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A Biophysical Investigation Of Stability, Ligand Binding, And Iron State Of Cyp102a1, Catherine A. Denning-Jannace

Theses and Dissertations--Chemistry

Cytochrome P450s (CYPs) are cysteine ligated Fe-heme monooxygenases that are found in all domains of life. In mammals, they have a role in xenobiotic metabolism and steroid synthesis, making them a fundamental requirement for survival. In addition, their ability to perform a variety of chemical reactions on an array of substrates makes CYPs highly sought for biotechnical applications such as wastewater remediation, production of potential drug candidates, and creation of drug metabolites. By mutating specific amino acids, these enzymes can be engineered to change their substrate binding profiles and achieve stereo- and regio-specific chemistry. While these mutations are essential to …

New Single Molecule In Vivo And Ex Vivo Methods To Understand The Physiological Effects Of Nicotine Use, Xu Fu

Theses and Dissertations--Chemistry

The consumption of tobacco products leads to several health risks, including respiratory diseases, cardiovascular diseases, and many different cancers. Nicotine, the primary addictive compound in tobacco, acts on the cholinergic system through nicotinic acetylcholine receptors (nAChRs), which is believed to be an essential component of addiction. However, the lack of a complete understanding of the neurobiological mechanisms of nicotine abuse is one of the primary barriers to finding potent therapeutics for smoking cessation. Single-molecule imaging is an ideal tool to study molecular mechanisms of protein and physiological changes both in vitro and in vivo. In this dissertation, our research …

Mass Grave Localization Prediction With Geographical Information Systems In Guatemala And Future Impacts, Perla Santillan

Master of Science in Forensic Science Directed Research Projects

Conducting physical searches for mass grave locations based on anecdotal evidence is a time consuming and resource intensive endeavor in circumstances that often pose a threat to personal safety. The development of tools and procedures to speed such searches can greatly reduce the risk involved, increase the number of individuals whose remains are recovered and identified; and, more importantly, reunite these remains with their loved ones to provide them with a proper burial. Geographic information systems (GIS) software, which can analyze and manipulate the spatial characteristics of known mass grave data, represents a powerful tool that can be used to …

Local Control Of Resource Allocation Is Sufficient To Model Optimal Dynamics In Syntrophic Systems, Glenn Ledder, Sabrina E. Russo, Erik B. Muller, Angela Peace, Roger M. Nisbet

School of Biological Sciences: Faculty Publications

Syntrophic systems are common in nature and include forms of obligate mutualisms in which each participating organism or component of an organism obtains from the other an essential nutrient or metabolic product that it cannot provide for itself. Models of how these complementary resources are allocated between partners often assume optimal behavior, but whether mechanisms enabling global control exist in syntrophic systems, and what form they might take, is unknown. Recognizing that growth of plant organs that supply complementary resources, like roots and shoots, can occur autonomously, we present a theory of plant growth in which rootshoot allocation is determined …

Predator-Dependent Functional Responses Alter The Coexistence And Indirect Effects Among Prey That Share A Predator, Kyle E. Coblentz, John P. Delong

School of Biological Sciences: Faculty Publications

Predator functional responses describe predator feeding rates as a function of prey abundance and are central to pred-ator–prey theory. Despite ample evidence that functional responses also depend on predator abundance, theory incor-porating predator-dependent functional responses has focused almost exclusively on specialist predator–prey pairs or linear food chains. This leaves a large gap in our knowledge as many predators feed on multiple prey, and in so doing, generate indirect effects among prey that can alter their coexistence. Here we investigate how predator-dependent functional responses in a one predator–two prey model alter the coexistence among prey and their net effects on one …

Adaptations Of Interferon Regulatory Factor 3 With Transition From Terrestrial To Aquatic Life, Monica Angeletti, Wan-Ling Nicole Hsu, Nashaat Majo, Hideaki Moriyama, Etsuko Moriyama, Luwen Zhang

School of Biological Sciences: Faculty Publications

Interferon regulatory factor 3 (IRF3) and IRF7 are closely related IRF members and the major factors for the induction of interferons, a key component in vertebrate innate immunity. However, there is limited knowledge regarding the evolution and adaptation of those IRFs to the environments. Two unique motifs in IRF3 and 7 were identified. One motif, GASSL, is highly conserved throughout the evolution of IRF3 and 7 and located in the signal response domain. Another motif, DPHK, is in the DNA-binding domain. The ancestral protein of IRF3 and 7 seemed to possess the DPHK motif. In the ray-finned fish lineage, while …

Wading In A Glycan Sea: Investigations Of, And Developing Bioinformatic Tools For, Carbohydrate Active Enzymes And Gene Clusters In Prokaryotes, Catherine Ann Ausland

Graduate Research Theses & Dissertations

Carbohydrates are immensely important biomolecule found in all organisms on earth, serving roles in structure, energy storage, protection and/or cell signaling. Carbohydrates are composed of an immense diversity of linkages, monosaccharide moieties and substitutions making them likely the most diverse biomolecule on earth, as well as the most abundant, being synthesized by plants and algae. Carbohydrates are synthesized and degraded by all organisms on earth through the expression of carbohydrate active enzymes, or ‘CAZymes’. In bacteria, CAZymes have been characterized to cluster in with transporters, regulators and/or other genes in so-called ‘polysaccharide utilization loci’, or PULs, to degrade carbohydrate substrates …

A Qualitative Study: Black Male College Students’ Perceptions Of Campus Law Enforcement Officers On A College Campus, Junelle M. Bennett

Graduate Research Theses & Dissertations

Black males are often the recipients of police brutality, criminal stereotyping, and inequitable treatment in higher education. This qualitative study examined the experiential narratives of 15 Black male college students’ perception towards law enforcement officers. The data was collected via one-on-one interviews, concluded with a focus group, and then presented in narrative forums. The participants’ feelings, attitudes, or beliefs about themselves as Black males significantly contributed to their inherent identification of the cultural challenges associated with law enforcement officers. The participants’ personal and vicarious life experiences prior to enrolling into higher education were significant to the authentic comprehension of the …

Vulnerability, Trauma, And Testimony In American Women’S Literature: A Long History, Jennifer L. Fife

Graduate Research Theses & Dissertations

This study examines the effects of vulnerability and trauma in American women’s literature across the intersections of genre, race, and time. In this dissertation I have applied a feminist long history approach to examine women’s literature from the seventeenth through twenty-first centuries together inspired by Mary Beard’s theory that compartmentalizing women’s history ignores long-range patterns and contributions. I have assembled the beginnings of a canon of women’s trauma literature that allows women writers to form a multi-century discourse community wherein trauma and recovery may occur. This analysis applies twentieth century medical research about trauma and recovery, particularly that of Judith …

The Influence Of The Type Of Supernatural And Paranormal Media On The Belief In The Various Forms Of Supernatural And Paranormal Phenomena, Mellissa Halsey

Graduate Research Theses & Dissertations

This thesis compares the consumption of the three different types of media: reality, live action fictional, and animated fictional, and the belief in the different types of supernatural and paranormal belief. The various phenomena addressed in this thesis were angels, demons, ghosts, psychics, aliens/UFOS, zombies, and Bigfoot. Models were created to test the belief level of each specific phenomena type against each of the three types of media, if enough media examples existed of each phenomena type. This type of analysis was used so that each media could be considered separately and then compared to each other. The hypotheses were …

Temporal Contiguity And Verbal Redundancy In Online Instruction: Examining Boundary Conditions For Multimedia Learning, Hal Hinderliter

Graduate Research Theses & Dissertations

The Cognitive Theory of Multimedia Learning has enjoyed a prominent role in guiding the development of online instruction, but recent research has proposed boundary conditions for some of its key principles. Moreno and Mayer advanced the possibility of a reverse redundancy effect when narration and fully redundant on-screen text are presented in a temporally contiguous manner. The current study investigated the interaction and main effects of temporal contiguity and verbal redundancy by randomly assigning university students to one of four narrated text-only online lectures. Existing knowledge of the topic of instruction, information literacy, was assessed using a 15-question selected-response instrument …

What Is Literature? An Investigation Of Definitions, Symptoms, And Routes Toward Literariness In English, Maxwell Robert Hoover

Graduate Research Theses & Dissertations

This project asks how and why certain works have received the label of “literature” while others have not. The project begins in Chapter 1 by examining seven glossaries of literary terms to define literature by means of understanding its parts, by which I mean the classes that literature has been divided into (e.g., genres). The overall finding of this chapter is that, while the glossarists seem to converge in their definitions of some terms, they greatly diverge in defining others. This divergence creates a problem in defining literature as a whole, because glossarists do not even reach a consensus in …

Liberalism And The Problem Of Compassion In Rousseau, Smith, And Tocqueville, Lewis Hoss

Graduate Research Theses & Dissertations

Liberal democracy has historically owed its attractiveness to the promise of a more compassionate society structured around pluralistic and tolerant principles. Yet recent events in western democracies seem to indicate a lack of compassionate social bonds. Why has a politics of compassion failed to materialize and what must be done to make good on the original promise of liberal democracy? To answer these questions, I provide a historically and theoretically grounded account of compassion as a principle of liberalism, with special attention to the political thought of Jean-Jacques Rousseau, Adam Smith, and Alexis de Tocqueville. In particular, I seek to …

Laminate-Like Behavior Of An Additively Manufactured Polymer With Underlying Bioinspired Architecture, Flavio Jonuzaj

Graduate Research Theses & Dissertations

Laminates are a class of composite materials in which several unidirectional laminae are stacked together to create a material with a clear layer-by-layer architecture. Traditionally, each lamina is itself a composite consisting of aligned fibers, such as cellulose, graphite, or glass, embedded within a polymer, ceramic, or metal host matrix. These materials are unique in the sense that their engineering properties can be greatly varied simply by changing the stacking order of lamina within the laminate. While there are several traditional manufacturing approaches for laminates, recent research has focused on assessing the feasibility of using additive manufacturing techniques to create …

Actin Regulation And Furrow Dynamics During Early Drosophila Embryogenesis, Yi Xie

Electronic Theses and Dissertations

Drosophila embryogenesis starts with a single nucleus undergo 13 rounds of nuclear divisions called syncytial cycles. Staring at cycle 10 when nuclei migrate to the surface of the embryo, massive and dynamic cortical actin structures and cleavage furrow formations occur. How actin regulators coordinate into an organized network directing three-dimension actin structures in the developing organisms is an unsolved question. Here, I present an in-depth characterization of actin cap dynamics: the actin caps go through expansion, stabilization, elongation and fragmentation phases in each cycle. Arp2/3 is the major contributor to actin cap formation. The functions of 7 different actin and …

Telling Stories, Healing Cultures: Feminist Healing Narratives By Contemporary American Women Of Color, Lindsay Marie Vreeland

Graduate Research Theses & Dissertations

Due to their experiences of oppression as a result of intersecting identities, women of Color are the most disenfranchised by Eurocentric patriarchal accounts and typically most in need of healing. As I explore in my research, works by women writers of Color—such as Louise Erdrich’s Four Souls, Sandra Cisneros’s Have You Seen Marie?, jia qing wilson-yang’s Small Beauty, and Toni Morrison’s Home and Paradise—gathered in this dissertation intentionally question and revise white settler versions of history that devalue people of Color; however, they also revise familiar stories and cultural traditions in their own communities. Doing so both empowers and enables …

Case Study: Examining Perceptions Of Students With Low Vision In Online Courses, Asma Fuad Marghalani

Graduate Research Theses & Dissertations

This qualitative case study explored the most helpful accommodations for students with low vision in online courses. Utilizing online surveys and individual interviews, this case study took place at a U.S. public university in the Midwest. The theoretical framework for this study was Universal Design for Learning (UDL). The findings indicate that students with low vision prefer receiving text and audio information. Additionally, the findings showed that extended time was the most helpful accommodation, alternative formats for materials, headings, and color-contrasting for online content were the most helpful accessibility aspects, and screen readers were the most helpful assistive technology. The …

Respiratory Sinus Arrhythmia, Behavioral Inhibition/Activation, And Behavioral Response To A Distressed Infant Simulator In Emerging Adults, Erin R. Mckay

Graduate Research Theses & Dissertations

Caregiver sensitivity and intrusiveness during infancy are predictive of the development of self-regulation, joint-attention, and cognitive ability. However, few studies have examined predictors of caregiver responses to infant distress. Of particular note is vagal tone, specifically respiratory sinus arrhythmia (RSA), a physiological measure of top-down self-regulation. Previous work has identified a relationship between RSA, as well as other measures of caregiver self-regulation, with caregiver sensitivity and intrusiveness. The current study also examined additional predictors of caregiver responsiveness, behavioral inhibition and activation, which was conceptualized as a bottom-up system of self-regulation due to its influence on motivation and personality. It was …

Virtual Faces In Virtual Spaces: Generating Social Capital In An Online World, Raymond James Miran

Graduate Research Theses & Dissertations

This thesis examines how online virtual reality games can generate social capital in the

social video game VRChat. Past research explores the generation of social capital from social

media and video games. These platforms generate social capital through different processes.

These differences are due to the technological differences and divergent purposes of the

platforms. VRChat bridges the computer-mediated-communication platforms as a social video

game. This thesis examines how VRChat as an online virtual reality generates bridging and

bonding online social capital by combining elements of the two platforms.

Results from 21 in-depth interviews showed that players of VRChat generated both …

Investigating The Behavioral Effects Of Juvenile Stress In The Prairie Vole Model, Marigny C. Normann

Graduate Research Theses & Dissertations

Early life stress during the juvenile period, such as emotional neglect, interpersonal difficulties, or other forms of non-violent maltreatment can have consequences into adulthood. Specifically, the negative effects include increased risk of psychiatric or physical illnesses, social deficits, and maladaptive behavioral responses to stress. Since these effects have far-reaching implications that can negatively alter later behavior and physiology, the present study assessed the effects of early life social stress on later social and affective behaviors in a social rodent species – the prairie vole. The prairie vole displays behavioral, cardiovascular, and neuroendocrine responses to social stress similar to those of …

Rational Design Of Memory-Based Sensors: The Case Of Molecular Calorimeters, Obianuju Augusta Nwokolo

Graduate Research Theses & Dissertations

Thermodynamic characterization is crucial for understanding molecular interactions. However, methodologies for measuring heat changes in small open systems are extremely limited. We document a new approach for designing molecular sensors that function as calorimeters: sensors based on memory. To design a memory-based sensor, we take advantage of the unique kinetic properties of nucleic acid scaffolds. Particularly, we elaborate on the differences in folding and unfolding rates in nucleic acid quadruplexes. DNA-based i-motifs unfold fast in response to small heats but do not fold back when the system is equilibrated with surroundings. We translated this behaviour into a molecular memory function …

A Bioinformatic Approach To Exploring Land Plant Evolution, Lauren Margaret Orton

Graduate Research Theses & Dissertations

Today, researchers are inundated with an overwhelming amount of data due to advances in DNA sequencing technologies. This has benefited the fields of both bioinformatics and phylogenomics substantially. With the ability to increase sampling at both the taxonomic and molecular levels, we are now able to produce robust phylogenomic reconstructions and better discern relationships between taxa.

The goals of this dissertation are in two distinct parts: 1) to advance the understanding of land plant terrestrialization and evolution through examination of the closest relative to the land plant lineage, a species of green algae; and 2) to explore evolution among a …

The Role Of Medial Septum Cholinergic Function In Processing Self-Movement Cues To Maintain Spatial Orientation, Jenna Rae Osterlund

Graduate Research Theses & Dissertations

Wandering is the most life-threatening and commonly reported symptom of Alzheimer’s Disease (AD), a neurodegenerative disorder characterized by an inability to maintain spatial orientation. The often deadly consequences of wandering are projected to rise in the coming decades due to the advancing aged population. These upcoming challenges necessitate a more comprehensive understanding of the neurobiology of spatial orientation in order to evaluate novel therapeutic techniques for symptoms such as wandering. The inability to maintain spatial orientation in AD may be due to the pathological degeneration of the hippocampal cholinergic system. This neurological system is conserved across species for its function …

Often Overlooked, But Not Forgotten: The Influence Of Demographic Measure Constraints On Participant Affect, Attitudes, Behavior, And Response Codability, Joy S. Pawirosetiko

Graduate Research Theses & Dissertations

Despite a growing number of recommendations varying in wording and response options, researchers have yet to agree on how to best request participant gender identity. Evidence suggests that gender binary and gender minority participants perceive gender identity measures differently, with gender binary participants potentially perceiving more gender inclusive measures as laborious and gender minority participants perceiving less gender inclusive measures as misgendering or invalidating. We sought to test whether seven versions of an online questionnaire (six experimental conditions with gender identity questions listed at the beginning of the questionnaire varying in gender inclusivity, and one control condition) significantly impacted participant …

A Framework For Heterologous Biosynthesis Of Natural Products In Mammalian Cells Via Polymer-Mediated Transfections, Logan Warriner

Theses and Dissertations--Chemical and Materials Engineering

With the promise to treat a multi-faceted list of serious inherited and acquired diseases, such as cancer, neurodegenerative and infectious diseases, and inherited genetic indications, gene therapy has continued to push the boundaries of traditional medicine since its earliest implementation. While much progress has been made, clinical success has largely remained elusive. Immunogenicity, difficulty producing commercially relevant quantities, and having a limited genetic payload still limits the ability of viruses to act as directed delivery agents for genetic material. As such, researchers have turned to cationic synthetic materials as a means of delivering nucleic acids, which can circumvent the immune …

A Thermodynamic And Feasibility Study Of Green Solvents For The Fabrication Of Water Treatment Membranes, Xiaobo Dong

Theses and Dissertations--Chemical and Materials Engineering

Nonsolvent induced phase separation (NIPS) has been widely used to fabricate polymeric membranes. In NIPS, a polymer is dissolved in a solvent to form a dope solution, which is then cast on a substrate and immersed in a nonsolvent bath, where phase inversion occurs. Petroleum-derived organic solvents, such as N-Methyl-2-Pyrrolidone (NMP) and Dimethylacetamide (DMAc), have been traditionally used to fabricate polymeric membranes via NIPS. However, these solvents may have negative impacts on environmental and human health; therefore, using greener and less toxic solvents, preferably derived from biomass, is of great interest to make membrane fabrication sustainable. In this dissertation, two …

Heart Tissue Reconstruction By Light-Controlled Cell Adhesion, Patterning And Alignment On Decellularized Scaffolds, Cong Li

Theses and Dissertations--Chemical and Materials Engineering

There is an urgent demand for engineered tissues and organs because of extremely limited donor tissues available for transplant. The challenge of tissue rejection has motivated many researchers to try to remove all of the cells from a heart and then add back in patient matched cells. These recellularized hearts are a promising avenue towards a functional bioartifical heart that is less likely to be rejected. Decellularized extracellular matrix (ECM) scaffolds generated from animal hearts do not have the cellular components that elicit immune rejection in patient, and they also preserve the native heart tissue structure. While decellularization techniques are …

First-Generation Black Males’ Challenges In Attending A Pwi: Understanding What Makes Them Persist, Shakeitra Simmons

Masters Theses

Using qualitative methodology, the researcher studied the challenges and persistence factors for undergraduate First-generation Black males to persist at a Predominantly White Institution. Through conducting six one-on-one semi-structured interviews, the researcher identified the challenges faced by this group of students as mental health, lack of support, and racism/microaggressions. The researcher also identified the factors for persistence at the PWI to be upward mobility, family and school personnel support, and campus involvement.

Narrativizing The Holidays: Event Branding Through Commodity Narrative Campaigns, Peighton Hinote

Masters Theses

Event planning and coordination often view events to be standalone, existing only until the event has passed. However, this thesis looks to serialize events to exist long after a single event takes place. Through the application of narrative, myth, and floating signification through the lens of semiotics theory, this thesis aims to create evergreen content for public relations practitioners in their efforts to promote events. The contents of this thesis focus on theory and application, allowing the two to enrich one another. Furthermore, this thesis practically applies foundational concepts from scholars like Fiske (1987, 1990) and Barthes (1957, 1970, 1978) …

Seasonal Variation In Mitochondrial Bioenergetics Of The Bluegill Sunfish, Lepomis Macrochirus, From A Shallow Midwest River, Derick Isaac Lamptey

Masters Theses

As average global temperature increase, the frequency and magnitude of extreme temperatures in shallow aquatic ecosystems are more ubiquitous. In order to understand how these changing thermal regimes affect aquatic ectotherms, it is essential to develop studies evaluating the response of ectotherms to seasonal fluctuating thermal regimes. Previous studies on fluctuating temperature regimes have reported an increased physiological stress leading to morphological, behavioral and biochemical adaptations. From the latter, the adaptive capacity and seasonal performance associated with optimal function of the oxidative phosphorylation system (OXPHOS) are key for species persistence. However, studies on this matter are scarce. This study explores …