Digital Commons Network^™

A Novel Role For Rassf1a In The Regulation Of Ras Activation., Desmond Ramón Harrell Stewart

Electronic Theses and Dissertations

Ras is the most frequently activated oncogene in human cancer. It is not only the most frequently mutated oncogene, but is also rendered hyperactive in the wild-type form by aberrant regulation. Ras drives transformation and contributes to tumor aggressiveness by activating multiple downstream mitogenic effectors. Ras also possesses the paradoxical ability to induce apoptosis and senescence. Ras-induced apoptosis is not well understood, but has been largely attributed to the RASSF tumor suppressors, particularly RASSF1A. RASSF1A mediates Ras-induced apoptosis by activating pro-apoptotic proteins such as the MST kinases and BAX. RASSF1A is among the most frequently inactivated tumor suppressors in human …

Compassion Or Neoliberal Governance: Critiquing The Discourse Of Compassionate Louisville., Ekramul Islam

Electronic Theses and Dissertations

Since 2011, the city of Louisville, the site of this research, has been calling itself “Compassionate City.” The dissertation takes Compassionate Louisville as a city brand and seeks to investigate the effect of the image construction on the local politics. It aims to investigate the latent discourses produced by Compassionate Louisville in relation to the neoliberal political economy of the city. This dissertation employs the Critical Discourse Analysis (CDA) methodology. Compassionate Louisville, being a brand, produces a discourse of compassion. The CDA methodological approach helps to investigate this manufactured discourse in relation to the neoliberal socio-political context of the city. …

Willard Rouse Jillson And The Crystal Cave Of Sloans Valley, Kentucky, Gary A. O'Dell

Faculty Research at Morehead State University

No abstract provided.

Testing Flowering Perennial Plants In A Bermudagrass (Cynodon Spp.) Lawn, Paige Elizabeth Boyle, Michelle Wisdom

Horticulture Faculty Publications and Presentations

Most pollinating insects require a season-long succession of floral resources to fulfill life-cycle requirements. Incorporating forbs into turfgrass sites may create a season-long sequence of flowers to support foraging pollinators. However, persistence of forbs in warm-season turfgrasses such as bermudagrass (Cynodon spp.) may be affected by the competitive nature of the turfgrass and routine management practices such as mowing. A 2-year study was conducted to evaluate seven forbs (Bellis perennis L., Lotus corniculatus L., Prunella vulgaris L., Trifolium fragiferum L. ‘Fresa’, Trifolium repens L. ‘Durana’ and ‘Resolute’, Trifolium subterraneum L.) for persistence and ability to produce floral resources …

The Conservation Treatment And Technical Analysis Of A 20th Century Painting By Haitian Artist J.E. Gourgue, Melissa Amundsen

Art Conservation Master's Projects

This project describes the conservation treatment and technical analysis of a painting on Masonite with an unknown title by Haitian artist Jacques-Enguerrand Gourgue. Imaging and scientific analysis aided in the identification of the artist’s materials which informed the treatment of this fragile painting. Prior to treatment, the piece exhibited severe flaking, widespread losses, and loose paint fragments resting on the surface, requiring it to be stored horizontally. Following treatment, the painting is now in stable condition, can once again be exhibited vertically and is aesthetically suitable for display. Background research briefly discusses the artist’s background, affiliations, and imagery.

Symmetric Inverse Consistent Nonlinear Registration Driven By Mutual Information, Wen Fury, Keun Woo Park, Zhuhao Wu, Eunhee Kim, Moon-Sook Woo, Yu Bai, Lynn E Macdonald, Susan D Croll, Sunghee Cho

Faculty, Staff and Student Publications

BACKGROUND AND PURPOSE: Stroke is a major cause of chronic neurological disability. There is considerable interest in understanding how acute transcriptome changes evolve into subacute and chronic patterns that facilitate or limit spontaneous recovery. Here we mapped longitudinal changes in gene expression at multiple time points after stroke in mice out to 6 months.

METHODS: Adult C57BL/6 mice were subjected to transient middle cerebral artery occlusion. Longitudinal transcriptome levels were measured at 10 time points after stroke from acute to recovery phases of ischemic stroke. Localization and the number of mononuclear phagocytes were determined in the postischemic brain. Whole-mount brain …

The Role Of Music In Personal Therapy In Advanced Music Therapy Training: A Self-Inquiry, Jan Mark Casco

Theses & Dissertations

The purpose of this study is to explore the role of music through the similarities and differences of an Analytical Music Therapy (AMT) and Nordoff-Robbins Music Therapy (NRMT) trainee. The study employed a first-person research design and thematic analysis to assess three AMT and three NRMT personal therapy sessions. Music as well as the verbal interactions during the music therapy sessions were transcribed and analyzed. Five themes were constructed from the data, three similar themes and two different themes: (1) music as a place of meeting and the foundation of the therapeutic relationship, (2) using the voice to strengthen the …

Punishment In Prison: Constituting The "Normal" And The "Atypical" In Solitary And Other Forms Of Confinement, Judith Resnik, Hirsa Amin, Sophie Angelis, Megan Hauptman, Laura Kokotailo, Aseem Mehta, Madeline Silva, Tor Tarantola, Meredith Wheeler

Northwestern University Law Review

What aspects of human liberty does incarceration impinge? A remarkable group of Black and white prisoners, most of whom had little formal education and no resources, raised that question in the 1960s and 1970s. Incarcerated individuals asked judges for relief from corporal punishment; radical food deprivations; strip cells; solitary confinement in dark cells; prohibitions on bringing these claims to courts, on religious observance, and on receiving reading materials; and from transfers to long- term isolation and to higher security levels.

Judges concluded that some facets of prison that were once ordinary features of incarceration, such as racial segregation, rampant violence, …

Role Of Oxidative Stress And Altered Thyroid Hormones In Unexplained Infertility, Rehana Rehman, Hiba Ismail Rajpar, Mussarat Ashraf, Najeeha Talat Iqbal, Salima Lalani, Faiza Alam

Department of Biological & Biomedical Sciences

Objective: To explore the link between altered thyroid profile and oxidative stress marker in females with unexplained infertility.
Methods: The cross-sectional case-control study was carried out at the Islamabad Clinic Serving Infertile Couples, Islamabad, Pakistan, from June 2016 to August 2017, and comprised women aged 18-40 years regardless of ethnic background who were divided into two groups; those with unexplained infertility were the cases, while fertile women acted as the controls. Serum was analysed for triiodothyronine, thyroxine and thyroid stimulating hormone as well as for oxidative stress markers including manganese superoxide dismutase, glutathione reductase and adrenaline using enzymelinked immunosorbent assay. …

Analyzing The Management Practices Of East Texas Beef Cattle Producers, Hiliary Cheyenne Swor

Electronic Theses and Dissertations

A survey of East Texas beef cattle producers was designed to evaluate the management practices that were prevalent in the area. Demographic and cattle operation data from 103 respondents were analyzed using SPSS (Version 25; Chicago, IL). This electronic-based survey was distributed through Qualtrics. Respondents were located in Angelina, Nacogdoches, or Cherokee county. Most producers (38.8%) had a Bachelor’s degree. A negative correlation was established between castration method and weaning weight (p = - 0.204). Calves were weaned 50 pounds heavier when band or surgically castrated. Females were less likely to castrate their calves or utilize a mineral program. Approximately, …

A Novel Quantitative Real-Time Pcr Diagnostic Assay For Fecal And Nasal Swab Detection Of An Otariid Lungworm, Parafilaroides Decorus, Kalani M. Williams, M. K. Fessler, R. A. Bloomfield, William D. Sandke, Clara R. Malekshahi, Caroline D. Keroack, Pádraig J. Duignan, Samantha D. Torquato, Steven A. Williams

Biological Sciences: Faculty Publications

Parafilaroides decorus, also known as sea lion lungworm, is a metastrongyloid nematode that infects otariid hosts, such as the charismatic California sea lion, Zalophus californianus. P. decorus causes bronchointerstitial pneumonia, respiratory distress, reduced ability to swim, dive and hunt and as a result, increased mortality particularly in young animals. Respiratory disease is a leading cause of stranding and admission to rehabilitation centers on the Pacific coast. Low-coverage genomic sequencing of four P. decorus individuals analyzed through Galaxy's RepeatExplorer identified a novel repeat DNA family we employed to design a sensitive quantitative PCR (qPCR) assay for diagnosing infections from fecal or …

Studies On The Conversion Cyclic Aldehyde To Aromatic Amine By Reductive Amination, Sara Delfan

All Theses

Amines have broadly applied in the pharmaceutical and fine chemical industries, which cause intense research for developing efficient methods to produce primary, secondary and tertiary amines in organic synthesis. N, N-dimethyl tertiary amines are useful as catalysts which are buffers on the analysis of peptides and proteins. This transformation proceeds via an active C-N bond, and the most attractive method to produce amines is the reductive amination of carbonyl compounds. This method involves two steps, the condensation of aldehyde and the subsequent imine hydrogenation in the presence of a hydrogen donor. The synthesis of a catalytic, light-driven process for the …

Atitiudes Tow Ards Computer Games For Learning And Pl.A Yer Archetypes: An Exploration Of Measures On Preintervention Player Characteristics For Serious Game-Based Interventions, Benjamin Lee

Dissertations and Theses (Open Access)

Serious game-based interventions offer promising health outcome results with the aid of pre-intervention player tailoring and the development of measurements that evaluate pre-intervention player characteristics and subgroups. Video gaming measures can potentially provide helpful tailoring information that discerns individual video gaming preferences which could influence their overall user experience. It is critical that measures that target adolescent video gaming be psychometrically validated. There is a gap in the literature with psychometrically validated measures evaluating adolescent attitudes towards computer games for learning and gaming archetypes. Therefore the aims of this dissertation were to 1) evaluate the psychometric properties (i.e., reliability and …

Pathogens, Endosymbionts, And Blood-Meal Sources Of Host-Seeking Ticks In The Fast-Changing Maasai Mara Wildlife Ecosystem, Joseph Wang’Ang’A Oundo, Jandouwe Villinger, Maamun Jeneby, George Ong’Amo, Moses Yongo Otiende, Edward Edmond Makhulu, Ali Abdulahi Musa, Daniel Obado Ouso, Lillian Wambua

All Peer-Reviewed Publications

The role of questing ticks in the epidemiology of tick-borne diseases in Kenya’s Maasai Mara National Reserve (MMNR), an ecosystem with intensified human-wildlife-livestock interactions, remains poorly understood. We surveyed the diversity of questing ticks, their blood-meal hosts, and tick-borne pathogens to understand potential effects on human and livestock health. By flagging and hand-picking from vegetation in 25 localities, we collected 1,465 host-seeking ticks, mostly Rhipicephalus and Amblyomma species identified by morphology and molecular analysis. We used PCR with high-resolution melting (HRM) analysis and sequencing to identify Anaplasma, Babesia, Coxiella, Ehrlichia, Rickettsia, and Theileria pathogens and blood-meal remnants in 231 tick …

Small Stem Assay For Chestnut Blight Resistance In Segregating Half-Sib And Full-Sib Families Of F2 And Backcross Hybrid Chestnut Trees, Margaret Joyce Miller

Masters Theses and Doctoral Dissertations

Chestnut breeders routinely screen hybrid chestnut seedling progeny for resistance to chestnut blight in an orchard setting – a process that takes five to seven years. A novel technique that allows for screening in the first and second growing season is known as a small stem assay. We screened 110 first-backcross seedlings and 98 third-backcross seedlings in a completely randomized design in 2017 (Gentner 2018). In 2018, an additional 391 F2 hybrid chestnut seedlings were screened in a randomized complete block design. All seedlings were inoculated with the chestnut blight causal organism Cryphonectria parasitica when stem diameters were greater than …

Boron In The Pariette Wetlands, Uinta Basin, Ut, Palak Vasudeva

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Boron is a naturally occurring mineral in shale and coal beds formed in marine environments, as found in the Uinta Basin. Mining activity and the application of excess irrigation water on agricultural lands in the Pariette watershed lead to mobilization of B via surface run off. Water quality monitoring records from 2006- 2009 reported violations of Utah B standard for irrigation water 43-100% of the time, for water flowing through the Pariette Wetlands. This study aims to determine B distribution in abiotic (water, sediments) and biotic samples (macroinvertebrates, aquatic vegetation, fish, bird eggs), and to establish correlations between B concentrations …

Effect Of Plant Derived Tannins On Nitrogen And Carbon Cycling In Pasture Soils, Kathryn A. Slebodnik

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Pasture-finished beef has become increasingly popular, but nitrogen losses from these pastures are of concern. Legumes containing condensed tannins such as birdsfoot trefoil (Lotus corniculatus) and sainfoin (Onobrychis viciifolia) may serve as environmentally and economically viable alternative forages in pasture finishing systems while reducing soil nitrogen loss. The goal of this project was to understand how tannin type and concentration affects soil nitrogen cycling both in the lab and the field. This thesis: 1) compared the physical, chemical, and biological characteristics of soil samples obtained from grazed grass and tannin and non-tannin containing legume pastures, 2) …

Novel Three-Dimensional Scaffold For Skeletal Muscle Tissue, Matthew H. Clegg

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Cells are traditionally grown on a flat, plastic surface that has been optimized for cell attachment. While this is appropriate for growing and maintaining cells, this two-dimensional cell culture model is not an adequate representation of a three-dimensional body. Creating three-dimensional lab models is an important step to model diseases and damage to the skeletal muscle system.

To create this three-dimensional environment, a plastic frame was designed to support silkworm fibers. Skeletal muscle cells were then grown along the silkworm fibers and the growth of these cells in a three-dimensional environment was compared to cells grown on a flat, two-dimensional …

Cross-Boundary Stewardship For Wetland Integrity And Resilience In The Greater Rocky Mountain National Park Ecosystem, Meghan K. Tait

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Approximately half of the wetlands in Rocky Mountain National Park (RMNP) are degraded due to human disturbances that often occur beyond park boundaries. Like most protected areas, RMNP is part of a larger ecosystem with critical connections to surrounding lands. Therefore, more effective stewardship of wetlands within RMNP is likely to be achieved through cross-boundary cooperative efforts. Through interviews with wetland stewardship agencies and organizations and an analysis of their wetland plans and policies, barriers and opportunities for cross-boundary stewardship were identified, as well as common structures used to facilitate work across boundaries. Wetlands outside of RMNP are experiencing similar …

The Effects Of Different Organic Pastures On Dairy Heifer Growth And Development, Jacob A. Hadfield

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Raising dairy heifers in a certified organic setting can be difficult for producers. Conventionally, heifers are raised in a confined setting, and fed a total mixed ration (TMR) that is balanced daily to contain all the needed nutrients for developing heifers. Organic producers can use a TMR in their operations, but due to high organic feed costs, many choose to raise their heifers in pasture-based systems. While pasture-based systems may lower costs, heifers on pasture commonly have lower rates of gain, which can be financially burdensome to producers. Grass-legume pastures may help improve rates of gain in heifers on pasture-based …

New World Mammarenaviral Hemorrhagic Fever Virus Determinants Of Infection And Disease, Brady T. Hickerson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Several New World mammarenaviruses (NWM) are able to cause life-threatening hemorrhagic disease in humans. These agents use human transferrin receptor 1 (hTfR1), the main protein involved in cellular uptake of iron, to infect cells. Furthermore, use of hTfR1 correlates with the ability of these viruses to cause severe disease in humans. The most prevalent pathogenic NWM is Jun√≠n virus (JUNV), which is the etiological agent of Argentine hemorrhagic fever (AHF).The pathogenesis of AHF is poorly understood. A cardinal feature of severe cases of AHF is a prolonged increase of serum interferon-a (IFN-a) concentration. This increase is hypothesized to play a …

Influence Of Forage Diversity And Condensed Tannins On Livestock Foraging Behavior, Production And Environmental Impact, Sebastian P. Lagrange

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Eating a combination of forages with different chemistries (i.e., nutrients, beneficial compounds such as tannins) may enhance ruminant nutrition and reduce environmental impacts relative to eating single forages. I explored the influence of offering sheep and cattle all possible combinations of tanniferous (i.e., plants with tannins; birdsfoot trefoil, sainfoin) and non-tanniferous legumes (i.e., plants without tannins; alfalfa) or their monocultures on animal performance, behavior, and methane and nitrogen (N) emissions. Offering choices among these legumes to penned sheep improved intake and diet digestibility relative to feeding monocultures. Mixtures selected by sheep were better digested than mixtures containing equal parts of …

Soil Health Assessment On Arid Rangeland Soils Impacted By Oil And Gas Exploration, Development, And Extraction, Justin Allred

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Oil and gas well pad reclamation in arid environments such as in the Uinta Basin of Utah, presents unique challenges, including remote locations, limited water, and elevated soil salinity and sodicity. Successfully reclaimed Plugged and Abandoned (P&A) well pads should resemble the surrounding rangeland once fully reclaimed. Revegetation of native species is the primary indicator of successful reclamation, but the lack of water makes it challenging to re-seed native plants, while trying to prevent the encroachment of invasive plant species such as Bromus tectorum (cheatgrass), Salsola tragus (Russian thistle), and Halogeton glomeratus (halogeton). Could successful reclamation be reflective of good …

An Economic Comparison Of Two Leading Aquaponic Technologies Using Cost Benefit Analysis: The Coupled And Decoupled Systems, Grace M. Gibbons

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Aquaponics is an agricultural production practice which combines aquaculture, the raising of fish, and hydroponics, a soil-less crop production method, to create a system that symbiotically produces both. Compared to conventional open-field agriculture, this practice is highly resource efficient in terms of water and land usage, but has yet to be widely adopted due to a lack of research regarding its economic feasibility. This study compares the two most economically promising aquaponic system designs to determine which would be more profitable at the intermediate-commercial scale. The first of these is the coupled system which continuously circulates water in a closed …

Improving Our Ability To Estimate Vital Rates Of Endangered Fishes On The San Juan River Using Novel Applications Of Pit-Tag Technology, J. Benjamin Stout

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Estimating demographic parameters, such as survival and abundance, with accuracy and precision is vital for detecting trends in populations and assessing the effectiveness of management actions. In most cases, a lack of capture data make estimating parameters very challenging. The use of new technologies to increase the amount of remotely collected data is increasing, but brings new limitations and analytical issues to be resolved. One of those new technologies is the use of a mobile floating PIT-tag antenna to detect PIT-tagged fish. The issue that arises with this technology is determination of the status of detected tags (i.e., live fish …

Temporal Bisection Dynamics, Mahdi Shafiei

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Temporal bisection is a behavioral task used to study how we perceive time. However, it is not fully clear how time perception should be interpreted in different variations of this task. Moreover, it is not understood why the results of this task are often different for human and animal subjects. Understanding parameters of this task and making a connection between human and animal experiments may help researchers to understand how time is perceived in the brain and consequently disorders involving time perception.

In this thesis, I propose a computational model that A) provides researchers with a framework to study the …

Evaluation Of Enrichment And Identification Media For Isolation Of Common Bovine Salmonella Serotypes, Katie Irene Swanson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Salmonellosis is an important cause of diarrhea in humans and animals, which can lead to death in severe cases. Rapid and efficient detection of Salmonella spp. is essential to limit the spread of disease. Many enrichment procedures have been described for the isolation of Salmonella, and often include variations on Rappaport-Vassiliadis medium, which is recommended by the Food and Drug Administration (FDA). The current procedures vary in incubation temperature, incubation time, and type of media used for identification of Salmonella. This project compared four procedures for enrichment and identification of common bovine Salmonella serotypes. We determined the best …

Characterization Of Chinese Hamster Ovary Cell Performance With Pentanoic Acid And N-Acetyl Cysteine Treatment, Joseph Camire

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

To improve the cost of producing protein therapeutics manufacturers can attempt to increase the amount of protein produced per unit volume. Some small fatty-acids have shown the ability to increase protein concentrations in some manufacturing systems. An example system is in the industrially important cell line derived from a Chinese Hamster Ovary (CHO). A drawback of adding these fatty-acids is they can increase the rate of cell death.

A potential way to minimize the cell death rate, caused by adding fatty-acids, is to also add additional anti-oxidants. Through a series of studies, we demonstrate production with different fatty-acids, butanoic acid …

The Effects Of Gene Therapy In An Ovine Osteoarthritis Model, Crystal Collier

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Humans rely on the health of their joints for stability and mobility in daily life. In a normal, healthy functioning joint, complex processes maintain joint tissues, including remodeling, lubrication, and immune function among many other tasks. Osteoarthritis (OA) is the most common joint disorder in the U.S. It becomes most prevalent in adults 60+ years of age and causes decreased mobility, discomfort, and in some cases, excruciating pain. In the biological processes of osteoarthritis, the metalloproteinase enzymes responsible for the degeneration of cartilage are upregulated. The inhibitors of metalloproteinase activity are known as Tissue Inhibitor of Metalloproteinases (TIMPs), which keep …

Comparative Analysis Of Small Non-Coding Rna And Messenger Rna Expression In Somatic Cell Nuclear Transfer And In Vitro-Fertilized Bovine Embryos During Early Development Through The Maternal-To-Embryonic Transition, Jocelyn Marie Cuthbert

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Cloning animals using somatic cell nuclear transfer (scNT) was first successfully demonstrated with the birth of Dolly the sheep, but the process of cloning remains highly inefficient. By improving our understanding of the errors that may occur during cloned cattle embryo development, we could obtain a greater understanding of how specific molecular events contribute to successful development. The central dogma of biology refers to the process of DNA being transcribed into messenger RNA (mRNA) and the translation of mRNA into proteins, which ultimately carry out the functions encoded by genes. The epigenetic code is defined as the array of chemical …