Digital Commons Network^™

Catalytic Mechanism For The Conversion Of Salicylate Into Catechol By The Flavin-Dependent Monooxygenase Salicylate Hydroxylase, Débora M. A. Costa, Stefanya V. Gómez, Simara S. De Araújo, Mozart S. Pereira, Rosemeire B. Alves, Denize C. Favaro, Alvan C. Hengge, Ronaldo A. P. Nagem, Tiago A. S. Brandão

Chemistry and Biochemistry Faculty Publications

Salicylate hydroxylase (NahG) is a flavin-dependent monooxygenase that catalyzes the decarboxylative hydroxylation of salicylate into catechol in the naphthalene degradation pathway in Pseudomonas putida G7. We explored the mechanism of action of this enzyme in detail using a combination of structural and biophysical methods. NahG shares many structural and mechanistic features with other versatile flavin-dependent monooxygenases, with potential biocatalytic applications. The crystal structure at 2.0 Å resolution for the apo form of NahG adds a new snapshot preceding the FAD binding in flavin-dependent monooxygenases. The k_cat/K_m for the salicylate reaction catalyzed by the holo form is …

Major Grant Awarded To Usu Biological Engineering Professor | College Of Engineering, Utah State University

College of Engineering News

USU biological engineering assistant professor Elizabeth Vargis received a three-year grant from the National Eye Institute for her ongoing research in better understanding why new blood cells form during retinal disease.

Challenges In Columbia River Fisheries Conservation: A Response To Duda Et Al., Brian K. Hand, Courtney G. Flint, Chris A. Frissell, Clint C. Muhlfeld, Shawn P. Devlin, Brian P. Kennedy, Robert L. Crabtree, W. Arthur Mckee, Gordon Luikart, Jack A. Stanford

Sociology, Social Work and Anthropology Faculty Publications

The salmonid fisheries of the Columbia River Basin (CRB) have enormous socioeconomic, cultural, and ecological importance to numerous diverse stakeholders (eg state, federal, tribal, nonprofit), and there are a wide array of opinions and perspectives on how these fisheries should be managed. Although we appreciate Duda et al.'s commentary, it offers only one perspective of many in this context. The objective of our paper (Hand et al. 2018) was to provide justification for “the importance of social–ecological perspectives when communicating conservation values and goals, and the role of independent science in guiding management policy and practice for …

Survey Of Economic Implications Of Maritime And Territorial Disputes, Ronald U. Mendoza, Charles S. Siriban, Tea Jalin Ty

Ateneo School of Government Publications

Drawing on studies on the economics of conflict, this paper reviews the literature on maritime and territorial disputes; and it examines an array of economic implications associated with territorial and maritime disputes. These include adverse effects on certain economic and development outcomes arising from possible armed confrontation, with some of these possibly lingering in the aftermath of conflict. There are also various economic disruptions and costs associated with these disputes, emphasizing how they also affect the livelihoods of resource users in the disputed areas. A clearer understanding of these economic links could help inform and motivate policymakers on mitigating the …

Schoolwide Positive Behavioral Interventions And Supports In An Alternative Education Setting: Examining The Risk And Protective Factors Of Responders And Non-Responders, Amy-Jane Griffiths, Jared T. Izumi, James Alsip, Michael Furlong, Gale M. Morrison

Education Faculty Articles and Research

This research examined the risk and protective factors of responders and nonresponders to a schoolwide implementation of positive behavioral interventions and supports (SW-PBIS) within an alternative school. Students completed self-perception measures of individual, school, community, and home systems. Multivariate analysis of variance indicated a statistically significant difference between responders and nonresponders on the individual and school systems models. Direct logistic regression indicated that within these models, hostility, destructive expression of anger, depression, academic self-concept, attitude to teachers, and attitude to school each made a significant contribution in identifying responders and nonresponders. Findings suggest that factors at the individual and school …

2019 January 28 - Graduate Council Minutes, College Of Graduate And Continuing Studies, East Tennessee State University

Graduate Council Agendas and Minutes

No abstract provided.

Unh Financial Update, Faculty Senate

Faculty Senate Agendas & Minutes

No abstract provided.

3 Researchers Build Interdisciplinary Teams To Tackle Big Issues In Their Field, Haley Herfurth

Browse All News

No abstract provided.

Transplanting Pig Hearts Into Sick Babies May Be A Promising Temporary Treatment Option, Adam Pope

Browse All News

No abstract provided.

Overview Of The Biocreative Vi Precision Medicine Track: Mining Protein Interactions And Mutations For Precision Medicine, Rezarta Islamaj Doğan, Sun Kim, Andrew Chatr-Aryamontri, Chih-Hsuan Wei, Donald C. Comeau, Rui Antunes, Sérgio Matos, Qingyu Chen, Aparna Elangovan, Nagesh C. Panyam, Karin Verspoor, Hongfang Liu, Yanshan Wang, Zhuang Liu, Berna Altınel, Zehra Melce Hüsünbeyi, Arzucan Özgür, Aris Fergadis, Chen-Kai Wang, Hong-Jie Dai, Tung Tran, Ramakanth Kavuluru, Ling Luo, Albert Steppi, Jinfeng Zhang, Jinchan Qu, Zhiyong Lu

Computer Science Faculty Publications

The Precision Medicine Initiative is a multicenter effort aiming at formulating personalized treatments leveraging on individual patient data (clinical, genome sequence and functional genomic data) together with the information in large knowledge bases (KBs) that integrate genome annotation, disease association studies, electronic health records and other data types. The biomedical literature provides a rich foundation for populating these KBs, reporting genetic and molecular interactions that provide the scaffold for the cellular regulatory systems and detailing the influence of genetic variants in these interactions. The goal of BioCreative VI Precision Medicine Track was to extract this particular type of information and …

The Daily Gamecock, Monday, January 28, 2019, University Of South Carolina, Office Of Student Media

January

No abstract provided.

The Rider (2019-01-28), Victor G. Ramirez

The Rider Archives

Vol.4 No.17

Research Progress On The Controlled Release Technology Of Active Compounds From Active Packaging Film, Chen Chenwei, Wang Jiaxi, Yang Fuxin, Xie Jing, Tang Zhipeng

Food and Machinery

The controlled release technology of active compounds released from the active packaging films was reviewed. It was summarized mainly from three aspects, including the control technologies of the release rate for active compounds, its influence factors and release kinetic models. It was aimed to provide a reference for future research on the active packaging film.

Elementary Study On The Construction Of Hplc Characteristic Chromatogramand Spectral-Effect Relationship Of Anthocyanin Extract In Onion, Xiao Ruixi, Wang Xinyue, Chen Huaguo, Zhou Xin

Food and Machinery

A high performance liquid chromatographic (HPLC) method was used to detect anthocyanin extract from onion in 10 regions and the common peaks were identified. The Pearson Correlation Analysis and Grey Relation Aanlysis were used to analyze the correlation between its common peaks, content, antioxidant activity (the ability to scavenge DPPH, ABTS free radicals) and AChE inhibitory activitiy. Result: According to the characte-ristics of HPLC and relative retention time of different batches of onion anthocyanins, and the characteristic chromatograms of onion anthocyanins were established. The chromatograms of anthocyanin extract from onion in 10 different regions were similar to the control fingerprints, …

Rice Bran Polysaccharides Prevent Dss-Induced Colitis Through Mapk Signaling Pathway In Mice, Liu Jing, Guo Ting, Guo Tianyi, Zou Jiawen, Zhou Xiaotian, He Jie, Shi Yuhong, Luo Feijun

Food and Machinery

To assess the anti-inflammatory function and possible molecular mechanism of rice bran polysaccharide (RBP), DSS-induced colitis model was used in ICR mice. Pathological changes and gene expression were analyzed by histopathological analysis, RT-qPCR and Western blotting among of different experiment groups. The results showed that 500 mg/kg/day RBP could significantly improve the health status of mice and reduced colonic inflammation. RBP reduced the disease active index (DAI) score of DSS-induced mice and alleviated the damage of pathological tissue, with the improvement of the inflammation-related biochemical indicators. qRT-PCR analysis showed that RBP could decrease the mRNA expression level of inflammatory factors, …

Effects Of Polyphenols From Areca Seed On Intestinal Microbes In Vitro, Cheng Huan, Wang Yuanliang

Food and Machinery

This study aimed to investigating the effects of the polyphenols from Areca seeds on young men’ s intestinal microbes in vitro through intermittent static anaerobic fermentation technology. The feces of 24 years healthy young men are taken as starter. The substrates of the control group (group C) and the experimental group (group P) with a purity of 74.09% were fermented in vitro at 2, 6, 12 and 24 h. pH value, polyphenol composition, short chain fatty acid (SCFAs) and microorganism index were measured by gas chromatography and high-throughput sequencing. The results showed that the polyphenols of the Areca-nut polyphenols had …

Evaluation Of Aroma Components In Rosa Roxburghii Tratt Juice By Spme-Gc-Ms And Pca, Gu Yuanting, Peng Bangyuan, Ding Zhuhong

Food and Machinery

We used 50/30 μm DVB/CAR/PDMS, 75 μm CAR/PDMS, 65 μm PDMS/DVB, 100 μm PDMS to enrich and concentrate aroma components of Rosa roxburghii Tratt juice for improving the accuracy and reliability of analyzing the aroma components by gas chromatography and comparing the consequence and sensitivity of extracting the aroma components by different fiber coating extractors. The aroma components of Rosa roxburghii Tratt juice were detected by GC-MS. We compared the quantity and content of aroma components by principal component analysis (PCA) and established the evaluation model of aroma quality. The results showed that: esters, alcohols, and terpenes were the main …

Study On Preheating And Ultrasound-Assisted Extraction Of Polyphenols From Corn Silk And Their Antioxidant Activities, Xu Yingyi, Xu Yanxia, Wang Yu, Lin Wei, Wu Hongyan, Meng Xiangwen

Food and Machinery

Ultrasonic-assisted ethanol extraction after heating was used to extract polyphenols from corn silk. On the basis of single factor test, the response surface optimization test was carried out on polyphenols by ultrasonic-assisted ethanol extractions, and the antioxidant activity of the extracted polyphenols was studied. The results showed that the optimum extraction conditions were as follows: the ratio of solid to liquid 1∶22 (g/mL), the volume fraction of ethanol 60%, the ultrasonic time 32 min, and the ultrasonic temperature 60 ℃，the extraction rate of the polyphenol from corn silk was (6.49 ± 0.23)%. The scavenging rates of DPPH，hydroxyl and ABTS free …

Study On Extraction Process Of Polyphenols From Atractylodes Macrocephalakoidz Grown In Qimen And Its Antioxidant And Antibacterial Activities, Hu Changyu, Dai Yi, Wang Yaqun, Lu Weiwei, Wang Weidong, Wu Yongxiang

Food and Machinery

The present study aimed to optimize the process parameters for the surfactant sodium dodecyl sulfonate (SDS)-ultrasonic extraction technology of polyphenols from Atractylodes macrocephala Koidz grown in Qimen (Qi Baizhu). The effects of polyphenols from Qi Baizhu (QBP) on antioxidant and antibacterial activities were explored. The results showed that the optimal process conditions were as follows: SDS concentration 0.47%, extraction temperature 48 ℃, ultrasonic power 150 W, ethanol concentration 64%, extraction time 15 min, and solid-liquid ratio 1∶25 （g/mL）. Under these conditions, the content of QBP was (38.53±0.51) mg/g. QBP exhibited strong DPPH and ABTS free radical scavenging capacities with IC …

Mathematical Model Of Spray Drying Of Whole Egg Powder Based On Yield Optimization, Cheng Fangyuan, Zhou Xueyong, Liu Yanmei, Rajasaba. H.

Food and Machinery

The aim of this experiment is to optimize the spray drying yield of egg liquid. The yield of whole egg powder and the function indexes of protein content, fat content, phospholipid content and water content of egg powder were obtained by orthogonal test under different processing parameters. The Mathematical model was established by Matlab software, which associated the processing parameters with the egg powder yield, protein content and water content. The mathematical models were optimized with the core index of protein yield. The optimum processing conditions for spray drying were: the inlet air temperature 180 ℃, the egg liquid concentration …

Advances On Application Of Predictive Microbiology In Shelf Life Of Aquatic Products, Shen Yong, Mei Jun, Xie Jing

Food and Machinery

Aquatic products is easily deteriorated，and microorganism is the main factor that leads to the deterioration. Predictive microbiology can predict the growth of spoilage organisms in aquatic products by building mathematical models, which help us predict the shelf-life of aquatic products. This paper briefly describes the meaning of predictive microbiology primary model, secondary model and tertiary model, enumerates the applications of predictive microbiology in shelf-life, and discuss the problems of predictive microbiology in the shelf-life prediction, and its application prospect is also proposed.

When Do Psychosocial Explanations Of Psychiatric Problems Increase Stigma? Self-Report And Implicit Evidence, Ryan Thibodeau, Gabrielle L. Amberger

Psychology Faculty/Staff Publications

Background and objectives

Biomedical explanations of psychiatric problems, compared to psychosocial explanations, may amplify psychiatric stigma. One limitation of existing research is the measurement of almost exclusively self-reported stigma. This study evaluated the stigma-related effects of biomedical versus psychosocial explanations of schizophrenia using conventional self-report and two other measurement approaches that may tap more deeply held attitudes.

Methods

One hundred three undergraduates listened to a vignette describing a man with (1) schizophrenia of biomedical origin, (2) schizophrenia of psychosocial origin, or (3) diabetes. They then completed an Implicit Association Test, conventional self-report stigma measures, and projected other measures that captured …

Robot Love, Margaret Ryznar

Seton Hall Law Review

No abstract provided.

Mfd Protects Against Oxidative Stress In Bacillus Subtilis Independently Of Its Canonical Function In Dna Repair, Holly Anne Martin, Katelyne E. Porter, Carmen Vallin, Tatiana Ermi, Natalie Contreras, Mario Pedraza-Reyes, Eduardo Robleto

Life Sciences Faculty Research

Background: Previous reports showed that mutagenesis in nutrient-limiting conditions is dependent on Mfd in Bacillus subtilis. Mfd initiates one type of transcription-coupled repair (TCR); this type of repair is known to target bulky lesions, like those associated with UV exposure. Interestingly, the roles of Mfd in repair of oxidative-promoted DNA damage and regulation of transcription differ. Here, we used a genetic approach to test whether Mfd protected B. subtilis from exposure to two different oxidants. Results: Wild-type cells survived tert-butyl hydroperoxide (t-BHP) exposure significantly better than Mfd-deficient cells. This protective effect was independent of UvrA, a component of the canonical …

Checklist Of Bees (Hymenoptera: Apoidea) From Small Diversified Vegetable Farms In South-Western Montana, Casey M. Delphia, Terry Griswold, Elizabeth G. Reese, Kevin M. O'Neill, Laura A. Burkle

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Background

Over three years (2013-2015), we sampled bees using nets and bowl traps on four diversified vegetable farms in Gallatin County, Montana, USA, as part of a study evaluating the use of wildflower strips for supporting wild bees and crop pollination services on farmlands (Delphia et al. In prep). We document 202 species and morphospecies from 32 genera within five families, of which 25 species represent the first published state records for Montana. This study increases our overall understanding of the distribution of wild bee species associated with agroecosystems of the northern US Rockies, which is important for efforts aimed …

Mcsa Forum Minutes 01/28/2019, Morris Campus Student Association

Morris Campus Student Association

No abstract provided.

Deep Learning Based Analysis Of Histopathological Images Of Breast Cancer, Juanying Xie, Ran Liu, Joseph Luttrell Iv, Chaoyang Zhang

Faculty Publications

Breast cancer is associated with the highest morbidity rates for cancer diagnoses in the world and has become a major public health issue. Early diagnosis can increase the chance of successful treatment and survival. However, it is a very challenging and time-consuming task that relies on the experience of pathologists. The automatic diagnosis of breast cancer by analyzing histopathological images plays a significant role for patients and their prognosis. However, traditional feature extraction methods can only extract some low-level features of images, and prior knowledge is necessary to select useful features, which can be greatly affected by humans. Deep learning …

Tangential Flow Filtration And Scalability In Viral Vector Purification, Eni Sterjanaj, Heather Mall, Rachel Legmann, Jacky Dang

Advancing Manufacture of Cell and Gene Therapies VI [2019]

Pall Biotech has linearly scalable tangential flow filtration technology that can cover processing volume ranges from less than a liter to over 2000 liters. Manual assemblies can be used with Pall TFF filters in scales under 20L and programmable skids are available for larger scales. In this document TFF linear scalability of up to 200 liters is covered starting from a 7L initial volume and maintaining similar pressures, processing times and yields at all scales. Using this scalable technology Pall biotech can help biologics companies go to clinical trials and to commercial scale manufacturing successfully.

Please click Additional Files below …

Little Race Or Gender Bias In An Experiment Of Initial Review Of Nih R01 Grant Proposals, Patrick S. Forscher, William T.L. Cox, Markus Brauer, Patricia G. Devine

Psychological Science Faculty Publications and Presentations

Many granting agencies allow reviewers to know the identity of a proposal’s Principal Investigator (PI), which opens the possibility that reviewers discriminate on the basis of PI race and gender. We investigated this experimentally with 48 NIH R01 grant proposals, representing a broad spectrum of NIH-funded science. We modified PI names to create separate White male, White female, Black male, and Black female versions of each proposal, and 412 scientists each submitted initial reviews for three proposals. We find little to no race or gender bias in initial R01 evaluations, and additionally find that any bias that might have been …

Amino Acid Composition Analysis And Nutritional Value Evaluation Of 17 Walnut Kernels In Yunnan, Li Rui, Lu Bin, Liu Yun, Kan Huan, Hao Jiabo, Mao Yunling

Food and Machinery

Detected the protein content and amino acid content of walnut kernels collected from 17 producing areas in Yunnan, and the amino acid model and nutritional value were comprehensively investigated by amino acid classification, amino acid ratio coefficient method and cluster analysis method. The conclusion indicated that the walnuts derived from each of the various regions were all rich in 17 amino acids (tryptophan was not detected), and the total amount of amino acids was 3.35% to 5.14%, the essential amino acid content was 1.13% to 1.76%, the medicinal amino acid content was 67.47% to 68.63% of the total amino acids, …