Digital Commons Network^™

Synthesis Of Cellulose Nanocrystal-Gold Nanoparticle Hybrid System For Surface Plasmon-Enhanced Property, Mahshid Iraniparast

Graduate Theses and Dissertations

Gold nanoparticles (AuNPs) have been brought to the forefront of various applications, ranging from theranostics, to organic photovoltaics, to biosensing owing to their localized surface plasmon resonance (LSPR) property. However, this property needs to be improved in order to allow for high sensitivity and quantitative detection of biomolecules. Hybrids of AuNPs with low-dielectric cellulose nanocrystal (CNCs) would yield enhancement of the LSPR property, which is driven by the confinement of electron oscillation at their interfaces. This study proposed a seed-mediated growth method to synthesize hybrids of CNCs-AuNPs. Sulfate groups on the surface of CNCs served as the sites for the …

Detecting Differentially Co-Expressed Gene Modules Via The Edge-Count Test, Anne Gratius Lin

Graduate Theses and Dissertations

Background

Gene expression profiling by microarray has been used to uncover molecular variations in many different diseases. Complementary to conventional differential expression analysis, differential co-expression analysis can identify gene markers from the systematic and granular level. There are three aspects for differential co-expression network analysis, including the network global topological comparison, differential co-expression cluster identification, and differential co-expressed genes and gene pair identification. To date, most of the methods available still rely on Pearson’s correlation coefficient despite its nonlinear insensitivity.

Results

Here we present an approach that is robust to nonlinearity by using the edge-count test for differential co-expression analysis. …

Applications Of Ionic Liquids In Membrane Separation, Mohanad Kamaz

Graduate Theses and Dissertations

Ionic liquids represent an emerging attractive material in membrane technology. The central theme of this doctoral dissertation is to develope novel membranes using ionic liquids. Two different approaches were used to prepare ionic liquid membranes including the immobilization of ionic liquid within the membrane pores or the use of pressure assembly method to deposit a selective ionic liquid layer on the top membrane surface.

In chapter 2, imidazolium ionic liquids with three different alkyl halides were successfully synthesized and used to prepare supported ionic liquid membranes (SILMs). SILMs preraper were tested for aqueous and nonaqueous applications. For nonaqueous applications, the …

Quantitative Study Of The Antimicrobial Effects Of Silver On The Motility Of Escherichia Coli, Benjamin Russell

Graduate Theses and Dissertations

In recent decades, the number of antibiotic-resistant bacterial infections has grown to become a serious global threat. This rise can be attributed to the widespread misuse of antibiotics and the lack of newly developed drugs to fight resistant organisms. Novel bactericidal substances have, therefore, garnered significant research interest. Silver, due to its powerful antimicrobial effects, is one such substance. Silver is typically most effective in cationic form; however, advancements in nanotechnology have paved the way for the controlled fabrication of nano-silver. Silver nanoparticles have been shown to have increased antibacterial potency for a variety of reasons, including the release of …

Serum Myostatin And Igf-1 As Biomarkers Of Sarcopenia: A Proof-Of-Concept Design, Ashley Binns

Graduate Theses and Dissertations

The population comprising older adults is growing exponentially, as are healthcare related costs. Nearly $20 billion is annually expensed by older adults for health-related issues affiliated with age-related decline in skeletal muscle mass. Yet, diagnostic criteria are not readily utilized in clinical practice. PURPOSE: Therefore, the purpose of this study was to provide evidence for use of blood biomarkers (myostatin, IGF-1) to predict appendicular skeletal muscle mass (ASM) among middle-aged and older adults. METHODS: A cross-sectional study was conducted among 106 individuals (n = 68 females; n = 38 males) aged ≥ 40 years of age (60.1 ± 11.1 y) …

The Physicochemical Properties Of Soy Protein Hydrolysate And Its Formulation And Stability With Encapsulated Probiotic, John Stricklin Edwards

Graduate Theses and Dissertations

Soy protein isolate (SPI) and probiotics can serve as a functional ingredient and beneficial supplement, respectively, to food formulations, but few studies focus on the compatibility of these two ingredients. The objective of this study was to prepare soy protein isolate (SPI) from defatted soybean flour and identify an optimal hydrolysis protocol to create soy protein hydrolysates (SPH) with improved solubility and functional properties and achieve an optimum encapsulation technique for probiotics and to determine the in vitro viability of encapsulated probiotic cells with SPH in a gastrointestinal environment. The SPI was prepared using an alkaline extraction procedure for solubility …

Created For Connection: The Impact Of A Faith-Based Christian Marriage Enrichment Program, Chad N. Imhoff

Graduate Theses and Dissertations

The purpose of this study was to explore the effects of Created for Connection (CFC), a faith based marriage enrichment program on relationship satisfaction, adult attachment style and adult attachment behavior. This study further adds to the existing knowledge base related to faith-based programs delivered in an intensive weekend format (Johnson & Sanderfer, 2016). Participants were volunteers from the local community who identified as Christian. To ascertain the effectiveness of CFC, three self-report measures were administered at three different intervals: two weeks before the program, two weeks following the program and a six-week follow-up. I measured attachment anxiety, attachment avoidance, …

Aglycon Modifications Of Ipomoeassin F: Synthetic Route Development And Analog Synthesis To Enable Further Sar Studies, Eric Barber

Graduate Theses and Dissertations

The ipomoeassin family of resin glycosides were discovered to have a high potency against numerous cancer cell line, with ipomoeassin F being the most potent among the family of natural products. Interestingly, one of the few differences between ipomoeassin F and the other compounds is the length of the fatty-acid derived aglycon. As the mechanism of action for this family of resin glycosides is unknown and didn’t have any significant COMPARE correlation with the recorded anticancer agents in the National Cancer Institute (NCI), further SAR studies are needed. Drawing on the differences between ipomoeassin F and the other ipomoeassins, it …

Climbing The Corporate Ladder And Within-Person Changes In Narcissism: Reciprocal Relationships Over Two Decades, Bart Wille, Joeri Hofmans, Filip Lievens, Mitja D. Back, Filip De Fruyt

Research Collection Lee Kong Chian School Of Business

Prior research demonstrated that narcissism fosters the attainment of higher managerial ranks in organizations. However, it is not known whether climbing the corporate ladder also fosters the development of narcissism over time. Whereas prior work consistently adopted a unidirectional perspective on narcissism and career attainment, this study presents and tests a bidirectional perspective, incorporating long-term development in narcissism in relation to and in response to long-term upward mobility. To this end, a cohort of highly educated professionals was assessed three times over a 22-year time frame. Extended latent difference score modeling showed that, over the entire interval, within-person changes in …

Cytotoxic Effects Of Vitamin D3 On Tumor Cell Lines, Twana A. Mustafa, Iman M. Rasul

Polytechnic Journal

Vitamin D3 is a potent antiproliferative agent against various tumor cells in vitro. Here, the results of Vitamin D3 study as a potential antitumor therapy in vitro are presented. Applying antiproliferative 3(4,5-dimethyl- 2-thiazolyl)-2,5-diphenyl-2H-terazolium bromide assays, the inhibitory effects of the Vitamin D were measured. The following cancer cell lines were employed: L20B (normal cell line) and RD (malignant rhabdomyosarcoma). Both cell lines were cultivated in 96-wells culture plates in the presence and absence of different doses of Vitamin D (10–6, 10–8, and 10–10 ?g/ml) for 24 and 48 h. In vitro results of cytotoxic effects were variable on both cell …

Identification Of The Kaposi's Sarcoma-Associated Herpesvirus (Kshv) Surface Glycoprotein Targets Of Human Kshv-Specific Neutralizing Antibody Responses, Yasaman Mortazavi

School of Biological Sciences: Dissertations, Theses, and Student Research

Kaposi’s sarcoma-associated herpesvirus (KSHV), also known as human herpesvirus 8 (HHV-8), is the etiological agent of Kaposi’s sarcoma (KS), and is also associated with two B cell malignancies, primary effusion lymphoma and multicentric Castleman's disease. The distribution of KSHV varies globally with high prevalence in some areas of sub-Saharan Africa (SSA), where seroprevalence can be as high as 80%. It is estimated that nearly 44,000 new cases of KS emerge annually globally, with the highest incidents occurring in Africa, where KSHV is endemic. Currently, there is no prophylactic vaccine against KSHV, and efforts to develop prophylactic vaccines have been limited. …

Crossover Interference And Sex-Specific Genetic Maps Shape Identical By Descent Sharing In Close Relatives, Madison Caballero, Daniel N. Seidman, Ying Qiao, Jens Sannerud, Thomas D. Dyer, Donna M. Lehman, Joanne E. Curran, Ravindranath Duggirala, John Blangero, Shai Carmi, Amy L. Williams

School of Medicine Publications

Simulations of close relatives and identical by descent (IBD) segments are common in genetic studies, yet most past efforts have utilized sex averaged genetic maps and ignored crossover interference, thus omitting features known to affect the breakpoints of IBD segments. We developed Ped-sim, a method for simulating relatives that can utilize either sex-specific or sex averaged genetic maps and also either a model of crossover interference or the traditional Poisson model for inter-crossover distances. To characterize the impact of previously ignored mechanisms, we simulated data for all four combinations of these factors. We found that modeling crossover interference decreases the …

Electronic Word-Of-Mouth (Ewom): Examining The Relationship Between Factors Influencing Ewom Activities And Passengers’ Purchasing Involvement In The Context Of Aviation, Ratchada Ruenruoy

Theses and Dissertations

The purpose of the study was manifold: (a) to examine the relationship a set of targeted factors has on electronic Word-of-Mouth (eWOM) activities in the context of aviation, (b) to examine the extent to which these factors affect airline passengers’ purchasing involvement, and (c) to determine the extent to which eWOM activities mediate the relationship between the targeted factors and purchasing involvement. Research factors included (a) purchasing perceptions, which consisted of satisfaction, loyalty, perceived value, and service quality; (b) social environment, which consisted of age, gender, and cultural differences relative to Hofstede’s (1980) cultural dimensions; and (c) experiences, which consisted …

Optical Detection Of The Structural Properties Of Tumor Tissue Generated By Xenografting Of Drug-Sensitive And Drug-Resistant Cancer Cells Using Partial Wave Spectroscopy (Pws), Prakash A. Adhikari, Prashanth K.B. Nagesh, Fatemah Alharthi, Subhash C. Chauhan, Meena Jaggi, Murali M. Yallapu, Prabhakar Pradhan

School of Medicine Publications

A mesoscopic physics-based optical imaging technique, partial wave spectroscopy (PWS), has been used for the detection of cancer by probing nanoscale structural alterations in cells/tissue. The development of drug-resistant cancer cells/tissues during chemotherapy is a major challenge in cancer treatment. In this paper, using a mouse model and PWS, the structural properties of tumor tissue grown in 3D structures by xenografting drug-resistant and drug-sensitive human prostate cancer cells having 2D structures, are studied. The results show that the 3D xenografted tissues maintain a similar hierarchy of the degree of structural disorder properties as that of the 2D original drug-sensitive and …

Elementary School Administrators’ Perspectives Of Expanded School Mental Health Systems And Implications For Further Training, Melissa M. Petersen

Student Work

School administrators play a crucial role in the development and implementation of mental health systems that allow all students access to universal, preventative interventions and services. Understanding their perspectives about the fundamental features of effective Expanded School Mental Health systems is critical to understanding the research to practice gap as it relates to the successful implementation of school-based mental health services. Furthermore, it is necessary to understand administrators’ perspectives about the training and supports teachers need to increase knowledge, confidence, and self-efficacy in the delivery of effective school mental health services. The purpose of this pragmatic qualitative research study was …

The Classic, Winter 2019-2020, Public Relations

The Classic, 2010-2019

Zwemer View: Standout Courage and Faithfulness; Around the Green: Award-Winning Giving, In Box, Returning to Serve, HR MVPs, PA Program Gets Green Light, Schools for Syria, Campus Life: Dressember, Podcast for Educators, Extra! Extra!, Enrollment Sets Record, NWC Bandwidth Usage, Leaving NWC Financially Strong, Science Center Plaza, The Story Continues, Face Value: Tom Truesdell, Forever a Raider, Excellent Affirmed, Raider Nation Celebration, Bridge Builder, Comfort Keeper, Distinguished Alumni; Giving to Northwestern College in 2018-19; Red Zone: Double Threat; Looking Back: Closed Call; 1,000 Words: Making a Statement; Big Times in the Bultman: 10 of the Best Moments on the Raiders' …

Customizable Data Visualization Of Wnt Signaling, Morgan Lee King

Computer Science Honors Theses

As technology advances, biologists are able to obtain more genetic information from experiments than ever before. As the amount of data they produce continues to increase, it is becoming more difficult to process the information and produce results that can be used in biological and medical research. One of the simplest ways to parse information quickly is through visualization. This project aims to improve the readability and utility of graph visualizations for biological pathway analysis by adding interactive and customizable components that allow biologists to determine what view of a dataset is most helpful to answer their questions.

This visualization …

The Prairie Naturalist, Vol. 51, Issue 2, December 2019

The Prairie Naturalist

45 EDITOR’S NOTE: Final Thoughts as Editor-in-Chief, by Christopher N. Jacques

RESEARCH ARTICLES

47 Bats of the Loess Hills Ecoregion of Southeast Nebraska, by Virgil Brack, Jr., Dale W. Sparks, and Darwin C. Brack

58 Serological Survey and Pathogen Exposure of Adult Female White-tailed Deer in the Western Dakotas, by Katherine L. Moratz, Bailey S. Gullikson, Eric S. Michel, Daniel M. Grove, Jonathan A. Jenks, and William F. Jensen

68 Factors Limting Reintroduced Plains Topminnow, Fundulus sciadicus, Populations in Central Great Plains Streams, by Joseph D. Thiessen, Keith D. Koupal, and Casey W. Schoenebeck

NOTES

77 Recent Observations of Water …

Factors Limiting Reintroduced Plains Topminnow, Fundulus Sciadicus, Populations In Central Great Plains Streams, Joseph Thiessen, Keith D. Koupal, Casey W. Schoenebeck

The Prairie Naturalist

The plains topminnow (Fundulus sciadicus) is an endemic Great Plains stream fish that has experienced declines in geographic range and local abundance. Due to these declines, the species has been considered for federal protection and designated with conservation status in states throughout its historic range. The reasons for declines are likely similar to hypothesized factors for other endemic stream fish declines in the Great Plains. To investigate potential limiting factors a suite of 17 historic sites with reintroduced plains topminnow populations across Nebraska were evaluated for current populations and if plains topminnow were absent, additional fish were introduced. …

Review Of Grasslands And Climate Change By David J. Gibson And Jonathan A. Newman, Neal D. Noemuth

The Prairie Naturalist

The last decade has seen an explosion of information about climate change, some of which is contradictory, much of which is confusing, and the entirety of which is too much for the typical biologist or scientist to assemble and comprehend. This is why reviews such as Grasslands and Climate Change, edited by David Gibson and Jonathan Newman, are so valuable. To produce this review of climate change issues and influences relative to grasslands, Gibson and Newman recruited 30 scientists—predominantly from Europe and North America—who wrote 19 chapters dealing with various aspects of grasslands and climate change. The chapters are grouped …

Anti-Inflammatory Effects Of Cruciferous And Apiaceous Vegetables In C57bl/6j Mice Colon, Rosa Moreno Narvaez

Graduate Theses and Dissertations

Previous studies have demonstrated chemo-preventive potential of cruciferous and apiaceous vegetables against colon cancer. Colon inflammation is one condition closely related with colon cancer initiation. Therefore, we wanted to compare if total western diet (TWD) was as pro-inflammatory as died-induced obesity (DIO) and a better dietary model for human conditions, and determine if diet supplementation with cruciferous (broccoli, watercress and cabbage) or apiaceous vegetables (celery and parsnip) could reduce dietary inflammation, and which vegetable was more effective.

Male CBL57/6J mice were fed chow for seven days, on day eight, mice were assigned to one of the following diets: American Institute …

Qtl Mapping Of Panicle Architecture And Yield-Related Traits Between Two U.S. Rice Cultivars 'Lagrue' And 'Lemont', Adam D. Rice

Graduate Theses and Dissertations

Grain yield in rice consists of multiple yield components such as number of panicles/plant, number of tillers/plant, and number of seeds/panicle. Panicle architecture is an important yield trait yield as the number of seeds on a panicle greatly contributes to the overall panicle yield. A QTL analysis and mapping study on panicle architecture and yield-related traits was performed to identify major QTL and candidate genes associated with the traits. The first objective of the project was to do a yield study evaluating 15 agronomic traits between each of the four U.S. rice cultivars, ‘LaGrue’, ‘Lemont’, ‘Bengal’, and ‘Mars’, to determine …

Rotational Tuning Of Transmembrane Helix Properties Based On The Precise Placements Of Aromatic And Charged Residues, Matthew J. Mckay

Graduate Theses and Dissertations

Designed model transmembrane peptides and oriented 2H and 15N solid-state nuclear magnetic resonance (NMR) spectroscopy were used to analyze how simple sequence modifications can influence peptide structure, behavior and dynamics as well as for determining the pKa of glutamic acid at the membrane interface. The GW5,19ALP23 (acetyl-GGALW(LA)6LWLAGA-amide) peptide framework adopts a well-defined tilted orientation in lipid bilayers (DLPC, DMPC and DOPC) and undergoes low amounts of dynamic motion. The sequence was initially modified by moving the Trp residues outwards to positions 4 and 20. This new sequence GW4,20ALP23 (acetyl-GGAW(AL)7AWAGA-amide) displays high amounts of signal averaging of NMR observables caused by …

Global Acetylation Dynamics In The Heat Shock Response Of Saccharomyces Cerevisiae, Rebecca E. Hardman

Graduate Theses and Dissertations

All organisms face a constant barrage of environmental stresses. Single-cell organisms such as Saccharomyces cerevisiae, or common Baker’s yeast, must rely solely on cellular responses in order to survive. This response must occur in a rapid and highly coordinated manner to quickly inhibit all unnecessary processes and shuttle all available resources to those necessary for survival. One method that cells utilize for rapid protein regulation is the use of post-translational modifications. Enzymes within the cell add or remove a variety of chemical modifications, thus altering the local chemical environment of a protein. This creates a conformational change in the protein …

Single Molecule Fluorescence Studies Of Protein Structure And Dynamics Underlying The Chloroplast Signal Recognition Particle Targeting Pathway, Dustin R. Baucom

Graduate Theses and Dissertations

The work presented in this dissertation explores the structural dynamics in the chloroplast signal recognition particle pathway. Findings include cpSRP shows scanning functionality similar to that in the cytosolic SRP with the ribosome. The intrinsically disordered C-terminal tail of the Albino3 protein has some transient secondary structure. Upon binding to cpSRP43 in solution, separate secondary structure formation was identified in the C-terminal tail of Albino3. Finally, to increase efficiency of analyzing fluorescence time traces for this work, a modular software was produced.

Influence Of Single And Multiple Histidine Residues And Their Ionization Properties On Transmembrane Helix Dynamics, Orientations And Fraying, Fahmida Afrose

Graduate Theses and Dissertations

Since aromatic and charged residues are often present in various locations of transmembrane helices of integral membrane proteins, their impacts on the molecular properties of transmembrane proteins and their interactions with lipids are of particular interest in many studies. In this work, I used solid-state deuterium NMR spectroscopy in designed model peptide GWALP23 [GGALW(LA)6LWLAGA] with selective deuterium labels to addresses the pH dependence and influence of single and multiple “guest” histidine residues in the orientation and dynamic behaviors of transmembrane proteins. The mutations include Gly to His (G2/22 to H2/22), Trp to His (W5/19 to H5/19) and Leu to His …

Investigating Egg Storage Conditions, Hatch Characteristics, And Feeding Methods Of Commercially Produced Poultry, Joshua R. Deines

Graduate Theses and Dissertations

Advancements in poultry research continue to add knowledge and understanding to an already technical industry. Such information has led to new developments in early feeding strategies which provide nutrients to broiler chicks while in the egg or during transport. This wealth of knowledge in poultry research extends beyond chickens and is utilized for other species, such as quail and pheasants, including how fertile eggs are stored and incubated. However, methods of early feeding broilers have not been investigated in industry relevant approaches to understand its effects, including how timing of feed access interacts with the hatch window. As well, quail …

Nematode Populations As Affected By Residue And Water Management In A Long-Term Wheat-Soybean Double Crop In Eastern Arkansas, Lucia Emperatriz Escalante Ortiz

Graduate Theses and Dissertations

Soybeans (Glycine max) are one of the major row crops in the United States, particularly in Arkansas. Soybean cyst nematode (SCN, Heterodera glycines) and southern root-knot nematode (RKN, Meloidogyne incognita) are two of the most damaging pests that cause major economic losses in soybeans. Little is known concerning the effects of common and alternative agronomic practices on nematodes in fields with nematode population densities below threshold levels. Therefore, the objective of this study was to evaluate the effects of the combination of tillage (conventional tillage and no-tillage), irrigation (irrigated and non-irrigated), wheat (Triticum aestivum) residue burning (burned and no burned), …

Analyzing Multigene Stacking And Genome Editing Strategies In Rice, Bhuvan Pathak

Graduate Theses and Dissertations

Crop improvement through biotechnology is an integrated effort, incorporating multiple approaches like integration of genes, editing of native genes, and removal of selection marker genes. Before streamlining the protocols, the efficiency and feasibility of the individual approach and their components must be tested. This study evaluated following approaches: 1) stacking an array of genes into a single locus by site-specific integration via Cre-lox recombination in rice, 2) determining the efficiency of I-SceI and the CCR5-ZFN in the targeted excisions of gene fragments in rice and Arabidopsis, and 3) determining the efficiency of CRISPR/Cas9 in generating targeted mutations for genome editing …

Evaluation Of Topramezone For Use In Rice (Oryza Sativa L.) Production, Matthew Moore

Graduate Theses and Dissertations

The rapid development of resistant weeds, particularly to postemergence-applied herbicides, is a growing concern to rice producers worldwide. Barnyardgrass, one of the most problematic weeds in rice cropping systems, alone has been found to be resistant to four herbicide sites of action (SOA) in certain populations, leaving growers no options for control. This unsettling fact has led to research on other SOAs that have not previously been used in US rice. One SOA that has garnered interest is the 4-hydroxyphenylpyruvate dioxygenase (HPPD)- inhibiting herbicides. The only HPPD-inhibitor that will soon be labeled for use in US rice is benzobicyclon. The …