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Neuroticism And Stressful Life Events: Probing Mechanisms Underlying Vulnerability To Stress-Related Depression, Erin Bondy

Arts & Sciences Graduate Student Theses and Dissertations

Elevated neuroticism (N) potentiates the depressogenic effects of stressful life events (SLEs). We first replicate this association using longitudinal data (N=971 older adults) from the St. Louis Personality and Aging Network (SPAN) study. Here, SLEs prospectively predicted future depressive symptoms, especially among those reporting elevated N, even after accounting for prior depressive symptoms and previous SLE exposure (NxSLE interaction: p=0.016, ΔR²=0.003). These findings were further replicated in cross-sectional analyses of the Duke Neurogenetics Study (DNS), a young adult college sample with neuroimaging data (n=1,343: NxSLE interaction: p=0.019, ΔR²=0.003). Because evidence suggests that stress may promote depression …

Reproducibility Of Cognitive Endpoints In Clinical Trials: Lessons From Neurofibromatosis Type 1, Jonathan M Payne, David H Gutmann, Et Al

Open Access Publications

OBJECTIVE: Rapid developments in understanding the molecular mechanisms underlying cognitive deficits in neurodevelopmental disorders have increased expectations for targeted, mechanism-based treatments. However, translation from preclinical models to human clinical trials has proven challenging. Poor reproducibility of cognitive endpoints may provide one explanation for this finding. We examined the suitability of cognitive outcomes for clinical trials in children with neurofibromatosis type 1 (NF1) by examining test-retest reliability of the measures and the application of data reduction techniques to improve reproducibility.

METHODS: Data were analyzed from the STARS clinical trial (n = 146), a multi-center double-blind placebo-controlled phase II trial of lovastatin, …

Online And Clicker Quizzing On Jargon Terms Enhances Definition-Focused But Not Conceptually Focused Biology Exam Performance, Steven C. Pan, James Cooke, Jeri L. Little, Mark A. Mcdaniel, Erin R. Foster, Lisa Tabor Connor, Timothy C. Rickard

Open Access Publications

Mastery of jargon terms is an important part of student learning in biology and other science, technology, engineering, and mathematics domains. In two experiments, we investigated whether prelecture quizzes enhance memory for jargon terms, and whether that enhanced familiarity can facilitate learning of related concepts that are encountered during subsequent lectures and readings. Undergraduate students enrolled in neuroanatomy and physiology courses completed 10-minute low-stakes quizzes with feedback on jargon terms either online (experiment 1) or using in-class clickers (experiment 2). Quizzes occurred before conventional course instruction in which the terms were used. On exams occurring up to 12 weeks later, …

Linking The Effects Of Helminth Infection, Diet And The Gut Microbiota With Human Whole-Blood Signatures, Soo Ching Lee, Mei San Tang, Alice V. Easton, Joseph Cooper Devlin, Ling Ling Chua, Ilseung Cho, Foong Ming Moy, Tsung Fei Khang, Yvonne A. L. Lim, P'Ng Loke

Open Access Publications

Helminth infection and dietary intake can affect the intestinal microbiota, as well as the immune system. Here we analyzed the relationship between fecal microbiota and blood profiles of indigenous Malaysians, referred to locally as Orang Asli, in comparison to urban participants from the capital city of Malaysia, Kuala Lumpur. We found that helminth infections had a larger effect on gut microbial composition than did dietary intake or blood profiles. Trichuris trichiura infection intensity also had the strongest association with blood transcriptional profiles. By characterizing paired longitudinal samples collected before and after deworming treatment, we determined that changes in serum zinc …

Single-Cell Impedance Spectroscopy, David Paul Lange

Master's Theses

Impedance spectroscopy (IS) is an important tool for cell detection and characterization in medical and food safety applications. In this thesis, the Cal Poly Biofluidics Lab’s impedance spectroscopy system was re-evaluated and optimized for single-cell impedance spectroscopy. To evaluate the IS system, an impedance spectroscopy bioMEMS chip was fabricated in the Cal Poly Microfabcrication lab, software was developed to run IS experiments, and studies were run to validate the system. To explore IS optimization, Maxwell’s mixture theorem and the Schwartz-Christoffel transform were used to calculate an analytic impedance solution to the co-planar electrode system,a novel volume fraction to account for …

Building Skills For And Implementing Course Based Undergraduate Research Experiences (Cures) Across An Environmental Studies Curriculum, Alanna L. Lecher, Cassandra S. Korte

Faculty and Staff Publications & Presentations

No abstract provided.

Quantification Of Palmer Amaranth Seed Number Using A Computerized Particle Analyzer, Matthew B. Bertucci, Paul C. Bartley Iii, Katherine M. Jennings, David W. Monks, Brian E. Jackson

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

We evaluated the accuracy of a computerized particle analyzer (CPA) for high-throughput counting of Palmer amaranth (Amaranthus palmeri S. Watson) seeds and subsequently used the CPA to verify the accuracy of two subsampling methods for estimation of Palmer amaranth seed production. To determine accuracy of the CPA, 55 hand-counted samples, ranging from 500 to 5000 Palmer amaranth seeds, were drawn from field samples and counted with the CPA. The relationship between hand and CPA seed counts was described by a linear model (R-2 = 0.99) with a slope of 0.987 and a y-intercept of 3.49. Thus, very little discrepancy exists …

A Reinforcement Learning Approach To Spacecraft Trajectory Optimization, Daniel S. Kolosa

Dissertations

This dissertation explores a novel method of solving low-thrust spacecraft targeting problems using reinforcement learning. A reinforcement learning algorithm based on Deep Deterministic Policy Gradients was developed to solve low-thrust trajectory optimization problems. The algorithm consists of two neural networks, an actor network and a critic network. The actor approximates a thrust magnitude given the current spacecraft state expressed as a set of orbital elements. The critic network evaluates the action taken by the actor based on the state and action taken. Three different types of trajectory problems were solved, a generalized orbit change maneuver, a semimajor axis change maneuver, …

Negotiating American Racial Constructs: First- Generation African Caribbean Immigrants’ Experience With Race, Rommel Johnson

Dissertations

The purpose of this study is to describe and understand what race means to first-generation African Caribbean immigrants. Specifically, the study seeks to understand these immigrants’ experience of being Black in America. African Caribbean immigrants migrate to America with a concept of race that is very different from that of the United States. However, upon arriving in America, they encounter the American construct of race that not only diverges significantly from that with which they are familiar, but find that they are being racialized in the American sense. In professional counseling, we have yet to understand the way these immigrants …

Hippocampal Extracellular Potassium Levels And Formation Of Spatial Memory In Response To Retrodialysis Insulin Administration, Gabrielle Shames

Anthropology

Insulin is the most common treatment for hyperglycemia, such as that caused by type 1 or type 2 diabetes mellitus. Insulin causes cellular uptake and storage of glucose to maintain homeostasis and also plays important roles in other systems; an important example is regulation of potassium. In the periphery, insulin administration has been shown to increase the cellular uptake of potassium via Na+/K+ ATPase, leading to hypokalemia. Research in our lab and others has shown that insulin is a key regulator of cognitive function and local metabolism within the hippocampus. To date, however, no studies have examined whether insulin acts …

Improving Grapheme-To-Phoneme Translation With The Use Of Nalu Attention Mechanisms, Brian Sheldon Smith

Theses and Dissertations

In recent years, Natural Language Processing in the field of machine learning has seen some major improvements. Data scientists have shown that neural networks are capable of breaking down semantics of sentences, translating languages, and answering complex questions with fast recall. While impressive, these feats all hinge on having access to a massive amount of clean text, or data sets with almost perfect grammar and spelling. Without this, neural networks will usually fail to converge on a meaningful result. To partially this dependency, Grapheme-to-Phoneme conversion can be employed. This is the conversion of words from their spellings to a form …

Ultrafast Relaxation Dynamics In Graphene Oxide-Dye And Perovskites Nanocomposites, Abubkr A. Arzaq Abuhagr

Dissertations

Novel materials such as graphene oxide (GO), reduced graphene oxide (RGO), and Perovskites nanocomposites nanosheets have shown interesting electrical and optical properties. These materials have shown prominence in research regarding optical sensing applications. The interaction of different fluorescent molecules like dye molecules with GO, and RGO have been studied recently to develop novel optical sensors, photo-catalysts, and light-harvesting agents. In this study, we have monitored the excited state interactions of dyes covalently attached to GO and RGO nanosheets. Three amine derivatives of anthracene, pyrene, and coumarin were covalently bound to different systems via amide bonds and diazotization. Characterization of different …

Smart Sensors With Dual Modes Of Signal Transduction For Monitoring Molecules Pertinent To Health And The Environment, Jared T. Wabeke

Dissertations

The dissertation focuses on the design and synthesis of smart materials for the detection of molecules pertinent to environmental protection and healthcare. The use of computational simulations is pivotal toward advancing molecular design for targeted applications. Research was conducted to investigate the use of simulations to develop novel sensors with dual modes of signal transduction. The molecular properties were determined using computational modelling, and then used to elucidate the binding mechanism of the corresponding sensor complexes. Several molecules were produced that respond to important organic analytes, such as glucose and fenthion, an organophosphorus pesticide. Glucose is an exceedingly important biological …

Semantic And Qualitative Physics-Based Formal Reasoning For Functional Decomposition In Mechanical Design, Xiaoyang Mao

Theses and Dissertations

Function modeling plays an essential role in academy design studies, yet a lack of acceptance in industry. A possible reason is that the designer must have well understand of the controlled vocabularies and grammars to utilize this method. The research presented in this dissertation is to fill this gap so that designers can use function-based design method without relevant knowledge. In graph-based function models, the function verbs and flow nouns are usually chosen from predefined vocabularies. The vocabulary class definitions, combined with function modeling grammars defined at various levels of formalism, enable function-based reasoning. However, the text written in plain …

Response To Commentaries On Who’S The Bigot?, Linda C. Mcclain

Faculty Scholarship

One of the joys of writing a book is the chance to have its arguments and observations evaluated by creative and engaged readers. I am very grateful that the scholars included in this book symposium provided such constructive commentary on the manuscript of my book, Who’s the Bigot? Learning from Conflicts over Marriage and Civil Rights Law. One of those commentators, Professor Imer Flores, also generously hosted a wonderful live conference at which I had the chance to hear and engage with early versions of several of these commentaries. The final book, I hope, reflects improvements that grew out of …

Investigating Administrators’ Resistance To Implementing Restorative Practices, Beri Deister

Electronic Theses and Dissertations

In this case study, the researcher investigated the barriers to school administrators’ implementation of restorative practices as a management approach to student behavior. Based on the claim that restorative practices is a promising alternative to exclusionary disciplinary placements for changing behavior, the researcher used an emergent explanatory mixed methods design to better understand administrators’ resistance to change despite overwhelming evidence that exclusionary disciplinary practices are the primary contributors to national trends in disciplinary disproportionality and institutional discrimination. The researcher identified and analyzed barriers to create recommendations for addressing administrators’ resistance through organizational change strategies using systems theory.

Evaluation Of Detached Strawberry Leaves For Anthracnose Disease Severity Using Image Analysis And Visual Ratings, Melinda A. Miller-Butler, Barbara J. Smith, Kenneth J. Curry, Eugene K. Blythe

Faculty Publications

Inoculation of detached strawberry leaves with Colletotrichum species may provide an accurate, rapid, nondestructive method of identifying anthracnose-resistant germplasm. The purpose of this study was to statistically compare two methods (visual and image analysis) of evaluating disease severity of strawberry germplasm screened for anthracnose resistance. Detached leaves of 77 susceptible and resistant strawberry clones were inoculated with one Colletotrichum gloeosporioides (Penz.) Penz. & Sacc. and two C. fragariae A. N. Brooks isolates. Anthracnose disease symptoms on each leaf were assessed quantitatively via computer-based image analysis to determine percentage lesion area and qualitatively by two independent raters using a visual disease …

Distinguishing Anuran Species By High-Resolution Melting Analysis Fo The Coi Barcode (Coi-Hrm), Steven Everman, Shiao Y. Wang

Faculty Publications

Taxonomic identification can be difficult when two or more species appear morphologically similar. DNA barcoding based on the sequence of the mitochondrial cytochrome c oxidase 1 gene (COI) is now widely used in identifying animal species. High‐resolution melting analysis (HRM) provides an alternative method for detecting sequence variations among amplicons without having to perform DNA sequencing. The purpose of this study was to determine whether HRM of the COI barcode can be used to distinguish animal species. Using anurans as a model, we found distinct COI melting profiles among three congeners of both Lithobates spp. and Hyla spp. Sequence variations …

Bipolar Fresh Osteochondral Allograft Transplantation And Joint Reconstruction For Patellar And Trochlear Cartilage Defects, Omid Jalali, Zachary Vredenburgh, John Prodromo, Neilen Benvegnu, George Frederick Rick Hatch

Faculty, Staff and Students Publications

Patellar instability is a debilitating condition, and trauma due to the dislocations may severely damage the articular cartilage of the patellofemoral joint. The lack of healing capacity of cartilage makes treatment of such lesions challenging. In young patients, preservation of native bone and cartilage is a priority, and therefore arthroplasty procedures should be avoided even in relatively larger lesions. Fresh osteochondral allografting has been described for these difficult and complex cases, with recent studies showing promising results. In this surgical technique, we describe the use of bipolar osteochondral allografts of the patella and trochlea and patellofemoral joint reconstruction for large …

Gna11 Brain Somatic Pathogenic Variant In An Individual With Phacomatosis Pigmentovascularis, Joseph M Sliepka, Sarah C Mcgriff, Linda Z Rossetti, Peyman Bizargity, Haley Streff, Yi-Shan Lee, Hongzheng Dai, Satyamaanasa Polubothu, Grace Lee, Vicky Ren, Jill V Hunter, Daniel J Curry, Fernando Scaglia, Adekunle M Adesina, Irfan Ali, Veronica Kinsler, Lindsay C Burrage, Dana Marafi

Faculty, Staff and Students Publications

OBJECTIVE: To describe the findings of histopathology and genotyping studies in affected brain tissue from an individual with phacomatosis pigmentovascularis (PPV).

METHODS: A retrospective chart review of a 2-year 10-month-old male with a clinical diagnosis of PPV cesiomarmorata (or type V) was performed. Clinical features, brain imaging and histopathology findings, and genotyping studies in his affected brain tissue are summarized.

RESULTS: The proband had a clinically severe neurologic phenotype characterized by global developmental delay, generalized hypotonia, and recurrent episodes of cardiac asystole in the setting of status epilepticus. A somatic pathogenic variant in

CONCLUSIONS: The finding of this variant in …

Structural Basis Of Amino Acid Surveillance By Higher-Order Trna-Mrna Interactions, Shuang Li, Zhaoming Su, Jean Lehmann, Vassiliki Stamatopoulou, Nikoleta Giarimoglou, Frances E Henderson, Lixin Fan, Grigore D Pintilie, Kaiming Zhang, Muyuan Chen, Steven J Ludtke, Yun-Xing Wang, Constantinos Stathopoulos, Wah Chiu, Jinwei Zhang

Faculty, Staff and Students Publications

Amino acid availability in Gram-positive bacteria is monitored by T-box riboswitches. T-boxes directly bind tRNAs, assess their aminoacylation state, and regulate the transcription or translation of downstream genes to maintain nutritional homeostasis. Here, we report cocrystal and cryo-EM structures of Geobacillus kaustophilus and Bacillus subtilis T-box-tRNA complexes, detailing their multivalent, exquisitely selective interactions. The T-box forms a U-shaped molecular vise that clamps the tRNA, captures its 3' end using an elaborate 'discriminator' structure, and interrogates its aminoacylation state using a steric filter fashioned from a wobble base pair. In the absence of aminoacylation, T-boxes clutch tRNAs and form a continuously …

The Role And Effect Of Mindfulness In Intimate Relationships, Mazyar Karandish

Antioch University Dissertations & Theses

Mindfulness practice, a present-centered approach to experience, has been shown to effectively enhance various dimensions of the human experience. Recent literature has explored how the practice of mindfulness meditation has provided benefits to practitioners seeking to enhance relationship dynamics. There are multiple examples of mindfulness-based relationship enhancement programs aiming to enhance the quality of intimate relationships in adults. This study utilizes a repeated-measures design to address whether a neutral mindfulness-based program, not geared at enhancing relationships, can significantly alter the quality of intimate relationships. The research will further examine the central mechanisms of action through which mindfulness can enhance relationships. …

Peach Twig Borer Mating Disruption, Marion Murray, Diane G. Alston

All Current Publications

Mating disruption became commercially available in the early 1990s, and was adopted by many Utah growers about a decade later. Use of this pest management technology can be daunting due to high up-front costs and monitoring requirements; however, after two to three seasons of use, the cost of mating disruption is the same or even less than conventional pest management.

Hvac System Energy Audit For Leverett Elementary School, Connor Smalling

Biological and Agricultural Engineering Undergraduate Honors Theses

Leverett Elementary School is located in Fayetteville, AR. The school needs significant upgrades to its infrastructure. The Fayetteville Public School District has voted to pursue an Energy Services Performance Contract (ESPC) in order to finance the desired upgrades to Leverett Elementary, among other schools in the district.

The scope of this thesis was to perform an energy audit on the existing heating, ventilation, and air conditioning (HVAC) system. By using an energy modeling software, eQuest, the building and the existing base system were modeled to determine utility consumption. Three different HVAC system alternatives were analyzed against the base system by …

Drivers Of On-Farm Performance Of Irrigation Water Management Practices: Empirical Evidence From Eastern Arkansas, Jacob Askey

Biological and Agricultural Engineering Undergraduate Honors Theses

Unsustainable agricultural practices are putting a strain on freshwater supplies in many parts of the world. More efficient irrigation techniques are going to be critical to feed a growing population. Data from the 2016 Arkansas Irrigation Survey was used to assess the adoption of three Irrigation Water Management (IWM) practices: multiple inlet rice irrigation, tailwater recovery, and storage reservoirs. Results indicate that these practices do not always lead to reported pumping time reductions, a proxy for water use savings. Large variations in pumping time reduction are observed across producers for all three practices. A Tobit model was used to examine …

Cardiac Damage By Doxorubicin Is Mitigated In Smad3 Deficient Mice, Kyle Campbell, Edward Dorfman, James Yang, Melissa S. Cobb, Magdy Girgis, Jeryl Hauptman, Shixin Tao, Robert Vincent, Dawn Buddhadeb, Eugene A. Konorev

Student Publications

Statement of Significance: Cardiovascular disease is the leading cause of morbidity and mortality in long-term cancer survivors. Chemotherapy with doxorubicin and other anthracyclines is the most common cause of cardiac toxicity in treated cancer patients. These agents are used to manage both hematologic and solid-tumor malignancies. Cardiac manifestations often develop months to years after initial exposure, causing cardiomyopathy and heart failure. Osteopathic medicine approaches disease in a holistic manner attempting to prevent major complications earlier in the disease course. Preventing or reducing chemotherapy-induced cardiotoxicity would greatly improve the outlook for these patients and their overall wellbeing.

Methods: Smad3+/+ wild type …

Nanoscale Optical And Correlative Microscopies For Quantitative Characterization Of Dna Nanostructures, Christopher Michael Green

Boise State University Theses and Dissertations

Methods to engineer nanomaterials and devices with uniquely tailored properties are highly sought after in fields such as manufacturing, medicine, energy, and the environment. The macromolecule deoxyribonucleic acid (DNA) enables programmable self-assembly of nanostructures with near arbitrary shape and size and with unprecedented precision and accuracy. Additionally, DNA can be chemically modified to attach molecules and nanoparticles, providing a means to organize active materials into devices with unique or enhanced properties. One particularly powerful form of DNA-based self-assembly, DNA origami, provides robust structures with the potential for nanometer-scale resolution of addressable sites. DNA origami are assembled from one large DNA …

Predation Of Eastern Cottontail Rabbit (Sylvilagus Floridanus) By Great Blue Heron (Ardea Herodias), Carlos E. Cintra-Buenrostro, J. E. Cifuentes-Lujan

School of Earth, Environmental, & Marine Sciences Faculty Publications

An opportunistic predation by Great Blue Heron (Ardea herodias) on Eastern cottontail rabbit (Sylvilagus floridanus) was observed in south Texas on 31 Oct 2019. The Great Blue Heron had already captured the Eastern cottontail rabbit at the first observation but the maneuvering process, killing, and ingestion were recorded photographically, which make this observation unique even though this might constitute the second report on Great Blue Heron eating Eastern cottontail rabbits.

Exploration Of Nucleic Acid Hydrogran Bonding Using Molecular Mechanics And Dft Calculations, Simi Kaur

Biological Sciences

Many recent theoretical and experimental techniques have been developed to probe the structurefunction relationships of complex biomolecules. The roles of RNAs are dependent upon various intricate structural motifs and interactions, including hairpins, pseudoknots, long range territory contacts, bulges and internal loops, that are not easily captured by these methods. We had previously developed an enhanced replica exchange molecular dynamics method that incorporated secondary structure information in the form of distance restraints in order to effectively overcome kinetic barriers and sample conformational space. In several structures, restrained RNA base pairs near large bulges displayed a preference for stacking over hydrogen bonding …

Embedded Silver Nanoparticles For Metal Enhanced Photoluminescence, Shahid Iqbal

Dissertations

Imaging of biologically significant molecules using plasmons of Metal Nanoparticles (MNPs) is gaining attention in the research community. Localized Surface Plasmon Resonance (LSPR) is the coherent oscillation of conduction electrons of MNPs. The biologically significant molecule is labeled with the fluorophore molecule to get the image. This approach is widely used in clinical practices, however, low intensity light emission from the labeled molecule makes it difficult to image the biologically significant material. One way to improve the weak intensities of fluorophore is to enhance the brightness using a process called Metal Enhanced Photoluminescence (MEP). This phenomenon occurs in the close …