Digital Commons Network^™

Spheroid-3d And Monolayer-2d Intestinal Electrochemical Biosensor For Toxicity/Viability Testing: Applications In Drug Screening, Food Safety, And Environmental Pollutant Analysis, Evangelia Flampouri, Shahzad Imar, Kieran O'Connell, Baljit Singh

Articles

The rise of three-dimensional cell culture systems that provide in vivo-like environments for pharmaco-toxicological models has prompted the need for simple and robust viability assays suitable for complex cell architectural structures. This study addresses that challenge with the development of an in vitro enzyme based electrochemical sensor for viability/cytotoxicity assessment of two-dimensional (2D) monolayer and three-dimensional (3D) spheroid culture formats. The biosensor measures the cell viability/toxicity via electrochemical monitoring of the enzymatic activity of nonspecific esterases of viable cells, through the hydrolysis of 1-naphthyl acetate to 1-naphthol. The proposed sensor demonstrated strong correlation (r = 0.979) with viable …

Electrochemical Enzyme-Linked Immunosorbent Assay (E-Elisa) For Parasitic Nematode: Ostertagia Ostertagi (Brown Stomach Worm) Infections In Dairy Cattle, Baljit Singh, Evangelia Flampouri, Eithne Dempsey

Articles

A sensitive electrochemical immunoassay (e-ELISA) has been developed for the detection of the gastrointestinal parasitic nematode Ostertagia ostertagi (brown stomach worm) in infected and control serum samples. An antigen-indirect immunoassay format was employed to detect the presence of O. ostertagi antibodies, coupled with an anti-species monoclonal horseradish peroxidase (HRP) conjugate. ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) and TMB (3,3',5,5'-tetramethylbenzidine/hydrogen peroxide) were investigated as both chromogenic visualising reagents for optical ELISA and electroactive substrates for electrochemical ELISA in the HRP catalysed oxidation reaction. Coulometry was applied for the detection of O. ostertagi antibodies (via TMB electrochemistry) and compared with the commercial optical ELISA (ABTS …

I Was The Only One In The Building. Lived Experiences Of Black School Counselors Post-Brown V. Board Of Education In Predominantly White Schools, Yevonne A. Jones

All-Inclusive List of Electronic Theses and Dissertations

The purpose of this study was to explore the lived experiences of Black school counselors post- Brown v. Board of Education (1954) decision and their integration into predominantly White public schools. A literature review was conducted which included the history of school counseling, Brown v. Board of Education , and its impact on the displacement of Black educators. The literature also explored the experiences of Black teachers and students post- Brown and current demographic data of school counselors. A qualitative study was conducted to capture the lived experiences of participants using multiple case studies and narrative life stories. Four Black …

A Gis-Based Modeling Approach To High-Resolution Anthropogenic Heat Flux And Building Energy Demand In Los Angeles, Yuanfan Zheng

All-Inclusive List of Electronic Theses and Dissertations

Anthropogenic heat flux (Q f ) originates from energy consumption in buildings, industrial plants, vehicle exhaust, and human metabolism. Q f is an important component of the urban Surface Energy Balance (SEB) system and a key to understanding many urban environmental issues. Climate change affects building energy consumption in many ways, and building energy consumption is the largest contributor to Q f in many cities. One of these contributions comprises changes in heating and cooling demands in buildings. The increase in annual energy use in cities results in more carbon emissions and constitutes a great challenge to urban sustainability because …

How Exemplary Teachers Promote Scientific Reasoning And Higher Order Thinking In Primary Science, Barbara Kim Sherriff

Theses: Doctorates and Masters

There is an emerging interest in the development of STEM capabilities to drive Australia’s future economy and workforce. As a consequence, the focus on the teaching of higher order thinking and scientific reasoning has intensified. Despite these efforts, Australia’s level of achievement on international benchmarking tests has not improved.

The aim of this PhD research was to investigate how exemplary teachers develop higher order thinking and scientific reasoning in primary science. The study drew on video data from the EQUALPRIME international research project, which explored quality primary science education in different cultures (ARC Discovery Project DP110101500).

This qualitative research examined …

Modelling Of The Radiant Heat Flux And Rate Of Spread Of Wildfire Within The Urban Environment, Greg Penney, Steven Richardson

Research outputs 2014 to 2021

One approach to increase community resilience to wildfire impacts is the enhancement of residential construction standards in an effort to provide protective shelters for families within their own homes. Current wildfire models reviewed in this study assume fire growth is unrestricted by vegetation fuel bed geometry; the head fire has attained a quasi-steady rate of spread; and the shielding effects of urban development are ignored. As a result, radiant heat flux may be significantly overestimated for small vegetation fires in road reserves, urban parklands, and similar scenarios. This paper proposes two new models to address this issue, and utilises two …

Population Data Centre Profile - The Western Australian Data Linkage Branch, Steve Hodges, Tom Eitelhuber, Alexandra Merchant, Janine Alan

Research outputs 2014 to 2021

Established in 1995, the Western Australian Data Linkage Branch (DLB) is Australia’s longest running data linkage agency. The Western Australian Data Linkage System (WADLS) employs an enduring linkage model spanning over 60 data collections supported by internally developed and supported software and IT infrastructure. DLB has delivered, and continues to deliver, a range of significant data linkage innovations, many of which have been adopted elsewhere. A current restructure within the Western Australian Department of Health (which we will refer to as the Department of Health) will provide an improved funding model geared toward addressing issues with staff retention, capacity and …

Proof-Of-Familiarity: A Privacy-Preserved Blockchain Scheme For Collaborative Medical Decision-Making, Jinhong Yang, Md Mehedi Hassan Hasan Onik, Nam-Yong Lee, Mohiuddin Ahmed, Chul-Soo Kim

Research outputs 2014 to 2021

The current healthcare sector is facing difficulty in satisfying the growing issues, expenses, and heavy regulation of quality treatment. Surely, electronic medical records (EMRs) and protected health information (PHI) are highly sensitive, personally identifiable information (PII). However, the sharing of EMRs, enhances overall treatment quality. A distributed ledger (blockchain) technology, embedded with privacy and security by architecture, provides a transparent application developing platform. Privacy, security, and lack of confidence among stakeholders are the main downsides of extensive medical collaboration. This study, therefore, utilizes the transparency, security, and efficiency of blockchain technology to establish a collaborative medical decision-making scheme. This study …

Privacy-Preserving Attribute-Based Keyword Search In Shared Multi-Owner Setting, Yibin Miao, Ximeng Liu, Robert H. Deng, Robert H. Deng, Jjguo Li, Hongwei Li, Jianfeng Ma

Research Collection Yong Pung How School Of Law

Ciphertext-Policy Attribute-Based Keyword Search (CP-ABKS) facilitates search queries and supports fine-grained access control over encrypted data in the cloud. However, prior CP-ABKS schemes were designed to support unshared multi-owner setting, and cannot be directly applied in the shared multi-owner setting (where each record is accredited by a fixed number of data owners), without incurring high computational and storage costs. In addition, due to privacy concerns on access policies, most existing schemes are vulnerable to off-line keyword-guessing attacks if the keyword space is of polynomial size. Furthermore, it is difficult to identify malicious users who leak the secret keys when more …

A Network Analysis Of The Singapore Court Of Appeal’S Citations To Precedent, Jerrold Tsin Howe Soh

Research Collection Yong Pung How School Of Law

This article presents findings from an empirical network analysis of citation practices in Singapore's highest court.A network of all 987 reported Court of Appeal judgments handed down from 2000 to 2017 is constructed. Networkcentrality algorithms are used to rank judgments by centrality. Judgments on contract law, particularly on contractual interpretation and terms, emerge as the most central. Based on this, this article argues that more attention can be paid to interpretation per se as a legal skill. More generally, this article establishes a framework for applying network analysis to Singapore jurisprudence on a larger scale.

A Hardware-Deployable Neuromorphic Solution For Encoding And Classification Of Electronic Nose Data, Anup Vanarse, Alexander Rassau, Peter Van Der Made

Research outputs 2014 to 2021

In several application domains, electronic nose systems employing conventional data processing approaches incur substantial power and computational costs and limitations, such as significant latency and poor accuracy for classification. Recent developments in spike-based bio-inspired approaches have delivered solutions for the highly accurate classification of multivariate sensor data with minimized computational and power requirements. Although these methods have addressed issues related to efficient data processing and classification accuracy, other areas, such as reducing the processing latency to support real-time application and deploying spike-based solutions on supported hardware, have yet to be studied in detail. Through this investigation, we proposed a spiking …

Design Of A Hybrid Measure For Image Similarity: A Statistical, Algebraic, And Information-Theoretic Approach, Mohammed Abdulameer Aljanabi, Zahir M. Hussain, Noor Abd Alrazak Shnain, Song Feng Lu

Research outputs 2014 to 2021

Image similarity or distortion assessment is fundamental to a wide range of applications throughout the field of image processing and computer vision. Many image similarity measures have been proposed to treat specific types of image distortions. Most of these measures are based on statistical approaches, such as the classic SSIM. In this paper, we present a different approach by interpolating the information theory with the statistic, because the information theory has a high capability to predict the relationship among image intensity values. Our unique hybrid approach incorporates information theory (Shannon entropy) with a statistic (SSIM), as well as a distinctive …

Validity Of The Velocomp Powerpod Compared With The Verve Cycling Infocrank Power Meter, Paul F. J. Merkes, Paolo Menaspà, Chris R. Abbiss

Research outputs 2014 to 2021

Purpose:

To determine the validity of the Velocomp PowerPod power meter in comparison with the Verve Cycling InfoCrank power meter.

Methods:

This research involved 2 separate studies. In study 1, 12 recreational male road cyclists completed 7 maximal cycling efforts of a known duration (2 times 5 s and 15, 30, 60, 240, and 600 s). In study 2, 4 elite male road cyclists completed 13 outdoor cycling sessions. In both studies, power output of cyclists was continuously measured using both the PowerPod and InfoCrank power meters. Maximal mean power output was calculated for durations of 1, 5, …

Projected Sea Level Rise And The Conservation Ecology Of The Micronesian Megapode (Megapodius Laperouse Senex) In Palau, Micronesia, Paul M. Radley

Theses: Doctorates and Masters

Climate change has been a subject of numerous studies. While findings suggest that most biological taxa will be affected by its manifestations, aspects of a species life history may increase its susceptibility to climate change. Given their reliance on environmental sources of heat to incubate their eggs, I examined the vulnerability to climate change of the avian family Megapodiidae. I also assessed habitat use, susceptibility to sea level rise, and the effect of introduced rats and tourist presence, as added stressors to climate change, on the Micronesian Megapode (Megapodius

laperouse senex) in Palau.

Based on available literature, I …

Chemical Composition And Toxicity Of Emissions From Burning Five Vegetation Types Of Western Australia Under Experimental Combustion Conditions, T T Trang Dong

Theses: Doctorates and Masters

This study investigated the emission factors (EFs) for inorganic gases (CO2, CO, SO2, NO and NO2), carbonyls (formaldehyde, acetaldehyde, acetone, propionaldehyde, butyraldehyde and benzaldehyde), volatile organic compounds (VOCs) and particulate matter (PM2.5 and PM10) from laboratory-based fires of vegetation from five typical vegetation types of Western Australia. Species burnt were three grasslands (Spinifex represented by Triodia basedowii, Kimberley grass represented by Sehima nervosum and Heteropogon contortus, and an invasive grass represented by Ehrharta calycina (Veldt grass)), Banksia woodland and Jarrah forest under different combustion conditions. Chemical composition (water-soluble metals and polycyclic aromatic hydrocarbons – PAHs) and in vitro toxicity …

Anomaly Detection In Bacnet/Ip Managed Building Automation Systems, Matthew Peacock

Theses: Doctorates and Masters

Building Automation Systems (BAS) are a collection of devices and software which manage the operation of building services. The BAS market is expected to be a $19.25 billion USD industry by 2023, as a core feature of both the Internet of Things and Smart City technologies. However, securing these systems from cyber security threats is an emerging research area. Since initial deployment, BAS have evolved from isolated standalone networks to heterogeneous, interconnected networks allowing external connectivity through the Internet. The most prominent BAS protocol is BACnet/IP, which is estimated to hold 54.6% of world market share. BACnet/IP security features are …

An Investigation Of Secondary Student Perceptions Of Fairness About Assessment Processes Implemented By Their Teachers, Tania Blazevic

Theses: Doctorates and Masters

This research investigated the effects of secondary school teachers’ assessment practices on students’ perceptions of fairness in Croatia. It focused on the extent to which teachers implemented consistent assessment practices and made judgements of student achievements, and how this affected students’ perceptions.

The need for assessment influences its creation and implementation, which in turn influences the outcomes. Teachers’ assessment of students is an ongoing practice in schools in Croatia, both officially and unofficially, and questions about consistent application have persisted. This research was motivated by a confrontation with a disgruntled student and set in motion an examination of teachers’ application …

Biodiversity Offsets In Eia: Getting The Timing Right, Marlene De Witt, Jenny Pope, Francois Retief, Alan Bond, Angus Morrison-Saunders, Carli Steenkamp

Research outputs 2014 to 2021

Major developments can result in significant impacts on biodiversity, which the mandated process of environmental impact assessment (EIA) aims to mitigate. There has been a recent move towards the application of biodiversity offsets as a last-resort, compensatory measure when options at the earlier stages in the mitigation hierarchy of avoidance, minimisation and restoration have been exhausted. Guidance on biodiversity offset planning available in different jurisdictions, however, demonstrates a lack of consensus about when biodiversity offsets should be formally introduced into the EIA process, and previous research has highlighted the perceived risks associated with commencing detailed offset planning too early as …

Thermal Performance Of An Evacuated Tube Heat Pipe Solar Water Heating System In Cold Season, Abdellah Sharifian, Mehdi Khiadani, Ataollah Nosrati

Research outputs 2014 to 2021

This study evaluates the performance of a heat pipe solar water heating system to meet a real residential hot water consumption pattern theoretically and experimentally under non-ideal climatic conditions during a cold day in Perth, Western Australia. A mathematical model was developed and used to calculate the optimum number of glass tubes of the heat pipe solar collector. Based on the obtained data, an experimental rig with 25 glass tubes was designed, built, and tested as the temperature changes after 25 tubes reached the insignificant value of 0.6%. The results showed that hot water extraction had significant impact on the …

Time-To-Event Analysis For Sports Injury Research Part 1: Time-Varying Exposures, Rasmus Oestergaard Nielsen, Michael Lejbach Bertelsen, Daniel Ramskov, Merete Møller, Adam Hulme, Daniel Theisen, Caroline F. Finch, Lauren Victoria Fortington, Mohammad Ali Mansournia, Erik Thorlund Parner

Research outputs 2014 to 2021

BACKGROUND: ‘How much change in training load is too much before injury is sustained, among different athletes?’ is a key question in sports medicine and sports science. To address this question the investigator/practitioner must analyse exposure variables that change over time, such as change in training load. Very few studies have included time-varying exposures (eg, training load) and time-varying effect-measure modifiers (eg, previous injury, biomechanics, sleep/stress) when studying sports injury aetiology.

AIM: To discuss advanced statistical methods suitable for the complex analysis of time-varying exposures such as changes in training load and injury-related outcomes.

CONTENT: Time-varying exposures and time-varying effect-measure …

Strategies To Improve The Thermal Performance Of Heat Pipe Solar Collectors In Solar Systems: A Review, Abdellah Sharifian, Mehdi Khiadani, Ataollah Nosrati

Research outputs 2014 to 2021

Invention of evacuated tube heat pipe solar collectors (HPSCs) was a huge step forward towards resolving the challenges of conventional solar systems due to their unique features and advantages. This has led to their utilization in a wide range of solar applications surpassing other conventional collectors. However, relatively low thermal efficiency of heat pipe solar (HPS) systems is still the major challenge of solar industry evidenced by numerous studies conducted mainly during the last decade to improve their efficiency. To date, several review papers have been published summarizing studies relevant to utilization of HPSCs in various thermal applications. However, to …

Understanding Visitor Expectations For Responsible Tourism In An Iconic National Park: Differences Between Local And International Visitors, Angus Morrison-Saunders, Michael Hughes, Jenny Pope, Anneli Douglas, Jan-Albert Wessels

Research outputs 2014 to 2021

A survey on expectations for responsible tourism was administered to 201 visitors to the iconic Kruger National Park, comprising 55% South African nationals and 45% international tourists. Using Likert-scale questions, respondents were invited to indicate the level of importance they attached to 38 aspects of responsible tourism. Space was also provided for open comments. Conservation of biodiversity and geological features were rated very highly by both groups while aspects related to the local community, though considered important overall, were ranked lowest overall, with international tourists rating this category as more important than South Africans. Aspects related to traffic and access …

Doppler Radar-Based Non-Contact Health Monitoring For Obstructive Sleep Apnea Diagnosis: A Comprehensive Review, Vinh Phuc Tran, Adel Ali Al-Jumaily, Syed Mohammed Shamsul Islam

Research outputs 2014 to 2021

Today’s rapid growth of elderly populations and aging problems coupled with the prevalence of obstructive sleep apnea (OSA) and other health related issues have affected many aspects of society. This has led to high demands for a more robust healthcare monitoring, diagnosing and treatments facilities. In particular to Sleep Medicine, sleep has a key role to play in both physical and mental health. The quality and duration of sleep have a direct and significant impact on people’s learning, memory, metabolism, weight, safety, mood, cardio-vascular health, diseases, and immune system function. The gold-standard for OSA diagnosis is the overnight sleep monitoring …

Neuromeasure: A Software Package For Quantification Of Cortical Motor Maps Using Frameless Stereotaxic Transcranial Magnetic Stimulation, Michael B. Gerber, Alasdair C. Mclean, Samuel J. Stephen, Alex G. Chalco, Usman M. Arshad, Gary W. Thickbroom, Josh Silverstein, K. Zoe Tsagaris, Amy Kuceyeski, Kathleen Friel, Taiza E. G. Santos, Dylan J. Edwards

Research outputs 2014 to 2021

The recent enhanced sophistication of non-invasive mapping of the human motor cortex using MRI-guided Transcranial Magnetic Stimulation (TMS) techniques, has not been matched by refinement of methods for generating maps from motor evoked potential (MEP) data, or in quantifying map features. This is despite continued interest in understanding cortical reorganization for natural adaptive processes such as skill learning, or in the case of motor recovery, such as after lesion affecting the corticospinal system. With the observation that TMS-MEP map calculation and quantification methods vary, and that no readily available commercial or free software exists, we sought to establish and make …

A Note On Science, Legal Research And Artificial Intelligence, Sean Goltz, Giulia Dondoli

Research outputs 2014 to 2021

This paper discusses the principles of scientific research and in turn review legal research that was done using Artificial Intelligence arguing that it is the tools (Artificial Intelligence) that take center stage while the meaning (legal research) is left back stage. In turn, this kind of research does not adhere to the fundamentals of scientific research nor comply with scientific and industry ethical codes.

Fractional Anisotropy Of Thalamic Nuclei Is Associated With Verticality Misperception After Extra-Thalamic Stroke, Taiza E. G. Santos, Jussara A. O. Baggio, Carlo Rondinoni, Laura Machado, Karina T. Weber, Luiz H. Stefano, Antonio C. Santos, Octavio M. Pontes-Neto, Joao P. Leite, Dylan J. Edwards

Research outputs 2014 to 2021

Verticality misperception after stroke is a frequent neurological deficit that leads to postural imbalance and a higher risk of falls. The posterior thalamic nuclei are described to be involved with verticality perception, but it is unknown if extra-thalamic lesions can have the same effect via diaschisis and degeneration of thalamic nuclei. We investigated the relationship between thalamic fractional anisotropy (FA, a proxy of structural integrity), and verticality perception, in patients after stroke with diverse encephalic extra-thalamic lesions. We included 11 first time post-stroke patients with extra-thalamic primary lesions, and compared their region-based FA to a group of 25 age-matched healthy …

Acute Effects Of Fine Particulate Matter (Pm2.5) On Hospital Admissions For Cardiovascular Disease In Beijing, China: A Time-Series Study, Endawoke Amsalu, Tianqi Wang, Haibin Li, Yue Liu, Anxin Wang, Xiangtong Liu, Lixin Tao, Yanxia Luo, Feng Zhang, Xinghua Yang, Xia Li, Wei Wang, Xiuhua Guo

Research outputs 2014 to 2021

Background

Air pollution and cardiovascular disease are increasing problems in China. However, the short-term association between fine particulate matter (PM2.5) and cardiovascular disease (CVD) is not well documented. The purpose of this study is to estimate the short-term effects of PM2.5 on CVD admissions in Beijing, China.

Methods

In total, 460,938 electronic hospitalization summary reports for CVD between 2013 and 2017 were obtained. A generalized additive model using a quasi-Poisson distribution was used to investigate the association between exposure to PM2.5 and hospitalizations for total and cause-specific CVD, including coronary heart disease (CHD), atrial fibrillation (AF), and heart failure (HF) …

Calculation Of Oxygen Diffusion Coefficients In Oxide Films Formed On Low-Temperature Annealed Zr Alloys And Their Related Corrosion Behavior, Lina Zhang, Liang-Yu Chen, Cuihua Zhao, Yujing Liu, Lai-Chang Zhang

Research outputs 2014 to 2021

The growth of oxide film, which results from the inward oxygen diffusion from a corrosive environment, is a critical consideration for the corrosion resistance of zirconium alloys. This work calculates the oxygen diffusion coefficients in the oxide films formed on zirconium alloys annealed at 400~500 °C and investigates the related corrosion behavior. The annealed samples have a close size for the second-phase particles but a distinctive hardness, indicating the difference in substrate conditions. The weight gain of all samples highly follows parabolic laws. The weight gain of the sample annealed at 400 °C has the fastest increase rate at the …

Calculation Of Critical Water Flow Rates For Wildfire Suppression, Greg Penney, Daryoush Habibi, Marcus Cattani, Murray Carter

Research outputs 2014 to 2021

Predicting water suppression requirements and its impacts on firefighting strategies and logistics within the urban environment has been the subject of many previous studies, however the same level of research has yet to be applied in the realm of wildfire suppression. To work towards addressing this knowledge gap, this paper provides guidance for Incident Controllers in relation to critical water flow rates required to extinguish large wildfire across a wide range of forest fuel loads, fire weather and active fire front depths. This is achieved through mathematical empirical analysis of water flow rates required for head fire suppression during 540 …

Simulating Rewetting Events In Intermittent Rivers And Ephemeral Streams: A Global Analysis Of Leached Nutrients And Organic Matter, Oleksandra Shumilova, Dominik Zak, Thibault Datry, Daniel Von Schiller, Roland Corti, Arnaud Foulquier, Biel Obrador, Klement Tockner, Daniel C. Allan, Florian Altermatt, María Isabel Arce, Shai Arnon, Damien Banas, Andy Banegas‐Medina, Erin Beller, Melanie L. Blanchette, Juan F. Blanco‐Libreros, Joanna Blessing, Iola Gonçalves Boëchat, Kate Boersma, Michael T. Bogan, Núria Bonada, Nick R. Bond, Kate Brintrup, Andreas Bruder, Ryan Burrows, Tommaso Cancellario, Stephanie M. Carlson, Sophie Cauvy‐Fraunié, Núria Cid, Michael Danger, Bianca De Freitas Terra, Anna Maria De Girolamo, Ruben Del Campo, Fiona Dyer, Arturo Elosegi, Emile Faye, Catherine Febria, Ricardo Figueroa, Brian Four, Mark O. Gessner, Pierre Gnohossou, Rosa Gómez Cerezo, Lluís Gomez‐Gener, Manuel A.S. Graça, Simone Guareschi, Björn Gücker, Jason L. Hwan, Skhumbuzo Kubheka, Simone Daniela Langhans, Catherine Leigh, Chelsea J. Little, Stefan Lorenz, Jonathan Marshall, Angus Mcintosh, Clara Mendoza‐Lera, Elisabeth Irmgard Meyer, Marko Miliša, Musa C. Mlambo, Marcos Moleón, Peter Negus, Dev Niyogi, Athina Papatheodoulou, Isabel Pardo, Petr Paril, Vladimir Pešić, Pablo Rodriguez‐Lozano, Robert J. Rolls, Maria Mar Sanchez‐Montoya, Ana Savić, Alisha Steward, Rachel Stubbington, Amina Taleb, Ross Vander Vorste, Nathan Waltham, Annamaria Zoppini, Christiane Zarfl

Research outputs 2014 to 2021

Climate change and human pressures are changing the global distribution and the extent of intermittent rivers and ephemeral streams (IRES), which comprise half of the global river network area. IRES are characterized by periods of flow cessation, during which channel substrates accumulate and undergo physico‐chemical changes (preconditioning), and periods of flow resumption, when these substrates are rewetted and release pulses of dissolved nutrients and organic matter (OM). However, there are no estimates of the amounts and quality of leached substances, nor is there information on the underlying environmental constraints operating at the global scale. We experimentally simulated, under standard laboratory …