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Quantification And Identification Of Microproteinuria Using Ultrafiltration And Atr-Ftir Spectroscopy, David Perezguaita, Zack Richardson, Phil Heraud, Bayden Wood

Articles

The presence of low amounts of specific proteins in urine can be an indicator of diagnosis and prognosis of several diseases including renal failure and cancer. Hence, there is an urgent need for Point-of-care (PoC) methods, which can quantify microproteinuria levels (30-300 ppm) and identify the major proteins associated with the microproteinuria. In this study, we coupled ultracentrifugation with attenuated total reflectance-Fourier transform infrared (ATR-FTIR) to identify and quantify proteins in urine at low parts per million levels. The process involves the preconcentration of proteins from 500 μL of urine using an ultrafiltration device. After several washings, the isolated proteins …

Validity And Reliability Study Of The Turkish Version Of The Self-Efficacy For Managing Chronic Disease 6-Item Scale, Kübra İnci̇rkuş, Nursen Nahci̇van

Turkish Journal of Medical Sciences

Background/aim: The measurement of self-efficacy is an important step toward improving chronic disease management, enhancing adherence to treatment, and assessing patients with chronic diseases. The purpose of this study was to assess the validity and reliability of the Turkish version of theSelf-Efficacy for Managing Chronic Disease 6-Item Scale. Materials and methods: In this methodological study, the sample consisted of 211 patients receiving chronic disease care in 2 family health centers in İstanbul, Turkey. Data were analyzed with descriptive statistics, exploratory and confirmatory factor analyses, concurrent validity, Cronbach's alpha, item-total correlation, and test-retest reliability.Results: Most of the sample were women (72%), …

Determining The Age Of Spoiled Milk From Dried Films Using Attenuated Reflection Fourier Transform Infrared (Atr Ft-Ir) Spectroscopy, Zack Richardson, David Perezguaita, Kamila Kochan, Bayden Wood

Articles

Milk spoilage is an inevitable occurrence, which generates waste and can result in food poisoning. When milk spoils, the off-flavor and curdling are due to excessive proliferation of various bacteria which causes pH changes. Time, temperature, environment,and previous handling practice all affects the spoilage rate. There is a need for a fast reliable and accurate method that can identify in situearly spoilage of milk. Here we show the ability of attenuated total reflectionFourier transformed infrared spectroscopy(ATR FT-IR) in conjunction with multivariate data analysis to predict the age of milk. We found that dried films vastly increased the absorbance of important …

Herpes Simplex Virus 1 Targets Irf7 Via Icp0 To Limit Type I Ifn Induction, David Shahnazaryan, Rana Khalil, Claire Wynne, Caroline A Jefferies, Joan Ní Gabhann-Dromgoole, Conor C Murphy

Articles

Herpes simplex keratitis (HSK), caused by herpes simplex virus type 1 (HSV‑1) infection, is the commonest cause of infectious blindness in the developed world. Following infection the virus is initially suspended in the tear film, where it encounters a multi‑pronged immune response comprising enzymes, complement, immunoglobulins and crucially, a range of anti‑viral and pro‑inflammatory cytokines. However, given that HSV‑1 can overcome innate immune responses to establish lifelong latency throughout a susceptible individual’s lifetime, there is significant interest in understanding the mechanisms employed by HSV‑1 to downregulate the anti‑viral type I interferon (IFN) mediated immune responses. This study aimed to investigate …

Travail Saisonnier Et Santé Au Travail : Bilan Des Connaissances Et Développement D’Une Méthode D’Analyse Pour Le Suivi Longitudinal Des Troubles Musculosquelettiques : Supplément De Rapport, Marie-Eve Major, Pascal Wild, Hélène Clabault

Rapports de recherche scientifique

Le travail saisonnier, bien qu’il puisse à première vue sembler relativement marginal sur le marché du travail, est central pour plusieurs régions et secteurs d’activité. Communément associé aux activités d’exploitation des ressources naturelles (agriculture, foresterie, pêche), le travail saisonnier est également présent dans plusieurs autres secteurs d’activité (fabrication, transformation alimentaire, services publics, etc.). Ce type de travail se différencie de celui qui est permanent par sa temporalité, mais aussi par son incertitude liée à la saisonnalité. Les travailleurs saisonniers sont reconnus pour leurs longues heures de travail et l’alternance entre les périodes « saison » et « hors saison ». …

Travail Saisonnier Et Santé Au Travail : Bilan Des Connaissances Et Développement D’Une Méthode D’Analyse Pour Le Suivi Longitudinal Des Troubles Musculosquelettiques, Marie-Eve Major, Pascal Wild, Hélène Clabault

Rapports de recherche scientifique

Le travail saisonnier, bien qu’il puisse à première vue sembler relativement marginal sur le marché du travail, est central pour plusieurs régions et secteurs d’activité. Communément associé aux activités d’exploitation des ressources naturelles (agriculture, foresterie, pêche), le travail saisonnier est également présent dans plusieurs autres secteurs d’activité (fabrication, transformation alimentaire, services publics, etc.). Ce type de travail se différencie de celui qui est permanent par sa temporalité, mais aussi par son incertitude liée à la saisonnalité. Les travailleurs saisonniers sont reconnus pour leurs longues heures de travail et l’alternance entre les périodes « saison » et « hors saison ». …

Speaking Points, Dr. Samar Fatima, Farzana Irfan

COVID-19 Archive Project

First hospital to treat a COVID-19 patient in Pakistan.

First hospital to partner with the government for its public vaccination programme.

We were the first ones to identify the Omicron variant in Pakistan.

We were among the first voices to talk about COVID.

An Insight Into The Epitope-Based Peptide Vaccine Design Strategy And Studies Against Covid-19, Murat Topuzoğullari, Tayfun Acar, Peli̇n Peli̇t Arayici, Burcu Uçar, Erennur Uğurel, Emrah Şefi̇k Abamor, Tüli̇n Arasoğlu, Di̇lek Balik, Serap Derman

Turkish Journal of Biology

SARS-CoV-2 is a new member of the coronavirus family and caused the pandemic of coronavirus disease 2019 (COVID-19) in 2020. It is crucial to design and produce an effective vaccine for the prevention of rapid transmission and possible deaths wcaused by the disease. Although intensive work and research are being carried out all over the world to develop a vaccine, an effective and approved formulation that can prevent the infection and limit the outbreak has not been announced yet. Among all types of vaccines, epitope-based peptide vaccines outshine with their low-cost production, easy modification in the structure, and safety. In …

South Atlantic Mpas And Oculina Hapc: Characterization Of Fish Communities, Benthic Habitat, And Benthic Macrobiota Final Report For 2018-2019 Noaa Ship Pisces Cruises (18-02, 19-02)., Stacey Harter, John K. Reed, Stephanie Farrington

Faculty Scholarship

The South Atlantic Fishery Management Council (SAFMC) and Department of Commerce through the Magnuson-Stevens Fishery Management Act have established eight deepwater Marine Protected Areas (MPAs), five deepwater Coral Habitat Areas of Particular Concern (CHAPCs), and the Oculina Coral HAPC along the outer continental shelf off the southeastern U.S. This project proposed to document and characterize the benthic habitat, benthic sessile biota, and fish populations within some of these protected areas and within the jurisdiction of the SAFMC. In February 2009, the SAFMC implemented eight Type II MPAs between Cape Hatteras, NC and the Florida Keys to protect seven species of …

A Parsimonious Equity?: Discussion Of Equity: Conscience Goes To Market, Samuel L. Bray

Journal Articles

Equity has always had enemies. As Professor Irit Samet notes in her agile and insightful new book, Equity: Conscience Goes to Market, these enemies have lodged two main complaints. One is that equity is not distinctive, and for that reason should be eliminated. The other is that equity is distinctive—and dangerous—and for that reason should be eliminated.

Today And Yesterday, Vol. 44, 2020, Illinois State University, Department Of History

Today and Yesterday

Newsletter of the Illinois State University Department of History

The Complexities Of Democracy, Development, And Human Rights In China's Belt And Road Initiative, Diane Desierto

Journal Articles

China's Belt and Road Initiative (BRI)-a dense network of China-funded multi-year infrastructure projects in over 65 countries from the Western Pacific to the Baltic Sea, collectively aiming to establish China's strategic "Maritime Belt" and "Silk Road" connectivity using an estimated range of USD$1 Trillion to USD$8 Trillion--is as unprecedented phenomenon in sovereign project financing and bilateral investment lending, since the United States' grant of USD $800 Billion for the Marshall Plan was for Europe's post World War II reconstruction. The scale, scope, and terms of BRI projects remain shrouded in relative opacity, with China as of this writing only incrementally …

China's Maritime Law Enforcement Activities In The South China Sea, Diane Desierto

Journal Articles

China, one of the territorial and maritime claimants of the South China Sea, declared in April 2020 that it had created two new administrative districts in the South China Sea:

Xisha district, covering the Paracel Islands and Macclesfield Bank, and Nansha district covering the Spartly [sic] Islands .... The new administrative districts are to be under the authority of the local government in Sansha, a city located on Woody Island which is administratively part of Hainan province. The Xisha district will be based in Sansha while the Nansha district will operate from Fiery Cross Reef in the Spratlys.

Sansha City …

Religious Freedom And The Churches: Contemporary Challenges In The United States Today, Richard W. Garnett

Journal Articles

A crucial, but often overlooked, dimension of the human and constitutional right to religious freedom is the autonomy of religious institutions, associations and societies with respect to matters of governance, doctrine, formation and membership. Although the Supreme Court of the United States has affirmed this autonomy in the context of American constitutional law, it is vulnerable, and even under threat, for a variety of reasons, including a general decline in the health of civil society and mediating associations and a crisis of confidence and authority caused by clerical sexual abuse and churches’ failure to respond to it.

The Communitarian Work And Vision(S) Of Robert Cochran (And Thomas Shaffer), Richard W. Garnett

Journal Articles

Professor Robert Cochran's work and thought were powerfully shaped by those of his friend, mentor, and teacher, the late Professor Thomas Shaffer, a towering figure in the religious lawyering movement. A leading theme in Shaffer's writing, one that has continued through and been developed in Cochran's, is "community." This Essay explores and unpacks this theme and highlights several ways that the idea of "community" functions in their vision of the lawyer 's role and vocation.

Neoclassical Administrative Law, Jeffrey A. Pojanowski

Journal Articles

This Article introduces an approach to administrative law that reconciles a more formalist, classical understanding of law and its supremacy with the contemporary administrative state. Courts adopting this approach, which I call “neoclassical administrative law,” are skeptical of judicial deference on questions of law, tend to give more leeway to agencies on questions of policy, and attend more closely to statutes governing administrative procedure than contemporary doctrine does. As a result, neoclassical administrative law finds a place for both legislative supremacy and the rule of law within the administrative state, without subordinating either of those central values to the other. …

An Economic Approach To Religious Exemptions, Stephanie H. Barclay

Journal Articles

Externalities caused by religious exemptions have been getting the spotlight again in light a case the U.S. Supreme Court will hear this term: Fulton v. City of Philadelphia. Some argue that religious individuals should be required to internalize the costs they impose on third parties and thus should be denied the right to practice that harmful behavior. These new progressive theories about harm trade on rhetoric and normative intuitions regarding externalities and costs. But curiously, these theories also largely ignore an influential theoretical movement that has studied externalities and costs for the last fifty years: law and economics.

This Article …

Untangling Entanglement, Stephanie H. Barclay

Journal Articles

The Court has increasingly signaled its interest in taking a more historical approach to the Establishment Clause. And in its recent American Legion decision, the Supreme Court strongly suggested that the three-prong Lemon test is essentially dead letter. Such a result would make sense for the first two prongs of the Lemon test about secular purpose and the effects. Many scholars have observed that these aspects of the prong are judicial creations far afield of the Establishment Clause history. But what of the entanglement prong of the test? If we rejected all applications of this prong of the analysis, would …

The Comparative Legal Landscape Of Educational Pluralism, Nicole Stelle Garnett

Journal Articles

In the United States, debates about private and faith-based education tend to focus on questions about government funding: which kinds of schools should the government fund (and at what levels)? Should, for example, students be able to use public funds to attend privately operated schools? Faith-based schools? If so, what policy mechanisms should be used to fund private schools—vouchers, tax credits, direct transfer payments? How much funding should these schools receive? The same amount as public schools or less? As a historical matter, the focus on funding in the United States makes sense because only public (that is, government-operated) elementary …

Correlation Between Number Of Mendeley Readers And Citations In Dental Sciences, Jafar Kolahi, David G. Dunning, Edward F. Rossomando

Journal Articles: College of Dentistry

No abstract provided.

Heightened Levels Of Antimicrobial Response Factors In Patients With Rheumatoid Arthritis, Prathapan Ayyappan, Robert Z. Harms, Jennifer A. Seifert, Elizabeth A. Bemis, Marie L. Feser, Kevin D. Deane, M Kristen Demoruelle, Ted R. Mikuls, V Michael Holers, Nora Sarvetnick

Journal Articles: Surgery

Rheumatoid arthritis (RA) is a chronic progressive autoimmune disease leading to considerable disability over time. The disease can be characterized by the presence of multiple autoantibodies in the serum and synovial fluid. Microbial dysbiosis is proposed to play a role in the pathogenesis of RA. Increased systemic bacterial exposure leads to elevated levels of antimicrobial response factors (ARFs) in the circulation. In the present study, we tested whether RA patients have increased levels of ARFs by analyzing the levels of multiple ARFs in serum from RA patients and healthy age and sex-matched controls. The levels of soluble CD14 (sCD14), lysozyme, …

Complexation-Induced Resolution Enhancement Of 3d-Printed Hydrogel Constructs, Jiaxing Gong, Carl C. L. Schuurmans, Anne Metje Van Genderen, Xia Cao, Wanlu Li, Feng Cheng, Jacqueline Jialu He, Arturo López, Valentin Huerta, Jennifer Manríquez, Ruiquan Li, Hongbin Li, Clément Delavaux, Shikha Sebastian, Pamela E. Capendale, Huiming Wang, Jingwei Xie, Mengfei Yu, Rosalinde Masereeuw, Tina Vermonden, Yu Shrike Zhang

Journal Articles: Surgery

Three-dimensional (3D) hydrogel printing enables production of volumetric architectures containing desired structures using programmed automation processes. Our study reports a unique method of resolution enhancement purely relying on post-printing treatment of hydrogel constructs. By immersing a 3D-printed patterned hydrogel consisting of a hydrophilic polyionic polymer network in a solution of polyions of the opposite net charge, shrinking can rapidly occur resulting in various degrees of reduced dimensions comparing to the original pattern. This phenomenon, caused by complex coacervation and water expulsion, enables us to reduce linear dimensions of printed constructs while maintaining cytocompatible conditions in a cell type-dependent manner. We …

Advanced Preparation Makes Research In Emergencies And Isolation Care Possible: The Case Of Novel Coronavirus Disease (Covid-19), David Brett-Major, Elizabeth R. Schnaubelt, Hannah M. Creager, Abigail Lowe, Theodore J. Cieslak, Jacob M. Dahlke, Daniel W. Johnson, Paul D. Fey, Keith F. Hansen, Angela L. Hewlett, Bruce G. Gordon, Andre C. Kalil, Ali S. Khan, Mark G. Kortepeter, Christopher J. Kratochvil, Luann Larson, Deborah A. Levy, James Linder, Sharon J. Medcalf, Mark E. Rupp, Michelle M. Schwedhelm, James N. Sullivan Md, Angela M. Vasa, Michael C. Wadman, Rachel E. Lookadoo, John-Martin J. Lowe, James V. Lawler, M. Jana Broadhurst

Journal Articles: Epidemiology

The optimal time to initiate research on emergencies is before they occur. However, timely initiation of high-quality research may launch during an emergency under the right conditions. These include an appropriate context, clarity in scientific aims, preexisting resources, strong operational and research structures that are facile, and good governance. Here, Nebraskan rapid research efforts early during the 2020 coronavirus disease pandemic, while participating in the first use of U.S. federal quarantine in 50 years, are described from these aspects, as the global experience with this severe emerging infection grew apace. The experience has lessons in purpose, structure, function, and performance …

Molecular Implications Of Muc5ac-Cd44 Axis In Colorectal Cancer Progression And Chemoresistance, Ramesh Pothuraju, Satyanarayana Rachagani, Shiv Ram Krishn, Sanjib Chaudhary, Rama Krishna Nimmakayala, Jawed A. Siddiqui, Koelina Ganguly, Imayavaramban Lakshmanan, Jesse L. Cox, Kavita Mallya, Sukhwinder Kaur, Surinder K. Batra

Journal Articles: Biochemistry & Molecular Biology

BACKGROUND: Differential expression of mucins has been associated with several cancers including colorectal cancer (CRC). In normal physiological conditions, secretory mucin MUC5AC is not expressed in the colonic mucosa, whereas its aberrant expression is observed during development of colon cancer and its precursor lesions. To date, the molecular mechanism of MUC5AC in CRC progression and drug resistance remains obscure.

METHODS: MUC5AC expression was determined in colon tissue microarray by immunohistochemistry. A RNA interference and CRISPR/Cas9-mediated system was used to knockdown/knockout the MUC5AC in CRC cell lines to delineate its role in CRC tumorigenesis using in vitro functional assays and in …

Nr2e3 Is A Genetic Modifier That Rescues Retinal Degeneration And Promotes Homeostasis In Multiple Models Of Retinitis Pigmentosa, Sujun Li, Shyamtanu Datta, Emily Brabbit, Zoe Love, Victoria Woytowicz, Kyle Flattery, Jessica Capri, Katie Yao, Siqi Wu, Michael Imboden, Arun Upadhyay, Rasappa Arumugham, Wallace B. Thoreson, Margaret M Deangelis, Neena B. Haider

Journal Articles: Ophthalmology

Recent advances in viral vector engineering, as well as an increased understanding of the cellular and molecular mechanism of retinal diseases, have led to the development of novel gene therapy approaches. Furthermore, ease of accessibility and ocular immune privilege makes the retina an ideal target for gene therapies. In this study, the nuclear hormone receptor gene Nr2e3 was evaluated for efficacy as broad-spectrum therapy to attenuate early to intermediate stages of retinal degeneration in five unique mouse models of retinitis pigmentosa (RP). RP is a group of heterogenic inherited retinal diseases associated with over 150 gene mutations, affecting over 1.5 …

Title Ix And Official Policy Liability: Maximizing The Law’S Potential To Hold Education Institutions Accountable For Their Responses To Sexual Misconduct, Erin E. Buzuvis

Faculty Scholarship

Title IX, the federal statute that prohibits sex discrimination in education, plays a key role in institutional accountability for sexual misconduct that is perpetrated by a school’s students, faculty, and staff. The Supreme Court has confirmed that Title IX includes an implied right of action for money damages when the institution had actual notice that sexual harassment had occurred, or was likely to occur, and responded to that threat with deliberate indifference. But the deliberate indifference standard has proven to be a high and unpredictable bar for plaintiffs. For this reason, many institutions required the threat of government enforcement—issued in …

Foreword, Sudha Setty

Faculty Scholarship

In November 2019, the Western New England Law Review held its symposium, On Account of Sex: Women’s Suffrage and the Role of Gender in Politics Today. The symposium articles ask us to look at history to see what factors enabled path-breaking activists to secure the right to vote in a time of immense national turmoil. They also ask us to weigh how history should assess the strategic decisions that ultimately gained political rights for some women, but deliberately excluded Black women and other activists.

These historical accounts help us consider how the right to vote is faring, particularly after …

Doesn't Everybody Want To Be Healthy?, Jana L. Wardian Phd

Journal Articles: Hospital Medicine

No abstract provided.

Preconception Counseling For Women With Diabetes, Abegail L. Hubberd, Nina A. Watson, Ellen Cobb, Jana L. Wardian Phd, Connie C. Morrow, Tom J. Sauerwein

Journal Articles: Hospital Medicine

"Quality Improvement Success Stories" are published by the American Diabetes Association in collaboration with the American College of Physicians, Inc., and the National Diabetes Education Program. This series is intended to highlight best practices and strategies from programs and clinics that have successfully improved the quality of care for people with diabetes or related conditions. Each article in the series is reviewed and follows a standard format developed by the editors of

The Choice Should Be Yours: Diabetes-Related Distress By Insulin Delivery Method For People With Type 1 Diabetes, Jana L. Wardian Phd, Mark W. True, Irene Folaron, Jeff Colburn, Joshua M. Tate, Darrick J. Beckman

Journal Articles: Hospital Medicine

Background: American Diabetes Association (ADA) recommends psychosocial assessment for people with diabetes, including diabetes-related distress. Elevated diabetes-related distress is associated with poor self-management, lower medication adherence, and poorer quality of life. Insulin delivery methods are multiple daily injections (MDI) or continuous subcutaneous insulin infusion (CSII). Because people with type 1 diabetes mellitus (T1DM) require comprehensive insulin therapy to manage blood glucose, we explored the association of insulin delivery methods and diabetes distress in this group. Methods: The U.S. Air Force Diabetes Center of Excellence (DCOE), a specialty clinic for adults who are Military Health System beneficiaries, administers the validated 17-item …