Digital Commons Network^™

The Development Of Intrinsically Cell-Permeable Peptide Libraries Using Mrna Display, Nicolas A. Abrigo

Theses and Dissertations

Peptides are emerging as promising therapeutics due to their inhibitory affinity towards protein-protein interactions (PPI). However, peptides have been limited mainly by their poor bio-stability and lack of cell permeability. Efforts to generate drug-like peptides have led to the development of macrocyclic peptides, which exhibit improved stability. Yet, most macrocyclic peptides still require the assistance of a cell penetrating peptide (CPP) for cellular entry.

High throughput technologies have been exceptional tools for the discovery of peptides to interrupt PPIs. This work details the recent advancements we have made to improve our high throughput technique, mRNA display, to yield more therapeutically …

An Investigation Of Neurological Soft Signs As A Discriminating Factor Between Veterans With Post-Traumatic Stress Disorder, Mild Traumatic Brain Injury, And Co-Occurring Post-Traumatic Stress Disorder And Mild Traumatic Brain Injury, David J. Rothman

Theses and Dissertations

While multiple Operation Enduring Freedom/Operation Iraqi Freedom/Operation New Dawn veterans suffer from mild Traumatic Brain Injury (mTBI), Post-traumatic Stress Disorder (PTSD), and co-morbid mTBI and PTSD, there remains difficulty disentangling the specific symptoms associated with each disorder using self-report and neurocognitive assessments. We propose that neurological soft signs (NSS), which are tasks associated with general neurologic compromise, may prove useful in this regard. Based on our review of the literature we hypothesized that individuals with PTSD would present with a greater number of NSS than controls or individuals with mTBI. Further, we hypothesized a synergistic effect, such that individuals with …

The Effect Of Music Characteristics On The Novelty And Usefulness Of Creative Ideas., Kathleen Keeler

Theses and Dissertations

This study explores the relationship between music and creativity. Prior research has conflicting results with some finding that music does influence creativity and some reporting no relationship and others finding that music is harmful to creativity. All of these studies, however, have largely focused on the presence vs. absence of music without consideration for the characteristics (i.e., musical key, tempo, etc.) that make up the sound we identify as music and their unique effects on us emotionally, physically, and cognitively. This dissertation contends that different characteristics of music influence different components of creativity (i.e., novelty and usefulness) through their effects …

Long-Term Effects Of Notch1 Signaling On Neural Stem Cells Following Traumatic Brain Injury, Cruz Sevilla Jr

Theses and Dissertations

Traumatic brain injury (TBI) is a devastating problem which stands as a leading cause of death and disability. The elderly is significantly affected by TBI, typically as the result of falls, and recovery is especially limited. This, in part, is associated with decreased tissue-specific stem cell regeneration and replacement of damaged cells in the aged brain. The diminished ability of the aged brain to recover is especially devastating after TBI, likely leading to permanent loss of sensory, motor, and cognitive functions. Studies have shown that the mature mammalian brain contains Neural Stem Cells (NSCs), found in specific regions of the …

The Analgesic-Like Properties Of Alcohol In Animal Models Of Chronic Pain, Bradley Neddenriep

Theses and Dissertations

Chronic pain and excessive alcohol consumption are individually problems in our society today. Alcohol Use Disorder (AUD) affects 15.1 million adult Americans each year. Chronic pain affects over 100 million people annually in the United States. However, there is growing evidence suggesting that these two conditions can often be interrelated with chronic pain increasing consumption of alcohol, and excessive alcohol consumption increasing pain that leaves a feedback cycle trapping millions of patients in an ever worsening spiral. Large population-based studies show an association between pain and alcohol abuse, suggesting a link between increased alcohol use and reduced pain. While rodent …

Untitled Unknown, Taylor Simone Stewart

Theses and Dissertations

This article is the first of a series exploring domination culture through the ways narrative has been indoctrinated as reality and weaponized as a holding cell for captives. Within this exploration, the narrative of domination is placed in relation to higher dimensional realms of the unknown; this being the before and after of domination culture. This positioning will allow for the reality of a simultaneous existence within the labyrinth of domination and a higher dimensional unknown to be framed. Within this series of articles, I question the roll of the rogue characters shamanistic agents of resisting domination, the fear of …

Nigga Is Historical: This Is Not An Invitation For White People To Say Nigga, Sandy Williams Iv

Theses and Dissertations

Over the past several years I have been on a quest to locate a world beyond the one I’ve been presented. I am interested in the history of atomic particles - like everything that radiates off of a monument (both literally and those things that are metaphorically reified) - invisible things, and the ways in which these things insect beyond our knowledge systems. This inquiry takes many forms. Mine is a conceptually based practice linked to record keeping and time, and the ways in which these concepts find plurality within our culture; or more pointedly, the importance that we attach …

The Puppets Look Like Flowers At Last, Evie Metz

Theses and Dissertations

The urge to uncover aspects of human condition permeates my work, from the fundamental curiosity of a child tearing apart their doll to uncover what lies within to continuing a quest in uncovering basic human urges through my puppet animated dramas and tragedies. There is a controversial line between the childlike and the adult-like that can be ambiguous, and at some times more discernible while other times less. I create handcrafted stop-frame puppet animations that explore self-conscious emotions such as embarrassment, shame, and envy within unpredictable life scenarios. These are animations about inner life, attempting to resolve conflicting elements of …

Au@Tio2 Nanocomposites Synthesized By X-Ray Radiolysis As Potential Radiosensitizers, Maria C. Molina Higgins

Theses and Dissertations

Radiosensitization is a novel targeted therapy strategy where chemical compounds are being explored to enhance the sensitivity of the tissue to the effects of ionizing radiation. Among the different radiosensitizers alternatives, nanomaterials have shown promising results by enhancing tumor injury through the production of free radicals and reactive oxygen species (ROS). In this work, Gold-supported titania (Au@TiO₂) nanocomposites were synthesized through an innovative strategy using X-ray irradiation, and their potential as radiosensitizers was investigated. Radiosensitization of Au@TiO₂ nanocomposites was assessed by monitoring the decomposition of Methylene Blue (MB) under X-ray irradiation in the presence of the nanomaterial. …

Parallel Narrative: Short-Video Social Media Platforms’ Influences On Contemporary Narrative, Ruiqi Zhang

Theses and Dissertations

Through the study of Kwai, a popular short-video social media platform in China, this thesis investigates the social issues, media class divides and aesthetics specific to Kwai culture. It further proposes a strategy of artistic practice - parallel narrative - an experiment in video art production and editing techniques that explores new possibilities of narrative in video art. Integrating theoretical research on Post-Internet art and object-oriented ontology, this thesis reveals people’s ability to digest multisource information and shows how mobile technologies and open-source materials contribute to the formation of parallel narratives.

Executive Functions As Moderators Of Response To Behavioral Interventions For Adolescents With Attention-Deficit/Hyperactivity Disorder, Stephen J. Molitor

Theses and Dissertations

Adolescents with Attention-Deficit/Hyperactivity Disorder (ADHD) experience significant academic impairment and multiple interventions have been developed to address common academic problems, such as difficulties with homework, organization, and planning skills. Given cost and resource constraints, research is needed on factors that mental health providers can use to select the most appropriate interventions. To date, there has been minimal research evaluating moderators of response to ADHD interventions. Further, many of the variables that have been studied do not have strong theoretical ties to the mechanisms of change invoked by the interventions. This study examined potential moderators of treatment response to two academic …

Group Decision-Making, Edward Cook

Theses and Dissertations

The present work explores improvements in group decision-making. It begins with a practical example using state-of-the-art techniques for a complex, high-risk decision. We show how these techniques can reveal a better alternative. Although we created an improved decision process, decision-makers were apt to protect their own organizations instead of the project. This tendency was reduced over the course of the decision-making process but inspired the first conceptual component of this work.

The first concept describes the “Cost of Conflict” that can arise in a group decision, using game theory to represent the non-cooperative approach and comparing the outcome to the …

The Role Of Tumor And Tumor Microenvironment On Breast Cancer-Associated Adipocyte Plasticity, Janina V. Pearce

Theses and Dissertations

Cancer-associated cachexia is a condition defined by a sustained net-negative energy imbalance. Although the different types of adipose tissue – white, beige, and brown – have been implicated in contributing to cancer-associated cachexia, the mechanisms of these maladaptive changes and their impact on whole-body energy expenditure have not been fully elucidated. Using breast cancer as our model, we demonstrate white adipose tissue browning in murine and human breast cancer; furthermore, we demonstrate that this effect is extremely localized and takes place early in tumor progression. We utilized in vitro cell culture techniques and demonstrate that cancer secreted factors and cross-talk …

Tone And Texture, Leather And Lace : A Case For Making Strong Choices In The Costume Design For The Three Musketeers., Emily L. Tappan

Theses and Dissertations

This thesis explores and describes key factors in the design process leading to production of The Three Musketeers by Catherine Bush. The document encompasses the justification and discussion of the choices made during the research, design, and production stages of developing the play for the stage, as well as impressions gained throughout the process to use in future design projects.

The Efficacy Of A Group Visual Art Bereavement Intervention With Older Adults, Rachel E. Weiskittle

Theses and Dissertations

Research on expressive art interventions for bereavement has burgeoned in recent years. Studies have supported their effectiveness in facilitating participants’ adjustment to loss (e.g., Rosner, Kruse, & Hagl, 2010; Uttley, 2015) and have revealed the frequency with which they are clinically implemented (Thompson & Neimeyer, 2014). Clinicians and recipients of expressive art interventions advocate for their helpfulness in grief processing (e.g., Gamino, 2015). Publications have highlighted particular visual art modules that facilitate adaptive adjustment to loss by providing avenues for self-expression, meaning making and continuing bonds with the deceased (Neimeyer, 2016), but few studies have quantitatively investigated whether they improve …

The Role Of Mindfulness In Self-View Investment: Neural And Subjective Indicators, Hadley Rahrig

Theses and Dissertations

Self-concept is strongly influenced by beliefs about one’s personal psychological attributes, and these beliefs are held with varying degrees of confidence and consequence. Hence, it is investment in self-views of those attributes that helps to regulate and maintain stable self-concept. Self-view investment is relevant to numerous self-related functions, but high self-view investment can also contribute to maladaptive self-views. Theory suggests that mindfulness cultivates a less personal, more objective perception of one’s thoughts, emotions and behaviors, and training in mindfulness has been shown to alter self-referential processing. The current pilot study (N=21) investigates the possible role of dispositional mindfulness in two …

Drawing As Language, Rebecca B. Whitson

Theses and Dissertations

All too often, the “I can’t draw” sentiment is believed by both the frustrated adolescent and adult alike. This is especially evident within the school environment. This paper aims to discuss how visual art --specifically drawing-- is structured, formed and expressed as a type of language, similar to a verbal, written, or physical one. This may give hope to even the most reluctant drawer that they can learn how to draw, opening another means of communication. An individual attains fluency when they are adept at drawing through the use of expression, technical, and observational skills, through practice and motivation, and …

The Role Of Cellular Aging In The Regulation Of Free Fatty Acid Induced Cellular Inflammation By Liver X Receptor Alpha, Charles S. Schwartz

Theses and Dissertations

Purpose: This study examined the impact of an acute bout of exercise on LXRα mRNA expression in isolated PBMCs from aerobically fit middle-aged males, and its associations with cellular inflammation. Furthermore, association between cellular age and LXRα were investigated. Methods: LXRα mRNA, plasma free fatty acids, IL-6, and MCP-1 responses were quantified following acute moderate intensity exercise and throughout recovery. To examine LXRα’s associations with cellular inflammation, PBMCs were stimulated with palmitate. Finally, telomere length was quantified as an indicator of cellular age. Results: LXRα mRNA expression was reduced at 90 minutes of recovery and did not influence systemic inflammation. …

Methods For Estimating The Optimal Time Lag In Longitudinal Mediation Analysis, Alicia Johns

Theses and Dissertations

Interest in mediation analysis has increased over time, with particular excitement in the social and behavioral sciences. A mediator is defined as an intermediate in the causal sequence between an independent and dependent variable. Previous research has demonstrated that the cross-sectional form of mediation analysis is inherently flawed, evidenced by the inability of the cross-sectional mediation model to account for temporal precedence and estimation of the indirect effect being biased in nearly all situations. For these reasons, a longitudinal model is recommended. However, a method for determining the exact time points to measure the variables used in mediation analysis has …

Associations Between Expressed Emotion, Mental Health, And Functioning In Families: Child Asthma Status As A Moderator, Katherine W. Dempster

Theses and Dissertations

Expressed emotion (EE), the affective attitudes and behaviors of one toward another, can affect caregivers’ behaviors toward their child. Research examining associations between EE and child/family outcomes is mixed; these associations may be affected by other influences such as the presence of a chronic disease or parent mental health. In this study of families living in an urban area, we examined associations between EE and child outcomes (anxiety/depressive symptoms) and family functioning, with parent anxiety as a covariate. We evaluated child asthma status as a moderator as the presence of a chronic illness may strengthen the association between EE and …

Response And Recovery Of Syntrophic And Methanogenic Activity To Saltwater Intrusion In A Tidal Freshwater Marsh Soil, David J. Berrier Jr.

Theses and Dissertations

Tidal freshwater wetland soils contain large amounts of organic carbon, some of which is mineralized to carbon dioxide (CO₂) and methane (CH₄) by a diverse consortium of anaerobic microorganisms that includes fermenters, syntrophs, and methanogens (MG). These microbial groups are tightly linked and often rely on cooperative interspecies metabolisms (i.e., syntrophy) to survive. Environmental perturbations can disrupt these interactions and thus alter the rates and pathways of carbon cycling. One environmental change of particular concern in coastal wetlands is sea level rise, which can result in increased episodic saltwater intrusion events into these ecosystems. These events …

Mother/Artist/Teacher: The Labor Of Becoming, Miranda L. Clayton

Theses and Dissertations

This paper explores possible applications of the experiential knowledge of motherhood in the field of art education through self-portraiture, a methodology which blends a/r/tography with elements of portraiture. A review of relevant literature situates the study within the scope of mothering pedagogy and arts-based research. Employing artistic practice and anecdotal journaling as the primary methods of inquiry, the researcher examines her experiences as a mother in a preservice art education program and the intersections of her roles as mother/artist/teacher. The researcher identifies resonant metaphors, including labor, separation, the umbilical cord, circles, the ovum, and pretending; offers parallels between mothering and …

Chemical Characterization Of Pseudognaphalium Obtusifolium By Gas Chromatography - Mass Spectrometry (Gc-Ms) To Assess Potential Therapeutic Phytochemicals And Toxicological Concerns Using Simulated Use Conditions, Regina Ballentine

Theses and Dissertations

Chemical Characterization of Pseudognaphalium obtusifolium by Gas Chromatography – Mass Spectrometry (GC-MS) to Assess Potential Therapeutic Phytochemicals and Toxicological Concerns Using Simulated Use Conditions

By Regina Ballentine

Virginia Commonwealth University, 2019

Director: Sarah C. Rutan, Professor, Department of Chemistry

Currently, there is an increasing demand for natural therapies and herbal products to treat various ailments. It is generally believed that natural therapies have fewer side-effects than traditional western medicine; however, they are often used in different strengths and formulations without consistency of the levels of target compounds or knowledge about toxicity. Due to this growing trend, a comprehensive chemical evaluation …

Differential Mobility Classifiers In The Non-Ideal Assembly, Thamir Alsharifi

Theses and Dissertations

The differential mobility classifier (DMC) is one of the core components in electrical mobility particle sizers for sizing sub-micrometer particles. Designing the DMC requires knowledge of the geometrical and constructional imperfection (or tolerance). Studying the effects of geometrical imperfection on the performance of the DMC is necessary to provide manufacturing tolerance and it helps to predict the performance of geometrically imperfect classifiers, as well as providing a calibration curve for the DMC. This thesis was accomplished via studying the cylindrical classifier and the parallel plate classifier. The numerical model was built using the most recent versions of COMSOL Multiphysics^{® …}

Material Interactions And Self-Assembly In Inkjet Printing, Karam Nashwan Al-Milaji

Theses and Dissertations

Inkjet printing has attracted much attention in recent years as a versatile manufacturing tool, suitable for printing functional materials. This facile, low-cost printing technique with high throughput and accuracy is considered promising for a wide range of applications including but not limited to optical and electronic devices, sensors, solar cells, biochips, and displays. The performance of such functional devices is significantly influenced by the deposit morphology and printing resolution. Therefore, fabrication functional devices with precise footprints by inkjet printing requires deep understanding of ink properties, material interactions, and material self-assembly.

In conventional inkjet printing process, where sessile droplets are directly …

Associations Between Ethnic-Racial Identity, Family Factors And Alcohol Problems Among Diverse Emerging Adults, Chloe Walker

Theses and Dissertations

The current study examined how multiple dimensions of ethnic-racial identity (ERI) were associated with alcohol use and alcohol use disorder (AUD) symptoms and how these relations varied by individuals’ ethnic-racial group among 1850 diverse emerging adults (M = 18.46, SD = .38). Further, measurement invariance of the Ethnic Identity Scale-Brief (EIS-B) was tested across Latinx, White, Black, Asian, and Multiracial students. Additionally, alternative models were examined that tested whether family factors (i.e., parent education and family history of alcohol problems) moderated the relations between ERI and alcohol problems to further examine nuances in these relations. Results indicated that the …

The Increased Frequency Of Micronuclei Seen In Women With A History Of Childhood Sexual Abuse Reflects More Numerical Than Structural Acquired Chromosomal Events: A Discordant Identical Co-Twin Study, Kaitlyn M. Dochelli

Theses and Dissertations

Childhood sexual abuse (CSA) is a stressful life experience with lasting/far-reaching health and psychopathological consequences. Our laboratory recently identified a significantly increased frequency of acquired chromosomal anomalies (assessed using the cytokinesis-blocked micronucleus assay) in adult female twins exposed to CSA when compared to their unexposed co-twin. The primary aim of this study was to evaluate potential mechanism(s) underlying the observed increases in levels of micronuclei in an expanded group of 90 female identical twins (61 CSA+ females and 29 CSA- females [including a total of 27 MZ co-twin pairs]) using fluorescence in situ hybridization (FISH) methodologies, with PNA probes specific …

An Enzymology And Inhibition Study Of A Camp-Dependent Protein Kinase Linked To Acth-Independent Cushing's Syndrome, Nicole Luzi

Theses and Dissertations

Cyclic-AMP dependent protein kinase (PKA) is a key intracellular signal transduction kinase that is modulated by G_s- and G_i-coupled GPCRs. Under normal physiological conditions, PKA exists as an inactive holoenzyme made up of two catalytic subunits and two regulatory subunits. Upon cAMP binding to the regulatory subunits, the catalytic subunits (PKACa) are released to perform various downstream phosphorylation events. However, aberrant PKA activation can cause various diseases including Cushing’s Syndrome, which is an endocrine disorder caused by the overproduction of cortisol by the hypothalamus-pituitary-adrenal hormone system. This disorder can be caused by pituitary adenomas that release …

Revealing The Human Resource Development Discourse: A Mixed Methods Study Of Similarities And Differences In Academic And Practitioner Language, Or Labels-In-Use, Holly D. Jackson

Theses and Dissertations

Woven within the pages of HRD’s historical literature, a variety of scholarly voices can be found drawing attention to the increasing inconsistency in the language of the field. Within the literature, we also find evidence of a long-standing discord and debate regarding the field’s definition and identified boundaries. This is the first study that attempts to elevate the conversation of HRD’s definition to that of an exploration of what is shared, and what makes the discipline’s members unique. Utilizing Li’s (2009) lens of disciplinary identity and elements of Gee’s (1999) theory of Discourse, this study presents a concept of what …

Targeting Sphingosine Kinase 2 As A Treatment For Cholangiocarcinoma, Anthony D. Stillman

Theses and Dissertations

Cholangiocarcinoma (CCA) has a high mortality rate and its occurrence is rising. This increase prompts the need for improved CCA treatments. Studies have suggested that CCA is highly reliant on the sphingosine-1-phosphate-receptor-2 (S1PR2) and sphingosine kinase 2 (SphK2). Recently, a competitive SphK2 inhibitor, ABC294640, has been approved for clinical trial. ABC294640 has the potential to treat CCA, which is support by a phase I clinical study that was able to temporarily treat a patient suffering from metastasized CCA with ABC294640. To determine the viability of ABC294640 as a treatment for CCA, this study focused on determining the effects of ABC294640 …