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From Garlic To Acupuncture: Cultural Models Of Covid-19 In Traditional Chinese Medicine, Molly Eaton

Honors College Theses

Ever since I studied Traditional Chinese Medicine (TCM) in Yunnan, China, I have been curious about it. The cultural and historical aspect of TCM combined with the medical perspective provides a unique concept that is vastly different from Western Medicine (WM). TCM has been practiced for thousands of years in China and surrounding areas. It has seen the rise and fall of kingdoms. It has fought against all types of injuries and illnesses. With the curiosity of TCM combined with the daunting COVID-19, I opted to research how people 3 practice TCM during COVID-19. This research project seeks to understand …

Coral Bleaching: A Global Analysis Of Bright Spots, Shannon Elizabeth Sully

Theses and Dissertations

Thermal-stress events associated with climate change cause coral bleaching and mortality that threatens coral reefs globally. Yet coral bleaching and coral cover patterns vary spatially and temporally, and few studies have identified bright spots where corals have the potential to survive through climate change. This study synthesizes over 10,000 field observations globally of coral bleaching and coral cover from 1997to 2017 and uses a suite of environmental and temperature metrics to analyze coral bleaching and coral cover patterns. The objectives of this dissertation are to(1) identify the environmental variables that constitute bright spots for coral reefs, and (2) identify where …

3d Printed Microporous Collagen Constructs Using The Freeze-Fresh Methodology, Thais Sousa Senzano

Theses and Dissertations

Scaffold microporosity is known to play a critical role in governing cellular response including cell migration, proliferation, and tissue-specific differentiation. While fabrication approaches such as solvent leaching and freeze-casting are commonly used for the generation of microporous biomaterial scaffolds, these methods provide little control over scaffold geometry and creation of complex tissue structures. Extrusion-based 3D printing, an additive manufacturing method, is a highly versatile layer-by-layer printing approach that allows for the fabrication of easily customizable 3D scaffolds with complex architecture using a vast selection of polymeric bioinks. These 3D printed constructs are porous at the macroscale, but achieving microporosity (i.e., < 100 μm) in printed constructs is challenging due to the sub-optimal resolution of the extrusion-based printing method. A recent study using alginate inks introduced a new fabrication technique termed Freeze-FRESH(FF)that combines extrusion printing and freeze-casting approaches to generate 3D constructs with hierarchical microporosity. However, the porosity of the resultant alginate constructs was comparable despite changing the freezing temperature. In the current study, the FF method was modified to print collagen constructs with greater control of microporous architecture. Highly concentrated collagen type I ink was used to 3D print collagen constructs using the freeform reversible embedding of suspended hydrogels (FRESH)approach. Modification of the FF technique entailed melting of the FRESH bath post printing via incubation at 37 °C followed by freezing and lyophilization to allow for collagen gelation and better heat transport during the freezing process. The effect of freezing temperature on micropore size, swelling degree, degradation, and mechanical properties of printed constructs was assessed. Finite element (FE) models were generated to predict the mechanical properties of microporous scaffolds. In addition, the effect of microporosity on Saos-2 cell morphology, proliferation, infiltration, and ALP activity was evaluated. Results from the study showed that freezing temperature effectively modulated micropore size and that constructs with larger micropore size were more stable. Microporosity had no effect on swelling ratio yet caused a decrease in mechanical properties of collagen constructs; FE models confirmed experimental results. Cell metabolic activity and infiltration was enhanced in microporous constructs with larger pore size, yet there was no effect on cell morphology, and ALP activity. In conclusion, the modified FF technique can be effectively used to fabricate hierarchically porous 3D collagen constructs.

Entry-Level Workplace Competencies Needed By Graduates Of A Community College Agriculture Program: A Midwest Case Study Using The Delphi Technique, Blake Colclasure

Journal of Research in Technical Careers

Community colleges have a strong history of providing vocational education and occupational training to meet changing workforce demands of local industries. In the Midwest, agricultural industries have expressed an expanding need for middle-skilled workers with postsecondary, prebaccalaureate credentials. The 21st century agricultural landscape has changed as a result of the need for efficiency and sustainability, and resulting emergent agricultural technologies. Community colleges will be integral to establishing a qualified agriculture workforce for entry-level, middle-skilled positions. This study utilized a modified Delphi approach to identify entry-level workplace competencies needed by graduates of a community college agriculture program. Delphi panel experts represented …

Dispositions Of Emotional Intelligence In School Principals, Efrain Martinez

Dissertations

School principals address problems that extend way beyond the classroom. This qualitative study is about dispositions of emotional intelligence in school principals. The participants interviewed were novice school principals or principal coaches. The results showed that principals tend to address the needs of others more than their own. Strategies are shared on how to improve emotional intelligence. Additionally, the study provides recommendations that school districts can implement to ensure the success of school principals.

Electromagnetic Interference Estimation Via Conditional Neural Processing, Edgar E. Gomez

Theses and Dissertations

The goal of this thesis is to determine the efficacy of employing Machine Learning (ML) to solve Joint Urgent Operational Need (JUON) CC-0575, which aims to develop a Common Operating Picture (COP) of the Global Positioning System (GPS) Electromagnetic Interference (EMI) environment. With the growing popularity of Artificial Neural Networks (ANNs), ML solutions are quickly gaining traction in businesses, academia and government. This in turn allows for problem solutions that were previously inconceivable using the classical programming paradigm. This thesis proposes a method to develop a COP of the battlefield via ANN ingestion of multiple-source signals and sensors. We conduct …

A Collaborative Effort To Establish A Convalescent Plasma Program In South Florida, Roberto L. Roman Laporte, Edwina Brathwaite

Nursing & Health Sciences Research Journal

On March 11, 2020, Miami-Dade County had its first diagnosed case of COVID-19 and quickly became the top hotspot of COVID-19 cases in Florida. Hospitals were challenged to find effective treatments to combat the virus. A collaborative effort between Doctors Hospital and Boca Raton Regional Hospital established a robust convalescent plasma program for critically ill patients diagnosed with COVID-19 within Baptist Health South Florida.

Efficacy Of The Nutritional Supplement, Evenflo, In The Management Of Sickle Cell Disease: A Randomized Controlled Trial, Richard Muga, Arthur Ajwang, James Ouma, Josephine Ojigo, Juliana Otieno, Peter Okoth, Caroline Wafula, Sophia Ajwang, David Ogolla, Abraham Hollist, James Adefisoye

Nursing & Health Sciences Research Journal

Background: In this study, we investigated if a combination of the nutraceutical supplement, EvenFlo and folic acid will be superior to the standard stand-alone use of folic acid.

Methods: We conducted a randomized double-blind, active-controlled, clinical trial. A total of 70 subjects with SCD ages 5-12 years were enrolled into the study with 35 in the intervention group and 35 in the control group; 61 completed the trial (32 from the intervention group and 29 from the control group).

Results: Participants in the intervention group were significantly less likely to experience crises compared to subjects in the control group. None …

A New Paper-Based Microfluidic Device For Improved Detection Of Nitrate In Water, Amer Charbaji, Hojat Heidari-Bafroui, Constantine Anagnostopoulos, Mohammad Faghri

Mechanical, Industrial & Systems Engineering Faculty Publications

In this paper, we report a simple and inexpensive paper-based microfluidic device for detecting nitrate in water. This device incorporates two recent developments in paper-based technology suitable for nitrate detection and has an optimized microfluidic design. The first technical advancement employed is an innovative fibrous composite material made up of cotton fibers and zinc microparticles that can be incorporated in paper-based devices and results in better nitrate reduction. The second is a detection zone with an immobilized reagent that allows the passage of a larger sample volume. Different acids were tested—citric and phosphoric acids gave better results than hydrochloric acid …

Comprehensive Survey Of The Litter Bacterial Communities In Commercial Turkey Farms, Bishnu Adhikari, Guillermo Tellez-Isaias, Tieshan Jiang, Brian Wooming, Young Min Kwon

Poultry Science Faculty Publications and Presentations

The importance of microbiota in the health and diseases of farm animals has been well-documented for diverse animal species. However, studies on microbiotas in turkey and turkey farms are relatively limited as compared to other farm animal species. In this study, we performed a comprehensive survey of the litter microbiotas in 5 commercial turkey farms in the Northwest Arkansas (H, M, V, K, and R farms) including one farm with positive incidence of cellulitis (R farm). Altogether 246 boot swabs were used for 16S rRNA gene profiling of bacterial communities. At phylum level, 11 major bacterial phyla (≥0.01%) were recovered. …

Spiritually Guided And Bridging The Gap: An Inquisitive Analysis On The Correlation Of Pastoral Counseling And Mental Health, Cynthia E. Taylor

Doctoral Dissertations and Projects

This research study evaluates the outcomes of pastoral counseling on mental health within a predominantly African American congregation in the southeastern United States. The research study is intended to build a further understanding of the pastor’s role in counseling individuals with mental health conditions, given individuals seek counseling as a treatment option. To evaluate those perceptions, the researcher analyzed data collected from a survey. The outcomes from this qualitative data study suggest that pastoral counseling alone is ineffective given several factors such as individual failure to seek counseling, no suggested mental-health related issues within the sampled population, and ethnicity barriers. …

The Impact Of Social Media Use On Social Comparison And Body Images Of Young Adult Males, Tresa Lynn Hildreth

Doctoral Dissertations and Projects

With the rise of social networking sites, young people are utilizing social platforms as an on-going avenue for comparison, specifically with regard to body image. Although issues of body image are often attributed to females, males struggle to live up to societal ideals of what a man should look like. This study highlights the body image comparisons that exist with young men and provides insight into the detrimental impact and consequences these comparisons can have on young men as they grow into adulthood. The purpose of this study was to investigate the relationship between social media use and body image …

A Bicultural Service Member/Civilian Identity And Motivations-Based Assessment Of Moral Injury In U.S. Military And Veteran Populations From America’S Longest Wars, Jamie Allison Mcnally

Doctoral Dissertations and Projects

Experiences in war are often traumatic in nature. Over the past thirty years, research has resulted in increased understanding of these experiences and the unique trajectory that values-based, as opposed to fear-based, traumas can cause. This study investigates these values-based traumas and the mental health outcomes that may result in a syndrome known as moral injury. The current research examined the relationship between potentially morally injurious event exposure and mental health symptomology. Event exposure was conceptualized both as objective occurrences and as the subjective experience of events, with notable distinctions in significance of outcomes. After attempting to replicate previous research …

An Effective Strategy Of Family Ministry In Korean Church To Build A Biblical Family, Ikjae Jin

Doctoral Dissertations and Projects

The purpose of this project is to assist churches in building a biblical family and developing an effective strategy for family ministry programs. Additionally, it will address the problems and difficulties of Korean families. The study will be based on Korean churches in South Korea. A study of what churches are doing to assist these families will be conducted. This project will suggest how to build a biblical family in Korean churches. This research will survey at least 50 Korean pastors living in Korea and will conduct research on existing family ministry programs in Korean churches based on biblical and …

Loving The Unloveable: A Biblical Approach For The Local Church To Homosexuality In A Morally Declining Society, Robert Jonathan Archer

Doctoral Dissertations and Projects

This thesis will strive to prove that local churches worldwide have been highly ineffective at providing true recovery for parishioners that struggle with homosexuality often ostracizing them as a diseased product rather than a creation of the Father. The goal is to help the church discover, assess, and address the sin, healing, and recovery for homosexuals in their ministry in a healthy biblical manner that promotes freedom in Christ and true recovery. This will allow the local church to partake in the healing process of broken parishioners promoting evangelism and discipleship that will in turn extend into the communities of …

A Correlational Study Of Perceptions Of Favoritism, Job Satisfaction, And Job Attendance, Edwanda Landrum Jackson

Doctoral Dissertations and Projects

Researchers posit that teachers’ excessive absences may be caused by a lack of commitment or dissatisfaction with their jobs due to school environments and relationships, and a lack of commitment can ultimately influence student achievement. This correlational study sought to determine any relationship between teachers’ absenteeism and job satisfaction as mitigated by perceptions of favoritism. The participants included 38 teachers among 24 primary and secondary schools in a medium-size, rural, school district. This correlational, quantitative research study utilized instruments titled Job Satisfaction Survey and the Nepotism, Favoritism, Cronyism questionnaire to examine the perceptions of job satisfaction and favoritism among 38 …

A Phenomenological Study Of Traditional Public High School Graduates' Experiences While Taking Their First Online Course In College, David B. Warner

Doctoral Dissertations and Projects

The purpose of this phenomenological study was to understand the shared lived experiences of traditional high school graduates while taking an online course for the first time. Many colleges and universities provide online courses as part of their regular instructional program. Some colleges and universities require students to take an online course early in their post-secondary education. However, there are a limited number of k-12 public-school districts in the United States that provide opportunities for high school students to take online courses as part of the regular curriculum. This phenomenological study was designed to investigate the perceptions of traditional high …

Self-Induced Moral Injuries, Sexual Shame, And Well-Being: The Moderating Role Of Self-Forgiveness, Teresa Lynn Phillips-Harris

Doctoral Dissertations and Projects

Self-induced moral injuries caused by compulsive sexual behavior can result in experiences of sexual shame. Shame is a pervasive experience of unworthiness, and it can become a significant obstacle to life and relationship satisfaction because it leads to negative cognitions and isolating behaviors. Self-induced moral injuries occur when a person's wrongdoing challenges their worldview and spiritual beliefs. Moral injuries impact the individual's affect, cognition, and behavioral responses to morally traumatic events. Individuals who morally injure themselves and others struggle to take responsibility, make cognitive and behavioral changes, and forgive themselves. Several research studies have examined the impact of shame on …

Student Success Of Online Vs. In-Person Biology Courses At Virginia Community Colleges, Jennifer Claire Scott

Doctoral Dissertations and Projects

Community colleges have a significant role in preparing students for STEM-related careers through certificates, degrees, and transfers to four-year institutions. In addition, online education is a growing mode of higher education, particularly for community college students. However, community college and online students are both at a high risk of attrition and show a lower success rate for degree completion. The purpose of this study was to identify differences in attrition and success between students in online and in-person biology courses at Virginia community colleges. Also, this study addressed downstream effects of online education by examining course completion of second-semester biology …

My Spouse Is Amazing Like Me: The Association Between Homogamy For Narcissism And Relationship Satisfaction, Kerry Anne Marsh

Doctoral Dissertations and Projects

This study’s purpose was to better understand how narcissistic admiration and rivalry relate to relationship satisfaction and to investigate the satisfaction found in homogamous versus heterogamous narcissistic relationships. The study explored relationship satisfaction of married participants (N = 764), as moderated by their partner’s perceived level and subtype of narcissism. It also examined direct correlations between participant narcissistic admiration and rivalry and relationship satisfaction, as well as correlations between participant and perceived partner narcissism scores. An exploratory study about how relationship length moderates the association between narcissism and relationship satisfaction was also conducted. This quantitative study featured a cross-sectional online …

Opportunities And Challenges Of Geospatial Analysis For Promoting Urban Livability In The Era Of Big Data And Machine Learning, Anna Kovacs-Györi, Alina Ristea, Clemens Havas, Michael Mehaffy, Hartwig H. Hochmair, Bernd Resch, Levente Juhasz, Arthur Lehner, Laxmi Ramasubramanian, Thomas Blaschke

Faculty Research, Scholarly, and Creative Activity

Urban systems involve a multitude of closely intertwined components, which are more measurable than before due to new sensors, data collection, and spatio-temporal analysis methods. Turning these data into knowledge to facilitate planning efforts in addressing current challenges of urban complex systems requires advanced interdisciplinary analysis methods, such as urban informatics or urban data science. Yet, by applying a purely data-driven approach, it is too easy to get lost in the ‘forest’ of data, and to miss the ‘trees’ of successful, livable cities that are the ultimate aim of urban planning. This paper assesses how geospatial data, and urban analysis, …

Gene Products And Processes Contributing To Lanthanide Homeostasis And Methanol Metabolism In Methylorubrum Extorquens Am1, Paula Roszczenko-Jasińska, Huong N. Vu, Gabriel A. Subuyuj, Ralph Valentine Crisostomo, James Cai, Nicholas F. Lien, Erik J. Clippard, Elena M. Ayala, Richard T. Ngo, Fauna Yarza, Justin P. Wingett, Charumathi Raghuraman, Caitlin A. Hoeber, Norma C. Martinez-Gomez, Elizabeth Skovran

Faculty Research, Scholarly, and Creative Activity

Lanthanide elements have been recently recognized as “new life metals” yet much remains unknown regarding lanthanide acquisition and homeostasis. In Methylorubrum extorquens AM1, the periplasmic lanthanide-dependent methanol dehydrogenase XoxF1 produces formaldehyde, which is lethal if allowed to accumulate. This property enabled a transposon mutagenesis study and growth studies to confirm novel gene products required for XoxF1 function. The identified genes encode an MxaD homolog, an ABC-type transporter, an aminopeptidase, a putative homospermidine synthase, and two genes of unknown function annotated as orf6 and orf7. Lanthanide transport and trafficking genes were also identified. Growth and lanthanide uptake were measured using strains …

Inducible Epithelial Resistance Against Acute Viral Pneumonia And Chronic Asthma, Shradha Wali

Dissertations and Theses (Open Access)

Viral pneumonia remains a global health threat despite worldwide vaccination and therapeutic programs. The influenza pandemic of 1918 and SARS-CoV2 pandemic of 2019-2020 are cautionary reminders demanding the need for novel treatment strategies. Moreover, in addition to causing acute disease, respiratory virus infections are often complicated by chronic lung pathologies, such as asthma induction, progression, and exacerbation. We have reported that mice treated with a combination of inhaled Toll-like receptor (TLR) 2/6 and TLR 9 agonists (Pam2-ODN) to stimulate innate immunity are broadly protected against respiratory pathogens, but the mechanisms underlying this protection remain incompletely elucidated. Here, we show in …

Hyperpolarized Carbon-13 Magnetic Resonance Measurements Of Tissue Perfusion And Metabolism, Keith Michel

Dissertations and Theses (Open Access)

Hyperpolarized Magnetic Resonance Imaging (HP MRI) is an emerging modality that enables non-invasive interrogation of cells and tissues with unprecedented biochemical detail. This technology provides rapid imaging measurements of the activity of a small quantity of molecules with a strongly polarized nuclear magnetic moment. This polarization is created in a polarizer separate from the imaging magnet, and decays continuously towards a non-detectable thermal equilibrium once the imaging agent is removed from the polarizer and administered by intravenous injection. Specialized imaging strategies are therefore needed to extract as much information as possible from the HP signal during its limited lifetime.

In …

Subclonal Evolution Of Chronic Lymphocytic Leukemia After Allogeneic T Cell Therapies, Haven Garber

Dissertations and Theses (Open Access)

Subclonal evolution of chronic lymphocytic leukemia after allogeneic T-cell therapies

Haven Garber, MD

Advisory Professor: Jeffrey Molldrem, MD

Intratumoral genetic heterogeneity describes the molecular differences among subclones within a tumor and is a major barrier to effective therapy in many solid and liquid cancers, including chronic lymphocytic leukemia (CLL). Rare, treatment-resistant subclones can expand to compose relapsed disease during tumor evolution. Examination of malignant evolution in the context of specific treatment provides insight into the molecular lesions that mediate therapeutic response and resistance. Both chemotherapy and targeted therapy were shown to precipitate CLL subclonal evolution. We hypothesized that allogeneic T-cell …

Genetic And Clinical Determinants Of Racial/Ethnic Differences In Multiple Myeloma Susceptibility And Outcomes Focusing On Hispanics, Alem Belachew

Dissertations and Theses (Open Access)

Multiple Myeloma (MM) constitutes 10% of diagnosed hematologic malignancies in the US, with over 12,000 deaths recorded each year. Race/ethnicity is a well-known MM risk factor, where individuals of African descent have over 2- to 3-fold increased risk of incidence compared to those of European descent. Additionally, Hispanics are diagnosed approximately three years younger than white American counterparts, for unknown reasons. Differences in clinical phenotype are also present for MM patients by ancestry, including varying rates of common initiation mutations such as IgH translocations and TP53 mutation between patients of European and African descent. Studies have begun to interrogate the …

A Context-Forward In Vivo Functional Genomics Platform For Target Discovery And Establishing Vulnerability Context In Pancreatic Cancer, Johnathon Rose, Johnathon Lynn Rose

Dissertations and Theses (Open Access)

Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive malignancy with a very poor patient prognosis (5-year survival of ≤ 7%). While transcriptional profiling has aided in the classification of this disease into at least two broader subtypes, this alone has so far been insufficient to inform on more nuanced patterns of oncogenic dependency. We hypothesized that a more comprehensive and granular characterization of PDAC disease diversity is required to establish relevant context for targeted therapy. To this end, we sought to establish an integrated platform to: i) more comprehensively characterize differential oncogenic signaling across our tumor models, and ii) establish …

A Pharmacological Strategy Against African Sleeping Sickness, Wahaj Zuberi

Dissertations and Theses (Open Access)

Trypanosoma brucei is a parasitic eukaryote and is the causal agent of the disease known as African sleeping sickness, transmitted via the bite of a tsetse fly. If left untreated, the parasite ultimately crosses the blood-brain eventually leading to death. Currently there are 5 approved drugs used to treat it, each with toxic side effects and effective at specific disease stages. A more effective and less toxic drug candidate is highly sought after. The essential enzyme, dUTPase, is an excellent drug target in the parasite while utilizing S. cerevisiae as a model system.

dUTPase is essential in T. brucei (tbdUTPase), …

P53 Drives A Transcriptional Program That Elicits A Non-Cell-Autonomous Response And Alters Cell State In Vivo, Sydney Moyer

Dissertations and Theses (Open Access)

Cell stress and DNA damage activate the tumor suppressor p53, triggering transcriptional activation of a myriad of target genes. The molecular, morphological, and physiological consequences of this activation remain poorly understood in vivo. We activated a p53 transcriptional program in mice by deletion of Mdm2, a gene which encodes the major p53 inhibitor. By overlaying tissue-specific RNA-sequencing data from pancreas, small intestine, ovary, kidney, and heart with existing p53 ChIP-sequencing, we identified a large repertoire of tissue-specific p53 genes and a common p53 transcriptional signature of seven genes which included Mdm2 but not p21. Global p53 activation …

Development Of Computational To Ols To Target Microrna, Luo Song

Dissertations and Theses (Open Access)

MicroRNAs (a.k.a, miRNAs) play an important role in disease development. However, few of their structures have been determined and structure-based computational methods remain challenging in accurately predicting their interactions with small molecules. To address this issue, my thesis is to develop integrated approaches to screening for novel inhibitors by targeting specific structure motifs in miRNAs. The project starts with implementing a tool to find potential miRNA targets with desired motifs. I combined both sequence information of miRNAs and known RNA structure data from Protein Data Bank (PDB) to predict the miRNA structure and identify the motif to target, then I …