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Board Of Regents Meeting Materials, June 18, 2020, Eastern Michigan University

Board of Regents Meeting Materials

No abstract provided.

Board Of Regents Meeting Materials, April 23, 2020, Eastern Michigan University

Board of Regents Meeting Materials

No abstract provided.

Board Of Regents Meeting Materials, December 10, 2020, Eastern Michigan University

Board of Regents Meeting Materials

No abstract provided.

Early Diagnosis And Intervention In Cystic Fibrosis: Imagining The Unimaginable, Andrea M Coverstone, Thomas W Ferkol

Open Access Publications

Cystic fibrosis is the most common life-shortening genetic disease affecting Caucasians, clinically manifested by fat malabsorption, poor growth and nutrition, and recurrent sinopulmonary infections. Newborn screening programs for cystic fibrosis are now implemented throughout the United States and in many nations worldwide. Early diagnosis and interventions have led to improved clinical outcomes for people with cystic fibrosis. Newer cystic fibrosis transmembrane conductance regulator potentiators and correctors with mutation-specific effects have increasingly been used in children, and these agents are revolutionizing care. Indeed, it is possible that highly effective modulator therapy used early in life could profoundly affect the trajectory of …

Direct Neuronal Reprogramming Of Common Marmoset Fibroblasts By Ascl1, Microrna-9/9*, And Microrna-124 Overexpression, Akisa Nemoto, Reona Kobayashi, Sho Yoshimatsu, Yuta Sato, Takahiro Kondo, Andrew S Yoo, Seiji Shiozawa, Hideyuki Okano

Open Access Publications

The common marmoset (

Characterization Of Missense Mutations In The Signal Peptide And Propeptide Of Fix In Hemophilia B By A Cell-Based Assay, Wenwen Gao, Yaqi Xu, Hongli Liu, Meng Gao, Qing Cao, Yiyi Wang, Longteng Cui, Rong Huang, Yan Shen, Sanqiang Li, Haiping Yang, Yixiang Chen, Chaokun Li, Haichuan Yu, Weikai Li, Guomin Shen

Open Access Publications

No abstract provided.

Relationships Between Big-Five Personality Factors And Alzheimer's Disease Pathology In Autosomal Dominant Alzheimer's Disease, Andrew J Aschenbrenner, Jennifer Petros, Eric Mcdade, Guoqiao Wang, David A Balota, Tammie Ls Benzinger, Carlos Cruchaga, Chengjie Xiong, Richard Perrin, Anne M Fagan, John C Morris, Randall Bateman, Jason Hassenstab, Et Al

Open Access Publications

Introduction: Changes in personality characteristics are associated with the onset of symptoms in Alzheimer's disease (AD) and may even precede clinical diagnosis. However, personality changes caused by disease progression can be difficult to separate from changes that occur with normal aging. The Dominantly Inherited Alzheimer Network (DIAN) provides a unique cohort in which to relate measures of personality traits to in vivo markers of disease in a much younger sample than in typical late onset AD.

Methods: Personality traits measured with the International Personality Item Pool at baseline from DIAN participants were analyzed as a function of estimated years to …

Population And Harvest Dynamics Of Midcontinent Sandhill Cranes, Aaron T. Pearse, Glen A. Sargeant, Gary L. Krapu, David A. Brandt

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Sandhill cranes (Antigone canadensis) inhabiting the midcontinent of North America have been hunted since the 1960s under management goals of maintaining abundance, retaining geographic distribution, and maximizing sustainable harvest. Some biologists have raised concerns regarding harvest sustainability because sandhill cranes have lower reproductive rates than other game birds. We summarized demographic information in an age‐structured matrix model to better understand population dynamics and harvest. Population indices and recovered harvest since the early 1980s suggest midcontinent sandhill cranes have experienced an average long‐term annual growth of 0.9%; meanwhile, harvest has increased 1.8% annually. Adult survival and recruitment rates estimated from field …

Therapeutic Enhancement Of Blood-Brain And Blood-Tumor Barriers Permeability By Laser Interstitial Thermal Therapy, Afshin Salehi, Mounica R Paturu, Bhuvic Patel, Matthew D Cain, Tatenda Mahlokozera, Alicia B Yang, Tsen-Hsuan Lin, Eric C Leuthardt, Hiroko Yano, Sheng-Kwei Song, Robyn S Klein, Robert Schmidt, Albert H Kim

Open Access Publications

BACKGROUND: The blood-brain and blood-tumor barriers (BBB and BTB), which restrict the entry of most drugs into the brain and tumor, respectively, are a significant challenge in the treatment of glioblastoma. Laser interstitial thermal therapy (LITT) is a minimally invasive surgical technique increasingly used clinically for tumor cell ablation. Recent evidence suggests that LITT might locally disrupt BBB integrity, creating a potential therapeutic window of opportunity to deliver otherwise brain-impermeant agents.

METHODS: We established a LITT mouse model to test if laser therapy can increase BBB/BTB permeability in vivo. Mice underwent orthotopic glioblastoma tumor implantation followed by LITT in combination …

Macrophage Lc3-Associated Phagocytosis Is An Immune Defense Against Streptococcus Pneumoniae That Diminishes With Host Aging, Megumi Inomata, Shuying Xu, Pallavi Chandra, Simin N. Meydani, Genzou Takemura, Jennifer A. Philips, John M. Leong

Open Access Publications

No abstract provided.

Defining And Conceptualizing Outcomes For De-Implementation: Key Distinctions From Implementation Outcomes, Beth Prusaczyk, Taren Swindle, Geoffrey Curran

Open Access Publications

BACKGROUND: Increasingly, scholars argue that de-implementation is a distinct concept from implementation; factors contributing to stopping a current practice might be distinct from those that encourage adoption of a new one. One such distinction is related to de-implementation

CONCEPTUALIZATION OF OUTCOMES: We conceptualize each of the outcomes from Proctor and colleagues' taxonomy of implementation outcomes for de-implementation research. First, we suggest key considerations for researchers assessing de-implementation outcomes, such as considering how the cultural or historical significance to the practice may impact de-implementation success and, as others have stated, the importance of the patient in driving healthcare overuse. Second, we …

Induction Of Wnt16 Via Peptide-Mrna Nanoparticle-Based Delivery Maintains Cartilage Homeostasis, Huimin Yan, Ying Hu, Antonina Akk, Muhammad Farooq Rai, Hua Pan, Samuel A Wickline, Christine T N Pham

Open Access Publications

Osteoarthritis (OA) is a progressive joint disease that causes significant disability and pain and for which there are limited treatment options. We posit that delivery of anabolic factors that protect and maintain cartilage homeostasis will halt or retard OA progression. We employ a peptide-based nanoplatform to deliver Wingless and the name Int-1 (WNT) 16 messenger RNA (mRNA) to human cartilage explants. The peptide forms a self-assembled nanocomplex of approximately 65 nm in size when incubated with WNT16 mRNA. The complex is further stabilized with hyaluronic acid (HA) for enhanced cellular uptake. Delivery of peptide-WNT16 mRNA nanocomplex to human cartilage explants …

Estimating Abiotic Thresholds For Sagebrush Condition Class In The Western United States, Stephen Boyte, Bruce K. Wylie, Yingxin Gu, Donald J. Major

United States Geological Survey: Staff Publications

Sagebrush ecosystems of the western United States can transition from extended periods of relatively stable conditions to rapid ecological change if acute disturbances occur. Areas dominated by native sagebrush can transition from species-rich native systems to altered states where non-native annual grasses dominate, if resistance to annual grasses is low. The non-native annual grasses provide relatively little value to wildlife, livestock, and humans and function as fuel that increases fire frequency. The more land area covered by annual grasses, the higher the potential for fire, thus reducing the potential for native vegetation to reestablish, even when applying restoration treatments. Mapping …

Mcnair Scholars Research Journal 2020, Michelle X. Bui, Chris Mcdaniels, Jacqueline Bazea-Rubio, Curtis Kizer-Pugh, Eman Alasadi, Ahmed Gure, Helen Hernandez, Archit Jaiswal, Dallas Johnson, Ephrem Kejela, Heather Lake, Charlene Mandimutsira, Paola A. Puentes, Anthony Sajik Ruiz, Richard D. Schargel, Thao-My Tran, Omar Yanouri

McNair Scholars Research Journal-Archive

No abstract provided.

Host Transcriptional Response To Persistent Infection With A Live-Attenuated Porcine Reproductive And Respiratory Syndrome Virus Strain, Jayeshbhai Chaudhari, Chia-Sin Liew, Aspen M. Workman, Jean-Jack Riethoven, David J. Steffen, Sarah Vitosh-Sillman, Hiep Vu

Nebraska Center for Virology: Faculty Publications

Both virulent and live-attenuated porcine reproductive and respiratory syndrome virus (PRRSV) strains can establish persistent infection in lymphoid tissues of pigs. To investigate the mechanisms of PRRSV persistence, we performed a transcriptional analysis of inguinal lymphoid tissue collected from pigs experimentally infected with an attenuated PRRSV strain at 46 days post infection. A total of 6404 differentially expressed genes (DEGs) were detected of which 3960 DEGs were upregulated and 2444 DEGs were downregulated. Specifically, genes involved in innate immune responses and chemokines and receptors associated with T-cell homing to lymphoid tissues were down regulated. As a result, homing of virus-specific …

First Records Of Established Populations Of Ixodes Scapularis (Acari: Ixodidae) Collected From Three Nebraska Counties, Lindsey E. Nielsen, Roberto Cortinas, Paul D. Fey, Peter C. Iwen, David H. Nielsen

School of Veterinary and Biomedical Sciences: Faculty Publications

Reported cases of Lyme disease in Nebraska have been assumed to be imported from other endemic areas. Previous surveillance efforts provided no evidence of established populations as only individual specimens of Ixodes scapularis (Say) had been collected. In the winter of 2018, adult I. scapularis were found on a dog at Two Rivers State Recreation Area, Douglas County, prompting tick collection at the site and nearby natural areas. In May 2019, all life stages of host-seeking I. scapularis were collected using dragging and flagging techniques in sites located near the Platte River in Douglas, Sarpy, and Saunders counties. This is …

Intimate Attachment Of Escherichia Coli O157:H7 To Urinary Bladder Epithelium In The Gnotobiotic Piglet Model, Rodney A. Moxley, Tom W. Bargar, Stephen D. Kachman, Diane R. Baker, David H. Francis

School of Veterinary and Biomedical Sciences: Faculty Publications

Enterohemorrhagic Escherichia coli (EHEC), a pathogenic subset of Shiga toxin-producing E. coli (STEC), is an important cause of hemorrhagic colitis and hemolytic–uremic syndrome (HUS), and a rare cause of urinary tract infections (UTIs) with associated HUS. EHEC strains attach intimately to intestinal epithelium with formation of actin pedestals (attaching-effacing (A/E) lesions); however, the mechanism of EHEC attachment to the uroepithelium is unknown. We conducted a retrospective study on archived urinary bladder specimens from gnotobiotic piglets that naturally developed cystitis associated with EHEC O157:H7 infection following oral inoculation and fecal shedding. Paraffin-embedded bladder tissues from three piglets with cystitis and immunohistochemical …

Triggers Of Inflammatory Heart Disease, Ninaad Lasrado, Bharathi Yalaka, Jay Reddy

School of Veterinary and Biomedical Sciences: Faculty Publications

Inflammatory heart disease (IHD) is a group of diseases that includes pericarditis, myocarditis, and endocarditis. Although males appear to be more commonly affected than females, IHD can be seen in any age group. While the disease can be self-limiting leading to full recovery, affected individuals can develop chronic disease, suggesting that identification of primary triggers is critical for successful therapies. Adding to this complexity, however, is the fact that IHD can be triggered by a variety of infectious and non-infectious causes that can also occur as secondary events to primary insults. In this review, we discuss the immunological insights into …

Current Status Of Zika Virus Vaccines: Successes And Challenges, Aryamav Pattnaik, Bikash R. Sahoo, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

The recently emerged Zika virus (ZIKV) spread to the Americas, causing a spectrum of congenital diseases including microcephaly in newborn and Guillain-Barré syndrome (GBS) in adults. The unprecedented nature of the epidemic and serious diseases associated with the viral infections prompted the global research community to understand the immunopathogenic mechanisms of the virus and rapidly develop safe and efficacious vaccines. This has led to a number of ZIKV vaccine candidates that have shown significant promise in human clinical trials. These candidates include nucleic acid vaccines, inactivated vaccines, viral-vectored vaccines, and attenuated vaccines. Additionally, a number of vaccine candidates have been …

Rapid Differentiation Of Moraxella Bovoculi Genotypes 1 And 2 Using Maldi-Tof Mass Spectrometry Profiles, Matthew M. Hille, Aaron M. Dickey, Kara Robbins, Michael L. Clawson, John Dustin Loy

School of Veterinary and Biomedical Sciences: Faculty Publications

Moraxella bovoculi is the most frequently isolated bacteria from the eyes of cattle with Infectious Bovine Keratoconjunctivitis (IBK), also known as bovine pinkeye. Two distinct genotypes of M. bovoculi, genotype 1 and genotype 2, were characterized after whole genome sequencing showed a large degree of single nucleotide polymorphism (SNP) diversity within the species. To date, both genotypes have been isolated from the eyes of cattle without clinical signs of IBK while only genotype 1 strains have been isolated from the eyes of cattle with clinical signs of IBK. We used 38 known genotype 1 strains and 26 known genotype …

Viscacha: Luxury, Fate And Identification In Precolumbian Textiles, Elena Phipps, Caroline Solazzo

Textile Society of America: Symposium Proceedings

Since the sixteenth century, references by Spanish chroniclers to the use of chinchilla hair (Andean rodents of several species including viscacha) by Andean weavers indicate that the fine hair of these animals had been incorporated into textiles. Their fine soft hair, often mottled in color, has distinctive characteristics which vary and, as a result, make them difficult to identify. Some archaeological finds, primarily in Chile have reported viscacha skins and fur bags, but few if any reports have identified the presence of textiles or spun yarns that incorporate this special fiber. The depiction of the animal takes on special meaning …

Geographic Distribution Of Bacillus Thuringiensis Cry1f Toxin Resistance In Western Bean Cutworm (Lepidoptera: Noctuidae) Populations In The United States, Brad S. Coates, Craig A. Abel, Katharine A. Swoboda-Bhattarai, Debra E. Palmquist, Débora Goulart Montezano, Sarah N. Zukoff, Yangzhou Wang, Jeffery D. Bradshaw, Christina D. Difonzo, Elson Shields, Kelley J. Tilmon, Thomas Hunt, Julie A. Peterson

West Central Research and Extension Center, North Platte

The western bean cutworm (WBC), Striacosta albicosta (Lepidoptera: Noctuidae), can be a severe pest of transgenic corn in the western Plains and Great Lakes regions of North America, including on hybrids expressing the Bacillus thuringiensis (Bt) Cry1F toxin. The level and geographic distribution of Cry1F resistance are not completely known. Neonate S. albicosta from 10 locations between Nebraska and New York state were subjected to dose–response trypsin-activated native Cry1F toxin overlay bioassays. In 2017, the mean estimated lethal concentration causing 50% larval mortality (LC50) ranged from 15.1 to 18.4 μg Cry1F cm−2, and were not significantly different among locations. In …

Agroecosystems Entomology Lab, Research Summary 2020

West Central Research and Extension Center, North Platte

Contents

Introduction: Agroecosystems Entomology Lab

Project # 1: Chemigation efficacy and spray deposition on corn for the control of western bean cutworm

Project #2: Insecticide application on western bean cutworm eggs and response of predators to treated eggs

Project #3: Investigating the Role of Spiders in Integrated Pest Management for Biological Control of Nebraska Crop Pests

Project #4: Modeling the potential global geographic distribution of Striacosta albicosta using MaxEnt

Project #5: Diversity and abundance of arthropods in industrial hemp fields of Nebraska

Transcriptomic Data-Driven Discovery Of Global Regulatory Features Of Rice Seeds Developing Under Heat Stress, Mohammad Mazharul Islam, Jaspreet Sandhu, Harkamal Walia, Rajib Saha

Department of Chemical and Biomolecular Engineering: Faculty Publications

Plants respond to abiotic stressors through a suite of strategies including differential regulation of stress- responsive genes. Hence, characterizing the influences of the relevant global regulators or on stress- related transcription factors is critical to understand plant stress response. Rice seed development is highly sensitive to elevated temperatures. To elucidate the extent and directional hierarchy of gene reg- ulation in rice seeds under heat stress, we developed and implemented a robust multi-level optimization- based algorithm called Minimal Regulatory Network identifier (MiReN). MiReN could predict the minimal regulatory relationship between a gene and its potential regulators from our temporal transcriptomic dataset. …

Environmental Factors On The Arctic Food Chain, Sydney Hansen

Department of Environmental Studies: Undergraduate Student Theses

As the Arctic is encountering many environmental changes, a multi-species meta-analysis was conducted from peer-reviewed scientific data gathered from the University of Nebraska-Lincoln (UNL) library’s bibliographic databases to determine 1) the main polar warming impacts on species of the Arctic food web such as polar bears, ringed seals, Arctic cod, copepods, and primary producers, 2) determine climatic impacts by looking at population sizes and migratory patterns over several years, and 3) discover impacts of a top-down cascade and look into conservation efforts. In the Arctic, species within its’ ecosystem are experiencing more dramatic impacts from climatic warming because of the …

Nutritional Value Of Crops Affected By Elevated Carbon Dioxide Concentrations In Atmospheric Conditions, Alex Joseph Cusimano

Department of Environmental Studies: Undergraduate Student Theses

This research was conducted to evaluate the effects of rising atmospheric carbon dioxide concentrations, and the nutritional quality of crops. Using Free Air CO2 Enrichment (FACE) and Decision Support System for Agrotechnology Transfer (DSSAT) to simulate elevated carbon dioxide levels, more review of previous literature was studied. Studies found that although there is a negative loss of nutrient availability, there are major signs that nutrient deficiencies. Nitrogen(protein), Iron, and Zinc are the most impacted with elevated atmospheric CO2 conditions. Further research and experiments regarding long term plant growth should be considered moving forward. With only limted situations and different CO2 …

Analysis Of Trees Damaged From Flooding And Ice In Columbus, Nebraska, Alaina Kapla

Department of Environmental Studies: Undergraduate Student Theses

In the spring of 2019, an intense storm system came through eastern Nebraska resulting in a historic flooding event. This flood caused many trees throughout the state to be uprooted, broken, or scarred. This study looks at trees on the Quail Run golf course in Columbus, Nebraska to determine the extent of damage on trees caused by flooding and recommend tree care solutions to ensure safety. The cottonwood trees endured the most damage compared to other species on the property. All of the trees showed wound wood meaning that the trees were recovering from their injuries. Dead and broken branches …

Fungal Mycelium: The Key To A Sustainable Future, Sawyer Krivanek

Department of Environmental Studies: Undergraduate Student Theses

This study focuses on fungal mycelium-based packaging as a biodegradable replacement of polystyrene packaging. In this thesis project, there will be a thorough study of the characteristics of fungal mycelium and how it can be combined with other products to create biodegradable packaging. Polystyrene packaging is unsustainable and poses threats to human health and our natural environments. It is pivotal that alternative packaging options for polystyrene are researched and tested to prove reliability as packaging systems develop. Research questions answered in this thesis include: Can fungal mycelium be a biodegradable replacement for plastic/polystyrene packaging at a large scale? What is …

What Are The Impacts Of Plastic Pollution On Sea Turtles And How Can We Prevent Them?, Stacey Safarik

Department of Environmental Studies: Undergraduate Student Theses

This paper will address the impacts of plastic pollution on sea turtles and how humans can combat the issue. Sea turtles are often harmed due to human activities because the ocean is used as a dumping ground for trash, and humans need to take it upon themselves to change this. The purpose of this project was to learn how plastic can impact the lives of sea turtles, and to find out if there are prevention efforts in place. A thematic literature review was conducted in order to find all of the data. Once all of the data was compiled, it …

The Effects Of Phosphate On The Metamorphosis Of Larval Western Barred Tiger Salamanders (Ambystoma Mavortium), Alexis Jean Polivanov

Department of Environmental Studies: Undergraduate Student Theses

This investigation will collect data to assist in determining if elevated aquatic phosphate levels affects the metamorphosis rate of larval western barred tiger salamanders (Ambystoma mavortium). Monoammonium phosphate fertilizers are being used on crops in Nebraska (NDA, 2017). This area lines up with the area the western barred tiger salamanders are disappearing from (Damme, 2018). Monoammonium phosphate is made up of nitrogen and phosphate. There have been several studies showing how nitrogen is harmful to amphibians such as this salamander (Griffis-Kyle, 2007) (Griffis-Kyle & Richtie, 2007), but there have not been many showing how phosphate affects amphibian’s metamorphosis …