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Elevated Temperature Attenuates Ovarian Functions And Induces Apoptosis And Oxidative Stress In The American Oyster, Crassostrea Virginica: Potential Mechanisms And Signaling Pathways, Sarah Nash, Jackson Johnstone, Md. Saydur Rahman

School of Earth, Environmental, & Marine Sciences Faculty Publications

Global climate change is predicted to intensify thermal stress in marine and coastal organisms, affecting their development, growth, and reproductive functions. In this study, we performed histological observations on ovarian development, immunohistochemical analyses of ovarian heat shock protein-70 (HSP70), nitrotyrosine protein (NTP, an indicator of reactive nitrogen species (RNS)), and dinitrophenyl protein (DNP, an indicator of protein oxidation) expressions, in situ TUNEL assay for cellular apoptosis, biochemical analyses of ovarian caspase-3/7 activity and protein carbonyl (PC, a measure of reactive oxygen species (ROS)) contents, nitrate/nitrite (NOx) levels, and extrapallial fluid (EPF, an important body fluid) pH in the American oyster, …

Don Reduction Of Wheat Grain Without Compromising The Lab-Scale Milling Properties Of Flour, Jikai Zhao, Xin Liu, Fengcheng Wang

School of Earth, Environmental, & Marine Sciences Faculty Publications

Wheat bran was investigated to be the most commonly contaminated raw material by mycotoxins. However, there are no economical and practical pretreatment methods for industrial on-line application until now. The effect of light debranning on deoxynivalenol (DON) removal, polyphenol oxidase (PPO) activity and flour quality from lab-scale milling were performed. For on-line production, the DON concentration in wheat decreased 15.89% at debranning ratio of 1.2%. For lab experiment, the maximum DON removal for wheat and flour was 23.35% and 21.95%, respectively. However, the PPO activity, browning of dough sheet and flour qualities in lab scale exhibited no significant variation. …

Regulatory Mechanisms Mediated By Peroxisome Proliferator-Activated Receptor-Β/Δ (Pparβ/Δ) In Skin Cancer, Jeffrey M. Peters, Dae Joon Kim, Moses T. Bility, Michael G. Borland, Bokai Zhu, Frank J. Gonzalez

School of Medicine Publications

Considerable progress has been made during the past twenty years towards elucidating the role of peroxisome proliferator-activated receptor-β/δ (PPARβ/δ) in skin cancer. In 1999, the original notion that PPARβ/δ was involved with epithelial cell function was postulated based on a correlation between PPARβ/δ expression and the induction of mRNAs encoding proteins that mediate terminal differentiation in keratinocytes. Subsequent studies definitively revealed that PPARβ/δ could induce terminal differentiation and inhibit proliferation of keratinocytes. Molecular mechanisms have since been discovered to explain how this nuclear receptor can be targeted for preventing and treating skin cancer. This includes the regulation of terminal differentiation, …

Obstetric And Neonatal Complications Among Women With Autoimmune Disease, Andrew Williams, Katherine Grantz, Indulaxmi Seeni, Candace Robledo, Shanshan Li, Marion Ouidir, Carrie Nobles, Pauline Mendola

School of Medicine Publications

Background: The impact of autoimmune diseases on pregnancy remains understudied on a population level. Examination of obstetric and neonatal outcomes among women with autoimmune disease and their infants can provide important insights for clinical management.

Methods: Autoimmune diseases and outcomes were identified using medical records. Cesarean delivery, preterm birth, preeclampsia, small for gestational age (SGA), neonatal intensive care (NICU) admission, neonatal respiratory distress syndrome (RDS), and perinatal mortality risk was assessed. Poisson regression with robust standard errors estimated relative risks (RR) and 95% confidence intervals (95% CI) with adjustment for maternal characteristics and other chronic conditions.

Results: Women with …

Laser-Induced Breakdown Spectroscopy (Libs): A Potential Quality Tool For Infant Formula Manufacture, Xavier Cama Moncunill

Doctoral

Breast milk is considered the ideal food for infants. However, breastfeeding is at times supplemented or replaced with suitable alternatives. Infant formula (IF) is an industrially produced food intended as a breast milk substitute, which needs to satisfy, by itself, all nutritional requirements of infants. For this reason, it is of critical importance that IF provides the adequate amounts of nutrients including carbohydrates, fats, proteins, minerals and vitamins. Laser-induced breakdown spectroscopy (LIBS) is a promising emission spectroscopic technique for elemental analysis, which can provide real-time measurements with little to no sample preparation. Hence, the main goal of this thesis was …

Integration Of Suboptimal Health Status Evaluation As A Criterion For Prediction Of Preeclampsia Is Strongly Recommended For Healthcare Management In Pregnancy: A Prospective Cohort Study In A Ghanaian Population, Enoch Odame Anto, Peter Roberts, David Coall, Cornelius Archer Turpin, Eric Adua, Youxin Wang, Wei Wang

Research outputs 2014 to 2021

Background: Normotensive pregnancy may develop into preeclampsia (PE) and other adverse pregnancy complications (APCs), for which the causes are still unknown. Suboptimal health status (SHS), a physical state between health and disease, might contribute to the development and progression of PE. By integration of a routine health measure in this Ghanaian Suboptimal Health Cohort Study, we explored the usefulness of a 25-question item SHS questionnaire (SHSQ-25) for early screening and prediction of normotensive pregnant women (NTN-PW) likely to develop PE. Methods: We assessed the overall health status among a cohort of 593 NTN-PW at baseline (10–20 weeks gestation) and followed …

Air Pollution, Neighborhood Deprivation, And Autism Spectrum Disorder In The Study To Explore Early Development, Laura A. Mcguinn, Gayle C. Windham, Lynne C. Messer, Di Qian, Joel Schwartz, Lisa A. Croen, Eric J. Moody, Ana G. Rappold, Multiple Additional Authors

OHSU-PSU School of Public Health Faculty Publications and Presentations

Background: To examine whether neighborhood deprivation modifies the association between early life air pollution exposure and autism spectrum disorder (ASD), we used resources from a multisite case–control study, the Study to Explore Early Development.

Methods: Cases were 674 children with confirmed ASD born in 2003–2006; controls were 855 randomly sampled children born during the same time period and residents of the same geographic areas as cases. Air pollution was assessed by roadway proximity and particulate matter

Results: Neighborhood deprivation modified (P_{for interaction} = 0.08) the association between PM2.5 exposure during the first year of life …

The Ace1 Electrical Impedance Tomography System For Thoracic Imaging, Michelle M. Mellenthin, Jennifer L. Mueller, Erick Dario Leon Bueno De Camargo, Fernando Silva De Moura, Talles Batista Rattis Santos, Raul Gonzales Lima, Sarah J. Hamilton, Peter A. Muller, Melody Alsaker

Mathematical and Statistical Science Faculty Research and Publications

The design and performance of the active complex electrode (ACE1) electrical impedance tomography system for single-ended phasic voltage measurements are presented. The design of the hardware and calibration procedures allows for reconstruction of conductivity and permittivity images. Phase measurement is achieved with the ACE1 active electrode circuit which measures the amplitude and phase of the voltage and the applied current at the location at which current is injected into the body. An evaluation of the system performance under typical operating conditions includes details of demodulation and calibration and an in-depth look at insightful metrics, such as signal-to-noise ratio variations during …

Centeringpregnancy: Perceptions Of Providers And Staff In A Private Ob Practice, Allison F. Mills

Doctoral Dissertations and Projects

Traditional prenatal care has been the stalwart of care in the United States since the 1920s; however, a new model of care is emerging: group-style prenatal care. This model of care has been well-documented within literature as having notable maternal and fetal outcomes, including increased patient satisfaction, decreased preterm birth rates, increased breastfeeding rates, and increased patient compliance, to name only a few. With such remarkable outcomes, it begs the question of why the group prenatal care model is not more widely utilized. This project aimed to determine if increasing the knowledge of healthcare providers in a private obstetrics practice …

Depression In Black Men: One Church’S Solution, Dwayne T. Baskin

Doctoral Dissertations and Projects

This dissertation examines the Black Church’s influence on Pentecostal African-American men who are depressed, and how the church may assist these men to heal from the wounds of despair through a Pentecostal experience. While many Pentecostal African-American men have matriculated through the ranks of leadership, establishing successful businesses, churches, ministries, and organizational denominations; they are teetering on the edge of an emotional and spiritual breakdown. Researchers have found that African-American men are understudied and underdiagnosed as it pertains to depression. Eight African-American Pentecostal men were interviewed and given questionnaires to examine how depression affected them while maintaining leadership roles in …

Reading Habits And Preferences Of Female Collegiate Students From Jalandhar Region: A Survey, Shaveta Azad

Library Philosophy and Practice (e-journal)

Abstract –Read in order to know won’t be wrong if we talk about the importance of reading in a student’s life during his/her academic career and later. Reading makes one capable of being a lifelong learner through updating and expanding one’s existing knowledge. Unfortunately, Indian Education System do not comprise of special types of Reading programs for students to nurture the habits of reading since their very early age , like other developed countries. The present study aims to find out the reading habits of college going female students studying in different streams such as Humanities, Non-Med, Pure Sciences, …

User Satisfaction Of Resources And Services Of Libraries: A Case Study Of The Narh-Bita College, Tema, Ghana., Zakaria Abukari

Library Philosophy and Practice (e-journal)

Information and communication technologies have revolutionized how information is delivered to information consumers in the 21^st century. Information can now be obtained just with a click of a button. For this reason, libraries are faced with the challenge of maintaining their clientel base. Therefore, user satisfaction of libraries' resources and services has become the major focus for most libraries. User satisfaction has been found to be an important factor that affects the use or non use of libraries. The study investigated the utilization and user satisfaction of the resources and services of the Narh-Bita College Library, Ghana. The study …

Investigations Of Point Defects In Kh2Po4 Crystals Using Ab Initio Quantum Methods, Tabitha E. R. Dodson

Theses and Dissertations

Potassium dihydrogen phosphate (KH₂PO₄, or commonly called KDP) crystals can be grown to large sizes and are used for many important devices (fast optical switches, frequency conversion, polarization rotation) for high powered lasers. The nonlinear optical material has a wide intrinsic transparency range. Intrinsic point defects are responsible for several short-lived absorption bands in the visible and ultraviolet regions that affect high-power pulsed laser propagation. The primary intrinsic defects have been experimentally detected in KDP using electron paramagnetic resonance (EPR) experiments. The defect models established thus far include (i) self-trapped holes, (ii) oxygen vacancies, and (iii) …

Bias-Corrected Maximum-Likelihood Estimator For The Parameter Of The Logarithmic Series Distribution And Its Characterizations, Mahdi Rasekhi, Gholamhossein Hamedani

Mathematical and Statistical Science Faculty Research and Publications

In this article, we study parameter estimation of the logarithmic series distribution. A well-known method of estimation is the maximum likelihood estimate (MLE) and this method for this distribution resulted in a biased estimator for the small sample size datasets. The goal here is to reduce the bias and root mean square error of MLE of the unknown parameter. Employing the Cox and Snell method, a closed-form expression for the bias-reduction of the maximum likelihood estimator of the parameter is obtained. Moreover, the parametric Bootstrap bias correction of the maximum likelihood estimator is studied. The performance of the proposed estimators …

Hydrodynamic And Total Dissolved Solids Model Of The Tigris River Using Ce-Qual-W2, Muhanned D. Al-Murib, Scott Wells

Civil and Environmental Engineering Faculty Publications and Presentations

The headwaters of the Tigris River basin in Iraq is controlled by Turkey due to a series of dams constructed over the last few decades. Since Total Dissolved Solids (TDS) in the Tigris River within Baghdad and downstream cities can reach 1000 mg/L exceeding both drinking water and irrigation guidelines, a hydrodynamic and water quality model, CE-QUAL-W2, of the river was developed to understand how changes in flow affect TDS downstream. A model of 880 km of the Tigris River from Mosul Dam to Kut Barrage including Tharthar Lake was constructed for 2009. Model development was challenging due to a …

An Exploratory Investigation About Graduate Counseling Students’ Perceived Competencies In Integrated Care, Bridget Asempapa

Journal of Counselor Preparation and Supervision

Integrated care is a holistic treatment modality that increases collaborative efforts between primary care and mental health providers. Support for integrated care is evident, but the development of counselors for this level of care is limited. This study examined graduate counseling students’ (N = 243) perceived competencies about integrated care. Additionally, the study shares findings regarding students’ experiences, useful skills, and concerns about their work in integrated care settings. The results from this study suggest that students have encouraging perceived competencies. However, there were indications that specific contextual challenges remain, but exposure to integrated care training will be valuable. Implications …

Mental Health Professionals’ Attitudes Toward Clients With Antisocial Personality Disorder: An Exploratory Study, Edward T. Dunbar Jr., Shari M. Sias, Stephen Leierer, William L. Atherton, Robert J. Campbell, Lloyd R. Goodwin Jr

Journal of Counselor Preparation and Supervision

This exploratory study examined mental health professionals’ attitudes toward clients with antisocial personality disorder. Specifically, are mental health professionals’ attitudes influenced by (a) personal experiences with criminal victimization, or (b) contact with clients with antisocial personality disorder. A factorial MANOVA and follow-up univariate ANOVAs revealed a statistically significant main effect in relation to participants’ level of clinical contact with clients having antisocial personality disorder. Participants with higher levels of clinical contact were associated with more positive attitudes towards clients. Implications for mental health professionals, supervisors, and counselor educators are discussed, and suggestions for future research are provided.

Neotype Designation For Pectinibuthus Birulai Fet, 1984 (Scorpiones: Buthidae) From Turkmenistan, With Remarks On Pectine Teeth Of Psammophile Scorpions, Victor Fet, František Kovařík, Graeme Lowe

Euscorpius

A neotype is designated for a very rare Central Asian scorpion, Pectinibuthus birulai Fet, 1984, the sole species of the genus Pectinibuthus Fet, 1984. It is the only currently known specimen, collected by Victor Fet in July 1985, and deposited in ZISP (St. Petersburg, Russia). The original types are considered lost. Detailed photographs of the neotype are provided, as well as comments on this unique psammophile buthid. We also discuss and compare pectinal tooth counts of psammophile scorpions relative to other scorpions.

Developmental And Sex Modulated Neurological Alterations In Autism Spectrum Disorder, Azeezat Azeez

Dissertations

Autism Spectrum Disorder (ASD) was first described in 1943 by Dr. Leo Kranner in a case study published in The Nervous Child. It is a neurodevelopment disorder, with a range of clinical symptoms. According to the Diagnostic and Statistical Manual of Mental Disorders (DSM-5), used by clinicians to diagnose mental disorders, a child needs to have persistent social deficits, language impairments, and repetitive behaviors, that cannot be explained by neurological damage or intellectual disability. It is known that children diagnosed with ASD are often are developmentally delayed therefore alterations in the typical developmental trajectory should be a major factor in …

Modulation Of Corticospinal Excitability Induced By Paired Associative Stimulation Combined With Movement, Ahmad O. Alokaily

Dissertations

An essential feature of the brain is its capacity to undergo long-lasting morphological or functional changes in response to experiences or trauma. Advances in noninvasive brain stimulation techniques have led to increased interest in understanding neural mechanisms of neuroplasticity at the network level. Paired associative stimulation (PAS) is one of the most commonly used applications for noninvasive brain stimulation because of its clinical potential as an adjuvant rehabilitative intervention. However, the optimal method for incorporating PAS into rehabilitative activities remains unknown. This dissertation explores different approaches to combining PAS with movement and investigates the enhancement of the specificity of conventional …

Modelling In Vitro Dissolution And Release Of Sumatriptan Succinate From Polyvinylpyrrolidone-Based Microneedles Aided By Iontophoresis, James Paul Ronnander

Dissertations

A novel dissolving microneedle array system is developed to investigate permeation of a sumatriptan succinate formulations through the skin aided by iontophoresis. Three formulations consisting of hydrophilic, positively charged drug molecules encapsulated in a water-soluble biologically suitable polymer, polyvinylpyrrolidone (PVP), have been accepted by the U.S. Food and Drug Administration (FDA). The microneedle systems are fabricated with 600 pyramid-shaped needles, each 500 µm tall, on a 0.785-cm² circular array. In vitro transdermal studies with minipig skin and vertical Franz diffusion cells show > 68% permeation of sumatriptan over a 24-hour period. A combination of microneedle and electrical current density ranging …

Engineering Of Escherichia Coli 2-Oxoglutarate Dehydrogenase Complex With Mechanistic And Synthetic Goals, Joydeep Chakraborty

Dissertations

The Escherichia coli 2-oxoglutarate dehydrogenase complex (OGDHc) compromises multiple copies of three enzymes - 2-oxoglutarate dehydrogenase (E1o), dihydrolipoyl succinyltransferase (E2o), and dihydrolipoyl dehydrogenase (E3). OGDHc is found in the Krebs cycle and catalyzes the formation of the all-important succinyl-Coenzyme A (succinyl-CoA). OGDHc was engineered to understand the catalytic mechanism and optimized for chemical synthetic goals.

Succinyl-CoA formation takes place within the catalytic domain of E2o via a transesterification reaction. The succinyl group from the thiol ester of S8-succinyldihydrolipoyl-E2o is transferred to the thiol group of CoA. Mechanistic studies were designed to investigate enzymatic transthioesterification. His375 and Asp374 was shown to …

Analyzing Evolution Of Rare Events Through Social Media Data, Xiaoyu Lu

Dissertations

Recently, some researchers have attempted to find a relationship between the evolution of rare events and temporal-spatial patterns of social media activities. Their studies verify that the relationship exists in both time and spatial domains. However, few of those studies can accurately deduce a time point when social media activities are most highly affected by a rare event because producing an accurate temporal pattern of social media during the evolution of a rare event is very difficult. This work expands the current studies along three directions. Firstly, we focus on the intensity of information volume and propose an innovative clustering …

Optimal Sampling Paths For Autonomous Vehicles In Uncertain Ocean Flows, Andrew J. De Stefan

Dissertations

Despite an extensive history of oceanic observation, researchers have only begun to build a complete picture of oceanic currents. Sparsity of instrumentation has created the need to maximize the information extracted from every source of data in building this picture. Within the last few decades, autonomous vehicles, or AVs, have been employed as tools to aid in this research initiative. Unmanned and self-propelled, AVs are capable of spending weeks, if not months, exploring and monitoring the oceans. However, the quality of data acquired by these vehicles is highly dependent on the paths along which they collect their observational data. The …

Graphene Channels Interfaced With Distributed Quantum Dots, Xin Miao

Dissertations

Previous research has elucidated the remarkable electrical and optical characteristics of graphene and pointed to the various applications of graphene-based devices. One of such applications is electro-optical graphene-based elements. In this work, the optoelectronic properties of field-effect transistors are explored. These are composed of surface graphene guides, which are interfaced with an array of individual semiconductor quantum dots. The graphene guide also serves as a channel for the field-effect transistor (FET) while the dots provide for fluorescence markers. They may be placed either within the capacitor formed between the graphene and the gate electrode, or on top of the graphene. …

A Role Of Ubiquitin Regulatory X-Domain Containing Proteins (Ubxn6) In Antiviral Immunity, Harshada Ketkar, Harshada Ketkar

NYMC Student Theses and Dissertations

The roles of UBXNs in the regulation of antiviral immune responses have not been much explored. Previous work in our lab identified UBXN1 as a negative regulator of the retinoic acid-inducible gene-I (RIG-I) like receptors (RLR) pathway and UBXN3B as a positive regulator of stimulator-of-interferon Genes (STING) -mediated immune responses. In this study, I aimed to determine the member of UBXNs as a positive regulator of ribonucleic acid (RNA) virus infection-induced innate immune responses. By using an interferon stimulated response element (ISRE)-driven luciferase reporter assay that monitors the activity of type I/III interferon (IFN)-induced janus kinase (JAK) - signal transducer …

Hematological Indices Of Pesticide Exposure Rice Farmers In Southern Thailand, Wiyada Kwanhian, Supabhorn Yimthiang, Siriuma Jawjit, Junjira Mahaboon, Udomratana Vattanasit, Phiman Thirarattanasunthon

Kesmas

The most common pesticides used in rice farming are organophosphates and carbamates. These pesticides inhibit acetylcholinesterase (AChE) activity, resulting in excessive levels of acetylcholine accumulation and disrupted neurotransmission. This study compared AChE activity and hematological parameters between pesticide-using rice farmers, non-pesticide using rice farmers, and non-agricultural occupational groups (non-farmers). Pesticide residues that accumulated in rice and the water and soil of the study area were also determined. AChE activity of all participants showed 10 from 87 samples (11.49%) as borderline depressed, with 50% of these being pesticide-using farmers. Most of the hematological parameters were normal in all samples. However, platelet …

5 Lipoxygenase's Role In Chondrogenesis Of The Atdc5 Cell Line, Tien My Tran

Seton Hall University Dissertations and Theses (ETDs)

5-Lipoxygenase metabolizes arachidonic acid (AA) to generate leukotrienes (LTs). Leukotrienes are very potent inflammatory mediators that can have negative effects on bone healing and cartilage tissue generation. Recent studies have shown that decreasing 5-lipoxygenase (5-LO) activity during fracture healing accelerates endochondral ossification and decreases healing time in animal fracture models, however, the roles of 5-LO in chondrogenesis is not fully investigated. In this study, we tested the hypothesis that 5-LO inhibition will increase the process of chondrogenesis. The ABT-761 is an N-hydroxyurea analog structurally similar to zileuton, which is a 5-LO enzyme inhibitor, The ATDC5 cell line which is derived …

Neonicotinoid Insecticide Concentrations In Agricultural Wetlands And Associations With Aquatic Invertebrate Communities, Travis J. Schepker, Elisabeth B. Webb, Donald Tillitt, Ted Lagrange

Nebraska Game and Parks Commission: Staff Research Publications

Neonicotinoids are considered a superior insecticide for agricultural pest management, although their impacts on non-target insects is a rising concern. Aside from laboratory and mesocosm studies, limited research has been directed towards the role neonicotinoids may have in structuring aquatic invertebrate communities in field settings. Therefore, we simultaneously collected aquatic invertebrate and surface water samples from 26 wetlands within a highly modified agricultural landscape of Nebraska’s Rainwater Basin during spring 2015. Water samples were tested for six different neonicotinoids, nutrients, and physical properties. Trace levels of clothianidin and imidacloprid were the only neonicotinoids detected, occurring in 85% and 15%, respectively, …

The “Rights” Of Precision Drug Development For Alzheimer’S Disease, Jeffrey Cummings, Howard H. Feldman, Philip Scheltens

School of Medicine Faculty Research

There is a high rate of failure in Alzheimer’s disease (AD) drug development with 99% of trials showing no drug-placebo difference. This low rate of success delays new treatments for patients and discourages investment in AD drug development. Studies across drug development programs in multiple disorders have identified important strategies for decreasing the risk and increasing the likelihood of success in drug development programs. These experiences provide guidance for the optimization of AD drug development. The “rights” of AD drug development include the right target, right drug, right biomarker, right participant, and right trial. The right target identifies the appropriate …