Digital Commons Network^™

Sinorhizobium Meliloti Changes Motility Phenotypes In The Presence Of Serum, Eris K. Bisal

Theses and Dissertations

Sinorhizobium meliloti is a nitrogen-fixing flagellated soil bacterium that engages in a mutual symbiotic relationship with the legume Medicago sativa. Using its flagella, S. meliloti is chemotactic in response to varying environmental cues thereby directed to a target, which is typically the root hairs of M. sativa. Lu et al, 2012 suggested that a signal from the plant can switch S. meliloti from a free-living flagellated microbe to a host-invading non-flagellated microbe, although this signal has not been identified. This switch is thought to occur inside curled root hairs of the plant, and after becoming non-flagellated, S. …

Traditional Knowledge In Taiwan: A Call For Greater Participation Of Indigenous Peoples In The Global Intellectual Property Marketplace, James M. Cooper

Vanderbilt Journal of Transnational Law

This Article explores the plight of the Aborigines of Taiwan and the legal protections that exist for their Traditional Knowledge. While Taiwan continues to face international isolation with a diminished number of states recognizing the Republic of China as the seat of China, the island's government has taken limited steps to recognize language, cultural, and economic rights of its Indigenous peoples. International law has not been helpful in protecting Traditional Knowledge, but Taiwan could use its vast economic resources and positive track record in protecting some of these rights to further its goals of international recognition. This Article details the …

Something Is Not Always Better Than Nothing: Problematizing Emerging Forms Of Jus Ad Bellum Argument, David Hughes, Yahli Shereshevsky

Vanderbilt Journal of Transnational Law

Since the adoption of the UN Charter, an unending debate concerning the permissible exceptions to the use of force prohibition has filled the pages of countless law reviews. The resulting legal regime, the jus ad bellum, has become increasingly strained as the international community faces new threats and encounters unforeseen scenarios. The post-war legal architecture is, so the debate goes, either insufficiently enabled to address contemporary challenges or consistently undermined by actors who seek exceptions to the strict limits placed upon state conduct. Debates regarding different instances when force is used exhibit a predictable pattern. Those that wish to limit …

Simulating A Computational Biological Model, Rather Than Reading, Elicits Changes In Brain Activity During Biological Reasoning, Caron Clark, Tomáš Helikar, Joseph T. Dauer

Department of Educational Psychology: Faculty Publications

The creation and analysis of models is integral to all scientific disciplines, and modeling is considered a core competency in undergraduate biology education. There remains a gap in understanding how modeling activities may support changes in students’ neural representations. The aim of this study was to evaluate the effects of simulating a model on undergraduates’ behavioral accuracy and neural response patterns when reasoning about biological systems. During brief tutorials, students (n = 30) either simulated a computer model or read expert analysis of a gene regulatory system. Subsequently, students underwent functional magnetic resonance imaging while responding to system-specific questions and …

Effects Of The Asian Clam, Corbicula Fluminea, On Native Freshwater Mussels And Biofilms, Kiersten Youngquist

Murray State Theses and Dissertations

As native, freshwater mussels continue to decline in the United States, successful conservation and management plans are essential for their survival. Understanding the drivers of native mussel declines, how the ecological roles mussels play within aquatic systems may be affected by declines, and the conservation status of our mussel fauna in different watersheds is critical to inform management and conservation efforts. The Asian clam (Corbicula fluminea, Müller, 1774) is an invasive species found throughout the United States that can reach densities of greater than 1000/m². Given these high densities, Asian clams have been linked to declines in native mussels …

Field Efficacy Of Soil Insecticides On Pyrethroid-Resistant Western Corn Rootworms (Diabrotica Virgifera Virgifera Leconte), Dariane Souza, Julie A. Peterson, Robert Wright, Lance Meinke

Department of Entomology: Faculty Publications

Background: Field-evolved pyrethroid resistance has been confirmed in western corn rootworm (WCR) populations collected from the United States (US) western Corn Belt. Resistance levels of WCR adults estimated in lab bioassays were confirmed to significantly reduce the efficacy of foliar-applied bifenthrin. The objective of the present study was to investigate the impact of WCR pyrethroid resistance levels on the performance of common soil-applied insecticide formulations (23.4% tefluthrin, 17.15% bifenthrin, and 0.1% cyfluthrin+2.0% tebupirimphos). Field trials were conducted in 2016 and 2017 in three Nebraska, US, counties (Saunders, Clay, and Keith) where distinct levels of WCR susceptibility topyrethroids (susceptible, moderately resistant, …

Description Of The Immature Stages Of Stenocrates Agricola Dechambre & Hardy (Coleoptera: Scarabaeidae: Dynastinae: Cyclocephalini) With Redescription Of The Adult And Discussion Of Its Tribal Position, Mario G, Ibarra-Polesel, Jhon C. Neita-Moreno, Brett Ratcliffe

Department of Entomology: Faculty Publications

The enigmatic genus Stenocrates Burmeister has 52 species that are widely distributed from Mexico to Argentina, but mainly in South America. In the present study, the larva of the third instar and pupa of Stenocrates agricola Dechambre & Hardy, 2004 are described and illustrated based on specimens from Argentina, this being the first description of immatures for the genus. The adult is also redescribed, significantly expanding the number of characters and providing information on the natural history of this species. In addition, 28 species of Cyclocephalini and 20 species of Pentodontini are morphologically compared, emphasizing the most useful larval characters …

Methods For Surveying Stable Fly Populations, David B. Taylor, Kyle Harrison, Junwei J. Zhu

Department of Entomology: Faculty Publications

Stable flies are among the most important pests of livestock throughout much of the world. Their painful bites induce costly behavioral and physiological stress responses and reduce productivity. Stable flies are anthropogenic and their population dynamics vary depending on agricultural and animal husbandry practices. Standardized sampling methods are needed to better identify the factors controlling stable fly populations, test novel control technologies, and determine optimal management strategies. The current study reviewed methods used for a long-term study of stable fly population dynamics in the central Great Plains. An additional study compared the relative size of flies sampled from the general …

Hear For You: Engaging With Vocal Authority And Vulnerability Through Podcasting, Rowen Light-Wills

Scripps Senior Theses

Through applications of literary and film theory, cultural analysis, and historical context this thesis project will deconstruct the text of the physical and metaphorical gendered voice. The podcast medium allows for exploration of the physical voice in a spontaneous format, which makes it the ideal platform for hosting conversations between college-aged students that examine cultural conceptions of the voice. Focusing primarily on the feminized[1] voice as a site of cultural tension, participants can tell the story of their voices, talking about experiences that have shaped their auditory self-perception. The work of this project is to demonstrate that the medium …

Unaccepted Streets In Bayview Hunters Point: Addressing Spatial Injustice Through Public Open Space, Clare Blackwell

Scripps Senior Theses

Bayview Hunters Point is at the epicenter of environmental justice issues in San Francisco. The district has historically been the location of the city’s most polluting industries, as well as some of its poorest residents and its greatest concentration of public housing. As the city’s zoning developed over the twentieth century, the southeastern neighborhood on the bay became a patchwork of industrial and residential parcels of land, fueling spatial injustice, in particular a lack of access to public open space.

Increasing greenspace would likely address spatial injustice in Bayview Hunters Point. However, green gentrification–the consequence of resident displacement as a …

The Path To Pregnancy: Fertility Services And Assisted Reproductive Technologies, Olivia H. Gilbert

Scripps Senior Theses

The emergence of reproductive assistance like fertility treatments and assisted reproductive technologies (ART) in recent decades have provided new paths to pregnancy for many individuals. However, certain demographics in the US like low-income women and women of color are often excluded from utilizing these services on the basis of race, geography, and socioeconomic status. This in turn leads to significant disparities in access to and usage rates of such services. This research points out the perceived shortcomings in the current academic discussion surrounding fertility service disparities with the ultimate goal of expanding access to fertility assistance services for those who …

Retrieval-Induced Forgetting In Autism Spectrum: Combining Narrative Experience With Clinical Research To Explore Stress-Induced, Transitory Retrograde Amnesia, Elizabeth Willsmore-Finkle

Scripps Senior Theses

Currently, psychological research explores autism, a blanket term for a range of neurobiological and developmental differences, through a clinical, as opposed to an experiential, lens. Autism has only existed as formal diagnosis under that name since 1943 (Kanner); however, the advocacy of activists such as Temple Grandin, a slaughterhouse systems designer best known for documenting her life with autism in a series of autobiographic accounts, has begun to legitimize the incorporation of emic experiences of autism within clinical research. Researcher Dermot Bowler and colleagues (2011) have conducted extensive reviews of memory distinctions in autism, finding differences of varying degrees across …

Complex Ciliary Flows Around Stentor Polymorphus In Solutions Of 2% Buttermilk And Chlamydomonas Reinhardtii, Eliana B. Smithstein

Scripps Senior Theses

Stentor are large, unicellular ciliates of the Heterotricha order. They live in both freshwater and marine habitats and are mostly found in ponds. I studied Stentor polymorphus, which is a species of Stentor only recently discovered to be lab culturable. They range from 0.5-1.5mm in length and are unusual because they live with endosymbiotic algae and are much more likely than other, more widely studied, species of Stentor to form aggregates while they are eating. There are three main components to this thesis: First, I established protocols for keeping a viable S. polymorphus culture, since no protocols had been …

Neuroscience Of Stress & Addiction: A Digitally Animated Video On How These Brain Systems Interact And Influence Each Other From Early Life Stress To Withdrawal, Claire Bacon-Brenes

Scripps Senior Theses

Alcohol addiction and stress are both highly prevalent health conditions impacting our society. With stress on the rise in college students and alcohol addiction impacting 19.7 million Americans as of 2017, communication around the science of these issues is ever important because of stigma around them. This digitally animated video effectively explains alcohol addiction and stress on a neurobiological level with college science students as the audience, including two important examples of how stress and alcohol addiction interact. Stress experienced early in life is a known risk factor in developing addiction due to dysregulation of the reward pathway and altered …

Intercropping For Water Conservation: Environmental And Economic Implications Of A Sustainable Farming Practice In California's Central Valley, Sophie Baker

Scripps Senior Theses

California’s agricultural sector is the biggest water consumer in the state and faces intense pressure to reduce its overall water usage. Industrialized monoculture systems dominate the industry and often disregard long-term environmental and economic externalities for short-term profit maximization. To maintain longstanding food security and economic stability as well as protect the state’s water supply, it is critical that these systems transition to more sustainable and resilient production mechanisms. As an alternative to monoculture, intercropping affords greater potential to conserve water, protect soil quality, and increase crop yields, among other metrics of sustainability. However, there has been much controversy over …

(Re)Reading Fanon: Tracing Revolutionary Negotiations Within The Algerian Colonial Dialectic, Nina Zietlow

Scripps Senior Theses

A critical rereading of Fanon within the Algerian colonial context.

Terpenoid-Induced Feeding Deterrence And Antennal Response Of Honey Bees, Nicholas R. Larson, Scott T. O'Neal, Ulrich R. Bernier, Jeffrey R. Bloomquist, Troy D. Anderson

Department of Entomology: Faculty Publications

Multiple interacting stressors negatively affect the survival and productivity of managed honey bee colonies. Pesticides remain a primary concern for beekeepers, as even sublethal exposures can reduce bee immunocompetence, impair navigation, and reduce social communication. Pollinator protection focuses on pesticide application guidelines; however, a more active protection strategy is needed. One possible approach is the use of feeding deterrents that can be delivered as an additive during pesticide application. The goal of this study was to validate a laboratory assay designed to rapidly screen compounds for behavioral changes related to feeding or feeding deterrence. The results of this investigation demonstrated …

Ento(O)Mics: The Intersection Of ‘Omic’ Approaches To Decipher Plant Defense Against Sap-Sucking Insect Pests, Prince Zogli, Lise Pingault, Sajjan Grover, Joe Louis

Department of Entomology: Faculty Publications

Plants are constantly challenged by insect pests that can dramatically decrease yields. Insects with piercing-sucking mouthparts, for example, aphids, whiteflies, and leaf hoppers, seemingly cause less physical damage to tissues, however, they feed on the plant’s sap by piercing plant tissue and extracting plant fluids, thereby transmitting several plantpathogenic viruses as well. As a counter-defense, plants activate an array of dynamic defense machineries against insect pests including the rapid reprogramming of the host cell processes. For a holistic understanding of plant-sap-sucking insect interactions, there is a need to call for techniques with the capacity to concomitantly capture these dynamic changes. …

Phenology And Dispersal Of The Wheat Stem Sawfly (Hymenoptera: Cephidae) Into Winter Wheat Fields In Nebraska, Chris T. Mccullough, Gary L. Hein, Jeff Bradshaw Dr.

Department of Entomology: Faculty Publications

Historically, the wheat stem sawfly, Cephus cinctus Norton was a pest in spring wheat-growing regions of the northern Great Plains. However, in the 1980s, it was found infesting winter wheat fields in Montana. Infestations were first detected in western Nebraska in the 1990s, and have since spread throughout the Nebraska Panhandle. Larval damage occurs from stem-mining, but stem girdling that results in lodged stems that are not harvested results in the greatest yield losses.The biology and phenology of the wheat stem sawfly are well described in the northern portion of its range, but they are lacking in Colorado, southeast Wyoming, …

Genome Sequence Of A Small Rna Virus Of The Southern Corn Rootworm, Diabrotica Undecimpunctata Howardi Barber (Coleoptera: Chrysomelidae), Sijun Liu, Arnubio Valencia-Jiménez, Molly Darlington, Ana M. Vélez, Bryony C. Bonning

Department of Entomology: Faculty Publications

The genome sequence of a novel small RNA virus, tentatively named Diabrotica undecimpunctata virus 1 (DuV1), was discovered from the transcriptome of the southern corn rootworm, Diabrotica undecimpunctata howardi Barber. DuV1 has a positive-sense, single-stranded RNA genome that encodes a single polyprotein of 3,401 amino acids with limited similarity to other viruses.

Maize Defense Elicitor, 12-Oxo-Phytodienoic Acid, Prolongs Aphid Salivation, Sajjan Grover, Suresh Varsani, Michael V. Kolomiets, Joe Louis

Department of Entomology: Faculty Publications

12-Oxo-phytodienoic acid (OPDA), an intermediate in the jasmonic acid (JA) biosynthesis pathway, regulates diverse signaling functions in plants, including enhanced resistance to insect pests. We previously demonstrated that OPDA promoted enhanced callose accumulation and heightened resistance to corn leaf aphid (CLA; Rhopalosiphum maidis), a phloem sap-sucking insect pest of maize (Zea mays). In this study, we used the electrical penetration graph (EPG) technique to monitor and quantify the different CLA feeding patterns on the maize JA-deficient 12-oxo- phytodienoic acid reductase (opr7opr8) plants. CLA feeding behavior was unaffected on B73, opr7opr8 control plants (- OPDA), and opr7opr8 plants that were pretreated …

Greenbug (Schizaphis Graminum) Herbivory Significantly Impacts Protein And Phosphorylation Abundance In Switchgrass (Panicum Virgatum), Prince Zogli, Sophie Alvarez, Michael J. Naldrett, Nathan A. Palmer, Kyle G. Koch, Lise Pingault, Jeffrey D. Bradshaw, Paul Twigg, Tiffany M. Heng‐Moss, Joe Louis, Gautam Sarath, Joe Louis

Department of Entomology: Faculty Publications

Switchgrass (Panicum virgatum L.) is an important crop for biofuel production but it also serves as host for greenbugs (Schizaphis graminum Rondani; GB). Although transcriptomic studies have been done to infer the molecular mechanisms of plant defense against GB, little is known about the effect of GB infestation on the switchgrass protein expression and phosphorylation regulation. The global response of the switchgrass cultivar Summer proteome and phosphoproteome was monitored

by label‐free proteomics shotgun in GB‐infested and uninfested control plants at 10 days post infestation. Peptides matching a total of 3,594 proteins were identified and 429 were differentially expressed proteins in …

Spatial Repellency, Antifeedant Activity And Toxicity Of Three Medium Chain Fatty Acids And Their Methyl Esters Of Coconut Fatty Acid Against Stable Flies, Gwang H. Roh, Xiaaojie Zhou, Yansu Wang, Steven C. Cermak, James A. Kenar, Alexander Lehmann, Baoyu Han, David B. Taylor, Xiaopeng Zeng, Chung Gyoo Park, G. J. Brewer, J.J. Zhu

Department of Entomology: Faculty Publications

BACKGROUND: Stable flies are one of the most detrimental arthropod pests to livestock. With changing climates and agronomic practices, they expand their roles as pests and disease vectors as well. Their painful bites reduce livestock productivity, annoy companion animals, and interfere with human recreational activities. Current management technologies are unable to effectively control stable flies. The present study reports new results concerning the contact, spatial repellency, and toxicity of a bio-based product, coconut fatty acid and their methyl ester derivatives of free fatty acids of C8:0, C10:0 and C12:0 to stable flies.

RESULTS: Three medium chain fatty acid methyl esters …

Abundance And Diversity Of Dung Beetles (Coleoptera: Scarabaeoidea) As Affected By Grazing Management In The Nebraska Sandhills Ecosystem, Patrick M. Wagner, Gandura Omar Abagandura, Martha Mamo, Thomas Weissling, Ana Wingeyer, Jeffrey D. Bradshaw

Department of Entomology: Faculty Publications

Dung beetles (Coleoptera: Scarabaeoidea) serve a significant role in regulating ecosystem services on rangelands. However, the influence of grazing management on dung beetle communities remains largely unknown. The purpose of this study was to investigate dung beetle abundance and diversity throughout the grazing season in the Nebraska Sandhills Ecoregion. Grazing treatments included: continuous grazing (CONT), low-stocking rotational grazing (LSR), high-stocking rotational grazing (HSR), and no grazing (NG). The abundance and diversity of dung beetles were measured in the 2014 and 2015 grazing seasons using dung-baited pitfall traps. Dung beetle abundance for each grazing treatment was characterized through four indices: peak …

Sublethal Endpoints In Non-Target Organism Testing For Insect-Active Ge Crops, Andrew Roberts, Chad J. Boeckman, Marina Mühl, Jörg Romeis, John L. Teem, Fernando H. Valicente, Judith K. Brown, Steven L. Levine, Richard L. Melnick, Thais B. Rodrigues, Ana M. Vélez, Xuguo Zhou, Richard L. Hellmich, Martin G. Edwards

Department of Entomology: Faculty Publications

Historically, genetically engineered (GE) plants that have incorporated genes conferring insect protection have primarily used Cry proteins derived from Bacillus thuringiensis (Bt) to achieve their insecticidal phenotype. As a result, regulators have developed a level of familiarity and confidence in reviewing plants incorporating these insecticidal proteins. However, new technologies have been developed that produce GE plants that incorporate pest protection by triggering an RNA interference (RNAi) response or proteins other than Bt Cry proteins. These technologies have new modes of action. Although the overall assessment paradigm for GE plants is robust, there are ongoing discussions about the appropriate tests and …

Career Funneling, Perceptions Of Success, And Their Impact On College Students At Scripps, Pitzer, And Claremont Mckenna Colleges, Carina A. Schick

Scripps Senior Theses

The U.S. News top college ranking lists have created a narrowing definition of collegiate and career success. Students are told an elite education is the ticket to a successful life, one filled with a high achieving career, meaning, and happiness. Through peer, familial, and media interfaces students are inundated with societal definitions of success such as fame, wealth, and status. Socialization primes adolescents to work towards these goals. This idealized type of success is only accessible to a select few, leading to dissatisfaction and creating pressures on students to work towards their college admission at early ages. This thesis examines …

Childhood Obesity In California: The Impact Of School Lunch Options And Physical Education Standards In Public Elementary Schools, Audrey Connell

Scripps Senior Theses

Obesity is a biosocial phenomenon in that it is shaped by both biological and social processes. On the biological level, excess body fat increases one’s risk of placing the body in a non-homeostatic state that can weaken the immune response. On the social level, social inequalities are linked to obesity in the United States where racial and ethnic minority communities with low education and high poverty rates bear the largest burden of obesity. In various institutions, multiple actors such as food marketers, public health officials, policy makers, and school administrators dictate the opportunities available to children for them to reach …

Merde, Merde, Merde: The Testing Effect, Foreign Language Anxiety, And Their Impact On Foreign Language Learning, Priya Canzius

Scripps Senior Theses

This experiment examined the effect of foreign language classroom anxiety and assigned learning condition (repeated studying or repeated testing) on participant recall of newly-learned foreign language vocabulary word pairs. Previous research has identified a testing effect, which is a phenomenon where repeated testing is better for a learner’s long-term recall than repeated studying. The hypothesis for this experiment was that the testing effect would occur for students with lower levels of foreign language classroom anxiety but not for students with higher levels of foreign language classroom anxiety. Sixteen participants recruited from Introductory French and Introductory Arabic classrooms at the Claremont …

Improved Sentinel Method For Surveillance And Collection Of Filth Fly Parasitoids, Christopher J. Geden, Dana M. Johnson, David B. Taylor

Department of Entomology: Faculty Publications

Parasitoids are important natural enemies of house flies and other muscoid flies. The two most commonly used methods for collecting fly parasitoids from the field have distinct advantages and disadvantages. Collections of wild puparia depend on the ability to find puparia in sufficient numbers and are prone to localized distortions in relative species abundance because of the overrepresentation of samples from hot spots of fly larval activity. Placement and retrieval of sentinel puparia is convenient and allows consistent sampling over time but is strongly biased in favor of Muscidifurax spp. over Spalangia spp. An improved sentinel method is described that …

Proceedings Of The 2020 American Bee Research Conference, Bradley N. Metz, Judy Wu-Smart, Michael Simone-Finstrom

Department of Entomology: Faculty Publications

The 2020 American Bee Research Conference (ABRC) was held on 9–10 January 2020 in conjunction with the annual convention of the American Beekeeping Federation Conference and Trade Show in Schaumburg, IL. Over the two-day conference, a total of 65 oral and poster presentations were given, representing work done from over 30 different research groups from throughout the United States and Canada. These proceedings contain the submitted abstracts for presentations given at the 2020 American Bee Research Conference.