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Identification Of Novel Adenylyl Cyclase 5 (Ac5) Signaling Networks In D1 And D2 Medium Spiny Neurons Using Bimolecular Fluorescence Complementation Screening, Trevor B. Doyle, Brian S. Muntean, Karin F. Ejendal, Michael P. Hayes, Monica Soto-Velasquez, Kirill A. Martemyanov, Carmen W. Dessauer, Chang-Deng Hu, Val J. Watts

Purdue University Libraries Open Access Publishing Fund

Adenylyl cyclase type 5 (AC5), as the principal isoform expressed in striatal medium spiny neurons (MSNs), is essential for the integration of both stimulatory and inhibitory midbrain signals that initiate from dopaminergic G protein-coupled receptor (GPCR) activation. The spatial and temporal control of cAMP signaling is dependent upon the composition of local regulatory protein networks. However, there is little understanding of how adenylyl cyclase protein interaction networks adapt to the multifarious pressures of integrating acute versus chronic and inhibitory vs. stimulatory receptor signaling in striatal MSNs. Here, we presented the development of a novel bimolecular fluorescence complementation (BiFC)-based protein-protein interaction …

Role Of Probiotics In Non-Alcoholic Fatty Liver Disease: Does Gut Microbiota Matter?, Chencheng Xie, Dina Halegoua-De Marzio

Division of Gastroenterology and Hepatology Faculty Papers

Non-alcoholic fatty liver disease (NAFLD) is the hepatic consequence of metabolic syndrome, which often also includes obesity, diabetes, and dyslipidemia. The connection between gut microbiota (GM) and NAFLD has attracted significant attention in recent years. Data has shown that GM affects hepatic lipid metabolism and influences the balance between pro/anti-inflammatory effectors in the liver. Although studies reveal the association between GM dysbiosis and NAFLD, decoding the mechanisms of gut dysbiosis resulting in NAFLD remains challenging. The potential pathophysiology that links GM dysbiosis to NAFLD can be summarized as: (1) disrupting the balance between energy harvest and expenditure, (2) promoting hepatic …

Keeping Allergen Names Clear And Defined, Sanny K. Chan, Anna Pomés, Christiane Hilger, Janet M. Davies, Geoffrey Mueller, Annette Kuehn, Andreas L. Lopata, Gabriele Gadermaier, Marianne Van Hage, Monika Raulf, Richard E. Goodman

Department of Food Science and Technology: Faculty Publications

The World Health Organization/International Union of Immunological Societies (WHO/IUIS) Allergen Nomenclature Sub-Committee was established in 1986 by leading allergists to standardize names given to proteins that cause IgE-mediated reactions in humans. The Sub-Committee’s objective is to assign unique names to allergens based on a critical analysis of confidentially submitted biochemical and clinical data from researchers, often prior to publication to preserve consistency. The Sub-Committee maintains and revises the database as the understanding of allergens evolves. This report summarizes recent developments that led to updates in classification of cockroach group 1 and 5 allergens to animal as well as environmental and …

Open Access: Could Defeat Be Snatched From The Jaws Of Victory?, Richard Poynder

Copyright, Fair Use, Scholarly Communication, etc.

When news broke early in 2019 that the University of California had walked away from licensing negotiations with the world’s largest scholarly publisher (Elsevier), a wave of triumphalism spread through the OA Twittersphere. The talks had collapsed because of Elsevier’s failure to offer UC what it demanded: a new-style Big Deal in which the university got access to all of Elsevier’s paywalled content plus OA publishing rights for all UC authors – what UC refers to as a “Read and Publish” agreement. In addition, UC wanted Elsevier to provide this at a reduced cost.1 Given its size and influence, UC’s …

The Impact Of Systemic Precision Medicine And Immunotherapy Treatments On Brain Metastases, Rowland H. Han, Gavin P. Dunn, Milan G. Chheda, Albert H. Kim

Open Access Publications

Metastases from melanoma, lung and breast cancer are among the most common causes of intracranial malignancy. Standard of care for brain metastases include a combination of surgical resection, stereotactic radiosurgery, and whole-brain radiation. However, evidence continues to accumulate regarding the efficacy of molecularly-targeted systemic treatments and immunotherapy. For non-small cell lung cancer (NSCLC), numerous clinical trials have demonstrated intracranial activity for inhibitors of EGFR and ALK. Patients with melanoma brain metastases may benefit from systemic therapy using BRAF-inhibitors with and without trametinib. Several targeted options are available for breast cancer brain metastases that overexpress HER2, although agents with intracranial activity …

Investigating The Origins Of Toxins Present In Electronic Cigarette Aerosols, Shawna Vreeke

Dissertations and Theses

The purported safety of electronic cigarettes has come under scrutiny with the significant increase of lung related illnesses starting in the summer of 2019. Public view has started to shift towards understanding the potential negative health impact associated with these devices. While many investigations indicate probable hazards present in e-cigarette aerosols, inter-laboratory assessments are wide ranging and can be contradictory. Due to the novelty of this field, relatively little is known about these products. In this work, the identification and quantification of inhalation toxicants such as formaldehyde, acrolein, acetaldehyde and dihydroxyacetone are reported. Results of the investigation of the ability …

Defining The Layers Of A Sensory Cilium With Storm And Cryoelectron Nanoscopy, Michael A Robichaux, Valencia L Potter, Zhixian Zhang, Feng He, Jun Liu, Michael F Schmid, Theodore G Wensel

Faculty, Staff and Students Publications

Primary cilia carry out numerous signaling and sensory functions, and defects in them, "ciliopathies," cause a range of symptoms, including blindness. Understanding of their nanometer-scale ciliary substructures and their disruptions in ciliopathies has been hindered by limitations of conventional microscopic techniques. We have combined cryoelectron tomography, enhanced by subtomogram averaging, with superresolution stochastic optical reconstruction microscopy (STORM) to define subdomains within the light-sensing rod sensory cilium of mouse retinas and reveal previously unknown substructures formed by resident proteins. Domains are demarcated by structural features such as the axoneme and its connections to the ciliary membrane, and are correlated with molecular …

Here Now And Later There, Dartanyan Ball

The Anthology

No abstract provided.

Clinical Implications In Vaginal Orgasm Response, Lindsey M. Brown Mccormick, Sherry Todd, Laura Schmuldt, Kathryn Russ, Cristen Wathen

Journal of Counseling Sexology & Sexual Wellness: Research, Practice, and Education

Previous research has shown that counselors feel uncomfortable addressing clients’ sexual concerns due to a lack of education on topics related to human sexuality. Various studies have attempted to identify the characteristics of vaginal orgasm, including whether women and other people with vaginas (PWV) can achieve different kinds of orgasms. The current study examines responses to participants surveyed across the United States on their orgasm response and compares responses of participants who achieved orgasm through masturbation and those who achieved orgasm through sex with a partner to determine whether PWV experience one kind of orgasm during masturbation and experience a …

The Ticker, November 18, 2019

The Ticker

The Ticker is the student newspaper of Baruch College. It has been published continuously since 1932, when the Baruch College campus was the School of Business and Civic Administration of the City College of New York.

Monitoring The Potential Fish Aggregating Device Effect Of The Ocean Cleanup's Barrier In The Great Pacific Garbage Patch, Briana Rose Gibbs

Open Access Theses

Plastic pollution is a persistent transboundary problem in the open ocean. The largest accumulation zone of pelagic plastics is the Great Pacific Garbage Patch (GPGP), located in the North Pacific Subtropical Gyre. The Ocean Cleanup is a non-profit dedicated to removing pelagic plastics using a barrier system designed to passively collect plastic in the GPGP. This study presents acoustic monitoring of the biological impact of The Ocean Cleanup’s barrier system during its trial in the GPGP between 22 October and 29 December 2018. The acoustic monitoring was conducted using commercially available echosounders to monitor fish biomass presence and absence. It …

Satellite Infrared Retrievals Of Sea Surface Temperature At High Latitudes, Chong Jia

Open Access Theses

Climate change is amplified in the Arctic region (north of 60 ° N) relative to elsewhere. By analyzing climate model simulations, it has been found that the largest contribution to Arctic amplification comes from temperature feedbacks, due to both the different warming profile in low and high latitudes and a larger temperature increase in longwave emission per unit of warming at colder background temperatures compared to tropical conditions. Satellite remote sensing offers the best way of deriving sea surface temperature (SST) in the Arctic, but given that the retrieval algorithms in the infrared (IR) are designed to compensate for the …

Ada News - 11/18/2019, American Dental Association, Publishing Division

ADA News

Established in 1970 as the official newspaper of the American Dental Association, the ADA News serves practicing dentists and others allied to the dental profession in the U.S. and internationally. It is the No. 1 source of news and information about the many benefits and services the ADA delivers to members daily as well as timely information on scientific, social, political and economic developments affecting dentistry and health care.

Student Engages With Hands-On Learning In Genetic Testing, Allison Brace

College of Arts and Sciences Newsroom

Katie Parker has spent most of her four years at the University of Dayton searching for a chemical to minimize the effects of brain cancer.

Senate Executive Committee Minutes November 18, 2019, Bronx Community College Senate Executive Committee

BCC Governance Archives

Minutes for the meeting of the Senate Executive Committee on November 18, 2019.

The Hydrogen Bond: A Hundred Years And Counting, Steve Scheiner

Chemistry and Biochemistry Faculty Publications

Since its original inception, a great deal has been learned about the nature, properties, and applications of the H-bond. This review summarizes some of the unexpected paths that inquiry into this phenomenon has taken researchers. The transfer of the bridging proton from one molecule to another can occur not only in the ground electronic state, but in various excited states as well. Study of the latter process has developed insights into the relationships between the nature of the state, the strength of the H-bond, and the height of the transfer barrier. The enormous broadening of the range of atoms that …

The Daily Gamecock, Monday, November 18, 2019, University Of South Carolina, Office Of Student Media

November

No abstract provided.

Designation Of A Neotype For Oeneis Melissa (Fabricius, 1775) And A Lectotype For Oeneis Polixenes (Fabricius, 1775) (Lepidoptera: Nymphalidae: Satyrinae), Crispin S. Guppy

The Taxonomic Report of the International Lepidoptera Survey

The Oeneis melissa (Fabricius, 1775) “neotype” designations of Lukhtanov and Eitschberger (2000; 2001) do not meet several critical requirements of the ICZN and are therefore invalid. In addition, the locality data is too vague to be taxonomically useful, their “neotype” specimen is a specimen of the taxon generally known as O. melissa semplei, and acceptance of their specimen as neotype would change the meaning of the taxon Oeneis melissa. Hence, I reject their neotype designation. A neotype Oeneis melissa (Fabricius, 1775) is designated from Nain, Labrador with careful consideration of the Articles of the International Code of Zoological …

Sea Robin, Prionotus Carolinus , Neurocranium, Alexandra L. Jackson, Mia I. Andahazy, David Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

No abstract provided.

Gut Microbiota: From The Forgotten Organ To A Potential Key Player In The Pathology Of Alzheimer's Disease, Dong-Oh Seo, David M Holtzman

Open Access Publications

More than 300 years ago, Antony van Leewenhoeck first described observing single-celled microorganisms, which he termed "animalcules," examining his saliva under a microscope. Although the idea of the coexistence of microorganisms in our body is not new, we have only recently been able to investigate their ecological relationship to our body, with the development of high-throughput molecular techniques. The diverse microorganism communities residing in our guts are established and maintained by complex interactions among microorganisms and their host. Notably, their alteration has been implicated in influencing various diseases including neurological diseases. Alzheimer's disease (AD) is the most common cause of …

My Library, My Story: Walt, Walter Cressler

University Libraries Personnel and Library History

No abstract provided.

Salt Restriction Lowers Blood Pressure At Rest And During Exercise Without Altering Peripheral Hemodynamics In Hypertensive Individuals, Stephen M. Ratchford, Ryan M. Broxterman, Taylor La Salle, Oh-Sung Kwon, Song-Young Park, Paul N. Hopkins, Russell S. Richardson, Joel D. Trinity

Health and Kinesiology Faculty Publications

Dietary salt restriction is a well-established approach to lower blood pressure and reduce cardiovascular disease risk in hypertensive individuals. However, little is currently known regarding the effects of salt restriction on central and peripheral hemodynamic responses to exercise in those with hypertension. Therefore, this study sought to determine the impact of salt restriction on the central and peripheral hemodynamic responses to static-intermittent handgrip (HG) and dynamic single-leg knee extension (KE) exercise in individuals with hypertension. Twenty-two subjects (14 men and 8 women, 51 ± 10 yr, 173 ± 11 cm, 99 ± 23 kg) forewent their antihypertensive medication use for …

Sea Robin (Prionotus Carolinus) Neurocranium, Brittany A. Malin, Mykenzee L. Munaco, David W. Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

Neurocranium from 7.7cm total length (TL) Sea Robin (Prionotus carolinus), collected from a preserved mixed-fishes set from Carolina Biological Supply Company

Structural Analysis Of The Regulatory Gaf Domains Of Cgmp Phosphodiesterase Elucidates The Allosteric Communication Pathway, Richa Gupta, Yong Liu, Huanchen Wang, Christopher T. Nordyke, Ryan Z. Puterbaugh, Wenjin Cui, Krisztina Varga, Feixia Chu, Hengming Ke, Harish Vashisth, Rick H. Cote

Faculty Publications

Regulation of photoreceptor phosphodiesterase (PDE6) activity is responsible for the speed, sensitivity, and recovery of the photoresponse during visual signaling in vertebrate photoreceptor cells. It is hypothesized that the physiological differences in the light responsiveness of rods and cones may result in part from differences in the structure and regulation of the distinct isoforms of rod and cone PDE6. Although rod and cone PDE6 catalytic subunits share a similar domain organization consisting of tandem GAF domains (GAFa and GAFb) and a catalytic domain, cone PDE6 is a homodimer whereas the rod PDE6 catalytic dimer is composed of two homologous catalytic …

The Impact Of Msaabcr On Sara-Associated Phenotypes Is Different In Divergent Clinical Isolates Of Staphylococcus Aureus, Joseph S. Rom, Aura M. Ramirez, Karen E. Beenken, Gyan S. Sahukhal, Mohamed O. Elasri, Mark S. Smeltzer

Faculty Publications

The staphylococcal accessory regulator (sarA) plays an important role in Staphylococcus aureus infections including osteomyelitis, and the msaABCR operon has been implicated as an important factor in modulating expression of sarA. Thus, we investigated the contribution of msaABCR to sarA-associated phenotypes in the S. aureus clinical isolates LAC and UAMS-1. Mutation of msaABCR resulted in reduced production of SarA and a reduced capacity to form a biofilm in both strains. Biofilm formation was enhanced in a LAC msa mutant by restoring the production of SarA, but this was not true in a UAMS-1 msa mutant. Similarly, …

The Free Press Vol 51, Issue 9, 11-18-2019, Alyson Peabody

Free Press, The

Student researches pollution in Casco Bay--Legal action taken against Cape Elizabeth high school--Inedible items found in food at Brooks dining hall--Maine graduates may qualify for tax credit

Points Of Pride, Georgia Southern University

Points of Pride (2013-2023)

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Neuron Specific Gene 2 (Nsg2): A Novel Regulator Of Excitatory Neurotransmission Via Ampa Receptor Trafficking At A Subset Of Glutamatergic Synapses, Praveen Chander

Biomedical Sciences ETDs

Members of the Neuron-Specific Gene family (NSG1-3) play critical roles in excitatory synaptic transmission via regulation of AMPA receptor surface expression within the post-synaptic density (PSD). While NSG1 and NSG3 regulate AMPAR recycling and endocytosis, respectively, the function of NSG2 has remained elusive. Here we undertook a series of studies to assess the role of NSG2 in excitatory synaptic transmission. We found that a portion of NSG2 punctae localized with HOMER1 and surface AMPARs at excitatory synapses and that NSG2 AMPAR subunits in mouse brain. Knockout of NSG2 selectively impaired the frequency of AMPA mEPSCs, while overexpression caused a significant …

Water‐Borne And Plasma Corticosterone Are Not Correlated In Spotted Salamanders, Alice R. Millikin, Sarah K. Woodley, Drew R. Davis, Ignacio T. Moore, James T. Anderson

School of Earth, Environmental, & Marine Sciences Faculty Publications

Water‐borne hormone measurement is a noninvasive method suitable for amphibians of all sizes that are otherwise difficult to sample. For this method, containment‐water is assayed for hormones released by the animal. Originally developed in fish, the method has expanded to amphibians, but requires additional species‐specific validations. We wanted to determine physiological relevance of water‐borne corticosterone in spotted salamanders (Ambystoma maculatum) by comparing concentrations to those taken using established corticosterone sampling methods, such as plasma. Using a mixture of field and laboratory studies, we compared water‐borne corticosterone levels to other traditional methods of sampling corticosterone for spotted salamander larvae, metamorphs, and …

Thermal Stress Responses Of Sodalis Glossinidius, An Indigenous Bacterial Symbiont Of Hematophagous Tsetse Flies, Jose Santinni Roma, Shaina D’Souza, Patrick J. Somers, Leah F. Cabo, Ruhan Farsin, Serap Aksoy, Laura J. Runyen-Janecky, Brian L. Weiss

Biology Faculty Publications

Tsetse flies (Diptera: Glossinidae) house a taxonomically diverse microbiota that includes environmentally acquired bacteria, maternally transmitted symbiotic bacteria, and pathogenic African trypanosomes. Sodalis glossinidius, which is a facultative symbiont that resides intra and extracellularly within multiple tsetse tissues, has been implicated as a mediator of trypanosome infection establishment in the fly’s gut. Tsetse’s gut-associated population of Sodalis are subjected to marked temperature fluctuations each time their ectothermic fly host imbibes vertebrate blood. The molecular mechanisms that Sodalis employs to deal with this heat stress are unknown. In this study, we examined the thermal tolerance and heat shock response of …