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Precision Medicine And Tuberculosis*, Somchai Bovornkitti

Asian Medical Journal and Alternative Medicine

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Development And Evaluation Of Vaccine Candidates For Senecavirus A, Bishwas Sharma

Electronic Theses and Dissertations

Senecavirus A (SVA) has been associated with several outbreaks of vesicular disease (VD) since 2014 in major swine-producing countries around the world. The virus causes VD that is clinically indistinguishable from foot-and-mouth disease (FMD) in pigs. Currently there are no commercial vaccines available for SVA. Here, we developed a live attenuated SVA strain by mutating a wild type SVA strain SD15-26 (wt SVA). The live attenuated virus, rSVA mSacII, replicated less efficiently in cell cultures in vitro and expressed lower amounts of VP1 and VP2 capsid proteins than wt SVA. Most importantly, the rSVA mSacII virus failed to induce clinical …

Senecavirus A: Pathogenicity And Interactions With Host Cell Death Pathway, Maureen Hoch Vieira Fernandes

Electronic Theses and Dissertations

Senecavirus A (SVA) is a positive sense single-strand RNA virus of the genus Senecavirus, family Picornaviridae. Senecavirus A is an emerging picornavirus of swine, which causes vesicular disease (VD) in pigs that is clinically indistinguishable from footand- mouth disease and other high consequence VDs of swine. There is evidence that SVA has been circulating in the US swine population since the late eighty’s, however, recently the virus emerged worldwide, and it has been associated with several VD outbreaks in pigs in many of the major swine producing countries. Despite the recent surge in the number of cases of …

Greater Sage-Grouse Survival, Breeding Ecology, Resource Selection, And West Nile Virus Prevalence On The Eastern Fringe Of Their Range, Lindsey Anne Parsons

Electronic Theses and Dissertations

The greater sage-grouse (Centrocercus urophasianus) is a sagebrush (Artemisia spp.) obligate species that has experienced population declines over the past several decades. Sage-grouse are a species of conservation concern throughout the Intermountain West and are considered a species of greatest conservation need in South Dakota. Numerous studies have documented drivers of demographic performance at the core of their distribution; however, relatively few studies have examined sage-grouse inhabiting the eastern extent of their range, in South Dakota. We sought to examine sage-grouse space use in multiple seasons, estimate survival, and determine factors affecting nest success in South Dakota …

Alternative Treatment Options For Controlling Anthelmintic-Resistant Haemonchus Contortus Populations In South Dakota Sheep Herds, Adam Sarah

Electronic Theses and Dissertations

Haemonchus contortus is a common economically important gastrointestinal nematode that obliges the survival and productivity of small ruminants, especially sheep and goats. Anthelmintic resistance is a primary challenge in ruminant health management programs in preventing and controlling the H. contortus populations; therefore, there is a great necessity in finding and developing natural plant products for use as alternatives to commercial anthelmintics for controlling H. contortus in pastured sheep and goats. This dissertation has three major aims conducted in three separate studies: the first in vivo one was to evaluate the extended effect of a springtime, combined-treatment with 3 anthelmintics having …

Performance Differences Between Two Groups Of Commercial Pigs Following Experimental Infection With Prrs Virus 1-7-4, Erin Little

Electronic Theses and Dissertations

Porcine reproductive and respiratory syndrome virus (PRRSV) is the costliest swine disease in North America. Vaccines and management alone have not been effective in controlling this disease. Genetic selection for resilience may be a complimentary approach for controlling PRRSV. The objective of this study was to estimate performance differences between two groups of pigs from the same commercial line following infection with PRRSV 1-7-4: 1) pigs sired by boars selected based on a standard index (TN-S), which emphasized feed efficiency and carcass quality; and 2) pigs sired by boars selected based on an experimental index (TN-E), which emphasized feed intake, …

Rumen Microbial Culture Library And In Vitro Analysis Of Selected Bacterial Species On Colonization Resistance Against Bovine Enteric Pathogens, Maria Antony

Electronic Theses and Dissertations

The anaerobic habitat of the four chambers in ruminant’s foregut plays a vital role in the fermentation of the food by the active involvement of the residing microbial community. The cultivability and the potential role of rumen bacteria in the field of livestock production, host health and immunity are important to study. The developed anaerobic culturing techniques enable us to culture and screen different rumen bacteria to study the anti-pathogenic effects. Whole genome sequencing and analysis help us to understand the genome level characteristics of the rumen bacterial species individually. Since the majority of the rumen biosphere is still not …

Development Of A Novel Nanotechnology-Based Vaccine Adjuvant And Delivery System For Systemic And Mucosal Immunity, Mohammed Ali Bakkari

Electronic Theses and Dissertations

Many serious infections for which there are no existing vaccines, enter by a mucosal route such as HIV, influenza, and tuberculosis, etc. Therefore, designing safe and effective mucosal vaccines that elicit robust immune response at mucosal sites is still a major challenge without a safe mucosal adjuvant. Previously, our laboratory had discovered inulin acetate as a novel toll-like-receptor-4 agonist (adjuvant). The study in this dissertation explored the application of nanoparticles prepared with inulin acetate (InAc-NPs) as a vaccine adjuvant and delivery system for enhancing mucosal immunity. The rationale behind selecting InAc-NPs is their proven ability to activate strong systemic immunity …

Cropping Sequence Affects Subsequent Soybean Yield, Soil Microbiome And Soil Health, Izzet Bulbul

Electronic Theses and Dissertations

Rotation is an important cultural practice that farmers use to manage crop growth and diseases. Diversified crop rotations directly affect plant residue inputs that may enrich specific groups of microbes that form beneficial associations with the following crop. At two field sites, yield differences were observed in soybeans planted after the following four long-term (14 year) crop sequences: continuous corn (CC), corn (Zea mays)/corn/soybean (Glycine max) (CCS), corn/soybean/corn (CSC), soybean/corn/soybean (SCS). Soybean yields were in decreasing order, CC>CCS>CSC>SCS, and the yield differences could not be explained by soil chemical and physical properties previously …

Going Rogue: Mobile Research Applications And The Right To Privacy, Stacey A. Tovino

Faculty Articles

This Article investigates whether nonsectoral state laws may serve as a viable source of privacy and security standards for mobile health research participants and other health data subjects until new federal laws are created or enforced. In particular, this Article (1) catalogues and analyzes the nonsectoral data privacy, security, and breach notification statutes of all fifty states and the District of Columbia; (2) applies these statutes to mobile-app-mediated health research conducted by independent scientists, citizen scientists, and patient researchers; and (3) proposes substantive amendments to state law that could help protect the privacy and security of all health data subjects, …

Impact Of Availability Of Telehealth Programs On Documented Hiv Viral Suppression: A Cluster-Randomized Program Evaluation In The Veterans Health Administration, Michael E. Ohl, Kelly Richardson, Maria C. Rodriguez-Barradas, Roger Bedimo, Vincent Marconi, Jamie P. Morano, Michael P. Jones, Mary Vaughan-Sarrazin

Internal Medicine Faculty Publications

Background: Telehealth may improve care for people with HIV who live far from HIV specialty clinics. We conducted a cluster-randomized evaluation to determine the impact of availability of HIV telehealth programs on documented viral suppression in Veterans Administration clinics. Methods: In 2015–2016, people who previously traveled to HIV specialty clinics were offered telehealth visits in nearby primary care clinics. Patients were cluster-randomized to immediate telehealth availability (n = 925 patients in service areas of 13 primary care clinics offering telehealth) or availability 1 year later (n = 745 patients in 12 clinics). Measures during the evaluation year included telehealth use …

Distinct Effects Of Acute Versus Chronic Corticosterone Exposure On Zebra Finch Responses To West Nile Virus, Lynn B. Martin, Meredith E. Kernbach, Thomas R. Unnasch

Internal Medicine Faculty Publications

Wild animals are exposed to both short- (acute) and long-term (chronic) stressors. The glucocorticoid hormones, such as corticosterone (CORT), facilitate coping with such stressors, but these hormones can have quite distinct effects contingent on the duration of their elevation. Previously, we found that experimental elevation of CORT for 2 days (via implantation) affected zebra finch (Taeniopygia guttata) responses to West Nile virus (WNV). CORT-elevated birds had higher viremia for at least 2 days longer than controls, and West Nile virus (WNV)-associated mortality occurred only in CORT-elevated birds. Here, we queried how acute elevations of CORT, via injection an …

A Case Series Of Elizabethkingia Meningosepticum Bacteremia In The Cancer Population, Dae Hyun Lee, Ishita Mehra, Sanjay Chandrasekhar, Abu-Sayeef Mirza, Rahul Shenoy, Annie Topham, Sowmya Nanjappa, John Greene

Internal Medicine Faculty Publications

Background :Elizabethkingia meningosepticum (E. meningosepticum) is a ubiquitous microorganism previously known as Chryseobacterium meningosepticum. It is emerging as a pathogen responsible for bacteremia in immunocompromised patients such as cancer patients especially those with a history of prolonged hospital stay and frequent instrumentations. Methods: A retrospective chart review of all cases over 10 years in Moffitt Cancer Center showed a total of three patients with E. meningosepticum infection. Results: First patient (history of multiple myeloma) underwent endoscopy complicated by aspiration pneumonia and blood culture positive for E. meningosepticum infection. He was treated with ciprofloxacin, cefoxitin, minocycline and metronidazole and was …

Outcomes Of Extended-Spectrum Β-Lactamase-Producing Escherichia Coli Bloodstream Infection In Neutropenic Patients With Hematological Malignancies, Sadaf Aslam, James Denham, John Greene

Internal Medicine Faculty Publications

Background: Infections with extended-spectrum β-lactamase (ESBL) producing Enterobacteriaceae is an emerging problem leading to poor clinical outcomes and increased mortality. The purpose of this study was to determine the prevalence, risk factors and outcomes of ESBL-producing E. coli (EC) in bloodstream infections (BSIs) of neutropenic patients with hematological malignancies and compare the difference with Non-ESBL producing EC.

Methods: Through an IRB approved protocol, a retrospective cohort study was conducted at the H. Lee Moffitt Cancer Center from January, 2007 till October, 2017. Of the 310 records, who had +ive blood cultures for E. Coli, a total of 63 neutropenic patients …

The Impact Of Recent Incarceration On Post-Release Hiv Linkage To Care And Risk-Taking Behaviors In The Setting Of Enhanced Linkage To Care Policies In Florida, Greg Matthew E Teo, Suraj Nagaraj, Nisha Sunku, Sadaf Aslam, Rahul Mhaskar, Liwei Chen, Zhi Zhou, Robert L. Cook, Jamie P. Morano, Charurut Somboonwit

Internal Medicine Faculty Publications

Background: The United States has the largest incarcerated population in the world with 6.61 million adults in 2016.1 While incarceration is a known risk factor for difficulties in linkage to care2–3 and adverse health outcomes4–6, little is published on post-release incarcerated persons living with HIV (PLWH) in Florida.

Methods: Data were acquired from the Florida Cohort, an ongoing, longitudinal, cross-sectional study of PLWH recruited across HIV clinics in the state of Florida, from 2014 to 2018. Chi-square and multiple regression analyses correlated recent incarceration (within last 12 months) with demographics, HIV care adherence, perceived barriers to care, and self-reported high-risk …

Pyoderma Gangrenosum Following Pacemaker Implantation: A Case Report And Review Of Literature, Noura Ayoubi, Zaydi Javeed, Raymond Cutro, Brooke T. Baldwin

Internal Medicine Faculty Publications

Pyoderma gangrenosum (PG) is a rare neutrophilic dermatosis characterized by noninfectious, inflammatory, ulcerating lesions. Pathergy can be seen in these patients, whereby minor trauma or surgery can result in the development of PG ulcerations. Here, we present a case of PG following pacemaker implantation. A 76-year-old male with a history of rheumatoid arthritis presented to the cardiology team with symptomatic bradycardia. Indications for implantation were met, and the procedure was performed in a routine fashion. The patient returned to clinic for follow-up four days later, complaining of pain at the incision site, coupled with erythema and purulent drainage. Consultations with …

Infectious Complications In Adult Leukemic Patients With Prolonged Neutropenia Undergoing Induction Chemotherapy, Vidhya Sabapathy, Olga Klinkova, Joseph Balbona, John Greene

Internal Medicine Faculty Publications

Background: Induction chemotherapy in patients with the diagnosis of acute leukemia is associated with a high incidence of infectious complications. While prior studies provide information regarding infectious complications in this patient population, more research is needed to evaluate infection complications in a subgroup of leukemic patients with prolonged neutropenia who often require repeat induction chemotherapy.

Methods: This was a retrospective analysis of 61 patients ages 18–85, between January 1, 2010 and March 14, 2018 who were diagnosed and being treated for acute leukemia. All selected patients experienced severe neutropenia (defined as absolute neutrophil count <500/μL) for ≥7 days. 33 patients underwent their first induction chemotherapy while 28 patients underwent repeat induction chemotherapy. Patient characteristics and infectious complications were examined. Analysis was performed to further study blood stream infections in this patient population.

Results: Sixty-one patients, mean age of …

Comparing Trends And Outcomes Among Hiv-Infected Vs. Hiv Uninfected Patients With Tuberculosis: A 5-Year Experience Within The Florida Department Of Health In Hillsborough County, Shylah M. Moore-Pardo, Anteneh Addisu, Tea Reljic, Sadaf Aslam, Beata Casanas

Internal Medicine Faculty Publications

Background: Although the rate of tuberculosis (TB) has significantly declined in the United States, elimination has plateaued. Florida is one of the states with the greatest number of cases. The majority of cases occur in foreign-born individuals. Human immunodeficiency virus (HIV) is also a major contributor. HIV-TB coinfection leads to reciprocal interactions with significant clinical impact. We aim to compare the risk factors, clinical findings, and outcomes among HIV-infected vs. HIV uninfected patients.

Methods: A retrospective cohort study of TB cases over a 5 year period (2012–2017) was conducted. All patients with HIV co-infection with age- and gender-matched HIV negative …

Predictors Of Recurrent Infective Endocarditis In Intravenous Drug Users, Kullatham Kongpakpaisarn, Sadaf Aslam, Musaab Tariq, Rahul Mhaskar, Doosup Shin, Chandrashekar Bohra, Charurut Somboonwit

Internal Medicine Faculty Publications

Background: Caring for hospitalized patients with infective endocarditis (IE) can be challenging due to the nature of the disease and its complications, underlying medical and psychiatric problems, socioeconomic status and environmental factors.Some of these patients develop recurrent IE after the first episode treated. On-going intravenous (IV) drug use after hospital discharge is the highest predictive factor for recurrent IE. Besides IV drug use, there are limited data of other contributing factors to recurrent IE. Those factors may be modifiable during the first hospitalization to reduce the incidence of recurrent IE.

Methods: A retrospective cohort study was conducted at a large …

Are Microclimate Conditions In El Malpais National Monument Caves In New Mexico, Usa Suitable For Pseudogymnoascus Growth?, Terry J. Torres-Cruz, Porras-Alfaro, Nicole A. Caimi

KIP Articles

White-nose syndrome (WNS) is a bat disease caused by the fungal pathogen Pseudogymnoascus destructans, which thrives in cold and very humid environments where bats frequently hibernate. Conidia of Pseudogymnoascus species are often documented on bats prior to the onset of WNS, but characterization of high-risk areas defined by microclimate cave conditions have been lacking. Investigating the occurrence of this fungal genus and appropriate environmental conditions to support P. destructans in southwestern U.S. caves is key to understanding the sites most likely to be impacted by WNS. Microclimate conditions in ten caves at El Malpais (ELMA) National Monument in New Mexico, …

2019 Children's Legislative Report Card, Children's Advocacy Institute, University Of San Diego School Of Law

Children's Legislative Report Card

No abstract provided.

Acceptability And Adequacy Of Vaginal Self Sampling For Hpv Dna Testing In Cervical Cancer Screening Among Women Attending A Tertiary Hospital Clinics In Nairobi Kenya, Sagal Omar Salad

Master of Medicine in Anatomic Pathology

Background: Cervical cancer is a main concern of women’s health globally. In Kenya, Cervical cancer is the second most common cancer in women and the leading causes of cancer related deaths. Several screening methods exist including cytology, human papilloma virus DNA test and visual inspection with Acetic Acid or Lugol’s Iodine (VIA/VILI). The current screening rate uptake in Kenya is poor, HPV DNA self-sampling may have a role in increasing the screening uptake as many studies have shown that self-sampling for HPV DNA testing is acceptable, though some others favoured over self-sampling. This study aims to assess whether vaginal HPV …

The Nursing History Of Ngala Since 1890: An Early Parenting Organisation In Western Australia, Elaine Bennett, Selma Alliex, Caroline Bulsara

Nursing Papers and Journal Articles

Background: This study was the first phase of a larger study which explored the past, present and future of nursing in early parenting services in Australia.

Aim: The aim of this paper is to describe the history of nursing within an early parenting service in Western Australia (WA).

Methods: Triangulation of multiple data sources was used to summarise the nursing role over 120 years. The history was discovered through a document analysis of archives, including oral histories, organisational documents, focus groups, nurses’ diaries and interviews with nurses.

Findings: The nursing role and context is described over three time periods: 1890–1960; …

The Lew.1wr1 Rat Model Is Glucose Intolerant, Genoah Collins

Theses

The susceptibility to the development of glucose intolerance in the young adult type 1 diabetes model LEW.1 WRl was investigated. The animals were given either a 10% fat 7% sucrose diet or a 45% high fat 21% sucrose diet for 10 weeks. Two glucose tolerance tests (GTT) and an insulin tolerance test (ITT) were performed at 0, 8, and 4 weeks, respectively. Terminal anesthetized blood glucose was measured before sacrifice . Serum obtained at harvest was used for a metabolic hormone and cytokine/chemokine multiplex array and ongoing metabolomics studies. Pancreata collected at harvest were used for histological examination of islets. …

Impacts Of Trogocytosis-Mediated Intracellular Signaling On Cd4+ T Cell Effector Cytokine Production And Differentiation, Steven James Reed

Graduate Student Theses, Dissertations, & Professional Papers

Trogocytosis is the direct intercellular transfer of membrane and membrane associated molecules. Unlike other passive-membrane transfer events, trogocytosed molecules may remain fully functional and become re-expressed on the surface of the trogocytosis-positive (trog+) recipient. This phenomenon commonly occurs between various cell types, including those of the immune system. CD4+ T cell trogocytosis occurs during their activation by antigen presenting cells (APC). Consequently, the acquired molecules include ligands for signaling receptors on the T cell.

The impacts of CD4+ trogocytosis on the immune response are largely unknown. While it has been demonstrated that trog+ cells can present trogocytosed peptide:MHC (pMHC), and …

Recovering Our Roots: The Importance Of Salish Ethnobotanical Knowledge And Traditional Food Systems To Community Wellbeing On The Flathead Indian Reservation In Montana., Mitchell Rose Bear Don't Walk

Graduate Student Theses, Dissertations, & Professional Papers

This thesis provides a culturally-comprehensive review of the plants utilized for food in the Bitterroot Salish tribe of northwestern Montana. As part of the larger Confederated Salish and Kootenai Tribes (CS&KT) of the Flathead Indian Reservation, the Bitterroot Salish historically utilized hundreds of plants for food, medicine and hygiene. This thesis aims to highlight food plants and their important cultural components. The information herein is a combination of history, ethnography, linguistics, ethnobotany, and first-hand experience with the current Salish community to provide a holistic framework of understanding traditional food plants today. A comprehensive plant list is provided with Latin, Salish …

Prevention Of Crystalline Silica-Induced Inflammation By The Anti-Malarial Hydroxychloroquine, Rachel Dawn Burmeister

Graduate Student Theses, Dissertations, & Professional Papers

Exposure to inhaled crystalline silica (cSiO2) is common in occupations where there is cutting, milling, or grinding of cSiO2 containing material. The Occupational Safety and Health Administration estimates that over 2 million workers may be exposed to inhaled cSiO2 in the United States. Inhalation of cSiO2 causes acute and chronic inflammation and may lead pulmonary diseases such as silicosis, as well as an increased risk of developing autoimmune diseases.Unfortunately, treatment of cSiO2-induced lung diseases is limited and primarily focused on supportive care.

Inflammation caused by cSiO2 begins when, cSiO2 particles are phagocytized by alveolar macrophages. Interaction between the particle and …

Performance Profile For Tabata Intermittent Training On Treadmill, Nathan Tamayo

Cal Poly Humboldt theses and projects

This study investigated the effect of supramaximal intensities on number of Tabata bouts performed by recreationally trained males. Eleven relatively trained men (mean age = 22.27 ± 1.74 years, height = 1.72 ± 0.07m, mass = 78.74 ± 15.77kg) were assigned the four intensities in a counterbalanced order. Subjects completed a warm up which was then followed by consecutive 20-second bouts of treadmill running with 10 second bouts of rest until fatigue given each intensity (110%,130%,150%,170% of VO_2max). Given that ideal number of Tabata bouts is ~8 with an intensity of 170% VO_2max, results suggested that …

Effectiveness Of Digital Media Technologybased Interventions On Hiv & Sti Risk Reduction Among Young People: A Metaanalysis, Paul Sherwin O. Tarnate, Keith Roger R. Serrano, Vanessa-Maria F. Torres-Ticzon, Kathlynne Anne A. Senen

Ateneo School of Medicine and Public Health Publications

Background: Prevention strategies delivered through digital media technology (DMT) have been developed to reduce HIV incidence among young people. However, no best-practice DMT intervention strategies exist in handling HIV prevention programs among young people. Objectives: To determine the effectiveness of DMT-based interventions in reducing risk-taking behaviours among young people that may predispose them to acquiring HIV and other sexually transmitted infections. Subjects and Selection Criteria: Randomized controlled trials and quasi-experimental studies with rigorous controls comparing DMT-based interventions and controls on reducing risk-taking behaviors among young people aged 10-24 years were included. Data Collection: Search methods were done on the following: …

Exploring Legal And Policy Responses To Opioids: America's Worst Public Health Emergency, James G. Hodge Jr., Chelsea Gulinson, Leila F. S. Barraza, Walter G. Johnson, Drew Hensley, Haley R. Augur

South Carolina Law Review

No abstract provided.