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Evaluation Of Adam33 Gene’S Single Nucleotide Polymorphism Variants Against Asthma And The Unique Pattern Of Inheritance In Northern And Central Punjab, Pakistan, Muhammad U. Ghani, Muhammad F. Sabar, Iqbal Bano, Mariam Shahid, Muhammad Akram, Ifrah Khalid, Alishba Maryam, Muhammad U. Khan

Saudi Medical Journal

Objectives: To investigate the relationship of 3 single nucleotide polymorphism (SNP) variants of ADAM33 with asthma susceptibility in patients from Northern and Central Punjab, Punjab, Pakistan. Methods: In this case-control study, healthy and asthmatic participants were recruited between 2015 and 2017. The SNPs of ADAM33 gene, rs2280089, rs2280090, and rs2280091 were analyzed in 296 asthma patients and 343 healthy controls, as well as linkage disequilibrium and haplotype analysis. Results: The non-significant differences were observed in allele and genotype frequencies of the SNPs in asthmatic and healthy persons even after population stratification based on age, caste, gender, family history, and environment. …

Amyloidogenicity Of Naturally Occurring Full-Length Animal Iapp Variants, Larry M. Palato, Shannon Pilcher, Alissa Oakes, Arleen Lamba, Jaris Torres, Litza I. Ledesma Monjaraz, Crystabel Munoz, Edward Njoo, Dillon J. Rinauro, Kate Alexandra Menefee, Angela Tun, Betssy L. Jauregui, Sarah Shapiro, Olivia H. Nossiff, Eileen Olivares, Kevin Chang, Viviane Nguyen, Luiza A. Nogaj, David A. Moffet

Chemistry and Biochemistry Faculty Works

Aim: To identify naturally occurring variants of IAPP capable of inhibiting the aggregation of human IAPP and protecting living cells from the toxic effects of human IAPP. Background: The loss of insulin-producing β-cells and the overall progression of type 2 diabetes appears to be linked to the formation of toxic human IAPP (hIAPP, Islet Amyloid Polypeptide, amylin) amyloid in the pancreas. Inhibiting the initial aggregation of hIAPP has the potential to slow, if not stop entirely, the loss of β-cells and halt the progression of the disease. Objective: To identify and characterize naturally occurring variants of IAPP capable of inhibiting …

Going Beneath The Surface: The Ecology Of Metalimnetic Cyanobacteria Layers In Three New Hampshire Lakes, Sabina Perkins

Master's Theses and Capstones

Cyanobacteria blooms take different forms: surface scums, subsurface epilimnetic blooms, benthic mats, and in some lakes, metalimnetic layers. There is limited field research on the seasonal behavior of metalimnetic layers and the contribution of layer-produced cyanotoxins to the overall toxin profile of New Hampshire lakes. Three lakes in New Hampshire were monitored monthly pre-summer stratification through post-fall mixis in 2018, tracking the formation and persistence of deep-water cyanobacteria layers and the physical/chemical/light environments where they were found. Cyanobacteria abundance and dominance were quantified through cell counts using an Imaging Flow Cytobot (IFCB) and with phycocyanin fluorescence estimates. Persistent, nearly monospecific …

The Effects Of Climate Change On The Heart Rates & Growth Of Sea Slugs In The Gulf Of Maine, Jennifer Leigh Gibson

Master's Theses and Capstones

In the next 80 years, sea surface temperatures are expected to increase by 1.5o to 2oC and ocean pH is expected to drop by 0.06 to 0.32 units, with exacerbated effects seen in coastal waters. Temperature increase has already forced organisms to shift their range polewards and ocean acidification has negatively affected calcifying organisms. Interactive effects, only more recently studied, vary depending on phylum and life cycle stage. This study examined both the upper thermal tolerance and interactive effect of temperature and acidification on the heart rate of five cold-water species of nudibranchs (Aeolidia papillosa, Cuthona gymnota, Dendronotus frondosus, Flabellina …

The Development Of A Biometric Keystroke Authentication Framework To Enhance System Security, Hector Suarez

Masters Theses and Doctoral Dissertations

Computer systems have proven to be essential to achieving our daily tasks such as managing our banking accounts, managing our health information and managing critical information systems such as drinking water systems or nuclear power plant systems. Such distributed systems are networked and must be protected against cyber threats. This research presents the design and implementation of a stand alone web based biometric keystroke authentication framework that creates a user's keystroke typing profile and use it as a second form of authentication. Several biometric models were then bench marked for their accuracy by computing their EER. By using keystroke biometrics …

Experimentation And Data Collection Involving Allee Effects And Virulence Determinations Of Vibrio Cholerae, Erick Rojas

Masters Theses and Doctoral Dissertations

The proposed research focuses on the growth and potential risk associated with virulent spread of Vibrio cholerae. The thesis project is based upon a mathematical and computational simulation of pathogen dynamics that is validated via experimentation and data collection focused on the transmission and pathogenic evolution of Vibrio cholerae. While the larger scope of the project is focused around the importance of understanding Vibrio cholerae dynamics to create control and management strategies that may prevent future outbreaks, this thesis focuses on the experimentation and subsequent data collection surrounding the growth mechanisms of the bacterium under various environmental conditions. In addition, …

The Effects Of Urbanization On Winter Avian Diversity: A Citizen Science And Gis Study, Caryn Ross

Masters Theses and Doctoral Dissertations

As the human population continuously increases, habitat transformation due to urban expansion is inevitable, affecting many wildlife species. Birds have become a frequently used model species for studying the effects of urbanization on wildlife populations due to their environmental sensitivity and ease of observation. While previous studies have investigated changes in species richness and abundance related to urbanization, there is a need for more broad-scale studies geographically associating changes in bird diversity and urban expansion. Through the use of extensive bird census data and high-resolution digitized land use data, this study revealed that species richness, diversity, and evenness within count …

Influential Women In The History Of American Music Education, Christina Genae Mathis

Masters Theses

Early American music education is rooted not only in the work of Lowell Mason and other male music education pioneers, but also in the lives of a number of women whose work in the field was largely unnoticed until the twentieth century and the evolution of women’s rights in America. This project researched the lives and contributions of five of these women: Frances Elliott Clark, one of the first educators to take advantage of the technology available at the time, who incorporated the use of phonographs in the music classroom; Julia Ettie Crane, whose legacy lives on through the Crane …

Molecular Responses To Deepwater Horizon Oil Spill Contaminants In Estuarine Fish, Elizabeth Jones

Dissertations

The 2010 Deepwater Horizon (DWH) oil spill resulted in the oiling of approximately 2100 km of shoreline. During that time, resident organisms had to contend with the effects of multiple contaminants and suboptimal environmental conditions. This research examines the molecular effects of DWH oil spill contaminants in estuarine fish (CyprinodonvariegatusandFundulusgrandis) across multiple life stages, contaminant concentrations, and in conjunction with environmental stressors.

Our results indicate that:

1. Oil elicits substantial transcriptional effects across life stages in C. variegatus. In adults, exposure to low concentrations of oil and dispersant results in transcriptional effects related …

Systemic Immunologic Consequences Of Chronic Periodontitis, D. K. Gaudilliere, A. Culos, K. Djebali, Amy S. Tsai, Edward A. Ganio, W. M. Choi, Xiaoyuan Han, A. Maghaireh, B. Choisy, Q. Baca, J. F. Einhaus, J. J. Hedou, B. Bertrand, K. Ando, R. Fallahzadeh, Mohammad Sajjad Ghaemi, R. Okada, N. Stanley, A. Tanada, M. Tingle, T. Alpagot, J. A. Helms, M. S. Angst, N. Aghaeepour, B. Gaudilliere

All Dugoni School of Dentistry Faculty Articles

Chronic periodontitis (ChP) is a prevalent inflammatory disease affecting 46% of the US population. ChP produces a profound local inflammatory response to dysbiotic oral microbiota that leads to destruction of alveolar bone and tooth loss. ChP is also associated with systemic illnesses, including cardiovascular diseases, malignancies, and adverse pregnancy outcomes. However, the mechanisms underlying these adverse health outcomes are poorly understood. In this prospective cohort study, we used a highly multiplex mass cytometry immunoassay to perform an in-depth analysis of the systemic consequences of ChP in patients before (n = 28) and after (n = 16) periodontal treatment. A high-dimensional …

Synthesis, Characterization And Biological Evaluation Of Novel (S,E)-11-[2-(Arylmethylene) Hydrazono] Pyrrolo [2,1-C] [1,4] Benzodiazepine Derivatives, David Mingle

Electronic Theses and Dissertations

Pyrrolo [2,1-c] [1,4] benzodiazepine (PBD) is a class of natural products obtained from various actinomycetes which have both anti-tumor and antibiotic activities and can bind to specific sequences of DNA. PBD-dilactam was initially produced using isatoic anhydride and (L)-proline which was then converted to the PBD-thiolactam using Lawesson's reagent. Reaction of thiolactam with hydrazine in ethanol afforded PBD-11-hydrazinyl. Condensation of 11-hydrazinyl PBD with aldehydes possessing various substitutions was performed to obtain (S,E)-11-[2-(arylmethylene) hydrazono] pyrrolo [2,1-c] [1,4] benzodiazepine derivatives. ¹HNMR, ¹³C-NMR, DEPT, IR, GC-MS and X-ray crystallography were used for the characterization. Inhibition activity of the products …

The Salivary Alpha-Amylase Response To Resistance Training, Asher Flynn

Electronic Theses and Dissertations

The purposes of this dissertation were to investigate the response of salivary alpha-amylase to a single resistance training session and to a week-long resistance training over-reaching protocol. The major findings of this dissertation are as follows:

Study 1 – A single resistance training session consisting of 5 sets of 10 repetitions of squat and bench press at 95 percent of repetition maximum creates a statistically significant increase in salivary alpha-amylase concentrations.

Study 2 – Two resistance training sessions consisting of 5 sets of 10 repetitions of squat and bench press at 95 percent of repetition maximum within 5 days does …

Assessing Heat-Related Mortality And Morbidity Risks In Rural Populations And Sub-Populations, Emmanuel A. Odame

Electronic Theses and Dissertations

Heat stress is an environmental and occupational hazard exacerbated by climate change. Rural populations and sub-populations continue to experience disproportionate risks of heat-related impacts due to their low adaptive capacities in terms of infrastructure, information and other resources which are critical in dealing with heat. The study goals were to determine heat-related mortality risks in rural populations globally, explore the contribution of the outdoor work environment and other factors in association with occupational heat-related illnesses (HRI), and assess the risk of heat stress among crop workers using the Wet Bulb Globe Temperature (WBGT). Published peer-reviewed scientific literature on heat-related mortality …

Empirical Bayes Estimators And Borel-Tanner Distribution, Celestina Ruby Soltero

Theses and Dissertations

The motivation for this paper stems from the role Borel-Tanner (BT) distribution has as the distribution of the total outbreak number in epidemics modeled by branching processes. We briefly review Borel-Tanner distribution and its applications. In Chapter II we outline the Bayes decision problem, a construction for an Empirical Bayes (EB) estimator proposed by Liang [9] and discuss risk analysis. In Chapter III, the importance of randomization addressed and a classical construction of a monotonized EB estimator proposed by Houwalingen [14] is outlined. Lastly in Chapter IV we use R software to perform a Monte Carlo simulation and conduct a …

Factors Influencing Farmers’ Adoption And Intentions To Adopt Pollinator Conservation Programs And Practices In Illinois, U.S.A., Christopher M. Sedivy

Theses

Due to the growing recognition of the social and ecological consequences of the global decline in pollinator species, the need for more effective policies for the conservation of pollinator habitat is now more than ever. These trends call for research that provides a deeper understanding of farmers' decision-making processes. In this regard, this study tested a modified version of the Theory of Planned Behavior as a conceptual model for explaining farmers' perceptions and behavior regarding the adoption of pollinator conservation programs and practices. Specifically, the study tested how farmers' perceived behavioral control, attitudes, subjective norms, concern about herbicide resistance issues, …

The Assessment Of Resting Tongue Posture In Different Sagittal Skeletal Patterns, Farheen Fatima, Mubassar Fida

Section of Dental-Oral Maxillofacial Surgery

INTRODUCTION:
Resting tongue posture affects the surrounding structures and, theoretically, may result in altered arch form and jaw relationship.
OBJECTIVE:
The objective of the present study was to investigate the association between resting tongue posture as observed in lateral cephalometric radiograph, sagittal jaw relationship and arch form.
METHODS:
The study was conducted on pretreatment lateral cephalograms and dental casts of 90 subjects. Subjects were equally divided into three groups, based on sagittal jaw relationship (Class I, II and III). Tongue posture was determined in terms of tongue-to-palate distances at six different points (distances 1 to 6) using ViewPro-X software, according …

Isolation And Culture Of Dental Pulp Stem Cells From Permanent And Deciduous Teeth, Shagufta Naz, Farhan Raza Khan, Raheela Rahat, Sahreena Ladhani, Mehwish Saghir Khan, Nuruddin Badruddin Mohammed, Tashfeen Ahmad

Section of Dental-Oral Maxillofacial Surgery

OBJECTIVE:
To isolate dental pulp mesenchymal stem cells (MSCs) from non-infected human permanent and deciduous teeth.
METHODS:
It was an in-vitro experimental study. Human teeth were collected from 13 apparently healthy subjects including nine adults and four children. After decoronation dental pulps were extirpated from teeth and cultured via explant method in a stem cell defined media. Data was analyzed by descriptive statistics. RESULTS:
As above MSCs emerged exhibiting fibroblast-like morphology. In vitro culture was positive for 100% (9/9) and 75% (3/4) of the permanent and deciduous teeth respectively. First cell appeared from deciduous teeth pulp in 10±6.2 days while …

Stem Heroes: A Narrative-Based Intervention To Increase Self-Efficacy And Interest In Science, Technology, Engineering, And Mathematics In Elementary School-Aged Children, Lauren M. Carino

Seton Hall University Dissertations and Theses (ETDs)

Over time, researchers have struggled to identify effective interventions to support girls’ self-efficacy and interest in science, technology, engineering, and mathematics (STEM). The primary purpose of this study was to examine whether a theoretically-grounded narrative-based intervention would be able to increase elementary school-aged girls’ self-efficacy in respect to future careers and interest in STEM subjects in school. The study sought to do this by using the stories of the “Heroes of STEM”, a series of four graphic novels developed by the principal researcher, as an intervention tool linking girls’ typically preferred subject (i.e., reading, language arts) to math and science. …

Children’S Perception Of Visual And Auditory Ambiguity And Its Link To Executive Functions And Creativity, M Taranu, Mc Wimmer, J Ross, D Farkas, Et Al

School of Psychology

The phenomenon of perceptual bistability provides insights into aspects of perceptual processing not normally accessible to everyday experience. However, most experiments have been conducted in adults, and it is not clear to what extent key aspects of perceptual switching change through development. The current research examined the ability of 6-, 8-, and 10-year-old children (N = 66) to switch between competing percepts of ambiguous visual and auditory stimuli and links between switching rate, executive functions, and creativity. The numbers of switches participants reported in two visual tasks (ambiguous figure and ambiguous structure from motion) and two auditory tasks (verbal transformation …

Master Gardener Satisfaction And Intent To Remain After Training Via Remote Delivery, Emily A. Burton, Susan S. Barton

Journal of Extension

One year after using remote delivery via video web conferencing (VWC) in training, we surveyed Delaware master gardeners to assess the remote delivery trainees' satisfaction and intent to remain as compared to those characteristics in volunteers who had had face-to-face training. Although the remote delivery trainees were satisfied overall, they were significantly less satisfied than those who had participated in face-to-face trainings. A more positive perception of the VWC experience was associated with master gardeners' increased satisfaction, suggesting the importance of VWC quality. However, volunteers trained via VWC did not report more or less of an intention to remain with …

Using Geographic Information System Technology To Identify Environmental Education Field Sites, Whitney Morgan, Rebecca Barlow

Journal of Extension

Natural resources Extension educators use outdoor environmental education sites to increase public knowledge of environmental issues, but locating appropriate sites can be time-consuming. Geographic information system (GIS) software and geospatial data can help Extension professionals choose such sites more efficiently. We describe our use of GIS technology to select a natural resources field day site in Tuskegee National Forest in Alabama. We used site characteristics such as forest type, road access, and slope to narrow potential sites from 94 possibilities to the two best options. Sources of free and low-cost geospatial data also are discussed.

Eat This In Remembrance: The Zooarchaeology Of Secular And Religious Sites In 17th-Century New Mexico, Ana C. Opishinski

Graduate Masters Theses

This thesis examines the faunal remains from LA 20,000, a 17th-century Spanish estancia near Santa Fe, New Mexico that was inhabited by a family of Spanish colonists and indigenous laborers. The data collected from these specimens are examined to better understand the diet of the site’s inhabitants, especially in conjunction with existing data on the plant portion of the diet at this site. Creating a more complete picture of the diet, the analysis covers Number of Identified Specimens (NISP), Minimum Number of Individuals (MNI), potential meat weight represented by the various species, bone modifications, and ageing and kill-off patterns. These …

Regional Variation In Grass, Sedge, And Cereal Cultivation During The Viking Age In Skagafjörður, North Iceland, Melissa M. Ritchey

Graduate Masters Theses

In Viking Age and Medieval Iceland, livestock forage was a critical resource in the Norse agropastoral economy. Cereal cultivation, typically an important part of the Norse economy, may have been more limited in marginal sub-Arctic Iceland. An analysis of macrobotanical seed assemblages from archaeological excavations at 42 Viking Age and Medieval farmsteads in the Skagafjörður region of North Iceland suggests both broad trends and substantial variation over time and space in agropastoral production practices. This study finds that the main components of livestock forage (grass, sedge, and perhaps cereal) are highly variable between regions and over time. Interestingly, barley (Hordeum …

Comparative Spending Of Medicaid Dollars On Child Participants Of Kentucky’S Sobriety Treatment And Recovery Teams Program Versus A Matched Comparison Group., Matthew Thomas Walton

Electronic Theses and Dissertations

Child protective services agencies have long observed the complicating role that parental substance use and addiction plays in cases of child maltreatment. Families who struggle with these problems present unique challenges for child welfare professionals. These families are typically more difficult to engage, more likely to have children removed from the home, and have poorer outcomes when compared to other families. These poorer outcomes often include health problems. Addiction has well-known effects on health, and the specific manifestations of these problems for parents have been documented for years in child protection casework. However, what has been less investigated are the …

Determining Readiness To Take The National Council Licensure Examination For Registered Nurses®, Heidi Elizabeth Monroe

Dissertations

Numerous studies have examined the association between pre-admission and/or during nursing program variables and the outcome on the National Council Licensure Examination for Registered Nurses (NCLEX-RN®; National Council of State Boards of Nursing [NCSBN]; 2015) but very few studies have examined post-graduation variables. This study examined post-graduation variables associated with the outcome on the NCLEX-RN® examination. The purpose of this exploratory field study was twofold. The first aspect was to identify the benchmark score on a post-graduation test (the Kaplan RN Readiness Exam; Sanders, 2014) correlated with passing the NCLEX-RN® examination. The second aspect was to explore factors that the …

The Gsk-3Β-Fbxl21 Axis Regulates Tcap Via Ubiquitin-Mediated Proteasomal Pathway In The Cytoplasm, Jiah Yang

Dissertations and Theses (Open Access)

Protein turnover is one of the most essential mechanisms controlling circadian rhythms. F-Box and Leucine Rich Repeat Protein21 (FBXL21) is a circadian E3 ligase which shows oscillatory mRNA transcripts and protein levels. It was previously found to perform subcellular compartment-specific E3 ligase activities targeting the core clock proteins CRYPTOCHROME(CRY)1/2. Here we identified a new sarcomeric target substrate, Telethonin(TCAP), which also shows circadian oscillation in its mRNA transcript and protein expression and, importantly, interaction with FBXL21 in an anti-phasic manner. Via computational and pharmacological tests, we identified Glycogen Synthase Kinase-3β(GSK-3β) as a regulator of FBXL21. Biochemical and molecular characterizations demonstrated that …

Evaluating The Therapeutic Efficacy Of Grb2 Inhibition In Ovarian Malignancies, Olivia Lara

Dissertations and Theses (Open Access)

Purpose: Adaptor proteins such as growth factor receptor-bound protein-2 (Grb2) play important roles in cancer cell signaling. In the present study, we examined the biological effects of liposomal antisense oligodeoxynucleotide that blocks Grb2 expression (L-Grb2) in ovarian cancer models.

Experimental Design: Murine orthotopic models of ovarian cancer (OVCAR5 and SKOV3ip1) were used to study the biological effects of L-Grb2 on tumor growth. In vitro experiments (cell viability assay, Western blot analysis, siRNA transfection, and reverse phase protein array) were carried out to elucidate the mechanism and potential predictors of tumor response to L-Grb2.

Results: Treatment with L-Grb2 decreased tumor growth …

Identifying And Targeting Adaptive Changes To Bet Inhibition In Ovarian Cancer, Christopher Lafargue

Dissertations and Theses (Open Access)

Purpose: To identify combination strategies using Bromodomain and Extra-Terminal Domain (BET) inhibitors in a mechanism driven fashion to maximize anti-tumor activity, and to determine the efficacy of BET inhibitor combinations in pre-clinical ovarian cancer mouse models.

Experimental Design: We used a novel, previously uncharacterized pan-BET inhibitor, CN210, for all in vitro (MTT, apoptosis, protein expression, reverse phase protein array (RPPA) analysis and in vivo (orthotopic mouse model)) experiments. The poly (ADP-ribose) polymerase (PARP) inhibitor olaparib, as well as the mechanistic target of rapamycin (mTOR) inhibitors rapamycin and INK-128 were also used. Statistical analyses of in vitro and in vivo experiments …

The Role Of Secretory Phospholipase A2 Group Iia In Obesity And Metabolism, Michael S. Kuefner

Theses and Dissertations (ETD)

Secretory phospholipase A2 group IIA (PLA2G2A) is a member of a family of secretory phospholipases previously implicated in inflammation, atherogenesis, and antibacterial actions. These enzymes hydrolyze glycerophospholipids at the sn-2 position releasing lysophospholipids and fatty acids. Though studies have shown PLA2G2A is pro-inflammatory and promotes atherosclerosis, no research has analyzed the role of this enzyme in obesity and metabolism. Studies in the past 5-10 years utilizing various knock- out or over-expression mouse models have analyzed the role of different secretory phospholipase A2s (sPLA2) in metabolic diseases. From these studies, it is known that at least seven of the 11 sPLA2 …

An Integrated Model Of Eating Disorder Risk And Protective Factors: Implications For Research And Treatment, Leslie Alison Davis-Waddle

Electronic Theses and Dissertations

Body dissatisfaction and disordered eating behaviors are significant public health concerns, especially among college-age women. Despite extensive research, the complex nature of disordered eating and its etiology has prevented a satisfactory predictive model from being developed and validated and has therefore hindered the development of effective prevention and intervention strategies. This study aimed to integrate four of the most common etiological theories into a model to predict disordered eating behaviors. Bivariate correlations and moderation analyses were conducted to determine the strength of relationships between variables and to determine whether a protective factor (i.e., self-compassion) moderates the effects of risk factors …