Digital Commons Network^™

Axon Initial Segment Integrity In Aging And Traumatic Brain Injury, Mazen M. Gouda

Theses and Dissertations

According to the Center for Disease Control’s (CDC) report to the Congress, there are 2.2 million emergency department visits; 80,000 hospitalizations; and 50,000 deaths each year due to traumatic brain injury. Adults 65 years and older account substantially for the majority of the hospitalization and deaths. Over 70% of the traumatic brain injuries of the older adults are classified as mild to moderate; however, even with these milder injuries, older adults present with a significantly higher morbidity and mortality compared to all other age groups (LeBlanc et al., 2006). With that in mind, it seems essential to develop a deeper …

Etiological Contributions Of Genetic And Environmental Factors To Nonmedical Use Of Prescription Drugs Among Young Adults, Cassie Overstreet

Theses and Dissertations

Key variables such as trauma exposure (TE) and internalizing symptoms (e.g., posttraumatic stress disorder [PTSD]) have been shown to correlate with non-medical use of prescription drugs (NMUPD); however, the temporal associations between these phenotypes remain poorly understood. Moreover, there is a paucity of research that incorporates the influence of genetic factors in the etiology of NMUPD. Although it has been demonstrated that drug use disorders are moderately heritable, research aimed at identifying the specific genes conferring risk is virtually non-existent for NMUPD. Therefore, determining the contribution of genetic and environmental factors associated with risk is critical to understanding NMUPD. To …

Factors That Influence Mental Health Services Utilization By Children Who Have Experienced Adversity, Mary B. Stebbins

Theses and Dissertations

Adverse childhood experiences (ACEs) are linked to increased mental health problems in children, but their association with mental health services utilization is not well known. This secondary analysis used 2016 National Survey of Children’s Health data from two samples: children aged 6-17-years-old with a mental or behavioral condition in need of treatment or counseling (N = 5,723); and a subsample of children who experienced at least one ACE (n = 3,812). Multiple logistic regression and latent class analysis (LCA) were performed to examine the association between ACEs and mental health services utilization. Multiple logistic regressions also examined the …

Latent Growth Model Approach To Characterize Maternal Prenatal Dna Methylation Trajectories, Dana Lapato

Theses and Dissertations

Background. DNA methylation (DNAm) is a removable chemical modification to the DNA sequence intimately associated with genomic stability, cellular identity, and gene expression. DNAm patterning reflects joint contributions from genetic, environmental, and behavioral factors. As such, differences in DNAm patterns may explain interindividual variability in risk liability for complex traits like major depression (MD). Hundreds of significant DNAm loci have been identified using cross-sectional association studies. This dissertation builds on that foundational work to explore novel statistical approaches for longitudinal DNAm analyses. Methods. Repeated measures of genome-wide DNAm and social and environmental determinants of health were collected up to six …

The Effects Of Notch Signaling On Functional Recovery Following Traumatic Brain Injury, Jyoti Lodha

Theses and Dissertations

2.5 million people sustain a traumatic brain injury (TBI) annually in the United States. Although there is potential for functional recovery following TBI, there is no definitive treatment to improve recovery after TBI. Our lab has shown that TBI enhances an endogenous neurogenic response in the subventricular zone and hippocampus. TBI-induced neural stem cells (NSCs) can integrate into regions such as the hippocampus and olfactory bulb. Although the mechanism behind TBI-enhanced neurogenesis remains unknown, the Notch signaling pathway has been implicated as a regulator in the maintenance and survival of NSCs.

This thesis explores the effects of Notch pathway manipulation …

Exploring The Standard: A Look Into Modern Voice And Speech Curriculum, Taylor L. Bosta

Theses and Dissertations

This thesis explores the current state of voice and speech education to discover if there is a standard of learning from university to university. By examining the history of voice and speech, discovering basic skills, breaking down teaching methodologies, and taking a look at current university and conservatory acting programs, the thesis discovers there is not a standard way of teaching, but there is a standard set of skills that should be upheld when planning voice and speech curriculum.

Illumination Of The Golgi Apparatus Of Pathogenic And Nonpathogenic Naegleria Species, Tyler M. Poe

Theses and Dissertations

In this study, Naegleria fowleri, a pathogenic amoeba and the causative agent of Primary Amebic Meningoencephalitis (PAM), was utilized to determine the presence or absence of classically conserved Golgi molecules featured in the expression of a Golgi apparatus. Previous studies concluded no Golgi expression via light microscopy and transmission electron microscopy, but a recent report on Naegleria gruberi indicated the presence of dispersed Golgi tubules. Non-pathogenic species of the Naegleria genus such as Naegleria gruberi 30540 and Naegleria lovaniensis 30569 were utilized in Western immunoblot analysis compared to reduced whole-cell lysate proteins of two strains of N. fowleri and …

When Empathy Only Goes So Far: Development Of A Trait Parochial Empathy Scale, Anna Maria C. Behler

Theses and Dissertations

Empathy, the ability to feel and/or understand another’s emotional state, plays a significant role in interpersonal interactions, mitigating hostility and enhancing affiliation and helping. However, empathy also biases interpersonal reactions. For example, at the group level empathy can become amplified towards members of their ingroup and blunted towards individuals in outgroups, a term called parochial empathy. Currently, no validated measures of parochial empathy at the dispositional level exist, and development of such a scale would be important to understanding the role of group-based emotions in prejudice and discrimination against outgroups. I conducted five studies to develop and validate a …

Analyzing Interactions Between Methcathinone Analogs And The Human Monoamine Transporters, Vy Nguyen

Theses and Dissertations

Psychostimulants elicit their psychological and behavioral effects through interactions with the monoamine transporters. These compounds are classified as either substrates or reuptake inhibitors, depending on their mechanism of action, and have varying potencies at the three human monoamine transporters, hDAT, hSERT, and hNET. Substrates and reuptake inhibitors also have distinct electrophysiological signatures: reuptake inhibitors cause hyperpolarization in HEK293 cells stably expressing the monoamine transporters, whereas substrate transport mediates a depolarizing current. Recent studies have shown that substrate-mediated depolarization is sufficient to activate L-type calcium channels, a characteristic which has been used to develop a novel calcium assay which uses calcium …

Assessment Of Microbial Carbon Processing And Its Implications To The Carbon Budget Of Lake Superior, Lindsey Michelle Koren

Theses and Dissertations

Over the past few decades, there has been increased research focus on carbon cycling within aquatic systems, especially with the changing global climate. Inland waters play a major role in the global carbon cycle, but the fundamental features remain poorly understood, particularly the large lakes of the world. Our experimental approach assessing the carbon budget of Lake Superior, the largest freshwater lake by area, provides spatial and temporal variability that has been previously overlooked but may be critical to our understanding on the biogeochemical processes controlling the lake. Multiple stations were chosen across the lake, both nearshore and offshore, to …

Construction And Validation Of A Non-Medical Use Of Prescription Opioids Outcome Expectancies Scale Among College Students In China, Cheuk Chi Tam

Theses and Dissertations

Background:Non-medical use of prescription opioids (NMUPO) has become a clear threat to public health. Young adults (aged 18 to 25) have a high risk of NMUPO. My prior work on Chinese undergraduates indicates a high prevalence of lifetime NMUPO (49.2%). Health behavior theories propose that outcome expectancies are robust psychosocial determinants of substance use. Literature has identified the influence of outcome expectancies on alcohol and drug use. However, the role of outcome expectancies in NMUPO in China is unknown, and a scarcity of a valid measures for NMUPO outcome expectancies may be a barrier. Our previous research also found …

Determining Success Factors In Interprofessional Practice With Dynamic Teams, Danah Alsane

Theses and Dissertations

Background: The interprofessional healthcare team is dynamic to cope with complex issues and needs existing in the healthcare environment. Understanding the important factors that predict dynamic team effectiveness helps health educators to establish team training models that focus on improving collaborative student performance that facilitates their transition to practice and helps to improve the health and system outcomes.

Objectives:1) Assess the impact of a practice-based interprofessional education (IPE) model on student attitudes and knowledge toward interprofessional teamwork.2) Evaluate predictors associated with team effectiveness among dynamic student teams participating in a practice-based IPE setting.

Methods: This was an exploratory cross-sectional …

Molecular Targets Of Psychedelics And Their Role In Behavioral Models Of Hallucinogenic Action, Hiba Z. Vohra

Theses and Dissertations

Psychedelics are a subset of hallucinogenic drugs that exert their characteristic effects through agonist activity at the serotonin receptor 2A (5-HT_2A). In this study, I aimed to characterize the modulatory role of the metabotropic glutamate subtype 2 receptor (mGluR2) in the 5-HT_2A-specific rodent model of hallucinogenic action, head-twitch response (HTR). Secondly, I aimed to explore if 5-HT_2A agonist-induced deficits in prepulse inhibition (PPI) of the startle response, an additional model of hallucinogenic action, could be produced in mice. Though 5-HT_2A agonist-induced PPI deficits, which represent interruptions in normal sensorimotor gating, have been described in …

Eralpha Isoforms Modulate The Tumorigenicity Of 24r,25(Oh)2d3 In Estrogen-Responsive Cancer, Anjali Verma

Theses and Dissertations

Over 200,000 cases of breast cancer are diagnosed every year. Nearly 20% of these patients supplement their diets with some form of vitamin D. This high frequency of vitamin D supplement use may be due in part to research suggesting that cancer patients with higher serum vitamin D₃ levels have better prognoses than patients with low serum vitamin D_3. However, double-blind clinical trials on the efficacy of vitamin D₃ supplementation in breast cancer have been inconclusive. A recent meta-analysis showed evidence of reduced cancer recurrence in patients taking vitamin D₃ supplements who had ‘estrogen receptor positive’ …

Cyclophilin A As A Molecular Switch Regulating Prolactin Receptor Mediated Signaling, Mammary Tumorigenesis And Metastasis, Shawn Hakim

Theses and Dissertations

Prolactin (PRL) and its receptor (PRLr) have been implicated in the development and progression of human breast cancer. PRL activates its receptor and induces activation of proximal Janus kinase 2 (Jak2) for signal transduction. Here, we sought to determine the role of PRLr-associated peptidyl-prolyl isomerase, cyclophilin A (CypA), in modulating structure/function relationships of the PRLr. It was demonstrated that CypA mediated PRL-induced conformational change of the CFP- and -YFP tagged forms of the PRLr cytoplasmic-tail, whereas CypA inhibition by NIM811 (N-methyl-4-isoleucine cyclosporin) or knockdown blocked the conformational change of the PRLr assessed by Fluorescence Resonance Energy Transfer (FRET) signal or …

Sensor Placement For Damage Localization In Sensor Networks, Fereshteh Firouzi

Theses and Dissertations

The objective of this thesis is to formulate and solve the sensor placement problem for damage localization in a sensor network. A Bayesian estimation problem is formulated with the time-of-flight (ToF) measurements. In this model, ToF of lamb waves, which are generated and received by piezoelectric sensors, is the total time for each wave to be transmitted, reflected by the target, and received by the sensor. The ToF of the scattered lamb wave has characteristic information about the target location. By using the measurement model and prior information, the target location is estimated in a centralized sensor network with a …

Down By Law: A Demographic And Geographic Analysis Of Those Killed By Police, Scott W. Murrah

Theses and Dissertations

After the rebellion over the killing of Michael Brown, the US Justice Department reported that over-policing for the sake of monetary extraction was taking place in Ferguson, MO, with non-White and people in poverty being disproportionately targeted at the hands of the police. And while it has been shown to be present within the Ferguson community, this extraction and targeting by police is not a geographically isolated occurrence. Based on previous research, a racialized, economic-based system of oppression goes hand-in-hand with policing. But how do the qualities of these geographies affect the prominence and location of police violence on a …

Preconditioning Of The Tumor Microenvironment By Means Of Low Dose Chemotherapies For An Effective Immunotherapy Of Breast Cancer, Hussein F. Aqbi

Theses and Dissertations

Breast cancer mortality is mainly due to distant recurrence of the disease arising from dormant tumor cells established by cancer therapies. Patients who initially respond to cancer therapies often succumb to distant recurrence of the disease. It is not clear why people with the same type of breast cancer respond to treatments differently; some escape from dormancy and relapse earlier than others. In addition, some tumor clones respond to immunotherapy while others do not. We investigated how autophagy plays a role in accelerating or delaying recurrence of neu overexpressing mouse mammary carcinoma (MMC) following adriamycin (ADR) treatment, and in affecting …

Dating Violence And Latina/O Emerging Adult’S Attitudes Towards Condoms And Condom Use Behaviors: Examining The Role Of Culture, Melissa Avila

Theses and Dissertations

Latina/o emerging adults living in southern states may be especially at risk of being disproportionately affected by HIV. In 2015, Latina/os aged 20 to 24 accounted for 14.7% of all 20-24-year old’s living with HIV in southern states, despite the fact that 20 to 24-year-olds make up 3.7% of the region’s overall population of individuals living with HIV (CDC, 2016; U.S. Census Bureau, 2017). Specifically, there continues to be groups of Latina/o emerging adults who do not use condoms when they are sexually active and who are not using condoms consistently. Condom use literature suggests that relationship factors, such as …

Understanding The Impact The Hospital Readmission Rate Program And Value Based Purchasing Has Had On The Financial Viability Of Academic Health Centers, 2011 To 2015., David Allen

Theses and Dissertations

Academic Health Centers (AHCs) hold a unique place in today’s health care environment. They service their communities through a tripartite mission of education, research, and provision of complex care to disadvantaged populations. To achieve this mission, AHCs face challenges in funding and cost containment compared to non-AHCs. Additionally, the implementation of government programs like the Hospital Readmission Rate Program (HRRP) and Value Based Purchasing (VBP) have the potential to affect AHCs differently from non-AHCs. While AHC’s unique features are known and there has been research to date on HRRP and VBP, literature has yet to statistically explore the financial differences …

The Role Of Cellular Aging In The Regulation Of Free Fatty Acid Induced Cellular Inflammation By Liver X Receptor Alpha, Charles S. Schwartz

Theses and Dissertations

Purpose: This study examined the impact of an acute bout of exercise on LXRα mRNA expression in isolated PBMCs from aerobically fit middle-aged males, and its associations with cellular inflammation. Furthermore, association between cellular age and LXRα were investigated. Methods: LXRα mRNA, plasma free fatty acids, IL-6, and MCP-1 responses were quantified following acute moderate intensity exercise and throughout recovery. To examine LXRα’s associations with cellular inflammation, PBMCs were stimulated with palmitate. Finally, telomere length was quantified as an indicator of cellular age. Results: LXRα mRNA expression was reduced at 90 minutes of recovery and did not influence systemic inflammation. …

Getting To Zero Preventable Falls: An Exploratory Study, Kate Lim

Theses and Dissertations

Objective: The objective of this study is to examine relations between patient safety culture and processes of care, specifically, how patient safety culture influences the prevention of patient falls. The purpose of this inquiry is to identify the barriers and facilitators that can advance an inpatient rehabilitation facility to become a high reliability organization and advance interdisciplinary teamwork.

Method: A qualitative phenomenological approach was conducted and an interpretive phenomenological analysis explored the experiences of frontline staff with regard to patient safety culture and fall prevention. The study utilized semi-structured interviews with 24 frontline staff from three inpatient rehabilitation hospitals. Participants …

The Effect Of Change In Medi-Cal Dental Coverage On Dental Care Utilization Among Medi-Cal Beneficiaries, Min H. Zhang

Theses and Dissertations

One of the most important factors in accessing dental care is having dental insurance. For people with low incomes, Medicaid is the main source of health insurance. Medi-Cal is California’s Medicaid program. Adult dental services were mostly eliminated in Medi-Cal in 2009 due to the economic downturn and partially restored in 2014. The objective of this study is to evaluate the effect of change in Medi-Cal dental coverage, specifically the partial restoration of adult dental coverage in 2014, on dental care utilization among Medi-Cal beneficiaries. The partial restoration significantly increased the utilization rates in dental clinics from 2014 to 2017 …

Genome-Wide Systems Genetics Of Alcohol Consumption And Dependence, Kristin Mignogna

Theses and Dissertations

Widely effective treatment for alcohol use disorder is not yet available, because the exact biological mechanisms that underlie this disorder are not completely understood. One way to gain a better understanding of these mechanisms is to examine the genetic frameworks that contribute to the risk for developing this disorder. This dissertation examines genetic association data in combination with gene expression networks in the brain to identify functional groups of genes associated with alcohol consumption and dependence.

The first study took advantage of the behavioral complexity of human samples, and experimental capabilities provided by mouse models, by co-analyzing gene expression networks …

A Longitudinal Study Of The Stress-Buffering Effects Of Romantic Relationships On Alcohol Outcomes In College Students Exposed To Trauma, Rebecca L. Smith

Theses and Dissertations

This study examined interplay between interpersonal trauma (IPT), relationship status, relationship satisfaction (SAT), and partner substance use (PSU), and whether these relationship dimensions moderated associations between IPT and alcohol outcomes. Data came from a longitudinal study of college students (N=9,911; 61%female; 49%White). Precollege IPT increased the likelihood of being in a relationship, while college-onset IPT decreased the likelihood. IPT predicted lower SAT and higher PSU. Individuals with precollege IPT consumed more alcohol than those without IPT, but this was mitigated for those in relationships. Individuals with college-onset IPT consumed more alcohol than those without IPT, and this was …

Differential Mobility Classifiers In The Non-Ideal Assembly, Thamir Alsharifi

Theses and Dissertations

The differential mobility classifier (DMC) is one of the core components in electrical mobility particle sizers for sizing sub-micrometer particles. Designing the DMC requires knowledge of the geometrical and constructional imperfection (or tolerance). Studying the effects of geometrical imperfection on the performance of the DMC is necessary to provide manufacturing tolerance and it helps to predict the performance of geometrically imperfect classifiers, as well as providing a calibration curve for the DMC. This thesis was accomplished via studying the cylindrical classifier and the parallel plate classifier. The numerical model was built using the most recent versions of COMSOL Multiphysics^{® …}

The Increased Frequency Of Micronuclei Seen In Women With A History Of Childhood Sexual Abuse Reflects More Numerical Than Structural Acquired Chromosomal Events: A Discordant Identical Co-Twin Study, Kaitlyn M. Dochelli

Theses and Dissertations

Childhood sexual abuse (CSA) is a stressful life experience with lasting/far-reaching health and psychopathological consequences. Our laboratory recently identified a significantly increased frequency of acquired chromosomal anomalies (assessed using the cytokinesis-blocked micronucleus assay) in adult female twins exposed to CSA when compared to their unexposed co-twin. The primary aim of this study was to evaluate potential mechanism(s) underlying the observed increases in levels of micronuclei in an expanded group of 90 female identical twins (61 CSA+ females and 29 CSA- females [including a total of 27 MZ co-twin pairs]) using fluorescence in situ hybridization (FISH) methodologies, with PNA probes specific …

Modulation Of Autophagy And Senescence To Enhance The Response To Therapy In Triple Negative Breast Cancer, Liliya Tyutyunyk-Massey

Theses and Dissertations

Abstract

Although great strides have been made over the decades in development and optimization of anti-cancer therapies, even highly effective drugs often fail to completely eliminate tumors. Residual tumor cells can enter into a state of dormancy for prolonged periods of time but eventually are able to regain proliferative capacity and reemerge as chemotherapy-resistant disease. Because recurrent disease is a leading contributor to patient’s mortality, it is paramount to identify strategies for effectively destroying residual tumor cells.

Cytotoxic drugs and ionizing radiation are used as standard therapies in a variety of cancers. These modalities induce apoptosis, autophagy and senescence. Senescence …

Cold X-Ray Effects On Satellite Solar Panels In Orbit, Myles Fogleman

Theses and Dissertations

An exo-atmospheric nuclear detonation releases up to 80 percent of its’ energy as X-rays. Satellite’s solar cells and their protective coatings are vulnerable to low energy X-ray radiation. Cold X-rays (~1-1.5 keV) are absorbed close to the surface of materials causing the blow-off and rapid formation of Warm Dense Plasmas (WDPs), particularly in a gap between the unshielded active elements of solar cells. To understand how WDPs are created, it is necessary to investigate the power density distribution produced by cold X-rays for typical solar panel surface materials. The Monte Carlo stepping model implemented in the GEANT4 software toolkit is …

A Longitudinal Investigation Of Interpersonal Trauma Exposure, Posttraumatic Stress Disorder, And Cannabis Use Phenotypes Among College Students, Terrell A. Hicks

Theses and Dissertations

College students have an increased risk for cannabis use, trauma exposure, and posttraumatic stress disorder (PTSD). Cannabis use disorder (CUD) and PTSD comorbidity is high, and given the negative consequences of the comorbidity (e.g., poor academic outcomes), there is a need to understand comorbid CUD-PTSD etiology. Two primary etiologic models exist: self-medication (i.e., PTSD à CUD) and high-risk (i.e., CUD à PTSD) hypotheses. This study 1) examined the prevalence and predictors of cannabis use and interpersonal trauma (IPT) exposure; 2) investigated the relationship between cannabis use and IPT; and 3) examined cannabis use, IPT, and PTSD through mediational self-medication and …