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2019

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Effects Of Aquatic Acidification On Calcium Uptake In White River Shrimp Litopenaeus Setiferus Gills, Maria-Flora Jacobs Jan 2019

Effects Of Aquatic Acidification On Calcium Uptake In White River Shrimp Litopenaeus Setiferus Gills, Maria-Flora Jacobs

UNF Graduate Theses and Dissertations

Previous research regarding aquatic acidification has examined the protonation of the carbonate and does not consider calcium to be a limiting factor. This is the first study to suggest that pH may affect the uptake of calcium in crustacean gills. This project describes ion transport mechanisms present in the cell membranes of white river shrimp Litopenaeus setiferus gill epithelium, and the effects of pH on the uptake of calcium by these means. Partially purified membrane vesicles (PPMV) of shrimp gills were prepared through a homogenization process that has been used previously to define ion transport in crab and lobster gill …


Generation Z'S Positive And Negative Attributes And The Impact On Empathy After A Community-Based Learning Experience, Amanda Nicole Moscrip Jan 2019

Generation Z'S Positive And Negative Attributes And The Impact On Empathy After A Community-Based Learning Experience, Amanda Nicole Moscrip

UNF Graduate Theses and Dissertations

Generation Z, also known as the iGeneration, iGenners, GenZ, and Generation Now, consists of those born in the mid-1990s through the late 2010s. Historical events important for this generation have influenced their perception of safety as well as how they interact with others. As compared to previous generations, technological advances (i.e., Smartphones, social media) changed how GenZ communicates, socializes, and receives information. Unique experiences and attributes influenced Generation Z’s empathy because living through these events and seeing their impact changes how they can understand and take the perspective of others. The relation between three factors was examined across University students …


Experimental Analysis Of Protective Headgear Used In Defensive Softball Play, John Scott Strickland Jan 2019

Experimental Analysis Of Protective Headgear Used In Defensive Softball Play, John Scott Strickland

UNF Graduate Theses and Dissertations

Every year in the United States, an estimated 1.6 to 3.8 million people sustain sports-related traumatic brain injuries (TBIs), with an appreciable number of these injuries coming from the sport of softball. Several studies have analyzed the impact performance of catcher’s masks within the context of baseball; however, virtually no studies have been performed on fielder’s masks within the context of softball. Thus, the main objective of the present work was to evaluate the protective capabilities of softball fielder’s masks. To better understand the injury mechanisms and frequency associated with softball head/facial injuries, epidemiological data from a national database was …


An Fmri Exploration Of Information Processing In Electronic Networks Of Practice, Thomas O. Meservy, Kelly J. Fadel, C. Brock Kirwan, Rayman D. Meservy Jan 2019

An Fmri Exploration Of Information Processing In Electronic Networks Of Practice, Thomas O. Meservy, Kelly J. Fadel, C. Brock Kirwan, Rayman D. Meservy

Faculty Publications

Online forums sponsored by electronic networks of practice have become an important source of information for individuals seeking solutions to problems online. However, not all information available in a forum is helpful or accurate, requiring knowledge seekers to evaluate and filter the solutions they encounter. Most forums offer contextual cues to help knowledge seekers make evaluation decisions, yet little is understood about the cognitive processes and neural mechanisms that underlie how information on these forums is filtered and evaluated. This paper draws on literature in cognitive neuroscience and NeuroIS to develop exploratory research questions about the role of both content …


Moral Development In Business Ethics: An Examination And Critique, Kristen Bell Detienne, Carol Frogley Ellertson, Marc-Charles Ingerson, William R. Dudley Jan 2019

Moral Development In Business Ethics: An Examination And Critique, Kristen Bell Detienne, Carol Frogley Ellertson, Marc-Charles Ingerson, William R. Dudley

Faculty Publications

The field of behavioral ethics has seen considerable growth over the last few decades. One of the most significant concerns facing this interdisciplinary field of research is the moral judgment-action gap. The moral judgment-action gap is the inconsistency people display when they know what is right but do what they know is wrong. Much of the research in the field of behavioral ethics is based on (or in response to) early work in moral psychology and American psychologist Lawrence Kohlberg’s foundational cognitive model of moral development. However, Kohlberg’s model of moral development lacks a compelling explanation for the judgment-action gap. …


Unclogging The Pipeline: Advancement To Full Professor In Academic Stem, Helga Van Miegroet, Christy Glass, Ronda R. Callister, Kimberly Sullivan Jan 2019

Unclogging The Pipeline: Advancement To Full Professor In Academic Stem, Helga Van Miegroet, Christy Glass, Ronda R. Callister, Kimberly Sullivan

Ecology Center Publications

Purpose: Women remain underrepresented in academic STEM, especially at the highest ranks. While much attention has focused on early-career attrition, mid-career advancement is still largely understudied and undocumented. This paper analyzes gender differences in advancement to full professor within academic STEM at a mid-size public doctoral university in the western US, before and after the NSF-ADVANCE Program (2003-2007).

Methodology: Using faculty demographics and promotion data between 2008 and 2014, combined with faculty responses to two waves of a climate survey, the magnitude and longevity of the impact of ADVANCE on mid-career faculty advancement across gender is evaluated.

Findings: This study …


Detecting Soil Macrofauna Using Ground-Penetrating Radar, Melanie N. Stock, David J. Hart, Nicholas J. Balster Jan 2019

Detecting Soil Macrofauna Using Ground-Penetrating Radar, Melanie N. Stock, David J. Hart, Nicholas J. Balster

Plants, Soils, and Climate Faculty Publications

Fossorial amphibians spend up to ten months belowground, but research into this critical habitat has been impeded by a lack of noninvasive detection methods. Ground-penetrating radar (GPR), however, offers a promising tool because amphibians have theoretically strong electromagnetic (EM) contrasts relative to the soil matrix, and thus potentially high detectability. The objectives of this study were to (1) evaluate GPR by (2) experimentally-inducing three soil thermal regimes that promote stratification in the burrowing depths of 15 Eastern American Toads (Anaxyrus americanus americanus) during the winter of 2011–2012 in Madison, WI, USA. We calculated reflectability and established the unique …


Effects Of Various Toxeat$^{®}$ Concentrations On Growth Performance, Immune Response, Cecal Microflora, And Gut Morphology In Broilers Fed With Aflatoxin Contaminated Diets, Soheila Sarrami, Parvaneh Jafari, Maryam Tajabadi Ebrahimi, Seyed Naser Mousavi, Seyed Abdollah Hosseini Jan 2019

Effects Of Various Toxeat$^{®}$ Concentrations On Growth Performance, Immune Response, Cecal Microflora, And Gut Morphology In Broilers Fed With Aflatoxin Contaminated Diets, Soheila Sarrami, Parvaneh Jafari, Maryam Tajabadi Ebrahimi, Seyed Naser Mousavi, Seyed Abdollah Hosseini

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to investigate the effects of Toxeat$^{®}$, a mycotoxin binder, on functional indices, immune response, intestinal microflora, and gut morphology in broiler chickens fed aflatoxin-contaminated diets. Three hundred Cobb 500 broilers were randomly divided into 6 treatments with 5 replicates, each with 10 birds. The treatments included T1: negative control (NC), T$_{2:}$ T1 + 1 g/ton aflatoxin (positive control: PC), T$_{3:}$ PC + 1 kg/ton Toxeat$^{®}$, T$_{4:}$ PC + 2 kg/ton Toxeat$^{®}$, T$_{5:}$ PC + 3 kg/ton Toxeat$^{®}$, T$_{6:}$ PC + 1 kg/ton Toxeat$^{®}$ without hydrated sodium calcium aluminosilicate. Growth performance, immune system response, and lactic acid …


Genetic Analysis Of Iranian Mycoplasma Gallisepticum Isolates Using Gene-Targeted Sequencing Of Complete Pvpa Gene, Naghmeh Kabiri, Mohsen Bashashati, Mehdi Vasfi Marandi, Mansour Banani, Fereshteh Sabouri Jan 2019

Genetic Analysis Of Iranian Mycoplasma Gallisepticum Isolates Using Gene-Targeted Sequencing Of Complete Pvpa Gene, Naghmeh Kabiri, Mohsen Bashashati, Mehdi Vasfi Marandi, Mansour Banani, Fereshteh Sabouri

Turkish Journal of Veterinary & Animal Sciences

This study aims to evaluate Mycoplasma gallisepticum (MG) genetic diversity through the complete sequencing of the pvpA gene among six MG isolates present in industrial poultry from 2015 to 2016. The genetic comparison of these six isolates showed similarities of over 94% and 92% in terms of nucleotide and amino acid sequences, respectively. The size of the PvpA protein in the studied isolates included three different sizes with 361, 364, and 366 amino acids. The carboxy-terminus of the studied PvpA proteins contained 11 tetrapeptides of P-R-P-M/Q/N motifs with truncation in the proline-rich region. Based on the BLAST analysis, the pvpA …


Genetic Appraisal Of Growth Traits In Iranian Native Ghezel Sheep Using Random Regression Models, Bashir Ghahri, Sadegh Alijani, Seyed Abbas Rafat, Reza Nabavi, Hossein Daghigh Kia Jan 2019

Genetic Appraisal Of Growth Traits In Iranian Native Ghezel Sheep Using Random Regression Models, Bashir Ghahri, Sadegh Alijani, Seyed Abbas Rafat, Reza Nabavi, Hossein Daghigh Kia

Turkish Journal of Veterinary & Animal Sciences

This research was carried out to estimate the genetic parameters of the body weight traits of the Ghezel sheep using three different random regression models. The used data were 39,288 test-day records collected from 13,378 animals at Ghezel Sheep Breeding Station in Miandoab, West Azerbaijan Province, Iran from 1994 to 2012. Fixed effects in models included type of birth (single, twin) and year of birth (1994-2012). The weight as a function of age in the days of weighting was considered as the fixed regression of orthogonal polynomial. The random effects of the basic model were direct genetic and direct permanent …


Isolation And Molecular Characterization Of Salmonella Enterica And Escherichia Coli From Poultry Samples, Ayli̇n Cesur, Saci̇de Özlem Ulutaş, Yeşi̇m Soyer Jan 2019

Isolation And Molecular Characterization Of Salmonella Enterica And Escherichia Coli From Poultry Samples, Ayli̇n Cesur, Saci̇de Özlem Ulutaş, Yeşi̇m Soyer

Turkish Journal of Veterinary & Animal Sciences

Innumerable foodborne pathogens including Salmonella and Escherichia coli pose a serious threat to human health and food safety. As perishable foods, poultry products are considered one of the most common sources of foodborne pathogens including Salmonella and E. coli due to transmission of drug resistance, dissemination of organisms, and cross-contamination. In our study, phenotypic and genotypic characterizations of Salmonella enterica and E. coli isolated from packaged raw chicken products were carried out. Samples belonging to different commercial brands were collected in Ankara in 2015. Among 15 out of 19 Salmonella enterica subsp. enterica strains isolated from different and/or same poultry …


Analysis Of Spermatogenesis In Young Male Hybrids Bred From Domestic Cattle(Bos Taurus) And European Bison (Bison Bonasus), Andrzej Zyczynski, Marta Kloch, Wanda Olech Jan 2019

Analysis Of Spermatogenesis In Young Male Hybrids Bred From Domestic Cattle(Bos Taurus) And European Bison (Bison Bonasus), Andrzej Zyczynski, Marta Kloch, Wanda Olech

Turkish Journal of Veterinary & Animal Sciences

A new attempt was undertaken to check the reproductive capabilities of male hybrids bred from European bison (Bison bonasus) and domestic cattle (Bos taurus). The analysis concerned two hybrids aged 1.5?2.5 years (the latter being sampled twice at 6-month intervals), and additionally one 1.5-year-old backcross hybrid. Examination of testicular tissue revealed halting of spermatogenesis, which was hardly ever able to proceed further than meiosis I. Spermatogenesis was arrested at the stage of spermatogonia and primary spermatocytes. Spermatozoa were found only in the case of one hybrid and these were mostly malformed. To summarize, all the males studied were sterile, despite …


In Vitro Effect Of Recombinant Human Gonadotropins On Meiotic Competence Of Dog Oocytes, Kamber Demi̇r, Mi̇that Evecen, Ramazan Arici, Seli̇n Yağcioğlu, Nur Ersoy, Ni̇lhan Coşkun, Hatem Atalla, Kemal Ak, Sema Bi̇rler, Serhat Pabuccuoğlu Jan 2019

In Vitro Effect Of Recombinant Human Gonadotropins On Meiotic Competence Of Dog Oocytes, Kamber Demi̇r, Mi̇that Evecen, Ramazan Arici, Seli̇n Yağcioğlu, Nur Ersoy, Ni̇lhan Coşkun, Hatem Atalla, Kemal Ak, Sema Bi̇rler, Serhat Pabuccuoğlu

Turkish Journal of Veterinary & Animal Sciences

The reproductive biology of the domestic dog is unique among mammalian species; because of this, in vitro maturation (IVM) rate is still very low compared with other domestic animals in spite of attempts at improvement. The aim of this study was to consider the use of recombinant human gonadotropins as a replacement for pituitary gonadotropins for IVM of dog oocytes. A total of 845 cumulus-oocyte complexes were used in this study. To determine the effects of human recombinant gonadotropins, maturation medium was supplemented with two different concentrations (0.05 or 0.1 IU/mL) of pituitary (pFSH, pLH) and human recombinant (rhFSH and …


The Effects Of Supplementation Of Rumen-Protected Choline On Some Blood And Milk Metabolites In The Transition Period Of Dairy Cattle, Ni̇yazi̇ Alan, Mustafa Salman Jan 2019

The Effects Of Supplementation Of Rumen-Protected Choline On Some Blood And Milk Metabolites In The Transition Period Of Dairy Cattle, Ni̇yazi̇ Alan, Mustafa Salman

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to investigate nonesterified fatty acid (NEFA), beta-hydroxy butyric acid (BHBA), and milk urea nitrogen (MUN) profiles for dairy cows fed with rumen-protected choline (RPC) during the transition period. A total of 30 dairy cows were allocated to one control and two experimental groups (10 animals per group) and were fed from 3 weeks before calving to 21 days in milk. The control was fed only the basal diet. Treatment groups 1 and 2 were fed a basal diet containing 60 and 120 g/head/day RPC, respectively. The differences between groups were insignificant for MUN levels …


Methods Of Diagnosis And Treatment Of Postpartum Uterine Infection Adopted By Algerian Veterinarians, Hamza Rahab, Alessandro Ricci, Ahmed Kasseh-Laouar, Christian Hanzen Jan 2019

Methods Of Diagnosis And Treatment Of Postpartum Uterine Infection Adopted By Algerian Veterinarians, Hamza Rahab, Alessandro Ricci, Ahmed Kasseh-Laouar, Christian Hanzen

Turkish Journal of Veterinary & Animal Sciences

The present study aimed to monitor the practices of Algerian veterinarians in the diagnosis and treatment of puerperal metritis (PM) and clinical endometritis (CE) in cattle. For this purpose, a questionnaire was established and distributed to collect the information concerning veterinarian profiles and practices related to the diagnosis and treatment of PM and CE. The results showed that examinations of the uterus (rectal palpation), vaginal discharge, and rectal temperature were the most practiced methods for PM (71%, 68%, and 83%, respectively) and CE (86%, 60%, and 65%, respectively) diagnosis. Tetracycline was the antibiotic of choice for intrauterine treatment of PM …


Comparative Detection Of Bovine Herpesvirus-1 Using Antigen Elisa, Immunohistochemistry And Immunofluorescence Methods In Cattle With Pneumonia, Seli̇m Çomakli, Yavuz Seli̇m Sağlam, Mehmet Özkan Ti̇murkan Jan 2019

Comparative Detection Of Bovine Herpesvirus-1 Using Antigen Elisa, Immunohistochemistry And Immunofluorescence Methods In Cattle With Pneumonia, Seli̇m Çomakli, Yavuz Seli̇m Sağlam, Mehmet Özkan Ti̇murkan

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to detect the prevalence and presence of bovine herpesvirus-1 (BoHV-1) using immunohistochemistry (IHC), immunofluorescence (IF) and antigen enzyme-linked immunosorbent assay (ELISA) in the lung samples of cattle (n = 1023). In addition, three methods were compared using receiver operating characteristic (ROC) and chi-square test ($χ^{2}$ test), and their usability were evaluated in laboratory conditions. Macroscopically, pneumonia was seen in 120 of the lung tissue samples (11.73%). Based on the microscopic examinations, the pneumonia types were classified as catarrhal-suppurative bronchopneumonia (6.7%), fibrinous bronchopneumonia (5%), interstitial pneumonia (84.1%), and granulomatous pneumonia (4.2%). The IHC, IF and antigen ELISA …


Impact Of Light-Emitting Diode And Compact Fluorescent Lighting Type And Cage Tier On Layers Reared In An Enriched Cage System, Part 2: Some Welfare Traits, Gülsüm Tünaydin, Bi̇lgehan Yilmaz Di̇kmen Jan 2019

Impact Of Light-Emitting Diode And Compact Fluorescent Lighting Type And Cage Tier On Layers Reared In An Enriched Cage System, Part 2: Some Welfare Traits, Gülsüm Tünaydin, Bi̇lgehan Yilmaz Di̇kmen

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the effects of lighting type [light-emitting diode (LED) and compact fluorescent (FLO) light] and cage tier on welfare traits such as feather score, body and comb wounds, bumble foot and footpad dermatitis, beak damage, keel bone deformity, finger damage, aggressive pecking behavior, and the avoidance distance test (ADT) in laying hens reared in an enriched cage system. A total of 400 layers were used to determine these traits at 25 and 45 weeks of age. A total of 80 layers were used to determine tonic immobility (TI) duration and induction number. The …


The Impact Of Breeder Age On Egg Quality And Lysozyme Activity, Dorota Banaszewska, Barbara Biesiada-Drzazga, Dominik Ostrowski, Kamil Drabik, Justyna Batkowska Jan 2019

The Impact Of Breeder Age On Egg Quality And Lysozyme Activity, Dorota Banaszewska, Barbara Biesiada-Drzazga, Dominik Ostrowski, Kamil Drabik, Justyna Batkowska

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to analyze the biological components of eggs as well as lysozyme activity in relation to the age of hens. The research material consisted of eggs from parent stock of Ross 308 meat chickens. The eggs were evaluated at peak laying, i.e. eggs from 30-week-old hens, and after peak laying, i.e. from hens aged 60 weeks. Ninety eggs were evaluated in each period (180 eggs in total). Egg quality traits were divided into destructive and nondestructive traits. In addition, lysozyme activity in the albumen of the eggs was assessed. The quantitative characteristics of the eggs …


Ethnoveterinary Medicine In Turkey: A Comprehensive Review, Zeynep Büşra Erarslan, Şükran Kültür Jan 2019

Ethnoveterinary Medicine In Turkey: A Comprehensive Review, Zeynep Büşra Erarslan, Şükran Kültür

Turkish Journal of Veterinary & Animal Sciences

Today, traditional societies throughout the world possess a wealth of ethnoveterinary knowledge, which they have accumulated during prolonged interaction with nature. Turkey has ample resources of ethnoveterinary medicines as evident from its rich floral diversity and traditional botanical knowledge from the past. This review aims to compile herbal remedies used in ethnoveterinary medicine in Turkey with comprehensive usage information and to compare our findings with pharmacological studies. The botanical, family, and local names; used parts; preparation methods; administration/dosage and duration of the treatment; and ailments treated/therapeutic effects were presented by screening ethnobotanical and ethnoveterinary studies. The most cited plant families, …


College For Kids' 2018 Summer Camp: Improving Perceptions Of Science And Shaping Lifestyle Choices, Samuli M.O. Rauhalammi, Jamie C. Wolgast, Elissa F. Craig, Theresa M. Radke Jan 2019

College For Kids' 2018 Summer Camp: Improving Perceptions Of Science And Shaping Lifestyle Choices, Samuli M.O. Rauhalammi, Jamie C. Wolgast, Elissa F. Craig, Theresa M. Radke

ACA National Camp Research Forum Abstracts

No abstract provided.


Boiling Down Boilerplate In M&A Agreements: A Response To Choi, Gulati, & Scott, Robert Anderson, Jeffrey Manns Jan 2019

Boiling Down Boilerplate In M&A Agreements: A Response To Choi, Gulati, & Scott, Robert Anderson, Jeffrey Manns

GW Law Faculty Publications & Other Works

No abstract provided.


Tax Experimentation, Michael B. Abramowicz Jan 2019

Tax Experimentation, Michael B. Abramowicz

GW Law Faculty Publications & Other Works

Random experiments could allow the government to test tax policies before enactment into general law. Such experiments can be revenue-neutral, with the tax authority ensuring ex post that average tax revenues received from taxpayers in the treatment and control groups are equal. Taxpayers might thus volunteer even for experiments that would broaden the tax base, for example by eliminating deductions. Continued participation by taxpayers in such experiments would indicate that the proposed reforms are efficient at least if externalities are disregarded. Non-revenue-neutral experiments raise greater concerns about horizontal inequity, but may be helpful in addressing questions about effects of tax …


The Experience And Perception Of Physiotherapists In Nigeria Re: Fall Prevention In Recurrent-Faller Older Adults, Perpetua Chinenye Obi, Henrietha Chibuzor Nwankwo, Diaemeta Emofe, Isreal Adandom, Michael E. Kalu Jan 2019

The Experience And Perception Of Physiotherapists In Nigeria Re: Fall Prevention In Recurrent-Faller Older Adults, Perpetua Chinenye Obi, Henrietha Chibuzor Nwankwo, Diaemeta Emofe, Isreal Adandom, Michael E. Kalu

Internet Journal of Allied Health Sciences and Practice

Background: Effective fall prevention practices are essential for reducing falls among older adults. Rehabilitation professionals like physiotherapists are essential members of the fall prevention team, yet little is known about the experiences of physiotherapists practicing fall prevention in developing nations. Objective: To explore the experiences of physiotherapists in Nigeria who practice fall prevention among older adults. Method: We adopted a phenomenological approach to the traditional qualitative design in this study. We purposefully selected and conducted face-to-face interview with twelve physiotherapists who have treated at least one older adult who reported falling two or three times within last six months. Data …


Vol. 24: No. 2 (Spring 2019) Masthead Jan 2019

Vol. 24: No. 2 (Spring 2019) Masthead

Roger Williams University Law Review

No abstract provided.


Graphene Nanoflake Uptake Mediated By Scavenger Receptors, Fatima Alnasser, Valentina Castagnola, Luca Boselli, Margarita Esquivel-Gaon, Esen Efeoglu, Jennifer Mcintyre, Hugh Byrne, Kenneth A. Dawson Jan 2019

Graphene Nanoflake Uptake Mediated By Scavenger Receptors, Fatima Alnasser, Valentina Castagnola, Luca Boselli, Margarita Esquivel-Gaon, Esen Efeoglu, Jennifer Mcintyre, Hugh Byrne, Kenneth A. Dawson

Articles

The biological interactions of graphene have been extensively investigated over the last 10 years. However, very little is known about graphene interactions with the cell surface and how the graphene internalization process is driven and mediated by specific recognition sites at the interface with the cell. In this work, we propose a methodology to investigate direct molecular correlations between the biomolecular corona of graphene and specific cell receptors, showing that key protein recognition motifs, presented on the nanomaterial surface, can engage selectively with specific cell receptors. We consider the case of apolipoprotein A-I, found to be very abundant in the …


Vibrational Characterization Of Granulosa Cells From Patients Affected By Unilaterial Ovarian Endometriosis: New Insights From Infrared And Raman Microspectroscopy, Valentina Notarstefano, Giorgia Gioacchini, Hugh Byrne, Carlotta Zaca, Eleni Sereni, Lisa Vaccari, Andrea Borini, Oliana Carnevali Jan 2019

Vibrational Characterization Of Granulosa Cells From Patients Affected By Unilaterial Ovarian Endometriosis: New Insights From Infrared And Raman Microspectroscopy, Valentina Notarstefano, Giorgia Gioacchini, Hugh Byrne, Carlotta Zaca, Eleni Sereni, Lisa Vaccari, Andrea Borini, Oliana Carnevali

Articles

Endometriosis is a chronic gynaecological disease characterised by the presence of endometrial cells in extra-uterine regions. One of the main factors impacting on the fertility of women affected by endometriosis is the poor oocyte quality. Granulosa Cells (GCs) regulate oocyte development and maintain the appropriate microenvironment for the acquisition of its competence; hence, the dysregulation of these functions in GCs can lead to severe cellular damages also in oocytes. In this study, luteinized GCs samples were separately collected from both ovaries of women affected by Unilateral Ovarian Endometriosis and analysed by infrared and Raman microspectroscopy. The spectral data were compared …


Analysis Of Bodily Fluids Using Vibrational Spectroscopy: A Direct Comparison Of Raman Scattering And Infrared Absorption Techniques For The Case Of Glucose In Blood Serum, Drishya Prachalil, Clément Bruno, Franck Bonnier, Helen Blasco, Igor Chourpa, Matthew Baker, Jennifer Mcintyre, Hugh Byrne Jan 2019

Analysis Of Bodily Fluids Using Vibrational Spectroscopy: A Direct Comparison Of Raman Scattering And Infrared Absorption Techniques For The Case Of Glucose In Blood Serum, Drishya Prachalil, Clément Bruno, Franck Bonnier, Helen Blasco, Igor Chourpa, Matthew Baker, Jennifer Mcintyre, Hugh Byrne

Articles

Analysis of biomarkers present in the blood stream can potentially deliver crucial information on patient health and indicate the presence of numerous pathologies. The potential of vibrational spectroscopic analysis of human serum for diagnostic purposes has been widely investigated and, in recent times, infrared absorption spectroscopy, coupled with ultra-filtration and multivariate analysis techniques, has attracted increasing attention, both clinical and commercial. However, such methods commonly employ a drying step, which may hinder the clinical work flow and thus hamper their clinical deployment. As an alternative, this study explores the use of Raman spectroscopy, similarly coupled with ultra-filtration and multivariate analysis …


Raman Spectroscopy As A Potential Tool For Label Free Therapeutic Drug Monitoring In Human Serum: The Case Of Busulfan And Methotrexate, Drishya Parachalil, Franck Bonnier, Deirdre Commerford, Igor Chourpa, Jennifer Mcintyre, Hugh Byrne Jan 2019

Raman Spectroscopy As A Potential Tool For Label Free Therapeutic Drug Monitoring In Human Serum: The Case Of Busulfan And Methotrexate, Drishya Parachalil, Franck Bonnier, Deirdre Commerford, Igor Chourpa, Jennifer Mcintyre, Hugh Byrne

Articles

A methodology is proposed, based on Raman spectroscopy coupled with multivariate analysis, to determine the Limit of Detection (LOD) and Limit of Quantification (LOQ) for therapeutic drug monitoring in human serum, using the examples of Busulfan, a cell cycle non-specific alkylating antineoplastic agent, and, Methotrexate, a chemotherapeutic agent and immune system suppressant. In this study, ultrafiltration is employed to fractionate spiked human pooled serum to efficiently recover the drug in the filtrate prior to performing Raman analysis. The drug concentration ranges were chosen to encompass the recommended therapeutic ranges and toxic levels in patients. Raman spectra were collected from the …


Improved Performance Of Near Infrared Excitation Raman Spectroscopy Using Reflective Thin-Film Gold On Glass Substrates For Cytology Samples, Sinead Barton, Kevin O'Dwyer, Marion Butler, Hugh Byrne, Luke O'Neill, Bryan M. Hennelly Jan 2019

Improved Performance Of Near Infrared Excitation Raman Spectroscopy Using Reflective Thin-Film Gold On Glass Substrates For Cytology Samples, Sinead Barton, Kevin O'Dwyer, Marion Butler, Hugh Byrne, Luke O'Neill, Bryan M. Hennelly

Articles

Confocal near-infrared Raman spectroscopy has been shown to have applications in the area of clinical biology. A source wavelength in the near infrared is preferred over visible wavelengths for inspecting biological samples due to superior wave number resolution and reduced photo damage. However, these excitation sources have a number of drawbacks when compared to lasers in the visible wavelength region, including the requirement to use expensive highly pure crystal substrates such as Raman grade calcium fluoride as well as long acquisition times due to the lower Raman scattering efficiency. This paper investigates the use of a reflective substrate comprising a …


Numerically Modelling Time And Dose Dependent Cytotoxicity, Hugh Byrne, Marcus A. Maher Jan 2019

Numerically Modelling Time And Dose Dependent Cytotoxicity, Hugh Byrne, Marcus A. Maher

Articles

Dose-response curves are fundamental tools of in vitro toxicology, extensively employed in toxicant or drug screening. They are expressed by a variety of end-points and assays, measured at different time-points in a choice of cell-lines, but are typically quantified only using the mean concentration for 50% response (e.g. drug efficacy or pathway inhibition) as an indicator of overall effect. However, the response is the result of a complex and dynamic cascade of events which occur between the initial exposure and the measured end-point, and the characteristic rates of the contributing stages govern the dose response and ultimately the measured characteristic …