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Full-Text Articles in Entire DC Network
Estimating Leaf Area Index And Aboveground Biomass Of Grazing Pastures Using Sentinel-1, Sentinel-2 And Landsat Images, Jie Wang, Xiangming Xiao, Rajen Bajgain, Patrick Starks, Jean Steiner, Russell B. Doughty, Qing Chang
Estimating Leaf Area Index And Aboveground Biomass Of Grazing Pastures Using Sentinel-1, Sentinel-2 And Landsat Images, Jie Wang, Xiangming Xiao, Rajen Bajgain, Patrick Starks, Jean Steiner, Russell B. Doughty, Qing Chang
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Grassland degradation has accelerated in recent decades in response to increased climate variability and human activity. Rangeland and grassland conditions directly affect forage quality, livestock production, and regional grassland resources. In this study, we examined the potential of integrating synthetic aperture radar (SAR, Sentinel-1) and optical remote sensing (Landsat-8 and Sentinel-2) data to monitor the conditions of a native pasture and an introduced pasture in Oklahoma, USA. Leaf area index (LAI) and aboveground biomass (AGB) were used as indicators of pasture conditions under varying climate and human activities. We estimated the seasonal dynamics of LAI and AGB using Sentinel-1 (S1), …
Effects Of Childhood Trauma On Neurological Development And Mental, Physical Health In Adulthood, Sara Mckissick
Effects Of Childhood Trauma On Neurological Development And Mental, Physical Health In Adulthood, Sara Mckissick
D.U.Quark
Unfortunately, childhood maltreatment and trauma are prevalent in society. As awareness grows, so does the research on the long-term effects early exposure to traumatic events has on the developing person. Socioeconomic hardships, psychopathologies, and cognitive deficiencies correlate with early childhood adverse experiences. This review will compare normal neurological activity and development to that of children who have experienced childhood trauma. It will also further explore various factors such as the level of trauma, the onset of exposure, duration or recurrence of trauma experienced. Finally, this review will examine the effects that manifest themselves in adulthood to further understand the detrimental …
Buzz Kill, Robbin Ray
Revision Of The Genus Gilia Of Utah, Zachariah Jaramillo, Dr. Leigh Johnson
Revision Of The Genus Gilia Of Utah, Zachariah Jaramillo, Dr. Leigh Johnson
Journal of Undergraduate Research
The genus Gilia has historically been difficult to work with because identification of the genus via a dichotomous key relies heavily on the absence of characteristics instead of the presence of unique characteristics (Johnson et al., 2004). This has caused Gilia to become a polyphyletic group that serves as a “dumping ground” for odd species that do not easily fit in any other genus within the family. Studies using morphological and molecular data have identified species traditionally placed in Gilia that are really more closely related to other genera. For example, Gilia gilioides belongs in Allophyllum, Gilia capillaris belongs in …
Perception Of Speech And Song In Religious Music: A Neurological Approach, Dagan Pielstick, Francesca Lawson
Perception Of Speech And Song In Religious Music: A Neurological Approach, Dagan Pielstick, Francesca Lawson
Journal of Undergraduate Research
The relationship between speech and song has been an area of interest in evolutionary biology and neuroscience over the past two decades. Some evolutionary biologists have hypothesized that music and language descended from a protolanguage in early human communication (Brown 2000). At the same time, developments in technology have made it possible to analyze the activation of different parts of the brain, allowing us to see traces of this evolutionary history in the neural pathways of the brain (Patel 2008). Recent studies on language-music relationships both support and contradict the theory of a common protolanguage base for music and language …
Damselfies Of Vanuatu, Madison Lallatin, Seth Bybee
Damselfies Of Vanuatu, Madison Lallatin, Seth Bybee
Journal of Undergraduate Research
Vanuatu is a string of active volcanic islands in Micronesia near Fiji and Australia. The islands undergo regular submersion and emersion from the ocean, and the most recent emergence was 2 million years ago (Hamilton et al. 2010). As a result, all organisms on the islands of Vanuatu have migrated there, likely from other islands, within the past 2 million years. Fiji is one likely candidate for contributing species, being geographically close to Vanuatu and exhibiting similar species. Fourteen of us went to Vanuatu for 6 weeks on a study abroad led by Dr. Seth Bybee to collect and observe …
Quantifying Predictive Value Of Biological Data Types In Machine Learning Models Of Cancer Outcome, Samantha Jensen, Stephen Piccolo
Quantifying Predictive Value Of Biological Data Types In Machine Learning Models Of Cancer Outcome, Samantha Jensen, Stephen Piccolo
Journal of Undergraduate Research
Precision medicine is a growing movement toward utilizing molecular diagnostics to guide medical decisions. It is particularly useful when applied to cancer treatment, as knowing details about cancer stage, genetic pathology, and tumor type can inform life-saving decisions. Increasingly, physicians may use genetic, proteomic, epigenetic, and expression data to determine treatment strategy and even choose specific chemotherapy drugs1.
Using Remote Video Systems To Measure Effectiveness Of Small Scale Mpas In Saipipi, Samoa, Calvin Quigley, Dr. Richard Gill
Using Remote Video Systems To Measure Effectiveness Of Small Scale Mpas In Saipipi, Samoa, Calvin Quigley, Dr. Richard Gill
Journal of Undergraduate Research
Coral reefs represent an invaluable natural, providing $30 billion dollars of fishing and recreational profits worldwide, and housing nearly a quarter of the oceans species [1, 2]. However, threats such as climate change, ocean acidification, coastal development and overharvesting have destroyed more than a fifth of the worlds coral reefs in recent decades[3]. One of the most common management plans that has been implemented to help protect and restore coral reefs is the establishment of marine protected areas (MPAs)[4]. MPAs have been proven to be effective in helping restore fish populations and coral cover, however the majority of MPAs are …
Effectiveness Of Hospital Disinfectants Against Clostridium Spores, Bejamin Ogilvie, Richard Robinson
Effectiveness Of Hospital Disinfectants Against Clostridium Spores, Bejamin Ogilvie, Richard Robinson
Journal of Undergraduate Research
Clostridium is a genus of anaerobic spore-forming bacteria notable for causing human infections. C. difficile has an especially high impact, affecting roughly 453,000 individuals in the U.S. in 2011 (1). C. tetani and C. perfringens infections occur much less frequently, although they remain clinically important. Disinfecting objects contaminated with Clostridium is challenging, as Clostridium spores are resistant to extreme temperature, desiccation, and most chemicals (2, 3). Clostridium is also frequently found within soil, making it a likely contaminant on almost any piece of medical equipment (3).
Currently, the U.S. EPA only requires sporicidal disinfectant efficacy tests against C. sporogenes, a …
Could Tk1 Suppress The Immune System And Promote Tumor Development?, Joshua Keller, Roman Kovtun, Kim O'Neill
Could Tk1 Suppress The Immune System And Promote Tumor Development?, Joshua Keller, Roman Kovtun, Kim O'Neill
Journal of Undergraduate Research
Cancer is a widespread disease that affects millions of people worldwide and in many cases is difficult to treat. In order to find new and better treatment, it is important to understand how this disease progresses. Thymidine Kinase 1 (TK1) is a recognized biomarker that is upregulated in cancer cells compared to healthy tissue. Although this protein is normally found inside the cells (cytosol), it becomes upregulated on the surface of malignant cells. Moreover, recent research shows that TK1 expression is further upregulated in breast cancer tissues that undergo a transition from stationary (epithelial) cells to more mobile (mesenchymal) cells. …
Regulation Of Trophoblast Invasion By Pyruvate Kinase Isozyme M2 (Pkm2): Preemptive To Pkm2 Activation Decreases Iugr Symptoms In Mice Exposed To Second-Hand Smoke, Juan Mejia, Juan Arroyo, Ph.D.
Regulation Of Trophoblast Invasion By Pyruvate Kinase Isozyme M2 (Pkm2): Preemptive To Pkm2 Activation Decreases Iugr Symptoms In Mice Exposed To Second-Hand Smoke, Juan Mejia, Juan Arroyo, Ph.D.
Journal of Undergraduate Research
The placenta is an organ composed of highly vascular tissue. It plays an essential role in healthy fetal development as a mediator of gases and nutrients between the mother and fetus. Many complications can occur in this stage of fetal development, such as intrauterine growth restriction (IUGR) when the placenta’s invasive and vascular roles are inhibited. IUGR is an obstetric complication that affects over 10% of the population causing harmful symptoms to the fetus and mother. Physiologically, in the first trimester, high trophoblast cell invasion occurs, and the spiral arteries dilate to create a low resistance, high capacity vessel capable …
Nrf2 In Protecting Against Valproic Acid-¬‐Induced Oxidative Injury During Cellular Differentiation, Alyssa Palmer, Jason Hansen
Nrf2 In Protecting Against Valproic Acid-¬‐Induced Oxidative Injury During Cellular Differentiation, Alyssa Palmer, Jason Hansen
Journal of Undergraduate Research
The causes of the majority of birth defects remain largely unknown today. However, many chemicals known to cause defects are also known inducers of oxidative stress, resulting in faulty cellular function. This suggests an important link between oxidative stress and normal and abnormal embryonic development [1]. Oxidative stress is a phenomenon caused by an imbalance in the production of reactive molecules derived from oxygen called reactive oxygen species (ROS). Although basal levels of ROS are necessary in order for normal cell function to occur, an overabundance of ROS interact with molecules that they shouldn’t, disrupting vital cellular pathways. This is …
Board Of Trustees Agenda, Jun. 21 2019, Indiana State University Board Of Trustees
Board Of Trustees Agenda, Jun. 21 2019, Indiana State University Board Of Trustees
Board of Trustees
No abstract provided.
Towards On-Demand E. Coli-Based Cell-Free Protein Synthesis Of Tissue Plasminogen Activator, Seung-Ook Yang, Gregory H. Nielsen, Kristen M. Wilding, Merideth A. Cooper, David W. Wood, Bradley Charles Bundy
Towards On-Demand E. Coli-Based Cell-Free Protein Synthesis Of Tissue Plasminogen Activator, Seung-Ook Yang, Gregory H. Nielsen, Kristen M. Wilding, Merideth A. Cooper, David W. Wood, Bradley Charles Bundy
Faculty Publications
Stroke is the leading cause of death with over 5 million deaths worldwide each year. About 80% of strokes are ischemic strokes caused by blood clots. Tissue plasminogen activator (tPa) is the only FDA-approved drug to treat ischemic stroke with a wholesale price over $6000. tPa is now off patent although no biosimilar has been developed. The production of tPa is complicated by the 17 disulfide bonds that exist in correctly folded tPA. Here, we present an Escherichia coli-based cell-free protein synthesis platform for tPa expression and report conditions which resulted in the production of active tPa. While the activity …
Submittal Of Mixed Waste Landfill Annual Long-Term Monitoring & Maintenance Report, April 2018-March 2019 For Sandia National Laboratories/New Mexico, Epa Id Number Nm5890110518, June 2019, Sandia National Laboratories/Nm Snl
Submittal Of Mixed Waste Landfill Annual Long-Term Monitoring & Maintenance Report, April 2018-March 2019 For Sandia National Laboratories/New Mexico, Epa Id Number Nm5890110518, June 2019, Sandia National Laboratories/Nm Snl
Facilities-Units
The Department of Energy/National Nuclear Security Administration (DOE/NNSA) and Sandia National Laboratories /New Mexico (SNL/NM) personnel submitted the Mixed Waste Landfill (MWL) Annual Long-Term Monitoring and Maintenance Report for the April 2018 through March 2019 reporting period to the New Mexico Environment Department (NMED) in accordance with the requirements of Section 4.8.1 of the MWL Long-Term Monitoring and Maintenance Plan (LTMMP). The LTMMP became effective on January 8, 2014.
The MWL is a 2.6-acre Solid Waste Management Unit (SWMU 76) located in the north-central portion of SNL/NM Technical Area III that has undergone corrective action. As of March 13, 2016, …
Typha (Cattail) Invasion In North American Wetlands: Biology, Regional Problems, Impacts, Ecosystem Services, And Management, Sheel Bansal, Shane C. Lishawa, Sue Newman, Brian A. Tangen, Douglas Wilcox, Dennis Albert, Michael J. Anteau, Michael J. Chimney, Ryann L. Cressey, Edward Dekeyser, Kenneth J. Elgersma, Sarah A. Finkelstein, Joanna Freeland, Richard Grosshans, Page E. Klug, Daniel J. Larkin, Beth A. Lawrence, George Linz, Joy Marburger, Gregory Noe, Clint Otto, Nicholas Reo, Jennifer Richards, Curtis Richardson, Leroy Rodgers, Amy J. Schrank, Dan Svedarsky, Steven Travis, Nancy Tuchman, Lisamarie Windham-Myers
Typha (Cattail) Invasion In North American Wetlands: Biology, Regional Problems, Impacts, Ecosystem Services, And Management, Sheel Bansal, Shane C. Lishawa, Sue Newman, Brian A. Tangen, Douglas Wilcox, Dennis Albert, Michael J. Anteau, Michael J. Chimney, Ryann L. Cressey, Edward Dekeyser, Kenneth J. Elgersma, Sarah A. Finkelstein, Joanna Freeland, Richard Grosshans, Page E. Klug, Daniel J. Larkin, Beth A. Lawrence, George Linz, Joy Marburger, Gregory Noe, Clint Otto, Nicholas Reo, Jennifer Richards, Curtis Richardson, Leroy Rodgers, Amy J. Schrank, Dan Svedarsky, Steven Travis, Nancy Tuchman, Lisamarie Windham-Myers
United States Department of Agriculture Wildlife Services: Staff Publications
Typha is an iconic wetland plant found worldwide. Hybridization and anthropogenic disturbances have resulted in large increases in Typha abundance in wetland ecosystems throughout North America at a cost to native floral and faunal biodiversity. As demonstrated by three regional case studies, Typha is capable of rapidly colonizing habitats and forming monodominant vegetation stands due to traits such as robust size, rapid growth rate, and rhizomatic expansion. Increased nutrient inputs into wetlands and altered hydrologic regimes are among the principal anthropogenic drivers of Typha invasion. Typha is associated with a wide range of negative ecological impacts to wetland and agricultural …
Factors Considered By Special Education Case Managers When Making A Decision To Mainstream Emotionally Disturbed Students From A Self-Contained Classroom To General Education Classes, Pamela Butler-Harris
Factors Considered By Special Education Case Managers When Making A Decision To Mainstream Emotionally Disturbed Students From A Self-Contained Classroom To General Education Classes, Pamela Butler-Harris
Dissertations
Purpose: This qualitative comparative case study analyzes the perspectives, feelings, and beliefs of eight special education case managers who work with students who have been diagnosed as emotionally disturbed (ED), or exhibit ED type behaviors in Contra Costa County, California. It is the hope that through the identification of criteria leading to successful integration, policy makers and educators will be able to develop and implement effective strategies to successfully mainstream ED students, or students who exhibit ED type behaviors into general education classes.
Methodology: This qualitative comparative case study is designed around the following research question: What factors do special …
History And Main Components Of Content And Language Integrated Learning Approach (Clil), Munis Khusanova Techear Of Linguistics And Literary Studies Department
History And Main Components Of Content And Language Integrated Learning Approach (Clil), Munis Khusanova Techear Of Linguistics And Literary Studies Department
Philology Matters
The article is devoted to analysis of the development of CLIL approach which is gaining considerable attention of world educators because ofits dual aim: teaching a foreign language through content and teaching content through a foreign language. The material of the study was a theoretical framework offered by the founders of this approach. Objective: to identify the role of the main components of this approach called “4C”s– content, communication, cognition and culturewhich should be dwelled into every CLIL class-room. Along with it, it presents the principles and ways of integrating CLIL into the school’s educational program. The author focuses on …
Autobiography As A Genre Feature Of Coming-Of-Age Novels By Ch. Dickens, Nargiza Makhmudova Senior Teacher
Autobiography As A Genre Feature Of Coming-Of-Age Novels By Ch. Dickens, Nargiza Makhmudova Senior Teacher
Philology Matters
The article explores autobiography as one of the genre features of the coming-of-age novels in the context of Charles Dickens’ legacy. Dickens’ realistic novels appeared as a result of his own life experience; the writer wanted to bring and share his views, ideas, life lessons with the readers and teach them to what he considered the correct moral behavior. Episodes of the author’s life, as if scattered through all his novels about a young man such as “Nicholas Nickleby”, “Martin Chuzzlewit”, “David Copperfield” and “The Great Expectations”. Autobiographical genre features are revealed in the coming-of-age novels in two ways– through …
The Results Of The Corpus Analysis As A Quantitative Representation Of Linguocultural Concepts, Nozimjon Ataboyev Phd Student
The Results Of The Corpus Analysis As A Quantitative Representation Of Linguocultural Concepts, Nozimjon Ataboyev Phd Student
Philology Matters
The article deals with the relationship between the newly emerged and fast developing corpus linguistics as a research methodology and the sphere of linguoculturology. The language corpora are regarded as the source of examples representing cultural notions in the process of creating conceptual frames. In particular, the results of corpus analysis have been based on the modern English corpora as “COCA” and “BNC”. The examples related to the application of the notion of “family” have been analyzed by means of several searches through the concordance on the corpus platform. In order to make the final conclusions, the gathered results have …
Pathogenetic Reasons For The Development Of Varicose Disease In Pregnant Women, N.K. Dustova, G.S. Babadjanova, G.A. Ikhtiyarova
Pathogenetic Reasons For The Development Of Varicose Disease In Pregnant Women, N.K. Dustova, G.S. Babadjanova, G.A. Ikhtiyarova
Central Asian Journal of Pediatrics
Purpose of research. To identify the causes, to study the effectiveness of a differentiated approach to the management of pregnancy and childbirth in women with varicose veins. Material and methods. We have prospectively examined in terms 10-41 weeks of pregnancy, childbirth and the early postpartum period of 120 patients with varicose disease (VD), All surveyed pregnant women were divided into 3 groups: I group – the study group consisted of 75 pregnant women with varicose veins of the lower extremities (VVTLE).Comparison group II consisted of 45 patients with pelvic varicose veins (PVV).The control group consisted of 30 patients with uncomplicated …
Results Of Biologic Marking Of Primary Glaucoma In Uzbek Population In Conditions Of Panmixia, B.T. Buzrukov, L.D. Babadjanova, D.B. Buzrukova
Results Of Biologic Marking Of Primary Glaucoma In Uzbek Population In Conditions Of Panmixia, B.T. Buzrukov, L.D. Babadjanova, D.B. Buzrukova
Central Asian Journal of Pediatrics
An aim of research was to establish a type of heredity of primary glaucoma in both panmix and inbred population of patients. An analysis of 66 parents (33 families) served material of research. A method of Weinberg was used for an analysis of data. In conditions of inbreeding 18 families and 5 types of inbred marriages were studied and analyzed. As result of testing primary glaucoma (PG) in conditions of panmixia and inbreeding a lack of correspondence to any classic Mendel’s model has been found. Data obtained in borders of multifactor theory coincided with this type of heredity. All that …
Features It Is Judicial-Medical Examinations Of Cranial Brain Trauma At Children, A.A. Beloborodov, A.I. Iskandarov
Features It Is Judicial-Medical Examinations Of Cranial Brain Trauma At Children, A.A. Beloborodov, A.I. Iskandarov
Central Asian Journal of Pediatrics
According to the spent researches at children the clinical semiology was characterised by the raised reactance of an organism on a trauma, generalisation of processes of excitation and braking, rough vegetative reactions, bent to hyperergic reactions. At performance of a forensic medical examination comparison of terms of frustration of health to weight of damage at children duration of treatment is used as one of criteria at qualification of damages.
The Effect Of Immunomoduline And Phytin Compounds On Lipid Peroxidation Induced At Toxic Experimental Hepatitis, K.T. Mirzaahmedova
The Effect Of Immunomoduline And Phytin Compounds On Lipid Peroxidation Induced At Toxic Experimental Hepatitis, K.T. Mirzaahmedova
Central Asian Journal of Pediatrics
The influence of immunomodulin, phytin compounds as phytin-C and cobalt phytate and their combinations on lipid peroxidation processes in experimental toxic hepatitis in rats was studied. It was found that the studied drugs in carbon tetrachloride poisoning had a unidirectional antioxidant effect. Combined administration of immunomodulin with phytin compounds increased antioxidant activity.
Hygienic Aspects Of Labor Protection In Livestock Production Complexes And Milk Production Farms, Kh.O. Kasimov
Hygienic Aspects Of Labor Protection In Livestock Production Complexes And Milk Production Farms, Kh.O. Kasimov
Central Asian Journal of Pediatrics
The cattle-breeding complexes and farms workers are exposed to adverse climate, chemical and biological factors throughout the year. The presence of ammonia, hydrogen sulfide, dust and high bacterial contamination in the air takes on a special role. All of the above dictates the need to develop optimal working conditions and protect the health of workers in livestock farms and complexes.
Integrated Assessment Of Risk Factors Of Post-Infarction Depression, Nigina Mukhamadieva, Zebiniso Tuksanova
Integrated Assessment Of Risk Factors Of Post-Infarction Depression, Nigina Mukhamadieva, Zebiniso Tuksanova
Central Asian Journal of Pediatrics
Depressive disorders are among the most common types of mental pathology. According to various epidemiological data, depression affects 4 to 10% of the world's population. The aim of this study was an integrated assessment of risk factors for depressive disorders and the development of an algorithm to assess the risk of post-infarction depression. The object of the study was patients with myocardial infarction (MI) hospitalized in a hospital (754 patients). We conducted a study in the first week after MI in the presence of depressive symptoms in patients, it is necessary to consult a psychiatrist for the clinical diagnosis of …
Praemonitus, Praemunitus: Assessing The Ethical Dimension Of The Globalisation Of Science And Technology, F. F. Sattarov
Praemonitus, Praemunitus: Assessing The Ethical Dimension Of The Globalisation Of Science And Technology, F. F. Sattarov
International Relations: Politics, Economics, Law
In recent years, there has been a growing awareness of ethical issues resulting from the globalisation of science and technology. This article identifies and reviews the most important of such ethical issues in a systematic fashion. This paper aims to show that an increase in science research and technology development with foreign partners can have its own ethical and moral perils. Hence, to prepare the country and society to avoid the negative effects and consequences of the scientific and technological globalisation, it is necessary first to have a clear picture of all possible ethical issues, particularly those which either remain …
Stephen Liben Oral History.
Pediatric Palliative Care Oral History Project
Dr. Liben credits his initial venture into pediatric palliative care to his experiences of witnessing "unnecessary suffering" during his career in pediatric critical care. Dr. Liben states that he was attracted to how much "promise" that pediatric palliative care held in addressing more than just the medical needs of children and their families. He then recalls experiences he had with international leaders in the emerging field of pediatric palliative care. Dr. Liben describes how the field has evolved to be more patient- and family-centric while spanning across the intersections of pain management, chronic care, and complex care. Dr. Liben also …
Cd5 Deficient Mice Exhibit Altered Cognitive Function In Behavioral Studies, Tyler Cox, Dr. Scott Weber
Cd5 Deficient Mice Exhibit Altered Cognitive Function In Behavioral Studies, Tyler Cox, Dr. Scott Weber
Journal of Undergraduate Research
The immune system plays a role in protecting and maintaining the central nervous system (CNS). 1Dysfunctions within the immune system can affect the CNS and it is associated with diseases such as Multiple Sclerosis, Alzheimer’s and Parkinson’s disease. Having an imbalanced activation of immune cells within CNS results in cognitive impairments. CD5 is a protein on the surface of T cells and certain B cells that inhibits cellular activation and prevents immune cells from reacting too strongly to antigens and causing autoimmunity to our own tissues. Modulating CD5 and the immune system could affect the CNS and cognitive function. Observations …
Characterization Of Mutant Chemokine (C-C) Motif Receptor-Like 2 (Ccrl2) And Its Role In Inflammation And Alzheimer’S Disease, Meganne Ferrel, Dr. John S. K. Kauwe
Characterization Of Mutant Chemokine (C-C) Motif Receptor-Like 2 (Ccrl2) And Its Role In Inflammation And Alzheimer’S Disease, Meganne Ferrel, Dr. John S. K. Kauwe
Journal of Undergraduate Research
Alzheimer’s Disease (AD) is identified as a proteopathic disease that results from an extensive accumulation of amyloid plaques and neurofibrillary tangles in the brain. Several researchers have discovered that cerebrospinal fluid (CSF) contains biomarkers for AD such as Amyloid-beta and tau. Neuritic plaques in AD are surrounded by activated microglia and astrocytes which can initiate complement and inflammation in the presence of amyloid-beta. Macrophage inflammatory protein chemokine (C-C motif) ligand 4 (CCL4) is a biomarker found in CSF. A high level of expression is associated with risk of developing AD. CCL4 is expressed in a subpopulation of reactive astrocytes and …