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2019

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Numerical Calculation Of Heat Transfer In Single Medium-Deep Geothermal Well, Shang Hongbo, Zhao Chunhu, Jin Dewu, Xu Shuanhai, Wang Xiaodong, Zhang Weidong, Han Yongliang Dec 2019

Numerical Calculation Of Heat Transfer In Single Medium-Deep Geothermal Well, Shang Hongbo, Zhao Chunhu, Jin Dewu, Xu Shuanhai, Wang Xiaodong, Zhang Weidong, Han Yongliang

Coal Geology & Exploration

Medium-deep geothermal single well heat transfer is a technology of developing geothermal energy to "obtain heat without extracting groundwater". This technology has the advantages of not damaging the groundwater environment and taking more heat. However, there are few studies on the numerical calculation of single well heat transfer above 3000m depth. In this paper, based on the geothermal geological conditions in Xi'an, the numerical method was used to calculate the heat transfer of a medium-deep geothermal single well with different well structures (L-shaped directional wells and cluster directional wells) under the scenario of continuous operation of a heating season. The …

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Fluid Factor Of Fracture And Seismic Velocity Response In Anisotropic Coal Beds, Li Qin, Ma Suibo, Zhao Bin Dec 2019

Fluid Factor Of Fracture And Seismic Velocity Response In Anisotropic Coal Beds, Li Qin, Ma Suibo, Zhao Bin

Coal Geology & Exploration

The storage and transport of gas and water in coal seam are related to the density, strike and filler of cracks. Research on the identification and prediction of coal seam cracks will be helpful to prevent and control disastrous accidents in coal mines, such as water inrush and gas outburst. It plays an important role in the safety evaluation of coal mining. Based on the theory of fracture equivalent model, the variation characteristics of fluid factor in coal seam crevices with different fillings and fracture weaknesses parameters(fracture density, aspect ratio, volume modulus, shear modulus, Lamé parameters) are analyzed by combining …

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Influencing Factors Of Azimuth Electromagnetic Wave Roof And Floor Detection While Drilling Along Coal Seam, Chen Gang, Fan Yiren, Li Quanxin Dec 2019

Influencing Factors Of Azimuth Electromagnetic Wave Roof And Floor Detection While Drilling Along Coal Seam, Chen Gang, Fan Yiren, Li Quanxin

Coal Geology & Exploration

Aiming at the problems of poor detection accuracy and low efficiency of roof and floor interface in horizontal well drilling in coal mine, the design idea of explosion-proof azimuth electromagnetic wave logging tool while drilling in coal mine is put forward. In order to suit the working environment of underground coal seam, the influence of instrument parameters such as source distance, transmitting frequency and coil installation angle on signal response was obtained by forward simulation. The results show that the received signal strength of the azimuth electromagnetic wave instrument while drilling increases with the increase of transmitting frequency, coil distance …

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Csamt Phase Correction And Its Application In Detection Of Water-Accumulating Area Of Goaf In Coal Mine, Su Chao, Hou Yanwei, Wang Cheng, Li Dan Dec 2019

Csamt Phase Correction And Its Application In Detection Of Water-Accumulating Area Of Goaf In Coal Mine, Su Chao, Hou Yanwei, Wang Cheng, Li Dan

Coal Geology & Exploration

Magnetic field data of controlled source audio magnetotelluric method(CSAMT) is vulnerable to electromagnetic interference, and will lead to serious degradation of phase data quality. The affected phase data are corrected by referring to the high quality phase data of adjacent measuring points and combining the least squares fitting and interpolation method. Through forward simulation analysis, it is found that cubic polynomial interpolation is effective, not only can restore the variation characteristics of phase curve more accurately, but also involve the corrected phase data in the inversion calculation, so that the inversion results are more accurate and reliable. The method is …

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Test Of Water Absorbent Resin For Disposal Of Waste Mud, Wei Meng, Qi Jinhui, Zhang Ningxin, Wen Jun Dec 2019

Test Of Water Absorbent Resin For Disposal Of Waste Mud, Wei Meng, Qi Jinhui, Zhang Ningxin, Wen Jun

Coal Geology & Exploration

In the course of engineering construction, a lot of abandoned mud is produced. How to properly handle these mud has received extensive attention. In this paper, the application of absorbent resin in waste mud treatment is studied by series of experiments. Compared with the common flocculants, the flocculation effect of absorbent resin is better, and the separation speed of waste mud solid liquid can be accelerated obviously. The water absorption rate of absorbent resin in the mud reaches 46.33 g/g, which can be incorporated into the mud to greatly reduce the water content and fluidity of the mud within 30 …

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Concealed Riprap Detection Technology In Pile Foundation Engineering, Li Junjie, Ge Longjin, Zhang Honggang, Li Jianmin, Zhu Honglei, Li Guanhao Dec 2019

Concealed Riprap Detection Technology In Pile Foundation Engineering, Li Junjie, Ge Longjin, Zhang Honggang, Li Jianmin, Zhu Honglei, Li Guanhao

Coal Geology & Exploration

It is often necessary to use pile foundation to deal with soft soil foundation for the newly built gate station near the seawall. If there is a concealed riprap layer in the construction area, it will affect the construction progress of pile foundation and increase the construction cost. Furthermore, the spatial distribution characteristics of riprap also play a decisive role in the selection of pile foundation construction technology. In view of the blindness problem in using the drilling method to investigate the irregular concealed riprap, we adopt the comprehensive investigation technology combining the ground penetrating radar with the high density …

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Fault Identification Technology Of Ant Attribute Optimization, Li Hongwei, Bai Xuelian, Cui Jingbin, Wan Zhonghong, Yuan Shihong, Chu Wanchang Dec 2019

Fault Identification Technology Of Ant Attribute Optimization, Li Hongwei, Bai Xuelian, Cui Jingbin, Wan Zhonghong, Yuan Shihong, Chu Wanchang

Coal Geology & Exploration

In the field of seismic geological exploration, the ant attributes is more and more widely used for rapid identification of fault system, and in the process of practical application, ant attributes based on 3D seismic data extraction are displayed either along strata slice(plan) or in section, the features of fault system and distribution characteristics of seismic events is not corresponding to the actual situation, the application effect is poorer, fault interpretation work is given priority to with manual interpretation backbone profile, unable to realize fault automatic tracking. In view of this widespread situation, this paper studies and analyzes the actual …

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Analysis Of Factors Affecting The Automatic High-Frequency Induction Brazing Strength Of Pdc, Dai Yongbo Dec 2019

Analysis Of Factors Affecting The Automatic High-Frequency Induction Brazing Strength Of Pdc, Dai Yongbo

Coal Geology & Exploration

With the development of automation and intelligentization in manufacturing industry, the research on PDC bit brazing automation is one of the key development directions of bit production technology. In order to study the influencing factors and adaptation methods of welding strength in PDC automatic brazing process, PDC high frequency induction welding and shear strength comparison test were carried out by using PDC and alloy pillar as the base material through special tooling and test conditions. Combined with the shear strength experiment and the morphology of brazing surface of PDC high-frequency induction brazing, the effect of brazing filler alloys, fluxes, surface …

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Combined Cbm Production Behavior And Development Strategy Of Multiple Coal Seams In Zhijin Block, Guo Tao, Gao Xiaokang, Meng Guixi, Xiao Cui Dec 2019

Combined Cbm Production Behavior And Development Strategy Of Multiple Coal Seams In Zhijin Block, Guo Tao, Gao Xiaokang, Meng Guixi, Xiao Cui

Coal Geology & Exploration

Aiming at the problems that the CBM layers are numerous and thin, it is difficult to develop it effectively in south China, on the basis of the actual production data of CBM wells in Zhijin block, the production characteristics and the influencing factors of gas production of the directional wells for co-extraction of different seams and the horizontal wells for thin seams were analyzed, and corresponding suggestions for CBM development were put forward. The analysis indicated that the gas production of co-extraction of different seams was quite different, was influenced by the liquid-supplying capacity of formation, the height of desorbing …

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Application Of High Precision Gravity Survey In Detecting Mined-Out Areas Of Fushun Coal Mine, Huan Hengfei, Gao Tie, Zhao Haiqing, Zhao Weijun, Guo Canwen, Shi Bin Dec 2019

Application Of High Precision Gravity Survey In Detecting Mined-Out Areas Of Fushun Coal Mine, Huan Hengfei, Gao Tie, Zhao Haiqing, Zhao Weijun, Guo Canwen, Shi Bin

Coal Geology & Exploration

In order to find out the location, depth and distribution range of the mined-out areas in Fushun coal mine, and comprehensively considering the factors affecting the geophysical methods in the cities, the method of high-precision gravity survey was firstly used to study the mined-out areas in the area. Firstly, by analyzing the physical differences of the mined-out areas, a theoretical model was established for forward calculation and preliminarily studied on applicability of the gravity method. Then, a large-scale work of high-precision gravity survey was arranged in the study area, and through potential field separation,3D apparent density inversion and boundary identification …

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Drainage Technology And Test Of Stratified Pressure Control With Four-Channels Of Cbm Well In Double Coal Seams, Pang Tao, Jiang Zaibing, Li Bingang, Du Xinfeng Dec 2019

Drainage Technology And Test Of Stratified Pressure Control With Four-Channels Of Cbm Well In Double Coal Seams, Pang Tao, Jiang Zaibing, Li Bingang, Du Xinfeng

Coal Geology & Exploration

Aiming at the problems of mutual interference between layers and serious damage in reservoirs in the production process of double-coal CBM wells, a four-channel stratified pressure control drainage technology was proposed, in the process of drainage, two coal seams were controlled in different pressure systems to achieve the purpose of stratified production and CBM well drainage device with four-channel packer, monitoring equipment, multi-channel tubing centralizer, roller sucker rod centralizer and auxiliary communication tube for double coal seams was developed. Field application test shows that the four-channel packer can achieve the isolation effect according to the needs, so that the two …

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Table Of Contents, The Editors Dec 2019

Table Of Contents, The Editors

Coal Geology & Exploration

No abstract provided.

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Research And Application Of Casing Milling And Fishing Technology In Floor Comb-Shaped Directional Borehole, Guan Qiangsheng, Wei Hongchao Dec 2019

Research And Application Of Casing Milling And Fishing Technology In Floor Comb-Shaped Directional Borehole, Guan Qiangsheng, Wei Hongchao

Coal Geology & Exploration

In order to solve the problem that using the conventional strong-force rotary drawing process for borehole sticking accident in comb-shaped directional borehole in floor is not ideal, and using reverse thread drill pipe or strong twist cause big time and economic loss, through the calculation and analysis of the passing capacity of casing milling and fishing pipe in the curved hole, the technical parameters of casing milling and fishing, the sticking accident of floor comb-shaped directional borehole with the depth of 344 m in a coal mine in Henan Province was successfully solved. The application results show that the maximum …

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Evaluation Of Coalbed Methane Drainage Effect For Multi-Target Seams In Jincheng Region, Yang Jianchao, Li Guihong, Liu Yuhui, Li Guofu, Wang Chaoshuai, Sun Tianyu Dec 2019

Evaluation Of Coalbed Methane Drainage Effect For Multi-Target Seams In Jincheng Region, Yang Jianchao, Li Guihong, Liu Yuhui, Li Guofu, Wang Chaoshuai, Sun Tianyu

Coal Geology & Exploration

The study of the multi-target seams drainage effect on coalbed methane wells has great value, including coal safe mining, underground gas control, determination of development technical indicators, single well production, rational division of development layer, efficient development of coalbed methane, and development of medium and long-term coalbed methane development planning. Taking Chengzhuang mine of Jincheng as an example, the sampling data of the extraction effect in the middle and late stages of development are compared with the original gas content of the adjacent wells to evaluate the extraction effect, and the main control factors affecting the drainage effect are analyzed …

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Supporting Technology Of Roadway Crossing Fractured Zone Of Fault Under Dynamic And Static Load, Song Weihua, Di Chunlei, Deng Zhaorui Dec 2019

Supporting Technology Of Roadway Crossing Fractured Zone Of Fault Under Dynamic And Static Load, Song Weihua, Di Chunlei, Deng Zhaorui

Coal Geology & Exploration

The gateway 5303 encountered multiple normal faults during the driving in Changping mine of Jinmei Group. For the purpose that the gateway can pass through the faults safely and rapidly and ensure applicative methods and scope of supporting. So based on the principle of maximum principal stress and fracture damage mechanics of rock, the paper analyzes the crushing mechanism of the normal fault zone under dynamic and static load from macroscopic and microscopic perspectives. Then, the plastic failure model of fault zone was established, the normal fault sliding mechanism and supporting theory were combined to derive the expression of the …

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Comprehensive Evaluation Technology And Application Of Grouting Reinforcement Effect For Broken Coal And Rock Mass, Wang Xiaolei, Ji Zhigang, Luo Wenqiang Dec 2019

Comprehensive Evaluation Technology And Application Of Grouting Reinforcement Effect For Broken Coal And Rock Mass, Wang Xiaolei, Ji Zhigang, Luo Wenqiang

Coal Geology & Exploration

Grouting reinforcement of broken coal and rock mass plays an important role in controlling the stability of surrounding rocks, and the grouting effect detection is an important link to ensure the grouting engineering to meet the requirements. The evaluation index was quantitatively analyzed by slurry filling rate method, mechanical parameter method, segmented water injection method, geological radar method, drilling television method and core inspection method. The comprehensive evaluation form of grouting effect was put forward, and the application research was carried out. The results show:The rate of filling slurry and coring was more than 90%; After grouting, the compressive strength …

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Deformation Characteristics And Support Timing Of Deep High Stress Roadway, Geng Weile, Dong Ziwen, Guo Shengli, Pi Zikun Dec 2019

Deformation Characteristics And Support Timing Of Deep High Stress Roadway, Geng Weile, Dong Ziwen, Guo Shengli, Pi Zikun

Coal Geology & Exploration

The rock occurrence environment and structural characteristics determine its strength criteria, and affects the mechanical and deformation characteristics of surrounding rock. This paper relies on the deep replacement project of a mine in Yunnan, based on the rock strength criteria, and analyzes the mechanics and deformation characteristics of surrounding rock and the reasonable secondary support time of roadway to decrease the influence of the roadway support effect because of the obvious rheological properties present under high ground stress. The results showed that the min-average simulated deviations mf was generalized H-B criterion which was aimed at the sum of deviation absolute …

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Hydrodynamic Characterization Of Mugla Karst Aquifer Using Correlation And Spectral Analyses On The Rainfall And Springs Water-Level Time Series, Çağdaş Sağır, Bedri Kurtuluş, Moumtaz Razack Dec 2019

Hydrodynamic Characterization Of Mugla Karst Aquifer Using Correlation And Spectral Analyses On The Rainfall And Springs Water-Level Time Series, Çağdaş Sağır, Bedri Kurtuluş, Moumtaz Razack

KIP Articles

Karst aquifers have been an important research topic for hydrologists for years. Due to their high storage capacity, karst aquifers are an important source of water for the environment. On the other hand, it is safety-critical because of its role in floods. Mugla Karst Aquifer (SW, Turkey) is the only major water-bearing formation in the close environs of Mugla city. Flooding in the wet season occurs every year in the recharge plains. The aquifer discharges by the seaside springs in the Akyaka district which is the main touristic point of interest in the area. Non-porous irregular internal structures make the …

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Is It T3 Thyrotoxicosis? A Case Of Falsely Elevated Tri-Iodothyronine (T3) Levels Leading To A Diagnosis Of Multiple Myeloma, Nanik Ram, Saira Furqan, Sibtain Ahmed Dec 2019

Is It T3 Thyrotoxicosis? A Case Of Falsely Elevated Tri-Iodothyronine (T3) Levels Leading To A Diagnosis Of Multiple Myeloma, Nanik Ram, Saira Furqan, Sibtain Ahmed

Section of Diabetes, Endocrinology and Metabolism

We are presenting a case of falsely elevated T3 levels in a patient due to interference from monoclonal immunoglobulins. A 56-year-old, clinically euthyroid man referred to the endocrinology clinic of the Aga Khan university, Karachi Pakistan, for possible T3 thyrotoxicosis after thyroid function tests revealed total T3 >12.32 nmol/L (reference range 0.6-2.79), normal TSH, and total T4 level. There was a mismatch in clinical and laboratory parameters and preliminary laboratory results were suggestive of thyroid binding globulin abnormalities. Further evaluation in this context unmasked multiple myeloma. The presence of monoclonal immunoglobulins can lead to assay interference and spurious results. To …

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Hydrodynamic Characterization Of Mugla Karst Aquifer Using Correlation And Spectral Analyses On The Rainfall And Springs Water-Level Time Series, Çağdaş Sağır, Bedri Kurtuluş, Moumtaz Razack Dec 2019

Hydrodynamic Characterization Of Mugla Karst Aquifer Using Correlation And Spectral Analyses On The Rainfall And Springs Water-Level Time Series, Çağdaş Sağır, Bedri Kurtuluş, Moumtaz Razack

KIP Articles

Karst aquifers have been an important research topic for hydrologists for years. Due to their high storage capacity, karst aquifers are an important source of water for the environment. On the other hand, it is safety-critical because of its role in floods. Mugla Karst Aquifer (SW, Turkey) is the only major water-bearing formation in the close environs of Mugla city. Flooding in the wet season occurs every year in the recharge plains. The aquifer discharges by the seaside springs in the Akyaka district which is the main touristic point of interest in the area. Non-porous irregular internal structures make the …

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Program Revitalisasi Situ Rawa Besar Sebagai Daya Tarik Wisata Air Di Kota Depok, Rahmi Setiawati Dec 2019

Program Revitalisasi Situ Rawa Besar Sebagai Daya Tarik Wisata Air Di Kota Depok, Rahmi Setiawati

Jurnal Sosial Humaniora Terapan

No abstract provided.

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Analysing Manufacturing Enterprises To Identify Opportunities For Automation And Guide Implementation - A Review., J Walker, S Childe, Y Wang Dec 2019

Analysing Manufacturing Enterprises To Identify Opportunities For Automation And Guide Implementation - A Review., J Walker, S Childe, Y Wang

Plymouth Business School

No abstract provided.

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Efficacy Of Ginger And Nutritox® In Counteracting Aflatoxin Effects On White Pekin Ducklings, Walaa Abu El-Ela, Kamel Abou.Elazm, Sanaa Awad Dec 2019

Efficacy Of Ginger And Nutritox® In Counteracting Aflatoxin Effects On White Pekin Ducklings, Walaa Abu El-Ela, Kamel Abou.Elazm, Sanaa Awad

Mansoura Veterinary Medical Journal

Objective: To evaluate the anti-aflatoxin effects of Ginger and Nutritox® in white Pekin ducklings. Design: Randomized experimental study Animals: Ninty- one-day-old white Pekin ducklings. Procedures: Ducklings were alienated randomly into six equal groups, each group in 3 replicates (each replicate, n=5). Control group G1) was fed on commercial broiler basal diet without any medications, while other experimental groups, namely 2, 3, 4, 5 and 6 were received diet containing: G2) basal diet (BD) + 300 ppb AF (aflatoxin); G3) BD + 300 ppb AF + 250 mg kg-1 Gr (Ginger); G4) BD +300 ppb AF +1gm/kg feed Nutritox®; G5) BD …

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The Role Of Paternal History Of Type 2 Diabetes Mellitus On Prediabetes Status Among The Offspring Of Type 2 Diabetes Mellitus Patients, Maulidia Ekaputri, Henrico Citrawijaya, Adrian Reynaldo Sudirman, Kevin Jonathan, Radityo Alu Murti, Ayu Putri Balqis, Dyah Purnamasari Dec 2019

The Role Of Paternal History Of Type 2 Diabetes Mellitus On Prediabetes Status Among The Offspring Of Type 2 Diabetes Mellitus Patients, Maulidia Ekaputri, Henrico Citrawijaya, Adrian Reynaldo Sudirman, Kevin Jonathan, Radityo Alu Murti, Ayu Putri Balqis, Dyah Purnamasari

Jurnal Penyakit Dalam Indonesia

No abstract provided.

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The Use Of Functional Traits To Identify Grasses And Fodder Shrubs For Domestication To Suit A Changing Climate, Meredith L. Mitchell, R. D. B. Whalley, Hayley C. Norman Dec 2019

The Use Of Functional Traits To Identify Grasses And Fodder Shrubs For Domestication To Suit A Changing Climate, Meredith L. Mitchell, R. D. B. Whalley, Hayley C. Norman

IGC Proceedings (1977-2023)

There is uncertainty about future climatic predictions; however there is little doubt amongst experts that the future will be warmer. Climate change and the associated elevation in atmospheric CO2 level and temperatures will provide novel challenges and potential opportunities for cultivated plant species. Plant breeding and domestication can contributed to improvements in both yield and quality of grasses and fodder shrubs. A range of key functional traits is required to cope with this changing climate. The main challenges that are discussed are new pests and pathogens; changes in the pattern of nutrient supply and forage quality; challenge associated with …

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Development Of New, High Yielding Tropical Grass Varieties For Increasing Productivity Of Semi-Arid Grasslands In India, Ajoy K. Roy, D. R. Malaviya, P. Kaushal, Sunil Kumar, S. Ahmed Dec 2019

Development Of New, High Yielding Tropical Grass Varieties For Increasing Productivity Of Semi-Arid Grasslands In India, Ajoy K. Roy, D. R. Malaviya, P. Kaushal, Sunil Kumar, S. Ahmed

IGC Proceedings (1977-2023)

Indian agriculture has traditionally been a mixed farming system since ancient times with integration of arable crops and livestock. It provides employment and livelihood to 70% of the rural population. Livestock are predominantly cattle and small ruminants that graze extensively on rangelands/pasturelands and common property resources (CPRs). CPRs occupy approximately 54 million ha area including permanent pastures in the alpine and temperate parts as well as seasonal grazing lands and wastelands. The productivity and carrying capacity of tropical degraded grasslands is very low, the average carrying capacity being 1.0, 0.7, 0.7 Adult Cattle Unit (ACU)/ha in semi-arid, arid and hill …

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Genetic Diversity And Relationships In Cocksfoot By Molecular Markers, Bing Zeng, Fu-Yuan Zuo, Xinquan Zhang, Liang Huan Dec 2019

Genetic Diversity And Relationships In Cocksfoot By Molecular Markers, Bing Zeng, Fu-Yuan Zuo, Xinquan Zhang, Liang Huan

IGC Proceedings (1977-2023)

Cocksfoot (Dactylis glomerata L.) originated from the Northern Hemisphere, including Eurasia and North Africa. It is widely distributed in temperate climates and has been widely used in the world more than 100 years, especially in North America, Europe and Japan. Genetic variability of cocksfoot had been detected by RAPD (Kölliker et al. 1999), and the correlation between genome size of natural populations of cocksfoot and location altitude has been analyzed by AFLP (Reeves et al. 1999). To date, information on the genetic diversity of cocksfoot at the molecular level is still scarce, This study is the first to …

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Methods Of Ryegrass Establishment (Lolium Multiflorum Lam.) Affecting Optimal Sward Height To Maximize The Intake Rate, Delma Fabíola Ferreira Da Silva, Lidiane Fonseca, Carolina Bremm, Anibal De Moraes, Alda L. G. Monteiro, Hélio Mayer, Thiago Marzarotto, Paulo C. De F. Carvalho Dec 2019

Methods Of Ryegrass Establishment (Lolium Multiflorum Lam.) Affecting Optimal Sward Height To Maximize The Intake Rate, Delma Fabíola Ferreira Da Silva, Lidiane Fonseca, Carolina Bremm, Anibal De Moraes, Alda L. G. Monteiro, Hélio Mayer, Thiago Marzarotto, Paulo C. De F. Carvalho

IGC Proceedings (1977-2023)

In integrated systems it is common to use the no-tillage method. The adoption of this method improves the system's sustainability (Laurent et al. 2011). It is necessary to understand the effects of the integrated systems on sward structure and its consequences in the grazing process and in animal production. The intake rate of grazing animals is primarily responsible for the animal performance (Coleman 2006), which short-termdepends mainly on sward structure (Laca and Demment 2006). The sward height has great influence on the animal decision on where to take the next bite (Mcgilloway et al. 1999). The hypothesis of …

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Timely Autumn Seeding Of Annual Ryegrass Is Essential For High Yield, Jennifer M. Johnson, Edzard Van Santen Dec 2019

Timely Autumn Seeding Of Annual Ryegrass Is Essential For High Yield, Jennifer M. Johnson, Edzard Van Santen

IGC Proceedings (1977-2023)

The use of annual ryegrass (Lolium multiflorum Lam.) as a winter cover crop and grazing option in the Southeast Unit-ed States has become a common practice. Recent research evaluating the effects of seeding time on seed yield in Canada determined autumn seeding produces the most desired results relative spring seeding, but indicated that varied autumn seeding rates would further their findings (Coulman et al. 2013). A University of Arkansas study utilized cool season annuals, wheat (Triticum aestivum L.) and annual ryegrass, to evaluate animal performance and seeding date effects. This research indicated that seeding cool-season annuals in …

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Global Illumina Sequencing And The Development Of Est-Ssr Markers In Alfalfa, Zhipeng Liu, Yanrong Wang Dec 2019

Global Illumina Sequencing And The Development Of Est-Ssr Markers In Alfalfa, Zhipeng Liu, Yanrong Wang

IGC Proceedings (1977-2023)

RNA-Seq, a massively parallel sequencing method for transcriptome analysis, only analyzes transcribed portions of the genome. Recently, RNA-Seq has provided an opportunity to expand the identification of alfalfa (Medicago sativa) genes. Using Illumina sequencing, 124,025 unique sequences from MSGI 1.0 have been identified from the elongating stem and post-elongation stem internodes of two alfalfa genotypes (Yang et al. 2011). Using 454 sequencing, 54,216 unique sequences were obtained from the roots and shoots of two alfalfa genotypes (Han et al., 2011). In addition, Illumina sequencing of old and young stems of 27 alfalfa genotypes led to the …

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