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Effects Of Chemical Variation On Competition And Insect Communities Across Solidago Altissima Genotypes, Bryan Scott Foster Ii

Masters Theses

Individuals within a plant species can differ greatly from one another, especially regarding the range of chemical compounds produced. However, the functions of many of these chemicals are unknown, but likely include defenses against herbivores, attractants for pollinators and seed dispersers, as well as mechanisms for resource competition. To assess the effects of foliar chemical composition on interspecific plant competition and insect communities, I conducted a common garden and greenhouse experiment using 24 genotypes of the allelopathic species Solidago altissima for which the foliar chemistry had been characterized. Using these data and chemical profiles of S. altissima, I linked …

Is Context Dependency Imperative To Understanding The Impacts Of Invasive Plants?, Brendan B. Haile

Masters Theses

Introduced exotic species have a tendency to become invasive and impact local biological communities. Invasions often impact community attributes such as cover and species richness, but these factors may also regulate patterns of invasion. In such cases, impacts may be dependent on the invasion context. We used data from the Buell-Small Succession Study, a long-term permanent plot study in the piedmont region of New Jersey, to document context dependency in invasion. To do this, we analyzed the factors that affected the colonization and growth of four invasive species, Alliaria petiolata, Lonicera japonica, Microstegium vimineum and Rosa multiflora, as well …

Genetic And Cellular Models For The Study Of Ptk2b And Adam10 In Adaptive Thermogenesis And Metabolism, Jared S. Farrar

Theses and Dissertations

In a cellular model, we demonstrate that the non-receptor protein tyrosine kinase 2 beta (PTK2B) plays a critical role in mouse cultured beige adipocyte differentiation. CRISPR/Cas9-mediated knock-out of Ptk2b results in non-differentiating white adipocytes and differentiated beige adipocytes with significantly reduced thermogenic gene and protein expression, enlarged lipid droplet size, and altered mitochondrial respiration. Together, these data in a cell culture system provide evidence for a role of PTK2B in the differentiation of mouse beige adipocytes.

In the process of developing a new mouse model utilizing the adipocyte selective Adipoq-Cre transgenic mouse, strong genetic linkage between a gene …

Mass Spectrometry Based Lipidomic Investigation Of Staphylococcus Aureus Lipid Metabolism And Characterization Of Lipase A, Naren Gajenthra Kumar

Theses and Dissertations

Staphylococcus aureus produces an arsenal of virulence factors and is adept at colonizing multiple hosts by employing specific processes to persist at different sites of infection. Infections caused by this pathogen range from self-limiting infections of the skin like folliculitis to life threatening and chronic conditions like infective endocarditis, non-healing diabetic foot ulcers and atopic dermatitis. As the field continues to expand its understanding of the mechanisms by which this pathogen rapidly develops resistance to new antibiotics, the underlying mechanisms that facilitates niche adaptation and host colonization among healthy individuals remain elusive.

Bacterial and host lipid metabolism presents and area …

Caffeine Use And Associations With Sleep In Adolescents With And Without Attention-Deficit/Hyperactivity Disorder (Adhd), Caroline N. Cusick

Theses and Dissertations

The objective of this study was to compare caffeine consumption in the morning, afternoon, and evening in adolescents with and without Attention Deficit/Hyperactivity Disorder (ADHD) and examine associations with sleep functioning. Participants were 302 adolescents (ages 12-14) with (n=140) and without (n=162) ADHD. Adolescents wore actigraph watches to assess total sleep time and wake after sleep onset and reported on their sleep-wake problems and the number of caffeinated beverages consumed per day in the morning, afternoon, and evening. Parents reported on adolescents’ difficulties initiating and maintaining sleep. Chi-square analyses, odds ratios, and path analyses were conducted. Analyses controlled for sex, …

The Effects Of Exosomes On The Regeneration Of Rat Calvaria, Jill T. Beitz

Theses and Dissertations

Periodontal disease is a bacterial induced chronic inflammatory condition that occurs in a susceptible host, affecting the teeth, gingiva, periodontal ligament, and alveolar bone. The resultant pathological bone defects often require surgical intervention, with the ultimate goal being regeneration of the periodontium through the application of grafting materials and biologics. Mesenchymal stem cells (MSCs) show great potential in regenerative medicine for their ability to enhance cell proliferation and differentiation, increase angiogenesis, and modulate the immune response. Stem cell-derived by-products, known as exosomes are a specific class of lipid-membrane-bound extracellular vesicles that are proliferative and chemotactic, are able to inhibit cytokine …

Characterization Of The Dyrk1a Protein-Protein Interaction Network, Varsha Ananthapadmanabhan

Theses and Dissertations

Human Dual specificity tyrosine (Y)-Regulated Kinase 1A (DYRK1A) is a protein kinase encoded by a dosage-dependent gene. An extra copy of DYRK1A contributes to Down syndrome (DS) pathogenesis while loss of one allele causes severe mental retardation and autism. DYRK1A is involved in phosphorylation of several proteins that regulate cell cycle control and tumor suppression. However, the function and regulation of this kinase is not well understood and current knowledge does not fully explain dosage-dependent function of this important kinase. Our previous proteomic studies identified several novel DYRK1A interacting proteins including RNF169, FAM117B, TROAP, LZTS1, LZTS2 and DCAF7. In this …

Extracellular Matrix Nanoparticles Effects On The Lung In Vivo, Brittaney E. Ritchie

Theses and Dissertations

Acute respiratory distress syndrome (ARDS) is a life-threatening condition that causes diffuse alveolar damage and a loss of the extracellular matrix (ECM). This leads to pulmonary edema and lung function deterioration. Our lab has created decellularized porcine lung, electrosprayed ECM nanoparticles that have been previously shown to have pro-regenerative capabilities in vitro.

In this study, the ECM nanoparticle effects on young murine lungs were tested in vivo. An ECM nanoparticle suspension, previously used for the in vitro studies, was aerosolized intratracheally into the lungs using a microsprayer. 24 hours later, the lung mechanics, bronchoalveolar lavage fluid, and histology …

Negative Urgency As A Risk Factor For Intimate Partner Aggression: The Untold Story Of Maladaptive Interpersonal Emotion Regulation, Alexandra M. Martelli

Theses and Dissertations

Interpersonal conflict frequently occurs in daily life, especially within intimate relationship contexts. The strategies people use to respond to conflict can either bring couples together or push them further apart. Furthermore, impulsive traits, such as negative urgency, leave individuals vulnerable to conflict. Individuals high in negative urgency have difficulty regulating their emotional reactions to negative experiences, often failing to employ adaptive interpersonal emotion regulation (IER) strategies. No research to date has examined the link between negative urgency and the use of negative IER. Fifty-four couples completed self-report measures of negative urgency, effective interpersonal regulation of negative affect, and intimate partner …

The Correlation Between Maternal Postpartum Depression And Child Psychopathology, T'Keyah I. Vaughan

Theses and Dissertations

Background: Postpartum depression (PPD) is a phenomenon that affects nearly 10-15% of pregnancies in the US. It is characterized by depressed mood or anhedonia and lasting for more than 2 weeks. PPD changes how moms interact with family members and child-rearing behavior. Depression is a phenomenon that is also known to affect the psychopathology of children. However, the specifics of how postpartum depression impacts children remains controversial. Many studies do not control for major depressive disorder which makes it difficult to disentangle the impact depression has within the first year of life. Furthermore, other PPD risk factors may be confounding …

The Immediate And Persistent Effects Of Binge Ethanol Exposure On Myelin Protein Expression In Dba/2j Mice, Aminatta Z. Tejan-Kamara

Theses and Dissertations

Binge drinking is the most lethal of drinking patterns, responsible for 77% of all U.S. alcohol misuse costs. Adolescents have increased sensitivity to ethanol’s rewarding properties and decreased sensitivity to ethanol’s aversive effects likely allowing for their increased binge drinking, as compared to adults. Ethanol consumption during adolescent neurodevelopment can lead to immediate and lasting neurobiological-consequences. We have previously shown that adolescent intermittent binge ethanol exposure decreases myelin-related gene expression in DBA/2J mice. Here, we hypothesize intermittent binge ethanol exposure in adolescent DBA/2J mice will decrease myelin protein expression in the prefrontal cortex (PFC) immediately following binge ethanol and this …

The Intersection Of Social Stress, Mental Well-Being, And Sexual Health Among Black Women In Emerging Adulthood, Brandi Galloway

Theses and Dissertations

With an estimated 37,000 new HIV infections each year, HIV continues to be a major public health concern. HIV affects some populations more than others. Young Black women, in particular, are disproportionately affected by HIV. While being a woman does not typically increase a person’s HIV risk, being Black and being a woman does.

Data indicate that individual-level factors do not fully address the differences in HIV and STIs between Black emerging adult women and their White counterparts. Thus, it is critical to better understand contextual factors such as social stress and mental-wellbeing which might better account for these disparities. …

Biopsychosocial Variables Associated With The Development Of Chronic Low Back Pain In Healthcare Workers, Jena Pauli

Theses and Dissertations

ABSTRACT

Purpose: Worldwide the incidence and prevalence of acute low back injury with pain (ALBIP) is increasing in healthcare workers (HCW). Approximately 27% of ALBIP result in chronic low back pain (CLBP). The primary aim of this study was to identify biopsychosocial factors that contribute to the development of CLBP. A secondary aim was to examine the predictive value of reliable and valid screening instruments to identify individuals at highest risk for CLBP.

Significance: Low back pain is the second most commonly reported pain condition in the United States, one of the leading causes of sick leave and …

Pharmacological Characterization Of Novel Low Efficacy Mor Modulators: Implications For Opioid Use Disorder Treatment, Abdulmajeed Jali

Theses and Dissertations

Opioid use disorder (OUD) is a major public health concern in the Unites States, resulting in an estimated 128 overdose deaths per day. Although presently approved OUD pharmacotherapies are partially effective, they are accompanied by undesirable side effects or low compliance. m-Opioid receptor (MOR) agonists (methadone and buprenorphine) are associated with physical dependence, abuse liability and respiratory depression, whereas the MOR antagonist naltrexone is associated with withdrawal precipitation, dysphoria and low patient compliance. Therefore, development of novel medications with fewer side effects and higher compliance than current OUD pharmacotherapies is needed. The objective of this dissertation was to pharmacologically characterize …

The Human Intermediate Prolactin Receptor: A Breast Cancer Proto-Oncogene, Jacqueline M. Grible

Theses and Dissertations

The hormone prolactin (PRL) and its receptor (hPRLr) are significantly involved in breast cancer pathogenesis. The intermediate hPRLr (hPRLrI) is an alternatively-spliced isoform, capable of stimulating cellular viability and proliferation. An analogous truncated mouse PRLr (mPRLr) was recently found to be oncogenic when co-expressed with wild-type mPRLr. hPRLrI co-expression with full-length hPRLr (hPRLrL) in MCF10AT cells resulted in robust in vivo and in vitro transformation, while hPRLrI knock-down in MCF7 cells significantly decreased in vitro malignant potential. hPRLrL+I heterodimers displayed greater stability than hPRLrL homodimers, and while being capable of activating Jak2, Ras, and MAPK, they were unable to induce …

“It’S Supposed To Be Super Easy And It’S Not”: Black And Latinx Student Experiences In The Doctor Of Physical Therapy Admissions Process, Melissa Yeung

Theses and Dissertations

Data provided by the Physical Therapy Central Application System (PTCAS) suggests that despite the large numbers of applicants to Doctor of Physical Therapy (DPT) programs on an annual basis, a gap remains between the number of White and Black and Latinx applicants who are offered admission to DPT programs. Efforts made by DPT programs to increase the diversity of their incoming cohorts include the use of holistic review processes and enrichment programs to recruit more diverse classes. However, research suggests that the undergraduate experiences of students of color have an impact on their aspirations to graduate and professional schools, and …

The Role Of Manganese In Streptococcus Sanguinis, Tanya M. Puccio

Theses and Dissertations

Streptococcus sanguinis is primarily associated with oral health as a commensal bacterium. As an opportunistic pathogen, S. sanguinis is capable of colonizing heart valve vegetations, leading to the disease infective endocarditis. Previous studies from our lab have identified the high-affinity manganese transporter SsaACB as important for endocarditis virulence. The impact that manganese depletion has on S. sanguinis had never been evaluated and a secondary manganese transporter has not been identified. Thus, we employed the use of a fermentor to control large-scale growth over time and depleted manganese in an ΔssaACB mutant using a metal chelator, EDTA. The changes in …

Development Of An Anatomically And Electrically Conductive Brain Phantom For Transcranial Magnetic Stimulation, Hamzah A. Magsood

Theses and Dissertations

Transcranial Magnetic Stimulation (TMS) is a non-invasive technique for diagnostics, prognostic, and treatments of various neurological diseases. However, the lack of anatomically realistic brain phantoms has made the experimental verification of stimulation strength in the form of induced electric fields/voltages in the brain tissues an impediment to developing new TMS coils, stimulators, and treatment protocols. There are significant technological, safety, and ethical limitations to test the potential TMS treatment procedures or develop enhancements and refine them on humans or animals. This work aims to bridge the gap by introducing and developing an innovative manufacturing and fabrications process to produce a …

Assessing The Role Of Planar Cell Polarity Protein Ptk7 In Axon Pathfinding Of Spinal Commissural Neurons, Joseph Paul Miano

Theses and Dissertations

Axon pathfinding is a fundamental step that establishes the vast networks of the nervous system. Understanding this process is important because disruptions to these neural networks can impair quality of life. During axon pathfinding, an axon’s growth cone comes in contact with a variety of secreted chemotropic signaling cues that control growth cone behavior through activation of signal transduction pathways which cause changes to the cytoskeleton. One such pathway is the Planar Cell Polarity (PCP) signal transduction pathway that responds to gradients of non-canonical Wnt ligands. PCP has a well-known role in axon pathfinding, including axon pathfinding in the spinal …

Development Of A Single Use Device Intended To Wash Blood Clot Debris From A Stent Retriever In The Operating Room, Shane Diller

Theses and Dissertations

Ischemic stroke is a life-threatening condition that requires immediate surgical intervention to remove the blood clot that is lodged in the blood vessels of the brain. Mechanical thrombectomy, performed by an endovascular surgeon, involves inserting a stent retriever, which deploys distally and attempts to capture the clot when dragged backwards. A complication prevalent in 50% - 67% of cases is that the blood clot is only partially removed and clings to the mesh framework of the stent. The operating surgeon must either clean the debris from the stent retriever, to be reinserted or throw the stent retriever away and open …

Changing Landscapes: Perspectives Of Young African American Women Of Population-Wide Testing For Hereditary Breast Cancer Mutations, Alesha Henderson

Theses and Dissertations

Young African American women continue to die from breast cancer at higher rates than White women. In an effort to promote breast cancer prevention, population-wide testing (PWT) for all women in the U.S. starting at age 30 is a potential future initiative that is garnering a lot of attention. However, African American women currently utilize genetic testing for hereditary breast cancer at lower rates than White women. Therefore, if young African American women are not prioritized in a future roll out of PWT, then existing breast cancer and genetic testing disparities will likely continue to widen. The purpose of this …

Belowground Characteristics Of Dominant Coastal Dune Grasses And Potential Community-Level Effects On Coastal Erosion, Shannon L. Walker

Theses and Dissertations

Natural dunes arise out of complex relationships between ecological, hydrological, and geological processes and are important for reducing erosion along coastlines. Aboveground structures of coastal dune grasses are known to impact erosional dynamics, and recent studies have shown that belowground structures—such as roots, rhizomes, and belowground stems— may be important in erosional resistance. My objectives were to 1) characterize above- and belowground characteristics of prominent dune grasses and 2) combine these data with functional group abundances and distribution to evaluate community effects on two adjacent locations of distinctive morphology and erosional characteristics and their response to storm disturbance. Whole plant …

Development Of Supported Pd-Based Catalysts For Carbon-Carbon Cross-Coupling And Oxidation Reactions, Mrinmoy Das

Theses and Dissertations

Heterogeneous catalysts are a crucial part of chemistry used for various chemical synthesis stages, pharmaceutical, and environmental applications. The use of nanoparticles or rather nanocatalysts enhances the heterogeneous catalytic applications. Both chemical and physical methods can be used to synthesize nanocatalysts. Chemical synthesis methods require comparatively fewer instruments and can easily synthesize a large amount of nanocatalysts. Nevertheless, chemical synthesis methods require reducing agents and solvents that can be toxic or harmful materials. They often need harsh reaction conditions as well, like high temperature and pressure. On the other hand, physical synthesis methods often require complex instrument setup, but their …

Ndrg1 And Myelin-Related Disease: Alcoholism And Chemotherapy-Induced Neuropathy, Guy Harris

Theses and Dissertations

Alcohol use disorder (AUD) is a prevalent neuropsychiatric disease with profound health, social, and economic consequences. With an estimated 50% heritability, identifying genes that engender risk and contribute to the underlying neurobiological mechanisms represents an important first step in developing effective treatments. Gene expression studies are an important source of candidate genes for studying AUD, providing windows into the molecular machinery engaged by the brain in response to ethanol. Our laboratory has implicated N-myc down-regulated gene 1 (Ndrg1) as a potential candidate gene that modulates ethanol-induced changes in myelin-related gene expression and acute sensitivity to ethanol. Analysis of …

Characterizing A20 Function In Periodontal Tissue Homeostasis, Erin Mooney

Theses and Dissertations

The regulation of inflammatory signaling pathways is critical for proper innate immune responses to pathogens as well as the prevention of chronic inflammation. Ubiquitination, a covalent posttranslational modification, has been identified as a key regulator of multiple signaling cascades, including nuclear factor (NF)-κB. A20 (also known as TNF alpha induced protein 3 or TNFAIP3) has emerged as critical ubiquitin-editing enzyme that functions as an endogenous regulator of inflammation through termination of NF-κB activation as part of a negative feedback loop. Through its function in a plethora of biological processes, aberrant expression and/or function of A20 has been implicated in gastrointestinal, …

Transcriptomic Profiling Of Postmortem Prefrontal Cortex And Nucleus Accumbens From Chronic Alcohol Abusers., Eric S. Vornholt

Theses and Dissertations

Alcohol use disorder (AUD) is a debilitating psychiatric illness that develops from a combination of genetic and environmental factors. While it is well documented that AUD is heritable, the shift from recreational alcohol use to abuse/dependence is poorly understood. In this dissertation, using postmortem brain tissue from individuals with alcohol dependence (AD), we profiled the genome-wide expression of circular RNA (circRNA), microRNA (miRNA), and messenger RNA (mRNA) to better understand the impact of gene expression on the development of AUD. To achieve this, we performed two independent studies that explore transcriptome differences between AD cases and controls. The first of …

Culturally-Relevant Factors That Influence Healthy Eating Among African American College Students, Danyel Smith

Theses and Dissertations

Obesity is a national epidemic in the US, disproportionately affecting African Americans, such that the obesity prevalence in African Americans (49.6%) surpassed the national prevalence (42.4%) in 2018. Those same disparities exist at the collegiate level, such that 27.4% of a sample of African Americans college students had obesity, surpassing the national prevalence of obesity (12.1%) in 2018. Diet, an important driver of obesity, is influenced by several psychological, social, and environmental factors. However, cultural factors influencing diet are understudied among African American college students. The overarching goal of the current study was to identify culturally-relevant factors that promote healthy …

The Origin And Evolution Of Flower Characters In Angiosperms With A Case Study On The Genetic Basis Of Zygomorphic Corolla Development In Bee And Hummingbird Pollinated Species Of Schizanthus (Solanaceae), Ghadeer Bukhari

Theses and Dissertations

This Ph.D. dissertation investigates two main projects separated into two chapters, one for each. The first chapter explores the evolution of flowering plants (angiosperms), a macro-scale study. The second project is on a micro-scale that focus on exploring the genes that regulate the similarities and the differences of flower morphology reflecting their different pollination syndromes in two species of Schizanthus (Solanaceae), i.e., S. pinnatus, a bee-pollinated flower, and S. grahamii, a hummingbird- pollinated flower.

In the first chapter, I used floral diagrams to study the origin and the evolution of floral traits in angiosperms, a new set of morphological data …

Discerning The Spectroscopic Properties And Chemical Reactivity Of Bioinspired Metallocomplexes, Ashley K. Forney

Theses and Dissertations

In Nature, metalloenzymes catalyze a variety of thermodynamically demanding reactions with remarkable performance under mild, physiological conditions. This innate ability is a driving force for the development of nature inspired, synthetic analogues that are robust and can catalyze a variety of organic transformations. This work involves the successful syntheses of four coordinate, bioinspired complexes, [M^II(n-DOB)] (M = Ni, Co, and Cu; n = 12 and 13), which have been characterized by NMR, ESI-MS, UV/Vis, EPR, and CV. Spectroscopic evidence presents the generation of reactive metal-(di)oxygen species that display amphoteric reaction behavior, making these rarities among inorganic oxidants. These …

Profiles Of Adjustment Among Children In Institutional Care In Ghana: Predictors Of Positive Functioning, Anna W. Wright

Theses and Dissertations

Worldwide, approximately 2-8 million children reside in institutional care, which can lead to developmental setbacks. Given the need for institutional care persists, it is important to continue investigating which improvements can be made to provide optimal care. The current mixed-methods study included participants from Accra, Ghana. Study 1 involved cluster analyses to determine patterns of adjustment for 100 children in institutional care (CIC) (M age = 13.31 years, SD = 3.14; 40% female) and 100 children in families (CIF) (M age = 13.37 years, SD = 3.08; 40% female) across several domains. ANOVA was used to evaluate whether …