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Visual Arts Enhance Instruction In Observation And Analysis Of Microscopic Forms In Developmental And Cell Biology, Max Ezin, Christina Noravian, Amira Mahomed, Adam Lyle, Aveleen Gill, Tamira Elul

The Transdisciplinary STEAM+ Journal

Two important skills for scientists in developmental and cell biology, as well as in fields such as neurobiology, histology and pathology, are: 1) observation of features and details in microscopic images of cells, and 2) quantification of cellular features observed in microscopic images. However, current training in developmental and cell biology does not emphasize observation and quantitative analysis of microscopic images, and it is unclear how best to teach students these skills. Here, we describe our experiences applying visual artistic approaches to instruct undergraduate and graduate students in how to observe and analyze cellular forms in microscopic images. At Loyola …

Obtaining And Propertics Polymermetalcomplex On The Base Carboxymethylcellulose And Selenium, Abdishukur Abduhalilovich Sarymsakov, Khaydar Ergashovich Yunusov, Foziljon Mamarayim O’G'Li Turakulov, Javlon Zafar O’G’Li Jalilov, Ziyovuddin Zayniddinovich Hakimov, Bychkovsky Mihayilovich Pavel, Mamarayim Aynaqulovich Turakulov

Bulletin of Gulistan State University

In this study, technical sodium carboxymethylcellulose (Na-CMC) with degree of substitution (DS) = 0.62-0.90 and a degree of polymerization (DP) = 200-600 were washed in 70% ethyl alcohol and a 2% fraction of purified Na-CMC was centrifugation at a speed of 6000 rpm for 20 minutes. A 0.1 M solution of SeO2 was added to the centrifuged Na-CMC solution at 60 ⁰C and mixed mechanically. Samples of gels, films and powders containing a solution of Na-CMC containing stabilized selenium nanoparticles synthesized by chemical reduction were taken and their physicochemical properties were studied. The size and shape of selenium nanoparticles present …

Obtaining And Propertics Polymermetalcomplex On The Base Carboxymethylcellulose And Selenium, Abdishukur Abduhalilovich Sarymsakov, Khaydar Ergashovich Yunusov, Foziljon Mamarayim O’G'Li Turakulov, Javlon Zafar O’G’Li Jalilov, Ziyovuddin Zayniddinovich Hakimov

Bulletin of Gulistan State University

In this study, technical sodium carboxymethylcellulose (Na-CMC) with degree of substitution (DS) = 0.62-0.90 and a degree of polymerization (DP) = 200-600 were washed in 70% ethyl alcohol and a 2% fraction of purified Na-CMC was centrifugation at a speed of 6000 rpm for 20 minutes. A 0.1 M solution of SeO2 was added to the centrifuged Na-CMC solution at 60 ⁰C and mixed mechanically. Samples of gels, films and powders containing a solution of Na-CMC containing stabilized selenium nanoparticles synthesized by chemical reduction were taken and their physicochemical properties were studied. The size and shape of selenium nanoparticles present …

Mouse Mutant Phenotyping At Scale Reveals Novel Genes Controlling Bone Mineral Density., Anna L Swan, Christine Schütt, Jan Rozman, Maria Del Mar Muñiz Moreno, Stefan Brandmaier, Michelle Simon, Stefanie Leuchtenberger, Mark Griffiths, Robert Brommage, Piia Keskivali-Bond, Harald Grallert, Thomas Werner, Raffaele Teperino, Lore Becker, Gregor Miller, Ala Moshiri, John R Seavitt, Derek D Cissell, Terrence F Meehan, Elif F Acar, Christopher J Lelliott, Ann M Flenniken, Marie-France Champy, Tania Sorg, Abdel Ayadi, Robert E Braun, Heather Cater, Mary E Dickinson, Paul Flicek, Juan Gallegos, Elena J Ghirardello, Jason D Heaney, Sylvie Jacquot, Connor Lally, John G Logan, Lydia Teboul, Jeremy Mason, Nadine Spielmann, Colin Mckerlie, Stephen A Murray, Lauryl M J Nutter, Kristian F Odfalk, Helen Parkinson, Jan Prochazka, Corey L Reynolds, Mohammed Selloum, Frantisek Spoutil, Karen L. Svenson, Taylor S Vales, Sara E Wells, Jacqueline K White, Radislav Sedlacek, Wolfgang Wurst, Kent K C Lloyd, Peter I Croucher, Helmut Fuchs, Graham R Williams, Duncan Bassett, Valerie Gailus-Durner, Yann Herault, Ann-Marie Mallon, Steve D M Brown, Philipp Mayer-Kuckuk, Martin Hrabe De Angelis, Impc Consortium

Faculty Research 2020

The genetic landscape of diseases associated with changes in bone mineral density (BMD), such as osteoporosis, is only partially understood. Here, we explored data from 3,823 mutant mouse strains for BMD, a measure that is frequently altered in a range of bone pathologies, including osteoporosis. A total of 200 genes were found to significantly affect BMD. This pool of BMD genes comprised 141 genes with previously unknown functions in bone biology and was complementary to pools derived from recent human studies. Nineteen of the 141 genes also caused skeletal abnormalities. Examination of the BMD genes in osteoclasts and osteoblasts underscored …

Exploration Of Local Microorganisms From Rumen And Their Potential To Make Silage From Agricultural Waste, Purnamasari Listya, Purnomo Hari, Setyawan Hidayat Bambang

ASEAN Journal on Science and Technology for Development

Agricultural wastes are very diverse in type and have low nutrient values, especially in lignin and cellulose, which are difficult to digest. One way of overcoming this problem is by biological treatment using microorganisms, also known as silage. The availability of bio-activators that are both cost-efficient and easy to make further suggests that using local microorganisms may be an effective possible solution. This study focused on processing food crop waste (corn leaves) into silage using local microorganisms as the bio-activators. Research took place in the Agrotechnology Laboratory, University of Jember. The experimental design was completely randomized with four treatments: Treatment …

Antimalarials And Phytotoxins From Botryosphaeria Dothidea Identified From A Seed Of Diseased Torreya Taxifolia, Mallika Kumarihamy, Luiz H. Rosa, Natascha Techen, Daneel Ferreira, Edward M. Croom, Stephen O. Duke, Babu L. Tekwani, Shabana Khan, N. P.Dhammika Nanayakkara

Faculty and Student Publications

The metabolic pathways in the apicoplast organelle of Plasmodium parasites are similar to those in plastids in plant cells and are suitable targets for malaria drug discovery. Some phytotoxins released by plant pathogenic fungi have been known to target metabolic pathways of the plastid; thus, they may also serve as potential antimalarial drug leads. An EtOAc extract of the broth of the endophyte Botryosphaeria dothidea isolated from a seed collected from a Torreya taxifolia plant with disease symptoms, showed in vitro antimalarial and phytotoxic activities. Bioactivity-guided fractionation of the extract afforded a mixture of two known isomeric phytotoxins, FRT-A and …

Imaging The Tumor Microenvironment Using 1,2-Dioxetane Chemiluminescence Agents, Lucas Ryan

Chemistry Theses and Dissertations

As complex organisms, animals and humans rely on a delicate interplay between many connected systems to maintain proper cellular and whole-body function. Oxygen regulation and homeostasis are vital for proper function in healthy cells, as are core components of aerobic metabolism and efficient ATP generation to supply our energy needs. Maintaining pH homeostasis is also of primary importance; cells maintain pH homeostasis through a host of active and passive transporters to maintain pH balance both inside and outside of the cell.

Events of uncontrolled cellular proliferation, also known as cancer, survive and thrive under conditions that perturb the delicate balance …

Oral Chemotherapy: Safe Handling In The Home, Cynthia Huff

DNP Qualifying Manuscripts

Background: According to the National Institute for Occupational Safety and Health (2016), oral anticancer agents (OAC) are hazardous drugs (HD) and require the same safe handling interventions in the home setting as in controlled health care environments. The use of a safe handling checklist for OAC administration is a way nurses can provide safe handling education to patients and caregivers and assess for understanding and competency for critical tasks. Effective home management strategies outlining caregiver protections, proper storage, administration of OACs, spill clean-up, and waste disposal could reduce exposure risks to toxic agents.

Objective: To present a safe-handling checklist …

Usp <800> Compliance: A Hazardous Drug Safe Handling Ppe Toolkit For Infusion Nurses, Cynthia Huff

Doctor of Nursing Practice (DNP) Projects

Problem: Safety concerns have existed for more than 40 years about how hazardous drug (HD) exposure contributes to long- and short-term adverse health outcomes for healthcare workers (HCWs). Careless handling may cause toxic residues to infiltrate hospital environments and patient care areas, and can even be traced to patients’ homes. New government regulations will require healthcare organizations to minimize exposure risks to HCWs by fully implementing the U.S. Pharmacopeia (USP) Convention Chapter 800: Hazardous Drugs: Handling in Healthcare Settings (USP, 2016) on December 1, 2019. According to Polovich and Olsen (2017), “The implementation of the USP <800> Standards will represent …

Newly Generated Atractylon Derivatives In Processed Rhizomes Of Atractylodes Macrocephala Koidz, Chunmei Zhai, Jianping Zhao, Amar G. Chittiboyina, Yonghai Meng, Mei Wang, Ikhlas A. Khan

Faculty and Student Publications

Thermally processed rhizomes of Atractylodes macrocephala (RAM) have a long history of use in traditional Chinese medicine (TCM) for treating various disorders, and have been an integral part of various traditional drugs and healthcare products. In TCM, herbal medicines are, in most cases, uniquely processed. Although it is thought that processing can alter the properties of herbal medicines so as to achieve desired functions, increase potency, and/or reduce side effects, the underlying chemical changes remain unclear for most thermally processed Chinese herbal medicines. In an attempt to shed some light on the scientific rationale behind the processes involved in traditional …

Intergenerational Trauma, Ruqia Abdirahman

Counselor Education Capstones

The following literature review will explore cumulative research on intergenerational trauma. Although, many communities have encountered massive traumas the research has mainly focused on Canadians First Nations people and Holocaust survivors. The effects of trauma can be multifaceted, impacting an individual’s psychological and emotional well-being across generations. Furthermore, this paper will discuss the impact of intergenerational trauma among various communities, ways trauma transmission occurs, and review of the Intergenerational Trauma Treatment Model.

Usp<800> Compliance: A Hazardous Drug Safe-Handling Quality Improvement Project For Ambulatory Care Infusion Nurses, Cynthia Huff

Doctor of Nursing Practice (DNP) Projects

Problem: The U.S. Pharmacopeia (USP, 2016) Convention Chapter 800: Hazardous Drugs: Handling in Healthcare Settings took effect on December 1, 2019. According to Polovich and Olsen (2017), " The implementation of the USP <800> Standards. will represent an important step forward to protect nurses and other potentially exposed healthcare workers (HCWs): (p.1).

Context: The Doctor of Nursing Practice (DNP) project implemented a hazardous drug (HD) safe-handling personal protective equipment (PPE) toolkit in accordance to USP <800>standards and hospital policies at an ambulatory cancer infusion center to improve nurses' adherence with PPE use.

Interventions: The project consisted of (a) a safe-handling PPE …

What Is The Efficacy Of Biologics For Chronic Plaque-Type Psoriasis Among Pediatric Patients?, Uduakobong Akpainyang

Health Informatics Student Papers and Projects

Chronic plaque psoriasis is an inflammatory skin disease characterized by red thick, raised, silvery scaly patches. It is the most common type of psoriasis, often appearing on the knees, postauricular area, elbows, scalp, and lower back. Chronic plaque psoriasis was the most prevalent (73.7%) among children with psoriasis and was found to increase with age in a study at Olmsted County, Minnesota (Tollefson et al., 2010). Chronic plaque psoriasis is a life-changing condition that significantly impacts the overall health and well-being (emotional, physical, social, and psychological) as well as quality of life in pediatric patients. As a result of data …

3d Cell Culture: Techniques, Applications In Healthcare, And Future Challenges, Mukunthan K S

Manipal Journal of Science and Technology

Two-dimensional (2D) cell cultures have been the most commonly used technique since cell culture was first introduced. However, these cultures do not accurately represent animal physiology as many factors that play an important role in the growth of animal cells are overlooked. Three-dimensional (3D) cell culture techniques provide higher accuracy in recreating the microenvironment of native tissues. But, 2D cell culture techniques are still preferred for the majority of animal cell cultures as these are well established and relatively inexpensive. Various techniques have been developed for 3D cell culture but these systems are highly complex as the number of parameters …

Electron-Rich Alkynes And Azodicarboxylates Chemistry Towards Vicinal Diamine Motif Containing Small Molecules And N-Heterocycles, Chandima Jeewantha Narangoda

All Dissertations

Heterocyclic molecules that contain nitrogens and consist of four, five and six-membered rings such as azetidinones (eg: β-lactams), pyrroles, indoles, azoles, triazoles, oxadiazines, and oxadiazinones, etc. are ubiquitous in the use of pharmaceutical fields. This also reflected in the recently FDA approved unique small-molecule pharmaceuticals, where about 90% of those molecules contain a nitrogen heterocycle. Particularly, the vicinal diamino motif has been appeared in leading drug directing candidates such as penicillin, cephalosporins, and oseltamivir (Tamiflu) over the past years.

Historically, there has been minimal interest in synthesizing a broad scope of vicinal diamino motif-containing small molecules and heterocyclic compounds for …

The Promise Of A Multi-Disciplinary, Mixed-Methods Approach To Inform Insect Pest Management: Evidence From Wyoming Alfalfa, Randa Jabbour, Shiri Noy

Faculty Publications

No abstract provided.

The Application And Development Of Metabolomics Methodologies For The Profiling Of Food And Cellular Toxicity, Jade Woods

Department of Chemistry: Dissertations, Theses, and Student Research

Metabolomics is a rapidly growing field of study. Its growth reflects advancements in technology and an improved understanding of the impact of the environment on metabolism. As a result, metabolomics is now commonly employed to investigate and characterize human and plant metabolism. The first chapter of this thesis provides an introduction to metabolomics and an overview of the protocols for sample preparation, data collection and statistical analysis. The second thesis chapter describes in explicit detail the step-by-step process of extracting and analyzing metabolites collected from mammalian cells, specifically brain tissue with a focus on Parkinson’s disease. The chapter highlights important …

Design, Synthesis And Antibacterial Studies Of Some New Pyridopyrimidine Derivatives As Biotin Carboxylase Inhibitors, Vivek B. Panchabhai, Parag G. Ingole, S R. Butle

Bulletin of Faculty of Pharmacy Cairo University

Present study reports the development of novel pyridopyrimidine derivatives as biotin carboxylase inhibitors with potent antibacterial activity. These compounds were designed to avoid possibility of resistance development. Accordingly eighteen compounds were synthesised and characterized on the basis of spectral data. These compounds were tested for their antibacterial potential by the enzyme kinetic assay against the biotin carboxylase. The minimum inhibitory concentration (MIC) and single step resistance studies were also performed. Compound 2-((2-Phenylpyrido[2,3-d]pyrimidin-4-yl)amino)phenol (6o) showed promising activity in biotin carboxylase inhibition with low MIC. It showed molecular docking score of -7.96, this compound showed formation of hydrogen bonds with the active …

Development Of Computational To Ols To Target Microrna, Luo Song

Dissertations and Theses (Open Access)

MicroRNAs (a.k.a, miRNAs) play an important role in disease development. However, few of their structures have been determined and structure-based computational methods remain challenging in accurately predicting their interactions with small molecules. To address this issue, my thesis is to develop integrated approaches to screening for novel inhibitors by targeting specific structure motifs in miRNAs. The project starts with implementing a tool to find potential miRNA targets with desired motifs. I combined both sequence information of miRNAs and known RNA structure data from Protein Data Bank (PDB) to predict the miRNA structure and identify the motif to target, then I …

Triggerable, Targetable, And Trackable Flavonol-Based Carbon Monoxide-Releasing Molecules, Livia S. Lazarus

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Long known as a toxic gas, carbon monoxide (CO) has been recently redefined as a health beneficial molecule produced endogenously in humans. The biological activity of CO has been extensively examined in inflammatory conditions, vasoprotection, anti-apoptotic and anticancer studies, although the mechanisms of action are still not well defined. Because CO is a gaseous molecule, a straightforward administration would be inhalation. However, this delivery method is limited to use in hospital settings under professional supervision. For improved control over the amount and location of CO delivery, metal-free and light-triggered carbon monoxide-releasing molecules are of particular current interest. Our lab is …

Determining A Method For Expressing And Purifying Cytochrome P450 4v2: A Protein Involved In Bietti's Crystalline Dystrophy, Cody Lane Turner

Graduate Theses/Dissertations

Within the Cytochrome P450 class of enzymes, there are a group known as the “orphan” cytochromes. The “orphan” classification comes from the poorly understood in vivo functionality and substrate specificity. Cytochrome P450 4V2 (CYP4V2) is one of these “orphans” and belongs to the CYP4 family. The CYP4 family is known for the omega oxidation of endogenous fatty acids. This family is most commonly found on chromosome 1 (CYP4ABXZ). CYP4V2 is unique in that its location is bound to chromosome 4 as discovered by Jiao in 2004. Mutations within the CYP4V2 gene have been associated with the …

The Use Of It Tools In The Assessment And Development Of Leadership Abilities, Stanisław Stanek, Agnieszka Sabat

European Management Studies

This paper aims to present the findings of research into the possibilities of deploying IT tools to support the assessment and development of leadership abilities. The research procedure consisted in an analysis of four case studies from the business and military sectors. The primary research methods were a case study analysis, participant observation, Development Centre, and a source literature analysis. Under individual case studies, the following techniques were applied: individual and group interviews, brainstorming, and role playing. The tools applied included psychometric software (Thomas International, Extended Disc, Thalento) and military simulators. The division of roles between man and computer was …

Plant Single-Cell Metabolomics—Challenges And Perspectives, Leonardo Perez De Souza, Monica Borghi, Alisdair R. Fernie

Biology Faculty Publications

Omics approaches for investigating biological systems were introduced in the mid-1990s and quickly consolidated to become a fundamental pillar of modern biology. The idea of measuring the whole complement of genes, transcripts, proteins, and metabolites has since become widespread and routinely adopted in the pursuit of an infinity of scientific questions. Incremental improvements over technical aspects such as sampling, sensitivity, cost, and throughput pushed even further the boundaries of what these techniques can achieve. In this context, single-cell genomics and transcriptomics quickly became a well-established tool to answer fundamental questions challenging to assess at a whole tissue level. Following a …

Posttranslational Modification And Protein Disorder Regulate Protein-Protein Interactions And Dna Binding Specificity Of P53, Robin Levy

USF Tampa Graduate Theses and Dissertations

p53 is an intrinsically disordered transcription factor that suppresses tumor development by arresting the cell cycle and promoting DNA repair. p53 deletions or mutations can lead to cancer due to the inability of cells to respond to stress. The protein levels and post-translational modification state of p53 changes in response to cellular stress like DNA damage. Previous studies have shown that p53 can undergo coupled folding and binding with the E3 ubiquitin ligase, Mdm2, and the histone deacetylase, p300. In normal cells, p53 is kept at a low level by Mdm2, which marks it with ubiquitin, targeting p53 for proteasome …

Design Of A Comprehensive Modeling, Characterization, Rupture Risk Assessment And Visualization Pipeline For Abdominal Aortic Aneurysms, Golnaz Jalalahmadi

Theses

Abdominal aortic aneurysms (AAA) is a dilation of the abdominal aorta, typically within the infra-renal segment of the vessel that cause an expansion of at least 1.5 times the normal vessel diameter. It is becoming a leading cause of death in the United States and around the world, and consequentially, in 2009, the Society for Vascular Surgery (SVS) practice guidelines expressed the critical need to further investigate the factors associated with the risk of AAA rupture, along with potential treatment methods. For decades, the maximum diameter (Dmax) was introduced as the main parameter used to assess AAA behavior and its …

Lysenin Channels As Sensors For Ions And Molecules, Andrew Bogard, Gamid Abatchev, Zoe Hutchinson, Jason Ward, Pangaea W. Finn, Fulton Mckinney, Daniel Fologea

Physics Faculty Publications and Presentations

Lysenin is a pore-forming protein extracted from the earthworm Eisenia fetida, which inserts large conductance pores in artificial and natural lipid membranes containing sphingomyelin. Its cytolytic and hemolytic activity is rather indicative of a pore-forming toxin; however, lysenin channels present intricate regulatory features manifested as a reduction in conductance upon exposure to multivalent ions. Lysenin pores also present a large unobstructed channel, which enables the translocation of analytes, such as short DNA and peptide molecules, driven by electrochemical gradients. These important features of lysenin channels provide opportunities for using them as sensors for a large variety of applications. In …

Determination Of Vitamin D Metabolites And Their Epimers In Obese Emirati Population, Bashar Hiatham Alzohily

Theses

Vitamin D deficiency is a global issue, and despite all-year-round sunshine, it is a big problem in the UAE. Obesity or overweight refers to an accumulation of excessive fat that may harm health so, it is considered a worldwide health concern. The accurate measurement of vitamin D active forms is a problematic issue. This study aimed to develop and validate a UHPLC-MS/MS method for the new method for determining vitamin D levels in the obese Emirati population. The vitamin D epimers and other metabolites were determined by UHPLC-MS/MS system, model 8060. The methods include the collection of blood samples from …

The Role Of Emotional Intelligence In Chaplaincy: An Analysis If Ei In The Delivery Of Patient-Centered Care, Dean A. Darroux

Doctoral Dissertations and Projects

This study addressed the role of emotional intelligence among healthcare chaplains and its place in delivering patient-centered care at Adventist healthcare, Maryland. The study assumed that since healthcare chaplains’ journeys with their patients help them process their feelings as they understand the nature of their illness, they must identify their feelings and that of their patients to provide better care. It further examined the role of personality in determining its relationship with emotional intelligence and how it would influence patient-centered care. The literature surveyed indicated that emotional intelligence helps individuals understand their emotions as well as that of others. A …

Morphologic Remission Status Is Limited Compared To Δn Flow Cytometry: A Children's Oncology Group Aaml0531 Report., Lisa Eidenschink Brodersen, Robert B. Gerbing, M Laura Pardo, Todd A. Alonzo, Dana Paine, Wayne Fritschle, Fan-Chi Hsu, Jessica A. Pollard, Richard Aplenc, Samir B. Kahwash, Betsy Hirsch, Susana Ramondi, Denise Wells, E Anders Kolb, Alan S. Gamis, Soheil Meshinchi, Michael R. Loken

Manuscripts, Articles, Book Chapters and Other Papers

Risk stratification for acute myeloid leukemia (AML) uses molecular and cytogenetic abnormalities identified at diagnosis. Response to therapy informs risk, and morphology continues to be used more frequently than flow cytometry. Herein, the largest cohort of pediatric patients prospectively assessed for measurable residual disease (MRD) by flow cytometry (N = 784) is reported. The "difference from normal" (ΔN) technique was applied: 31% of all patients tested positive (AML range, 0.02% to 91%) after the first course of treatment on Children's Oncology Group study AAML0531. Detection of MRD following initial chemotherapy proved the strongest predicator of overall survival (OS) in univariable …

Chemical Synthesis Of Homogeneous Human E-Cadherin N-Linked Glycopeptides: Stereoselective Convergent Glycosylation And Chemoselective Solid-Phase Aspartylation, Chen Zeng, Bin Sun, Xuefeng Cao, Joshua Zhu, Olawale Micheal Oluwadahunsi, Ding Liu, He Zhu, Jiabin Zhang, Qing Zhang, Gaolan Zhang, Christopher Andrew Gibbons, Yunpeng Liu, Jun Zhou, Peng George Wang

Faculty Work Comprehensive List

We report herein an efficient chemical synthesis of homogeneous human E-cadherin N-linked glycopeptides consisting of a heptapeptide sequence adjacent to the Asn-633 N-glycosylation site with representative N-glycan structures, including a conserved trisaccharide, a core-fucosylated tetrasaccharide, and a complex-type biantennary octasaccharide. The key steps are a chemoselective on-resin aspartylation using a pseudoproline-containing peptide and stereoselective glycosylation using glycosyl fluororide as a donor. This synthetic strategy demonstrates potential utility in accessing a wide range of homogeneous N-linked glycopeptides for the examination of their biological function.