constituents would help in reducing the undesirable effects of the plants if it is …

Differential Semantics, William Michael Cox

Electronic Theses and Dissertations

Differential Semantics is a theoretical accounting of the semantic complexity found in natural language, particularly that of the academic and literary registers. It addresses natural language semantics in terms of its contribution to the characterization and expression of creative thought, beginning the perception and conceptualization of objective reality, folloby the metacognitive development of idiosemantic connotations in reference to those conceptualizations, and finally, the intuitive process of implication and inference that facilitates the abstraction and communication of thought.

Fabrication Of Taste Masked Tablets Via Fused Deposition Modeling 3d Printing Paired With Hot-Melt Extrusion Techniques, Honghe Wang

Electronic Theses and Dissertations

The objective of this work was to develop taste-masked donut-shaped tablet formulations utilizing fused deposition modeling three-dimensional (3D) printing paired with hot-melt extrusion (HME) techniques. Caffeine citrate (CC) was used as the model drug for its bitter taste, and a three-point bend test was performed to assess the printability of filaments. The stiffness constant was calculated to represent the printability by fitting the breaking distances and stress data into Hooke's law. Formulation F6 and F7 filaments exhibited the desired hardness with a “k” value of 48.30 ± 3.52 g/mm3 and 45.47 ± 3.51 g/mm3, respectively, and were successfully printed. The …