Digital Commons Network^™

Effects And Mechanisms Of Anacardium Occidentale And Glochidion Zeylanicum Leaf Extracts On Neuroprotective, Neuritogenesis, Oxidative Stress Resistance And Anti-Aging Properties., Chatrawee Duangjan

Chulalongkorn University Theses and Dissertations (Chula ETD)

Aging is the primary risk factor for most neurodegenerative diseases and can negatively affect the quality of life. Neurodegenerative diseases are linked to neuronal cell death and neurite outgrowth impairment that are often caused by oxidative stress. As people want to live longer and healthier, healthy nutrition has been increasingly received much attention in recent years. Several studies reported a positive correlation between antioxidants in foods and longevity. Natural products from food supplements and medicinal plants with antioxidant properties could be promising candidates for fighting against various aging-related diseases and promoting longevity. Leaf extracts from A. occidentale (AO) and G. …

Citation Network Analysis For Journal Selection Of University Library, Nopanan Pongitthidej

Chulalongkorn University Theses and Dissertations (Chula ETD)

Every academic year, a university library must subscribe tons of journals to provide the best resource for the students and researchers. However, subscribed journals have been used unequally and some relevant journals are not subscribed. Therefore, the library needs supporting information about usedness and demand of journals for efficient collection management. In this paper, citation network analysis is applied to analyze the articles published by university researchers and their references. The result is two overlapping lists of journals that are recommended for the library collection i.e. (a) journals that the university researchers have published in and (b) journals that the …

Access To Higher Education, Job Mismatch, And Wage Penalties In Thailand, Palita Saripan

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study considers whether close proximity to higher education institutions increases the probability of earning a bachelor’s degree, overeducation, and horizontal mismatch between degree and occupation. Using data from the 2015 and 2016 Labor Force Survey of the National Statistical Office of Thailand, and jobs data from the Ministry of Labor, we find that individuals living in provinces with research or Rajabhat universities are more likely to earn a bachelor’s degree, but does not increase the probability of being overeducated for their job. In addition, we find larger overeducation penalties in provinces with universities. Finally, qualitative analysis shows that demand …

Characterization Of Dissolved Organic Matter In Treated Textile Wastewater From Membrane Bioreactor, Nichapach Puangmalai

Chulalongkorn University Theses and Dissertations (Chula ETD)

This research aims to investigate the DOM removal efficiency in textile wastewater by MBR at different sludge retention time. In addition, the characteristics of DOM was investigated by using resin fractionation and fluorescent excitation-emission matrix techniques. Textile wastewater was synthesized by using commercial dyes and used in all experiment. The characteristics of synthesis textile wastewater showed that the COD concentration was high as 2,000 mg/L. In addition, the DOC concentration in synthesis textile wastewater was also high at 466.1 mg/L. MBR was conducted under HRT 2.5 days and SRT was varied at 15 days, 30 days and infinite, respectively. MBR …

Analyzing And Forecasting Online Tour Bookings Using Google Analytics Metrics, Jiaranai Awichanirost

Chulalongkorn University Theses and Dissertations (Chula ETD)

In today's world, the internet is growing fast and has revolutionized many business operations in the tourism industry. The tourism industry plays an important role in Thailand's GDP and is a great boost to the domestic economy. Most tour operators have created websites to be used as part of their business operations which are the main way to build relationships with customers and sales and hence make the website performance measurement an important strategic factor for online marketing. The objective of this research is to analyze the significant impact of Google Analytics metrics that is a measurement of website performance …

Functional And Transcriptomic Changes In Synergy Of Endotoxin And (1->3)-Β-D-Glucan In Bone Marrow Derived Macrophage From Fc Gamma Receptor Iib Deficient Mice, Jiraphorn Issara-Amphorn

Chulalongkorn University Theses and Dissertations (Chula ETD)

Systemic lupus erythematosus (SLE) is an autoimmune disease with a diverse array of clinical symptoms. There are many factors including environmental factors may trigger disease progression. In this study, we investigated the influence of spontaneous gut leakage upon polymicrobial sepsis in Fc gamma receptor IIb deficient (FcgRIIb-/-) mice aged 8 and 40 weeks, as representing asymptomatic and symptomatic lupus, respectively. The spontaneous gut leakage as determined by i) the level of serum FITC-dextran, ii) increased serum endotoxin (LPS), and iii) increased serum (1->3)-b-D-glucan (BG), were demonstrated in symptomatic FcgRIIb-/- mice, but not in asymptomatic group. Moreover, spontaneous gut leakage …

Effects Of Non-Albicans Candida Species On Biofilm Formation And Acidogenicity Of Dual-Species Biofilms And On Cariogenic Virulence Gene Expression Of Streptococcus Mutans, Wirunphat Sansanoa

Chulalongkorn University Theses and Dissertations (Chula ETD)

Early childhood caries is a common oral disease found in children which could affect their general health. Streptococcus mutans is an important cariogenic pathogen since it possesses cariogenic properties, which are acid production, acid tolerance and biofilm formation. Recent studies showed that Candida albicans, which is frequently found together with S. mutans in plaques of children with dental caries, enhances cariogenicity. At the same time, increasing prevalence of non-albicans Candida species (NACs) in children and immune-compromised patients has been reported. Therefore, this study aims to investigate the effects of NACs when co-cultured with S. mutans on cariogenic properties and virulence …

Utilization Of Modified Mrna Encoding Bone Morphogenetic Protein-2 For Periodontal Regeneration: An In Vitro Study, Promphakkon Kulthanaamondhita

Chulalongkorn University Theses and Dissertations (Chula ETD)

Current modalities for periodontal regeneration provide modest success, however complete periodontal regeneration is still not achievable. Recently in vitro synthesized nucleoside-modified messenger RNA (mRNA) has emerged as a novel platform in regenerative medicine. The aims of this study are to investigate the ability of human periodontal ligament cells (PDLCs), clinically relevant target cells, to produce bone morphogenetic protein-2 (BMP-2), a significant protein for bone formation after transfected with modified mRNA that encode this protein. We investigated the ability of translated protein for enhancing PDLC proliferation and promotes endothelial cell tube formation, marker of angiogenesis. Isolated PDLCs from healthy periodontal tissue …

Genome, Physicochemical Properties And Esterase Gene Cloning Of Novel Lentibacillus And Halobacillus Species, Auttaporn Booncharoen

Chulalongkorn University Theses and Dissertations (Chula ETD)

A total of 831 moderately halophilic bacteria isolated from various samples in Thailand were screened for their lipolytic activity. Three hundred and forty-nine isolates showed activity on agar plate while 322 isolates exhibited in broth. The grouping and identification of 322 isolates based on DNA-fingerprinting using (GTG)5+ERIC2 primer and 16S rRNA gene sequence, 180 DNA patterns could be generated. The result indicated that the representative isolates belonged to genera Bacillus, Halobacillus, Lentibacillus, Marinobacter, Thallossobacillus, and Virgibacillus. On the basis of polyphasic taxonomic study and genome analysis, a selected moderately halophilic strain SSKP1-9T, Gram-staining-positive, aerobic, endospore-forming, rod-shaped was closely related to …

In Vitro Antiplatelet Activity Of Lusianthridin In Human Platelets, Hla Nu Swe

Chulalongkorn University Theses and Dissertations (Chula ETD)

Lusianthridin is a phenanthrene derivative isolated from Dendrobium venustum. Some phenanthrene compounds have antiplatelet aggregation activities via undefined pathways. The objective of this study is to determine the inhibitory effects and potential mechanisms of lusianthridin on platelet aggregation. Turbidometric aggregometry method was used to measure the extent of platelet aggregation, then cyclic adenosine monophosphate (cAMP) levels were measured by ELISA kit. Inhibitory effect of lusianthridin on cyclooxygenase enzymes was analyzed by COX fluorescent inhibitor screening assay kit. The results indicated that Lusianthridin most potently inhibited arachidonic acid -induced platelet aggregation by maximum percent inhibition of aggregation of 89.7 ± 1.2 …

Cytotoxic Constituents Of Cymbidium Finlaysonianum And Paphiopedilum Dianthum, Nonthalert Lertnitikul

Chulalongkorn University Theses and Dissertations (Chula ETD)

Chemical investigation of two orchids native to Thailand led to the isolation of eighteen compounds. From the whole plants of Cymbidium finlaysonianum Wall. ex Lindl, a new dihydrophenanthrene, 1-(4-hydroxybenzyl)-2,7-dihydroxy-4,6-dimethoxy-9,10-dihydrophenan-threne, and nine known stilbenoids, including a phenanthrenequinone, cymbinodin-A, a dihydrophenanthrenequinone, ephemeranthoquinone B, a bibenzyl, batatasin III, two phenanthrenes, 2,4-dimethoxy-3,7-dihydroxyphenanthrene and 3,7-dihydroxy-2,4,6-trimethoxyphenanthrene, and four dihydrophenanthrenes i.e. coelonin, 6-methoxycoelonin, flavanthridin and lusianthridin, were obtained. From the roots and leaves of Paphiopedilum dianthum Tang & Wang, two new stilbene dimers, paphiodianthins A and B, were isolated along with three known stilbenes i.e. pinosylvin monomethyl ether, 2,3'-dihydroxy-5'-methoxystilbene and (E)-2,5'-dihydroxy-2'-(4-hydroxybenzyl)-3'-methoxystilbene, and three known flavonoids i.e. isalpinin, …

Factors Students Consider In The Decision Making Process For Graduate Education, Kiley Sturm

Masters Theses

Using a qualitative methodology, the researcher studied the factors students consider in the decision making process for graduate education. Through conducting two focus groups that contained a total of thirteen participants, the researcher identified the factors that were the most significant to their decision making during the decision making process for pursuing graduate education, applying to institutions, and the selection and enrollment at their institution. The researcher also identified reasons affecting the students' decision of when to pursue graduate education and how their undergraduate experience impacted the decision making process.

Stability Of Temperate Coral Astrangia Poculata Microbiome Is Reflected Across Different Sequencing Methodologies, Dawn B. Goldsmith, Zoe A. Pratte, Koty H. Sharp

Arts & Sciences Faculty Publications

The microbiome of the temperate coral Astrangia poculata was first described in 2017 using next-generation Illumina sequencing to examine the coral’s bacterial and archaeal associates across seasons and among hosts of differing symbiotic status. To assess the impact of methodology on the detectable diversity of the coral’s microbiome, we obtained near full-length Sanger sequences from clone libraries constructed from a subset of the same A. poculata samples. Eight samples were analyzed: two sets of paired symbiotic (brown) and aposymbiotic (white) colonies collected in the fall (September) and two sets collected in the spring (April). Analysis of the Sanger sequences revealed …

Retinoic Acid Inhibition Impairs Planarian Eye Regeneration, Gabrielle Bennetti, Aaron Corbin-Leftwich, Linda M. Boland, Isaac Skromne

Biology Faculty Publications

Retinoic acid is a known morphogen in regulating animal growth and development. Planaria are a key model system for regeneration and their eyes are a morphological marker of anterior differentiation. We explored the requirement for retinoic acid signaling in the regeneration of body parts in the planaria S. mediterranea using an inhibitor of retinoic acid synthesis, diethylaminobenzaldehyde (DEAB). Whole planaria, soaked in DEAB for three days prior to and five days following amputation, produced trunk and tail fragments with defective anterior regeneration. Following regeneration, up to 80% of posterior fragments developed abnormal eyes. The abnormalities included animals without eyes, with …

Conformational Flexibility In The Enterovirus Rna Replication Platform, Meghan S. Warden, Kai Cai, Gabriel Cornilescu, Jordan E. Burke, Komala Ponniah, Samuel E. Butcher, Steven M. Pascal

Chemistry & Biochemistry Faculty Publications

A presumed RNA cloverleaf (5′CL), located at the 5′-most end of the noncoding region of the enterovirus genome, is the primary established site for initiation of genomic replication. Stem–loop B (SLB) and stem–loop D (SLD), the two largest stem–loops within the 5′CL, serve as recognition sites for protein interactions that are essential for replication. Here we present the solution structure of rhinovirus serotype 14 5′CL using a combination of nuclear magnetic resonance spectroscopy and small-angle X-ray scattering. In the absence of magnesium, the structure adopts an open, somewhat extended conformation. In the presence of magnesium, the structure compacts, bringing SLB …

Functional Characterization Of Glutamate Carboxypeptidase Ii In Caenorhabditis Elegans, Hashni Epa Vidana Gamage

Masters Theses

Glutamate carboxypeptidase II (GCPII) is a transmembrane zinc metalloprotease expressed in a number of organisms: from yeast to worm to humans. In humans, GCPII has been observed as a multifunctional protein and expressed in prostate, intestine, kidney, brain, tumor-associated neovasculature and other tissues as five paralogs. In the human small intestine, hGCPII is proposed to facilitate the folate absorption by cleaving terminal glutamate residues in dietary folates. Folates act as a cofactor in one-carbon metabolic pathways such as nucleotide synthesis, amino acid synthesis, DNA repair, and consequently involved in cell division and growth. The hGCPII homolog is found in the …

Exploratory Factor Analysis Of The Canadian Wechsler Intelligence Scale For Children-Fifth Edition For A Sample Of First Nations Students, Jessica Hanson

Masters Theses

Native Americans and First Nation students are overrepresented in special education and underrepresented in structural bias research of the intelligence measures that place them there. There are several empirical studies of test bias on the Wechsler scales due to their popularity within the school system, however there is little exploratory factor analysis research on these scales with the Native American Indian population. Further, the Native American Indian and First Nation population is a relatively small minority group compared to other racial and ethnic groups in North America and this group is underrepresented in government statistics and overlooked in funding for …

Variation In Leaf Morphology Of An Alpine Shrub Community Along Environmental Gradients, Abdulssalam Khafsha

Masters Theses

Mountains represent complex environment gradients which strongly affect local temperature regimes and rainfall. These changes in climate conditions drive a diversity of plant responses. As alpine environments are expected to change dramatically in response to climate change, understanding plant responses to environmental variation is critical in these environments. Although they are a critical component of alpine communities, the environmental responses of shrubs have been much less examined than trees, representing an important knowledge gap. Leaves, as the primary photosynthetic organs on plants, would be expected to respond strongly to local climate conditions. In this study, I documented how the leaf …

White Grub In Bluegill In The Sangamon River: Impact Of Sewage Effluent And Flow Regime On Infection Parameters, Miranda White

Masters Theses

White grub (Posthodiplostomum minimum) is a digenetic trematode that uses a piscivorous bird, a pulmonate snail, and a centrarchid fish to complete its life cycle. To assess the impact of impoundment and sewage effluent on white grub infection parameters, infections in bluegill (Lepomis macrochirus) were monitored in 3 reaches of the Sangamon River near Decatur, Illinois: Reach 1- free-flowing river upstream of Lake Decatur Dam, unimpacted by sewage effluent, Reach 2- impounded flow below Lake Decatur Dam, unimpacted by sewage effluent, and Reach 3- free-flowing river downstream of Sanitary District of Decatur, impacted by sewage effluent. Liver and kidney abundances …

Haplotype Diversity And Population Genetic Structure Of Antistrophus Gall Wasps Associated With Two Silphium Species And Implication For Host Mediated Speciation, Jiaxin Deng

Masters Theses

Gall making insects form a special feeding guild of phytophagous animals, and by manipulating host plant tissue differentiation, are able to avoid plant chemical defenses and thus have no need for counter defense mechanisms. Host plant selection is crucial to the evolution of these insects because successful gall formation is largely dependent on host plant ability to respond to stimuli. In Illinois and neighboring states, Antistrophus gall wasps associated with the rosin weed (S. integrifolium) and the cup plant (S. perfoliatum) are morphologically indistinguishable and thus have been treated as belonging to single species. However, the wasps from the host …

Role Of Emotion Regulation In The Relationship Between Fears Of Evaluation And Social Anxiety, Jaismeen Dua

Masters Theses

Cognitive components influencing social anxiety have been well-researched for decades, especially fear of negative evaluation (Clark & Wells, 1995; Hofmann, 2007; Rapee & Heimberg, 1997; Schlenker & Leary, 1982). Relatively recent and emerging research has suggested a strong link between fear of negative evaluation and fear of positive evaluation, and how both of them influence social anxiety (Weeks, Heimberg, & Rodebaugh, 2008; Weeks et al., 2007; Weeks & Howell, 2012; Weeks et al., 2009). This study examined social anxiety in relation to both fear of negative evaluation and fear of positive evaluation. Findings from the study replicated previous research results, …

Synthesis, Characterization And Mechanistic Studies Of Biomimetic Dinuclear Copper(Ii) Complexes, Millaniyage Nuwanthaka Prasad Jayaweera

Masters Theses

Dinuclear copper complexes are significant synthetic targets due to their use as model compounds for the active sites of type 3 copper proteins like catechol oxidase. A distinct class of ligands used in these studies are built on a phenol core to witch various donor side-arms were appended at the 2 and 6 positions of the arene ring. In our quest to quantify the influence of electron donating or withdrawing groups attached to the central arene ring, we have designed a set of ligands that, upon coordination to copper(II) centers, would produce complexes of the general structure. During this research …

Developing A Basket-Shaped Host Molecule Based On P-Tert-Butylcalix[4]Arene Featuring Urea Groups For Encapsulating Volatile Guest Molecules, Lokugama Widanelage Vidula Priyadarshana

Masters Theses

Molecular baskets are a group of synthetic host molecules which are designed to govern the inclusion of small guest molecules. The basic structural features of basket-shaped host molecules are a cavity for holding a much smaller guest molecule and tunable gates for keeping the guest molecule inside. These types of gates can be tuned by changing temperature, concentration, pH, etc. In this research group's previous study, a molecular basket of cal ix[ 4]arene with carboxylic acid groups was synthesized by functionalizing calix[4]arene's phenol hydroxyl groups with benzyl bromide building blocks. In the study detailed here, 1 , l '-((5-(bromomethyl)-1,3-phenylene)bis(methylene))bis(3-isopropylurea) was …

The Transcriptional Response Of Phanerochaete Chrysosporium And Trametes Versicolor To Growth On Stems Of Helianthus Argophyllus (Silverleaf Sunflower), Nadh Hamoud Alsubaie

Masters Theses

Traditional physicochemical and physicomechanical pretreatment technologies have improved the deconstruction of lignocellulose, but often require high energy inputs and added chemical reagents. Recent studies have demonstrated the use of white-rot fungi as alternative biological pretreatment agents that can mitigate these concerns. Although, white-rot fungi have been shown to grow on a variety of non-woody substrates, they have not been explored with respect to sunflower, which has the potential to provide raw materials for both biodiesel and bioethanol. In the present study, Silverleaf sunflower stems (Helianthus argophyllus) were used as substrates for the white-rot fungi Phanerochaete chrysosporium and Trametes …

Creg1: Its Role As A Master Regulator Of Liver Function, Iffat Jahan

Masters Theses

Mammalian development involves a complex system of regulatory signals and reactions resulting in highly differentiated cell types with specific structure and function. The liver is a major organ that has been studied extensively to understand underlying genetic processes responsible for specification, establishment and maintenance of tissue identity. Hepatoma and hepatoma variant cell lines have been used as models to understand genetic networks responsible for liver function. Whole genome microarray analysis of hepatocyte cell lines has revealed candidate genes that may serve as regulators or master regulators of liver specificity. In two previous studies in our lab, the role of candidate …

Mental Imagery Practice As A Therapy For Naming Impairments: A Preliminary Study, Subhash Bhatnagar, Bridget Patek, Yasmeen Khan-Ansari, Naveen K. Bansal, Hugh Buckingham, Lotfi Hacein-Bey

Speech Pathology and Audiology Faculty Research and Publications

Background

Anomia remains one of the most recalcitrant linguistic disruptions in aphasia to treat. Developing successful interventions to address the word-finding deficits are complicated by the post-stroke symptom variability and inconsistent recovery patterns associated with anomia. Most of the current treatment methods, with a focus on specific compensating techniques and the repetitive practice of a limited set of items, have had variable success in naming treatment. However, it has not been possible to predict the gains in generalizing the learning beyond the stimuli used in practice or the controlled clinical setting. In this preliminary case study, we explore the value …

Explainable Neural Networks Based Anomaly Detection For Cyber-Physical Systems, Kasun Amarasinghe

Theses and Dissertations

Cyber-Physical Systems (CPSs) are the core of modern critical infrastructure (e.g. power-grids) and securing them is of paramount importance. Anomaly detection in data is crucial for CPS security. While Artificial Neural Networks (ANNs) are strong candidates for the task, they are seldom deployed in safety-critical domains due to the perception that ANNs are black-boxes. Therefore, to leverage ANNs in CPSs, cracking open the black box through explanation is essential.

The main objective of this dissertation is developing explainable ANN-based Anomaly Detection Systems for Cyber-Physical Systems (CP-ADS). The main objective was broken down into three sub-objectives: 1) Identifying key-requirements that an …

A Teacher’S Adoption Of Maker-Centered Learning: A Phenomenological Case Study, Michael L. Schad

Theses and Dissertations

The maker movement is a social movement which espouses the importance of artifact creation through physical and digital tools. As the maker movement moves into educational spaces, researchers and educators are exploring ways to integrate it alongside current classroom practices. A prevalent framework for maker integration is the maker-centered learning framework, which was used as part of the conceptual framework. Currently, more research is needed which justifies and analyzes maker-centered learning in an educational environment. This study aimed to use phenomenological methods to capture the lived experience of chemistry teacher as she integrated the maker-centered learning framework into her chemistry …

Applications Of Chemically Modified Nitrogen Doped Carbon, Zirconium Phosphate, Metal Organic Frameworks, And Functionalized Graphene Oxide Nanostructured Adsorbents In Water Treatment, Ayyob Mohammed A Bakry

Theses and Dissertations

Water contaminations by many pollutants, especially heavy metals such as Pb(II), Hg(II), Cu(II), Cd(II), and Cr(VI) pose many public health and environmental concerns as reported in the list of hazardous substances compiled by the US Environmental Protection Agency due to their high toxicity, refractory degradation, and ease of entering food chain. Adsorption by chelating resins is proven to be the most effective method for the extraction of metal ions from polluted and wastewater. However, traditional absorbents such as activated carbon, activated alumina, clay, zeolite, etc., show limited adsorption abilities for these heavy metal ions. The major goal of this thesis …

Carbon Cycling Along The James River Continuum, Timothy B. Jones

Theses and Dissertations

Fluvial systems act as conduits for terrestrial carbon transport to coastal oceans. However, riverine systems not only transport allochthonous carbon, they transform and store carbon in many different ways. The different mechanisms of transformation and storage need to be considered for fluvial systems in order to accurately account for modifications to the carbon pool when developing carbon budgets. Of interest was to investigate the carbon dynamics in the James River, in Virginia, from headwaters to the tidal fresh estuary to gain a better understanding of carbon dynamics along the continuum. Sites along the river continuum were sampled for dissolved organic …