Digital Commons Network^™

Lxr-Inverse Agonism Stimulates Immune-Mediated Tumor Destruction By Enhancing Cd8 T-Cell Activity In Triple Negative Breast Cancer, Katherine J. Carpenter, Aurore-Cecile Valfort, Nick Steinauer, Arindam Chatterjee, Suomia Abuirqeba, Shabnam Majidi, Monideepa Sengupta, Richard J. Di Paolo, Laurie P. Shornick, Jinsong Zhang, Colin A. Flaveny

Open Access Publications

No abstract provided.

Edematous Severe Acute Malnutrition Is Characterized By Hypomethylation Of Dna, Katharina V. Schulze, Mark J. Manary, Indi Trehan, Et Al.

Open Access Publications

No abstract provided.

Teg Platelet Mapping And Impedance Aggregometry To Predict Platelet Transfusion During Cardiopulmonary Bypass In Pediatric Patients, Erin E Barker, Arun Saini, Avihu Z Gazit, Susan M Shea, Sirine Baltagi, Brian F Gage, Philip C Spinella

Open Access Publications

No abstract provided.

A Targeted Gene Panel That Covers Coding, Non-Coding And Short Tandem Repeat Regions Improves The Diagnosis Of Patients With Neurodegenerative Diseases, Allen Chi-Shing Yu, Aldrin Kay-Yuen Yim, Anne Yin-Yan Chan, Liz Y P Yuen, Wing Chi Au, Timothy H T Cheng, Xiao Lin, Jing-Woei Li, Larry W L Chan, Vincent C T Mok, Ting-Fung Chan, Ho Yin Edwin Chan

Open Access Publications

Genetic testing for neurodegenerative diseases (NDs) is highly challenging because of genetic heterogeneity and overlapping manifestations. Targeted-gene panels (TGPs), coupled with next-generation sequencing (NGS), can facilitate the profiling of a large repertoire of ND-related genes. Due to the technical limitations inherent in NGS and TGPs, short tandem repeat (STR) variations are often ignored. However, STR expansions are known to cause such NDs as Huntington's disease and spinocerebellar ataxias type 3 (SCA3). Here, we studied the clinical utility of a custom-made TGP that targets 199 NDs and 311 ND-associated genes on 118 undiagnosed patients. At least one known or likely pathogenic …

Local Health Department Accreditation Is Associated With Organizational Supports For Evidence-Based Decision Making, Peg Allen, Stephanie Mazzucca, Renee G Parks, Mackenzie Robinson, Rachel G Tabak, Ross Brownson

Open Access Publications

No abstract provided.

Small Bowel Obstruction Secondary To Wild Banana Seed Ingestion, Divya K Natarajan, Phonexay Homthavong, Indi Trehan

Open Access Publications

No abstract provided.

A Brief Review Of Low-Dose Rate (Ldr) And High-Dose Rate (Hdr) Brachytherapy Boost For High-Risk Prostate, Benjamin W Fischer-Valuck, Hiram A Gay, Sagar Patel, Brian C Baumann, Jeff M Michalski

Open Access Publications

For patients with unfavorable or high-risk prostate cancer, dose escalated radiation therapy leads to improved progression free survival but attempts to deliver increased dose by external beam radiation therapy (EBRT) alone can be limited by late toxicities to nearby genitourinary and gastrointestinal organs at risk. Brachytherapy is a method to deliver dose escalation in conjunction with EBRT with a potentially improved late toxicity profile and improved prostate cancer related outcomes. At least three randomized controlled trials have demonstrated improved biochemical control with the addition of either low-dose rate (LDR) or high-dose rate (HDR) brachytherapy to EBRT, although only ASCENDE-RT compared …

Classification Of Human Ovarian Cancer Using Functional, Spectral, And Imaging Features Obtained From In Vivo Photoacoustic Imaging, Eghbal Amidi, Atahar Mostafa, Sreyankar Nandy, Guang Yang, William Middleton, Cary Siegel, Quing Zhu

Open Access Publications

No abstract provided.

Veliparib With First-Line Chemotherapy And As Maintenance Therapy In Ovarian Cancer, Robert L. Coleman, Andrea R. Hagemann, Et Al.

Open Access Publications

No abstract provided.

Structural And Functional Abnormities Of Amygdala And Prefrontal Cortex In Major Depressive Disorder With Suicide Attempts, Lifei Wang, Yimeng Zhao, Elliot K Edmiston, Fay Y Womer, Ran Zhang, Pengfei Zhao, Xiaowei Jiang, Feng Wu, Lingtao Kong, Yifang Zhou, Yanqing Tang, Shengnan Wei

Open Access Publications

Finding neural features of suicide attempts (SA) in major depressive disorder (MDD) may be helpful in preventing suicidal behavior. The ventral and medial prefrontal cortex (PFC), as well as the amygdala form a circuit implicated in emotion regulation and the pathogenesis of MDD. The aim of this study was to identify whether patients with MDD who had a history of SA show structural and functional connectivity abnormalities in the amygdala and PFC relative to MDD patients without a history of SA. We measured gray matter volume in the amygdala and PFC and amygdala-PFC functional connectivity using structural and functional magnetic …

Antibiotic Stewardship Teams And Clostridioides Difficile Practices In United States Hospitals: A National Survey In The Joint Commission Antibiotic Stewardship Standard Era, Valerie M Vaughn, M Todd Greene, David Ratz, Karen E Fowler, Sarah L Krein, Scott A Flanders, Erik R Dubberke, Sanjay Saint, Payal K Patel

Open Access Publications

OBJECTIVE: Clostridioides difficile infection (CDI) can be prevented through infection prevention practices and antibiotic stewardship. Diagnostic stewardship (ie, strategies to improve use of microbiological testing) can also improve antibiotic use. However, little is known about the use of such practices in US hospitals, especially after multidisciplinary stewardship programs became a requirement for US hospital accreditation in 2017. Thus, we surveyed US hospitals to assess antibiotic stewardship program composition, practices related to CDI, and diagnostic stewardship.

METHODS: Surveys were mailed to infection preventionists at 900 randomly sampled US hospitals between May and October 2017. Hospitals were surveyed on antibiotic stewardship programs; …

Regional Differences In The Expression Of Tetrodotoxin-Sensitive Inward Ca2+ And Outward Cs+ /K+ Currents In Mouse And Human Ventricles, Wei Wang, Rebecca L. Mellor, Jeanne M. Nerbonne, C. William Blake

Open Access Publications

No abstract provided.

Associations Between Family Weight-Based Teasing, Eating Pathology, And Psychosocial Functioning Among Adolescent Military Dependents, Arielle T Pearlman, Denise E Wilfley, Et Al

Open Access Publications

Weight-based teasing (WBT) by family members is commonly reported among youth and is associated with eating and mood-related psychopathology. Military dependents may be particularly vulnerable to family WBT and its sequelae due to factors associated with their parents' careers, such as weight and fitness standards and an emphasis on maintaining one's military appearance; however, no studies to date have examined family WBT and its associations within this population. Therefore, adolescent military dependents at-risk for adult obesity and binge-eating disorder were studied prior to entry in a weight gain prevention trial. Youth completed items from the Weight-Based Victimization Scale (to assess …

High Myc Mrna Expression Is More Clinically Relevant Than Myc Dna Amplification In Triple-Negative Breast Cancer, Eriko Katsuta, Li Yan, Takashi Takeshita, Kerry-Ann Mcdonald, Subhamoy Dasgupta, Mateusz Opyrchal, Kazuaki Takabe

Open Access Publications

DNA abnormalities are used in inclusion criteria of clinical trials for treatments with specific targeted molecules. MYC is one of the most powerful oncogenes and is known to be associated with triple-negative breast cancer (TNBC). Its DNA amplification is often part of the targeted DNA-sequencing panels under the assumption of reflecting upregulated signaling. However, it remains unclear if MYC DNA amplification is a surrogate of its upregulated signaling. Thus, we investigated the difference between MYC DNA amplification and mRNA high expression in TNBCs utilizing publicly available cohorts. MYC DNA amplified tumors were found to have various mRNA expression levels, suggesting …

Embracing Differences To Improve Success: American Orthopaedic Association Presidential Address, Boston, Massachusetts, June 23, 2017: Aoa Critical Issues, Regis J O'Keefe

Open Access Publications

No abstract provided.

Melanoma In Individuals With Neurofibromatosis Type 1: A Retrospective Study, May Zhang, Trisha Bhat, David H. Gutmann, Kimberly J. Johnson

Open Access Publications

No abstract provided.

Mission Critical Partnership For Infection Prevention Success, Karen Hoffmann, Hilary M Babcock

Open Access Publications

No abstract provided.

Protocol For The Effectiveness Of An Anesthesiology Control Tower System In Improving Perioperative Quality Metrics And Clinical Outcomes: The Tectonics Randomized, Pragmatic Trial, Christopher R King, Joanna Abraham, Thomas G Kannampallil, Bradley A Fritz, Arbi Ben Abdallah, Yixin Chen, Bernadette Henrichs, Mary Politi, Brian A Torres, Angela Mickle, Thaddeus P Budelier, Sherry Mckinnon, Stephen Gregory, Sachin Kheterpal, Troy Wildes, Michael S Avidan, Tectonics Research Group

Open Access Publications

No abstract provided.

Dupilumab Improves Symptoms, Quality Of Life, And Productivity In Uncontrolled Persistent Asthma, Jonathan Corren, Mario Castro, Pascal Chanez, Leonardo Fabbri, Vijay N Joish, Nikhil Amin, Neil M H Graham, Vera Mastey, Adeline Abbé, Christine Taniou, Puneet Mahajan, Ariel Teper, Gianluca Pirozzi, Laurent Eckert

Open Access Publications

BACKGROUND: In a pivotal, phase 2b study (NCT01854047) in patients with uncontrolled persistent asthma, despite using medium-to-high-dose inhaled corticosteroids plus long-acting β2 agonists, dupilumab improved lung function, reduced severe exacerbations, and showed an acceptable safety profile.

OBJECTIVE: To assess the impact of dupilumab on asthma control, symptoms, quality of life (QoL), and productivity.

METHODS: Data are shown for the intention-to-treat population receiving dupilumab 200/300 mg every 2 weeks (doses being assessed in phase 3; NCT02414854), or placebo. Predefined analyses of total scores were conducted at week 24 for the 5-item Asthma Control Questionnaire (ACQ-5), patient-reported morning/evening (AM/PM) asthma symptoms, Asthma …

Nanotherapy Targeting Nf-Κb Attenuates Acute Pain After Joint Injury, Huimin Yan, Xin Duan, Kelsey H Collins, Luke E Springer, Farshid Guilak, Samuel A Wickline, M Farooq Rai, Hua Pan, Christine T N Pham

Open Access Publications

Inflammation after joint injury leads to joint responses that result in eventual osteoarthritis development. Blockade of inflammation, by suppressing NF-κB expression, has been shown to reduce joint injury-induced chondrocyte apoptosis and reactive synovitis

Validation Of A Commercial Antibody To Detect Endogenous Human Nicastrin By Immunoblot, Rosana A Mesa, Elisha D O Roberson

Open Access Publications

Nicastrin (NCSTN) is a transmembrane glycoprotein that is part of the gamma-secretase complex. Gamma-secretase is a protease complex that cleaves type-I single-pass transmembrane proteins. There are many potential substrates for this complex, including NOTCH receptors and amyloid precursor proteins (APP). There are a number of commercial antibodies to nicastrin, but they do not agree on expected peptide size. We confirmed the specificity of a C-terminal binding rabbit anti-human antibody from Sigma-Aldrich (#N1660) using wildtype HEK293 cells and HEK293 cells deleted for nicastrin. The wildtype cells showed a prominent band at approximately 110 kDa. We confirmed this larger than expected sized …

Management Of Adrenal Insufficiency Risk After Long-Term Systemic Glucocorticoid Therapy In Duchenne Muscular Dystrophy: Clinical Practice Recommendations, Sasigarn A. Bowden, Anne M. Connolly, Kathi Kinnett, Philip S. Zeitler

Open Access Publications

No abstract provided.

Associations Of Mitochondrial And Nuclear Mitochondrial Variants And Genes With Seven Metabolic Traits, Aldi T. Kraja, Charles Gu, Mary F. Feitosa, Shiow J. Lin, Lihua Wang, Mary J. Wojczynski, Ruteja A. Barve, Ping An, Thomas J. Baranski, E. Warwick Daw, Lisa De Las Fuentes, Latisha D. Love-Gregory, Christine Williams, Wei Yang, D. C. Rao, Victor G. Davila-Roman, Michael Province, Et Al

Open Access Publications

No abstract provided.

Glioblastoma Treated With Magnetic Resonance Imaging-Guided Laser Interstitial Thermal Therapy: Safety, Efficacy, And Outcomes, Ashwin A. Kamath, Daniel D. Friedman, S Hassan A. Akbari, Albert H. Kim, Yu Tao, Jinqin Luo, Eric C. Leuthardt

Open Access Publications

BACKGROUND: Despite the multitude of available treatments, glioblastoma (GBM) remains an aggressive and uniformly fatal tumor. Laser interstitial thermal therapy (LITT) is a novel, minimally invasive treatment that holds promise for treating patients with GBM who are not candidates for traditional open craniotomy. However, due to the recent introduction of LITT into clinical practice, large series that evaluate safety and long-term outcomes after LITT are lacking.

OBJECTIVE: To present our institution's series of over 50 GBM patients treated with LITT, with regard to safety, efficacy, and outcomes.

METHODS: We performed a retrospective descriptive study of patients with histologically proven GBM …

Ctcf Variants In 39 Individuals With A Variable Neurodevelopmental Disorder Broaden The Mutational And Clinical Spectrum, Enrico D. H. Konrad, Marwan Shinawi, Emily Fassi, Et Al.

Open Access Publications

No abstract provided.

Syndemic Effects Of Hiv Risk Behaviours: Results From The Nhanes Study, L Smith, C Cao, X Zong, D T Mcdermott, S Stefanac, S Haider, S E Jackson, N Veronese, G F López-Sánchez, A Koyanagi, L Yang, I Grabovac

Open Access Publications

The aim of the present study is to use the syndemic framework to investigate the risk of contracting HIV in the US population. Cross-sectional analyses are from The National Health and Nutrition Examination Survey. We extracted and aggregated data on HIV antibody test, socio-demographic characteristics, alcohol use, drug use, depression, sexual behaviours and sexually transmitted diseases from cycle 2009-2010 to 2015-2016. We carried out weighted regression among young adults (20-39 years) and adults (40-59 years) separately. In total, 5230 men and 5794 women aged 20-59 years were included in the present analyses. In total, 0.8% men and 0.2% women were …

Recommendations And Alerting For Delirium Alleviation In Real-Time (Radar): Protocol For A Pilot Randomized Controlled Trial, Phillip E Vlisides, Jacqueline W Ragheb, Aleda Leis, Amanda Schoettinger, Kim Hickey, Amy Mckinney, Joseph Brooks, Mackenzie Zierau, Alexandra Norcott, Shirley Yang, Michael S Avidan, Lillian Min

Open Access Publications

No abstract provided.

Top 10 Research Priorities In Spatial Lifecourse Epidemiology, Peng Jia, Ross C. Brownson, Et Al.

Open Access Publications

No abstract provided.

Pain Symptomology, Functional Impact, And Treatment Of People With Neurofibromatosis Type 1, Frank D Buono, Lauretta E Grau, Matthew E Sprong, Kenneth L Morford, Kimberly J Johnson, David H Gutmann

Open Access Publications

Introduction: Neurofibromatosis type 1 (NF1) is a neurogenetic disorder affecting 1 in 3000 people worldwide, where individuals are prone to develop benign and malignant tumors. In addition, many people with NF1 complain of pain that limits their daily functioning. Due to the complexity of the disorder, there are few options for treating pain symptoms besides surgery and medications. Moreover, the spectrum of pain symptomatology and treatment, as well as the mechanisms underlying NF1-associated pain, has been understudied.

Methodology: To address this knowledge gap, we conducted a survey of 255 adults with NF1, leveraging the Washington University NF1 Patient Registry Initiative …

Long-Term Prospective Clinical And Radiographic Outcomes After Minimally Invasive Lateral Transiliac Sacroiliac Joint Fusion Using Triangular Titanium Implants, Peter G Whang, Emily Darr, S Craig Meyer, Don Kovalsky, Clay Frank, Harry Lockstadt, Robert Limoni, Andy J Redmond, Philip Ploska, Michael Oh, Abhineet Chowdhary, Daniel Cher, Travis Hillen

Open Access Publications

Background: Accumulating evidence supports the long-term safety and effectiveness of minimally invasive sacroiliac joint fusion (SIJF) for sacroiliac joint dysfunction.

Objective: To report 5-year clinical and radiographic follow-up in patients undergoing SIJF using triangular titanium implants (TTI).

Methods: One hundred and three subjects at 12 centers treated with SIJF using TTI in two prospective clinical trials (NCT01640353 and NCT01681004) were enrolled and followed in the current study (NCT02270203) with clinic visits at 3, 4 and 5 years. CT scans performed at 5 years were compared to prior CT scans (at 1 or 2 years) by an independent radiologist.

Results: Compared …