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Neuroanatomy Of The Blackspotted Rockskipper, Entomacrodus Striatus, Pooja Dayal

Williams Honors College, Honors Research Projects

Here I characterized the central neuroanatomy of the Blackspotted Rockskipper, Entomacrodus striatus, native to French Polynesia. The neuroanatomy of E. striatus has not been studied prior to this paper. I used several histology and antibody staining techniques to accomplish this, including Crystal Violet, immunohistochemistry, immunofluorescence, and Bielschowsky’s Silver Nitrate staining. This paper describes the most successful techniques used, identifies major structures in the species’ neuroanatomy, and also explains why studying E. striatus is important in the future of vertebrate research.

The Cardiovascular Response To Acute Hypoxic Conditions In Danio Rerio, Bryce Fetterman

Williams Honors College, Honors Research Projects

There are many fish that can withstand hypoxia levels. One in particular is the zebrafish (Danio rerio). The zebrafish is a small-sized, Cyprinid teleost fish, and has been used in a laboratory setting for many reasons. A few reasons being that they are widely available, easy to care for, and the cost for these fish are very low. There are various reasons why these fish are used as a model. One main reason is because their cardiovascular system is one of the first developing organs. This allows for better resolution when studying the cardiovascular system. The purpose of …

Synthesis, Characterization, And Biological Activity Of Imidazolium Salts, David Weader

Williams Honors College, Honors Research Projects

Nonmuscle invasive bladder cancer (NMIBC) inflicts thousands of Americans annually, and is typically treated with the immunotherapy BCG. However, due to a BCG shortage, there is a new need for novel treatments of NMIBC. Addressing this issue, several imidazolium salt derivatives were synthesized and characterized with the intent of treatment within the bladder. These imidazolium salts were tested against different human bladder cancer cell lines in vitro to determine their reactivity and cytotoxicity. Among these results are GI50 concentrations for each drug, which is the concentration of drug needed to see growth inhibition in 50% of treated cells. Previous published …

Analyzing The Effects Of Opioids On Cortical Pore Networks In Rabbit Tibiae And Femora, Kassidy Wilson

Williams Honors College, Honors Research Projects

The following project was approved by the Institutional Animal Care and Use Committee (IACUC) (Protocol number #18-11-12 ARC). This study was completed as a subset of Dr. Andronowski's overarching lab project. Femora and tibiae were procured from New Zealand White rabbits to study the prolonged effects of opioid use in bone remodeling. This specific project analyzed the effects of opioids on the pore networks and the pore orientation.

Oxidative Stress Analysis Of Placental Tissue In The Rupp Pregnant Rat, Natalie Ganios

Williams Honors College, Honors Research Projects

Preeclampsia is a disease associated with a vasoconstrictive phenotype due to disrupted signaling in the vascular endothelial growth factor (VEGF) relaxation pathway. The purpose of these studies is to determine the level of oxidative damage that is occurring in the placenta of the reduced uterine perfusion pressure (RUPP) rat, which serves as a common model for preeclampsia. RUPP and SHAM placenta tissue will be examined for oxidative stress as an indicator of tissue damage associated with the pathologically induced ischemia. Oxidative stress in the placental tissue will be measured by performing the following oxidative stress assays: 8-isoprostane test, TBARS assay, …

Ecologically Distinct Populations Of Hawaiian Petrel (Pterodroma Sandwichensis): Quantitative Isotopic Analysis To Explore The Uniqueness Of Foraging Habits In Kauai And Hawaii Populations, Drew Casey

Williams Honors College, Honors Research Projects

Many topics from conservation biology, evolutionary biology, and ecology will be applied to help answer the question many conservation biologists ask: is this population both genetically and ecologically distinct enough to warrant an independent conservation approach? If the answer is yes, then more resources can be distributed to conserving that population. If the answer is no, more resources can be devoted to other conservation goals, such as conserving one of the other three breeding populations of Hawaiian petrel or generally trying to maximize the overall population size of the species and possibly saving the endangered Hawaiian petrel. This will be …

Does The Type Of Housing A Child Lives In Predict Blood Lead Level?, Elizabeth Alvarado Navarro

Open Access Theses & Dissertations

Background and Significance: Childhood lead exposure continues to be a global health problem that can compromise children's long-term health. Identifying factors that predict which children may be at the highest risk of exposure is a strategy that could help focus child lead surveillance. Lead is a natural element found in small amounts in the earth's crust and has been widely used in industry and consumer products since the time of the Romans. Lead, however, is highly toxic for humans and serves no biological purpose in the human body. There is substantial evidence that, even at the lowest levels of exposure, …

Lévy Processes: Characterizing Volcanic And Financial Time Series, Peter Kwadwo Asante

Open Access Theses & Dissertations

In this work, we use the Diffusion Entropy Analysis (DEA) to analyze and detect the scaling properties of time series from both emerging and well established markets as well as volcanic eruptions recorded by a seismic station, both financial and volcanic time series data are known to have high frequencies (i.e they are collected at an extremely fine scale). The objective is to determine the characterization i.e whether they follow a Gaussian or Lévy distribution. If they do follow a Lévy distribution we are then interested in finding if they are characterized by a Lévy walk which has a finite …

Prediction And Classification Of G Protein-Coupled Receptors Using Statistical And Machine Learning Algorithms, Fredrick Ayivor

Open Access Theses & Dissertations

G protein-coupled receptors (GPCRs) are transmembrane proteins with important functions in signal transduction and often serve as therapeutic drug targets. With increasing availability of protein sequence information, there is much interest in computationally predicting GPCRs and classifying them to indicate their possible biological roles. Such predictions are cost-efficient and can be valuable guides for designing wet lab experiments to help elucidate signaling pathways and expedite drug discovery. There are existing computational tools for GPCR prediction and classification that involve statistical and machine learning approaches such as principal component analysis, support vector machines, hidden Markov models, etc. These tools use protein …

A Comparative Study Of The Impact Of Depth In Deep Learning Architectures, Kirsten Byers

Open Access Theses & Dissertations

Machine Learning continues to evolve as applications become more complex. Neural Networks, or Deep Networks, are integral to machine learning and the entire taxonomy of Artificial Intelligence [Sze17]. Intelligent structures and algorithms continue to advance, keeping pace with the complexi-ty of data. Changes in architecture, algorithms, and parameters are necessary to keep up with com-putational complexity and data available. This study focuses on how changes in depth of the archi-tecture affect performance on three distinct datasets, including one on Heart Disease. An adaptable network is created in original code, trained, and tested on these datasets. Its performance parameters are observed …

Development Of Advanced Statistical Methods For Multivariate Classification, Mario Cardenas Jr

Open Access Theses & Dissertations

This work is on the development of an observation reduction technique based on the principal component scores, or loadings, intended for the classification of data.

At The Mercy Of The Mexican Supreme Court: The Implications Of Party Capability On Indigenous People's Cases, Alan Cardenas

Open Access Theses & Dissertations

Indigenous Peoples in Mexico have long struggled in securing their rights in colonizing states. Applying party capability theory, this paper seeks to empirically understand the Mexican Supreme Court's behavior in cases pertaining to Indigenous Peoples. This paper thus evaluates the degree to which the Mexican Supreme Court is indeed an impartial actor that produces "equal protection under the law" for everyone (Galanter, 1974). Specifically, this paper examines the questions: To what extent does the Mexican Supreme Court protect Indigenous Peoples' rights? Are Indigenous Peoples legally affected by the power disparity perpetuated by the inequality in the country? This paper thus …

Regulation Of Schlafen 11 Expression, Christian Waldemar Corona Ayala

Open Access Theses & Dissertations

Schlafen 11 (SLFN11) is a member a family of proteins that can be found in mammals. Members of this family are involved in important functions. SLFN11 in particular has been identified as a restriction factor for several evolutionarily disparate viruses such as HIV-1 and Flaviviruses and to play an important role in DNA repair, sensitizing cells to chemotherapy. Despite its relevance SLFN11 remains largely uncharacterized. The aim of this research was to achieve a more comprehensive understanding the regulation mechanism of SLFN 11 expression. Before our research, it was only known that type I interferon (IFN) regulates SLFN11 levels. However, …

Association Of A Repetitive Manual Task On Inflammatory Biomarker Expression, Heart Rate Variability And Rating Of Perceived Exertion, Daniel Conde

Open Access Theses & Dissertations

The National Institute for Occupational Safety and Health (NIOSH) defines work related musculoskeletal disorders (WMSDs) as a group of disorders involving muscles, tendons, and nerves. The WMSDs symptoms are considered the most common occupational disease, causing limitations in daily activities. Even though there is an association between repetitive manual tasks involving short work cycles, force demanding tasks, and the development of WMSDs, the specific cause of the physiological changes over a long period of time is a cause of debate. The study of mechanisms underlying the physiological changes is essential for the early detection, diagnosis, and appropriate management of WMSDs.

To Or Through The Skin: A Rational Design Of Transdermal Drug Delivery, Isaac Giovanni Deaguero

Open Access Theses & Dissertations

The skin is the largest organ in the body and plays many crucial roles through diverse functions ranging from protection, temperature regulation, energy storage via fat deposits, and is used to communicate with our surroundings. Various methods have been attempted to deliver drugs to target areas of the skin in effective dosages by a variety of means with limited success. Recently, advances in transdermal drug delivery have offered avenues previously unattainable through conventional means. Some of these new delivery methods have been reviewed, explored and offer promising results with the potential to carve a new avenue in the way therapeutic …

Sensory Integration And Its Role In Behavioral Plasticity In Children Diagnosed With Autism Spectrum Disorder, Clarissa Diaz

Open Access Theses & Dissertations

Although commonly characterized by communicative and social impairments, Autism Spectrum Disorder (ASD) presents a number of developmental deficits related to movement planning and action. The extent of these deficits to the entire neuromuscular system, as well as the individual input/output loops are still not well understood. Given the dynamic interplay between our plans, actions and outcomes lay the foundation for later mature motor behavior, it is critical to understand the unique motor learning processes these nervous systems face while a higher level of training plasticity may be present. A recent study examined the kinematics (acceleration, velocity, smoothness, etc.) of upper …

The Effect Of Curriculum- And Classroom-Based Physical Activity Breaks On Academic Performance In Elementary School Children In Southern New Mexico, Sephonnie S. Elliss

Open Access Theses & Dissertations

Research has provided strong evidence for the many health benefits of exercise (e.g. decreased risk of heart disease, diabetes, osteoporosis, sleep apnea, falls). Despite the plethora of evidence, most Americans do not meet the Surgeon General's recommendation of achieving 150 minutes of moderate-to-vigorous physical activity (PA) per week. Compounding the problem for children is the fact that although most American kids are enrolled in school for 30 or more hours per week, making schools a prime location to teach modes of physically active health habits, schools have instead increasingly become places that promote sedentary behavior as greater emphasis has been …

Behavioral Ecology Of A Desert Ambush Predator: Assessing Movement Patterns, Habitat And Microhabitat Use, And The Innate Feeding Response Of Eastern Black-Tailed Rattlesnakes (Crotalus Ornatus) In The Northern Chihuahuan Desert, James Emerson

Open Access Theses & Dissertations

Research on the ecology and evolution of rattlesnakes has been sporadic over the past 80 years, but has seen recent expansion into the diverse fields of physiology, physiological ecology, life history, behavioral ecology, ethology, reproductive biology, chemical ecology, venom biochemistry and medicine, conservation, and many other subdisciplines. The development of small, implantable VHF radiotransmitters in the 1980s revolutionized research in the field of behavioral ecology for rattlesnakes, which are uniquely suited for radiotelemetry studies because they possess several morphological, physiological, and behavioral characteristics that are unique among terrestrial vertebrates. The widespread application of radiotelemetry by the mid-1980s advanced the model …

Analyzing Znf16: An Understudied Gene, Chelsea L. George

Open Access Theses & Dissertations

The zinc finger protein 16 (ZNF16) has been reported to be involved in megakaryocytic and erythroid differentiation. However, ZNF16 has ubiquitous expression throughout the body thus suggesting that it might have functions outside of these blood cell lineages. Here we show that ZNF16 is expressed in different tumor and non-tumor cell lines, consistent with the hypoThesis of a more widespread role. Endogenous ZNF16 localizes to the nucleus and is enriched in the nucleolus. Interestingly, exogenous ZNF16 showed two patterns of localization depending on the tag used. EGFP-ZNF16 showed nuclear localization while 3xEGFP-ZNF16 was mainly localized to the nucleoli and other …

Comparative Experimental And Theoretical Study Of Dopamine And Serotonin Interaction, Jose A. Guerrero

Open Access Theses & Dissertations

To accurately identify and measure the concentrations of dopamine (DA) and serotonin (5-HT) in mixtures of these neurotransmitters without labeling, a comprehensive, comparative computational and Raman experimental analysis is provided. While the distinction between these two analytes may be accomplished for concentrations in the millimolar range of these mixtures, their accurate quantification remains unattainable. As shown for the first time in this study, potential creation of a new composite resulting from their interactions with each other could be a reason for this lack of quantification.

Although this new hydrogen-bonded complex greatly complicates future analyte differentiation and quantification at concentrations typical …

Trials Of Belonging: Indigenous Peoples' Struggle With Law And Power In Twentieth-Century North America, Kevin Thomas Guay

Open Access Theses & Dissertations

Spanning multiple fields of scholarly inquiry, the bulk of this study concerns itself with competing notions of sovereignty, citizenship, boundary-making, and belonging in twentieth and twenty-first century Indigenous North America. Situated at the productive confluence of Borderlands history, Native American and Indigenous Studies, Critical Legal Studies, and Immigration history, this Dissertation analyzes the numerous treaties, codes, edicts, bylaws and other expressions of settler colonial jurisprudence that penetrated the everyday lives of Indigenous peoples across North America. These statutes-designed to limit Native power, dissolve Indigenous cultural identity, and strip tribal peoples of their landholdings and personhood-constituted an ongoing settler colonial project …

Region-Specific Modifications Of Synaptic Proteins In Response To Early-Life Adversity In The Rat Brain, Jameel Nasser Hamdan

Open Access Theses & Dissertations

Drug addiction is a serious condition affecting approximately 19.7 million people in the United States alone (SAMHSA 2018). Exposure to stress and other adverse conditions has been shown to impact drug-taking behavior and making individuals more vulnerable to addiction. In those already suffering from addiction, abstaining from drug use often gives way to relapse after a stressful event (Lu, Shepard et al. 2003; Shaham, Erb et al. 2000). The experience of adversity during early-life can cause long-lasting changes in the brain, affecting development, behavior, learning and memory, and critical thinking processes, which may persist into adulthood (Aisa, Tordera et al. …

A Cellulose-Based Biological Sensor For The Quantitative Determination Of Glucose And Glycated Hemoglobin (Hba1c), Gilberto Henao-Pabon

Open Access Theses & Dissertations

ABSTRACT

Importance: Diabetes is a common world-wide disease characterized by insulin resistance and as result there is an increased risk of cardiovascular disease and other metabolic abnormalities, that contribute to irreversible end-organ damage. The cause of diabetes is multifactorial and includes genetic, inflammatory and environmental factors plus lifestyle issues. Because in diabetes there are high levels of glucose and glycated hemoglobin, these molecules are used in clinical settings for the acute and chronic management of diabetic patients.

Objectives: a. To design and build an electrochemical biological sensor using the enzyme Glucose Oxidase (GOX) to detect glucose in biological fluids.

b. …

The Study Of The Mouse Schlafen Family Members As Mediators Of Antiviral Activity, Julia Fernanda Hernandez

Open Access Theses & Dissertations

The Schlafen family of proteins was first characterized in 1988, and since then evidence of their relevance in different physiological and pathological situations have been accumulating although at a slow pace. Hence, the biological roles of these proteins remain ill-defined. The expression of these proteins is positively regulated by type 1 interferons, constituting a component of the innate immune response. Functions of these proteins vary from regulation of cell proliferation to restriction of viral infections. Recent studies have implicated SLFN11 and SLFN13 as inhibitors of HIV-1 infection2-4. The mechanism of action of SLFN11 resides in its capacity to regulate the …

Isolation And Characterization Of Α-Gal-Containing Extracellular Vesicles (Evs) From Three Major Genotypes Of Trypanosoma Cruzi: Potential Biomarkers Of Chagas Disease, Nasim Karimi Hosseini

Open Access Theses & Dissertations

Chagas disease (ChD) is a neglected tropical disease (NTD) caused by the protozoan parasite, Trypanosoma cruzi. It is transmitted by the insect-vector triatomine (popular known as kissing bug), blood transfusion, organ transplantation, congenitally, and contaminated foods and juices. T. cruzi has evolved several strategies to invade the host cells, including the release extracellular vesicles (EVs), which assist pathogen survival and its replication within the host. T. cruzi is covered with highly glycosylated surface molecules such as glycoproteins and glycolipids, which are shown to be involved in the interaction with host immune cells. These molecules are highly immunogenic and reactive with …

Insights Into The Structure, Pharmacology, And Evolution Of The Glycine Transporter 2, Ashley Bryan Lopez

Open Access Theses & Dissertations

The balance between neuronal excitation and inhibition in the central nervous system is vital for survival. Overexcitation can be toxic and lead to certain manias or even death. On the other hand, extremely low levels of inhibition cause hyperekplexia, pain, and even forms of autism. Neuronal inhibition is, for the most part, achieved by two neurotransmitters, GABA and glycine. Levels of both neurotransmitters in the synaptic cleft are meticulously regulated by the GABA and glycine transporters, respectively, which belong to the solute carrier 6 (SLC6) family of neurotransmitter transporters and share structural similarities with other family members. In Chapter 1, …

Expression And Functional Sialome Of Triatomines, Insect Vectors Of Chagas Disease, Maria Tays Mendes

Open Access Theses & Dissertations

Triatomines are blood-sucking arthropods that transmit Trypanosoma cruzi, the etiologic agent of Chagas disease (ChD). Triatomines use bioactive molecules in the saliva for successful blood feeding and to evade the hemostatic and immune defense system of the hosts. Knowing the saliva composition could be useful for a better understanding which and how insect-derived molecules might influence host-parasite interactions. Previous studies have shown that some saliva-derived proteins and lipids can modulate the host immune system and increase T. cruzi infection. We hypothesize that the triatomine saliva contains a great diversity of lipids and proteins that can modulate the mammalian host immune …

Molecular And Functional Characterization Of Α-Galactosyl Epitopes In Trypanosoma Cruzi, Uriel Ortega Rodriguez

Open Access Theses & Dissertations

Trypanosoma cruzi, the causative agent of Chagas disease (CD) currently affects 6-7 million people across the world. Currently, only two drugs, benznidazole and nifurtimox, are available for treatment of CD and they are highly toxic and less effective in the chronic stage of the disease. Specific biomarkers for diagnosis and follow-up of treatment do not exist in the clinical settings. following chemotherapy, patients take approximately 10-20 years to exhibit negative seroconversion with the conventional serology assays. Moreover, there is no vaccine available to prevent or treat CD. T. cruzi contains a complex cell surface consisting of several classes of glycoconjugates …

"Celebrated, Not Tolerated": How Transgender And Nonbinary Individuals Navigate The Social World, Hannah Penklis

Open Access Theses & Dissertations

Seventeen individuals (4 trans men, 2 trans women, and 11 nonbinary people) were interviewed in this exploratory study on how transgender and nonbinary people feel about their bodies, and the role that social support plays in their experiences and feelings about their bodies. Semi-structured interviews revealed themes, including how transgender and nonbinary people interact with social support, their motivations for gender affirming medical interventions, and non-medical ways in which they alter their physical appearance. Participants reported a variety of experiences within their body across the spectrum of human experience: in some cases, individuals changed their weights dramatically; others felt strong …

Novel Tools For Community Based Assessment And Monitoring Of Forest Phenology Response To Environmental Variability And Change In The Tropics, Jennifer Christine Ramos-Chavez

Open Access Theses & Dissertations

Tropical ecosystems provide a variety of services and resources for global well-being. However, landscapes within this biome are undergoing rapid changes mostly due to anthropogenic activities. These systems are further impacted by current, extreme fluctuations in climate, including climate change and El Niño Southern Oscillation (ENSO). With predicted future increase in strength and occurrence of these climate oscillations, these landscapes and their plant communities will be impacted and changed. There is a gap in research and knowledge among tropical Indonesian landscapes in regards to the effects of global climate change at the community level. Therefore, there is an urgent need …